DD153683A5 - Verfahren zur reinigung von interferon - Google Patents

Verfahren zur reinigung von interferon Download PDF

Info

Publication number: DD153683A5
Authority: DD; German Democratic Republic
Prior art keywords: derivatives; alkyl; radicals; point; acid
Prior art date: 1979-10-24

Application number

DD80224717A

Other languages

German (de)

English (en)

Inventor

Ulrich Menge

Michael Morr

Maria-Regina Kula

Kristin Anastassiadis

Original Assignee

Biotechnolog Forschung Gmbh

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1979-10-24

Filing date

1980-10-23

Publication date

1982-01-27

1980-10-23 Application filed by Biotechnolog Forschung Gmbh filed Critical Biotechnolog Forschung Gmbh

1982-01-27 Publication of DD153683A5 publication Critical patent/DD153683A5/de

Links

102000014150 Interferons Human genes 0.000 title claims abstract description 29
108010050904 Interferons Proteins 0.000 title claims abstract description 29
229940079322 interferon Drugs 0.000 title claims abstract description 27
238000000034 method Methods 0.000 title claims abstract description 25
238000004140 cleaning Methods 0.000 title description 2
229920000570 polyether Polymers 0.000 claims abstract description 12
238000000746 purification Methods 0.000 claims abstract description 5
239000002253 acid Substances 0.000 claims description 25
150000003839 salts Chemical group 0.000 claims description 17
125000002947 alkylene group Chemical group 0.000 claims description 14
125000000217 alkyl group Chemical group 0.000 claims description 9
-1 dextran modified dextran Chemical class 0.000 claims description 9
229920001515 polyalkylene glycol Polymers 0.000 claims description 9
239000004721 Polyphenylene oxide Substances 0.000 claims description 8
150000007513 acids Chemical class 0.000 claims description 8
239000007795 chemical reaction product Substances 0.000 claims description 8
239000002585 base Substances 0.000 claims description 7
229910052500 inorganic mineral Inorganic materials 0.000 claims description 7
150000002500 ions Chemical class 0.000 claims description 7
239000011707 mineral Substances 0.000 claims description 7
229920002307 Dextran Polymers 0.000 claims description 6
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 6
125000003545 alkoxy group Chemical group 0.000 claims description 6
229960002086 dextran Drugs 0.000 claims description 6
150000002334 glycols Chemical class 0.000 claims description 6
229910052783 alkali metal Inorganic materials 0.000 claims description 5
150000001450 anions Chemical class 0.000 claims description 5
150000001732 carboxylic acid derivatives Chemical class 0.000 claims description 5
229920001282 polysaccharide Polymers 0.000 claims description 5
239000005017 polysaccharide Substances 0.000 claims description 5
239000000047 product Substances 0.000 claims description 5
150000005846 sugar alcohols Polymers 0.000 claims description 5
150000004676 glycans Chemical class 0.000 claims description 4
238000006068 polycondensation reaction Methods 0.000 claims description 4
229910052708 sodium Inorganic materials 0.000 claims description 4
239000011734 sodium Substances 0.000 claims description 4
QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 claims description 3
239000004215 Carbon black (E152) Substances 0.000 claims description 3
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 3
239000001913 cellulose Substances 0.000 claims description 3
229920002678 cellulose Polymers 0.000 claims description 3
229930195733 hydrocarbon Natural products 0.000 claims description 3
IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 claims description 2
239000002879 Lewis base Substances 0.000 claims description 2
WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 claims description 2
229910019142 PO4 Inorganic materials 0.000 claims description 2
ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 2
150000001298 alcohols Chemical class 0.000 claims description 2
