DD203777A5 - Verfahren zur bestimmung der aktivitaet von chymotrypsin oder trypsin im stuhl - Google Patents

Verfahren zur bestimmung der aktivitaet von chymotrypsin oder trypsin im stuhl Download PDF

Info

Publication number: DD203777A5
Authority: DD; German Democratic Republic
Prior art keywords: mmol; item; stool; items; substrate
Prior art date: 1981-10-06

Application number

DD82243765A

Other languages

German (de)

English (en)

Inventor

Gerald Moeller

Klaus P Kaspar

Original Assignee

Boehringer Mannheim Gmbh

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1981-10-06

Filing date

1982-10-04

Publication date

1983-11-02

1982-10-04 Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh

1983-11-02 Publication of DD203777A5 publication Critical patent/DD203777A5/de

Links

238000000034 method Methods 0.000 title claims abstract description 40
230000000694 effects Effects 0.000 title claims abstract description 17
239000000758 substrate Substances 0.000 claims abstract description 22
108090000317 Chymotrypsin Proteins 0.000 claims abstract description 20
229960002376 chymotrypsin Drugs 0.000 claims abstract description 20
239000000725 suspension Substances 0.000 claims abstract description 18
239000004094 surface-active agent Substances 0.000 claims abstract description 17
108090000631 Trypsin Proteins 0.000 claims abstract description 15
102000004142 Trypsin Human genes 0.000 claims abstract description 15
239000012588 trypsin Substances 0.000 claims abstract description 15
238000005259 measurement Methods 0.000 claims abstract description 14
239000000243 solution Substances 0.000 claims description 42
239000003153 chemical reaction reagent Substances 0.000 claims description 20
239000000872 buffer Substances 0.000 claims description 12
108090000790 Enzymes Proteins 0.000 claims description 10
102000004190 Enzymes Human genes 0.000 claims description 10
229940088598 enzyme Drugs 0.000 claims description 10
150000003839 salts Chemical class 0.000 claims description 9
238000005119 centrifugation Methods 0.000 claims description 7
238000003776 cleavage reaction Methods 0.000 claims description 7
230000007017 scission Effects 0.000 claims description 7
239000007983 Tris buffer Substances 0.000 claims description 6
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 6
150000001413 amino acids Chemical class 0.000 claims description 5
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
125000002091 cationic group Chemical group 0.000 claims description 3
239000003093 cationic surfactant Substances 0.000 claims description 3
239000002736 nonionic surfactant Substances 0.000 claims description 3
238000004448 titration Methods 0.000 claims description 3
150000003242 quaternary ammonium salts Chemical class 0.000 claims description 2
VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims 1
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 1
159000000007 calcium salts Chemical class 0.000 claims 1
239000011734 sodium Substances 0.000 claims 1
229910052708 sodium Inorganic materials 0.000 claims 1
239000006228 supernatant Substances 0.000 claims 1
210000003608 fece Anatomy 0.000 abstract 2
239000000523 sample Substances 0.000 description 23
239000003599 detergent Substances 0.000 description 14
238000002360 preparation method Methods 0.000 description 12
-1 example Chemical compound 0.000 description 9
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 7
238000000265 homogenisation Methods 0.000 description 7
HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 7
