CU20100263A7 - Producción y almacenamiento mejorados de proteínas en plantas - Google Patents

Producción y almacenamiento mejorados de proteínas en plantas

Info

Publication number
CU20100263A7
CU20100263A7 CU20100263A CU20100263A CU20100263A7 CU 20100263 A7 CU20100263 A7 CU 20100263A7 CU 20100263 A CU20100263 A CU 20100263A CU 20100263 A CU20100263 A CU 20100263A CU 20100263 A7 CU20100263 A7 CU 20100263A7
Authority
CU
Cuba
Prior art keywords
storage
proteins
plants
seed
improved production
Prior art date
Application number
CU20100263A
Other languages
English (en)
Inventor
Eliot M Herman
Monica A Schmidt
Original Assignee
Us Gov Agriculture
Donald Danforth Plant Sci Ct
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Gov Agriculture, Donald Danforth Plant Sci Ct filed Critical Us Gov Agriculture
Publication of CU20100263A7 publication Critical patent/CU20100263A7/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Nutrition Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Se describe una planta dicotiledónea transgénica que tiene una deficiencia de una o más proteínas de almacenamiento de semilla vegetal, que tiene además una construcción de polinucleótido transgénica que comprende un marco de lectura abierto enlazado operablemente a un promotor de proteína de almacenamiento y una secuencia de senal ER. La construcción de polinucleótido codifica para un producto de proteína que puede acumularse a altos niveles en la semilla. Se proporcionan también métodos para producir una proteína heteróloga en una semilla vegetal.
CU20100263A 2008-06-28 2010-12-28 Producción y almacenamiento mejorados de proteínas en plantas CU20100263A7 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US7661608P 2008-06-28 2008-06-28

Publications (1)

Publication Number Publication Date
CU20100263A7 true CU20100263A7 (es) 2012-06-21

Family

ID=41444990

Family Applications (1)

Application Number Title Priority Date Filing Date
CU20100263A CU20100263A7 (es) 2008-06-28 2010-12-28 Producción y almacenamiento mejorados de proteínas en plantas

Country Status (20)

Country Link
US (1) US20100313307A1 (es)
EP (1) EP2307547A4 (es)
JP (1) JP2011526155A (es)
KR (1) KR20110044211A (es)
CN (1) CN102137932A (es)
AP (1) AP2011005557A0 (es)
AR (1) AR072391A1 (es)
AU (1) AU2009261943A1 (es)
BR (1) BRPI0914824A2 (es)
CA (1) CA2729375A1 (es)
CL (1) CL2010001598A1 (es)
CO (1) CO6341485A2 (es)
CU (1) CU20100263A7 (es)
DO (1) DOP2010000399A (es)
EA (1) EA201170104A1 (es)
EC (1) ECSP11010793A (es)
IL (1) IL210210A0 (es)
MX (1) MX2010014541A (es)
PE (1) PE20110562A1 (es)
WO (2) WO2009158694A1 (es)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8741591B2 (en) 2009-10-09 2014-06-03 The Research Foundation For The State University Of New York pH-insensitive glucose indicator protein
MX2013002586A (es) * 2010-10-01 2013-03-21 Novozymes Inc Variantes de beta-glucosidasa y polinucleotidos que codifican las mismas.
JP5809810B2 (ja) * 2011-02-22 2015-11-11 本田技研工業株式会社 タンパク質の植物細胞内への蓄積方法
WO2013149801A1 (en) * 2012-04-05 2013-10-10 Basf Plant Science Company Gmbh Fungal resistant plants expressing hydrophobin
EP2914719A1 (en) * 2012-10-31 2015-09-09 Danisco US Inc. Compositions and methods of use
KR101449155B1 (ko) * 2012-12-06 2014-10-13 주식회사 바이오앱 식물체에서 목적 단백질의 번역 효율을 증진시키기 위한 염기서열
CN106480089A (zh) * 2016-12-30 2017-03-08 上海交通大学 一种提高大豆含硫氨基酸含量及降低过敏原蛋白的方法
CN109486851B (zh) * 2018-10-12 2022-04-01 武汉禾元生物科技股份有限公司 一种提高胚乳生物反应器中重组蛋白表达水平的方法
CN109220805A (zh) * 2018-11-05 2019-01-18 贵州大学 一种红豆树组培苗瓶外生根方法
IL265841A (en) * 2019-04-03 2020-10-28 Yeda Res & Dev Plant expressing animal milk proteins
KR102213745B1 (ko) 2019-04-16 2021-02-09 주식회사 바이오앱 돼지 유행성 설사병 바이러스 백신 조성물 및 이의 제조 방법
WO2020256372A1 (ko) 2019-06-17 2020-12-24 주식회사 바이오앱 아프리카 돼지열병의 진단을 위한 항원 생산용 재조합 벡터 및 이의 용도
CN110122333A (zh) * 2019-06-17 2019-08-16 西安同人五凤农业有限公司 一种凤凰木种子促进发芽生根的方法
US11326176B2 (en) * 2019-11-22 2022-05-10 Mozza Foods, Inc. Recombinant micelle and method of in vivo assembly
US12359212B2 (en) 2019-11-22 2025-07-15 Mozza Foods, Inc. Recombinant micelle and method of in vivo assembly
JP2023548301A (ja) * 2020-10-28 2023-11-16 パイオニア ハイ－ブレッド インターナショナル， インコーポレイテッド ダイズにおけるレグヘモグリン
WO2022093977A1 (en) * 2020-10-30 2022-05-05 Fortiphyte, Inc. Pathogen resistance in plants
KR102630105B1 (ko) * 2020-11-26 2024-01-29 전남대학교산학협력단 Cel12A 단백질을 포함하는 셀룰로오스 분해용 조성물 및 이의 제조방법
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
EP4393505A1 (en) 2021-08-27 2024-07-03 Bioapplications Inc. Vaccine for preventing african swine fever, comprising african swine fever virus-derived antigen protein
AU2022375642A1 (en) * 2021-10-25 2024-05-16 The Regents Of The University Of California Vectors and methods for improved dicot plant transformation frequency
KR102954443B1 (ko) * 2023-07-18 2026-04-20 메디프로젠 주식회사 저장단백질 함량이 저감된 콩 형질전환체 및 이의 용도
KR102726138B1 (ko) 2023-10-17 2024-11-14 대한민국 백시니아 바이러스 재조합 a33r 단백질 및 이를 포함하는 오르소폭스바이러스 항체 진단 키트

