DD224052A5 - Verfahren und mittel zur empfindlichkeitspruefung von bakterien - Google Patents

Verfahren und mittel zur empfindlichkeitspruefung von bakterien Download PDF

Info

Publication number: DD224052A5
Authority: DD; German Democratic Republic
Prior art keywords: bacteria; test; antibiotic; enzyme; cytoplasmic
Prior art date: 1983-08-02

Application number

DD84265887A

Other languages

German (de)

English (en)

Inventor

Manfred Kappner

Harald Metz

Original Assignee

Merck Patent Gmbh

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1983-08-02

Filing date

1984-08-01

Publication date

1985-06-26

1984-08-01 Application filed by Merck Patent Gmbh filed Critical Merck Patent Gmbh

1985-06-26 Publication of DD224052A5 publication Critical patent/DD224052A5/de

Links

238000012360 testing method Methods 0.000 title claims abstract description 43
241000894006 Bacteria Species 0.000 title claims abstract description 35
238000000034 method Methods 0.000 title claims abstract description 15
230000035945 sensitivity Effects 0.000 title description 14
102000004190 Enzymes Human genes 0.000 claims abstract description 31
108090000790 Enzymes Proteins 0.000 claims abstract description 31
230000003115 biocidal effect Effects 0.000 claims abstract description 21
230000000694 effects Effects 0.000 claims abstract description 21
239000003242 anti bacterial agent Substances 0.000 claims abstract description 16
229940088710 antibiotic agent Drugs 0.000 claims abstract description 15
230000001086 cytosolic effect Effects 0.000 claims abstract description 15
239000000758 substrate Substances 0.000 claims abstract description 12
239000000203 mixture Substances 0.000 claims description 10
239000005515 coenzyme Substances 0.000 claims description 6
239000003599 detergent Substances 0.000 claims description 6
235000015097 nutrients Nutrition 0.000 claims description 6
239000002824 redox indicator Substances 0.000 claims description 6
102000004316 Oxidoreductases Human genes 0.000 claims description 3
108090000854 Oxidoreductases Proteins 0.000 claims description 3
230000003301 hydrolyzing effect Effects 0.000 claims 1
230000015572 biosynthetic process Effects 0.000 abstract description 9
241001529936 Murinae Species 0.000 abstract description 5
230000009290 primary effect Effects 0.000 abstract description 2
230000001580 bacterial effect Effects 0.000 description 14
AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 11
229960000723 ampicillin Drugs 0.000 description 11
239000003782 beta lactam antibiotic agent Substances 0.000 description 8
238000011534 incubation Methods 0.000 description 8
239000008363 phosphate buffer Substances 0.000 description 8
DYDCUQKUCUHJBH-UWTATZPHSA-N D-Cycloserine Chemical compound N[C@@H]1CONC1=O DYDCUQKUCUHJBH-UWTATZPHSA-N 0.000 description 7
DYDCUQKUCUHJBH-UHFFFAOYSA-N D-Cycloserine Natural products NC1CONC1=O DYDCUQKUCUHJBH-UHFFFAOYSA-N 0.000 description 7
238000003556 assay Methods 0.000 description 7
229960003077 cycloserine Drugs 0.000 description 7
239000000725 suspension Substances 0.000 description 6
229920001817 Agar Polymers 0.000 description 5
108010013639 Peptidoglycan Proteins 0.000 description 5
239000008272 agar Substances 0.000 description 5
238000004458 analytical method Methods 0.000 description 5
230000002401 inhibitory effect Effects 0.000 description 5
239000002609 medium Substances 0.000 description 5
244000005700 microbiome Species 0.000 description 5