230000000052 comparative effect Effects 0.000 description 6
238000006243 chemical reaction Methods 0.000 description 5
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
239000007864 aqueous solution Substances 0.000 description 4
239000000470 constituent Substances 0.000 description 4
235000014113 dietary fatty acids Nutrition 0.000 description 4
DDXLVDQZPFLQMZ-UHFFFAOYSA-M dodecyl(trimethyl)azanium;chloride Chemical compound [Cl-].CCCCCCCCCCCC[N+](C)(C)C DDXLVDQZPFLQMZ-UHFFFAOYSA-M 0.000 description 4
239000000194 fatty acid Substances 0.000 description 4
229930195729 fatty acid Natural products 0.000 description 4
201000003883 Cystic fibrosis Diseases 0.000 description 3
239000002253 acid Substances 0.000 description 3
150000001412 amines Chemical class 0.000 description 3
229940024606 amino acid Drugs 0.000 description 3
125000004432 carbon atom Chemical group C* 0.000 description 3
RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 3
150000004665 fatty acids Chemical class 0.000 description 3
239000000203 mixture Substances 0.000 description 3
239000002245 particle Substances 0.000 description 3
229920000151 polyglycol Polymers 0.000 description 3
239000010695 polyglycol Substances 0.000 description 3
CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
208000000668 Chronic Pancreatitis Diseases 0.000 description 2
IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 2
DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
208000035467 Pancreatic insufficiency Diseases 0.000 description 2
206010033649 Pancreatitis chronic Diseases 0.000 description 2
238000002835 absorbance Methods 0.000 description 2
125000000129 anionic group Chemical group 0.000 description 2
239000003945 anionic surfactant Substances 0.000 description 2
230000008033 biological extinction Effects 0.000 description 2
238000005352 clarification Methods 0.000 description 2
230000003247 decreasing effect Effects 0.000 description 2
238000003745 diagnosis Methods 0.000 description 2
150000002170 ethers Chemical class 0.000 description 2
239000002563 ionic surfactant Substances 0.000 description 2
238000002156 mixing Methods 0.000 description 2
238000005375 photometry Methods 0.000 description 2
229960002101 secretin Drugs 0.000 description 2
239000011780 sodium chloride Substances 0.000 description 2
VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 2
238000012360 testing method Methods 0.000 description 2
YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 2
RMYMOVDNWWDGFU-DEYYWGMASA-N (2s)-2-[[(2s)-2-[[(2s)-2-aminopropanoyl]amino]propanoyl]amino]-n-(4-nitrophenyl)-3-phenylpropanamide Chemical compound C([C@H](NC(=O)[C@H](C)NC(=O)[C@@H](N)C)C(=O)NC=1C=CC(=CC=1)[N+]([O-])=O)C1=CC=CC=C1 RMYMOVDNWWDGFU-DEYYWGMASA-N 0.000 description 1
LQZZUXJYWNFBMV-UHFFFAOYSA-N 1-dodecanol group Chemical class C(CCCCCCCCCCC)O LQZZUXJYWNFBMV-UHFFFAOYSA-N 0.000 description 1
GKQHIYSTBXDYNQ-UHFFFAOYSA-M 1-dodecylpyridin-1-ium;chloride Chemical compound [Cl-].CCCCCCCCCCCC[N+]1=CC=CC=C1 GKQHIYSTBXDYNQ-UHFFFAOYSA-M 0.000 description 1
AQINKRZEWGMGCW-UHFFFAOYSA-L 1-dodecylpyridin-1-ium;sulfonato sulfate Chemical compound [O-]S(=O)(=O)OS([O-])(=O)=O.CCCCCCCCCCCC[N+]1=CC=CC=C1.CCCCCCCCCCCC[N+]1=CC=CC=C1 AQINKRZEWGMGCW-UHFFFAOYSA-L 0.000 description 1
JBVOQKNLGSOPNZ-UHFFFAOYSA-N 2-propan-2-ylbenzenesulfonic acid Chemical compound CC(C)C1=CC=CC=C1S(O)(=O)=O JBVOQKNLGSOPNZ-UHFFFAOYSA-N 0.000 description 1
QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
239000004382 Amylase Substances 0.000 description 1
102000013142 Amylases Human genes 0.000 description 1
