CU23149A3 - PROCESS FOR PURIFICATION OF PHARMACOLOGICALLY ACTIVE PROTEINS THROUGH CATION EXCHANGE CHROMATOGRAPHY - Google Patents

PROCESS FOR PURIFICATION OF PHARMACOLOGICALLY ACTIVE PROTEINS THROUGH CATION EXCHANGE CHROMATOGRAPHY

Info

Publication number
CU23149A3
CU23149A3 CU112A CU20020112A CU23149A3 CU 23149 A3 CU23149 A3 CU 23149A3 CU 112 A CU112 A CU 112A CU 20020112 A CU20020112 A CU 20020112A CU 23149 A3 CU23149 A3 CU 23149A3
Authority
CU
Cuba
Prior art keywords
purification
pharmacologically active
proteins
cation exchange
exchange chromatography
Prior art date
Application number
CU112A
Other languages
Spanish (es)
Inventor
Giuseppe Claudio Viscomi
Lucia Scapol
Original Assignee
Alfa Waddermann S P A
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alfa Waddermann S P A filed Critical Alfa Waddermann S P A
Publication of CU23149A3 publication Critical patent/CU23149A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/811—Interferon

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

El objeto de la invencion es un proceso para la purificacion de proteínas farmacologicamente activas basado en el uso de cromatografía de intercambio cationico llevada a cabo en una matriz solida a un pH mAs bAsico, ej. superior, con respecto al pH correspondiente al punto isoelÉctrico pI, de las proteínas al ser purificadas, pH en el cual sin embargo dichas proteínas quedan todavía absorbidas. Las soluciones buffer con los valores de pH y de fuerza ionica ajustada de tiempo en tiempo al tipo de proteína farmacologicamente activa a ser purificadas son usadas para obtener tal resultado. El proceso estA principalmente dirigido a la purificacion del interferon y proteínas de albUmina.The object of the invention is a process for the purification of pharmacologically active proteins based on the use of cation exchange chromatography carried out in a solid matrix at a more basic pH, eg. higher, with respect to the pH corresponding to the isoelectric point pI, of the proteins to be purified, pH at which, however, said proteins are still absorbed. Buffer solutions with the pH and ionic strength values adjusted from time to time to the type of pharmacologically active protein to be purified are used to obtain such a result. The process is primarily aimed at the purification of interferon and albumin proteins.

CU112A 2001-07-06 2002-06-06 PROCESS FOR PURIFICATION OF PHARMACOLOGICALLY ACTIVE PROTEINS THROUGH CATION EXCHANGE CHROMATOGRAPHY CU23149A3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT2001BO000426A ITBO20010426A1 (en) 2001-07-06 2001-07-06 PHARMACOLOGICALLY ACTIVE PROTEIN PURIFICATION PROCESS BY CATIONIC EXCHANGE CHROMATOGRAPHY

Publications (1)

Publication Number Publication Date
CU23149A3 true CU23149A3 (en) 2006-06-29

Family

ID=11439471

Family Applications (1)

Application Number Title Priority Date Filing Date
CU112A CU23149A3 (en) 2001-07-06 2002-06-06 PROCESS FOR PURIFICATION OF PHARMACOLOGICALLY ACTIVE PROTEINS THROUGH CATION EXCHANGE CHROMATOGRAPHY

Country Status (27)

Country Link
US (1) US6866782B2 (en)
EP (1) EP1273592B1 (en)
JP (1) JP3940037B2 (en)
KR (1) KR100771252B1 (en)
CN (1) CN100484954C (en)
AR (1) AR034663A1 (en)
AT (1) ATE307824T1 (en)
BG (1) BG65748B1 (en)
BR (1) BR0202431A (en)
CA (1) CA2388716C (en)
CU (1) CU23149A3 (en)
DE (1) DE60206847T2 (en)
DK (1) DK1273592T3 (en)
DZ (1) DZ3236A1 (en)
EG (1) EG23150A (en)
ES (1) ES2250544T3 (en)
HR (1) HRP20020480B1 (en)
HU (1) HUP0202124A2 (en)
IT (1) ITBO20010426A1 (en)
ME (1) MEP3408A (en)
MX (1) MXPA02006303A (en)
PL (1) PL209954B1 (en)
RS (1) RS51512B (en)
RU (1) RU2274471C2 (en)
SI (1) SI1273592T1 (en)
SK (1) SK287827B6 (en)
UY (1) UY27354A1 (en)

