CS258420B1 - A chamber for circular chromatography - Google Patents

Abstract

The chamber for circular chromatography contains a cuvette, placed in an annular body with a cover, a chromatography plate and a device for supplying the mobile phase to the sorbent layer with a central and lateral capillary channel. The chamber is provided with a ring-shaped insert, which forms a cavity of capillary cross-section with the body, which is in contact with the sorbent layer of the chromatography plate on one side and is connected to the annular well on the other side by a central capillary channel.

Description

1 OIBÍCAHHE H30BPETEHHB TO ABTOPCKOMY CBHBETEJIbCTBY3aasne: 11.06.823aaBKa (ø 348 ^ 624 / 23-25MKH4 G 01 N 31/08

Abtopu: JI.A.EjiexepT, EnEpMaxoBa, WKKHceneB, Φ.Η.ΡΟΜβΗΟΒ 3βΗΒΗΤβηι »ί HayMHo-TexHunecKoe oCbeAHHeHHe AH CCCPHassaHHe Η9θ6ρβτβΗΗΛ: ΚΑΜΕΡΑ flJIH ΚΡΥΓΟΒΟΑ ΧΡΟΜΑΤΟΓΡΑΦΗΗ Η9θ6ρβΤβΗΗβ OTHOCHTCH Κ aHaJIHTHHeCKOň ΧΗΜΗΗ B HaCTHOCTH XpOMaTOrpaiJjH K-wecKOMy aHanusy h μοχθτ 6biTh HcnonbsoBaHo xpoMaTorpa <l> HpoBaHHH this-cnoňHwx nnacTHH MeTOflOM xpyroBoft χροΜβτοΓρβφΗΗ.

Hseecraa xaMepa ahh'προΒβΑβΗκη xpyrosott χροΜβτοΓρβφΗΗ, cocToaman hs V-oCpasHoft κηΒβτΜ, Ha xoTopyra ropKsoHTajibHo> cnoeM bhhs, ycTaHaanHBaeTCH xpo ·, MaTorpatoraecKax nnacTHHa. Ποαβηχηβη (Jjaaa nonaeTCH β ηεπτρ nnacTHHbí c πομο-ntbio HacocHoro ycTpoňcTsa nepes nnaTHHOHpHAHeBbiň xanHnnap (1). FlaHHaa xaMepa πο3βοηηθτ προβοαητβ χροΜβτοΓρβφΗροΒβΗΗβ αβυμη ΜβτοΑβΜκ: ΜβΤΟβΟΜ KOJIbUeBHX 3OH NPH HaHeceHHH npo6bl Β ΐχβΗΤρ nnacTHHbí H MeTOflOM paflH-i ajibHux CH3OH NPH HaHeceHHH npotiu b BHfle τοηθκ no pagnycy Ha acβκοτοροΜ paccOH-hhh ot AeHTpa. 3th flBa MeTofla nuipoxo HcnojibsytoTCH she axcnpecc-aHannsa pas * 4JiHHHhix cMeceň.

HegocTaTKOM mstoaob xonbueBbix h paAHanbHux god HBJíaeTCH το, ητο. b npo-yecce χροΜβτοΓρβφκροΒβΗΗΗ soHbí no Mepe npoflBHxeHHH no cnou nnacTHHbí pacmH-paeTca BcneACTBKe αηΦΦυβκη.

HsBec-r.eH μθτοα xpyroBoft χροΜβτοΓρβφπΗ - neHTpocTpeMHTejibHbiň, cornacHO. KOTOpoMy npoCa c noMonibw cneijHajibHoro ycT leastcTBa HaHOCHTca b βηαθ cnnoniHoňπηηη ηπη Towex Ha BHemHioio xpyroByu hhhhu cTapTa. Ποαβηχηηη 4> asa noflaeTCHot πβρΗφβρπΗ nnacTHHbí κ ueHTpy (2).