229960000633 dextran sulfate Drugs 0.000 claims description 2
ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 2
229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 2
235000019797 dipotassium phosphate Nutrition 0.000 claims description 2
125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 claims description 2
229910017053 inorganic salt Inorganic materials 0.000 claims description 2
150000007527 lewis bases Chemical class 0.000 claims description 2
229910052744 lithium Inorganic materials 0.000 claims description 2
125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims description 2
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 2
239000010452 phosphate Substances 0.000 claims description 2
229910052700 potassium Inorganic materials 0.000 claims description 2
239000011591 potassium Substances 0.000 claims description 2
159000000001 potassium salts Chemical class 0.000 claims description 2
YBYIRNPNPLQARY-UHFFFAOYSA-N 1H-indene Chemical compound C1=CC=C2CC=CC2=C1 YBYIRNPNPLQARY-UHFFFAOYSA-N 0.000 claims 2
XLLXMBCBJGATSP-UHFFFAOYSA-N 2-phenylethenol Chemical compound OC=CC1=CC=CC=C1 XLLXMBCBJGATSP-UHFFFAOYSA-N 0.000 claims 1
FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 claims 1
GOOHAUXETOMSMM-UHFFFAOYSA-N Propylene oxide Chemical compound CC1CO1 GOOHAUXETOMSMM-UHFFFAOYSA-N 0.000 claims 1
239000004280 Sodium formate Substances 0.000 claims 1
238000009795 derivation Methods 0.000 claims 1
150000002430 hydrocarbons Chemical class 0.000 claims 1
125000001841 imino group Chemical group [H]N=* 0.000 claims 1
229940047124 interferons Drugs 0.000 claims 1
229920000609 methyl cellulose Polymers 0.000 claims 1
239000001923 methylcellulose Substances 0.000 claims 1
JESXATFQYMPTNL-UHFFFAOYSA-N mono-hydroxyphenyl-ethylene Natural products OC1=CC=CC=C1C=C JESXATFQYMPTNL-UHFFFAOYSA-N 0.000 claims 1
229920001281 polyalkylene Polymers 0.000 claims 1
239000002243 precursor Substances 0.000 claims 1
ULWHHBHJGPPBCO-UHFFFAOYSA-N propane-1,1-diol Chemical compound CCC(O)O ULWHHBHJGPPBCO-UHFFFAOYSA-N 0.000 claims 1
HLBBKKJFGFRGMU-UHFFFAOYSA-M sodium formate Chemical compound [Na+].[O-]C=O HLBBKKJFGFRGMU-UHFFFAOYSA-M 0.000 claims 1
235000019254 sodium formate Nutrition 0.000 claims 1
229940095064 tartrate Drugs 0.000 claims 1
125000004432 carbon atom Chemical group C* 0.000 description 34
150000001875 compounds Chemical class 0.000 description 23
102000004169 proteins and genes Human genes 0.000 description 11
108090000623 proteins and genes Proteins 0.000 description 11
125000004430 oxygen atom Chemical group O* 0.000 description 10
238000002360 preparation method Methods 0.000 description 10
LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 9
NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 8
125000002887 hydroxy group Chemical group [H]O* 0.000 description 7
YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 6
ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 description 6
238000006243 chemical reaction Methods 0.000 description 6
229910052760 oxygen Inorganic materials 0.000 description 6
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 5
238000009826 distribution Methods 0.000 description 5
150000002148 esters Chemical class 0.000 description 5
239000001301 oxygen Substances 0.000 description 5
235000011007 phosphoric acid Nutrition 0.000 description 5
229910052698 phosphorus Inorganic materials 0.000 description 5
239000011574 phosphorus Substances 0.000 description 5
229910052717 sulfur Inorganic materials 0.000 description 5
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
239000002202 Polyethylene glycol Substances 0.000 description 4
DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 4
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
229910000147 aluminium phosphate Inorganic materials 0.000 description 4
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 4
229920001223 polyethylene glycol Polymers 0.000 description 4
