CS272151B1 - Method of making catgut - Google Patents

Abstract

The solution concerns a method of producing catgut. The hitherto technologically unusable submucosal layers of bovine intestines or serous layers of sheep intestines are processed in the form of strips, separately or in a mixture with technologically used strips of the serous layer of bovine intestines or the submucosal layer of sheep intestines. Their strength in the wet and dry state is increased to technological usability by creating cross-links of collagen, its cross-linking and shrinkage by the action of solutions of organic substances aldehydes or dialdehydes or ketoaldehydes or oxyaldehydes or acetals and inorganic substances nitrates or chlorides or sulfates. In addition to the main features mentioned, the solution contains other variants. The solution can be used in healthcare and the consumer industry.

Description

The invention relates to a process for the production of catgut.

The hitherto known processes for the production of catgut of normal absorption and of astonished catgut with delayed absorption are based on the serous layer of bovine intestines and the submucosal layer of sheep intestines as technologically applicable. During production, the submucosal layer of bovine intestines and the serous layer of sheep intestines are eliminated due to their low wet strength and, in the case of the submucosal layer of bovine intestines, also due to its fluctuating thickness, and have therefore not been used for production.

These drawbacks are substantially eliminated according to the invention of a method for producing catgut, the essence of which is that the hitherto technologically unusable layers of bovine or intestinal bovine intestinal layers are treated in strips, alone or in a mixture with technologically commonly used bovine intestinal or submucosal layers. layers of mutton. Their wet and dry strength is increased to technological applicability by creating collagen crosslinks, crosslinking and shrinking. This is achieved by the action of solutions of organic substances aldehydes or dialdehydes or ketoaldehydes or oxyaldehydes or acetals and inorganic substances nitrates or chlorides or sulphates. Aqueous solutions contain organic substances in a concentration of 0.05 to 3%. Examples are acetaldehyde, glutaraldehyde, glyoxal, methylglyoxal, diacetyl, formaldehyde-dimethyl acetal, glycoldehyde. Aqueous solutions contain inorganic substances in an amount of 0.05 to 5%. Examples are potassium nitrate, aluminum sulphate, sodium sulphate, magnesium sulphate, manganese sulphate, ammonium sulphate and magnesium chloride. The inorganic solutions are either separate or added to the organic solutions.

The method for the production of catgut according to the invention makes it possible to process a submucosal layer of bovine intestines or a serous layer of sheep intestines, from which it has not yet been produced. These layers have not yet been used and have been disposed of as biological waste in remediation facilities. Their processing in mixtures with commonly used layers increases their wet strength and reduces calibration losses due to the original fluctuating thickness of the submucosal layer of bovine intestines. By the action of the solutions of organic substances according to the invention, the formation of cross-links of collagen and its crosslinking is achieved, which is favorably manifested by the increased strength in the wet and dry state. The addition of the inorganic salt solutions according to the invention increases the effects of the previous organic substances and causes a partial shrinkage of the cells, which is manifested by a further increase in the wet strength. By increasing the wet strength, it is possible to process these layers at all, since otherwise they would usually crack if they were shortened into fibers even when stretched on dryers. By processing the serous layers of bovine intestines themselves and the submucosal layer of sheep intestines, which are the subject of conventional processing, an improvement in the quality of the catgut and a reduction in production losses are achieved using the catgut production method according to the invention. Example 1

The lamellae, i.e. strips of the submucosal layer of bovine intestines and lamellae of the serosal layer of bovine intestines, processed according to the author's certificate No. 248 353, method of treatment of animal binder and equipment for performing this method, . Anhydrous 1% sodium sulfate and 1% sodium chloride solutions were impregnated on them. After 1 hour of material movement the solution was drained and replaced with 1% acetaldehyde solution, after 1 hour of movement in the solution the solution was drained and replaced with 2% potassium nitrate solution, after 2 hours of movement the solution was drained and replaced with deionized water, after 1 hour of movement and replaced with fresh deionized water, after 16 hours of material movement, the material was removed. The lamellae were twisted into fibers, which were hung and dried on frames according to the author's certificate No. 243 602, frame for drying catgut and other fibers or longitudinal shapes, further processing was according to the usual production procedure.

CS 272151 Bl 2

Example 2

The treated lamellae of only the submucosal layer of the bovine intestines were attached to the loops in pairs and hung in bath vessels. They were soaked in 0.5% methyl glyoxal solution, after 1 hour of material movement the solution was drained and replaced with 0.5% sodium tetraborate solution, after 3 hours of material movement the solution was drained and replaced with 2% magnesium chloride, after 1 hour The material was drained and replaced with deionized water, after 1 hour of material movement it was drained and replaced with fresh deionized water, after 16 hours of material movement the material was removed. 0 _D racování lamellae · twisting and drying, and other procedures were as in the Example 1.

