CU22642A1 - Secuencia de adnc derivadas del genoma del virus de la hepatitis c y su uso - Google Patents
Secuencia de adnc derivadas del genoma del virus de la hepatitis c y su usoInfo
- Publication number
- CU22642A1 CU22642A1 CU1996119A CU1996119A CU22642A1 CU 22642 A1 CU22642 A1 CU 22642A1 CU 1996119 A CU1996119 A CU 1996119A CU 1996119 A CU1996119 A CU 1996119A CU 22642 A1 CU22642 A1 CU 22642A1
- Authority
- CU
- Cuba
- Prior art keywords
- hepatitis
- virus
- particle
- development
- hcv
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 3
- 241000711549 Hepacivirus C Species 0.000 title abstract 2
- 239000002299 complementary DNA Substances 0.000 abstract 2
- 239000012634 fragment Substances 0.000 abstract 2
- 239000002245 particle Substances 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 abstract 1
- 229930006000 Sucrose Natural products 0.000 abstract 1
- 108020000999 Viral RNA Proteins 0.000 abstract 1
- 238000004458 analytical method Methods 0.000 abstract 1
- 239000000427 antigen Substances 0.000 abstract 1
- 230000000890 antigenic effect Effects 0.000 abstract 1
- 102000036639 antigens Human genes 0.000 abstract 1
- 108091007433 antigens Proteins 0.000 abstract 1
- 239000003443 antiviral agent Substances 0.000 abstract 1
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 238000001493 electron microscopy Methods 0.000 abstract 1
- 208000006454 hepatitis Diseases 0.000 abstract 1
- 231100000283 hepatitis Toxicity 0.000 abstract 1
- 229920001184 polypeptide Polymers 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 229940021993 prophylactic vaccine Drugs 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
- 239000005720 sucrose Substances 0.000 abstract 1
- 230000001225 therapeutic effect Effects 0.000 abstract 1
- 229940021747 therapeutic vaccine Drugs 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/24011—Flaviviridae
- C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
- C12N2770/24222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Gastroenterology & Hepatology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
La presente invención provee una serie de secuencias de ADNc derivadas del virus de la hepatitis C, denominado VHC-Cu, y la expresión de los antígenos codificados por dichas secuencias. El ARN viral del VHC-Cu se extrajo del suero de un paciente con hepatitis no-A, no-B. El ADNc se amplificó por PCR y los fragmentos obtenidos fueron clonados y secuenciados. Los fragmentos que codificaban para polipéptidos antigénicos fueron expresados en levadura, obteniéndose una proteína multimérica o una partícula semejante a virus. El análisis por microscopía electrónica mostró una partícula de 20 a 60 nm de diámetro, con densidad de flotación entre 1.16 y 1.30 g/cm3 en gradientes de sacarosa y cloruro de cesio. Esta invención puede ser utilizada en la medicina para el desarrollo de vacunas terapéuticas y profilácticas, para la producción de proteínas con interés diagnóstico, para el desarrollo de anticuerpos policlonales, anticuerpos monoclonales naturales o recombinantes, y agentes antivirales.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CU1996119A CU22642A1 (es) | 1996-12-12 | 1996-12-12 | Secuencia de adnc derivadas del genoma del virus de la hepatitis c y su uso |
| PCT/CU1997/000007 WO1998025960A1 (es) | 1996-12-12 | 1997-12-12 | Secuencias derivadas del genoma del virus de la hepatitis c y su uso |
