DD282706C4 - METHOD FOR PRODUCING A BIOPHYSICALLY DERIVATIVED HEFEPRAEPARATE WITH A BREATHABLE ACTIVITY, THESE INGREDIENT AND PLANT GROWTH SUBSTANCES, AND THEIR USE - Google Patents

Abstract

A biophysically derived yeast preparation is proposed which stimulates respiration in liver homogenate, contains an L-amino acid oxidase inhibiting factor, and exhibits excellent cicatrization properties and RES-stimulating action. The process for manufacturing the yield preparation consists in treating an initial yeast, if necessary with addition of a liquid support, until a light resuspended material is obtained, followed by a brief thermal shock treatment until gases are evolved by fermentation. In addition, fodder and plant hormone solutions containing these biophysically derived yeasts are proposed.

Description

For this 2 pages drawings

Field of application of the invention

The invention relates to processes for the preparation of a metabolism-activating preparation, to feeds and plant growth substances containing them and to the use of the preparations for skin treatment and for probiotic activation.

Characteristic of the known state of the art

It is known that plant and animal organisms can influence and activate cell metabolism. On this basis, numerous metabolic activation methods have already been proposed. According to DE-PS 1076888, respiratory active substances are obtained from dialyzed blood cells or blood plasma; they should increase the oxygen uptake of rat liver homogenate. Growth-promoting products are obtained according to USP 3,937,816 from the red pulp of bovine spleen and causes a spleen weight gain in mice after intravenous injection in a relatively short time. Information on respiratory activity or effects on respiratory chain phosphorylation is not provided.

It is also known that therapeutically active substances can be enriched in a yeast (see also DE-OS 3341840).

Object of the invention

The object of the invention is to increase metabolic activity, to improve wound healing, to positively influence the reticuloendotholial system and to stimulate growth.

Explanation of the essence of the invention The invention has the object of physically modifying the biochemistry of the yeast products. According to the invention, this object is achieved in that the preparation of biophysically derivatized yeast with

breathable activity measurable in the Warburg tube on standardized rat liver homogenate and expressed as the additional oxygenate volume inhaled by the homogonate. In a preferred embodiment of

Invention is the respiratory Stelgerungsfaktor at 37 ⁰ C at least 2.0, based on the homogenate, otherwise

same experimental conditions.

The respiration rate factor at 37 ^° C. has a value of 2.0 to 10, preferably a value of 2.5 to 6.5. In a further preferred embodiment of the invention, the preparation contains an L-amino acid oxidase inhibiting factor,

measurable in Warburg vessel on a standardisable test solution with the enzyme and phenylalanine as L-amino acid and expressed as ratio of oxygen consumption with and without yeast preparation. The L-amino acid oxidase inhibitory factor has a value in the range of about 20 to 80% and preferably a value in the range of 30 to 60%.

Another feature provides that the preparation has a wound-healing activity and additionally a RES-activating Effect shows. Furthermore, it affects the growth of yeasts and microorganisms, e.g. Bifidus, Lactobacillus, Cocci and Soil bacteria are positive and thus indirectly the growth of higher plants and algae. In a further embodiment, the preparation additionally contains extracted from animal and / or plant cells Mitochondrion. The object is further achieved in that starting yeast, optionally with the addition of a liquid carrier Exposure to laser radiation, freeze-drying, ultrasound treatment, brief pressurization and / or Osmolyse be treated until the cells are converted into a slightly resus-pendierbare mass and the Suspension of contracted duties is subjected to a short-term intensive heat shock treatment until fermentation

escaped gases escape.

In a preferred feature, the heat treatment of the cells is no longer than 26 minutes at a temperature above 40 ° C. The heat treatment is carried out directly with a countercurrently fed hot gas. The biophysical cell reduction is carried out simultaneously with the brief intense heat. When

liquid carrier is added to an aqueous solution or suspension containing

1. 0.1 to 0.6% biophysical derivatized yeast obtained either from one yeast used as starting material and / or from another yeast and from 0.02 to 0.4% chondriosomes extract,

2. 0.3 to 5% cellulose glycolate, fumed silica with more than 99.8% SiO ₂ and a diameter of 7 to 25 nm, carrageenan, trayanth or similar fillers,

3. 0.01 to 0.3% fermentable mono- and / or disaccharides,

4. Preservatives such as parabens and salts such as saline in sufficient concentration,

5. trace elements such as Mn, Co, Zn, Mg, preferably as sulfates or gluconates.

Another feature provides that a strain from the family of Saccharomycetaceae is used as starting material. In a further feature of the method according to the invention is a dry yeast with a HTS content of 95 to 98%

treated with 2 x to 4 times the amount of a carrier.