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WO2004103519A2 (en) * 2003-05-16 2004-12-02 Cryobiophysica, Inc. External gradient chromatofocusing
US20080269469A1 (en) * 2004-03-30 2008-10-30 Hiroshi Yanagisawa Method of Separating Protein
US7449116B2 (en) * 2004-10-01 2008-11-11 Agilent Technologies, Inc. Methods and systems for protein separation
US7943046B2 (en) * 2004-10-01 2011-05-17 Agilent Technologies, Inc Methods and systems for on-column protein delipidation
WO2008051178A2 (en) * 2006-04-12 2008-05-02 Savient Pharmaceuticals, Inc. Purification of proteins with cationic surfactant
WO2009073975A1 (en) * 2007-12-10 2009-06-18 National Research Council Of Canada Production of recombinant interferon proteins
DE102009032179A1 (en) 2009-07-07 2011-01-13 Biogenerix Ag Process for purifying interferon beta
CN101768601B (en) * 2010-02-01 2013-08-07 山东泉港药业有限公司 Method for producing recombinant human serum albumin-interferon alpha 2b
WO2013004587A1 (en) * 2011-07-01 2013-01-10 F. Hoffmann-La Roche Ag Method for separation of monomeric polypeptides from aggregated polypeptides
US8921113B2 (en) 2012-12-21 2014-12-30 Dionex Corporation Buffer kit and method of generating a linear pH gradient
US9650412B2 (en) * 2013-03-08 2017-05-16 Genzyme Corporation Integrated continuous manufacturing of therapeutic protein drug substances
CN104236983A (en) * 2013-06-14 2014-12-24 中国科学院大连化学物理研究所 Method for removing ionic liquid containing long alkyl chain in solution sample
TWI671312B (en) 2014-01-17 2019-09-11 美商健臻公司 Sterile chromatography and manufacturing processes
TWI709569B (en) 2014-01-17 2020-11-11 美商健臻公司 Sterile chromatography resin and use thereof in manufacturing processes
WO2016079598A1 (en) * 2014-11-18 2016-05-26 Unichem Laboratories Limited An improved process for the preparation of pharmacopoeial grade interferon alpha 2b
CN106496302B (en) * 2015-09-08 2021-12-10 三生国健药业（上海）股份有限公司 Method for purifying protein by ion exchange chromatography
GB201517450D0 (en) * 2015-10-02 2015-11-18 Ipsen Biopharm Ltd Method
KR20180085737A (en) * 2015-12-09 2018-07-27 바스프 에스이 Method for Purifying Proteins from Fermented Solids under Desorption Conditions
GB2568936B (en) 2017-12-01 2019-12-25 Ford Global Tech Llc Liquid cooled injector
AU2019332764B2 (en) 2018-08-31 2025-05-29 Genzyme Corporation Sterile chromatography resin and use thereof in manufacturing processes
US11022585B2 (en) * 2019-06-09 2021-06-01 Dionex Corporation Methods and systems for optimizing buffer conditions with liquid chromatography
CN111138523A (en) * 2019-12-10 2020-05-12 天津生机集团股份有限公司 Method for purifying and preparing recombinant chicken interferon α from recombinant chicken interferon α renaturation solution
CN113968900B (en) * 2020-07-23 2023-07-25 中元汇吉生物技术股份有限公司 Method for purifying C1q protein
CN115918902A (en) * 2022-12-19 2023-04-07 新疆农业科学院农业质量标准与检测技术研究所 Method for pretreating grape dehydration and obtaining high-phenol grape extract through microwave-assisted wave diffusion gravity method

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DE1617401A1 (en) * 1966-05-02 1972-02-17 Centre Nat Rech Scient Process for the production of interferon preparations
US4228154A (en) * 1979-02-26 1980-10-14 Armour Pharmaceutical Company Purification of plasma albumin by ion exchange chromatography
US4534906A (en) * 1982-11-01 1985-08-13 Genentech, Inc. Removal of impurities from human leukocyte interferon preparations
DE3515336C2 (en) * 1985-04-27 1994-01-20 Boehringer Ingelheim Int Process for the preparation and purification of â-interferon
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US5037644A (en) * 1986-10-27 1991-08-06 Cetus Corporation Pharmaceutical compositions of recombinant interleukin-2 and formulation processes
US4732683A (en) * 1986-12-02 1988-03-22 Biospectrum, Inc. Purification method for alpha interferon
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US5521287A (en) * 1992-05-20 1996-05-28 The Green Cross Corporation Recombinant human serum albumin, process for producing the same and pharmaceutical preparation containing the same
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JP3702474B2 (en) * 1994-06-01 2005-10-05 三菱ウェルファーマ株式会社 Method for producing serum albumin preparation
RU2081122C1 (en) * 1994-06-16 1997-06-10 Институт биоорганической химии им.М.М.Шемякина и Ю.А.Овчинникова РАН Method of isolation and purification of insulin or its biotechnological precursors
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Also Published As

Publication number Publication date
BG106768A (en) 2003-05-30
EP1273592B1 (en) 2005-10-26
RU2002117383A (en) 2004-01-27
JP2003096092A (en) 2003-04-03
CN100484954C (en) 2009-05-06
HRP20020480B1 (en) 2006-11-30
HU0202124D0 (en) 2002-09-28
EP1273592A2 (en) 2003-01-08
UY27354A1 (en) 2003-04-30
US6866782B2 (en) 2005-03-15
KR100771252B1 (en) 2007-10-30
CA2388716C (en) 2008-01-08
HUP0202124A2 (en) 2003-10-28
ITBO20010426A0 (en) 2001-07-06
DZ3236A1 (en) 2005-04-20
ES2250544T3 (en) 2006-04-16
ATE307824T1 (en) 2005-11-15
HRP20020480A2 (en) 2003-02-28
MEP3408A (en) 2010-10-10
JP3940037B2 (en) 2007-07-04
KR20030005005A (en) 2003-01-15
AR034663A1 (en) 2004-03-03
BR0202431A (en) 2003-09-09
EP1273592A3 (en) 2003-03-19
DE60206847D1 (en) 2005-12-01
MXPA02006303A (en) 2003-02-10
RS51512B (en) 2011-06-30
BG65748B1 (en) 2009-09-30
RU2274471C2 (en) 2006-04-20
CA2388716A1 (en) 2003-01-06
SI1273592T1 (en) 2006-02-28
YU50502A (en) 2005-09-19
PL209954B1 (en) 2011-11-30
PL354769A1 (en) 2003-01-13
CN1396176A (en) 2003-02-12
ITBO20010426A1 (en) 2003-01-06
SK8592002A3 (en) 2003-01-09
DE60206847T2 (en) 2006-07-06
DK1273592T3 (en) 2006-01-02
EG23150A (en) 2004-05-31
US20030010715A1 (en) 2003-01-16
SK287827B6 (en) 2011-11-04

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