DD150222A5 - Verfahren und reagenz zur aktivierung der cholesterinesterase - Google Patents

Verfahren und reagenz zur aktivierung der cholesterinesterase Download PDF

Info

Publication number: DD150222A5
Authority: DD; German Democratic Republic
Prior art keywords: ext; minutes; addition; detergent; carbon atoms
Prior art date: 1979-03-22

Application number

DD80219760A

Other languages

German (de)

English (en)

Inventor

Sigmar Klose

Herbert Buschek

Helmut Schlumberger

Original Assignee

Boehringer Mannheim Gmbh

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1979-03-22

Filing date

1980-03-19

Publication date

1981-08-19

1980-03-19 Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh

1981-08-19 Publication of DD150222A5 publication Critical patent/DD150222A5/de

Links

230000003213 activating effect Effects 0.000 title claims abstract description 30
238000000034 method Methods 0.000 title claims abstract description 15
239000003153 chemical reaction reagent Substances 0.000 title description 5
108010055297 Sterol Esterase Proteins 0.000 claims abstract description 44
102000000019 Sterol Esterase Human genes 0.000 claims abstract description 44
-1 bile acid compound Chemical class 0.000 claims abstract description 38
125000004432 carbon atom Chemical group C* 0.000 claims abstract description 17
RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims abstract description 16
235000014113 dietary fatty acids Nutrition 0.000 claims abstract description 8
239000000194 fatty acid Substances 0.000 claims abstract description 8
229930195729 fatty acid Natural products 0.000 claims abstract description 8
150000003839 salts Chemical class 0.000 claims abstract description 8
239000004094 surface-active agent Substances 0.000 claims abstract description 8
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 7
239000003613 bile acid Substances 0.000 claims abstract description 7
150000002148 esters Chemical class 0.000 claims abstract description 7
150000004665 fatty acids Chemical class 0.000 claims abstract description 5
125000005843 halogen group Chemical group 0.000 claims abstract description 4
230000002195 synergetic effect Effects 0.000 claims abstract description 3
239000003599 detergent Substances 0.000 claims description 97
102000004190 Enzymes Human genes 0.000 claims description 24
108090000790 Enzymes Proteins 0.000 claims description 24
239000000203 mixture Substances 0.000 claims description 9
KXGVEGMKQFWNSR-UHFFFAOYSA-N deoxycholic acid Natural products C1CC2CC(O)CCC2(C)C2C1C1CCC(C(CCC(O)=O)C)C1(C)C(O)C2 KXGVEGMKQFWNSR-UHFFFAOYSA-N 0.000 claims description 6
239000003795 chemical substances by application Substances 0.000 claims description 5
BHQCQFFYRZLCQQ-UHFFFAOYSA-N (3alpha,5alpha,7alpha,12alpha)-3,7,12-trihydroxy-cholan-24-oic acid Natural products OC1CC2CC(O)CCC2(C)C2C1C1CCC(C(CCC(O)=O)C)C1(C)C(O)C2 BHQCQFFYRZLCQQ-UHFFFAOYSA-N 0.000 claims description 4
239000004380 Cholic acid Substances 0.000 claims description 4
BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 claims description 4
235000019416 cholic acid Nutrition 0.000 claims description 4
229960002471 cholic acid Drugs 0.000 claims description 4
KXGVEGMKQFWNSR-LLQZFEROSA-N deoxycholic acid Chemical compound C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 KXGVEGMKQFWNSR-LLQZFEROSA-N 0.000 claims description 2
229960003964 deoxycholic acid Drugs 0.000 claims description 2
229910052783 alkali metal Inorganic materials 0.000 claims 2
239000011541 reaction mixture Substances 0.000 abstract description 13
238000006911 enzymatic reaction Methods 0.000 abstract description 2
229910052736 halogen Inorganic materials 0.000 abstract 1
238000007792 addition Methods 0.000 description 83
239000000654 additive Substances 0.000 description 51