239000002904 solvent Substances 0.000 description 4
125000004434 sulfur atom Chemical group 0.000 description 4
LWIHDJKSTIGBAC-UHFFFAOYSA-K tripotassium phosphate Chemical compound [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 4
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
WMFOQBRAJBCJND-UHFFFAOYSA-M Lithium hydroxide Chemical compound [Li+].[OH-] WMFOQBRAJBCJND-UHFFFAOYSA-M 0.000 description 3
150000001412 amines Chemical class 0.000 description 3
229910052801 chlorine Inorganic materials 0.000 description 3
125000001309 chloro group Chemical group Cl* 0.000 description 3
238000002474 experimental method Methods 0.000 description 3
TUJKJAMUKRIRHC-UHFFFAOYSA-N hydroxyl Chemical compound [OH] TUJKJAMUKRIRHC-UHFFFAOYSA-N 0.000 description 3
239000000203 mixture Substances 0.000 description 3
150000003015 phosphoric acid halides Chemical class 0.000 description 3
RWVGQQGBQSJDQV-UHFFFAOYSA-M sodium;3-[[4-[(e)-[4-(4-ethoxyanilino)phenyl]-[4-[ethyl-[(3-sulfonatophenyl)methyl]azaniumylidene]-2-methylcyclohexa-2,5-dien-1-ylidene]methyl]-n-ethyl-3-methylanilino]methyl]benzenesulfonate Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C(=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C)C=2C(=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C)C=C1 RWVGQQGBQSJDQV-UHFFFAOYSA-M 0.000 description 3
238000006467 substitution reaction Methods 0.000 description 3
QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
102000004190 Enzymes Human genes 0.000 description 2
108090000790 Enzymes Proteins 0.000 description 2
JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
150000001735 carboxylic acids Chemical class 0.000 description 2
125000004663 dialkyl amino group Chemical group 0.000 description 2
150000004820 halides Chemical class 0.000 description 2
125000005843 halogen group Chemical group 0.000 description 2
102000039446 nucleic acids Human genes 0.000 description 2
108020004707 nucleic acids Proteins 0.000 description 2
150000007523 nucleic acids Chemical class 0.000 description 2
VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 2
150000003008 phosphonic acid esters Chemical class 0.000 description 2
150000003009 phosphonic acids Chemical class 0.000 description 2
XHXFXVLFKHQFAL-UHFFFAOYSA-N phosphoryl trichloride Chemical compound ClP(Cl)(Cl)=O XHXFXVLFKHQFAL-UHFFFAOYSA-N 0.000 description 2
229910000160 potassium phosphate Inorganic materials 0.000 description 2
235000011009 potassium phosphates Nutrition 0.000 description 2
125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
238000007127 saponification reaction Methods 0.000 description 2
238000000926 separation method Methods 0.000 description 2
239000011780 sodium chloride Substances 0.000 description 2
239000012064 sodium phosphate buffer Substances 0.000 description 2
HVLLSGMXQDNUAL-UHFFFAOYSA-N triphenyl phosphite Chemical class C=1C=CC=CC=1OP(OC=1C=CC=CC=1)OC1=CC=CC=C1 HVLLSGMXQDNUAL-UHFFFAOYSA-N 0.000 description 2
VZPXDCIISFTYOM-UHFFFAOYSA-K trisodium;1-amino-4-[4-[[4-chloro-6-(3-sulfonatoanilino)-1,3,5-triazin-2-yl]amino]-3-sulfonatoanilino]-9,10-dioxoanthracene-2-sulfonate Chemical group [Na+].[Na+].[Na+].C1=2C(=O)C3=CC=CC=C3C(=O)C=2C(N)=C(S([O-])(=O)=O)C=C1NC(C=C1S([O-])(=O)=O)=CC=C1NC(N=1)=NC(Cl)=NC=1NC1=CC=CC(S([O-])(=O)=O)=C1 VZPXDCIISFTYOM-UHFFFAOYSA-K 0.000 description 2
CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 1
MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
BDAGIHXWWSANSR-UHFFFAOYSA-M Formate Chemical compound [O-]C=O BDAGIHXWWSANSR-UHFFFAOYSA-M 0.000 description 1
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
CPELXLSAUQHCOX-UHFFFAOYSA-N Hydrogen bromide Chemical compound Br CPELXLSAUQHCOX-UHFFFAOYSA-N 0.000 description 1
241000237852 Mollusca Species 0.000 description 1
KUGRPPRAQNPSQD-UHFFFAOYSA-N OOOOO Chemical compound OOOOO KUGRPPRAQNPSQD-UHFFFAOYSA-N 0.000 description 1
UIQWBVPFHHQZHH-UHFFFAOYSA-N OOOOOOOOOOOOOO Chemical compound OOOOOOOOOOOOOO UIQWBVPFHHQZHH-UHFFFAOYSA-N 0.000 description 1