The object is further achieved in that a conventional feed is used, the up to 1 wt .-% of biophysical

derivatized preparation having breath-increasing activity according to one of the aforementioned features.

The solution of the problem is also that a Pflanzenwuchsstofflösung is used, which consists of irrigation water and up to

2Gew .-% of the biophysically derivatized preparation according to one of claims 1 to 18.

The biophysically derivatized preparation is used for skin treatment or skin conditioning, preferably for Normalization and increase of the moisture retention capacity, the increase of the elasticity of the skin, the wound healing of Skin or in combination with known skin-healing and / or skin conditioning agents such as collagen, Organo-extracts, plant extracts or polyhydric alcohols containing from 2 to 8 carbon atoms. The biophysically derivatized preparation is further used for prc biotic activation of dairy products, wherein

the derivatized cell material interacts with selected natural microorganisms in a growth-stimulating manner.

The preparation is preferably used in yoghurt production or for growth stimulation of Lactobacillus

bulgaricus.

The biophysically derivatized preparation is also used for probiotic activation of Actinomyceten, in particular for Activation of yeasts, preferably yeasts of the groups Aspergillus niger, Torula utilis, Menrospora crassa, Saccharomyces species and Japanese Ikashiokara yeast. Algae, fungi and yeasts having an increased metabolic activity such that they are used in rat liver homogenate

have a respiratory enhancing effect, were previously unknown. The conventional yeasts, such as brewer's yeast,

Saccharomyces cerevisiae and Saccharomyces carlsbergiensis, baker's yeasts (Saccharomyces), Candida, Torula et al. a. behavior

inert to such a homogenate. The respiratory increase factor is essentially one.

The technical-economic effects of the invention, in particular their effectiveness, consists in the increase of Metabolic activity and in addition to the increase in respiration of organ homogenates in an improved wound healing. A growth-stimulating effect and the positive influence on the reticuloendothelial system was found in fish,

but also found in warm-blooded animals.

The metabolic active Ascymycetes, Schizomycetes and yeast preparations of the invention also affect growth

normal yeasts and bacteria of the Lactobacillus and Bifidus species are positive.

In addition, most Ascomycetes, Schizomycetes and yeast preparations of the invention have an inhibitory factor,

which acts to inhibit the oxidases.

embodiment The invention will be explained in more detail below with reference to several examples. In the accompanying drawings shows

Fig. 1: a two-dimensional TLC diagram for lipids and other ingredients of a derivatized yeast, Fig. 2: Traces for treated and untreated skin.

The term "Ascomycetes" in the present description, the mushrooms with tubular hyphae and Ascospora formation, which also includes the yeasts (Saccharomyces), and include the yeast-like fungi, their sporulation

still unknown, z. B. the genera Candida and Torulopsis. "Schizomycetes" are yeast-like bacteria In the description, the term "mushrooms" is used abbreviated for the aforementioned genera.

"Yeast preparation" in the present specification means farmyard gums, yeast solution, yeast suspensions, including

Yeast extract. The respiratory enhancement factor for the metabolically active yeast preparations of the invention is determined by the effect Rat liver homogenate according to the Warburg methodology and determines the ratio of standardized Rat liver homogenate additionally breathed oxygen (volume), based on a drug-free control otherwise

of the same composition, dar.

The measurement of the increase in respiration and the respiratory increase factor takes place in a Warburg vessel Volume containing a buffer (preferably pH 7.4 Sörensen buffer), a certain amount of conditioned Rat liver homogenate and the test substance or test solution is filled according to a filling plan and in which the Test substance usually over a period of 60 min at 37 ⁰ C acts on the homogenate. By reading the pressure gauge

Oj consumption is determined after CO ₂ produced by respiration has been quantitatively adsorbed. The oxygen consumption converted to standard conditions, divided by the consumption of a control sample without test substance, is a direct measure of the respiration increase factor.

The measurable increase in respiration in the Ascomycetes, Schizomycetes and yeast products of the invention is dramatic, as can be seen by the following Table I with a yeast product according to Example 1.

