CY1111111T1 - Διαδικασια για εκχυλιση πρωτεϊνων - Google Patents

Διαδικασια για εκχυλιση πρωτεϊνων

Info

Publication number
CY1111111T1
CY1111111T1 CY20111100054T CY111100054T CY1111111T1 CY 1111111 T1 CY1111111 T1 CY 1111111T1 CY 20111100054 T CY20111100054 T CY 20111100054T CY 111100054 T CY111100054 T CY 111100054T CY 1111111 T1 CY1111111 T1 CY 1111111T1
Authority
CY
Cyprus
Prior art keywords
protein
proteins
extraction
procedure
acidic solution
Prior art date
Application number
CY20111100054T
Other languages
English (en)
Inventor
Richard L Gehant
Original Assignee
Genentech, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech, Inc. filed Critical Genentech, Inc.
Publication of CY1111111T1 publication Critical patent/CY1111111T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against enzymes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Η εφεύρεση περιλαμβάνει διαδικασία για εκχύλιση πρωτεΐνης-στόχου από κύτταρα Ε. coli η οποία περιλαμβάνει μείωση του pΗ διαλύματος ολόκληρων κυττάρων Ε. coli για να σχηματιστεί όξινο διάλυμα, διάσπαση των κυττάρων για να εκλυθεί η πρωτεΐνη στο όξινο διάλυμα, και διαχωρισμό των κυτταρικών κατάλοιπων από την εκλυμένη πρωτεΐνη για να αποκτηθεί πρωτεϊνικό προϊόν εμπλουτισμένο με την ετερόλογη πρωτεΐνη-στόχο. Η εφεύρεση περιλαμβάνει επίσης προσθήκη ενισχυτή διαλυτότητας.
CY20111100054T 2002-09-06 2011-01-17 Διαδικασια για εκχυλιση πρωτεϊνων CY1111111T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US40865302P 2002-09-06 2002-09-06
EP03752068A EP1539798B1 (en) 2002-09-06 2003-09-05 Process for protein extraction

Publications (1)

Publication Number Publication Date
CY1111111T1 true CY1111111T1 (el) 2015-06-11

Family

ID=31978651

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20111100054T CY1111111T1 (el) 2002-09-06 2011-01-17 Διαδικασια για εκχυλιση πρωτεϊνων

Country Status (14)

Country Link
US (6) US6967241B2 (el)
EP (2) EP2292637B1 (el)
JP (2) JP5519089B2 (el)
AT (1) ATE489400T1 (el)
AU (2) AU2003270380B2 (el)
CA (1) CA2497209C (el)
CY (1) CY1111111T1 (el)
DE (1) DE60335119D1 (el)
DK (2) DK2292637T3 (el)
ES (2) ES2355088T3 (el)
HU (1) HUE027196T2 (el)
PT (1) PT1539798E (el)
SI (2) SI2292637T1 (el)
WO (1) WO2004022581A1 (el)

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CN110878118B (zh) * 2018-09-06 2023-04-18 深圳翰宇药业股份有限公司 度拉糖肽的纯化方法与纯化试剂
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TW202333581A (zh) * 2021-12-24 2023-09-01 南韓商Ｃｊ第一製糖股份有限公司 由發酵液製備含胺基酸製品之方法

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Also Published As

Publication number Publication date
EP1539798A4 (en) 2006-02-08
CA2497209C (en) 2015-04-28
US20130172535A1 (en) 2013-07-04
US7713707B2 (en) 2010-05-11
ES2355088T3 (es) 2011-03-22
ES2565781T3 (es) 2016-04-06
JP2010111700A (ja) 2010-05-20
AU2003270380A1 (en) 2004-03-29
AU2003270380B2 (en) 2010-07-01
WO2004022581A1 (en) 2004-03-18
HK1076287A1 (en) 2006-01-13
DK2292637T3 (en) 2016-04-04
JP5519089B2 (ja) 2014-06-11
EP2292637A3 (en) 2012-04-11
US20040049012A1 (en) 2004-03-11
SI1539798T1 (sl) 2011-03-31
PT1539798E (pt) 2011-02-23
US20060106205A1 (en) 2006-05-18
SI2292637T1 (sl) 2016-04-29
DK1539798T3 (da) 2011-02-28
ATE489400T1 (de) 2010-12-15
EP1539798A1 (en) 2005-06-15
JP2006513144A (ja) 2006-04-20
US8383773B2 (en) 2013-02-26
CA2497209A1 (en) 2004-03-18
EP2292637B1 (en) 2016-01-06
US20100222558A1 (en) 2010-09-02
US6967241B2 (en) 2005-11-22
US20110124845A1 (en) 2011-05-26
HK1151041A1 (en) 2012-01-20
DE60335119D1 (de) 2011-01-05
EP2292637A2 (en) 2011-03-09
US8940875B2 (en) 2015-01-27
AU2010202423A1 (en) 2010-07-01
US7662934B2 (en) 2010-02-16
EP1539798B1 (en) 2010-11-24
US8232374B2 (en) 2012-07-31
HUE027196T2 (hu) 2016-10-28
US20070031930A1 (en) 2007-02-08

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