CS264505B1 - Xanthine reductase inhibiting agent - Google Patents

Xanthine reductase inhibiting agent Download PDF

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Publication number
CS264505B1
CS264505B1 CS841902A CS190284A CS264505B1 CS 264505 B1 CS264505 B1 CS 264505B1 CS 841902 A CS841902 A CS 841902A CS 190284 A CS190284 A CS 190284A CS 264505 B1 CS264505 B1 CS 264505B1
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CS
Czechoslovakia
Prior art keywords
xanthine
inhibiting agent
reductase
reductase inhibiting
acetyl
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CS841902A
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Czech (cs)
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CS190284A1 (en
Inventor
Cestmir Mudr Csc Kalina
Jiri Prom Biol Ing Drsc Ponert
Miloslava Potacova
Jiri Prof Ing Drsc Jary
Original Assignee
Cestmir Mudr Csc Kalina
Jiri Prom Biol Ing Drsc Ponert
Miloslava Potacova
Jary Jiri
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Application filed by Cestmir Mudr Csc Kalina, Jiri Prom Biol Ing Drsc Ponert, Miloslava Potacova, Jary Jiri filed Critical Cestmir Mudr Csc Kalina
Priority to CS841902A priority Critical patent/CS264505B1/en
Publication of CS190284A1 publication Critical patent/CS190284A1/en
Publication of CS264505B1 publication Critical patent/CS264505B1/en

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Abstract

Prostředek inhibující xanthin-reduktázy je tvořen sloučeninou kyseliny N-acetyl-p-aminosalicylové vzorce coox ■OH o. NHCOCH3 kde X je anorganický nebo organický kation. Prostředek může být využit v lékařské diagnostice, onkologii a biotechnologii a v genovém inženýrství.The xanthine reductase inhibiting agent is formed by the compound N-acetyl-p-aminosalicylic acid of the formula coox ■OH o. NHCOCH3 where X is an inorganic or organic cation. The agent can be used in medical diagnostics, oncology and biotechnology and in genetic engineering.

Description

Vynález se týká prostředku inhibujícího xanthinreduktázy.The present invention relates to a xanthine reductase inhibitor composition.

Xanthin-O2-reduktáza (známá též pod starším názvem xanthin-oxidáza) se vyskytuje ve sliznici lidského střeva a v lidských játrech. Metoda stanovení její aktivity je popsána v knize Jakoby W. B. (ed.): Enzyme Purification and Related Techniques, vol. 22, p. 150—152, Academie Press New York and London 1971.Xanthin-O 2 -reductase (also known as the xanthine oxidase) occurs in the human mucosa and the human liver. A method of determining its activity is described in Jakoby WB (ed.): Enzyme Purification and Related Techniques, vol. 22, pp. 150-152, Academic Press New York and London 1971.

Sodná sůl kyseliny N-acetyl-para-aminosalicylové získala státní registraci SSSR No. 388 281 (chemické sloučeniny s biologickým účinkem).N-Acetyl-para-aminosalicylic acid sodium salt obtained US State Registration No. 388 281 (chemical compounds with biological activity).

Jak vyplývá z následujícího příkladu, použití sloučeniny vzorceAs follows from the following example, the use of a compound of formula

COONaCOONa

NHCOCH3 je založeno na objevení její nové vlastnosti, a to jejího inhibičního účinku na xantin-reduktázy, při současné její netoxičnosti. Podstatou vynálezu je prostředek inhibující xanthin-reduktázu, který je tvořen sloučeninou vzorce coox é?°H kde X je anorganický nebo organický kation.NHCOCH3 is based on the discovery of its new property, its xanthine reductase inhibitory activity, while at the same time being non-toxic. The present invention is directed to a xanthine reductase inhibiting composition comprising a compound of the formula coox 6 wherein H is an inorganic or organic cation.

Vynález může být využit v lékařské diagnostice a v onkologii. Rovněž je perspektivní v oblasti biotechnologie a genovém inženýrství.The invention can be used in medical diagnostics and oncology. It is also promising in the field of biotechnology and genetic engineering.

Prostředek podle vynálezu se objasňuje v dále uvedeném příkladu:The composition according to the invention is illustrated in the following example:

U sodné soli kyseliny N-acetyl-para-aminosalicylové byl autory vynálezu objeven inhibující účinek na xanthin reduktázy. Ke stanovení byla užita standardní metodika Ústavu pro biologické zkoušení chemických sloučenin SSSR.The sodium salt of N-acetyl-para-aminosalicylic acid has been found to inhibit xanthine reductase. The standard methodology of the Institute for Biological Testing of Chemical Compounds of the USSR was used for the determination.

50% inhibice xanthin-O2 reduktázy je působena 0,8.10-4M roztokem sodné soli N-acetyl-para-aminosalicylové. 50% inhibice (I50) xanthin-DChFIF-reduktázy je dosahováno 0,8.10~4 roztokem sodné soli kyseliny N-acetyl-para-aminosalicylové (Vědecko-výzkumný ústav pro biologické zkoušení chemických sloučenin).50% inhibition of xanthine-O 2 reductase is caused by a 0.8.10 -4 M solution of N-acetyl-para-aminosalicyl sodium. 50% inhibition (I 50) xanthine DChFIF reductase is achieved 0,8.10 -4 solution of sodium salt of N-acetyl-para-aminosalicylic acid (Scientific-Research Institute for Biological testing of chemical compounds).

Přitom je Na-sůl kyseliny N-acetyl-para-aminosalicylové netoxická, o čemž svědčí následující údaje. Akutní toxicita sloučeniny ve fysiologickém roztoku byla testována intravenosně na myších — je nižší než 0,5 g/kg tělesné hmotnosti. Chronická toxicita byla testována perorálně na krysách při denních dávkách 0,5 g/kg tělesné hmotnosti podávaných po dobu 10 dnů a při denních dávkách 1 g/kg tělesné hmotnosti podávaných po dobu 10 dnů. Proti kontrolní skupině, jak v případě samic, tak v případě samců, nebyly zjištěny rozdíly, což svědčí o netoxičnosti i při vysokých denních dávkách opakovaně podávaných.The Na-salt of N-acetyl-para-aminosalicylic acid is non-toxic, as evidenced by the following data. The acute toxicity of the compound in saline was tested intravenously in mice - less than 0.5 g / kg body weight. Chronic toxicity was tested orally in rats at daily doses of 0.5 g / kg body weight administered for 10 days and at daily doses of 1 g / kg body weight administered for 10 days. There were no differences in the control group, both in females and males, suggesting non-toxicity even at high daily doses repeatedly.

Claims (1)

Prostředek inhibující xanthin — reduktázy, kde X je anorganický nebo organický kation, vyznačený tím, že je tvořen sloučeninou kyselí- například Na. ny N-acetyl-p-aminosalicylové vzorce cooxA xanthine reductase inhibitor composition wherein X is an inorganic or organic cation, characterized in that it is an acidic compound such as Na. N-acetyl-p-aminosalicyl coox
CS841902A 1984-03-16 1984-03-16 Xanthine reductase inhibiting agent CS264505B1 (en)

Priority Applications (1)

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CS841902A CS264505B1 (en) 1984-03-16 1984-03-16 Xanthine reductase inhibiting agent

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CS841902A CS264505B1 (en) 1984-03-16 1984-03-16 Xanthine reductase inhibiting agent

Publications (2)

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CS190284A1 CS190284A1 (en) 1988-09-16
CS264505B1 true CS264505B1 (en) 1989-08-14

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