CU22276A1 - Procedure for the expression of heterologous genes in pichia pastoris yeast, expression vectors and transformed micro-organisms. - Google Patents

Procedure for the expression of heterologous genes in pichia pastoris yeast, expression vectors and transformed micro-organisms.

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Publication number
CU22276A1
CU22276A1 CU1990007A CU1990007A CU22276A1 CU 22276 A1 CU22276 A1 CU 22276A1 CU 1990007 A CU1990007 A CU 1990007A CU 1990007 A CU1990007 A CU 1990007A CU 22276 A1 CU22276 A1 CU 22276A1
Authority
CU
Cuba
Prior art keywords
gene
expression
pichia pastoris
procedure
expression vectors
Prior art date
Application number
CU1990007A
Other languages
Spanish (es)
Inventor
Gonzalez Vladimir Wong
Clark Emilio Margolles
Boada Julio Marcos Delgado
Madrazo Isis Del Carmen Torrens
Lopez Tania Gonzalez
Rodriguez Alejandro Silva
Menendez Alina Seralena
Martinez Maria Elena Gonzalez
Martinez Luis Saturnino Herrera
Original Assignee
Cigb
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cigb filed Critical Cigb
Priority to CU1990007A priority Critical patent/CU22276A1/en
Priority to DK91200074T priority patent/DK0438200T3/en
Priority to JP3014953A priority patent/JPH05184352A/en
Priority to AT91200074T priority patent/ATE220721T1/en
Priority to EP91200074A priority patent/EP0438200B1/en
Priority to ES91200074T priority patent/ES2180528T3/en
Priority to DE69133066T priority patent/DE69133066T2/en
Publication of CU22276A1 publication Critical patent/CU22276A1/en

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

ESTA RELACIONADA CON LA RAMA DE LA BIOTECNOLOGIA Y ESPECIFICAMENTE CON LA COMO FACTOR DE CRECIMIENTO EPIDERMICO HUMANO (EGFH) Y SUCROSA INVERTASA. TECNOLOGIA DEL ADN RECOMBINANTE PARA LA PRODUCCION DE PROTEINAS HETEROLOGAS. EL OBJETIVO TECNICO DE LA MISMA CONSISTE EN DESARROLLAR UN PROCEDIMIENTO ALTAMENTE EFICIENTE PARA LA EXPRESION DE GENES HETEROLOGOS, EMPLEANDOSE COMO HOSPEDERO UN MUTANTE AUXOTROFICO HIS3 DE LA CEPA BKM-90 DE PICHIA PASTORIS Y COMO ADN RECOMBINANTE PARA SU TRANSFORMACION UN VECTOR INTEGRATIVO QUE CONTIENE UN MARCADOR DE SELECCION FUNCIONAL DE SACCHAROMYCES CEREVISIAE, EL PROMOTOR DEL GEN ALCOHOL OXIDASA (AOX 1) DE LA PROPIA LEVADURA, COMO TERMINADOR DE LA TRANSCRIPCION EL DE LA GLICERALDEHIDO-3-FOSFATO DESHIDROGENASA (GAP) DE S.CEREVISIAE, O EL QUE PORTE EL GEN HETEROLOGO A EXPRESAR, Y OTRA SECUENCIA HOMOLOGA AL GENOMA DE PICHIA PASTORIS QUE SIRVE PARA LA INTEGRACION DEL MISMO. LOS VECTORES DE EXPRESION EMPLEADOS CONTIENEN ADEMAS UN GEN HETEROLOGO ACOPLADO AL PEPTIDO SEÑAL DEL GEN SUCROSA INVERTASA (SUC2) DE S. CEREVISIAE. ESTE PROC EDIMIENTO GARANTIZA QUE LOS PRODUCTOS POLIPEPTIDICOS SEAN RECUPERABLES BAJO CONDICIONES REGULABLES Y CON ALTOS RENDIMIENTOS. LAS CEPAS OBTENIDAS, OBJETO DE LA PRESENTE INVENCI PUEDEN SER EMPLEADAS PARA LA PRODUCCION EN GRAN ESCALA DE PRODUCTOS FARMACEUTICOS O DE ENZIMAS DE INTERES INDUSTRIAL\!IT IS RELATED TO THE BRANCH OF BIOTECHNOLOGY AND SPECIFICALLY TO IT AS A HUMAN EPIDERMIC GROWTH FACTOR (EGFH) AND SUCROSA INVERTASA. RECOMBINANT DNA TECHNOLOGY FOR THE PRODUCTION OF HETEROLOGICAL PROTEINS. THE TECHNICAL OBJECTIVE OF THE SAME IS TO DEVELOP A HIGHLY EFFICIENT PROCEDURE FOR THE EXPRESSION OF HETEROLOGICAL GENES, USING AS A HOS3 AUXOTHROPHIC MUTANT OF THE PICHIA PASTORIS A BKM-90 MULTIPLE SOURCE AND A RECOMBINANT DNA RECOMBINANT FOR A OF FUNCTIONAL SELECTION OF SACCHAROMYCES CEREVISIAE, THE PROMOTER OF THE GENE ALCOHOL OXIDASE (AOX 1) OF ITS OWN YEAST, AS THE TRANSCRIPTION TERMINATOR THAT OF THE GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (GAP) OF S. ELEREVISIA HETEROLOGIST TO EXPRESS, AND ANOTHER HOMOLOGICAL SEQUENCE TO THE GENE OF PICHIA PASTORIS THAT SERVES FOR THE INTEGRATION OF THE SAME. THE EMPLOYED EXPRESSION VECTORS FURTHER CONTAIN A HETEROLOGIC GENE COUPLED TO THE PEPTIDE SIGNAL OF THE SUCROSA INVERTASE (SUC2) GENE OF S. CEREVISIAE. THIS PROCEDURE GUARANTEES THAT THE POLYPEPTIDIC PRODUCTS ARE RECOVERABLE UNDER ADJUSTABLE CONDITIONS AND WITH HIGH PERFORMANCE. THE STRAINS OBTAINED, OBJECT OF THE PRESENT INVENTION, MAY BE USED FOR THE LARGE-SCALE PRODUCTION OF PHARMACEUTICAL PRODUCTS OR ENZYMES OF INDUSTRIAL INTEREST!

