DD145326A5 - Verfahren zur bestimmung von glutamat-oxalacetat-transaminase glutamat-pyruvat-transaminase - Google Patents

Verfahren zur bestimmung von glutamat-oxalacetat-transaminase glutamat-pyruvat-transaminase Download PDF

Info

Publication number: DD145326A5
Authority: DD; German Democratic Republic
Prior art keywords: glutamate; transaminase; mmol; tetrazolium; pyruvate
Prior art date: 1978-08-08

Application number

DD79214827A

Other languages

German (de)

English (en)

Inventor

Ulfert Deneke

Peter Stahl

Walter Schneider

Original Assignee

Boehringer Mannheim Gmbh

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1978-08-08

Filing date

1979-08-06

Publication date

1980-12-03

1979-08-06 Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh

1980-12-03 Publication of DD145326A5 publication Critical patent/DD145326A5/de

Links

238000000034 method Methods 0.000 title claims description 35
101710098398 Probable alanine aminotransferase, mitochondrial Proteins 0.000 title claims description 34
102000003929 Transaminases Human genes 0.000 title claims description 16
108090000340 Transaminases Proteins 0.000 title claims description 16
UPLLQJUZZIYKHI-DFWYDOINSA-N (2s)-2-aminopentanedioic acid;2-oxobutanedioic acid Chemical compound OC(=O)CC(=O)C(O)=O.OC(=O)[C@@H](N)CCC(O)=O UPLLQJUZZIYKHI-DFWYDOINSA-N 0.000 title description 2
238000012360 testing method Methods 0.000 claims description 26
229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 claims description 25
229930195712 glutamate Natural products 0.000 claims description 21
WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims description 20
XJLXINKUBYWONI-DQQFMEOOSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-DQQFMEOOSA-N 0.000 claims description 18
239000003153 chemical reaction reagent Substances 0.000 claims description 16
LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 claims description 15
229940067621 aminobutyrate Drugs 0.000 claims description 15
239000000872 buffer Substances 0.000 claims description 15
RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 claims description 15
102000005566 Succinate-Semialdehyde Dehydrogenase Human genes 0.000 claims description 13
108010084086 Succinate-Semialdehyde Dehydrogenase Proteins 0.000 claims description 13
238000006243 chemical reaction Methods 0.000 claims description 13
KHPXUQMNIQBQEV-UHFFFAOYSA-N oxaloacetic acid Chemical compound OC(=O)CC(=O)C(O)=O KHPXUQMNIQBQEV-UHFFFAOYSA-N 0.000 claims description 13
125000003831 tetrazolyl group Chemical group 0.000 claims description 9
239000012992 electron transfer agent Substances 0.000 claims description 8
VMGAPWLDMVPYIA-HIDZBRGKSA-N n'-amino-n-iminomethanimidamide Chemical compound N\N=C\N=N VMGAPWLDMVPYIA-HIDZBRGKSA-N 0.000 claims description 7
BOLDJAUMGUJJKM-LSDHHAIUSA-N renifolin D Natural products CC(=C)[C@@H]1Cc2c(O)c(O)ccc2[C@H]1CC(=O)c3ccc(O)cc3O BOLDJAUMGUJJKM-LSDHHAIUSA-N 0.000 claims description 7
239000004094 surface-active agent Substances 0.000 claims description 7
FSVCQIDHPKZJSO-UHFFFAOYSA-L nitro blue tetrazolium dichloride Chemical compound [Cl-].[Cl-].COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC=CC=2)C=2C=CC(=CC=2)[N+]([O-])=O)=CC=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=C([N+]([O-])=O)C=C1 FSVCQIDHPKZJSO-UHFFFAOYSA-L 0.000 claims description 5
QLAJNZSPVITUCQ-UHFFFAOYSA-N 1,3,2-dioxathietane 2,2-dioxide Chemical compound O=S1(=O)OCO1 QLAJNZSPVITUCQ-UHFFFAOYSA-N 0.000 claims description 4
239000007983 Tris buffer Substances 0.000 claims description 4
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 4
