DD202601A5 - Verfahren zu bestimmung von lecithin sowie reagens zur durchfuehrung dieses verfahrens - Google Patents

Verfahren zu bestimmung von lecithin sowie reagens zur durchfuehrung dieses verfahrens Download PDF

Info

Publication number: DD202601A5
Authority: DD; German Democratic Republic
Prior art keywords: mmol; lecithin; solution; borate; buffer
Prior art date: 1981-07-18

Application number

DD82241679A

Other languages

German (de)

English (en)

Inventor

Juergen Becker

Gerhard Beinstingl

Hans-Otto Beutler

Original Assignee

Boehringer Mannheim Gmbh

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1981-07-18

Filing date

1982-07-15

Publication date

1983-09-21

1982-07-15 Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh

1983-09-21 Publication of DD202601A5 publication Critical patent/DD202601A5/de

Links

IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 title claims abstract description 50
235000010445 lecithin Nutrition 0.000 title claims abstract description 50
229940067606 lecithin Drugs 0.000 title claims abstract description 50
239000000787 lecithin Substances 0.000 title claims abstract description 50
238000000034 method Methods 0.000 title claims abstract description 25
239000003153 chemical reaction reagent Substances 0.000 title claims description 15
239000000872 buffer Substances 0.000 claims abstract description 22
BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 claims abstract description 20
229910021538 borax Inorganic materials 0.000 claims abstract description 20
239000004328 sodium tetraborate Substances 0.000 claims abstract description 20
235000010339 sodium tetraborate Nutrition 0.000 claims abstract description 20
102000002260 Alkaline Phosphatase Human genes 0.000 claims abstract description 19
108020004774 Alkaline Phosphatase Proteins 0.000 claims abstract description 19
102000014384 Type C Phospholipases Human genes 0.000 claims abstract description 18
108010079194 Type C Phospholipases Proteins 0.000 claims abstract description 18
239000003599 detergent Substances 0.000 claims abstract description 17
ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 claims abstract description 15
102000002745 Choline Kinase Human genes 0.000 claims abstract description 12
108010018888 Choline kinase Proteins 0.000 claims abstract description 12
229960001231 choline Drugs 0.000 claims abstract description 10
OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 claims abstract description 10
YHHSONZFOIEMCP-UHFFFAOYSA-O phosphocholine Chemical compound C[N+](C)(C)CCOP(O)(O)=O YHHSONZFOIEMCP-UHFFFAOYSA-O 0.000 claims abstract description 8
229950004354 phosphorylcholine Drugs 0.000 claims abstract description 8
XTWYTFMLZFPYCI-KQYNXXCUSA-N 5'-adenylphosphoric acid Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XTWYTFMLZFPYCI-KQYNXXCUSA-N 0.000 claims abstract description 7
XTWYTFMLZFPYCI-UHFFFAOYSA-N Adenosine diphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(O)=O)C(O)C1O XTWYTFMLZFPYCI-UHFFFAOYSA-N 0.000 claims abstract description 7
NFAOATPOYUWEHM-UHFFFAOYSA-N 2-(6-methylheptyl)phenol Chemical compound CC(C)CCCCCC1=CC=CC=C1O NFAOATPOYUWEHM-UHFFFAOYSA-N 0.000 claims abstract description 6
239000002202 Polyethylene glycol Substances 0.000 claims abstract description 6
229920001223 polyethylene glycol Polymers 0.000 claims abstract description 6
ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 claims abstract description 5
239000007859 condensation product Substances 0.000 claims abstract description 5
239000000243 solution Substances 0.000 claims description 28
239000007853 buffer solution Substances 0.000 claims description 14
102000004190 Enzymes Human genes 0.000 claims description 13
108090000790 Enzymes Proteins 0.000 claims description 13
239000000725 suspension Substances 0.000 claims description 12
CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 10
BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 claims description 10
DTBNBXWJWCWCIK-UHFFFAOYSA-N phosphoenolpyruvic acid Chemical compound OC(=O)C(=C)OP(O)(O)=O DTBNBXWJWCWCIK-UHFFFAOYSA-N 0.000 claims description 10
238000003776 cleavage reaction Methods 0.000 claims description 9
229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 claims description 9
230000007017 scission Effects 0.000 claims description 9
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 8
102000003855 L-lactate dehydrogenase Human genes 0.000 claims description 8
108700023483 L-lactate dehydrogenases Proteins 0.000 claims description 8
102000013009 Pyruvate Kinase Human genes 0.000 claims description 8
108020005115 Pyruvate Kinase Proteins 0.000 claims description 8
239000008103 glucose Substances 0.000 claims description 8
229930029653 phosphoenolpyruvate Natural products 0.000 claims description 8