108010065511 Amylases Proteins 0.000 description 1
KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 1
BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 1
241000283690 Bos taurus Species 0.000 description 1
OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
101800001982 Cholecystokinin Proteins 0.000 description 1
239000004471 Glycine Substances 0.000 description 1
240000004101 Iris pallida Species 0.000 description 1
102000004882 Lipase Human genes 0.000 description 1
108090001060 Lipase Proteins 0.000 description 1
239000004367 Lipase Substances 0.000 description 1
KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 description 1
208000034530 PLAA-associated neurodevelopmental disease Diseases 0.000 description 1
206010033645 Pancreatitis Diseases 0.000 description 1
206010035148 Plague Diseases 0.000 description 1
GOOHAUXETOMSMM-UHFFFAOYSA-N Propylene oxide Chemical compound CC1CO1 GOOHAUXETOMSMM-UHFFFAOYSA-N 0.000 description 1
JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical class C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 1
102100037505 Secretin Human genes 0.000 description 1
108010086019 Secretin Proteins 0.000 description 1
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 1
241000607479 Yersinia pestis Species 0.000 description 1
238000010521 absorption reaction Methods 0.000 description 1
OOVPFYDKQBSPHP-IUQUCOCYSA-N acetic acid;benzyl n-[(2s)-1-[[2-[[(2s)-5-(diaminomethylideneamino)-1-(4-nitroanilino)-1-oxopentan-2-yl]amino]-2-oxoethyl]amino]-3-methyl-1-oxobutan-2-yl]carbamate Chemical compound CC(O)=O.N([C@@H](C(C)C)C(=O)NCC(=O)N[C@@H](CCCN=C(N)N)C(=O)NC=1C=CC(=CC=1)[N+]([O-])=O)C(=O)OCC1=CC=CC=C1 OOVPFYDKQBSPHP-IUQUCOCYSA-N 0.000 description 1
230000003213 activating effect Effects 0.000 description 1
230000004913 activation Effects 0.000 description 1
150000008055 alkyl aryl sulfonates Chemical class 0.000 description 1
150000005215 alkyl ethers Chemical class 0.000 description 1
150000008051 alkyl sulfates Chemical class 0.000 description 1
235000019418 amylase Nutrition 0.000 description 1
229940027983 antiseptic and disinfectant quaternary ammonium compound Drugs 0.000 description 1
125000001797 benzyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])* 0.000 description 1
CADWTSSKOVRVJC-UHFFFAOYSA-N benzyl(dimethyl)azanium;chloride Chemical class [Cl-].C[NH+](C)CC1=CC=CC=C1 CADWTSSKOVRVJC-UHFFFAOYSA-N 0.000 description 1
229960003237 betaine Drugs 0.000 description 1
239000011575 calcium Substances 0.000 description 1
229910052791 calcium Inorganic materials 0.000 description 1
YMKDRGPMQRFJGP-UHFFFAOYSA-M cetylpyridinium chloride Chemical compound [Cl-].CCCCCCCCCCCCCCCC[N+]1=CC=CC=C1 YMKDRGPMQRFJGP-UHFFFAOYSA-M 0.000 description 1
235000019416 cholic acid Nutrition 0.000 description 1
239000002812 cholic acid derivative Chemical class 0.000 description 1
150000001842 cholic acids Chemical class 0.000 description 1
230000001684 chronic effect Effects 0.000 description 1
229940071118 cumenesulfonate Drugs 0.000 description 1
230000001419 dependent effect Effects 0.000 description 1
YRIUSKIDOIARQF-UHFFFAOYSA-N dodecyl benzenesulfonate Chemical compound CCCCCCCCCCCCOS(=O)(=O)C1=CC=CC=C1 YRIUSKIDOIARQF-UHFFFAOYSA-N 0.000 description 1
125000003438 dodecyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
229940071161 dodecylbenzenesulfonate Drugs 0.000 description 1
150000002191 fatty alcohols Chemical class 0.000 description 1
SYTBZMRGLBWNTM-UHFFFAOYSA-N flurbiprofen Chemical compound FC1=CC(C(C(O)=O)C)=CC=C1C1=CC=CC=C1 SYTBZMRGLBWNTM-UHFFFAOYSA-N 0.000 description 1
