CS238887B1 - A method for producing polypeptides from blood - Google Patents

Abstract

The invention relates to a method for obtaining polypeptides from blood. The essence of the invention is that the collected slaughter blood is precipitated with ethanol flowing in countercurrently and the precipitated xerogel is either immediately or after suitable storage treated with sodium hydroxide to form a peptide filtrate, which is reprecipitated in countercurrently with ethanol and the polypeptides obtained are separated and dried. The polypeptides obtained can be used as additives, carriers of proteins or amino acids in food.

Description

The invention relates to a method for obtaining polypeptides from blood.

The current methods of processing the blood of animals for slaughter have encountered a number of problems associated with hygiene collection, storage and aseptic processing. Abroad, this problem is solved by severe hypothermia of blood while producing ice, but this requires a great deal of energy and equipment costs. In addition, there is always a risk that the blood will be infected with pathogenic microorganisms during processing. Although blood coagulation by ethanol is known on a laboratory scale, a similar method of processing has not been used on an industrial scale.

The object of the present invention is that the collected slaughter blood flows into a countercurrent column against incoming ethanol, where at a temperature of up to 40 ° C and at the isoelectric point, the colloid-bound water is removed and the xylogel is formed from the lyophil of the protein. The blood sediments at the bottom of the column from where it is continuously collected. The blood, after filtration, the residual množstvem ethanol, may be stored in closed packages for a long time or immediately s'a spracútá ^l ná'peptidy.

It is first treated with a 10% sodium hydroxide solution while boiling until all the hydrolysable parts of the blood have dissolved. The residue is filtered off, neutralized with hydrochloric acid and used as feed. The filtrate is pumped back to a countercurrent desolvation column where polypeptides are precipitated with ethanol. The sedimented polypeptides are filtered off, freed of ethanol in a vacuum oven and packaged hygienically.

Advantages of the method according to the invention are that the blood collected does not have to be processed aseptically or not aseptically. strong cooling, thus saving energy and eliminating the risk of subsequent infections with pathogenic but also other microorganisms.

The method of preparation is illustrated in the following examples:

Example 1

100 kg of slaughter blood are successively injected into the countercurrent column at the top and 200 kg of 96% ethanol are fed countercurrently. The sedimented desolvated blood (xerogel) is collected continuously, free of ethanol on a filter. 60 kg of precipitated protein are obtained with a water and ethanol content of 44 kg, the ethanol content of which is about 45%, i.e. about 50%. j. 19.8 kg of ethanol. The desolvated protein obtained, which can be recovered due to the high ethanol content, can be stored in sealed containers.

Example 2

The desolvated blood obtained according to Example 1 can be processed either directly or after removal from storage containers as follows: 10 kg of desolvated blood containing 30 kg of protein in the dry state are treated with 100 kg of 10% sodium hydroxide solution. boiling until all the hydrolyzable portions of the proteins have dissolved. Filter and neutralize the precipitate by washing with hydrochloric acid. The filtrate is again injected successively into a countercurrent column as in Example 1, and 20 kg polypeptides, calculated on a dry weight basis, are precipitated in countercurrent with ethanol. The polypeptides are filtered off and freed from ethanol and water in a vacuum oven and packaged.

The product obtained is an off-white powder, a pleasant taste, swells in water and dissolves in excess water.

Claims (1)

A method for producing polypeptides from blood, comprising the action of ethanol on the blood of slaughter animals, characterized in that the slaughtered blood is precipitated by counter-flowing ethanol at a temperature of 28 to 40 ° C, the precipitated xerogel is separated and stored either in sealed containers. After processing, or after storage, 10% sodium hydroxide at boiling point of the mixture to dissolve the hydrolyzable protein portions and, after filtration with ethanol, precipitates polypeptides in the filtrate, which are separated and dried.