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2092069A1 (en) * 1992-03-27 1993-09-28 Asako Iida An expression plasmid for seeds
US6326527B1 (en) * 1993-08-25 2001-12-04 Dekalb Genetics Corporation Method for altering the nutritional content of plant seed
US5850016A (en) * 1996-03-20 1998-12-15 Pioneer Hi-Bred International, Inc. Alteration of amino acid compositions in seeds
JP3928811B2 (ja) * 1996-06-14 2007-06-13 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 特定のクラスのダイズ種子タンパク質遺伝子の抑制
JP3451284B2 (ja) * 2000-06-15 2003-09-29 名古屋大学長 ファイトスルフォカイン前駆体遺伝子のプロモーター
US7615624B2 (en) * 2000-06-23 2009-11-10 Syngenta Participations Ag Arabidopsis derived promoters for regulation of plant expression
US6855871B2 (en) * 2000-08-21 2005-02-15 Pioneer Hi-Bred International, Inc. Methods of increasing polypeptide accumulation in plants
JP4565231B2 (ja) * 2000-08-22 2010-10-20 独立行政法人農業生物資源研究所 外来遺伝子産物を植物の種子中に高度に蓄積させる方法
AU2002244071A1 (en) * 2001-02-14 2002-08-28 Ventria Bioscience Expression system for seed proteins
EP1402038A2 (en) * 2001-06-22 2004-03-31 Syngenta Participations AG Identification and characterization of plant genes
ATE455176T1 (de) * 2002-09-03 2010-01-15 Sungene Gmbh Transgene expressionskassetten zur expression von nukleinsäuren in nicht-reproduktiven blütengeweben von pflanzen
CA2410702A1 (en) * 2002-11-26 2004-05-26 Illimar Altosaar Production of human granulocyte macrophage-colony stimulating factor (gm-csf) in the seeds of transgenic rice plants
JP2007503212A (ja) * 2003-08-27 2007-02-22 オーアールエフ・リフタエクニ・エイチエフ 内在性貯蔵タンパク質の標的化抑制を介した、植物種子中で異種ポリペプチドの蓄積を亢進させる方法
CN1950510B (zh) * 2004-03-08 2011-10-05 先正达合作有限公司 富含谷氨酰胺的玉米种子蛋白质和启动子
US20090130710A1 (en) * 2005-05-09 2009-05-21 Allison Kermode Enhancing vegetative protein production in transgenic plants using seed specific promoters