239000012085 test solution Substances 0.000 description 5
241000588724 Escherichia coli Species 0.000 description 4
239000000872 buffer Substances 0.000 description 4
-1 for example Natural products 0.000 description 4
229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 4
241000894007 species Species 0.000 description 4
229930186147 Cephalosporin Natural products 0.000 description 3
102000013460 Malate Dehydrogenase Human genes 0.000 description 3
108010026217 Malate Dehydrogenase Proteins 0.000 description 3
229930182555 Penicillin Natural products 0.000 description 3
239000002202 Polyethylene glycol Substances 0.000 description 3
239000006161 blood agar Substances 0.000 description 3
229940124587 cephalosporin Drugs 0.000 description 3
150000001780 cephalosporins Chemical class 0.000 description 3
239000012895 dilution Substances 0.000 description 3
238000010790 dilution Methods 0.000 description 3
230000005764 inhibitory process Effects 0.000 description 3
BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 3
150000002960 penicillins Chemical class 0.000 description 3
229920001223 polyethylene glycol Polymers 0.000 description 3
JNYAEWCLZODPBN-JGWLITMVSA-N (2r,3r,4s)-2-[(1r)-1,2-dihydroxyethyl]oxolane-3,4-diol Chemical class OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O JNYAEWCLZODPBN-JGWLITMVSA-N 0.000 description 2
108010001478 Bacitracin Proteins 0.000 description 2
241000588919 Citrobacter freundii Species 0.000 description 2
241000588697 Enterobacter cloacae Species 0.000 description 2
241000192125 Firmicutes Species 0.000 description 2
102000005744 Glycoside Hydrolases Human genes 0.000 description 2
108010031186 Glycoside Hydrolases Proteins 0.000 description 2
238000002768 Kirby-Bauer method Methods 0.000 description 2
JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
108010059993 Vancomycin Proteins 0.000 description 2
XJLXINKUBYWONI-DQQFMEOOSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-DQQFMEOOSA-N 0.000 description 2
238000013459 approach Methods 0.000 description 2
229960003071 bacitracin Drugs 0.000 description 2
229930184125 bacitracin Natural products 0.000 description 2
CLKOFPXJLQSYAH-ABRJDSQDSA-N bacitracin A Chemical compound C1SC([C@@H](N)[C@@H](C)CC)=N[C@@H]1C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]1C(=O)N[C@H](CCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@H](CC=2C=CC=CC=2)C(=O)N[C@@H](CC=2N=CNC=2)C(=O)N[C@H](CC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)NCCCC1 CLKOFPXJLQSYAH-ABRJDSQDSA-N 0.000 description 2
244000052616 bacterial pathogen Species 0.000 description 2
239000003593 chromogenic compound Substances 0.000 description 2
MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical compound OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 description 2
239000007850 fluorescent dye Substances 0.000 description 2
230000012010 growth Effects 0.000 description 2
244000052769 pathogen Species 0.000 description 2
108010009004 proteose-peptone Proteins 0.000 description 2
238000010561 standard procedure Methods 0.000 description 2
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
229960003165 vancomycin Drugs 0.000 description 2
MYPYJXKWCTUITO-UHFFFAOYSA-N vancomycin Natural products O1C(C(=C2)Cl)=CC=C2C(O)C(C(NC(C2=CC(O)=CC(O)=C2C=2C(O)=CC=C3C=2)C(O)=O)=O)NC(=O)C3NC(=O)C2NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(CC(C)C)NC)C(O)C(C=C3Cl)=CC=C3OC3=CC2=CC1=C3OC1OC(CO)C(O)C(O)C1OC1CC(C)(N)C(O)C(C)O1 MYPYJXKWCTUITO-UHFFFAOYSA-N 0.000 description 2