DGEZNRSVGBDHLK-UHFFFAOYSA-N [1,10]phenanthroline Chemical compound C1=CN=C2C3=NC=CC=C3C=CC2=C1 DGEZNRSVGBDHLK-UHFFFAOYSA-N 0.000 claims description 3
BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 claims description 3
UIUJIQZEACWQSV-UHFFFAOYSA-N succinic semialdehyde Chemical compound OC(=O)CCC=O UIUJIQZEACWQSV-UHFFFAOYSA-N 0.000 claims description 3
-1 2,5-diphenyl-tetrazolium trifluoride Chemical compound 0.000 claims description 2
AZKSAVLVSZKNRD-UHFFFAOYSA-M 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide Chemical compound [Br-].S1C(C)=C(C)N=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=CC=C1 AZKSAVLVSZKNRD-UHFFFAOYSA-M 0.000 claims description 2
125000006306 4-iodophenyl group Chemical group [H]C1=C([H])C(*)=C([H])C([H])=C1I 0.000 claims description 2
239000008366 buffered solution Substances 0.000 claims description 2
VASKZFMOJFLZDZ-UHFFFAOYSA-N 5-phenyl-1h-tetrazol-1-ium;bromide Chemical compound [Br-].[NH2+]1N=NN=C1C1=CC=CC=C1 VASKZFMOJFLZDZ-UHFFFAOYSA-N 0.000 claims 1
PTFCDOFLOPIGGS-UHFFFAOYSA-N Zinc dication Chemical compound [Zn+2] PTFCDOFLOPIGGS-UHFFFAOYSA-N 0.000 claims 1
230000000694 effects Effects 0.000 description 20
238000005259 measurement Methods 0.000 description 18
BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 14
229940076788 pyruvate Drugs 0.000 description 12
238000010521 absorption reaction Methods 0.000 description 11
KHPXUQMNIQBQEV-UHFFFAOYSA-L oxaloacetate(2-) Chemical compound [O-]C(=O)CC(=O)C([O-])=O KHPXUQMNIQBQEV-UHFFFAOYSA-L 0.000 description 11
230000015572 biosynthetic process Effects 0.000 description 10
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210000002966 serum Anatomy 0.000 description 10
239000000975 dye Substances 0.000 description 9
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239000000523 sample Substances 0.000 description 9
102000004190 Enzymes Human genes 0.000 description 7
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150000007857 hydrazones Chemical class 0.000 description 7
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 6
BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 description 6
238000011156 evaluation Methods 0.000 description 6
229950006238 nadide Drugs 0.000 description 6
239000000243 solution Substances 0.000 description 6
RXGJTUSBYWCRBK-UHFFFAOYSA-M 5-methylphenazinium methyl sulfate Chemical compound COS([O-])(=O)=O.C1=CC=C2[N+](C)=C(C=CC=C3)C3=NC2=C1 RXGJTUSBYWCRBK-UHFFFAOYSA-M 0.000 description 4
239000012954 diazonium Substances 0.000 description 4
150000001989 diazonium salts Chemical class 0.000 description 4
JORABGDXCIBAFL-UHFFFAOYSA-M iodonitrotetrazolium chloride Chemical compound [Cl-].C1=CC([N+](=O)[O-])=CC=C1N1[N+](C=2C=CC(I)=CC=2)=NC(C=2C=CC=CC=2)=N1 JORABGDXCIBAFL-UHFFFAOYSA-M 0.000 description 4
DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 description 4
229920004890 Triton X-100 Polymers 0.000 description 3
230000007812 deficiency Effects 0.000 description 3
238000001514 detection method Methods 0.000 description 3
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
101000950981 Bacillus subtilis (strain 168) Catabolic NAD-specific glutamate dehydrogenase RocG Proteins 0.000 description 2
102000016901 Glutamate dehydrogenase Human genes 0.000 description 2
DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 2
229910019142 PO4 Inorganic materials 0.000 description 2
239000002202 Polyethylene glycol Substances 0.000 description 2
241000269319 Squalius cephalus Species 0.000 description 2
238000002835 absorbance Methods 0.000 description 2
229940009098 aspartate Drugs 0.000 description 2
239000000987 azo dye Substances 0.000 description 2