229920002684 Sepharose Polymers 0.000 description 1
229930006000 Sucrose Natural products 0.000 description 1
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
CIUQDSCDWFSTQR-UHFFFAOYSA-N [C]1=CC=CC=C1 Chemical compound [C]1=CC=CC=C1 CIUQDSCDWFSTQR-UHFFFAOYSA-N 0.000 description 1
230000002378 acidificating effect Effects 0.000 description 1
150000001340 alkali metals Chemical class 0.000 description 1
125000003282 alkyl amino group Chemical group 0.000 description 1
230000029936 alkylation Effects 0.000 description 1
238000005804 alkylation reaction Methods 0.000 description 1
PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
150000001413 amino acids Chemical class 0.000 description 1
229910021529 ammonia Inorganic materials 0.000 description 1
239000012491 analyte Substances 0.000 description 1
239000012736 aqueous medium Substances 0.000 description 1
125000004429 atom Chemical group 0.000 description 1
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
125000004181 carboxyalkyl group Chemical group 0.000 description 1
150000001768 cations Chemical class 0.000 description 1
YKCWQPZFAFZLBI-UHFFFAOYSA-N cibacron blue Chemical compound C1=2C(=O)C3=CC=CC=C3C(=O)C=2C(N)=C(S(O)(=O)=O)C=C1NC(C=C1S(O)(=O)=O)=CC=C1NC(N=1)=NC(Cl)=NC=1NC1=CC=CC=C1S(O)(=O)=O YKCWQPZFAFZLBI-UHFFFAOYSA-N 0.000 description 1
230000007812 deficiency Effects 0.000 description 1
125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 description 1
230000000694 effects Effects 0.000 description 1
150000002170 ethers Chemical class 0.000 description 1
238000000605 extraction Methods 0.000 description 1
230000002349 favourable effect Effects 0.000 description 1
238000000855 fermentation Methods 0.000 description 1
230000004151 fermentation Effects 0.000 description 1
210000002950 fibroblast Anatomy 0.000 description 1
239000012847 fine chemical Substances 0.000 description 1
125000000524 functional group Chemical group 0.000 description 1
ZZUFCTLCJUWOSV-UHFFFAOYSA-N furosemide Chemical compound C1=C(Cl)C(S(=O)(=O)N)=CC(C(O)=O)=C1NCC1=CC=CO1 ZZUFCTLCJUWOSV-UHFFFAOYSA-N 0.000 description 1
239000000499 gel Substances 0.000 description 1
239000008103 glucose Substances 0.000 description 1
229910052736 halogen Inorganic materials 0.000 description 1
150000002367 halogens Chemical class 0.000 description 1
230000007062 hydrolysis Effects 0.000 description 1
238000006460 hydrolysis reaction Methods 0.000 description 1
230000001900 immune effect Effects 0.000 description 1
230000008676 import Effects 0.000 description 1
229910003002 lithium salt Inorganic materials 0.000 description 1
159000000002 lithium salts Chemical class 0.000 description 1
150000008146 mannosides Chemical class 0.000 description 1
238000004519 manufacturing process Methods 0.000 description 1
239000000463 material Substances 0.000 description 1
239000000155 melt Substances 0.000 description 1
125000000956 methoxy group Chemical group [H]C([H])([H])O* 0.000 description 1
239000002808 molecular sieve Substances 0.000 description 1
238000006386 neutralization reaction Methods 0.000 description 1
238000005192 partition Methods 0.000 description 1
125000001997 phenyl group Chemical class [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
125000002071 phenylalkoxy group Chemical group 0.000 description 1
150000003016 phosphoric acids Chemical class 0.000 description 1
OJMIONKXNSYLSR-UHFFFAOYSA-N phosphorous acid Chemical compound OP(O)O OJMIONKXNSYLSR-UHFFFAOYSA-N 0.000 description 1
229920000642 polymer Polymers 0.000 description 1
238000006116 polymerization reaction Methods 0.000 description 1
LJCNRYVRMXRIQR-OLXYHTOASA-L potassium sodium L-tartrate Chemical compound [Na+].[K+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O LJCNRYVRMXRIQR-OLXYHTOASA-L 0.000 description 1
229940074439 potassium sodium tartrate Drugs 0.000 description 1
239000013587 production medium Substances 0.000 description 1
125000002572 propoxy group Chemical group [*]OC([H])([H])C(C([H])([H])[H])([H])[H] 0.000 description 1
