CS245985B1 - Coupling capillaries in an isotachophoresis apparatus with connected columns - Google Patents

Abstract

The solution concerns the connection of capillaries of different diameters in an isotachophoresis apparatus with connected columns. The first capillary /7/ is arranged in the shell of a container /1/ with electrolyte /2/, which is provided with a venting and filling opening /8/ and a supply electrode /3/. The second capillary /6/ is placed in a pressure member /5/. A semipermeable membrane /4/ is placed in the seat /9/ of the container /1/, on which the pressure member /5/ is tightly placed. The opening in the semipermeable membrane /4/ has the same internal diameter as the second capillary /6/. The solution is usable in devices for capillary isotachophoresis with a larger number of capillaries and a flexible combination of electrolyte systems.

Description

(54)

Connection of capillaries in the isotachophoresis apparatus with coupled columns

The first capillary (7) is arranged in the housing of the container (1) with the electrolyte (2), which is provided with a venting and filling opening (8) and an inlet electrode (3). . The second capillary (6) is embedded in the presser (5). In the receptacle (9) of the container (1) a semi-permeable membrane (4) is placed, on which the press-fit member (5) is tightly mounted. The orifice in the semipermeable membrane (4) has the same internal diameter as the second capillary (6).

The solution is applicable to capillary isotachophoresis devices with multiple capillaries and flexible combination of electrolyte systems.

245 985

245 985

The invention relates to the coupling of capillaries of an isotachophoresis apparatus to coupled columns.

The connection of capillaries of different diameters in isotachophoresis devices with coupled columns was previously solved by a system of channels and gaps, which was very demanding in design and manufacture. Another problem was to vent this complex system.

These drawbacks are eliminated by the capillary connection of the isotachophoresis apparatus to the coupled columns, wherein the first capillary is arranged in the housing of the electrolyte container provided with the venting and filling opening according to the invention. It is based on the fact that the press-fit element in which the second capillary is housed fits tightly into the seat of the container in which the semi-permeable membrane is provided with an opening having a diameter equal to the inner diameter of the second capillary.

The invention substantially simplifies the construction of the apparatus, creating conditions for direct connection of functional assemblies with little possibility of creating and trapping air bubbles. The solution according to the invention also greatly simplifies the preparatory handling, since it shortens the flushing of the capillary system, the flushing is now carried out for the entire system together up to the end of the last capillary. The solution is particularly suitable for the all-PTFE version, ie for work in organic solvents.

The invention and its effects are explained in more detail in the description of an exemplary embodiment thereof according to the accompanying drawing, which shows in cross-section the connection of the capillaries of the isotachophoresis apparatus with the coupled columns according to the invention.

2 245 985 a first capillary 7 of smaller internal diameter than the second capillary 6 is arranged in the housing of the electrolyte container 2, which is provided with a venting and filling opening 8 and a lead electrode χ. The second capillary 6 is housed in the press plate 5. A semi-permeable membrane 4 is mounted in the seat of the container 1, on which the press plate χ is tightly mounted. The opening of the semipermeable membrane 4 has the same inner diameter as the second capillary 6.

The invention is applicable to capillary isotachophoresis devices with a plurality of capillaries and a flexible combination of electrolyte systems.

Claims (1)

SUMMARY OF THE INVENTION Capillary connection in an isotachophoresis apparatus with coupled columns, wherein the first capillary is arranged in the housing of an electrolyte container having a venting and filling opening and a lead electrode, characterized in that the second capillary (6) is housed in a pressurizing member (5) which is tightly seated in the seat (9) of the container (1), in which a semipermeable membrane (4) is disposed with an opening equal to the inner diameter of the second capillary (6).