| AU53974/98A AU5397498A (en) | 1996-12-12 | 1997-12-12 | Sequences derived from the genome of the c hepatitis virus, and use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CU1996119A CU22642A1 (es) | 1996-12-12 | 1996-12-12 | Secuencia de adnc derivadas del genoma del virus de la hepatitis c y su uso |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CU22642A1 true CU22642A1 (es) | 2000-12-22 |
Family
ID=46093345
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CU1996119A CU22642A1 (es) | 1996-12-12 | 1996-12-12 | Secuencia de adnc derivadas del genoma del virus de la hepatitis c y su uso |
Country Status (3)
| Country | Link |
|---|---|
| AU (1) | AU5397498A (es) |
| CU (1) | CU22642A1 (es) |
| WO (1) | WO1998025960A1 (es) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CU23244A1 (es) * | 2001-07-16 | 2007-10-17 | Ct Ingenieria Genetica Biotech | Formulacion vacunal potenciada por la combinacion de un adn con un antigeno |
| CA2566506A1 (en) * | 2004-06-01 | 2005-12-15 | Innogenetics N.V. | Peptides for inducing a ctl and/or htl response to hepatitis c virus |
| CU23470A1 (es) | 2004-08-11 | 2009-12-17 | Ct Ingenieria Genetica Biotech | Vectores vivos recombinantes y su uso en composiciones farmacéuticas contra el virus de la hepatitis c |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR8807310A (pt) * | 1987-11-18 | 1990-03-13 | Chiron Corp | Polinucleotidio,polinucleotidio recombinante,vetor recombinante,celula hospedeira,sistema de expressao recombinante,celula transformada,polipeptidio,hcv,preparacao de polipeptidio,polipeptidio de hcv,polipeptidio purificado,polipeptidio recombinante,polipeptidio de fusao,anticorpo monoclonal,preparacao purificada de anticorpos policlonais,particula,sonda de polinucleotidio,kit anticorpo para epetopo de hcv,metodo para producao de polipeptidio,metodo para deteccao de acidos nucleicos de hcv,imunoensaio para detectar antigeno de hcv,imunoensaio para detectar anticorpos dirigidos contra antigeno de hcv,vacina para tratamento de infeccao de hcv,celula,celula infectada com hcv,metodo para producao de anticorpos para hcv e metodo para isolar cdna derivado do genoma de um agente infeccioso nao identificado |
| JP3268502B2 (ja) * | 1990-06-12 | 2002-03-25 | 徹雄 中村 | 非ａ非ｂ型肝炎ウイルス関連ポリヌクレオチド、ポリペプ タイド |
| EP0461863A1 (en) * | 1990-06-12 | 1991-12-18 | Immuno Japan Inc. | Oligonucleotide primers, and their application for high-fidelity detection of non-A, non-B hepatitis virus |
| CU22290A1 (es) * | 1990-10-08 | 1995-01-31 | Cigb | Procedimiento para la obtencion de antigeno de superficie del virus de la hepatitis b de superior capacidad inmunoganica y su uso en un preparado vacunal |
| DE69223562T2 (de) * | 1991-08-27 | 1998-06-04 | Hoffmann La Roche | Verfahren und Reagenzien zum Nachweis von Hepatitis C |
| EP0532258A2 (en) * | 1991-09-09 | 1993-03-17 | Immuno Japan Inc. | Oligonucleotides and determination system of HCV genotypes |
| JPH06153961A (ja) * | 1992-11-27 | 1994-06-03 | Imuno Japan:Kk | Ｃ型肝炎ウイルス部分遺伝子、ならびにｃ型肝炎ウイルス遺 伝子の高感度検出法 |
| JP3472319B2 (ja) * | 1993-06-18 | 2003-12-02 | 株式会社先端生命科学研究所 | 非ａ非ｂ型肝炎ウイルス抗原のアミノ酸配列を含む組換えポリペプチド及び該ポリペプチドを用いた非ａ非ｂ型肝炎ウイルス抗体の検出方法 |
| JPH07322881A (ja) * | 1994-05-31 | 1995-12-12 | S R L:Kk | オリゴヌクレオチド、それから成るｃ型肝炎診断試薬及びそれを用いたｃ型肝炎の診断方法 |
-
1996