239000000470 constituent Substances 0.000 claims description 5
229910052943 magnesium sulfate Inorganic materials 0.000 claims description 5
235000019341 magnesium sulphate Nutrition 0.000 claims description 5
JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 claims description 4
239000005515 coenzyme Substances 0.000 claims description 4
229910001425 magnesium ion Inorganic materials 0.000 claims description 4
RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 claims 1
UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 12
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 12
239000000523 sample Substances 0.000 description 12
210000004381 amniotic fluid Anatomy 0.000 description 11
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
238000005259 measurement Methods 0.000 description 7
239000000203 mixture Substances 0.000 description 7
LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 6
230000008033 biological extinction Effects 0.000 description 6
238000006911 enzymatic reaction Methods 0.000 description 6
DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 4
210000004072 lung Anatomy 0.000 description 4
239000012074 organic phase Substances 0.000 description 4
230000035935 pregnancy Effects 0.000 description 4
239000012488 sample solution Substances 0.000 description 4
206010001052 Acute respiratory distress syndrome Diseases 0.000 description 3
208000013616 Respiratory Distress Syndrome Diseases 0.000 description 3
238000002835 absorbance Methods 0.000 description 3
238000006243 chemical reaction Methods 0.000 description 3
238000001514 detection method Methods 0.000 description 3
230000002255 enzymatic effect Effects 0.000 description 3
230000001605 fetal effect Effects 0.000 description 3
239000004094 surface-active agent Substances 0.000 description 3
239000002126 C01EB10 - Adenosine Substances 0.000 description 2
239000004471 Glycine Substances 0.000 description 2
LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 2
229960005305 adenosine Drugs 0.000 description 2
239000008346 aqueous phase Substances 0.000 description 2
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
238000001816 cooling Methods 0.000 description 2
210000002969 egg yolk Anatomy 0.000 description 2
235000013305 food Nutrition 0.000 description 2
159000000003 magnesium salts Chemical class 0.000 description 2
235000010746 mayonnaise Nutrition 0.000 description 2
239000008268 mayonnaise Substances 0.000 description 2
229950006238 nadide Drugs 0.000 description 2
150000003904 phospholipids Chemical class 0.000 description 2
KILNVBDSWZSGLL-UHFFFAOYSA-O 2-[2,3-di(hexadecanoyloxy)propoxy-hydroxyphosphoryl]oxyethyl-trimethylazanium Chemical compound CCCCCCCCCCCCCCCC(=O)OCC(COP(O)(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCCCCCCCCC KILNVBDSWZSGLL-UHFFFAOYSA-O 0.000 description 1
ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 description 1
JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 description 1
206010036590 Premature baby Diseases 0.000 description 1
ATBOMIWRCZXYSZ-XZBBILGWSA-N [1-[2,3-dihydroxypropoxy(hydroxy)phosphoryl]oxy-3-hexadecanoyloxypropan-2-yl] (9e,12e)-octadeca-9,12-dienoate Chemical compound CCCCCCCCCCCCCCCC(=O)OCC(COP(O)(=O)OCC(O)CO)OC(=O)CCCCCCC\C=C\C\C=C\CCCCC ATBOMIWRCZXYSZ-XZBBILGWSA-N 0.000 description 1
AWUCVROLDVIAJX-UHFFFAOYSA-N alpha-glycerophosphate Natural products OCC(O)COP(O)(O)=O AWUCVROLDVIAJX-UHFFFAOYSA-N 0.000 description 1
235000015173 baked goods and baking mixes Nutrition 0.000 description 1
150000001720 carbohydrates Chemical class 0.000 description 1
235000014633 carbohydrates Nutrition 0.000 description 1
238000005352 clarification Methods 0.000 description 1
238000003745 diagnosis Methods 0.000 description 1
239000001177 diphosphate Substances 0.000 description 1
239000012153 distilled water Substances 0.000 description 1
235000013345 egg yolk Nutrition 0.000 description 1
235000013601 eggs Nutrition 0.000 description 1
210000003754 fetus Anatomy 0.000 description 1
239000012530 fluid Substances 0.000 description 1
238000011534 incubation Methods 0.000 description 1
230000002401 inhibitory effect Effects 0.000 description 1
150000002632 lipids Chemical class 0.000 description 1
239000007788 liquid Substances 0.000 description 1
102000004169 proteins and genes Human genes 0.000 description 1
108090000623 proteins and genes Proteins 0.000 description 1
230000000241 respiratory effect Effects 0.000 description 1
239000006228 supernatant Substances 0.000 description 1
239000010409 thin film Substances 0.000 description 1
239000001226 triphosphate Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2405/00—Assays, e.g. immunoassays or enzyme assays, involving lipids
- G01N2405/04—Phospholipids, i.e. phosphoglycerides

