CS270103B1 - wall / l-> 6 / -beta-o-gluco- / 1 - ► 3 / -beta-o-glucan yeast - Google Patents
wall / l-> 6 / -beta-o-gluco- / 1 - ► 3 / -beta-o-glucan yeast Download PDFInfo
- Publication number
- CS270103B1 CS270103B1 CS863944A CS394486A CS270103B1 CS 270103 B1 CS270103 B1 CS 270103B1 CS 863944 A CS863944 A CS 863944A CS 394486 A CS394486 A CS 394486A CS 270103 B1 CS270103 B1 CS 270103B1
- Authority
- CS
- Czechoslovakia
- Prior art keywords
- beta
- gluco
- wall
- glucan
- yeast
- Prior art date
Links
Landscapes
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
stěnový /l«*6/-beta-D-glukó- (l-»-3/-beta-D-glukén kvasiniek a /06/20 + 5,0 až 8,8 0 /c«0,50 v DMSO/ molekulové hmotnosl 4xl05 až 2x10®, obaah dusíka o až 0,2 % hmot. stanovený podlá Dumasa/, obsah popola 0,3 až 0,6 % hmot., Ič-spektrum v KBr 892, 1045, 1080, 1160, 1205, 1252, 1315, 1375, 1640, 2922, 3434 CID“1, 13CNMR spektrum v DMSO-dfi so signálom 86,21 ppm. Ůčelom rieSenia je stěnový /l-*6/- -beta-D-gluko-/l-»3/-beta-D-glukán s biologicky účinnou Struktúrou, chemicky a fyzikálno chemicky definovaný, ktorý má použitie ako adjuvantná zložka vakcin a ako základna látka pre přípravu vo voda rozpustných stimulátorov nespecifických imunitných mechanizmov.wall /l«*6/-beta-D-gluco- (l-»-3/-beta-D-glucan of yeast and /06/20 + 5.0 to 8.8 0 /c«0.50 in DMSO/ molecular weightl 4xl05 to 2x10®, obaah nitrogen up to 0.2% by weight determined by Dumas/, ash content 0.3 to 0.6% by weight, IR spectrum in KBr 892, 1045, 1080, 1160, 1205, 1252, 1315, 1375, 1640, 2922, 3434 CID"1, 13CNMR spectrum in DMSO-dfi with a signal of 86.21 ppm. The purpose of the solution is wall /l-*6/- -beta-D-gluco-/l-»3/-beta-D-glucan with a biologically effective structure, chemically and physicochemically defined, which has use as an adjuvant component of vaccines and as a base substance for the preparation of water-soluble stimulators of non-specific immune mechanisms.
Description
Vynález ea týká stěnového /l-*6/-beta-D-gluko-/l-»3/-beta-D-glukánu kvasiniek.The invention relates to a yeast wall (1-) 6 -beta-D-gluco- [1- (3H) -beta-D-glucan.
Stěnové /l->6/-beta-D-gluko-/l-»»3/-beta-D-glukány tvoria skeletálnu mikrofibrilárnu část buňkových stien húb. Sú nerozpustné v organických rozpúétadlách, voda a v zriedených alkáliách, z farmakologického hlediska aú to látky netoxické, ktoré nešpecificky zvyšujú rezistenciu hostitele, voči r8znym chorobným procesom najma bakteriálnym, virusovým a hubovým infekciám, imunodeficientným atavom a neoplastickým procesorom. 3avla výrazný rádioprotektívny efekt a poeobia ako adjuvanty pri imunizácii a výrobě vakcín /G. Chihara, Y. Y. Maeda, 0. Hamuro, int. 3. Tise, Reac., 4, 207, 1982: N. R. Diluzio, Trends Pharm. Sel., 4, 344, 1983/.Wall [1-> 6] -beta-D-gluco- [1-> β-beta-D-glucans form the skeletal microfibrillar part of the cell walls of fungi. They are insoluble in organic solvents, water and dilute alkalis, from a pharmacological point of view and are non-toxic substances which non-specifically increase the resistance of the host to various disease processes, in particular bacterial, viral and fungal infections, immunodeficiency and neoplastic processors. 3avla significant radioprotective effect and poeobia as adjuvants in immunization and vaccine production / G. Chihara, Y. Y. Maeda, 0. Hamuro, int. 3. Tise, Reac., 4, 207, 1982: N. R. Diluzio, Trends Pharm. Sel., 4, 344, 1983 /.
Podstatou vynálezu je stěnový /l->6/-beta-D-gluko-/l->3/-beta-D-glukán kvasiniek s biologicky účinnou Struktúrou obecného vzorce beta-D-GlcpThe subject of the invention is a wall (1-> 6) -beta-D-gluco- (1-> 3) -beta-D-glucan of yeast with a biologically active structure of the general formula beta-D-Glcp
A-*3/-beta-D-Glcp/ /l-»3/-beta-D-Glcp)^7jQA- * 3 / -beta-D-Glcp / / l- »3 / -beta-D-Glcp) ^ 7jQ
220-1100 kde Glcp je glukopyrenozylová jednotka.220-1100 where Glcp is a glucopyrenosyl unit.