125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
239000002516 radical scavenger Substances 0.000 description 1
239000011541 reaction mixture Substances 0.000 description 1
238000001953 recrystallisation Methods 0.000 description 1
URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 description 1
239000001488 sodium phosphate Substances 0.000 description 1
229910000162 sodium phosphate Inorganic materials 0.000 description 1
235000011006 sodium potassium tartrate Nutrition 0.000 description 1
239000002195 soluble material Substances 0.000 description 1
239000000126 substance Substances 0.000 description 1
229960004793 sucrose Drugs 0.000 description 1
WQYSXVGEZYESBR-UHFFFAOYSA-N thiophosphoryl chloride Chemical compound ClP(Cl)(Cl)=S WQYSXVGEZYESBR-UHFFFAOYSA-N 0.000 description 1
238000004448 titration Methods 0.000 description 1
DQWPFSLDHJDLRL-UHFFFAOYSA-N triethyl phosphate Chemical compound CCOP(=O)(OCC)OCC DQWPFSLDHJDLRL-UHFFFAOYSA-N 0.000 description 1
RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
238000010626 work up procedure Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08G65/00—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
- C08G65/02—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule from cyclic ethers by opening of the heterocyclic ring
- C08G65/32—Polymers modified by chemical after-treatment
- C08G65/329—Polymers modified by chemical after-treatment with organic compounds
- C08G65/337—Polymers modified by chemical after-treatment with organic compounds containing other elements
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/06—Phosphorus compounds without P—C bonds
- C07F9/08—Esters of oxyacids of phosphorus
- C07F9/09—Esters of phosphoric acids
- C07F9/091—Esters of phosphoric acids with hydroxyalkyl compounds with further substituents on alkyl
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/06—Phosphorus compounds without P—C bonds
- C07F9/08—Esters of oxyacids of phosphorus
- C07F9/09—Esters of phosphoric acids
- C07F9/14—Esters of phosphoric acids containing P(=O)-halide groups
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/06—Phosphorus compounds without P—C bonds
- C07F9/16—Esters of thiophosphoric acids or thiophosphorous acids
- C07F9/165—Esters of thiophosphoric acids
- C07F9/1651—Esters of thiophosphoric acids with hydroxyalkyl compounds with further substituents on alkyl
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/06—Phosphorus compounds without P—C bonds
- C07F9/16—Esters of thiophosphoric acids or thiophosphorous acids
- C07F9/165—Esters of thiophosphoric acids
- C07F9/20—Esters of thiophosphoric acids containing P-halide groups
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08G65/00—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
- C08G65/02—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule from cyclic ethers by opening of the heterocyclic ring
- C08G65/32—Polymers modified by chemical after-treatment
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08G65/00—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
- C08G65/02—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule from cyclic ethers by opening of the heterocyclic ring
- C08G65/32—Polymers modified by chemical after-treatment
- C08G65/329—Polymers modified by chemical after-treatment with organic compounds
- C08G65/335—Polymers modified by chemical after-treatment with organic compounds containing phosphorus
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08G65/00—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
- C08G65/02—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule from cyclic ethers by opening of the heterocyclic ring
- C08G65/32—Polymers modified by chemical after-treatment
- C08G65/329—Polymers modified by chemical after-treatment with organic compounds
- C08G65/336—Polymers modified by chemical after-treatment with organic compounds containing silicon