CU1990007A 1990-01-16 1990-01-16 Procedure for the expression of heterologous genes in pichia pastoris yeast, expression vectors and transformed micro-organisms. CU22276A1 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CU1990007A CU22276A1 (en) 1990-01-16 1990-01-16 Procedure for the expression of heterologous genes in pichia pastoris yeast, expression vectors and transformed micro-organisms.
DK91200074T DK0438200T3 (en) 1990-01-16 1991-01-16 Method for expression of heterologous genes in the yeast Pichia pastoris, expression vectors and transformed microorganisms
JP3014953A JPH05184352A (en) 1990-01-16 1991-01-16 Method for expressing dissimilar gene in pichia pastoris yeast, expression vector and transforming microorganism
AT91200074T ATE220721T1 (en) 1990-01-16 1991-01-16 METHOD FOR EXPRESSING HETEROLOGICAL GENES IN THE YEAST PICHIA PASTORIS, EXPRESSION VECTORS AND TRANSFORMED MICROORGANISMS
EP91200074A EP0438200B1 (en) 1990-01-16 1991-01-16 Method for the expression of heterologous genes in the yeast Pichia pastoris, expression vectors and transformed microorganisms
ES91200074T ES2180528T3 (en) 1990-01-16 1991-01-16 METHOD OF EXPRESSION OF HETEROLOGICAL GENES IN PICHIA PASTORIS YEAST, EXPRESSION VECTORS AND TRANSFORMED MICROORGANISMS.
DE69133066T DE69133066T2 (en) 1990-01-16 1991-01-16 Process for the expression of heterological genes in the yeast Pichia Pastoris, expression process and transformed microorganisms

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU1990007A CU22276A1 (en) 1990-01-16 1990-01-16 Procedure for the expression of heterologous genes in pichia pastoris yeast, expression vectors and transformed micro-organisms.

Publications (1)

Publication Number Publication Date
CU22276A1 true CU22276A1 (en) 1995-01-31

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CU1990007A CU22276A1 (en) 1990-01-16 1990-01-16 Procedure for the expression of heterologous genes in pichia pastoris yeast, expression vectors and transformed micro-organisms.

Country Status (1)

Country Link
CU (1) CU22276A1 (en)

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