150000002314 glycerols Chemical class 0.000 description 1
238000011905 homologation Methods 0.000 description 1
239000000787 lecithin Substances 0.000 description 1
235000010445 lecithin Nutrition 0.000 description 1
235000019421 lipase Nutrition 0.000 description 1
125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
GSGDTSDELPUTKU-UHFFFAOYSA-N nonoxybenzene Chemical compound CCCCCCCCCOC1=CC=CC=C1 GSGDTSDELPUTKU-UHFFFAOYSA-N 0.000 description 1
239000003960 organic solvent Substances 0.000 description 1
125000000913 palmityl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
210000000496 pancreas Anatomy 0.000 description 1
230000004203 pancreatic function Effects 0.000 description 1
235000011837 pasties Nutrition 0.000 description 1
HCTVWSOKIJULET-LQDWTQKMSA-M phenoxymethylpenicillin potassium Chemical compound [K+].N([C@H]1[C@H]2SC([C@@H](N2C1=O)C([O-])=O)(C)C)C(=O)COC1=CC=CC=C1 HCTVWSOKIJULET-LQDWTQKMSA-M 0.000 description 1
229920000570 polyether Polymers 0.000 description 1
229920000642 polymer Polymers 0.000 description 1
239000000244 polyoxyethylene sorbitan monooleate Substances 0.000 description 1
235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
229920000053 polysorbate 80 Polymers 0.000 description 1
238000012545 processing Methods 0.000 description 1
QQONPFPTGQHPMA-UHFFFAOYSA-N propylene Natural products CC=C QQONPFPTGQHPMA-UHFFFAOYSA-N 0.000 description 1
125000004805 propylene group Chemical group [H]C([H])([H])C([H])([*:1])C([H])([H])[*:2] 0.000 description 1
150000003856 quaternary ammonium compounds Chemical group 0.000 description 1
230000035484 reaction time Effects 0.000 description 1
239000012898 sample dilution Substances 0.000 description 1
238000005070 sampling Methods 0.000 description 1
238000012216 screening Methods 0.000 description 1
OWMZNFCDEHGFEP-NFBCVYDUSA-N secretin human Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(N)=O)[C@@H](C)O)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC=1NC=NC=1)[C@@H](C)O)C1=CC=CC=C1 OWMZNFCDEHGFEP-NFBCVYDUSA-N 0.000 description 1
230000028327 secretion Effects 0.000 description 1
239000013049 sediment Substances 0.000 description 1
238000011896 sensitive detection Methods 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
IZTQOLKUZKXIRV-YRVFCXMDSA-N sincalide Chemical compound C([C@@H](C(=O)N[C@@H](CCSC)C(=O)NCC(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC=1C=CC=CC=1)C(N)=O)NC(=O)[C@@H](N)CC(O)=O)C1=CC=C(OS(O)(=O)=O)C=C1 IZTQOLKUZKXIRV-YRVFCXMDSA-N 0.000 description 1
235000019333 sodium laurylsulphate Nutrition 0.000 description 1
235000010344 sodium nitrate Nutrition 0.000 description 1
239000004317 sodium nitrate Substances 0.000 description 1
238000005063 solubilization Methods 0.000 description 1
230000007928 solubilization Effects 0.000 description 1
239000002904 solvent Substances 0.000 description 1
239000001590 sorbitan monolaureate Substances 0.000 description 1
235000011067 sorbitan monolaureate Nutrition 0.000 description 1
230000000638 stimulation Effects 0.000 description 1
239000000126 substance Substances 0.000 description 1
150000005846 sugar alcohols Polymers 0.000 description 1
239000000375 suspending agent Substances 0.000 description 1
230000002123 temporal effect Effects 0.000 description 1
229960004441 tyrosine Drugs 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
- C12Q2337/10—Anilides
- C12Q2337/12—Para-Nitroanilides p-NA
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/976—Trypsin; Chymotrypsin