230000008033 biological extinction Effects 0.000 description 2
230000008878 coupling Effects 0.000 description 2
238000010168 coupling process Methods 0.000 description 2
238000005859 coupling reaction Methods 0.000 description 2
238000007654 immersion Methods 0.000 description 2
239000000463 material Substances 0.000 description 2
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
239000010452 phosphate Substances 0.000 description 2
229920001223 polyethylene glycol Polymers 0.000 description 2
229940054269 sodium pyruvate Drugs 0.000 description 2
239000000758 substrate Substances 0.000 description 2
JNYAEWCLZODPBN-JGWLITMVSA-N (2r,3r,4s)-2-[(1r)-1,2-dihydroxyethyl]oxolane-3,4-diol Chemical class OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O JNYAEWCLZODPBN-JGWLITMVSA-N 0.000 description 1
FCERNNLMTCOKHX-UHFFFAOYSA-O 1,5-diphenyl-2H-tetrazol-1-ium Chemical compound C1=CC=CC=C1C1=[NH+]N=NN1C1=CC=CC=C1 FCERNNLMTCOKHX-UHFFFAOYSA-O 0.000 description 1
CMCBDXRRFKYBDG-UHFFFAOYSA-N 1-dodecoxydodecane Chemical compound CCCCCCCCCCCCOCCCCCCCCCCCC CMCBDXRRFKYBDG-UHFFFAOYSA-N 0.000 description 1
KPGXRSRHYNQIFN-UHFFFAOYSA-L 2-oxoglutarate(2-) Chemical compound [O-]C(=O)CCC(=O)C([O-])=O KPGXRSRHYNQIFN-UHFFFAOYSA-L 0.000 description 1
SHVOAEFVBWFZKE-UHFFFAOYSA-N 5-phenyl-2H-tetrazol-1-ium chloride Chemical compound [Cl-].C1=CC=CC=C1C1=[NH+]NN=N1 SHVOAEFVBWFZKE-UHFFFAOYSA-N 0.000 description 1
WDJHALXBUFZDSR-UHFFFAOYSA-N Acetoacetic acid Natural products CC(=O)CC(O)=O WDJHALXBUFZDSR-UHFFFAOYSA-N 0.000 description 1
QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
GAWIXWVDTYZWAW-UHFFFAOYSA-N C[CH]O Chemical group C[CH]O GAWIXWVDTYZWAW-UHFFFAOYSA-N 0.000 description 1
101710088194 Dehydrogenase Proteins 0.000 description 1
108091006149 Electron carriers Proteins 0.000 description 1
WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
239000004471 Glycine Substances 0.000 description 1
QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
102000003855 L-lactate dehydrogenase Human genes 0.000 description 1
108700023483 L-lactate dehydrogenases Proteins 0.000 description 1
JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
102000013460 Malate Dehydrogenase Human genes 0.000 description 1
108010026217 Malate Dehydrogenase Proteins 0.000 description 1
208000029578 Muscle disease Diseases 0.000 description 1
BWJJSYVJOLHQFF-AMLDTQNSSA-N N'-(4-iodoanilino)-N-(4-nitrophenyl)iminobenzenecarboximidamide Chemical compound C1=CC=C(C=C1)/C(=N/NC2=CC=C(C=C2)I)/N=NC3=CC=C(C=C3)[N+](=O)[O-] BWJJSYVJOLHQFF-AMLDTQNSSA-N 0.000 description 1
WVVOBOZHTQJXPB-UHFFFAOYSA-N N-anilino-N-nitronitramide Chemical compound [N+](=O)([O-])N(NC1=CC=CC=C1)[N+](=O)[O-] WVVOBOZHTQJXPB-UHFFFAOYSA-N 0.000 description 1
GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
239000002253 acid Substances 0.000 description 1
150000007513 acids Chemical class 0.000 description 1
235000004279 alanine Nutrition 0.000 description 1
229910052783 alkali metal Inorganic materials 0.000 description 1
150000001340 alkali metals Chemical class 0.000 description 1
150000001346 alkyl aryl ethers Chemical class 0.000 description 1
238000004458 analytical method Methods 0.000 description 1
239000013060 biological fluid Substances 0.000 description 1
239000012620 biological material Substances 0.000 description 1
239000000337 buffer salt Substances 0.000 description 1
238000004364 calculation method Methods 0.000 description 1
239000012876 carrier material Substances 0.000 description 1
150000001768 cations Chemical class 0.000 description 1