MYPYJXKWCTUITO-LYRMYLQWSA-O vancomycin(1+) Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=C2C=C3C=C1OC1=CC=C(C=C1Cl)[C@@H](O)[C@H](C(N[C@@H](CC(N)=O)C(=O)N[C@H]3C(=O)N[C@H]1C(=O)N[C@H](C(N[C@@H](C3=CC(O)=CC(O)=C3C=3C(O)=CC=C1C=3)C([O-])=O)=O)[C@H](O)C1=CC=C(C(=C1)Cl)O2)=O)NC(=O)[C@@H](CC(C)C)[NH2+]C)[C@H]1C[C@](C)([NH3+])[C@H](O)[C@H](C)O1 MYPYJXKWCTUITO-LYRMYLQWSA-O 0.000 description 2
WQZGKKKJIJFFOK-SVZMEOIVSA-N (+)-Galactose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-SVZMEOIVSA-N 0.000 description 1
VXQPHDBRWYMWKH-UHFFFAOYSA-M 1,2-diphenyltetrazol-1-ium;bromide Chemical compound [Br-].C=1C=CC=CC=1[N+]1=CN=NN1C1=CC=CC=C1 VXQPHDBRWYMWKH-UHFFFAOYSA-M 0.000 description 1
JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
125000006306 4-iodophenyl group Chemical group [H]C1=C([H])C(*)=C([H])C([H])=C1I 0.000 description 1
VDJKJPMLWJWQIH-UHFFFAOYSA-M 5-ethylphenazin-5-ium;ethyl sulfate Chemical compound CCOS([O-])(=O)=O.C1=CC=C2[N+](CC)=C(C=CC=C3)C3=NC2=C1 VDJKJPMLWJWQIH-UHFFFAOYSA-M 0.000 description 1
RXGJTUSBYWCRBK-UHFFFAOYSA-M 5-methylphenazinium methyl sulfate Chemical compound COS([O-])(=O)=O.C1=CC=C2[N+](C)=C(C=CC=C3)C3=NC2=C1 RXGJTUSBYWCRBK-UHFFFAOYSA-M 0.000 description 1
HOKIDJSKDBPKTQ-GLXFQSAKSA-N Cephalosporin C Natural products S1CC(COC(=O)C)=C(C(O)=O)N2C(=O)[C@@H](NC(=O)CCC[C@@H](N)C(O)=O)[C@@H]12 HOKIDJSKDBPKTQ-GLXFQSAKSA-N 0.000 description 1
241000588923 Citrobacter Species 0.000 description 1
108090000204 Dipeptidase 1 Proteins 0.000 description 1
241000588914 Enterobacter Species 0.000 description 1
241000588921 Enterobacteriaceae Species 0.000 description 1
241000588722 Escherichia Species 0.000 description 1
239000007995 HEPES buffer Substances 0.000 description 1
241000588731 Hafnia Species 0.000 description 1
102000004157 Hydrolases Human genes 0.000 description 1
108090000604 Hydrolases Proteins 0.000 description 1
241000588748 Klebsiella Species 0.000 description 1
239000006154 MacConkey agar Substances 0.000 description 1
229930195708 Penicillin V Natural products 0.000 description 1
239000001888 Peptone Substances 0.000 description 1
108010080698 Peptones Proteins 0.000 description 1
241000607720 Serratia Species 0.000 description 1
IYFATESGLOUGBX-YVNJGZBMSA-N Sorbitan monopalmitate Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O IYFATESGLOUGBX-YVNJGZBMSA-N 0.000 description 1
235000019764 Soybean Meal Nutrition 0.000 description 1
102000019259 Succinate Dehydrogenase Human genes 0.000 description 1
108010012901 Succinate Dehydrogenase Proteins 0.000 description 1
239000007983 Tris buffer Substances 0.000 description 1
238000002835 absorbance Methods 0.000 description 1
238000011481 absorbance measurement Methods 0.000 description 1
230000003698 anagen phase Effects 0.000 description 1
125000000129 anionic group Chemical group 0.000 description 1
230000000844 anti-bacterial effect Effects 0.000 description 1
239000002246 antineoplastic agent Substances 0.000 description 1
102000005936 beta-Galactosidase Human genes 0.000 description 1
108010005774 beta-Galactosidase Proteins 0.000 description 1
102000006635 beta-lactamase Human genes 0.000 description 1
230000008033 biological extinction Effects 0.000 description 1
210000004556 brain Anatomy 0.000 description 1
230000009172 bursting Effects 0.000 description 1
125000002091 cationic group Chemical group 0.000 description 1