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Microbiology (AREA)
Biochemistry (AREA)
Physics & Mathematics (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
Biophysics (AREA)
Analytical Chemistry (AREA)
Immunology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Investigating Or Analysing Biological Materials (AREA)

DD82243765A 1981-10-06 1982-10-04 Verfahren zur bestimmung der aktivitaet von chymotrypsin oder trypsin im stuhl DD203777A5 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
DE19813139719 DE3139719A1 (de)	1981-10-06	1981-10-06	Verfahren zur bestimmung der aktivitaet von chymotrypsin oder trypsin im stuhl

Publications (1)

Publication Number	Publication Date
DD203777A5 true DD203777A5 (de)	1983-11-02

Family

ID=6143516

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DD82243765A DD203777A5 (de)	1981-10-06	1982-10-04	Verfahren zur bestimmung der aktivitaet von chymotrypsin oder trypsin im stuhl

Country Status (12)

Country	Link
EP (1)	EP0076506B1 (da)
JP (1)	JPS6036758B2 (da)
AT (1)	ATE12521T1 (da)
AU (1)	AU534001B2 (da)
CA (1)	CA1191772A (da)
DD (1)	DD203777A5 (da)
DE (2)	DE3139719A1 (da)
DK (1)	DK156225C (da)
ES (1)	ES8306389A1 (da)
HU (1)	HU187416B (da)
IE (1)	IE53472B1 (da)
SU (1)	SU1367866A3 (da)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP6601148B2 (ja)	2015-10-27	2019-11-06	スズキ株式会社	エンジンの潤滑構造及び自動二輪車
JP7355140B2 (ja) *	2022-02-28	2023-10-03	住友ベークライト株式会社	セリンプロテアーゼの検出用または測定用試薬

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
IL42124A (en) *	1972-05-02	1977-02-28	Kabi Ab	Substrate for the determination of proteolytic enzymes
DE2829531A1 (de) *	1978-07-05	1980-01-24	Heuck Claus Christian Dr Rer N	Verfahren zur quantitativen bestimmung eines serumproteins in getruebten serum- und plasma-proben

1981
- 1981-10-06 DE DE19813139719 patent/DE3139719A1/de not_active Withdrawn
1982
- 1982-09-01 CA CA000410589A patent/CA1191772A/en not_active Expired
- 1982-09-02 IE IE2146/82A patent/IE53472B1/en not_active IP Right Cessation
- 1982-09-13 AU AU88350/82A patent/AU534001B2/en not_active Ceased
- 1982-09-20 SU SU823493078A patent/SU1367866A3/ru active
- 1982-09-30 ES ES516124A patent/ES8306389A1/es not_active Expired
- 1982-09-30 DK DK434082A patent/DK156225C/da not_active IP Right Cessation
- 1982-10-04 EP EP82109156A patent/EP0076506B1/de not_active Expired
- 1982-10-04 DD DD82243765A patent/DD203777A5/de not_active IP Right Cessation
- 1982-10-04 JP JP57174430A patent/JPS6036758B2/ja not_active Expired
- 1982-10-04 DE DE8282109156T patent/DE3262892D1/de not_active Expired
- 1982-10-04 AT AT82109156T patent/ATE12521T1/de not_active IP Right Cessation
- 1982-10-05 HU HU823190A patent/HU187416B/hu not_active IP Right Cessation

Also Published As

Publication number	Publication date
CA1191772A (en)	1985-08-13
EP0076506A1 (de)	1983-04-13
AU8835082A (en)	1983-05-05
AU534001B2 (en)	1983-12-22
JPS58111699A (ja)	1983-07-02
HU187416B (en)	1986-01-28
DK156225B (da)	1989-07-10
DK156225C (da)	1989-12-04
DE3262892D1 (en)	1985-05-09
ATE12521T1 (de)	1985-04-15
SU1367866A3 (ru)	1988-01-15
DK434082A (da)	1983-04-07
ES516124A0 (es)	1983-06-01
ES8306389A1 (es)	1983-06-01
IE53472B1 (en)	1988-11-23
IE822146L (en)	1983-04-06
JPS6036758B2 (ja)	1985-08-22
EP0076506B1 (de)	1985-04-03
DE3139719A1 (de)	1983-04-21