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Microbiology (AREA)
Biochemistry (AREA)
Physics & Mathematics (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
Biophysics (AREA)
Analytical Chemistry (AREA)
Immunology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Investigating Or Analysing Biological Materials (AREA)
Analysing Materials By The Use Of Radiation (AREA)

DD82241679A 1981-07-18 1982-07-15 Verfahren zu bestimmung von lecithin sowie reagens zur durchfuehrung dieses verfahrens DD202601A5 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
DE19813128480 DE3128480A1 (de)	1981-07-18	1981-07-18	Verfahren zur bestimmung von lecithin in fruchtwasser sowie reagens zur durchfuehrung dieses verfahrens

Publications (1)

Publication Number	Publication Date
DD202601A5 true DD202601A5 (de)	1983-09-21

Family

ID=6137239

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DD82241679A DD202601A5 (de)	1981-07-18	1982-07-15	Verfahren zu bestimmung von lecithin sowie reagens zur durchfuehrung dieses verfahrens

Country Status (6)

Country	Link
EP (1)	EP0071076B1 (fr)
JP (1)	JPS5816698A (fr)
AT (1)	ATE13074T1 (fr)
CA (1)	CA1184101A (fr)
DD (1)	DD202601A5 (fr)
DE (2)	DE3128480A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS59193356A (ja) *	1983-03-17	1984-11-01	バイオウィッテッカー・インコーポレーテッド	全ＩｇＥ濃度の螢光光度分析及びそのための試薬
JPH061252B2 (ja) *	1984-08-31	1994-01-05	株式会社島津製作所	オキシダ−ゼ電極
JPS6351626A (ja) *	1986-08-20	1988-03-04	Nec Corp	半導体製造装置

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPS50142090A (fr) *	1974-04-30	1975-11-15
JPS5324888A (en) *	1976-08-19	1978-03-08	Kyowa Hakko Kogyo Kk	Composition for quantitating fats containing phosphorus
GB1555173A (en) *	1977-08-17	1979-11-07	Kyowa Hakko Kogyo Kk	Method and reagent for quantitative determination of phospholipids

