DD281659A5 - Verfahren zur konzentrationsbestimmung von cyclosporinen - Google Patents

Verfahren zur konzentrationsbestimmung von cyclosporinen Download PDF

Info

Publication number: DD281659A5
Authority: DD; German Democratic Republic
Prior art keywords: concentration; cyclosporins; cyclosporin; enzyme; inhibition
Prior art date: 1988-09-07

Application number

DD88319577A

Other languages

German (de)

English (en)

Inventor

Gunter Fischer

Gerhard Kuellertz

Original Assignee

Univ Halle Wittenberg

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1988-09-07

Filing date

1988-09-07

Publication date

1990-08-15

1988-09-07 Application filed by Univ Halle Wittenberg filed Critical Univ Halle Wittenberg

1988-09-07 Priority to DD88319577A priority Critical patent/DD281659A5/de

1989-08-26 Priority to EP89115759A priority patent/EP0360029B1/fr

1989-08-26 Priority to ES89115759T priority patent/ES2058425T3/es

1989-08-26 Priority to DE8989115759T priority patent/DE58905125D1/de

1989-08-26 Priority to AT89115759T priority patent/ATE92536T1/de

1989-09-06 Priority to SU894742104A priority patent/RU2074392C1/ru

1989-09-06 Priority to HU894650A priority patent/HU204132B/hu

1989-09-07 Priority to JP1232660A priority patent/JP2860118B2/ja

1990-08-15 Publication of DD281659A5 publication Critical patent/DD281659A5/de

1993-01-12 Priority to US08/004,643 priority patent/US5480779A/en

Links

229930182912 cyclosporin Natural products 0.000 title claims abstract description 33
238000000034 method Methods 0.000 title claims abstract description 19
108010036941 Cyclosporins Proteins 0.000 title claims abstract description 14
239000000758 substrate Substances 0.000 claims abstract description 12
108090000790 Enzymes Proteins 0.000 claims abstract description 7
102000004190 Enzymes Human genes 0.000 claims abstract description 7
230000005764 inhibitory process Effects 0.000 claims abstract description 6
ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims abstract description 5
ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims abstract description 5
108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 3
150000001875 compounds Chemical class 0.000 claims description 7
108090000317 Chymotrypsin Proteins 0.000 claims description 5
238000011088 calibration curve Methods 0.000 claims description 5
229960002376 chymotrypsin Drugs 0.000 claims description 5
238000001514 detection method Methods 0.000 claims description 5
229940088598 enzyme Drugs 0.000 claims description 4
101000851058 Homo sapiens Neutrophil elastase Proteins 0.000 claims description 2
102100033174 Neutrophil elastase Human genes 0.000 claims description 2
108091005804 Peptidases Proteins 0.000 claims description 2
239000004365 Protease Substances 0.000 claims description 2
102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 2
108090000190 Thrombin Proteins 0.000 claims description 2
125000000266 alpha-aminoacyl group Chemical group 0.000 claims description 2
229960004072 thrombin Drugs 0.000 claims description 2
102000003813 Cis-trans-isomerases Human genes 0.000 claims 1
108090000175 Cis-trans-isomerases Proteins 0.000 claims 1
102000004195 Isomerases Human genes 0.000 claims 1
108090000769 Isomerases Proteins 0.000 claims 1
230000015572 biosynthetic process Effects 0.000 claims 1
238000006317 isomerization reaction Methods 0.000 claims 1
PMATZTZNYRCHOR-CGLBZJNRSA-N Cyclosporin A Chemical compound CC[C@@H]1NC(=O)[C@H]([C@H](O)[C@H](C)C\C=C\C)N(C)C(=O)[C@H](C(C)C)N(C)C(=O)[C@H](CC(C)C)N(C)C(=O)[C@H](CC(C)C)N(C)C(=O)[C@@H](C)NC(=O)[C@H](C)NC(=O)[C@H](CC(C)C)N(C)C(=O)[C@H](C(C)C)NC(=O)[C@H](CC(C)C)N(C)C(=O)CN(C)C1=O PMATZTZNYRCHOR-CGLBZJNRSA-N 0.000 abstract description 23
108010036949 Cyclosporine Proteins 0.000 abstract description 21
229960001265 ciclosporin Drugs 0.000 abstract description 21
229930105110 Cyclosporin A Natural products 0.000 abstract description 19
239000003814 drug Substances 0.000 abstract description 12
230000000694 effects Effects 0.000 abstract description 11
101000878213 Homo sapiens Inactive peptidyl-prolyl cis-trans isomerase FKBP6 Proteins 0.000 abstract description 10
