CS261100B1 - J Microorganism strain Lactobacillus cellobiosus CCM 4 000 - Google Patents

Abstract

The solution relates to a strain of the microorganism Lactobacillus cellobiosus CCM 4,000, which produces biomass with adherence to epithelial cells of the rumen wall and with stable production of alpha amylase activity and ethanol. The strain produces biomass that has use as a colonization preparation for young ruminants.

Description

The present invention relates to a strain of Lactobacillus cellobiosus CCM 4,000 which produces biomass with adherent epithelial cells of the rumen wall and stable production of alpha amylase activity and ethanol. The strain produces biomass which is used as a colonization preparation of ruminant babies. 261100 4 261100

The present invention relates to a novel strain of Lectobacillus cellobiosus CCM 4,000 producing biomass and ethanol with adherent epithelial cells of the rumen wall and with stable amylase activity, which is particularly useful as a colonization preparation for young ruminants.

Lactobacillus strains used as microbiotics such as Lactobacillus plantarum CCM 1834 and Lactobacillus lactis CCM 1877 were not selected to be used to stimulate the development of rumen mikoilora in ruminant pups, i.e., adherence, stable alpha amylase and ethanol production as ruminatorium.

The aforementioned drawbacks of the aforementioned known strains are eliminated by the invention, which is a novel strain of Lactobacillus cellobiosus microorganisms deposited in the Czechoslovak Collection of Microarganism of the Jan Evangelist Purkyně University, Tř. Obránců míru 10 in Brno under CCM 4 000.

The Lactobacillus cellobiosus strain CCM 4,000 was obtained by long-term culture passage of the Lactobacillus cellobiosus strain isolated from the rumen content of the calf, in the presence of the elimination plasmid e.g. mto1 acridine orange in nutrient medium according to the Certificate of Approval No. 248 207 entitled "Method of Choosing Rumen Microorganisms". for the preparation of colonization preparations ". (Kmet V. et al. 1986).

The procedure is given in Example 1.

The new strain of microorganism has the following morphological and physiological properties: Gram-positive immobile rods. Rogosa agar (Oxoid) is a medium-sized creamy white 2-3 mm large colony. Anaerobically hydrolyses starch. Ferments ferment arabinose, celophaiosis, altosis. It has acid protease activity and produces 0.5 moles ethanol. mole-1 of utilized glucose.

The method of producing a colonization preparation using the novel strain is described in Example 2 for illustration. Example 1

A new strain of Lactobacillus cellobiosus CCM 4,000 was obtained after multiple passage cultivation of the flood isolate selected for amylase activity and ethanol production. Acridine orange added to the Rogosa medium (Example 2) at 0.5 µg. ml-1 media, i.e., at the subinhibitory concentration, caused accelerated elimination of any unstable plasmids encoded by the properties, making the new strain genetically stable. Example 2

The culture of Lactobacillus cellobiosus CCM 4000, 36 hours old, grown on Rogosa agar with 1% corn starch, is seeded with inoculum broth of the following composition in g. 1: peptone 10, yeast extract 5, glucose 20, polyoxyethylene sorbitan monooleate (Tween 80) 1, magnesium sulfate 0.2, manganese sulfate 0.05, pH 6.0, sterilization for 30 minutes at 120 ° C and incubated at 37 ° C. ° C for 10 to 12 hours. A 10-liter fermenter, filled with 8,000 ml of medium of the same composition, is seeded in the inoculum at 5% by volume. After 8- to 10-hour anaerobic incubation at 37 ° C, stirring for 300 to 500 revolutions. min.-1, the biomass obtained is concentrated and dried. The prepared colonization preparation is mixed into any mixed mixture for calves, respectively. for lambs so that the average daily dose of Lactobacillus cellohiosu strain CCM 4,000 is 1.10 µg for calves, respectively. 5,108 germs for lambs. The preparation is given during the period of the milk diet.

Claims (1)

SUBJECT A strain of Lactobacillus cellobiosus CCM 4,000 producing biomass with epithelial cell rumen-adherence cell adherence and stable alpha and mylase activity and ethanol production.