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
General Health & Medical Sciences (AREA)
Medicinal Chemistry (AREA)
Polymers & Plastics (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Genetics & Genomics (AREA)
Zoology (AREA)
Gastroenterology & Hepatology (AREA)
Biophysics (AREA)
Toxicology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Peptides Or Proteins (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Medicinal Preparation (AREA)
Polyethers (AREA)

DD80224717A 1979-10-24 1980-10-23 Verfahren zur reinigung von interferon DD153683A5 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
DE2943016A DE2943016C2 (de)	1979-10-24	1979-10-24	Verfahren zur Reinigung von Interferon

Publications (1)

Publication Number	Publication Date
DD153683A5 true DD153683A5 (de)	1982-01-27

Family

ID=6084294

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DD80224717A DD153683A5 (de)	1979-10-24	1980-10-23	Verfahren zur reinigung von interferon

Country Status (8)

Country	Link
US (1)	US4343735A (da)
EP (1)	EP0028016B1 (da)
JP (1)	JPS5791924A (da)
AT (1)	ATE8898T1 (da)
DD (1)	DD153683A5 (da)
DE (2)	DE2943016C2 (da)
DK (1)	DK448280A (da)
IL (1)	IL61330A (da)

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WO1981000571A1 (fr) *	1979-08-30	1981-03-05	Biotechnolog Forschung Gmbh	Acides phosphoriques et leurs derives
SE8006102L (sv) *	1980-09-02	1982-03-03	Gambro Ab	Sett att utvinna en peptidinnehallande forening samt medel for genomforande av settet
US5702699A (en) *	1982-09-23	1997-12-30	Cetus Corporation	Process for the recovery of lipophilic proteins
US4462940A (en) *	1982-09-23	1984-07-31	Cetus Corporation	Process for the recovery of human β-interferon-like polypeptides
US4992271A (en) *	1982-09-23	1991-02-12	Cetus Corporation	Formulation for lipophilic IL-2 proteins
DE3421302A1 (de) *	1984-06-08	1985-12-12	Hoechst Ag, 6230 Frankfurt	Verfahren zur isolierung und reinigung von (alpha)-interferonen
US4690892A (en) *	1985-04-29	1987-09-01	Union Carbide Corporation	Process for the recycle of phase-forming liquids of multi-phase aqueous systems
US4743550A (en) *	1985-04-29	1988-05-10	Union Carbide Corporation	Method for improving the partition coefficient in enzyme-containing systems having at least two phases
US5183746A (en) *	1986-10-27	1993-02-02	Schering Aktiengesellschaft	Formulation processes for pharmaceutical compositions of recombinant β-
US5037644A (en) *	1986-10-27	1991-08-06	Cetus Corporation	Pharmaceutical compositions of recombinant interleukin-2 and formulation processes
US4894330A (en) *	1986-12-23	1990-01-16	Cetus Corporation	Purification of recombinant beta-interferon incorporating RP-HPLC
US5004605A (en) *	1987-12-10	1991-04-02	Cetus Corporation	Low pH pharmaceutical compositions of recombinant β-interferon
DE3908422A1 (de) *	1989-03-15	1990-09-20	Forschungszentrum Juelich Gmbh	Verfahren zur extraktion von proteinen aus waessrigen biomassesuspensionen
US5151358A (en) *	1989-06-13	1992-09-29	Genencor International, Inc.	Processes for the recovery of naturally produced chymosin
US5139943A (en) *	1989-06-13	1992-08-18	Genencor International, Inc.	Processes for the recovery of microbially produced chymosin
DE3932311A1 (de) *	1989-09-28	1991-04-11	Boehringer Ingelheim Int	Verfahren zur reinigung von beta-interferon
US5328841A (en) *	1990-10-05	1994-07-12	Genencor International, Inc.	Methods for isolating EG III cellulase component and EG III cellulase in polyethylene glycol using inorganic salt and polyethylene glycol
US5290474A (en) *	1990-10-05	1994-03-01	Genencor International, Inc.	Detergent composition for treating cotton-containing fabrics containing a surfactant and a cellulase composition containing endolucanase III from trichoderma ssp
CA2093422C (en) *	1990-10-05	2001-04-03	Genencor International, Inc.	DETERGENT COMPOSITIONS CONTAINING LOW CBH I CONTENT CELLULASE COMPOSITIONS
SE9003534D0 (sv) *	1990-11-06	1990-11-06	Kabigen Ab	A method for isolating and purifying peptides and proteins
US5407810A (en) *	1993-08-20	1995-04-18	Genentech, Inc.	Aqueous multiple-phase isolation of polypeptide
US6399296B1 (en) *	1994-07-20	2002-06-04	The General Hospital Corporation	Interaction trap systems for detecting protein interactions
UY27373A1 (es) *	2001-07-09	2003-02-28	Schering Ag	Formulaciones de interferón beta-humano
KR20080009111A (ko)	2005-04-11	2008-01-24	새비언트 파마수티컬즈 인크.	유레이트 옥시다아제의 변이형 및 이의 용도
KR101288027B1 (ko) *	2006-04-12	2013-07-22	새비언트 파마수티컬즈 인크.	양이온 계면활성제를 이용한 단백질 정제 방법
JP2012531596A (ja)	2009-06-25	2012-12-10	サヴィエント・ファーマシューティカルズ・インコーポレイテッド	Ｐｅｇ化ウリカーゼ療法における血清尿酸をモニタリングすることにより注入反応の危険性および抗体媒介性効果消失を予測するための方法およびキット
JP6488890B2 (ja) *	2015-06-03	2019-03-27	信越化学工業株式会社	フルオロオキシアルキレン基含有ポリマー変性ホスホン酸誘導体、該誘導体を含む表面処理剤、該表面処理剤で処理された物品及び光学物品
CN116637177A (zh)	2016-11-11	2023-08-25	好利恩治疗美国公司	泼尼松和尿酸酶分子的组合疗法及其用途
US12121566B2 (en)	2019-01-30	2024-10-22	Horizon Therapeutics Usa, Inc.	Methods for treating gout
WO2020160325A1 (en)	2019-01-30	2020-08-06	Horizon Pharma Rheumatology Llc	Reducing immunogenicity to pegloticase
US12269875B2 (en)	2023-08-03	2025-04-08	Jeff R. Peterson	Gout flare prevention methods using IL-1BETA blockers

