CS249900B1 - Method of l-lysine preparation - Google Patents

Abstract

Method of preparing L-lysine by drying lysine fermented soil in a spray dryer, in which waste penicillin mycelium is used as a filler for good drying and increasing the quality of the resulting product. The method is carried out in such a way that an aqueous suspension of penicillin mycelium with a dry matter of 5 to 15% by weight is added to the thickened lysine fermented soil, optionally unthickened, before drying, the degradation of the residual penicillin was carried out by adding alkali to a pH value of 8 to 13, with stirring, in an amount of 5 to 50% by volume, calculated on the volume of the lysine fermented soil. The L-lysine produced in this way is used for the preparation of feed concentrates intended for animal nutrition.

Description

249900

The present invention relates to a process for the preparation of L-lysine by the fermentation broth for sprayers. The importance of essential amino acids, mainly lysine, is currently increasing in the fattening of farm animals. There are several methods of preparing lysine, both in pure form, and in admixture with other amino acids. These include, in particular, the isolation of natural substances, the chemical synthesis, the combination of chemical synthesis from caprolactam and the subsequent microbiological transfer of racemic lysine to the active 1-form and the bio-synthesis of L-lysine on liquid nutrient media by submerged cultivation with suitable production The most common method is the production of L-lysine by submerged culture, i.e.. by the fermentation route.

Lysine from the fermentation broth is usually recovered into the final product in the form of L-lysine. (HCl, 2 H2O by a multi-stage, relatively expensive isolation which ends with the crystallization of the concentrated eluate obtained from ion exchange columns).

The use of ions in the isolation of L-lysine is described in many patents, e.g. -austr. Patent No. 404,209, belg. patentč. 683 933, franc. U.S. Patent No. 2,041,658 and No. 033,845, MS. In other patents, detailed conditions for the preparation of eluates, thickening, crystallization and processes for further processing into crystalline formulations, e.g. No. 1,186,988, owls. pat. No. 174,940 and 279,621.

Principle of MS. AO No. 175 650 and MS. No. 217 280 is a method for producing L-lysine by drying the concentrated eluates onto a spray dryer, the advantage of which is the isolation process due to its shortening to crystallization and subsequent processing of the liquors. Further enhancement of the production efficiency of L-lysine is brought about by processes whose principle is the drying of non-concentrated or thickened fermentation soil on spray dryers, e.g. pat. No. 2 244 817, austr. pat. No. 314 959, jap. pat. No. 31 794, pol.pat. No. 98 183, MS. AO No. 162 214.

The procedures of the individual patents address the conditioning and thickening of the fermentation broth prior to drying, the use of different types of spray dryer, the drying process, the addition method and the type of use of the auxiliaries (drying agent).

The effectiveness of L-lysine production processes by drying on spray driers depends on the use of suitable auxiliaries (fillers whose availability and cost can be decisively influenced by the economic assessment of the process. Another disadvantage of the use of inorganic fillers is their negative impact on the drying apparatus due to the mechanical opening of the machine, and the known drying processes of the fermented lysine soil are known to have a drying process. it is dependent on the dry matter content of the soil, which is predominantly anesthetized by the biomass-producing microorganism, which is directly proportional to the dry matter content of the fermented soil.

Also known from the spray drying theory is the fact that, when using the filler, in which case the biomass is dead, the evaporation of water from the whole volume of the droplets dropped substantially, without the filler, resulting in beneficial quality effects. the dried product, ie the product is free-flowing, non-sticky.

Since the biomass content of the fermentation broth is determined by the conductivity of the fermentation process in which the lysine content is decisive, the maximum biomass content is limited and ranges from 4 to 6% by weight. Said biomass content is not sufficient for optimum drying, therefore it is necessary to add an additional filler to the fermented soil before drying, for example micronised limestone.

It has been found that L-lysine can be prepared by drying the fermented lysine, in the presence of a filler, on a spray dryer according to the invention, characterized in that the fermented lysine base, optionally concentrated to 35 to 50 wt. , dry matter content and 15 to 25 wt. L-lysine, prior to drying, add an aqueous suspension of 5% to 15% by weight of penicillin mycelium in which the degradation of residual penicillin was added by addition of alkali, e.g., hydroxamide ammonium or sodium to a pH of 8-13. with stirring, in an amount of 5 to 50% by volume, calculated on the volume of lysine soil, after which the fermented lyophilized soil is dried. An advantage of the process of the present invention is that an aqueous suspension of 10% by weight of penicillin mycelium is added to the unfermented lysed lysate to convert the residual degradation of the phenyleniline to pH 9.5 by addition of ammonium hydroxide or sodium hydroxide. , using a volume of 1 h, in an amount of 10% by volume, the volume of the uncoated lysine soil used. A further advantage of the process of the invention is that the content of lysine broth concentrated to 35 to 50% by weight is a dry content and 15 to 25% by weight. L-lysine, an aqueous suspension of penicillin mycelia of 10% by weight is added, whereby the residual penicillin addition of ammonium hydroxide or sodium is added to pH 9.5, with stirring for 1 h, at 249900 25% volume, calculated on the volume of thickened lysine soil. The penicillin mycelium added to the lysine prior to drying has a beneficial effect on the spray drying process and the quality of the resulting product. Correction of pH with alkali, e.g., ammonium hydroxide, and stirring of a penicillin mycelium suspension is sufficient to cause penicillin degradation.