102100036984 Inactive peptidyl-prolyl cis-trans isomerase FKBP6 Human genes 0.000 abstract description 10
229940079593 drug Drugs 0.000 abstract description 8
238000005259 measurement Methods 0.000 abstract description 6
210000002381 plasma Anatomy 0.000 abstract description 5
238000012544 monitoring process Methods 0.000 abstract description 4
238000003556 assay Methods 0.000 abstract description 2
210000004369 blood Anatomy 0.000 abstract description 2
239000008280 blood Substances 0.000 abstract description 2
210000002966 serum Anatomy 0.000 abstract description 2
238000002560 therapeutic procedure Methods 0.000 abstract 2
239000012620 biological material Substances 0.000 abstract 1
239000000872 buffer Substances 0.000 description 6
238000013459 approach Methods 0.000 description 4
238000006243 chemical reaction Methods 0.000 description 4
238000004364 calculation method Methods 0.000 description 3
239000000203 mixture Substances 0.000 description 3
JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
241000283690 Bos taurus Species 0.000 description 2
IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
239000007995 HEPES buffer Substances 0.000 description 2
108010027597 alpha-chymotrypsin Proteins 0.000 description 2
230000008030 elimination Effects 0.000 description 2
238000003379 elimination reaction Methods 0.000 description 2
230000001900 immune effect Effects 0.000 description 2
210000003734 kidney Anatomy 0.000 description 2
210000000056 organ Anatomy 0.000 description 2
210000000496 pancreas Anatomy 0.000 description 2
230000035945 sensitivity Effects 0.000 description 2
238000000926 separation method Methods 0.000 description 2
239000000243 solution Substances 0.000 description 2
230000003595 spectral effect Effects 0.000 description 2
239000000126 substance Substances 0.000 description 2
208000023275 Autoimmune disease Diseases 0.000 description 1
239000011149 active material Substances 0.000 description 1
239000000427 antigen Substances 0.000 description 1
102000036639 antigens Human genes 0.000 description 1
108091007433 antigens Proteins 0.000 description 1
239000013060 biological fluid Substances 0.000 description 1
210000000601 blood cell Anatomy 0.000 description 1
230000036765 blood level Effects 0.000 description 1
210000001124 body fluid Anatomy 0.000 description 1
239000010839 body fluid Substances 0.000 description 1
210000001175 cerebrospinal fluid Anatomy 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
230000037029 cross reaction Effects 0.000 description 1
238000011982 device technology Methods 0.000 description 1
230000008105 immune reaction Effects 0.000 description 1
238000003018 immunoassay Methods 0.000 description 1
239000003112 inhibitor Substances 0.000 description 1
230000002452 interceptive effect Effects 0.000 description 1
238000004811 liquid chromatography Methods 0.000 description 1
238000004519 manufacturing process Methods 0.000 description 1
239000000463 material Substances 0.000 description 1
238000002414 normal-phase solid-phase extraction Methods 0.000 description 1
125000001151 peptidyl group Chemical group 0.000 description 1
230000000704 physical effect Effects 0.000 description 1
235000015277 pork Nutrition 0.000 description 1
230000002035 prolonged effect Effects 0.000 description 1
230000002797 proteolythic effect Effects 0.000 description 1
238000011084 recovery Methods 0.000 description 1
239000000725 suspension Substances 0.000 description 1
210000001179 synovial fluid Anatomy 0.000 description 1
210000001519 tissue Anatomy 0.000 description 1
231100000331 toxic Toxicity 0.000 description 1
230000002588 toxic effect Effects 0.000 description 1
210000002700 urine Anatomy 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/18—Testing for antimicrobial activity of a material
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/533—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving isomerase
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
- C12Q2337/10—Anilides
- C12Q2337/12—Para-Nitroanilides p-NA