KXGVEGMKQFWNSR-LLQZFEROSA-N deoxycholic acid Chemical compound C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 KXGVEGMKQFWNSR-LLQZFEROSA-N 0.000 description 1
229960003964 deoxycholic acid Drugs 0.000 description 1
KXGVEGMKQFWNSR-UHFFFAOYSA-N deoxycholic acid Natural products C1CC2CC(O)CCC2(C)C2C1C1CCC(C(CCC(O)=O)C)C1(C)C(O)C2 KXGVEGMKQFWNSR-UHFFFAOYSA-N 0.000 description 1
238000013461 design Methods 0.000 description 1
WOWBFOBYOAGEEA-UHFFFAOYSA-N diafenthiuron Chemical compound CC(C)C1=C(NC(=S)NC(C)(C)C)C(C(C)C)=CC(OC=2C=CC=CC=2)=C1 WOWBFOBYOAGEEA-UHFFFAOYSA-N 0.000 description 1
238000003745 diagnosis Methods 0.000 description 1
238000003748 differential diagnosis Methods 0.000 description 1
XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 description 1
235000011180 diphosphates Nutrition 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
230000002255 enzymatic effect Effects 0.000 description 1
238000006911 enzymatic reaction Methods 0.000 description 1
150000002148 esters Chemical class 0.000 description 1
150000002170 ethers Chemical class 0.000 description 1
229940065734 gamma-aminobutyrate Drugs 0.000 description 1
235000013922 glutamic acid Nutrition 0.000 description 1
239000004220 glutamic acid Substances 0.000 description 1
HHLFWLYXYJOTON-UHFFFAOYSA-N glyoxylic acid Chemical compound OC(=O)C=O HHLFWLYXYJOTON-UHFFFAOYSA-N 0.000 description 1
210000002216 heart Anatomy 0.000 description 1
208000019622 heart disease Diseases 0.000 description 1
238000005470 impregnation Methods 0.000 description 1
238000010348 incorporation Methods 0.000 description 1
239000004615 ingredient Substances 0.000 description 1
230000002452 interceptive effect Effects 0.000 description 1
125000000468 ketone group Chemical group 0.000 description 1
210000004185 liver Anatomy 0.000 description 1
208000019423 liver disease Diseases 0.000 description 1
159000000003 magnesium salts Chemical class 0.000 description 1
229940049920 malate Drugs 0.000 description 1
BJEPYKJPYRNKOW-UHFFFAOYSA-N malic acid Chemical compound OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 1
238000004519 manufacturing process Methods 0.000 description 1
230000036651 mood Effects 0.000 description 1
229940049964 oleate Drugs 0.000 description 1
ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 1
230000003287 optical effect Effects 0.000 description 1
230000001575 pathological effect Effects 0.000 description 1
239000008363 phosphate buffer Substances 0.000 description 1
210000002381 plasma Anatomy 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
230000002035 prolonged effect Effects 0.000 description 1
239000001397 quillaja saponaria molina bark Substances 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
239000012488 sample solution Substances 0.000 description 1
229930182490 saponin Natural products 0.000 description 1
150000007949 saponins Chemical class 0.000 description 1
229920006395 saturated elastomer Polymers 0.000 description 1
239000000344 soap Substances 0.000 description 1
KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 1
238000010998 test method Methods 0.000 description 1
239000012085 test solution Substances 0.000 description 1
210000001519 tissue Anatomy 0.000 description 1
231100000331 toxic Toxicity 0.000 description 1
230000002588 toxic effect Effects 0.000 description 1
238000012546 transfer Methods 0.000 description 1
GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
210000002700 urine Anatomy 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/52—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving transaminase
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/805—Test papers