230000000996 additive effect Effects 0.000 description 46
230000000694 effects Effects 0.000 description 37
239000000872 buffer Substances 0.000 description 30
239000002671 adjuvant Substances 0.000 description 22
238000006243 chemical reaction Methods 0.000 description 21
HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 20
MSXVEPNJUHWQHW-UHFFFAOYSA-N 2-methylbutan-2-ol Chemical compound CCC(C)(C)O MSXVEPNJUHWQHW-UHFFFAOYSA-N 0.000 description 16
239000008363 phosphate buffer Substances 0.000 description 15
PCWGTDULNUVNBN-UHFFFAOYSA-N 4-methylpentan-1-ol Chemical compound CC(C)CCCO PCWGTDULNUVNBN-UHFFFAOYSA-N 0.000 description 13
HPXRVTGHNJAIIH-UHFFFAOYSA-N cyclohexanol Chemical compound OC1CCCCC1 HPXRVTGHNJAIIH-UHFFFAOYSA-N 0.000 description 13
KPWDGTGXUYRARH-UHFFFAOYSA-N 2,2,2-trichloroethanol Chemical compound OCC(Cl)(Cl)Cl KPWDGTGXUYRARH-UHFFFAOYSA-N 0.000 description 11
WWZKQHOCKIZLMA-UHFFFAOYSA-M octanoate Chemical compound CCCCCCCC([O-])=O WWZKQHOCKIZLMA-UHFFFAOYSA-M 0.000 description 11
239000000243 solution Substances 0.000 description 11
235000012000 cholesterol Nutrition 0.000 description 10
229910052760 oxygen Inorganic materials 0.000 description 10
239000008057 potassium phosphate buffer Substances 0.000 description 8
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 7
RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 description 6
239000007983 Tris buffer Substances 0.000 description 6
230000004913 activation Effects 0.000 description 6
150000001298 alcohols Chemical class 0.000 description 6
238000007257 deesterification reaction Methods 0.000 description 6
229920004890 Triton X-100 Polymers 0.000 description 5
239000013504 Triton X-100 Substances 0.000 description 5
239000007853 buffer solution Substances 0.000 description 5
230000000052 comparative effect Effects 0.000 description 5
230000001419 dependent effect Effects 0.000 description 5
239000000126 substance Substances 0.000 description 5
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 5
WDNBURPWRNALGP-UHFFFAOYSA-N 3,4-Dichlorophenol Chemical compound OC1=CC=C(Cl)C(Cl)=C1 WDNBURPWRNALGP-UHFFFAOYSA-N 0.000 description 4
FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 4
108090000371 Esterases Proteins 0.000 description 4
AMQJEAYHLZJPGS-UHFFFAOYSA-N N-Pentanol Chemical compound CCCCCO AMQJEAYHLZJPGS-UHFFFAOYSA-N 0.000 description 4
150000002170 ethers Chemical class 0.000 description 4
238000005259 measurement Methods 0.000 description 4
239000007787 solid Substances 0.000 description 4
239000000758 substrate Substances 0.000 description 4
229920003171 Poly (ethylene oxide) Polymers 0.000 description 3
229920001213 Polysorbate 20 Polymers 0.000 description 3
FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 3
238000004458 analytical method Methods 0.000 description 3
238000013459 approach Methods 0.000 description 3
230000005764 inhibitory process Effects 0.000 description 3
239000007788 liquid Substances 0.000 description 3
125000006353 oxyethylene group Chemical group 0.000 description 3
239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 3
235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 3
235000002906 tartaric acid Nutrition 0.000 description 3
239000011975 tartaric acid Substances 0.000 description 3
KBPLFHHGFOOTCA-UHFFFAOYSA-N 1-Octanol Chemical compound CCCCCCCCO KBPLFHHGFOOTCA-UHFFFAOYSA-N 0.000 description 2
IEQAICDLOKRSRL-UHFFFAOYSA-N 2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-(2-dodecoxyethoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethanol Chemical compound CCCCCCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO IEQAICDLOKRSRL-UHFFFAOYSA-N 0.000 description 2
VPOMSPZBQMDLTM-UHFFFAOYSA-N 3,5-dichlorophenol Chemical compound OC1=CC(Cl)=CC(Cl)=C1 VPOMSPZBQMDLTM-UHFFFAOYSA-N 0.000 description 2