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Zoology (AREA)
Engineering & Computer Science (AREA)
Immunology (AREA)
General Engineering & Computer Science (AREA)
Microbiology (AREA)
Molecular Biology (AREA)
Analytical Chemistry (AREA)
Biotechnology (AREA)
Physics & Mathematics (AREA)
Biochemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Biophysics (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Toxicology (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Measuring And Recording Apparatus For Diagnosis (AREA)
Investigating Or Analysing Biological Materials (AREA)

DD88319577A 1988-09-07 1988-09-07 Verfahren zur konzentrationsbestimmung von cyclosporinen DD281659A5 (de)

Priority Applications (9)

Application Number	Priority Date	Filing Date	Title
DD88319577A DD281659A5 (de)	1988-09-07	1988-09-07	Verfahren zur konzentrationsbestimmung von cyclosporinen
EP89115759A EP0360029B1 (fr)	1988-09-07	1989-08-26	Procédé et essai pour la détermination de la concentration des cyclosporines
ES89115759T ES2058425T3 (es)	1988-09-07	1989-08-26	Procedimiento y ensayo para la determinacion de concentracion de ciclosporinas.
DE8989115759T DE58905125D1 (de)	1988-09-07	1989-08-26	Verfahren und assay zur konzentrationsbestimmung von cyclosporinen.
AT89115759T ATE92536T1 (de)	1988-09-07	1989-08-26	Verfahren und assay zur konzentrationsbestimmung von cyclosporinen.
SU894742104A RU2074392C1 (ru)	1988-09-07	1989-09-06	Способ определения циклоспорина а в крови
HU894650A HU204132B (en)	1988-09-07	1989-09-06	Method and reagent set for detecting concentration of the cyclosporines
JP1232660A JP2860118B2 (ja)	1988-09-07	1989-09-07	シクロスポリンの濃度測定のための方法とキット
US08/004,643 US5480779A (en)	1988-09-07	1993-01-12	Cyclosporine assay

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
DD88319577A DD281659A5 (de)	1988-09-07	1988-09-07	Verfahren zur konzentrationsbestimmung von cyclosporinen

Publications (1)

Publication Number	Publication Date
DD281659A5 true DD281659A5 (de)	1990-08-15

Family

ID=5602261

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DD88319577A DD281659A5 (de)	1988-09-07	1988-09-07	Verfahren zur konzentrationsbestimmung von cyclosporinen

Country Status (8)

Country	Link
EP (1)	EP0360029B1 (fr)
JP (1)	JP2860118B2 (fr)
AT (1)	ATE92536T1 (fr)
DD (1)	DD281659A5 (fr)
DE (1)	DE58905125D1 (fr)
ES (1)	ES2058425T3 (fr)
HU (1)	HU204132B (fr)
RU (1)	RU2074392C1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
RU2128339C1 (ru) *	1997-07-30	1999-03-27	Московский областной научно-исследовательский клинический институт	Способ определения циклоспорина ав крови

1988
- 1988-09-07 DD DD88319577A patent/DD281659A5/de unknown
1989
- 1989-08-26 EP EP89115759A patent/EP0360029B1/fr not_active Expired - Lifetime
- 1989-08-26 AT AT89115759T patent/ATE92536T1/de not_active IP Right Cessation
- 1989-08-26 ES ES89115759T patent/ES2058425T3/es not_active Expired - Lifetime
- 1989-08-26 DE DE8989115759T patent/DE58905125D1/de not_active Expired - Lifetime
- 1989-09-06 RU SU894742104A patent/RU2074392C1/ru active
- 1989-09-06 HU HU894650A patent/HU204132B/hu unknown
- 1989-09-07 JP JP1232660A patent/JP2860118B2/ja not_active Expired - Lifetime