Stěnový /i-»6/-beta-D-gluko-/l~*3/-beta-D-glukán kvasiniek sa získá frakcionáciou buňkových stíén postupem podlá 0. 0. Bella a 0. H. Northcota, 3. chem. Soc. 1950, 1944 a jeho modifikáclami A. L.Houwink, 0. R. Kreger, Antonie van Leeuwenhoek 19, 1, 1953) S. Peat, W. 3. Whelan, T. E. Edwards, 3. Chem. Soc. 1958, 3862) 3. S. D. Bacon, W. C. Farmer, D. 3ones, 3. F. T. Taylor, Biochem. 3. 114, 557, 1969) D. 3. Manners, A. 3. Masson, 3. C. Patterson, 3. Gen. Microbiol. 80, 411, 1974) 3. Jelsma, D. R. Kreger, carbohyd. Res. 43, 200, 1975.Yeast wall (i-> 6) -beta-D-gluco- (1 * 3) -beta-D-glucan is obtained by fractionating cell shadows according to the procedure of 0. Bella and 0. H. Northcota, 3. chem. Soc. 1950, 1944 and its modifications A. L. Howink, 0. R. Kreger, Antonie van Leeuwenhoek 19, 1, 1953) S. Peat, W. 3. Whelan, T. E. Edwards, 3. Chem. Soc. 1958, 3862) 3. S. D. Bacon, W. C. Farmer, D. 3ones, 3. F. T. Taylor, Biochem. 3. 114, 557, 1969) D. 3. Manners, A. 3. Masson, 3. C. Patterson, 3. Gen. Microbiol. 80, 411, 1974) 3. Jelsma, D. R. Kreger, carbohyd. Res. 43, 200, 1975.
Příklad 1Example 1
100 g pekárenského droždla Saccharomyces cerevisiae sa suspenduje v 150 ml 6 % hmot, hydroxidu sodného a pri 60 °C sa mleša 4 h. suspenzia sa zriedi 100 ml 60 °C teplej vody a odstředí, sediment sa suspenduje v 150 ml 3 % hmot, hydroxidu sodného, mieša pri 80 až 100 °C 2 h a odstředí, sediment sa třikrát premyje vodou a extrahuje třikrát 20 ml 4 % hmot, kyselinou chlorovodíkovou alebo kyselinou fosforečnou za miešania pri teplote mieatnosti 2 h a odstředí, sediment sa premýva vodou dovtedy až supernatant je číry a reaguje neutrálně. Výtažok 4,5 až 5,4 % hmot, počítané na suéinu kvasiniek. Mol. hmotnost: 4 x 10S až 2 x 1O6. Glukán neobsahuje dusík a obsah popola je 0,5 % hmot. Infračervené spektrum: 892, 1045, 1080, 1160, 1205, 1252, 1315, 1375, 1640, 2922, 3434 cm1.100 g of baker's yeast Saccharomyces cerevisiae are suspended in 150 ml of 6% by weight sodium hydroxide and stirred at 60 ° C for 4 hours. The suspension is diluted with 100 ml of 60 ° C warm water and centrifuged, the sediment is suspended in 150 ml of 3% by weight. sodium hydroxide, stirred at 80-100 ° C for 2 h and centrifuged, the sediment was washed three times with water and extracted three times with 20 ml of 4% w / w, hydrochloric acid or phosphoric acid with stirring at room temperature for 2 h and centrifuged, the sediment was washed with water until the supernatant was clear and reacts neutrally. Yield 4.5 to 5.4% by weight, based on the yeast dry matter. Moth. weight: 4 x 10 S to 2 x 10 6 . Glucan does not contain nitrogen and the ash content is 0.5% by weight. Infrared Spectrum: 892, 1045, 1080, 1160, 1205, 1252, 1315, 1375, 1640, 2922, 3434 cm -1 .
Modifikovaný spdsob příkladu 1 s použitím org* kyselin a enzýmov sa uskutočni podlá známého’postupu D. 3. Manners, A. 3. Masson, 3. C. Patterson, Biochem. 3. 135, 19, 1973. .The modified method of Example 1 using org * acids and enzymes was performed according to the known procedure D. 3. Manners, A. 3. Masson, 3. C. Patterson, Biochem. 3. 135, 19, 1973.
příklad 2 ' .Example 2 '.