In contrast to the possible methods of utilizing penicillin mycelia, e.g., for composting, drying the mycelium itself for feed purposes in the field or as a source of nutrients after prior hydrolysis of the fermentation technology, the invention also has the advantage that more effective evaluation of waste secondary raw material, as opposed to other requirements for the demands on technology and technology and technology. The advantage of this process is also the increased value of the final product due to the content of 15 to 20% by weight. of digestible proteins in a separate dry-penicillin mycelium.

Composition of penicillin mycelium in dry matter: total nitrogen 59,38 g / kg NH4 + 5,69 g / kg protein 231,0 g / kg protein digestible 180,0 g / kg ash 13,49% traces sulfate chlorides 1,2 g / kg

According to the process of the invention, a lysine can be produced, for example, by adding 10% by volume of a 10% w / w aqueous suspension of penicillin mycelium to the fermented lysine base, which is adjusted to a pH value of a concentrated ammonium hydroxide / sodium hydroxide solution. 9.5 and stirred for 1 h. The resulting suspension was concentrated to a dry weight of 40% by weight. and drying the drying oven. The resulting product is loose, non-sticky, non-hygroscopic, with a 30% wt.

In order to produce the present invention, a lysine product of 30-45% by weight can be produced from the fermented lysine soil using the waste penicillin mycelium in a simple manner, which meets all the requirements and has an increased nutritional value. compared to products using inorganic fillers. The produced L-lysine is used in the preparation of lysine concentrates for animal nutrition. The following examples of the invention do not limit, but merely supplement, the present invention. concentrated ammonium hydroxide was added to pH 9.5 with stirring, and the suspension was stirred for 1 h; A 50 ml sample of the suspension was neutralized with 20% by volume sulfuric acid at pH 7.0, the mycelium filtered out of the penicillin content by the biological method. No trace of penicillin has been determined in mycelia. The residue of the suspension is added to a 20 L fermented lysine base with an L-lysine content of 65 g / L. This mixture was concentrated to a circulating evaporator to give a dry matter content of 40% by weight. and drying the quench with a spray dryer, at an inlet air temperature of 180 ° C and an outlet temperature of 95 ° C. 2920 g of product are obtained, with a content of 37.7% by weight. L-lysine. The resulting product was free-flowing, non-sticky, non-hygroscopic, with a water content of 5.0% by weight, an ash of 10% by weight. Example 2

The procedure was the same as in Example 1, except that 40% by weight sodium hydroxide was added to a pH of 9.5 to a penicillin water suspension in water. 2 950 g of product are obtained with a content of 36.5% by weight. L-lysine. The resulting product was free-flowing, non-sticky, non-hygroscopic, with a water content of 5.4% by weight, an ash of 12.1% by weight. EXAMPLE 3 K 2 L of a Penicillin Mycelium Suspension in a Duct of 10% by weight. concentrated ammonium hydroxide was added with stirring to a pH of 9.5 and the suspension was stirred for 1 hour. No trace of penicillin was determined in the 50 ml sample taken. The residue of the suspension added to the 8 L thickened lysine solids of 38% by weight. and an L-lysine content of 158 g / l. The pH of the mixture was 6.9 after stirring. This blend was dried on a quarter-operating spray dryer at an inlet air temperature of 180 ° C and an outlet temperature of 95 ° C. 2890 g of product are obtained with a content of 36.7% by weight. L-lysine. The resulting product was free-flowing, non-sticky, non-hygroscopic with content. % of soil, 5.0% by weight, ash 10.4% by weight. Example 4

The same procedure as in Example 3, except that 40% by weight sodium hydroxide was added to a pH of 9.5 to a suspension of penicillin bicarbonate in water. 2930 g of product are obtained with a content of 35% by weight. L-lysine. The resulting product was Syrian, non-sticky, non-hygroscopic with a water content of 5.1% by weight. and ash 12.3 wt. K 2 1 penicillin mycelia suspension

Claims (3)

243900 PREDMET A process for the preparation of L-lysine by drying the fermented lysine soil, in the presence of a filler, characterized in that the lysine base, optionally concentrated to 35 to 50% by weight, is present. solids content of 15 to 25 wt. L-lysine, before drying, an aqueous suspension of 5% to 15% by weight of penicillin mycelium is added as a filler in which the degradation of residual penicillin by addition of alkali, ammonium or sodium hydroxide, pH 8 to 13, with stirring , in an amount of 5 to 50% by weight, calculated on a volume of lysine soil, whereupon the thus treated lysine soil is dried. 2. Sposoib as claimed in claim 1, characterized in that an aqueous suspension of the inventive ciline cellulose having a solids content of 10% by weight is added to the non-concentrated fermented lysine base and the degradation of the residual penicillin by addition of ammonium hydroxide or sodium hydroxide is added to pH 9.5, stirring for 1 h, at 10% by volume, calculated on the volume of the uncoated lysine soil. 3. A process according to claim 1, wherein the fermented lysine is concentrated to 35 to 50 [deg.]. % solids and 15 to 25% wt. L-lysine, a 10% w / w penicillin mycelium suispensia, in which the degradation of residual penicillin by the addition of ammonium hydroxide or sodium hydroxide to pH 9.5 was carried out, with stirring for 1 h, at 25% vol. thickened lysine soil. Severografla, n. P., Race 7; Most Price 2,40 Kčs