DD270643A3 - Laserfluorometerdetektor für die Mikrosäulenchromatografie - Google Patents

Laserfluorometerdetektor für die Mikrosäulenchromatografie Download PDF

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Publication number
DD270643A3
DD270643A3 DD28988586A DD28988586A DD270643A3 DD 270643 A3 DD270643 A3 DD 270643A3 DD 28988586 A DD28988586 A DD 28988586A DD 28988586 A DD28988586 A DD 28988586A DD 270643 A3 DD270643 A3 DD 270643A3
Authority
DD
German Democratic Republic
Prior art keywords
laser
detector
column chromatography
micro column
laser fluorometer
Prior art date
Application number
DD28988586A
Other languages
English (en)
Inventor
Aleksandr F Lobazov
Vasilij A Mostovnikov
Sergej V Necaev
Boris G Belenkij
Original Assignee
Inst Fiz An Bssr
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Fiz An Bssr filed Critical Inst Fiz An Bssr
Publication of DD270643A3 publication Critical patent/DD270643A3/de

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6402—Atomic fluorescence; Laser induced fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/74—Optical detectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • G01N21/85—Investigating moving fluids or granular solids

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Optics & Photonics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Optical Measuring Cells (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Silicon Compounds (AREA)

Abstract

Die Erfindung betrifft die Laserspektroskopie und kann in Laserfluorometern fuer die Mikrosaeulenchromatografie ausgenutzt werden. Ziel der Erfindung ist die Erhoehung der Empfindlichkeit und des Aufloesungsvermoegens des Detektors. In dem Laserfluorometerdetektor ist das System, das die Laserstrahlung erzeugt, als zylindrisches Fernrohr 2 und trapezfoermige Blende 3 ausgefuehrt. Das zylindrische Fernrohr 2 wandelt die Laserstrahlung in der Projektion in einen rechteckigen Streifen um, der nach der Form dem Kanal mit der zu untersuchenden Fluessigkeit in der Kuevette 4 mit Abfluss entspricht. Die Blende 3 regelt die Laenge des rechteckigen Streifens entsprechend der Groesse des chromatografischen Zackens. Fig. 1

Description

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Claims (1)

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    ε t
DD28988586A 1985-05-12 1986-05-05 Laserfluorometerdetektor für die Mikrosäulenchromatografie DD270643A3 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SU853893760A SU1376042A1 (ru) 1985-05-12 1985-05-12 Лазерный флуориметрический детектор дл микроколоночной хроматографии

Publications (1)

Publication Number Publication Date
DD270643A3 true DD270643A3 (de) 1989-08-09

Family

ID=21176583

Family Applications (1)

Application Number Title Priority Date Filing Date
DD28988586A DD270643A3 (de) 1985-05-12 1986-05-05 Laserfluorometerdetektor für die Mikrosäulenchromatografie

Country Status (10)

Country Link
US (1) US4822167A (de)
AT (1) AT390841B (de)
CS (1) CS268787B1 (de)
DD (1) DD270643A3 (de)
DE (1) DE3627448A1 (de)
FR (1) FR2603106B1 (de)
GB (1) GB2193571B (de)
HU (1) HU201155B (de)
SE (1) SE458156B (de)
SU (1) SU1376042A1 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6830731B1 (en) * 1998-01-05 2004-12-14 Biosite, Inc. Immunoassay fluorometer
US6195214B1 (en) * 1999-07-30 2001-02-27 Etec Systems, Inc. Microcolumn assembly using laser spot welding

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4031399A (en) * 1975-02-24 1977-06-21 Beckman Instruments, Inc. Fluorometer
US3999861A (en) * 1975-06-30 1976-12-28 Technicon Instruments Corporation Flow cell
FR2348486A1 (fr) * 1976-04-15 1977-11-10 Commissariat Energie Atomique Procede et dispositif d'analyse d'echantillon par spectrographie d'emission utilisant un faisceau laser
US4203670A (en) * 1977-04-21 1980-05-20 Bromberg Nathan S System and method of fluorescence polarimetry
US4284355A (en) * 1979-10-29 1981-08-18 Ortho Diagnostics, Inc. Automated method for cell volume determination
US4350892A (en) * 1980-07-31 1982-09-21 Research Corporation X'-, Y'-, Z'- axis multidimensional slit-scan flow system
DD159566A1 (de) * 1981-06-10 1983-03-16 Hartmut Lucht Spektralfluorometer
DE3208919A1 (de) * 1982-03-12 1983-09-22 Gesellschaft für Strahlen- und Umweltforschung mbH, 8000 München Anordnung zur messung der fluoreszenzpolarisation
US4537861A (en) * 1983-02-03 1985-08-27 Elings Virgil B Apparatus and method for homogeneous immunoassay
US4555177A (en) * 1983-12-22 1985-11-26 The United States Of America As Represented By The Secretary Of The Navy Method and apparatus for detecting singlet state resonance fluorescence
US4643566A (en) * 1984-07-20 1987-02-17 Canon Kabushiki Kaisha Particle analyzing apparatus
SE455646B (sv) * 1984-10-22 1988-07-25 Radians Innova Ab Fluorescensanordning

Also Published As

Publication number Publication date
FR2603106B1 (fr) 1988-12-09
HUT44652A (en) 1988-03-28
CS268787B1 (en) 1990-04-11
SE458156B (sv) 1989-02-27
CS300886A1 (en) 1989-06-13
US4822167A (en) 1989-04-18
GB2193571A (en) 1988-02-10
HU201155B (en) 1990-09-28
FR2603106A1 (fr) 1988-02-26
GB2193571B (en) 1990-07-04
DE3627448C2 (de) 1990-02-22
SE8603622D0 (sv) 1986-08-27
SU1376042A1 (ru) 1988-02-23
AT390841B (de) 1990-07-10
SE8603622L (sv) 1988-02-28
GB8619282D0 (en) 1986-09-17
ATA209286A (de) 1989-12-15
DE3627448A1 (de) 1988-02-18

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