CS260896B1 - Filling reactor for micro-column high-efficient liquid chromatography - Google Patents
Filling reactor for micro-column high-efficient liquid chromatography Download PDFInfo
- Publication number
- CS260896B1 CS260896B1 CS867965A CS796586A CS260896B1 CS 260896 B1 CS260896 B1 CS 260896B1 CS 867965 A CS867965 A CS 867965A CS 796586 A CS796586 A CS 796586A CS 260896 B1 CS260896 B1 CS 260896B1
- Authority
- CS
- Czechoslovakia
- Prior art keywords
- liquid chromatography
- reactor
- micro
- efficient liquid
- column high
- Prior art date
Links
- 238000004811 liquid chromatography Methods 0.000 title 1
- 239000000835 fiber Substances 0.000 claims description 4
- 239000011152 fibreglass Substances 0.000 claims description 4
- -1 polytetrafluoroethylene Polymers 0.000 claims description 4
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 4
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 4
- 238000004128 high performance liquid chromatography Methods 0.000 claims description 3
- 239000011324 bead Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000000571 coke Substances 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Physical Or Chemical Processes And Apparatus (AREA)
Description
Předmětem vynálezu je náplňový reaktor pro mikrokolonovou vysokoúčinnou kapalinovou chromatografii naplněný vlákny ze skelného uhlíku, který se řadí za chromatograflckou kolonu.SUMMARY OF THE INVENTION The present invention provides a packed reactor for microcolumn high performance liquid chromatography filled with fiberglass fibers downstream of a chromatography column.
Pro realizaci rychlých pokolonových re-, akcí v mikrokolonové vysokoúčinné kapalinové chromatografii jsou užívány dva typy reaktorů: Reaktor kapilární — většinou spirálově stočená kapilára o 0 cca 0,05-+-0,1 milimetru — a reaktory náplňové — o 0 cca 1 mm naplněné nejčastěji skleněnými kuličkami o 0 cca 5 μΐη. Nevýhodou kapilárních reaktorů je velký tlakový spád. Nevýhodou náplňových je malá chemická odolnost dostupných skleněných kuliček v alkalické oblasti.Two types of reactors are used for realization of fast post-column reactions in microcolumn high-performance liquid chromatography: Capillary reactor - mostly spiral coiled capillary 0 0 0.05 - + - 0.1 millimeter - and packed reactors - 0 0 mm filled most often with glass beads of 0 approx. 5 μΐη. The disadvantage of capillary reactors is the large pressure drop. The disadvantage of the cartridges is the low chemical resistance of the available glass beads in the alkaline region.
Uvedené nedostatky částečně řeší náplňový reaktor podle vynálezu, jehož podstatou je, že prostor reaktoru je vytvořen vý96 vrtem v pouzdru vyrobeném z plněného polytetrafluorethylenu a vyplněném podélně orientovanými vlákny skelného uhlíku o průměru menším než 15 μηι a větším než 1 μΐη.These drawbacks are partially solved by a packed reactor according to the invention, which is based on the fact that the reactor space is formed by a borehole in a housing made of filled polytetrafluoroethylene and filled with longitudinally oriented fiberglass fibers of less than 15 μηι and greater than 1 μΐη.
Na připojeném výkresu je schematicky znázorněn realizovaný příklad reaktoru.The attached drawing shows schematically an exemplary reactor.
Těleso reaktoru 1 se sroubeními 2 pro přívodní kapiláry je opatřeno pouzdrem 3 z polytetrafluorethylenu s 25 °/o koksu. V pouzdru 3 je vývrt 4 o průměru 1 mm, naplněný podélně orientovanými vlákny 5 skelného uhlíku o 0 cca 4 μΐη. Prostor vývrtu 4 je uzavřen sítky 6 z nerezavějící oceli.The reactor body 1 with the inlet capillary fittings 2 is provided with a sleeve 3 of 25% polytetrafluoroethylene with coke. In the housing 3, a bore 4 of 1 mm diameter is filled with longitudinally oriented fiberglass fibers 5 of about 4 μΐη. The space of the bore 4 is closed by stainless steel screens 6.
Reaktor 1, ve kterém jsou mobilní fází smáčeny pouze součástky vyrobené z polytetrafluorethylenu, vlákna 5 skelného uhlíku a přívodní kapiláry, způsobuje při průtoku cca 40 μΐη/ιηϊη. objemové rozmytí cca 1 μΐ a je výrobně jednoduchý.Reactor 1, in which only the components made of polytetrafluoroethylene, glass fiber 5 and the feed capillary, is wetted by the mobile phase, causes a flow rate of about 40 μΐη / ιηϊη. 1 µΐ volume sweep and is simple to manufacture.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS867965A CS260896B1 (en) | 1986-11-04 | 1986-11-04 | Filling reactor for micro-column high-efficient liquid chromatography |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS867965A CS260896B1 (en) | 1986-11-04 | 1986-11-04 | Filling reactor for micro-column high-efficient liquid chromatography |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CS796586A1 CS796586A1 (en) | 1988-06-15 |
| CS260896B1 true CS260896B1 (en) | 1989-01-12 |
Family
ID=5429676
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CS867965A CS260896B1 (en) | 1986-11-04 | 1986-11-04 | Filling reactor for micro-column high-efficient liquid chromatography |
Country Status (1)
| Country | Link |
|---|---|
| CS (1) | CS260896B1 (en) |
-
1986
- 1986-11-04 CS CS867965A patent/CS260896B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CS796586A1 (en) | 1988-06-15 |
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