CS258921B1 - A method for increasing the germination of orchid seeds - Google Patents

Abstract

The method relates to increasing the germination of orchid seeds during symbiotic sowing using 4-acetamidosalicylic acid. This acid or its salt is added to the soil for asymbiotic sowing so that its concentration in the soil is 10"^ to 10-2 g/1. At this concentration, it significantly increases the germination of mature orchid seeds and in some cases even enables the germination of those species that otherwise do not germinate during asymbiotic sowing.

Description

The invention relates to a method for increasing the germination of orchid seeds.

Seed germination and further development of ORCHIDACEAE seedlings in nature is dependent on the presence of suitable mycorrhitic fungi. In the artificial cultivation of orchids, the so-called asymbiotic method is mainly used in that the sterilized orchid seeds are sown on sterile agar soils containing mineral salts, sugars or other organic substances. Many orchids germinate and develop successfully to seedlings of a size that are capable of being transferred to a non-sterile substrate. Seeds of many species, however, germinate very difficult or not at all. This is partly attributable to the existence of an embryo protective coating that prevents premature germination of the seed at inappropriate times or conditions. The protective coating may be disrupted or removed by the action of some means used to sterilize the seeds, for example sodium or calcium hypochlorite. However, even after that, the seeds of some species do not germinate or germinate only if vitamins or other factors are present in the soil, supplied to the soil as such or with natural components such as coconut milk, banana pulp and the like.

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These drawbacks are overcome by the method of increasing the germination of orchid seeds according to the invention. Its essence is that it is added to the soil for asymbiotic sowing of orchids

5 - 2 4-acetamidosalicylic acid or a salt thereof at a concentration of 10 to 10 g per liter of soil.

The main advantage of the method according to the invention is that it increases the percentage of germination of the orchid seeds in asymbiotic sowing and, in some cases, allows germination of those species whose mature seeds hardly germinate. A further advantage is that 4-acetamidamidicylic acid is a relatively accessible compound and as a chemical individual it can be unambiguously reproducibly dosed unlike natural components, the effectiveness of which usually depends on the method of preparation, conditions and storage time and possibly other factors.

The process according to the invention is further described in several examples.

Example 1

The mature seeds of Orchis morio L. were sterilized for 20 min. by shaking with 1 ?. aqueous NaClO solution and sown on soil (g / l) 0.216 K1 PO4, 0.245 MgSO4, 7H./), 0.150 KCl, 0.132 (NH4SO4), 0.022 CaCl ₇ , 0.192 citric acid, 0.5 yeast autolysate, 0.5 peptone, 10.0 glucose, 8.0 agar whose pH was adjusted to 5.2 with 1M-KOH The sowing was stored in trios at 15 to 20 ° C. 140 days after sowing no no signs of germination observed.

Example 2 mature Orchis morio L seeds were sterilized and sown in the same manner as in the example and on the same soil to which 4-acetamidosylic acid was added (hereinafter -4).

AcPAS) so that the concentration of this compound in the soil is 1.10 g / l. Approximately 20% of the seeds germinated 140 days after sowing and the seedlings formed leaves 5-10 mm long.

Example 3

The mature Orchis morio L seeds were sterilized and sown in the same manner as in Example 1 and on the same soil to which AcPAS sodium was added so that the concentration of this compound in the soil was 1.10 ¹ g / L. 140 days after sowing, 30% of the seeds germinated and the seedlings formed leaves 5-10 mm in length.

Example 4

Mature seeds Orchis morio L. was sterilized and sown in the same manner as in Example 1 on the same soil, to which was added sodium AcPAS so that the concentration of the compound in the soil was 10.1 ¹ g / 1st After 60 days the first signs of germination were observed, after 140 days the germinated protocorms were dead.

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Example 5

The mature seeds of Cypripedium calceolus L. were sterilized with 40 nir. by shaking with 31 aqueous NaClO solution and sown on soil of the same composition as in Example 1. The sowing was stored: »in the dark at 15 to 20 ° C. No signs of germination were observed 200 days after sowing

Example 6

The mature seeds of Cypripedium calceolus L. were sterilized and sown in the same manner as in Example 5 onto the soil of Example 1, to which AcPAS sodium was added so that its concentration in the soil was 1.101.10 g / l. After 200 days after sowing, it germinated approximately from% of seeds and protocorms reached the size of 1 to 2 mm.

Example 7

The mature seeds of Cypropedium calceolus L. were sterilized and sown in the same manner as in Example 5 to the soil of Example 1, to which AcPAS sodium was added to a concentration of 1.10 g / l in the soil. After 200 days sowing germinated about 10 ^f 0 seeds and protocorms reached the size of 1 to 2 mm.

Claims (1)

A method for increasing the germination capacity of an orchid seed, comprising adding acid to the asymbiotic seed of orchids. 4-acetaraidosalicyl or a salt thereof in conc