Πρκ 3TOM MeTOfle no Mepe προαβηχθηηη ποαβηχηοΑ φΒ3Μ x ueHTpy npoucxoAHT

BospacTaHHe cxopocth ποαβηχηοΑ φ33Μ h cyweHHe soh paaflenaeMwx κομπΟηθητοβ 258420 2 cMecM. nocjjeflHee oScToaTenbcTBO βμββαηο KOHijeHTpauneii aHanHSHpyeMbix BemecTBb MajiOM oGteMe pacTBopHTena.

HanOonee 6ηΗ3κοή κ H3o6peTeHwo HBnaeTca KaMepa, BKntonaiomaH κιοΒβτγ, pACN-b noxeHHyio KonbyeBOM Kopnyce c κρωιπκοή, xpoMaTorpa $ nuecKyra nnacTHHy h YCT-poňcTBO noflaw noflBHWHoň φβ3Μ κ cnoio copSeHTa ueHTpajitubíM c h Sokobhm KaiiHn-napHbíMH KanajiaMH (3).

HeflocTaTKaMH HSBecTHotó KaMepw hbhhiotch cjiowhoctb nonyaeHHa Kpyrnwx Tohko-cnoňHbix xpoMaTorpa $ HMecKHx nnacTHH and taxxi nnoxaa xHMHnecKaa ycToňnHBOCTbno ΟΤΜΟΠΙ6ΗΗΙΟ κ pacTBopaM noflBHXHOň φθ3Η (opraHHaecKHe h HeopranHnecKHe KHC-NotB) noflaB / iHiomero 6onbniHHcTBa MaTepnanoB H3 κοτορωχ HsroTOBnaioTca οβτκκ b peaynbTaTe aero προηοχοβητ sarpasnemie noflBHXHoň φβ3Η npoflyKTaMH peaKip-in, MTO npHBOflHT K HCKaJSeHHJO pe3yjJbTaTOB ΧρΟΜΗΤΟΓρΗφΗΗβϋΚΟΓΟ and Hajmaa. Iíenb Η3ο6ρβτβΗΗΗ - paciaHpeHHe oSnacTH πρΗΜβΗβΗΗΗ h ynyameifHe KaaecTBanpoBefleHHH χροΜβτοΓρβφηηβοκοΓΟ anajiH3a. YKa3ahHaH uenb uocTHraeTCH τβΜ, Uto KaMepa nna Kpyroeoft χροΜβτοΓρβφΗΗ, coflepxamaa KioBeTy, pacnonoxeHHyio b KÓnbueBOM Kopnyce c κρωωκοή, xpoMaTorpa-φΗΜβοκϊπο nnacTHHy h ycTpoftcTBO nonaan nouBHXHoft φ33Μ κ cnoio copSeHTa c ueHT-panbHblM H COKOBblM ΚβΠΗΠ, ΠΗρΗΜΜΗ KaHanaMH, CHaSweHa KOJlblXeBblM φΗ ^ ρΗΜΜ BKJiaflbl "MEM oSpaayioiUHM c KopnycoM nonocTb KanHnnapHoro cenenna, KOTopaa what ^ noňCTOpOHbt HaXORHTCH B ΚΟΚΤβΚΤβ CO CJlOeM COpSeHTa Χρ0ΜβΤ0Γρ3φΗηβ0Κ0ίί nnaCTHHbl ac flpyroň cropoHbí coeflHiiena c KonbueBbíM peeepByapoM ueHTpanbHWM KanHnnapHbíMKananoM, κοτορωϋ cbh33h c KonbueBoň nonocTbio nepes ycTpoftcTBO nouanH πο, πιβηχ-Hoft φβ3ω, NPN one hundred meters KanHnnapHbie KaHanbi konbuesoro pe3epsyapa o6pa3yioT pery-jihtop nepeMemeHHH snioeHTa.

Iloflaaa noflBKXHoň (Jiaahi nepe3 KanHnnapHbtň Kahan ποβΒΟππβτ ncnonb3OBaTbnnacTHHbí neTbipexyronbHoň φορΜΜ, H3roTOBneHHe κοτορωχ not npencTaBnaeT Tpyfl-HocTeft, NPH Hcnojib3OBaHHH cnennajibHbix aBTOMaTnnecKHX ycTpoftcTB.

