CS269024B1 - A method for obtaining enzymes from fermentation broths and additional liquors from the production of 1-zinc by ultrafiltration - Google Patents

Abstract

The purpose of the solution is to extract enzymes, especially proteases, from fermentation broth or mother liquors from the production of 1-lysine by ultrafiltration. By ultrafiltration, it is possible to isolate and concentrate these enzymes from the fermentation broth or mother liquors so that they reach a concentration in which they are practically usable.

Description

The present invention provides ultra-ultraviolet enzymes which are industrially useful in fermentation soils from 1-lysine production.

Fermentation production of 1-lysine is carried out using Brevlbecterlum flavum, or Corynebacterium species, Micrococcue glutamlcus. The main carbon source in the fermentation broth is eacharose and glucose. In addition, the fall contains corn steep liquor, protein substrate hydrolyzate, ammonium salts. The culture is carried out at 29 ° C with vigorous stirring and aeration for about 20 hours. At the end of the culture and ea, the cells of the producer are aspirated by means of a suitable separator. Crystalline 1-lysine is obtained from the aupernatant and using an aeparator.

While cultivating the production strain, 1-lysine gates produce even more salivary substances including axtracellular enzymes. These were quenched in the conventional manner of isolating 1-lysine from the fermentation broth, and read into the mother liquors after crystallization of 1-lysine.

These substances can be removed from the fermentation broth after the cultivation and the removal of the producer cells by ultrafiltration through an ultrafiltration membrane and a delta of 5,000-50,000 molecular weight, which retains the high molecular weight substances and, on the contrary, releases the low molecular weight components from the fermentation broth. Optionally, these substances can be removed by ultrafiltration from the mother liquors after crystallization of 1-lysine.

From the ultra-high molecular weight concentrate obtained by ultrafiltration, I may have obtained several important enzymatically important enzymes, mainly proteases. These enzymes, which do not utilize ethane as a byproduct of the production of penicillin a and have so far not been utilized.

Example 1

000 000 parts of the fermentation broth from the production of 1-lysine, after the cells of the producer of Brevlbecterlum flavum were removed from the separator, were ultrafiltered through an ultrafiltration membrane and a 10,000 molecular weight cut-off at 20 ° C and 0.3 MPa. 990,000 parts of low molecular weight permeate are separated through the membrane and 10,000 parts of the concentrate containing 1 dl of enzymes remain above the membrane. The purified high molecular weight free pereate was used for l-lysine isolate. The concentrate of ultrafiltrate ea will be used for the production of enzymes.

Example 2

000 000 parts of mother liquors after crystallization of 1-lysine from the production of 1-lysine by the producer of Corynebacterium species, containing 50 parts of enzymes, are ultrafiltered through an ultrafiltration membrane with a deletion of 20,000 molecular weight at 50 ° C and 0.5 MPa. In this process, 900,000 parts of the low molecular weight permeate are separated across the membrane and 100,000 parts of the concentrate containing 50 parts of the enzymes remain above the membrane. The ultrafiltration ea concentrate used for the production of enzymes *

Claims (1)

OBJECTS OF THE INVENTION A method for the enrichment of enzymes by ultrafiltration of a 1-lysine fermentation process characterized in that 1,000,000 parts of a 1-lysine fermentation process after separation of cells of a 1-lysine producer of Brevlbecterlum flavum, or Corynebacterium species, or Micrococcue glutamlcus, respectively, are in the mother liquor after crystallization of lysine containing 1 to 50 parts of enzyme and ultrafiltered through a semi-wettable ultrafiltration membrane with a dividing line of 5,000 to 50,000 molecular weight at a temperature of 5 to 50 ° C at a pressure of 0.1 to 0.5 MPa, and e. 800,000 to 990,000 parts of the non-molecular weight permeate permeate, and 10,000 to 200,000 parts of a concentrate containing 1 to 50 parts of enzyme remain above the membrane.