Example 3 * ’'

The treated lamellas of only the serous layers of mutton intestines were attached to the loops in pairs and hung in bath vessels. They were impregnated with 1.5% glycolldehyde solution with 0.75% ammonium sulfate solution, after 2 hours of material movement the solution was drained and replaced with 1.5% sodium tetraborate solution, after 1 hour of material movement the solution was drained and replaced with deionized water , after 3 hours of material movement it was drained and replaced with fresh deionized water, after 8 hours of material movement the material was removed. The treatment of the slats and their further processing was as in Example 1.

Example '4' ''.

• The treated lamellae of the submucosal layer of bovine intestines and the serous layer of mutton intestines were tied together to the loops in a ratio. 1 ·: 2 and hung in bath containers. A solution of 0.5% glutaraldehyde and 0.05% potassium carbonate was impregnated on them. After 3 hours of material movement, the solution was drained and replaced with 0.5% aluminum sulfate solution. After 1 hour of material movement, the solution was drained and replaced with 0.05% formaldehyde dimethyl acetal solution. After 4 hours of material movement, the solution was drained and replaced with deionized water, after 3 hours of material movement it was drained and replaced with fresh deionized water, after 5 hours movement of the material, the material was removed. The processing of the slats and their further processing was as in Example 1. ’

Example 5. _. ,. . .

The treated lamellae of the serous layer of sheep intestines and the serous layer of bovine intestines were tied together to loops in a ratio of 1: 1 and hung in bath vessels. A 0.2% glyoxal solution was soaked in them. After 1 hour of material movement, the solution was drained and replaced with 0.7% magnesium sulfate solution. After 5 hours of material movement, the solution was drained and replaced with deionized water, after 2 hours of material movement it was drained and replaced with fresh deionized water, after 8 hours of material movement it was removed. The treatment of the slats and their further processing was as in Example 1.

Example 6

The treated lamellae of the serosal layer of mutton intestines and the submucosal layer of mutton intestines were connected together to loops in a ratio of 3: 1 and hung in bath vessels. A 0.05% diacetyl solution was soaked in them. After 6 hours of material movement, the solution was drained and replaced with 1% manganese sulfate solution. After 1 hour of material movement, the solution was drained and replaced with deionized water, after 4 hours of material movement it was drained and replaced with fresh deionized water, after 4 hours of material movement it was removed. The processing of the lamellas and their further processing was as in Example 1. In the evaluation of all examples, it was found that there were no higher losses in twisting and hanging on the drying frames than in the processing of the existing technologically used layers.

The calibration losses were 8% in Example 1, 20% in Example 2, 10% in Example 3, 18% in Example 4, 6% in Example 5, and 7% in Example 6. Catgut, made only from the normal serosal layer of bovine intestines, always shows calibration losses, but to a lesser extent. In the case of final grinding with a centerless system, the calibration losses would be further reduced in all the above examples. The fibers from all the examples, in the final processing according to the regulation, met in sterile form the standards for catgut in the tests of physical1 »

CS 272151 Bl nich., Microbiological and biological,

In the production of catgut from the submucosal layer of bovine intestines and the serous layer of mutton intestines, a very gentle processing procedure of these layers is necessary, especially by replacing mechanical deburring by a chemical process. the lamellae of these layers consist of two or more layers and are attached to loops. They can be bound separately, which increases the calibration losses in the submucosal layer of bovine intestines, or in a mixture with lamellae of the serosal layer of bovine intestines or with lamellae of sheep intestines, which reduces these losses. The duration of action of the solutions and washing is governed by the type and amount of material to be treated, the temperature and concentration of the solutions, as well as the equipment of the baths.

The above examples and procedures illustrate but do not limit the invention.

The invention can be used for the production of eatgut, music and sports strings,

Claims (3)

OBJECT OF THE INVENTION 1, Method for producing catgut, characterized in that hitherto technologically unusable layers of the mucosal bovine intestinal or serous layer of sheep intestines are processed in the form of strips, alone or in a mixture with technologically used strips of bovine intestinal layer or serous mucosal layer in aqueous solutions containing organic or inorganic substances, 2. Process according to claim 1, characterized in that the aqueous solutions containing organic substances are concentrations of 0.05 to 3% by weight, for example acetaldehyde, glutaraldehyde, glyoxal, methylglyoxal, diacetyl, formaldehyde dimethyl acetal, glycoldehyde. 3.. Process according to items 1 and 2, characterized in that the aqueous solutions contain inorganic substances in an amount of 0.05 to 5% by weight, for example potassium nitrate, aluminum sulphate, sodium, magnesium, manganese, ammonium, magnesium chloride and are either separate, or added to solutions of organic substances.