KpoMe Toro, oTcyrcTBue TpaHcnopTHoro ycT leastcTBa rioflBnxHoň φβ3Μ κ cnoiocopCeHTa, BbinonHaeMoro b BHrte MeTannHnecKoft οτβΗΚΗ, noseonaeT H36exaTb aar-ρΗ3ΗβΗΗΗ nOflBHXHOft φε3Η npOUYKTaMH peaKflKH.

Ha nepTewe H3o6paxeHa KaMepa nna KpyroBoft χροΜβτοΓρεφΗΗ, BHfl.

KaMepa ΗΜββτ V-1 oSpasnyio KioBeTy H3 φτοροππβςτβ, 3aKnioueHHyio b MeTannn-necKuft Kopnyc 2 h xpoMaTorpa <£> HuecKyro nnacTHHy 3. B κιοββτβ HMeeTca nonocTb4 KanonnapHoro ceneHHa, coo6iqaiomaaca c KonbueBbíM pesepeyapoM fifth IJeHTpanbHbiňKanHnnapHbift Kahan 6 cnyatHT she noflanH pacTBopHTena b ueHTp nnacTHHbí , and 6oko * -Boft KannnnapHbift KaHan 7, peeepByap 5h nonocTb 4 KanHnnapHoro ceaeHHH - nnanoflaMH no / íBHJKHoň <J) a3bi κ πβρπφβρΗΗ nnacTHHbí. Ana npnwaTHa nnacTHHbi κ κιοΒβτβcnyxHT KpbmiKa 8 c okhom nna HaSnwneHHH 3a npoueccoM χροΜβτοΓρβφκηβοκοΓΟ aHa-nnsa. AeTann κιοΒβτω 1 HsroTOBnawTca H3 φτοροηπβοτβ.

KaMepa paOoTaeT cnenyiomHM oOpaaoM. ilpH Χρ0Μ3Τ0Γρ3φκρ0Β8ΗΗΗ; nnacTHHbí MeTOROM KOnbUeBblX Η paflHanbHMX 30H-night they HaHeceHHa npo6u Ha nnacTHHy OH and ycTaHaBnHBaeTca bhhs cnoeM b KtoeeTy 1.ΒοκοΒοή KanHnnapHbift Kahan 7 noflBoflHTca κ cnoio copSeHTa, BKnionaeTca HacocHoeycTpoňcTBO, κοτοροβ núflaeT pacTBop noflBHXHoň φβ3ω κ cnoio copSeHTa. HocneflocTHHteHHa pacTBopHTeneM KpaeB nnacTHHbí Hacocnoe ycTpoftcTBO BMKmonaeTca, nnacTHHa H3BneKaeTca KaMepbi H3 H3 H npoHBnaeTca oahhm H3BecTHbix cnocofioB (onpbicKHBaHHeM xHMHnecKHMH peareHTaMH hjih npocMaTpHBaeTca b ΥΦ-C ~). Πρκ: ΧρθΜ3ΤΟΓρ3φΗρθΒ3ΗΗΗ 'nnacTHHbí UeHTpOCTpeMHTenbHblM MeTOflOM CHHMaeTcaKonbneBaa 3OHa what cnoa copĎeHTa c Kpaa nnacTHHbí 3h HaHocHTca npo6a no nepn-φ $ ρΗΗ OKpyxHocTH, aaHHTotó cnoeM cop6eHTa. Hocne stoto nnacTHHa ycTaHaBnHBa-eTca b KaMepy cnoeM bhhs, noRXHMaeTca 8 H BKraonaeTca Hacocnoe ycT-poboTBO, κοτοροβ nepe3 βοκοΒοή KanHnnapHBI KaHan 7, pesepByap 5 h nonocTb 4 258420