Table I

No sample Respiratory increase factor percentage increase (%) 500 750 WSR (%) 4.4 4.0 2.8 1.0 340 300 180 40 52 50 1 complete solution 2 centrifuge supernatant 3 centrifuge residue 4 starting material 30 Table II Yeast cells seeding (cells / 10ml) Sowing WSF (cells / 1 OmI) (%) yeast 2.0 x 10 "2.0 x 10 ^β 1,0-10 '1,5-10' a) Sabouraud Maltose 2% Bouillon Normal yeast Normal yeast + 2% Comp. yeast product 5.0 · 10 "5.0 · 10" 2.0 x 10 '2.6 x 10 * b) Saboraud maltose 2% bouillon normal yeast normal yeast + 2% comp. yeast product

WSF = growth factor WSR = growth rate Normal yeast (a) = Saccharomyces cerevisiae Normal yeast (b) = Saccharomyces carlsbergiensis A similar positive effect on growth was also found in bacteria such as Lactobacillus and Bifidus species. In view of the altered biochemistry of the derivatized starting products based on Ascomycetes, Schizomycetes

and yeast cells are the TLC diagrams of lipids, amino acids and. obtained under defined experimental conditions

Nucleic acid Kciponenten compared to the starting cells used for derivatization of interest. The obtained two-dimensional TLC diagrams are suitable for the characterization of the derivatized products and their Differentiation of differently manufactured products. Figure 1 shows a two-dimensional TLC plot of lipids and other ingredients of a derivatized yeast. For this was

1 g of derivatized yeast is extracted with 6 ml of CHCl ₃ / CH ₃ OH (2: 1).

The extract was chromatographed:

1. flow agent CHCl ₃ = CH ₃ OH = H ₂ O (65: 25: 4)

2. Solvent C _e H _e : C ₄ H ₁₀ O: C ₂ H _e O: CH ₃ COOH (65: 40: 1: 0.5) Benzene: Diethyl ether: Ethanol: acetic acid

To detect the separated ingredients, the plates are first exposed under a UV analysis lamp at 366 nm and 254 nm

considered and the spots are marked. To detect lipids, the plates are sprayed with Primulin reagent and evaluated under the UV analysis lamp at 366 nm.

Surprisingly, most of the ascomycetes, schizomycetes and yeast preparations according to the invention have been used

in addition to the respiratory enhancing activity, a inhibitory factor which inhibits the oxidases is found. When

Oxidases are especially called the amino acid oxidases [1.4.3.2. or 3] and the monoamine oxidases [1.4.3.4] and the Diaminooxidases [1.4.3.6]. Triggered by this effect are probably the oxidation of L-amino acids, which in themselves are caused by L-amino acid oxidation

[1.4.3.2.] Is catalyzed, inhibited or slowed down. L-amino acid oxidase is common in many nutrient utilization systems

Intestinal flora and soil cultures included. Direct effect of this inhibitory factor is a higher percentage of L-amino acid Use in the diet, which is fed to such a nutrient utilization system, with the result that the animal or dio Plant can grow faster. Whether this inhibitory factor is directly related or at least correlated with increased respiratory activity,

is not yet secured.

Without specifying any mechanism here, the effect of this inhibitory factor could be explained as follows: As a prosthetic group, L-amino acid oxidase contains firmly bound FMN (flavin mononucleotide). In the oxidation of L-amino acids whose amino group is oxidized according to the following scheme:

1) L-AS + H ₂ O / enzyme FMN - »α-keto acid + NH ₃ + enzyme-FMN-H ₂

2) Enzyme-FMN · H ₂ + O ₂ - ♦ Enzyme-FMN + H ₂ O ₂

The oxygen consumption may be measured taking into account the formed H ₂ O ₂ amount, since H ₂ O ₂ '/ 2O ₂ disintegrates in water and. In the presence of catalase, this decay is spontaneous and quantitative, so that the overall reaction of L-amino acid oxidation is Equation 3.

3) R-CH-COOH + '/ 2O ₂ - * R-CO-COOH + NH ₃ NH ₂

If L-amino acid oxidase is inhibited or blocked or otherwise immobilized in a system, less oxygen will be produced

consumed as without this inhibitory factor, leaving more amino acids and proteins available, which can lead to increased and more intensive nutrient utilization.

For the determination of the L-amino acid oxidase inhibitory factor (Hmf) of the Ascomycötes, Schizomycetes- and Yeast preparations are L-amino acid oxidase solution (1 mg / ml), catalase suspension, buffer pH 7.8 (SOmI 0.2 M Na ₂ P ₂ O ₇ + 37.5 ml 0.2 M HCl). L-phenylalanine solution (1 mmol / 50 ml, buffer pH 7.8) and test solution (centrifugate, 10 ⁶ g; Yeast suspension) after a certain filling plan in a Warburggefäß filled, so that the measurements both the implementation

of L-amino acid oxidase and! .- Phenylalanine and the reaction of the enzyme with test solution (without phenylalanine) and the

Enzyme with test solution and phenylalanine capture. The cylinder inserts are provided with filter paper strips with

6 N KOH are soaked. The solutions are equilibrated at 37 ⁰ C, then the enzyme solution in the test solution or a

Blind solution containing main space given to start the reaction. The μΙ consumption of oxygen can then be in Dependence on time can be plotted and is a direct measure of the inhibitory factor when dealing with the Oxygen consumption of the blind tests correlated. In general, the reading used to determine the Hmf will follow

60min.