Perítoneálne makrofágy kontrolných morčiat /namieato stenového/l->6/-beta-D-gluko-/l-*3/-beta-D-glukánu dostali fyziologický roztok/ malí stupeň metabolickej aktivity 8,1 femtomSlov redukovaného formazánu, fagocitárnu aktivitu 64,8 % a za hodinu bolí schopné usmrtit 35,9 % přítomných patogénnych kandíd. Naproti tomu perítoneálne makrofágy morčiat, ktoré dostali 3 dávky stěnového /l->6/-beta-D-gluko-/l-*>3/-beta-D-glukánu v dňoch 12, 7, 3 dni před odberom makrofágov v množatve 10 mg/kg žívej váhy,Peritoneal macrophages of control guinea pigs (wall> /> 6) -beta-D-gluco- (1- * 3) -beta-D-glucan received saline / low metabolic activity 8.1 femtomSlov reduced formazan, phagocytic activity 64 .8% and per hour were able to kill 35.9% of the pathogenic candidates present. In contrast, peritoneal macrophages of guinea pigs that received 3 doses of wall / l-> 6 / -beta-D-gluco- / 1 - *> 3 / -beta-D-glucan on days 12, 7, 3 days before collection of macrophages in the amount 10 mg / kg body weight,
CS 270103 Bl tieto makrofágy mail stupeň metabolickej aktivity stimulovaný na 13,5 fentomólu redukovaného formazánu, rovnako bola zvýšená fagocitárna aktivita priemerne na 72,9 % a schopnost usmrcovat patogénne kandidy na 58,2CS 270103 B1 these macrophages mail the degree of metabolic activity stimulated to 13.5 phentomole of reduced formazan, as well as increased phagocytic activity increased on average to 72.9% and the ability to kill pathogenic candidiasis to 58.2
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS863944A CS270103B1 (en) | 1986-05-29 | 1986-05-29 | wall / l-> 6 / -beta-o-gluco- / 1 - ► 3 / -beta-o-glucan yeast |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS863944A CS270103B1 (en) | 1986-05-29 | 1986-05-29 | wall / l-> 6 / -beta-o-gluco- / 1 - ► 3 / -beta-o-glucan yeast |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CS394486A1 CS394486A1 (en) | 1989-11-14 |
| CS270103B1 true CS270103B1 (en) | 1990-06-13 |
Family
ID=5380992
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CS863944A CS270103B1 (en) | 1986-05-29 | 1986-05-29 | wall / l-> 6 / -beta-o-gluco- / 1 - ► 3 / -beta-o-glucan yeast |
Country Status (1)
| Country | Link |
|---|---|
| CS (1) | CS270103B1 (en) |
-
1986
- 1986-05-29 CS CS863944A patent/CS270103B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CS394486A1 (en) | 1989-11-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zhang et al. | Carboxymethylated β-glucans from mushroom sclerotium of Pleurotus tuber-regium as novel water-soluble anti-tumor agent | |
| Zeković et al. | Natural and modified (1→ 3)-β-D-glucans in health promotion and disease alleviation | |
| AU642023B2 (en) | Anti-virus agent | |
| Sandula et al. | Mitogenic activity of particulate yeast β-(1→ 3)-D-glucan and its water-soluble derivatives | |
| Liepins et al. | Drying enhances immunoactivity of spent brewer's yeast cell wall β-d-glucans | |
| DK0759089T3 (en) | Treatment of Glucans with Enzymes | |
| CN108727516A (en) | A kind of chitosan chlorogenic acid salt and its preparation method and application | |
| GB2048918A (en) | Physiologically active polysaccharide | |
| CN1583802A (en) | Method for extracting beta-1,3-dextran | |
| CS270103B1 (en) | wall / l-> 6 / -beta-o-gluco- / 1 - ► 3 / -beta-o-glucan yeast | |
| CN106519060A (en) | Preparation of carboxymethyl curdlan | |
| CN101891837A (en) | A kind of carboxymethylated bifidobacterium exopolysaccharide and its preparation method and application | |
| US20240373876A1 (en) | High immune yeast cell wall, and preparation method therefor and use thereof | |
| CN109762078A (en) | A kind of hydroxypropyltrimethylammonium chloride chitosan-carboxylated polysaccharide complex salt and preparation method and application | |
| Suzuki et al. | Effect of the interferon inducer, dextran phosphate, on influenza virus infection in mice | |
| CN1177044C (en) | Radish chitin bindin and its preparing process | |
| CN107987179B (en) | Application of low-sulfated fucan in preparation of immunopotentiator | |
| US4973581A (en) | Glucan derivatives having tumoricidal activity | |
| CN101472602A (en) | Use of fungal polysaccharides as pharmaceutical composition or food complements | |
| CN101851259A (en) | Selenium chitooligosaccharide and its preparation method | |
| KR100543692B1 (en) | Solubilization method of insoluble beta-glucan isolated from basidiomycete | |
| CN1133653C (en) | Ganoderma lucidum α-(1→3)-D-glucan sulfate derivative and its use and preparation method | |
| CN113943381A (en) | Purification method, molecular structure and use of a novel sea urchin gonadal polysaccharide | |
| US5185327A (en) | Glucan derivatives having tumoricidal activity | |
| CN1313500C (en) | Preparation of chitin oligose compound |