Legal Events

Date	Code	Title	Description
1997-05-22	ENJ	Ceased due to non-payment of renewal fee

Publication	Publication Date	Title
DE3502200C2 (da)	1991-02-21
DE69519160T2 (de)	2001-05-17	Verfahren zur quantitativen analyse von cholesterin
EP0250991B1 (de)	1993-01-27	Verfahren zur spezifischen Bestimmung des Serumfructosamingehalts sowie hierfür geeignetes Reagenzgemisch
EP0137269B1 (de)	1987-03-18	Verfahren zur gleichzeitigen Bestimmung von Fibrinogen und Fibrinogen-Spaltprodukten im Plasma
DE3636851A1 (de)	1988-05-11	Verfahren und reagenz zur spezifischen bestimmung des cholesterins der hdl-fraktion
DE3208253A1 (de)	1983-09-15	Verfahren zur spezifischen bestimmung des cholesterins der ldl-fraktion im serum
EP0083773A1 (de)	1983-07-20	Verfahren zur Herstellung eines Gewebsthromboplastinpräparates
EP0012184A1 (de)	1980-06-25	Hämolysierlösung, Verwendung einer solchen für die Bestimmung von Glucose im Blut, und Hämolyseverfahren
DE68912115T2 (de)	1994-08-11	Ausscheidungsverfahren für die herstellung von thromboplastinreagenzien.
EP0062310B1 (de)	1985-12-11	Reagens zur optischen Bestimmung des Blutgerinnungsverhaltens
EP0185335B1 (de)	1991-04-24	Verfahren zum Nachweis des Vorliegens einer Allergie und zum spezifischen Nachweis des für die Allergie verantwortlichen Allergens
DE69428597T2 (de)	2002-10-10	Reinigungslösung für analysegerät
DE2654189C3 (de)	1985-10-03	Verfahren zur in-vitro-Bestimmung der biologischen Aktivität von Faktor Xa-Inhibitor im Blut
EP0468481A1 (de)	1992-01-29	Nichtionische Blockcopolymere aus Propylenoxid und Ethylenoxid
DE69013834T2 (de)	1995-03-23	Reagenz zum Nachweis und zur quantitativen Bestimmung von Leukozyten in biologischen Flüssigkeiten und Verfahren zu seiner Verwendung.
DE1940816A1 (de)	1971-06-09	Verfahren und diagnostisches Mittel zur enzymatischen Bestimmung von Glucose
DD203777A5 (de)	1983-11-02	Verfahren zur bestimmung der aktivitaet von chymotrypsin oder trypsin im stuhl
DE2846408A1 (de)	1979-05-10	Verfahren zur photometrischen bestimmung der anwesenheit von protein in einem unbekannten material
EP1040354B1 (de)	2004-03-17	Bestimmung von in lipoprotein enthaltenem triglycerid
DE112013002316T5 (de)	2015-04-23	Systeme und Verfahren für Assays von im nicht-nüchternen Zustand gewonnenem LDL-Cholesterin
AT399055B (de)	1995-03-27	Verfahren zur bestimmung von fibrinogen
EP0461473B1 (de)	1996-03-27	Verfahren und Mittel zur Beseitigung von Trübungen in biologischen Flüssigkeiten
EP0408075B1 (de)	1995-03-08	Verfahren und Reagenz zur Bestimmung von Antithrombin III
DE69615991T2 (de)	2002-04-11	Verfahren und Reagenz zum Nachweis von Ionen unter Verwendung von Maltose Derivaten
DE2059278A1 (de)	1971-07-22	Diagnostische Testpackung und diagnostisches Testverfahren zur Bestimmung der alkalischen Phosphatase