For evaluation, the sum curve of the measurement curves "L-amino acid oxidase + phenylalanine" and

"L-amino acid oxidase + yeast solution" - the oxygen demand is due to the amino acids contained in the yeast itself - and then sets the Summon curve, which is a measure of the theoretically expected oxygen uptake, in

Relationship with the curve "L-amino acid oxidase + L-phenylalanine + yeast solution." In the case of the ascomycetes, Schizomycetes and yeast preparations (biophysically derivatized) results in an under-consumption of oxygen, the

percent inhibitory factor (eg 0.42 = 42% inhibition or less oxygen consumption). Compared with the respective starting cell materials, which correspond practically to the theoretical cumulative curves, the Hmf is considerable and reaches values of up to 70 or even 80%.

Another surprising feature of most Ascomycetes, Schizomycetes and yeast preparations according to the invention is

their wound healing activity, which can be evaluated in the healing of cuts on the back of rats. The tension at the wound site of the treated animals was significantly greater after β days than in a control group. Also at the test on the 21st day the lead of the treated animals before the control group was still measurable.

In this test, male rats of the Wistar breed weighing 210-23Og were anesthetized, and under Anesthesia, the back hairs were shaved. Then along the media line a straight cut of about 4cm in length

attached and sewn together at intervals of 1 cm in three places. On the 6th, 11th, 16th and 21st day after the procedure, the wound was opened and 3 small slices of skin were taken at right angles to the wound line, including the 1 cm width of the wound line. Subsequently, the tension was measured, which resulted from the removal of the batch used.

2.5 ml / kg of the centrifuge supernatant of the test solution of Example 1 were administered on the day of the wound incision and then daily. The control group received an application of 2.5 ml / kg of physiological saline in otherwise the same

Wise. Each group tested 25 animals per group. It was observed that the tension of the wounded area of the skin in the animals treated with the test solution

increase evenly. At each day test, the treated animals showed a higher tension of the skin area than the

Control group. In the initial stage on the 6th day the difference to the control group was greater than 100%. The results are in Tab. Ill summarized.

Table III Test Group * control group Number of days 215 ± 17 493 ± 24 840 ± 48 1 659 ± 52 104 ± 33 390 ± 23 730 ± 30 1401 ± 60 6 11 16 21

* Test group received 2.5ml test solution / kg body weight

The markedly improved wound healing can be explained so that the Ascomycetes, Schizomycetes and yeast preparations according to the invention promote tissue ventilation and increase the metabolic function so that the tissue is regenerated much more rapidly.

Not only the injured skin, but also healthy, uninjured skin by the preparation according to the invention, for. As the dervated yeast, favorably influenced. Under the influence of a biophysically derivatized yeast preparation (Example 1), the moisture retention capacity of human skin was normalized and improved many times. To demonstrate this effect, the water content of the skin was determined before and after the treatment over several hours. Preparation containing formulations were applied to a dry skin, so on a skin with poor moisture balance. On the same skin, a control sample without preparation, but otherwise the same composition was abandoned. As a result, it was found that the water content was increased and the moisture retention capacity of the skin was practically doubled. FIG. 2 shows the progress curves for treated and untreated skin. On the X-axis are the hours after treatment and on the Y-axis is the moisture of the skin in percent.

As already explained with reference to wound healing, skin elasticity or skin tension is improved by formulations containing a fungus or yeast preparation according to the invention, which can be easily followed by resonance frequency measurements on human skin.

Another criterion for the increased metabolic activity of the Ascomytes-, Schizomycetes- and yeast preparations according to the invention is the positive influence on the reticuloendothelial system. Due to its effect on the inhibited by Tryphanblau function of RES rattan according to the Congo red method can be found for the new preparations, a RES-activating effect // earth. For this purpose, male Wistar rats having a body weight of 210-23 Og were injected intravenously with 1 ml / kg of a 1% Congo red solution; after 4 minutes and after 60 minutes blood was taken. The Congo red coefficient was the ratio of the absorbance at 500 nm of the tenfold diluted serum. To inhibit the function of the RES system, 10 ml / kg of 1% tryphan blue solution was administered intraperitoneally. By measuring the absorbance at 650 nm, the inhibition could be calculated as a corrected decadic absorbance at 500 nm. Tryphan Blue was administered 6.5 hours prior to examination of the RES system with Congo Red. In the test, 0.5 ml of the test solution (centrifugal supernatant naci, Example 1) was intraperitoneally administered 5 hours before this examination. The control group received adequate Menyo physiological saline. 25 animals were tested per group.