- 1996-12-12 CU CU1996119A patent/CU22642A1/es unknown
-
1997
- 1997-12-12 AU AU53974/98A patent/AU5397498A/en not_active Abandoned
- 1997-12-12 WO PCT/CU1997/000007 patent/WO1998025960A1/es not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO1998025960A1 (es) | 1998-06-18 |
| AU5397498A (en) | 1998-07-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kratz et al. | Native display of complete foreign protein domains on the surface of hepatitis B virus capsids | |
| SE9004010D0 (sv) | Viralt medel | |
| DK1033995T3 (da) | Hele eller sprængte insektceller som adjuvans for antigener | |
| FI935808A0 (fi) | Hepatit-C-virus (HCV) polypeptider | |
| BR9813416A (pt) | Composições e métodos de nodavìrus recombinante | |
| DE69111801D1 (de) | Vakzin gegen hepatitis b. | |
| CN107921115A (zh) | 新颖杆状病毒载体及使用方法 | |
| RU2319505C2 (ru) | Очищенные оболочечные белки вируса гепатита с для диагностического и терапевтического применения | |
| Pumpens et al. | Evaluation of HBs, HBc, and frCP virus-like particles for expression of human papillomavirus 16 E7 oncoprotein epitopes | |
| Pishraft-Sabet et al. | Enhancement of HCV polytope DNA vaccine efficacy by fusion to an N-terminal fragment of heat shock protein gp96 | |
| DK461884A (da) | Beskyttende peptidantigen | |
| Patton et al. | Nucleotide and amino acid sequence analysis of the rotavirus nonstructural RNA-binding protein NS35 | |
| KR910019641A (ko) | 사람 유두종 바이러스(hpv) 18의 단백질상의 혈청 반응성 에피토프 | |
| KR880701283A (ko) | Aids의 원인이 되는 비루스의 당단백질을 암호화하는 비루스 벡터, 백신 및 항체 | |
| Feitelson et al. | The nature of polypeptides larger in size than the major surface antigen components of hepatitis b and like viruses in ground squirrels, woodchucks, and ducks | |
| CA2098253C (en) | Method for producing ectoprotein of hepatitis c virus | |
| ES2581576T3 (es) | Polinucleótidos que permiten la expresión y la secreción de pseudovirus recombinantes que contienen epítopos extraños, su producción y utilización | |
| ATE290399T1 (de) | Hbv/hcv virus-ohnliche particle | |
| Kos et al. | Induction of primary anti‐viral cytotoxic T cells by in vitro stimulation with short synthetic peptide and interleukin‐7 | |
| DK35891A (da) | Praktisk taget rent, antigenisk peptid eller protein med en epitop af haemophilus influenzae, dets fremstilling og anvendelse | |
| CU22290A1 (es) | Procedimiento para la obtencion de antigeno de superficie del virus de la hepatitis b de superior capacidad inmunoganica y su uso en un preparado vacunal | |
| DE68926274D1 (de) | Rekombinante HBsAg Hybridpartikel, die morphologische Eigenschaften von HBsAg-Antigen haben und eine immunogene Sequenz enthalten, die neutralisierende Antikörper gegen HIV induziert oder die von diesen Antikörpern erkannt ist, Nukleotide Sequenzen, die diese Partikel codieren und sie enthaltende Impfstoffe | |
| AR009855A1 (es) | SECUENCIAS NUCLEOTIDICAS DE ADNc DERIVADOS DEL GENOMA DE LA HEPATITIS C, POLINUCLEOTIDOS RECOMBINANTES, MICROORGANISMOS TRANSFORMADOS, SUSTANCIASPROTEINACEAS, MUTLIMEROS PROTEICOS, CRISTALES PROTEICOS Y UNA MOLECULA RECOMBINANTE | |
| ZA202310905B (en) | Adenoviral vectors encoding hepatitis b viral antigens fused to herpes virus glycoprotein d and methods of using the same | |
| Basak et al. | Construction and characterization of encapsidated poliovirus replicons that express biologically active murine interleukin-2 |