Also Published As

Publication number	Publication date
RU2074392C1 (ru)	1997-02-27
ATE92536T1 (de)	1993-08-15
HU204132B (en)	1991-11-28
ES2058425T3 (es)	1994-11-01
EP0360029B1 (fr)	1993-08-04
EP0360029A1 (fr)	1990-03-28
DE58905125D1 (de)	1993-09-09
JPH02150298A (ja)	1990-06-08
JP2860118B2 (ja)	1999-02-24
HUT54809A (en)	1991-03-28

Legal Events

Date	Code	Title	Description
1994-06-23	RPI	Change in the person, name or address of the patentee (searches according to art. 11 and 12 extension act)
1998-07-02	RPI	Change in the person, name or address of the patentee (searches according to art. 11 and 12 extension act)
1998-07-02	RPV	Change in the person, the name or the address of the representative (searches according to art. 11 and 12 extension act)
2004-04-28	ASS	Change of applicant or owner	Owner name: NOVARTIS AG, BASEL Effective date: 19980430
2004-04-28	IF04	In force in the year 2004	Expiry date: 20080908

Publication	Publication Date	Title
EP0158254B1 (fr)	1988-06-08	Réactif pour la détermination du temps de thromboplastine
DE2548963A1 (de)	1977-05-05	Verfahren und mittel zur bestimmung der aktivitaet von creatinkinase-mb
DE2830862C2 (fr)	1988-06-01
Chayen et al.	1976	The cytochemical bioassay of hormones
EP0001223A2 (fr)	1979-04-04	Latex recouvert d'un composé polyhydroxy, procédé de préparation de ce latex, réactif immunologique comprenant ce latex, procédé de préparation de ce réactif, application de ce réactif, procédé d'analyse utilisant ce réactif et trousse contenant ce réactif
EP0174652A2 (fr)	1986-03-19	Procédé de mesure immunochimique pour haftènes et protéines
WO2009006877A2 (fr)	2009-01-15	Détermination de l'activité de protéases
EP0559853B1 (fr)	1996-10-16	Procede de stabilisation de peptides endogenes physiologiquement actifs
JP4105768B2 (ja)	2008-06-25	タンパク質の抽出方法
GB1569392A (en)	1980-06-11	Method and reagent for the determin. of antithrombin iii
Yoder et al.	1977	Bioassays for detection and quantification of Helminthosporium maydis race T-toxin: a comparison
EP0360029B1 (fr)	1993-08-04	Procédé et essai pour la détermination de la concentration des cyclosporines
DE60225587T2 (de)	2009-04-02	Protease assay zur kontrolle medikamentöser therapie
Lorenz et al.	1987	Reliability and practicability of the fluorometric-fluoroenzymatic histamine determination in pathogenetic studies on peptic ulcer: detection limits and problems with specificity
Al-Khalidi et al.	1970	A method for the determination of plasma guanase on finger-tip blood
EP0148193B1 (fr)	1987-08-12	Procede et reactif pour la determination d'"angiotensin converting enzyme"
US3715188A (en)	1973-02-06	Cholesterol assay and reagents therefor
US4615877A (en)	1986-10-07	Method of determining the oxidizing activity of a biological liquid, and a corresponding reagent
RU2164026C2 (ru)	2001-03-10	Способ определения состояний шока и контроля за ходом лечения пациентов в состоянии шока
DE69628878T2 (de)	2004-05-27	Methode zur Bestimmung von Cholinesterase und Methode zur Unterscheidung zwischen Leberzirrhose und Hepatitis
DE19719001C2 (de)	2001-05-10	Verfahren zum immunologisch-funktionalen Nachweis biologisch aktiver Insulin-Like Growth Factor Binding Proteine oder löslicher Insulin-Like Growth Factor - Rezeptoren im Immunoassay
Khokher et al.	1983	Human immunoglobulin G stimulates human adipocyte lipogenesis
Lott et al.	1972	Determination of Total, Ionic Serum Calcium
Norkus et al.	1976	Four commercial urease reagents and a laboratory-prepared reagent compared for analysis of blood urea nitrogen with the Beckman analyzer.
SU1429020A1 (ru)	1988-10-07	Способ определени проницаемости мембраны эритроцитов и концентрации кальци в цитоплазме