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Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Microbiology (AREA)
Immunology (AREA)
Physics & Mathematics (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
Biophysics (AREA)
Analytical Chemistry (AREA)
Biochemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

DD79214827A 1978-08-08 1979-08-06 Verfahren zur bestimmung von glutamat-oxalacetat-transaminase glutamat-pyruvat-transaminase DD145326A5 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
DE19782834706 DE2834706A1 (de)	1978-08-08	1978-08-08	Verfahren und reagens zur bestimmung von glutamat-oxalacetat-transaminase und glutamat-pyruvat-transaminase

Publications (1)

Publication Number	Publication Date
DD145326A5 true DD145326A5 (de)	1980-12-03

Family

ID=6046495

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DD79214827A DD145326A5 (de)	1978-08-08	1979-08-06	Verfahren zur bestimmung von glutamat-oxalacetat-transaminase glutamat-pyruvat-transaminase

Country Status (11)

Country	Link
US (1)	US4271265A (da)
EP (1)	EP0008342B1 (da)
JP (1)	JPS584918B2 (da)
AT (1)	AT362530B (da)
AU (1)	AU512662B2 (da)
CA (1)	CA1129314A (da)
DD (1)	DD145326A5 (da)
DE (2)	DE2834706A1 (da)
DK (1)	DK151974C (da)
SU (1)	SU1276269A3 (da)
YU (1)	YU192979A (da)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS5768798A (en)	1980-10-14	1982-04-27	Toyo Jozo Co Ltd	Novel measurement of amylase activity
DE3048662A1 (de) *	1980-12-23	1982-07-22	Boehringer Mannheim Gmbh, 6800 Mannheim	Stabilisierte zubereitung von tetrazoliumsalzen
DE3221730A1 (de) *	1982-06-09	1983-12-15	Boehringer Mannheim Gmbh, 6800 Mannheim	Verfahren und analytische mittel zur aktivitaetsbestimmung von glutamat-oxalacetat-transaminase
US4575488A (en) *	1982-06-25	1986-03-11	Technicon Instruments Corp.	Interference free transaminase assay
WO1984000779A1 (en) *	1982-08-09	1984-03-01	Eastman Kodak Co	Method for performing rate assays
DE3247894A1 (de) *	1982-12-24	1984-06-28	Merck Patent Gmbh, 6100 Darmstadt	Testsystem und verfahren zur bestimmung von nad(p)h
CA1218704A (en) *	1983-05-05	1987-03-03	Graham Davis	Assay systems using more than one enzyme
US5501949A (en) *	1985-12-10	1996-03-26	Murex Diagnostics Corporation	Particle bound binding component immunoassay
EP0231476A1 (en) *	1985-12-23	1987-08-12	Siddiqi, Iqbal W., Dr.	Selectively ion-permeable electrodes for analyzing selected ions in aqueous solution
AU6677386A (en) *	1985-12-23	1987-06-25	F. Hoffmann-La Roche Ag	Method of determining nadh or nadph
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US3206376A (en) *	1964-05-22	1965-09-14	Warner Lambert Pharmaceutical	Method of determining glutamic-oxalacetic transaminase
BE786291A (fr) *	1971-07-20	1973-01-15	Technicon Instr	Compositions de diagnostic pour determination de la glutamate-oxalate-transaminase (got) et de la glutamate-pyruvate-transaminase (gpt)
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1978
- 1978-08-08 DE DE19782834706 patent/DE2834706A1/de not_active Withdrawn
1979
- 1979-06-13 AT AT422979A patent/AT362530B/de not_active IP Right Cessation
- 1979-07-03 EP EP79102249A patent/EP0008342B1/de not_active Expired
- 1979-07-03 DE DE7979102249T patent/DE2961235D1/de not_active Expired
- 1979-07-11 CA CA331,592A patent/CA1129314A/en not_active Expired
- 1979-07-19 DK DK304079A patent/DK151974C/da not_active IP Right Cessation
- 1979-07-20 US US06/059,368 patent/US4271265A/en not_active Expired - Lifetime
- 1979-07-26 AU AU49277/79A patent/AU512662B2/en not_active Ceased
- 1979-08-06 SU SU792793851A patent/SU1276269A3/ru active
- 1979-08-06 DD DD79214827A patent/DD145326A5/de unknown
- 1979-08-07 YU YU01929/79A patent/YU192979A/xx unknown
- 1979-08-08 JP JP54100320A patent/JPS584918B2/ja not_active Expired

Also Published As

Publication number	Publication date
ATA422979A (de)	1980-10-15
EP0008342A1 (de)	1980-03-05
CA1129314A (en)	1982-08-10
DE2834706A1 (de)	1980-02-14
DK151974C (da)	1988-06-20
JPS584918B2 (ja)	1983-01-28
AT362530B (de)	1981-05-25
DK151974B (da)	1988-01-18
AU4927779A (en)	1980-02-14
YU192979A (en)	1984-04-30
US4271265A (en)	1981-06-02
EP0008342B1 (de)	1981-11-04
AU512662B2 (en)	1980-10-23
JPS5526894A (en)	1980-02-26
SU1276269A3 (ru)	1986-12-07
DK304079A (da)	1980-02-09
DE2961235D1 (en)	1982-01-14

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