CZTQZXZIADLWOZ-CRAIPNDOSA-N cefaloridine Chemical compound O=C([C@@H](NC(=O)CC=1SC=CC=1)[C@H]1SC2)N1C(C(=O)[O-])=C2C[N+]1=CC=CC=C1 CZTQZXZIADLWOZ-CRAIPNDOSA-N 0.000 description 1
229960003866 cefaloridine Drugs 0.000 description 1
HOKIDJSKDBPKTQ-GLXFQSAKSA-M cephalosporin C(1-) Chemical compound S1CC(COC(=O)C)=C(C([O-])=O)N2C(=O)[C@@H](NC(=O)CCC[C@@H]([NH3+])C([O-])=O)[C@@H]12 HOKIDJSKDBPKTQ-GLXFQSAKSA-M 0.000 description 1
239000003153 chemical reaction reagent Substances 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
229940127089 cytotoxic agent Drugs 0.000 description 1
230000003111 delayed effect Effects 0.000 description 1
238000002848 electrochemical method Methods 0.000 description 1
239000012992 electron transfer agent Substances 0.000 description 1
238000011156 evaluation Methods 0.000 description 1
230000009036 growth inhibition Effects 0.000 description 1
CJNBYAVZURUTKZ-UHFFFAOYSA-N hafnium(IV) oxide Inorganic materials O=[Hf]=O CJNBYAVZURUTKZ-UHFFFAOYSA-N 0.000 description 1
238000002847 impedance measurement Methods 0.000 description 1
238000000338 in vitro Methods 0.000 description 1
239000002054 inoculum Substances 0.000 description 1
230000003834 intracellular effect Effects 0.000 description 1
238000005259 measurement Methods 0.000 description 1
230000003204 osmotic effect Effects 0.000 description 1
KHPXUQMNIQBQEV-UHFFFAOYSA-N oxaloacetic acid Chemical compound OC(=O)CC(=O)C(O)=O KHPXUQMNIQBQEV-UHFFFAOYSA-N 0.000 description 1
230000001717 pathogenic effect Effects 0.000 description 1
230000035515 penetration Effects 0.000 description 1
229940056360 penicillin g Drugs 0.000 description 1
229940056367 penicillin v Drugs 0.000 description 1
235000019319 peptone Nutrition 0.000 description 1
BPLBGHOLXOTWMN-MBNYWOFBSA-N phenoxymethylpenicillin Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)COC1=CC=CC=C1 BPLBGHOLXOTWMN-MBNYWOFBSA-N 0.000 description 1
230000004962 physiological condition Effects 0.000 description 1
238000006479 redox reaction Methods 0.000 description 1
239000007787 solid Substances 0.000 description 1
239000000243 solution Substances 0.000 description 1
239000001570 sorbitan monopalmitate Substances 0.000 description 1
229940031953 sorbitan monopalmitate Drugs 0.000 description 1
235000011071 sorbitan monopalmitate Nutrition 0.000 description 1
239000004455 soybean meal Substances 0.000 description 1
238000010998 test method Methods 0.000 description 1
125000003831 tetrazolyl group Chemical group 0.000 description 1
238000002560 therapeutic procedure Methods 0.000 description 1
238000012546 transfer Methods 0.000 description 1
230000000007 visual effect Effects 0.000 description 1
YTEJSAFVYHDCSN-UHFFFAOYSA-K zinc;benzo[a]phenoxazin-9-ylidene(dimethyl)azanium;trichloride Chemical compound [Cl-].[Cl-].[Cl-].[Zn+2].C1=CC=C2C(N=C3C=CC(C=C3O3)=[N+](C)C)=C3C=CC2=C1 YTEJSAFVYHDCSN-UHFFFAOYSA-K 0.000 description 1
239000002132 β-lactam antibiotic Substances 0.000 description 1
229940124586 β-lactam antibiotics Drugs 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
- C12Q2334/20—Coumarin derivatives
- C12Q2334/22—4-Methylumbelliferyl, i.e. beta-methylumbelliferone, 4MU
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Analytical Chemistry (AREA)
Toxicology (AREA)
Immunology (AREA)
Microbiology (AREA)
Molecular Biology (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Biotechnology (AREA)
Biochemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