For the treated group, an average Congo red coefficient of 9.4 and for the control group a Congo red coefficient of 16.1 was found, which is a direct demonstration of the enhancing effect of the Ascomycetes, Schizomycetes and Yeast preparations of the invention on function of the RES system, since the inhibitory effect is reversed by tryphan blue.

The increased metabolic activities demonstrated for the Ascomycetes, Schizomycetes and yeast preparations according to the invention are completely surprising insofar as the Ascomycetes, Schizomycetes and yeast cells and their cell preparations are treated only by physical methods. This treatment is generally called "derivatization." Ascomycetes, Schizomycetes, and yeast cells can be derivatized by exposure to cold, laser irradiation, sonification, and other biophysical methods, as well as relatively brief intense heat treatment, which obviously results in a marked reduction in size of the cells Yeast cells are preferably 0.5 times or less as the cell walls become stronger, so that the ratio of mean cell diameter / cell wall thickness is changed by a factor which is preferably in the range of about 5 to 20.

The preparation of Ascomycetea and Schizomycetes cell materials preferably additionally contains a smaller amount of mitochondrial extract, by means of which the growth-stimulating activity of the preparation can be additionally increased. The mitochondrial extract is best added before or during the biophysical treatment. Mitochondrion (also called chondriosomes) are variable in shape, about 0.1 to 1 pm wide and 1-5 pm long inclusions in the protoplasm of cells of unicellular organisms, plants and animals. They are commercially available as pyrogen-free dry powders or as solutions, such as. B. Mitochondyl powder Fa. Widmer AG, Switzerland, which has about 2% nitrogen and a dry matter content of about 50%. This Handols product is used in the examples. In general, the mitochondrial extracts are prepared by shattering cells, mostly yeast cells, and subsequent appropriate rehydration, suspension, washing and drying steps. See, e.g. P. M. Nossal, Austral. J. exp. Biol. Med. Be. 31,583 (1965); M. Merkerschläger, K. Schlossmann, W. Kurz, Biochem. Z. 329,332 (1957). Miiochondyl is a product of extracted yeast cell mitochondria mixed with buffering agents and is added as a white powder directly or by preparation of a corresponding buffered solution which also contains preservatives. Preferably, a mitochondrial extract amount of about 0.05 to 0.50 wt% is used, expressed as dry substance and based on the dry solids content of the cells to be derivatized.

An increase in the Ascomycetes, Schizomycetes and yeast cells under the given conditions during the treatment is practically no longer taking place and is not desirable

 The preferred carrier used is:

a) a small amount (preferably 0.2 to 5.0% by weight) of already biophysically derivatized yeast and / or chondriosome extract (preferably 0.01 to 0.1% by weight), obtained from the yeast used as starting material or another yeast,

b) cellulose glycolates, fumed silica, tranganth and / or carrageenans,

c) two fermentable mono- and / or disaccharides ^

d) preservatives,

c) trace elements such as Mn, Co, Zn, Mg, as sulfates or Giukonate.

The starter yeast (a) serves as a starter for the treatment, the additive (b) ensures a better distribution of the yeast. The carbohydrates (c) protect against the fermentation of the cell's own carbohydrates. As preservative (d) are preferably used parabens (4-Hydroxybenzoesäurealkylester), saline and calcium chloride. They protect the end product from the onset of exposure to microorganisms. The addition of trace elements (e) allows for desirable concentration shifts in the yeast cell or yeast cell suspension.

The growth of microorganisms z. B. bifidus, lactobacillus, cocci, soil bacteria and intestinal bacteria, is positively influenced by the Ascomycetes ·, Schizomycetes and yeast products of the invention.