KanHJHiHpHoro ceqeHHH ποαβοαητ ποαβηχηυιο φβ3γ κ cjioio cop6eHTa. Ilocne AOCTuxe-HHH ΠΟβΒΗΧΗΟή OHβ3θή AOHTpa nnacTHHbl Iipoiiecc XpOMaTOrpa $) HpOBaHHHi aaKaHUH-BarnT, nnacTHHy BbíHHMaioT on Kavep n ηροηίΜΗΐοτ oahhm H3 H3BecTHbix cnocoSoe. ΠρΗ ΧρθΜ3ΤΟΓρ3φΗρθΒ3ΗΗΗ lUiaCTHHbl ΟβΗΟΒρβΜβΗΗΟ ΜβΤΟΑΟΜ paAHanbHbIX 3 OH ΜAeHTpOCTpeMMTeJIbHblM ΜβΤΟΑΟΜ npo6bl B ΒΗΑβ ΤΟΗβΚ HaHOCHTCH Ha paCCTOHHHH ΙΟ-Ι 5mm OT ueHTpa nnacTHHbl h no oxpyxHOCTH Ha paccTOHHHH 10-15 mm xpannnacTHHw rev. nocne 3τογο nnacTHHa ycTaHaBnuBaeTCH b xaMepy cnoeM bhhs, ποαμη-MaeTca xpbunxoň 8h BKjnonaeTCH HacocHoe ycTpoňcTBO, κοτοροβ ηβρβ3 yeHTpanb-Hbiň čoxoBoň xanHnnHpHbie xaHanbi h 6 h 7 ποαβοαητ ποαβηχηυιο φa3y κ cnoio cop- .6eHTa b ueHTp nnacTHHbl no xpaHM h. Ilocne BCTpeqH Φροητοβ pacTBopHTeneennacTHHbl BblHHMaiOT H3 KaMepbl H ΠρΟΗΒΠΗΙΟΤ OflHHM H3 H3BeCTHbIX CnOCO6OB.

IlajiHMHe b xaMepe yononHHTenbHoň xonbneBoň hoaocth xanHJinHpHoro ceneHHH, HaXOAHtUeílCH C ΟΡ, ΗΟϋ CTOpOHbl B ΚΟΗΤβΚΤβ CO CJIOeM COpOeHTa ΧρΟΜβΤΟΓρβφΗΗβΟΚΟήnnacTHHbl ac Apyroň ctopohm coo6ma »MENCH c xonbneBbíM pe3epByapoM CB" 3a-HblM C HaCOCHUM yCTpoňCTBOM, ΠΟ3ΒΟΠΗβΤ OcymeCTBJÍHTb B HSBeCTHOň ΚβΜβρβ XPO "ΜβΤΟΓρβφΗΙΟ ΜβΤΟΑΟΜ KOJIbyeBUX H paAHaAbHbIX 3OH, UeHTpOCTpeMHTenbHblM ΜβΤΟΑΟΜ, and Taxxe ΟΑΗΟΒρβΜβΗΗΟ ΜβΤΟΑΟΜ paAHanbHbIX 3OH H UeHTpOCTpeMHTenbHblM ΜβΤΟΑΟΜ.

KpOMe ΤΟΓΟ O ΚΤ T ΚΤ ΚΤ ΚΤ ΒΗΧΗΟ ΚΤ ΚΤ Μ Μ Μ Τ Τ ΗΊ noc Α noc noc noc noc T T T T T T ue ue ue ue ue ue ue ue ue ue ue

ΦΟΡΜΥΠΑ H3OEPETEHHH

KaMepa απη xpyroBoň χροΜβτοΓρβφΗΗ, coAepxaman κκίΒβτγ, pacnonoxeHHyro bKOAbueBOM xopnyce c xpuniKoň χροΜβτοΓρβφΗΗβεκγιο nnacTMHy h ycTpořtcTBO ποαβηηnoABHiKHoň φβ3Μ κ cjiom copCeHTa c neHTpajibHbíM h Sokobum xaííHJinHpHbíMH xaHana-MH OTJiHMaioinaHCH τβΜ, Μτο c nejibio pacuiHpeHHH oSnaCTH ΠρΗΜβΗβ-ΗΗΗ h ynymneHHH xanecTBa ΠρΟΒβΑβΗΗΗ χροΜβτοΓρβφΗΗβεκοΓΟ aHajiH3a, KaMepacHaCxeHa xonbueebíM Φηγυρημμ BKiiaybmieM, o6pa3ywmnM c KopnycoM nonocTb xamuť-ΠΗρΗΟΓΟ CeneHHB, XOTOpaH C OAHOň CTOpOHbl HaXOAHTCH B ΚΟΗΤβΚΤβ what cnoeM braid-OeHTa χροΜΗΤΟΓρβφΗΊβεκοή rmacTHH ac Apyroň cTopoHbí coeAHHeHá c xonbueBbíMpe3epeyapoM ueHTpanhHMM xanujinapubíM by the burner κοτορωή CBH3an c xonbueeoň no-nocTbio NEP ycTpoňcTBO noyaqH ποαβηχηοΗ φβ3Μ, πρκ stom xanHJUiHpHbie xaHanbiKOJibyeBoro peeepeyapa oOpasyioT perynHTOp nepeMemeHWH anioeHTa.