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Publication number	Priority date	Publication date	Assignee	Title
US3144390A (en) *	1960-06-28	1964-08-11	Nat Res Dev	Process for the purification of interferon
US3256152A (en) *	1965-06-14	1966-06-14	Merck & Co Inc	Concentration and purification of interferons, viral inhibiting substances (vis), viral inhibiting factors (vif), or viral inhibitory material
FR2252351A2 (en) *	1973-11-28	1975-06-20	Air Liquide	Isolation of enzymes by selective extraction - partition of polyinhibitor-enzyme complex between water and polyethylene glycol
DE2639129C3 (de) *	1976-08-31	1979-10-04	Gesellschaft Fuer Biotechnologische Forschung Mbh (Gbf), 3300 Braunschweig	Verfahren zur Abtrennung von Enzymen

1979
- 1979-10-24 DE DE2943016A patent/DE2943016C2/de not_active Expired
1980
- 1980-10-23 DE DE8080106495T patent/DE3068893D1/de not_active Expired
- 1980-10-23 US US06/200,015 patent/US4343735A/en not_active Expired - Lifetime
- 1980-10-23 AT AT80106495T patent/ATE8898T1/de active
- 1980-10-23 EP EP80106495A patent/EP0028016B1/de not_active Expired
- 1980-10-23 DD DD80224717A patent/DD153683A5/de unknown
- 1980-10-23 DK DK448280A patent/DK448280A/da not_active Application Discontinuation
- 1980-10-23 IL IL61330A patent/IL61330A/xx not_active IP Right Cessation
- 1980-10-24 JP JP55148267A patent/JPS5791924A/ja active Granted