DD84265887A 1983-08-02 1984-08-01 Verfahren und mittel zur empfindlichkeitspruefung von bakterien DD224052A5 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
DE19833327839 DE3327839A1 (de)	1983-08-02	1983-08-02	Verfahren und mittel zur empfindlichkeitspruefung von bakterien

Publications (1)

Publication Number	Publication Date
DD224052A5 true DD224052A5 (de)	1985-06-26

Family

ID=6205555

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DD84265887A DD224052A5 (de)	1983-08-02	1984-08-01	Verfahren und mittel zur empfindlichkeitspruefung von bakterien

Country Status (7)

Country	Link
US (1)	US4622297A (fr)
EP (1)	EP0135023B1 (fr)
JP (1)	JPS6054698A (fr)
DD (1)	DD224052A5 (fr)
DE (2)	DE3327839A1 (fr)
IL (1)	IL72535A (fr)
ZA (1)	ZA845966B (fr)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE3429823A1 (de) *	1984-08-14	1986-02-27	Merck Patent Gmbh, 6100 Darmstadt	Mittel und verfahren zum nachweis antimikrobiell wirksamer substanzen
US4774173A (en) *	1984-12-06	1988-09-27	Orgenics Ltd.	Microbiological assay kit and method for detecting de novo biomolecular synthesis inhibitors
US4704357A (en) *	1985-09-30	1987-11-03	United States Of America As Represented By The Department Of Health And Human Services	Immortalized T-lymphocyte cell line for testing HTLV-III inactivation
US4925789A (en) *	1986-06-30	1990-05-15	Edberg Stephen C	Method and medium for use in detecting target microbes in situ in a specimen sample of a possibly contaminated material
US5429933A (en)	1986-06-30	1995-07-04	Edberg; Stephen C.	Detection of first generation environmental sourced microbes in an environmentally-derived sample
IL85948A0 (en) *	1987-04-08	1988-09-30	Cambridge Bioscience Corp	Method and kit for detecting bacteria or fungi
AU2584588A (en) *	1987-12-01	1989-06-01	Abbott Laboratories	Antibiotic resistance testing assay
IL91596A0 (en) *	1989-09-11	1990-04-29	Orgenics Ltd	Microbiological assay kit and method for detecting antibacterial compounds
US5510243A (en) *	1994-06-21	1996-04-23	Gelman Sciences, Inc.	Multiple chromogen enzyme targeting (MCET) for use in bacterial contamination monitoring
US5700655A (en) *	1995-11-14	1997-12-23	Idexx Laboratories, Inc.	Method for quantification of biological material in a sample
US5985594A (en) *	1995-11-14	1999-11-16	Idexx Laboratories, Inc.	Method for quantification of biological material in a sample
US7122338B2 (en) *	1995-11-14	2006-10-17	Biocontrol Systems, Inc.	Method for quantification of biological material in a sample
EP0822261B1 (fr) *	1996-07-29	2002-07-03	Academia Sinica	Méthode rapide pour la détection de la susceptibilité microbienne
US7067500B2 (en) *	1997-05-02	2006-06-27	Integrated Research Technology, Llc	Betaines as adjuvants to susceptibility testing and antimicrobial therapy
US6406880B1 (en)	1997-05-02	2002-06-18	Integrated Research Technology, Llc	Betaines as adjuvants to susceptibility testing and antimicrobial therapy
CA2288457A1 (fr) *	1997-05-02	1998-11-12	Integrated Research Technology, Llc	Utilisation de betaines comme adjuvants d'essais de sensibilite et d'une therapie anti-microbienne
US6548021B1 (en)	1997-10-10	2003-04-15	President And Fellows Of Harvard College	Surface-bound, double-stranded DNA protein arrays
US6720139B1 (en)	1999-01-27	2004-04-13	Elitra Pharmaceuticals, Inc.	Genes identified as required for proliferation in Escherichia coli
US6589738B1 (en)	1999-11-09	2003-07-08	Elitra Pharmaceuticals, Inc.	Genes essential for microbial proliferation and antisense thereto
US6750038B1 (en) *	2000-09-28	2004-06-15	Essential Therapeutics, Inc.	Rapid antibiotic susceptibility test
GB0025084D0 (en) *	2000-10-13	2000-11-29	Cambridge Meditech	Improvements in detection
US20040029129A1 (en) *	2001-10-25	2004-02-12	Liangsu Wang	Identification of essential genes in microorganisms
US20030170694A1 (en) *	2001-12-21	2003-09-11	Daniel Wall	Stabilized nucleic acids in gene and drug discovery and methods of use
ES2396820B1 (es)	2011-07-26	2014-01-31	Universidad Autónoma de Madrid	Método para evaluar la integridad de la pared celular bacteriana.
EP2821499B1 (fr)	2013-07-04	2017-02-15	ABM Technologies, LLC	Procédé pour la détermination rapide de la sensibilité ou de la résistance de bactéries aux antibiotiques
DE102013225037B4 (de)	2013-12-05	2016-08-25	Asklepios Kliniken Verwaltungsgesellschaft mbH	Verfahren zur Erkennung resistenter Keime und Vorrichtung zum Durchführen desselben
EP3048174B1 (fr)	2015-01-21	2018-11-07	ABM Technologies, LLC	Procédure pour la détermination rapide de la sensibilité de bactéries à des antibiotiques qui inhibent la synthèse protéique