BUADUHVXMFJVLH-UHFFFAOYSA-N 7-chloro-3-imidazol-1-yl-2H-1,2,4-benzotriazin-1-ium 1-oxide Chemical compound N1[N+](=O)C2=CC(Cl)=CC=C2N=C1N1C=CN=C1 BUADUHVXMFJVLH-UHFFFAOYSA-N 0.000 description 2
ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 2
238000002835 absorbance Methods 0.000 description 2
150000001447 alkali salts Chemical class 0.000 description 2
125000000217 alkyl group Chemical group 0.000 description 2
229910052801 chlorine Inorganic materials 0.000 description 2
239000000460 chlorine Substances 0.000 description 2
150000001875 compounds Chemical class 0.000 description 2
JHIVVAPYMSGYDF-UHFFFAOYSA-N cyclohexanone Chemical compound O=C1CCCCC1 JHIVVAPYMSGYDF-UHFFFAOYSA-N 0.000 description 2
MWKFXSUHUHTGQN-UHFFFAOYSA-N decan-1-ol Chemical compound CCCCCCCCCCO MWKFXSUHUHTGQN-UHFFFAOYSA-N 0.000 description 2
LQZZUXJYWNFBMV-UHFFFAOYSA-N dodecan-1-ol Chemical compound CCCCCCCCCCCCO LQZZUXJYWNFBMV-UHFFFAOYSA-N 0.000 description 2
238000002474 experimental method Methods 0.000 description 2
150000002191 fatty alcohols Chemical class 0.000 description 2
ZSIAUFGUXNUGDI-UHFFFAOYSA-N hexan-1-ol Chemical compound CCCCCCO ZSIAUFGUXNUGDI-UHFFFAOYSA-N 0.000 description 2
150000002500 ions Chemical class 0.000 description 2
238000005580 one pot reaction Methods 0.000 description 2
238000005457 optimization Methods 0.000 description 2
229920000151 polyglycol Polymers 0.000 description 2
239000010695 polyglycol Substances 0.000 description 2
210000002966 serum Anatomy 0.000 description 2
FHHPUSMSKHSNKW-SMOYURAASA-M sodium deoxycholate Chemical compound [Na+].C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC([O-])=O)C)[C@@]2(C)[C@@H](O)C1 FHHPUSMSKHSNKW-SMOYURAASA-M 0.000 description 2
238000012360 testing method Methods 0.000 description 2
HSINOMROUCMIEA-FGVHQWLLSA-N (2s,4r)-4-[(3r,5s,6r,7r,8s,9s,10s,13r,14s,17r)-6-ethyl-3,7-dihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthren-17-yl]-2-methylpentanoic acid Chemical class C([C@@]12C)C[C@@H](O)C[C@H]1[C@@H](CC)[C@@H](O)[C@@H]1[C@@H]2CC[C@]2(C)[C@@H]([C@H](C)C[C@H](C)C(O)=O)CC[C@H]21 HSINOMROUCMIEA-FGVHQWLLSA-N 0.000 description 1
XELSBTMMZRMLAS-UHFFFAOYSA-N 1,1,2,2-tetrachloropropan-1-ol Chemical compound CC(Cl)(Cl)C(O)(Cl)Cl XELSBTMMZRMLAS-UHFFFAOYSA-N 0.000 description 1
HSQFVBWFPBKHEB-UHFFFAOYSA-N 2,3,4-trichlorophenol Chemical class OC1=CC=C(Cl)C(Cl)=C1Cl HSQFVBWFPBKHEB-UHFFFAOYSA-N 0.000 description 1
QSDORQFGFBJHTO-UHFFFAOYSA-N 3,3,3-trichloropropan-1-ol Chemical compound OCCC(Cl)(Cl)Cl QSDORQFGFBJHTO-UHFFFAOYSA-N 0.000 description 1
BWDBEAQIHAEVLV-UHFFFAOYSA-N 6-methylheptan-1-ol Chemical compound CC(C)CCCCCO BWDBEAQIHAEVLV-UHFFFAOYSA-N 0.000 description 1
WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 description 1
108010089254 Cholesterol oxidase Proteins 0.000 description 1
PXGOKWXKJXAPGV-UHFFFAOYSA-N Fluorine Chemical compound FF PXGOKWXKJXAPGV-UHFFFAOYSA-N 0.000 description 1
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
101710132772 Peroxidase 1 Proteins 0.000 description 1
102000003992 Peroxidases Human genes 0.000 description 1
229920001214 Polysorbate 60 Polymers 0.000 description 1
241000270295 Serpentes Species 0.000 description 1
PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
CIUQDSCDWFSTQR-UHFFFAOYSA-N [C]1=CC=CC=C1 Chemical compound [C]1=CC=CC=C1 CIUQDSCDWFSTQR-UHFFFAOYSA-N 0.000 description 1
239000002253 acid Substances 0.000 description 1
230000002411 adverse Effects 0.000 description 1
150000001346 alkyl aryl ethers Chemical class 0.000 description 1
125000002877 alkyl aryl group Chemical group 0.000 description 1
125000003710 aryl alkyl group Chemical group 0.000 description 1
125000003118 aryl group Chemical group 0.000 description 1
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 1
229910052794 bromium Inorganic materials 0.000 description 1
230000008859 change Effects 0.000 description 1