Also Published As

Publication number	Publication date
DK448280A (da)	1981-04-25
JPH0223560B2 (da)	1990-05-24
ATE8898T1 (de)	1984-08-15
US4343735A (en)	1982-08-10
EP0028016A2 (de)	1981-05-06
JPS5791924A (en)	1982-06-08
IL61330A0 (en)	1980-12-31
EP0028016A3 (en)	1981-12-16
DE2943016C2 (de)	1984-09-06
DE2943016A1 (de)	1981-05-07
DE3068893D1 (en)	1984-09-13
EP0028016B1 (de)	1984-08-08
IL61330A (en)	1983-12-30

Publication	Publication Date	Title
DE2943016A1 (de)	1981-05-07	Verfahren zur reinigung von interferon
EP0175315B1 (de)	1989-01-11	4-Dimethylamino-1-hydroxybutan-1,1-diphosphonsäure, deren wasserlösliche Salze, Verfahren zu ihrer Herstellung sowie ihre Verwendung
DE2745083A1 (de)	1979-04-12	Hydroxydiphosphonsaeuren
EP0035027B1 (de)	1985-05-15	Neue säuren des phosphors und deren derivate
DE2061838B2 (de)	1976-04-29	2-phosphono-butan-1,2-dicarbonsaeure-derivate, verfahren zu ihrer herstellung und diese verbindungen enthaltenden mittel
DE855567C (de)	1952-11-13	Verfahren zur Herstellung von insektiziden Phosphorverbindungen
DE2949212C2 (da)	1989-02-16
DE753627C (de)	1951-01-29	Verfahren zur Darstellung von kernalkylierten Abkoemmlingen des Hydrochinons oder Naphthohydrochinons
DE1139611B (de)	1962-11-15	Keimtoetende, Jod enthaltende Praeparate
DE925230C (de)	1955-03-17	Verfahren zur Herstellung von Polyaethern der araliphatischen Reihe
DE19820974C2 (de)	2000-05-04	Isolierung der Salze von 1-Hydroxy-1,1-diphosphonsäureverbindungen
DE1249274B (de)	1967-09-07	Verfahren zur Herstellung von alkoholi sehe Hydroxylgruppen und Phosphor enthaltenden Polyathern, Zus z Änm C
DE942149C (de)	1956-04-26	Verfahren zur Herstellung substituierter Glycinamide
DE721667C (de)	1942-06-15	Verfahren zur Darstellung von Abkoemmlingen der 4-Aminobenzolsulfonamide
DE734957C (de)	1943-05-10	Verfahren zur Herstellung von Abkoemmlingen des p-Aminobenzolsulfonamids
DE69209258T2 (de)	1996-10-02	Verfahren zur Herstellung von Dichlormethylpyridinen
US2744902A (en)	1956-05-08	Alkyl naphthyl quaternary ammonium compounds
DE741891C (de)	1943-11-18	Verfahren zur Herstellung wasserloeslicher stickstoffhaltiger Kondensationsprodukte
DE1620675C (de)	1972-07-20	S-Benzoyloxymethylthiamine und Verfahren zu deren Herstellung
DE958834C (de)	1957-02-28	Verfahren zur Herstellung von reinen, durch saure Phosphatase leicht spaltbaren Phosphorsaeureestern des 4, 4'-Dioxydiaethylstilbens
DE532405C (de)	1931-08-27	Verfahren zur Darstellung von Arsenoverbindungen
DE1928985A1 (de)	1970-01-02	Antistatisches Mittel
DE715267C (de)	1941-12-18	Verfahren zur Herstellung von neutralen, oelloeslichen und sterilisierbaren organischen Wismutsalzen
DE1216471B (de)	1966-05-12	Waschmittel in fester oder fluessiger Form
DE2418677C3 (de)	1977-07-14	Fluorierte Phosphorsäure- a- und -betamonoester