With the biophysically derivatized Ascomycetes®, Schizomycetes® and yeast preparations according to the invention, a marked influence on plant growth, inflorescence and fruiting is achieved in higher plants. If up to 1% of the liquid Ascomycetes®, Schizomycetes® and yeast preparations according to the following Examples 1 to 6 are added to the irrigating water, ornamental plants such as Tagetes, Petunia, Lobelia, Chrysanthemums and Cloves grow over a test period of six weeks compared with a control sample at least twice as fast with pronounced leaf and flower abundance. Similar improvements are also observed in crops such as peas, sunflowers and corn, thus demonstrating the excellent suitability of the preparations according to the invention as a growth-stimulating additive to casting and irrigation solutions. This effect is likely to be explained by a favorable influence on the microflora of the soil.

example 1

30 parts by weight of 95% dry yeast (Saccharomyces cerevisiae) and 70 parts by weight of an aqueous solution or suspension of a carrier containing (by weight) 0.5% biophysically derivatized yeast, 0.03% chondriosomes extract, 0.6% cellulose glycolates, 0, 1% parabens, 2% salt, 0.1% total glucose and sucrose, 0.00001% trace elements are irradiated at a temperature of 15-25 ° C with a conventional CO ₂ laser. The power of the laser in the order of 0.3 watts is quite sufficient. Its focus is 0.05-5kW / cm ² .

For the recording of the suspension for irradiation with the CO _r laser, a device is used, which consists of two vessels of suitable size, which are connected to each other at the lower ends by an existing of infrared glass P / · γ. This tube has a length of 20-50cm and an inner diameter of 3-6mm. 2Ol of the suspension are pressed with pure nitrogen into the nitrogen-flushed left vessel of the device. After the laser has been set with the endoscope to a point size corresponding to the inner diameter of the connecting tube, the suspension is pressed with nitrogen from one vessel into the other vessel, wherein the second vessel is placed under nitrogen. At a power of 0.3 W and a flow rate of the suspension of 20-200 ml / min is treated until the desired, microscopically verifiable cell reduction. Subsequently, the suspension is briefly (at most 25 min) heated to a temperature above 5O ⁰ C and kept under stirring at the final temperature. After cooling to 3O ⁰ C suspension is then added to preservation with 0.1 to 0,2Gew .-% of a preservative such as sorbic acid and / or Nipagin. The morphological examination of the cells showed a reduction of the average cell diameter to about 50% and a significant strengthening of the cell walls. Both the total product (suspension) and the fractions obtainable by centrifugation (10000 rpm), ie centrifuge supernatant or centrifuge residue, are found to be metabolically active (as opposed to starting yeast) in the Wr.burg trial when respiration-enhancing activity in rat liver homogenate has been demonstrated in the test described was tested. The respiration increase factor for the total product and for the centrifuge supernatant was greater than 4 (see Table I). Further activities were demonstrated in the described wound healing test (Table IiI) and in the test for activating the RES system.

In a dosage of 2% o, the total product was tested in the Vormast of 10-35kg weighing piglets or weaned piglets (see Tab. IV or V). The improvement in daily feed intake was significantly higher at around 5 lb% and 42%, respectively, than when using commercial feed supplements, resulting in an improvement in feed conversion of 16.7%. The results are summarized in detail in the following Tab. IV and V.

Table IV Piglet rearing (10-35 kg live weight)

control commercial preparations B 24 test (without) A 10.3 preparation (2% o) Number of animals / experimental group 24 24 35 24 Initial weight (kg) 10.2 10.1 10.2 Test duration (days) 35 35 30.2 35 Weight at 569 End of experiment (kg) 25.4 26.7 19.9 34.2 daily gain (g) 434 474 131 686 Weight gain (kg) 15.2 16.6 1093 24.0 relative difference (%) 100 109 158 Food Consumption / Animal / Day (g) 908 971 1.02 1194 Feed cost / kg 92 Increment (kg) 2.09 2.05 1.74 relative difference (%) 100 98 30.9 83.3 Improvement: 8th daily increase (%) - 9.21 57.9 Feed conversion (%) - 2 16.7

  The trade preparations were A: CTC and B: bayo-nox (also in Tab.V)

Table V Attempt with weaners

(Weaning age 21 days, live weight 5-12.5 kg)

control commercial preparations B test (without) A 24 preparation (2% o) Number of animals / experimental group 24 24 5.12 24 Initial weight (kg) 4.93 6.14 20 5.06 Versurhsdauor (Taga) 20 20 11.9 20 Weight at the end of the experiment (kg) 10.2 11 6.78 12.9 Total weight gain (kg) 5.23 5.84 339 7.45 daily increase (g) 262 292 130 373 relative difference (%) 100 112 480 142 Food Consumption / Animal / Day (g) 429 465 1.42 492 Fodder wall / kg increment (kg) 1.64 1.59 86.3 1.32 relative difference (%) 100 97 80.5 Improvement: 29.6 daily increase (%) - 11 13.7 42.4 Feed conversion (%) - 3 19.5

Example 2

Add 1001 of a suspension of Torulopsis utilis and Saccharomyces cerevisiae (2: 8 mixture) with a total dry solids content of 20% to a mixture containing:

1 kg of product from Example 1

0.05kg chondriosome extract

0.1 kg cellulose glycolate

0.1 kg of gum arabic

0.1 kg of glucose + mannose

0.1 kg parabens

1.5 kg of a salt mixture of common salt, MnSO ₄ and MgSO ₄ and

0.00001% trace elements.