Also Published As

Publication number Publication date
CL2010001598A1 (es) 2011-07-15
EP2307547A4 (en) 2011-06-22
PE20110562A1 (es) 2011-08-11
CN102137932A (zh) 2011-07-27
DOP2010000399A (es) 2012-11-15
AU2009261943A1 (en) 2009-12-30
MX2010014541A (es) 2011-07-29
EP2307547A1 (en) 2011-04-13
KR20110044211A (ko) 2011-04-28
AP2011005557A0 (en) 2011-02-28
CO6341485A2 (es) 2011-11-21
EA201170104A1 (ru) 2011-08-30
JP2011526155A (ja) 2011-10-06
WO2009158694A1 (en) 2009-12-30
BRPI0914824A2 (pt) 2015-12-01
ECSP11010793A (es) 2011-07-29
WO2009158716A1 (en) 2009-12-30
IL210210A0 (en) 2011-03-31
US20100313307A1 (en) 2010-12-09
CA2729375A1 (en) 2009-12-30
AR072391A1 (es) 2010-08-25

Similar Documents

Publication Publication Date Title
DOP2010000399A (es) Produccion y almacenamiento mejorados de proteinas en plantas
AR115692A2 (es) Método para la producción de promotores constitutivos de plantas, constructos y células transgénicas
WO2011011412A3 (en) Transgenic plants having increased biomass
WO2013086499A3 (en) Transgenic plants having altered biomass composition
CL2007000525A1 (es) Método para aumentar el rendimiento de semillas de plantas que consiste en aumentar la expresión de una secuencia de ácido nucleico que codifica un dominio del factor de transcripción del polipéptido myb; método de producción de planta transgénica; y uso de la secuencia de ácido nucleico para aumentar el rendimiento de semillas de plantas.
AR060435A1 (es) Polipeptidos relacionados con esteres y metodos para su uso en plantas
NZ599844A (en) Processing biomass
UY32220A (es) Material vegetal, plantas y metodo para producir la planta que contiene propiedades alteradas de lignina
MX2010003870A (es) Metodos para controlar el tama?o de la semilla y el organo de la planta.
AR078433A1 (es) Metodo de preparacion de proteinas derivadas de plantas
MX2012003402A (es) Plantas que tienen mejores rasgos relacionados con el rendimiento y un metodo para producirlas.
AR095746A1 (es) Modificación de plantas para producir farneseno y otros terpenoides
CL2016000902A1 (es) Molécula de ácido nucleico aislada que codifica una enzima delta-9-desaturasa, método para generar plantas que comprenden dicho ácido nucleico, y procedimiento para disminuir la cantidad de ácidos grasos saturados en una planta. (divisional de sol. n°3665-12).
BR112012008021A2 (pt) plantas transgênicas que possuem composição de biomassa aperfeiçoada
AR069202A1 (es) Composiciones y metodos para alterar la produccion de pigmento en plantas
MX2011007597A (es) Manipulacion genetica de los factores de transcripcion de nf-yb para el aumento de la resistencia a la sequia y aumento de rendimiento en las plantas transgenicas.
AR071624A1 (es) Plantas que tienen rasgos mejorados relacionados con el rendimiento y un metodo para obtenerlas mediante la expresion de un acido nucleico que codifica dna proteasa especifica de ubiquitina (ubp) de origen vegetal (ej. oryza)
AR077312A1 (es) Plantas con contenido modificado de lignina y metodos para su produccion
CL2023003886A1 (es) Plantas leñosas con crecimiento mejorado
CL2009002148A1 (es) Polinucleotido aislado capaz de modular la biomasa, la produccion de semilla y la tolerancia a la sequia, frio, congelamiento y/o salinidad en una planta, polipeptido codificado, constructo genetico y de expresion, celula hospedadora, celula de planta y planta que lo comprenden, metodos para producir y esleccionar dichas plantas.
AR066786A1 (es) Composiciones para la produccion de soja con contenido de aceite elevado
AR087220A1 (es) Plantas de maiz que tienen un genoma parcial o completamente multiplicado y sus usos
WO2010029357A3 (en) Method for the alteration of plants using cle polypeptides / peptides
AR075240A1 (es) Modificacion de enzima multidominio para la expresion en plantas
AR088744A1 (es) Plantas que tienen mejores rasgos relacionados con el rendimiento y un metodo para producirlas