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US3197384A (en) *	1960-12-13	1965-07-27	Harold C Herman	Process for the determination of microbial sensitivity to antimicrobial agents
US3506544A (en) *	1964-10-09	1970-04-14	Magna Corp	Method of determining microbial populations,enzyme activities,and substrate concentrations by electrochemical analysis
US3509026A (en) *	1967-01-19	1970-04-28	Litton Systems Inc	Method of and apparatus for indicating the sensitivity of microorganisms to antibiotics
US4242446A (en) *	1978-07-26	1980-12-30	Coulter Electronics, Inc.	Method for determining a substance in a biological fluid and reagent combination for use in the method
DE2930394A1 (de) *	1978-07-26	1980-02-14	Seppo Kolehmainen	Verfahren zur bestimmung des antibiotikaspiegels in einem medium
GB2026693B (en) *	1978-07-26	1983-02-09	Tarkkanen V	Determination of antibiotic levels in a medium
JPH0321160B2 (fr) *	1979-05-02	1991-03-22	Nat Res Dev
ATE12520T1 (de) *	1980-12-05	1985-04-15	Battelle Memorial Institute	Verfahren zum beobachten der entwicklung von mikroorganismen.
DE3380263D1 (en) *	1982-04-14	1989-08-31	Radiometer Corporate Dev Limit	Microbiological test processes and apparatus

1983
- 1983-08-02 DE DE19833327839 patent/DE3327839A1/de not_active Withdrawn
1984
- 1984-07-17 EP EP84108367A patent/EP0135023B1/fr not_active Expired
- 1984-07-17 DE DE8484108367T patent/DE3474160D1/de not_active Expired
- 1984-07-29 IL IL72535A patent/IL72535A/xx unknown
- 1984-08-01 ZA ZA845966A patent/ZA845966B/xx unknown
- 1984-08-01 DD DD84265887A patent/DD224052A5/de unknown
- 1984-08-02 US US06/637,192 patent/US4622297A/en not_active Expired - Fee Related
- 1984-08-02 JP JP59161664A patent/JPS6054698A/ja active Pending