The suspension is stirred and sonicated at 20 ° C and during the SoniFizierung first to 45 ⁰ C and then

briefly (2 min) heated to 8O ⁰ C.

The cooled suspension is mixed with 200g of silica (Aerosol 300) and enough nipagin (preservative) in one

suitable device (e.g., Büchi apparatus) is spray dried, with about 1.5 kg of solid product per hour.

The respiratory enhancement factor when tested for breath-increasing activity on rat liver homogenate was 5.1, tested on

liquid product (before spray drying). In the wound healing trial, a lead of about 100% over one

Control group found when - as described - the tension of the skin was measured. The starting yeast was

In contrast, inactive.

The increased metabolic activity of the preparation was shown in growth experiments on fish and chickens. Already very

small amounts of the addition to the normal diet (additions less than 1 %) caused a surprisingly high growth effect, which resulted in a considerable feed saving. Tables Vl and VII summarize the results.

Table VI Growth attempts with swordfish Control addition 0.80% o addition 0.95% o

average weight 1.9 3.7 4.1

the young fish after 47 days

At the time of the experiment, 60 juvenile fish were divided into the three groups on the 15th day and added in an amount of 5I water

a mean pelvic temperature of 24.5 ⁰ C fed twice a week. Two fish groups received 0.8 and 0.95%, respectively, of the preparation of Example 2, distributed over 3 doses per week.

The weight gain is extraordinary and proves the growth promoting effect of the drug. The spray dried product of Example 2 was tested as an adjunct to the feed of Harvard chickens. One out

3600 chickens experimental group was fed 60 days under 0.1 wt% additional enrichment. A control group consisting of 3,600 chickens received feed without additive. Body weights of 20 chickens each and mortality were determined (Table VII).

Feeding experiments in chickens 1 10 20 30 40 50 60 Day number: 34 39 209 246 568 650 1110 1100 1590 1510 2001 1890 2090 1990 Weight of the experimental group (g) Weight of the control group (g)

Hluraus gave a food consumption (kg) per kg body weight for the experimental group of 2.27 and for the control group of 2.65. The mortality in the experimental group was 4.9%, that of the control group 7.7%. The improved feed conversion corresponds to a feed saving of about 15%.

Example 3

10 kg pressed yeast (Saccharomyces cerevisiae) are cooled in a suitable cooling device to a temperature below -13 ⁰ C and about 4 hours. conditioned at this low temperature. Then be added to the product 500ml of an aqueous suspension containing 15 g of fructose and sucrose, 40 g of finely divided silica (SiO _2), 50 g of preparation from example 2 (based on solid product) containing 15g ^D arabene and 160a Kcchsalz; the mixture is first intimately mixed and on

23 ° C) to form a liquid mixture in which the yeast particles are easily suspended. The mixture is then heated rapidly in such a manner that the temperature rise is about 5 ° C / 3 min. The mixture will be up

heated to a temperature between 45 * C and 75 "C, while stirring constantly.

It forms a thin yeast suspension with slightly resuspendierbaren particles that as a total solution, but also as Centrifugate (supernatant or residue) has a respiratory increase factor of 4.8. Example 4

10kg pressed yeast (Saccharomyces cerevisiae) are brought to a temperature below -15 ⁰ C. After 24 hours 10 g parabens, 5 g sorbic acid, 170 g NaCl as preservative and 10 g sucrose, 15 g SiO ₂ , 10 g cellulose glycolate and 0.8 mg trace elements (Mn, Co, Zn, Mg) are added.

After the mixture has reached room temperature (25 ^° C.), it is stirred into the laser apparatus described in Example 1 and treated with laser radiation until the yeast particles have shrunk to at least 50% of their diameter before the action of the laser. The mixture is then heated with continued stirring for a short time (at most 20 min) until a temperature between 40 ° C and 8O ⁰ C is reached.

The respiratory enhancement factor of this product is 5.1. After preserving the suspension is spray-dried. The product can be used as a biophysically derivatized starter yeast in Examples 1-3. The product obtained was used for feeding experiments, where the metabolic increasing activity was comparable to that of Example 2 when tested as a feed additive in Harvard chickens. In practice, this activity amounts to a noticeable saving of feed and a much shorter feeding time, which pays off especially in large-scale experiments.