Φ P E E P T A KAMEPA flJW KPyrOBOtt ΧΡΟΜΑΤΟΓΡΑΦΗΗ Η3θ6ρβΤβΗΗβ OTHOCHTCH K aHanHTHMeCKOň XHMHH B HaCTHOCTH Κ ΧρΟΜβΤΟΓρβφΗ-qecxoMy auanusy, η Μοχβτ 6μτβ HcnonbďOBaHo απη χροΜβτοτρβφΗροΒβΗΗΗ τοηκο-CAOňHblX nnaCTHH ΜβΤΟΑΟΜ XpyroBoň ΧρΟΜβΤΟΓρβφΗΗ.

Uejlb Η3θ6ρβΤβΗΗΗ - ρβΟΠΗρβΗΗβ ΟβπβΟΤΗ ΠρΗΜβΗβγ γπγΊΙΠβΗΗβ ΗβΗββββρΟΒβΑβΗΗΗ ΧρΟΜβΤΟΓρβφΗΗβΟΚΟΓΟ and HajIHSa.

KaMepa απη xpyroBoň χροΜβτοΓρβφΗΗ coyepxnT κιοΒβτγ 1 pacnonoxeHHyio β .KOjibyeBOM xopnyce 2c xpbnnxoň 8 χροΜβτοΓρβφΗπβοκγ »nnacTHHy 3 η-ycTpoňcT ΒΟ ΠΟΑβΗΗ ΠΟΑΒΗΧΗΟή φ33Μ Κ CJIOIO COpOeHTa AeHTpanbHblM C Η ÓOKOBblM ΚΗΠΗΠΠΗρ HbiMH KaHanaMH 6-η seventh KaMepa cHa6xeHa xonbueBbíM Φηγυρημμ BxnaAbímeM, xoTopuňoCpaayeT c KopnycoM 2 nonocTb 4 KanHJWHpHoro ceueHHH, κοτοράΗ c ÓAHoň cto-pOHb! HaXOAHTCH Β ΚΤβΚΤβ CO CJIOeM COpOeHTa ΧρΟΜβΤΟΓρβφΗΊβΟΚΟΗ nnacTHHbl 3, and c ň ň co e x x x x x on on on on on ea u u u

ConpoBOXAaioiitHň qepTex. 258420 4 ΠρΗ3Η3κο Η3θ6ρβτβΗΗβΜ no peaynbTaTaM SKcnepTHsw, ocymecTBJíeHHoft FocyflapcTΒβΗΗΜΜ ΚΟΜΗΤβΤΟΜ CCCP ΠΟ ΑβΠβΜ Η3θ6ρβΤβΗΗή Η OTKpbJTHň. I ΜβρΤβΧ 258420

Claims (1)

BACKGROUND OF THE INVENTION A circular chromatography chamber comprising a cuvette disposed on an annular capped body, a chromatograph and a moving phase delivery device to a sorbent layer and a central and lateral capillary passage, is a chamber provided with an annular shaped insert forming with the body (2) a capillary cavity (4) which is in contact with the sorbent layer of the chromatographic plate (3) on one side and is connected to the annular well (5) by the central capillary channel (6) from the opposite side , which is connected to the annular cavity of the mobile phase feed device, wherein the capillary channels of the annular well form a regulator for the overflow of the eluent. 258420