GGCLNOIGPMGLDB-GYKMGIIDSA-N cholest-5-en-3-one Chemical compound C1C=C2CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 GGCLNOIGPMGLDB-GYKMGIIDSA-N 0.000 description 1
NYOXRYYXRWJDKP-UHFFFAOYSA-N cholestenone Natural products C1CC2=CC(=O)CCC2(C)C2C1C1CCC(C(C)CCCC(C)C)C1(C)CC2 NYOXRYYXRWJDKP-UHFFFAOYSA-N 0.000 description 1
150000001840 cholesterol esters Chemical class 0.000 description 1
238000013461 design Methods 0.000 description 1
230000001627 detrimental effect Effects 0.000 description 1
GJYVZUKSNFSLCL-UHFFFAOYSA-N dichloromethanol Chemical compound OC(Cl)Cl GJYVZUKSNFSLCL-UHFFFAOYSA-N 0.000 description 1
239000000975 dye Substances 0.000 description 1
230000002255 enzymatic effect Effects 0.000 description 1
229910052731 fluorine Inorganic materials 0.000 description 1
239000011737 fluorine Substances 0.000 description 1
235000013305 food Nutrition 0.000 description 1
238000009472 formulation Methods 0.000 description 1
239000011521 glass Substances 0.000 description 1
125000003827 glycol group Chemical group 0.000 description 1
150000002367 halogens Chemical group 0.000 description 1
230000003993 interaction Effects 0.000 description 1
210000004185 liver Anatomy 0.000 description 1
QXLPXWSKPNOQLE-UHFFFAOYSA-N methylpentynol Chemical compound CCC(C)(O)C#C QXLPXWSKPNOQLE-UHFFFAOYSA-N 0.000 description 1
244000005700 microbiome Species 0.000 description 1
NCIAGQNZQHYKGR-UHFFFAOYSA-N naphthalene-1,2,3-triol Chemical compound C1=CC=C2C(O)=C(O)C(O)=CC2=C1 NCIAGQNZQHYKGR-UHFFFAOYSA-N 0.000 description 1
ZUVBIBLYOCVYJU-UHFFFAOYSA-N naphthalene-1,7-diol Chemical compound C1=CC=C(O)C2=CC(O)=CC=C21 ZUVBIBLYOCVYJU-UHFFFAOYSA-N 0.000 description 1
150000004780 naphthols Chemical class 0.000 description 1
229920002114 octoxynol-9 Polymers 0.000 description 1
239000001301 oxygen Substances 0.000 description 1
108040007629 peroxidase activity proteins Proteins 0.000 description 1
239000000575 pesticide Substances 0.000 description 1
150000002989 phenols Chemical class 0.000 description 1
238000005375 photometry Methods 0.000 description 1
229920000259 polyoxyethylene lauryl ether Polymers 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
230000008569 process Effects 0.000 description 1
239000000376 reactant Substances 0.000 description 1
230000035484 reaction time Effects 0.000 description 1
239000001044 red dye Substances 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
238000000926 separation method Methods 0.000 description 1
239000011734 sodium Substances 0.000 description 1
229910052708 sodium Inorganic materials 0.000 description 1
229960005480 sodium caprylate Drugs 0.000 description 1
BYKRNSHANADUFY-UHFFFAOYSA-M sodium octanoate Chemical compound [Na+].CCCCCCCC([O-])=O BYKRNSHANADUFY-UHFFFAOYSA-M 0.000 description 1
159000000000 sodium salts Chemical class 0.000 description 1
229910052938 sodium sulfate Inorganic materials 0.000 description 1
235000011152 sodium sulphate Nutrition 0.000 description 1
239000011550 stock solution Substances 0.000 description 1
239000011885 synergistic combination Substances 0.000 description 1
GYLIOGDFGLKMOL-UHFFFAOYSA-N trichloromethanol Chemical compound OC(Cl)(Cl)Cl GYLIOGDFGLKMOL-UHFFFAOYSA-N 0.000 description 1
GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Genetics & Genomics (AREA)
Bioinformatics & Cheminformatics (AREA)
Biochemistry (AREA)
Biotechnology (AREA)
Microbiology (AREA)
Molecular Biology (AREA)
General Health & Medical Sciences (AREA)
General Engineering & Computer Science (AREA)
Medicinal Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Biomedical Technology (AREA)
Analytical Chemistry (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
Immunology (AREA)
Detergent Compositions (AREA)
Enzymes And Modification Thereof (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

