CS80492A3 - Process for preparing glutarylacylase in large quantities - Google Patents

Process for preparing glutarylacylase in large quantities

Info

Publication number
CS80492A3
CS80492A3 CS92804A CS80492A CS80492A3 CS 80492 A3 CS80492 A3 CS 80492A3 CS 92804 A CS92804 A CS 92804A CS 80492 A CS80492 A CS 80492A CS 80492 A3 CS80492 A3 CS 80492A3
Authority
CS
Czechoslovakia
Prior art keywords
glutarylacylase
preparing
large quantities
dsm
coli
Prior art date
Application number
CS92804A
Other languages
Czech (cs)
Inventor
Klaus-Peter Dr Koller
Gunther Johannes Dr Riess
Werner Dr Aretz
Original Assignee
Hoechst Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst Ag filed Critical Hoechst Ag
Publication of CS80492A3 publication Critical patent/CS80492A3/en
Publication of CZ284405B6 publication Critical patent/CZ284405B6/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

The gene which encodes glutaryl acylase (GA) and is contained in the plasmid pCM145 (DSM 6409) permits the expression of GA in E. coli in high yields. <IMAGE>
CS92804A 1991-03-18 1992-03-17 PLASMID pCM145, GENE STRUCTURE CONTAINING GA-GENE AND PROCESS FOR PREPARING GLUTARYLACYLASE CZ284405B6 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4108823 1991-03-18
DE4136389A DE4136389A1 (en) 1991-03-18 1991-11-05 PROCESS FOR THE PRODUCTION OF GLUTARYLACYLASE IN LARGE QUANTITIES

Publications (2)

Publication Number Publication Date
CS80492A3 true CS80492A3 (en) 1992-10-14
CZ284405B6 CZ284405B6 (en) 1998-11-11

Family

ID=25902010

Country Status (15)

Country Link
EP (1) EP0504798B1 (en)
JP (1) JP3366664B2 (en)
KR (1) KR100211002B1 (en)
CN (1) CN1065292A (en)
AT (1) ATE145238T1 (en)
CA (1) CA2063176C (en)
CY (1) CY2115B1 (en)
CZ (1) CZ284405B6 (en)
DE (2) DE4136389A1 (en)
DK (1) DK0504798T3 (en)
ES (1) ES2096671T3 (en)
GR (1) GR3021610T3 (en)
HK (1) HK1006853A1 (en)
IL (1) IL101258A0 (en)
SK (1) SK280242B6 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4337787A1 (en) * 1993-11-05 1995-05-11 Hoechst Ag Oxygen-dependent fermentation of microorganisms
IT1269180B (en) * 1994-01-14 1997-03-21 Mini Ricerca Scient Tecnolog PROCESS FOR THE PRODUCTION OF THE 7BETA ACID ENZYME - (4-CARBOXYBUTANAMIDE) -PHALOSPORANIC ACYLASE
DE4437420A1 (en) * 1994-10-19 1996-04-25 Hoechst Ag Improved, induction-free process for the genetic engineering of glutarylamidase
US5766871A (en) * 1996-11-27 1998-06-16 Food Industry Research And Development Institute Screening and characterization of glutaryl-7-aminocephalosporanic acid acylase
DE19915938A1 (en) 1999-04-09 2000-10-19 Aventis Pharma Gmbh Production of pancreatic procarboxypeptidase B, isoforms and muteins thereof and their use
DE19924632B4 (en) * 1999-05-28 2006-04-13 Sanofi-Aventis Deutschland Gmbh A method of delaying the deactivation of glutarylamidase during enzyme catalysis

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HU194307B (en) * 1983-09-16 1988-01-28 Richter Gedeon Vegyeszet Process for producing plasmidvectors of high copy number
DE3926076A1 (en) * 1989-04-21 1990-10-25 Boehringer Mannheim Gmbh RECOMBINANT DNA AND EXPRESSION VECTOR

Also Published As

Publication number Publication date
CN1065292A (en) 1992-10-14
DE4136389C2 (en) 1993-07-01
CA2063176C (en) 2004-06-01
CA2063176A1 (en) 1992-09-19
IL101258A0 (en) 1992-11-15
DK0504798T3 (en) 1997-04-07
KR100211002B1 (en) 1999-07-15
EP0504798A1 (en) 1992-09-23
SK280242B6 (en) 1999-10-08
ES2096671T3 (en) 1997-03-16
GR3021610T3 (en) 1997-02-28
EP0504798B1 (en) 1996-11-13
JPH05207879A (en) 1993-08-20
CZ284405B6 (en) 1998-11-11
KR920018222A (en) 1992-10-21
HK1006853A1 (en) 1999-03-19
DE4136389A1 (en) 1992-10-08
ATE145238T1 (en) 1996-11-15
CY2115B1 (en) 2002-04-26
JP3366664B2 (en) 2003-01-14
DE59207509D1 (en) 1996-12-19

Similar Documents

Publication Publication Date Title
MY102555A (en) An improved derivative of the tryptophan operon for expression of fused gene products
EP0722342A4 (en) REGULATION OF bcl-2 GENE EXPRESSION
ZA918462B (en) Expression system.
EP0562508A3 (en) Protein with bone formation ability and process for its production
IL101258A0 (en) Process for the preparation of glutarylacylase in large quantities
EP0226979A3 (en) Process for producing the protein of an aequorin activity by using a photoprotein aequorin gene
DE3666838D1 (en) Plasmid vectors for expression in escherichia coli and/or bacillus subtilis
FI102544B1 (en) Process for producing an E. coli strain
IL82147A0 (en) Substituted oxime-ethers,their preparation and their use for controlling plant growth
EP0545511A3 (en) Process for the preparation of mono-n-substituted tetraazamacrocycles
SI0677584T1 (en) Stable mutants of D-N-alpha-carbamylase
AU575331B2 (en) Improved e. coli plasmid containing modified 5&#39; cipoprotein sequence
GB2275472A (en) DNA fragment comprising a gene encoding ethylene forming enzyme of bacteria and the use therof
GR3002696T3 (en) Process for production of isulin-like growth factor i and plasmid for production thereof
EP0499993A3 (en) Method for producing human nerve growth factor-2
EP0064681A3 (en) Novel recombinant dna
EP0292806A3 (en) Substituted vinyl cephalosporins, process for their preparation and their use as medicines
EP0341674A3 (en) Cloned tryptophan synthase gene and recombinant plasmid containing the same
AU5652286A (en) Media for increased yield of protein free of N-terminal methionine from recombinant escherichia coli
AU3058489A (en) Methods for controlling norleucine content in polypeptides
HU9203485D0 (en) Method for producing new plasmides
EP0614908A3 (en) Method of preparation of 3-beta-aminocholanic acid derivatives.
GB8823728D0 (en) Recombinant plasmid dna ppr-il2-19, coding for synthesis of human interleukin-2, method of bacteria escherichia coli vniigenetika vl 903 (ppr-il2-19) as producer of human interleukin-2, containing it
AU6636486A (en) Top-bottom blow process with carbon dioxide greater than 98 percent from bottom
GB8823729D0 (en) Recombinant plasmid dna ppr-il2-19, coding for synthesis of human interleukin-2, method of bacteria escherichia coli vniigenetika vl 903 (ppr-il2-19) as producer of human interleukin-2, containing it

Legal Events

Date Code Title Description
IF00 In force as of 2000-06-30 in czech republic
MM4A Patent lapsed due to non-payment of fee

Effective date: 20080317