1981
- 1981-07-18 DE DE19813128480 patent/DE3128480A1/de not_active Withdrawn
1982
- 1982-07-12 JP JP57119993A patent/JPS5816698A/ja active Granted
- 1982-07-14 AT AT82106278T patent/ATE13074T1/de active
- 1982-07-14 DE DE8282106278T patent/DE3263391D1/de not_active Expired
- 1982-07-14 EP EP82106278A patent/EP0071076B1/fr not_active Expired
- 1982-07-15 DD DD82241679A patent/DD202601A5/de unknown
- 1982-07-16 CA CA000407494A patent/CA1184101A/fr not_active Expired

Also Published As

Publication number	Publication date
CA1184101A (fr)	1985-03-19
EP0071076A3 (en)	1983-03-23
EP0071076B1 (fr)	1985-05-02
DE3263391D1 (en)	1985-06-05
ATE13074T1 (de)	1985-05-15
DE3128480A1 (de)	1983-01-27
JPS619839B2 (fr)	1986-03-26
JPS5816698A (ja)	1983-01-31
EP0071076A2 (fr)	1983-02-09

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DE2162325C3 (de)	1980-12-11	Verfahren zur Bestimmung von Fettsäureglycerinestern und Reagens zur Durchführung des Verfahrens
DE69130482T3 (de)	2006-05-04	Luciferase-zusammensetzungen und verfahren
EP0030718B1 (fr)	1982-12-01	Procédé et réactif pour la détermination des triglycérides
DE69032422T2 (de)	1999-02-04	Reagenzzusammensetzung, Verfahren und Kits zur Quantifizierung von Magnesium oder Calcium und Magnesium
DD145328A5 (de)	1980-12-03	Verfahren zur quantitativen enzymatischen bestimmung von adenosindiphosphat
DE2724758A1 (de)	1977-12-15	Zusammensetzung und verfahren zur hydrolyse von glycerinestern
DE2329174C2 (de)	1975-07-24	Verfahren zur quantitativen Bestimmung von Blutammoniak
EP0098562B1 (fr)	1986-03-05	Procédé pour la détermination de bilirubine conjuguée et totale ainsi que réactif propre à cette fin
DE2602961A1 (de)	1976-07-29	Verfahren zum nachweis und zur bestimmung eines reduzierten coenzyms
CH622555A5 (fr)	1981-04-15
DE1940816C3 (de)	1974-01-17	Verfahren und diagnostisches Mittel zur enzymatischen Bestimmung von Glucose
EP0120220A2 (fr)	1984-10-03	Méthode et réactif pour la détermination du glucose dans le sang hémolisé
DE2834706A1 (de)	1980-02-14	Verfahren und reagens zur bestimmung von glutamat-oxalacetat-transaminase und glutamat-pyruvat-transaminase
DE2512267A1 (de)	1975-09-25	Reagenz-komposition fuer die bestimmung von glycerin.
DD202601A5 (de)	1983-09-21	Verfahren zu bestimmung von lecithin sowie reagens zur durchfuehrung dieses verfahrens
DE1598067C3 (fr)	1974-04-18
EP0570356B1 (fr)	1999-02-17	Réactif pour la détermination du temps de thromboplastinee partiellement active (IIPa)
EP0018628B1 (fr)	1984-04-11	Procédé pour la détermination de la gamma-glutamyltranspeptidase
DE69030135T2 (de)	1997-06-19	Ein Reagenssystem zur Bestimmung von Lipase
DE2212285C3 (de)	1981-02-12	Verfahren zur Bestimmung der carcinogenen Aktivität und heptatoxischen Eigenschaften von Substanzen
DE3006789A1 (de)	1980-09-04	Verfahren und enzymzusammensetzung zur hydrolyse von glycerinestern
DE2823824A1 (de)	1978-12-07	Stabilisierte, fluessige, phosphat enthaltende masse fuer diagnosezwecke und verfahren zu ihrer herstellung
EP0020895A1 (fr)	1981-01-07	Thromboplastine partielle
DE3877565T2 (de)	1993-07-08	Kaltes stabiles fluessiges kreatininreagenz.
DE68918517T2 (de)	1995-05-04	Bestimmung des Ammoniakspiegels im Blut.