DD80219760A 1979-03-22 1980-03-19 Verfahren und reagenz zur aktivierung der cholesterinesterase DD150222A5 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
DE2911284A DE2911284C2 (de)	1979-03-22	1979-03-22	Verfahren und Reagenz zur Aktivierung der Cholesterinesterase

Publications (1)

Publication Number	Publication Date
DD150222A5 true DD150222A5 (de)	1981-08-19

Family

ID=6066139

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DD80219760A DD150222A5 (de)	1979-03-22	1980-03-19	Verfahren und reagenz zur aktivierung der cholesterinesterase

Country Status (12)

Country	Link
EP (1)	EP0016946A1 (da)
JP (1)	JPS55127987A (da)
AR (1)	AR220448A1 (da)
AU (1)	AU520246B2 (da)
CA (1)	CA1131110A (da)
DD (1)	DD150222A5 (da)
DE (1)	DE2911284C2 (da)
DK (1)	DK148658C (da)
FI (1)	FI70044C (da)
HU (1)	HU183078B (da)
IL (1)	IL59571A0 (da)
ZA (1)	ZA801653B (da)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE3046241A1 (de) *	1980-12-08	1982-07-15	Boehringer Mannheim Gmbh, 6800 Mannheim	Verfahren und reagenz zur bestimmung von cholesterin
DE3200274A1 (de) *	1982-01-07	1983-07-14	Boehringer Mannheim Gmbh, 6800 Mannheim	Verfahren zur stabilisierung waessriger loesungen von cholesterinesterase aus pseudomonaden
GB2154735B (en) *	1984-01-27	1987-07-15	Menarini Sas	Reagent for determining blood glucose content
EP0259521A1 (en) *	1986-09-10	1988-03-16	Akzo N.V.	Test reagent for amylase determination

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
GB1479994A (en) *	1974-03-04	1977-07-13	Abbott Lab	Single reagent for the enzymatic determination of cholesterol and method therefor
DE2612725C3 (de) *	1976-03-25	1979-05-03	Boehringer Mannheim Gmbh, 6800 Mannheim	Verfahren und Reagenz zur Bestimmung von Cholesterin
DE2816229C2 (de) *	1978-04-14	1983-11-10	Boehringer Mannheim Gmbh, 6800 Mannheim	Verfahren und Mittel zur Beseitigung von Trübungen

1979
- 1979-03-22 DE DE2911284A patent/DE2911284C2/de not_active Expired
1980
- 1980-02-20 EP EP80100840A patent/EP0016946A1/de not_active Withdrawn
- 1980-02-22 CA CA346,298A patent/CA1131110A/en not_active Expired
- 1980-03-05 AR AR280186A patent/AR220448A1/es active
- 1980-03-11 FI FI800743A patent/FI70044C/fi not_active IP Right Cessation
- 1980-03-11 AU AU56334/80A patent/AU520246B2/en not_active Expired
- 1980-03-11 IL IL59571A patent/IL59571A0/xx unknown
- 1980-03-19 DD DD80219760A patent/DD150222A5/de not_active IP Right Cessation
- 1980-03-20 DK DK120380A patent/DK148658C/da not_active IP Right Cessation
- 1980-03-21 ZA ZA00801653A patent/ZA801653B/xx unknown
- 1980-03-21 HU HU80681A patent/HU183078B/hu not_active IP Right Cessation
- 1980-03-21 JP JP3481580A patent/JPS55127987A/ja active Granted

Also Published As

Publication number	Publication date
DK120380A (da)	1980-09-23
ZA801653B (en)	1981-04-29
AU520246B2 (en)	1982-01-21
DE2911284C2 (de)	1982-01-28
IL59571A0 (en)	1980-06-30
DE2911284B1 (de)	1980-10-02
FI800743A7 (fi)	1980-09-23
CA1131110A (en)	1982-09-07
AU5633480A (en)	1980-09-25
FI70044B (fi)	1986-01-31
AR220448A1 (es)	1980-10-31
DK148658B (da)	1985-08-26
JPS5645586B2 (da)	1981-10-27
JPS55127987A (en)	1980-10-03
DK148658C (da)	1986-01-27
EP0016946A1 (de)	1980-10-15
FI70044C (fi)	1986-09-12
HU183078B (en)	1984-04-28

Legal Events

Date	Code	Title	Description
1996-05-09	ENJ	Ceased due to non-payment of renewal fee

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