Also Published As

Publication number	Publication date
US4622297A (en)	1986-11-11
DE3474160D1 (en)	1988-10-27
DE3327839A1 (de)	1985-02-14
EP0135023A2 (fr)	1985-03-27
EP0135023B1 (fr)	1988-09-21
ZA845966B (en)	1985-03-27
IL72535A (en)	1988-10-31
IL72535A0 (en)	1984-11-30
EP0135023A3 (en)	1986-03-26
JPS6054698A (ja)	1985-03-29

Publication	Publication Date	Title
DD224052A5 (de)	1985-06-26	Verfahren und mittel zur empfindlichkeitspruefung von bakterien
DE60215339T2 (de)	2007-05-03	Spezifische detektionsmedien für staphylococcus aureus und identifizierungs- und/oder zaehlmethode unter verwendung besagter detektionsmedien
DE69020555T2 (de)	1995-11-02	Fällungstest für mikroorganismen.
DE69122385T2 (de)	1997-04-10	Neues und verbessertes verfahren zur bestimmung von e. coli in wasser
DE69531007T2 (de)	2004-04-01	Medium zum nachweis von enterococci in einer probe
DE69421085T2 (de)	2000-01-27	Verfahren und testsatz zum nachweis von bakteriophagen
DE69219989T2 (de)	1997-11-27	Schneller auswahltest für induzierbare beta-laktamase
DE69737786T2 (de)	2008-02-07	Testmedium und quantitative verfahren für den nachweis und die unterscheidung von biologischen materialen in einer testprobe
DE69018567T2 (de)	1995-08-10	Verfahren zum Nachweis von Mikroorganismen durch Verwendung von Resorufin oder Orcirufin.
DE60310583T3 (de)	2012-09-27	Verfahren zum nachweis von methicillinresistenten staphylococcus aureus
US4014745A (en)	1977-03-29	Application of luciferase assay for ATP to antimicrobial drug susceptibility
EP0625581B1 (fr)	1998-12-30	Procédé pour la détection optique des micro-organismes, leur identification et test de sensibilité contre les antibiotiques utilisant une système à indicateur redox
DE69814361T2 (de)	2004-02-19	Identifikation von salmonella
DE69716644T2 (de)	2003-07-03	Verfahren und medium zum nachweis von vancomycin-resistenten enterococcus
US5411867A (en)	1995-05-02	Method for determination of E. coli in water
DE69836275T2 (de)	2007-05-31	Zellkulturmedien zum spezifischen nachweis von verschiedenen candida-spezies, sowie testmethoden
US8404460B2 (en)	2013-03-26	Method for detecting and/or identifying Clostridium difficile
US4340671A (en)	1982-07-20	E. coli sensitivity broth
DE4324392A1 (de)	1995-01-26	Kulturmedium für den gleichzeitigen Nachweis von coliformen Bakterien und Escherichia coli
DE3429823A1 (de)	1986-02-27	Mittel und verfahren zum nachweis antimikrobiell wirksamer substanzen
US6130057A (en)	2000-10-10	Method for differentiating microorganisms in a sample
Van Horn et al.	1998	Colonization and microbiology of the motile enterococci in a patient population
DE69219734T2 (de)	1997-09-11	Mikrobiologisches analytisches Milieu und Verfahren zum Nachweis von Hefen der Art Candida albicans
JP2001008679A (ja)	2001-01-16	大腸菌ｏ２６分離用培地
DE60025380T3 (de)	2010-02-11	Ein-schritttest zum nachweis von antimikrobiellen rückständen in eiern