CS266251B1 - The way to detect malignancies - Google Patents

Abstract

Detection of malignancies from patient blood serum is achieved by treating brewer's yeast with this serum under investigation and, for control, with blood serum from a healthy person or a 1% sodium chloride solution for 24 hours at a temperature of 35 to 40 °C, and by comparing the increase in the number of yeast in the serum under investigation and the control, the malignancy index is determined.

Description

The invention solves the method of detecting malignancies, just at the beginning of their occurrence, from a blood serum sample and thus the possibility of a correct diagnosis in the human body arises.

Until now, according to world literature, until 1983, the possibility of detecting malignant neoplasms, the possible method was too lengthy, expensive and only 60 to 65% certain.

The invention makes it possible to detect a malignant tumor in the human body, within 24 hours, by a method which is rapid, fasting and is based on a percentage of 98%.

The invention is based on the properties of yeast cells. Brewer's yeast, Sacharomycaes cerevisiae, is very sensitive to foreign-internal environments. They have either an imperceptible or coarse-grained structure that passes through the coarse-grained, only when they are good old. They contain larger amounts of vacuoles. After staining with Lugel's solution, they appeared young fresh, few of which were solid brown. These are resting yeasts. The old yeast was stained blue with Lugol in pH 4.6 buffer. In nature, Sacharomycaes cerevisiae are distinguished from cultural ones by the fact that when cultivated under the same conditions, they culturally form strains, bottom fermentation, spores, later than wild yeasts.

years, malignancies have been detected by yeast cells, i.e. by a yeast reaction. Patient sera were collected from surgery, gynecology, internal medicine. Yeast after 24 hours in the thermostat appeared in a larger number than dead yeast, also in a smaller number compared to the control. From the beginning, only those sera were taken that had a small clinical diagnosis of t. j. malignancy. Later, positive results appeared even where the diagnosis was first guessed, where it was not verified, until later, after a year when metastases had already occurred. During this examination, it was considered necessary to dilute the solution carefully to avoid a lot of accumulated yeast in the solution. So that they can be calculated in the test, also in the blank. It is stated and microscopically visible that in each attempt to detect a decrease in the number of yeasts. When the yeast is stained, it is clear in an attempt to see more yeast dead and degenerated, although the number would be equal to the control.

Verification method - electrophoresis on paper.

Alpha globulins I 11.4 - 6.5% Alpha globulins II 23.5 - 10.6% Globulins beta 7.3 - 15.1 « Gamma globulins 12.4 - 22.2% This is how they appear in the serum patient who is biochemical examination in the norm. Electrophoresis at Ca from sere: Albumins 59.55 » Alpha globulins I 15.85% Alpha globulins II 26.9% Globulins beta 7.85 S Gamma globulins 19.85%

Methodology - laboratory technique:

Brewer's yeast is placed in an Erlenmeyer flask. The wort, also obtained in a brewed beer, is placed in an Erlenmeyer flask. These flasks are placed in a thermostat at 37 ° C. C. After blood collection from the patient, the blood is centrifuged and pooled with serum. 6.2 ml of serum is collected and placed in an Erlenmeyer flask. To this is added 2 ml. worts, 2 ml. yeast and 2 ml. 10% glucose. This solution is stirred and placed in a thermostat for 24 hours at 35-40 ° C. The same procedure is performed with the serum of a healthy patient who is not presumed to be ill. A 1% NaCl solution can also be used instead of serum. The yeast is counted after 24 hours before being placed in the thermostat in the burger chamber.

Claims (2)

OBJECT OF THE INVENTION A method for detecting malignancies from the blood serum of patients, characterized in that At the same time, the blood serum and, for control, the blood of a healthy person or a 1% sodium chloride solution, were treated for 24 hours on brewer's yeast at a temperature of 35 to 40 ° C and by comparing piiiantkii kv.i ·; i n i ok u n k ι min nób <»π ·· ιη n checks for nlnimví malignancy index. 2. The method according to item 1, characterized in that the number of yeasts is determined in a burger chamber.