Even when feeding fur animals such as chinchillas, the preparation proved to be extremely useful. The coat of hair is smoother and shinier after a short time of use of drug. The coat of older breeding animals is still usable if they have received the preparation made of yeast for some time.

Example 5

500kg of 20% yeast (Saccharomyces), so-called 20% yeast, were fermented at 20 ° C with 0.2kg parabens, 0.03kg sorbic acid, 2kg NaCl, 0.2kg sucrose, 0.5kg SiO ₂ , 0.3kg cellulose glycolate, 0 , 5kg mitochondrial extract and 10mg of trace elements and stirred with an Ultraturas as a stirrer so strong that the mixture warmed rapidly. At 55 ⁰ C to 6O ⁰ C was stirred for about 10min and then cooled. In feeding trials, comparable test results were obtained as in Examples 1 and 2.

Example 6

50kg yeast 20 (Saccharomyces) were added at 20 ° C with 20g glucose and fructose (10: 1), 40g alginate, 30g mitochondrial extract, 180g NaCl, 10g sodium ascorbate as preservative and 1.5mg trace elements and stirred with the Ultraturrax with simultaneous dusk, of which the mixture without external heat supply heated and at 52-6O ⁰ C short time, but was held for no longer than 20 minutes. The cooled mixture was used to increase yoghurt production with the cultures added less than 0.1% by weight based on the weight of the total medium. The product was also useful for other concerns in the dairy industry as it accelerates the growth of the corresponding microflora.

Claims (21)

1. A process for preparing a biophysically derivatized yeast preparation having breath-increasing activity, characterized in that a starting yeast, optionally with the addition of a liquid carrier, by the action of laser radiation, freeze-drying, ultrasound treatment, brief pressurization and / or osmolysis is treated until the yeast cells in void readily resuspendible mass have been converted, and the suspension of the contracted yeast cells is subjected to a short-term intensive heat shock treatment until gasses formed by fermentation escape. 2. The method according to claim 1, characterized in that the heat treatment of the cells is not longer than 25min at a temperature above 4O ⁰ C is performed. 3. The method according to claim 1, characterized in that the heat action is performed directly with a hot gas fed in countercurrent. 4. The method according to any one of claims 1 to 3, characterized in that the biophysical cell reduction is performed simultaneously with the short-term intensive heat. 5. The method according to any one of claims 1 to 4, characterized in that as the liquid carrier, an aqueous solution or suspension is added, which contains: 1) 0.1 to 0.5% biophysically derivatized yeast obtained either from one yeast used as starting material and / or from another farm and 0.02 to 0.4% chondriosomes extract, 2) 0.3 to 0.5% cellulose glycolate, fumed silica with more than 99.8% SiO ₂ and a diameter of 7 to 25 nm, carrageenan, tragacanth or similar fillers, 3) 0.01 to 0.3% fermentable mono- and / or disaccharides, 4) preservatives such as parabens and salts such as saline in sufficient concentration, 5) trace elements such as Mn, Co, Zn, Mg, preferably as sulfates or gluconates. 6. The method according to any one of claims 1 to 5, characterized in that a strain from the family of Saccharomycetaceae is used as starting cell material. 7. The method according to any one of claims 1 to 6, characterized in that a dry yeast is treated with an HTS content of 95 to 98% together with 2 to 4 times the amount of a liquid carrier. 8. The method according to claim 1, characterized in that biophysically derivatized yeast preparation with breath-increasing activity, which is measurable in the Warburg vessel of standardized rat liver homogenate and is expressed as the homogenate additionally insufflated by the homogenate oxygen volume, is prepared. 9. The method according to claim 8, characterized in that the respiratory increase factor at 37 ⁰ C is at least 2.0, based on the homogenate under otherwise identical experimental conditions. 10. The method according to claim 8, characterized in that the respiratory increase factor at 37 ⁰ C has a value of 2.0 to 10 and preferably a value of 2.5 and 5.5. 11. A method according to claim 1, characterized in that a biophysically derivatized yeast preparation is prepared, which also contains an L-amino acid oxidase inhibitory factor, measurable in Warburg vessel on a standardized test solution with the enzyme and phenylalanine as L-amino acid and expressed as the ratio Oxygen consumption with and without yeast preparation. 12. The method according to claim 4, characterized in that the L-amino acid oxidase inhibitory factor has a value in the range of about 20 to 80% and preferably a value in the range of 30 to 60%. , 13. The method according to claim 1, characterized in that a yeast preparation is prepared, which has wound-healing activity. 14. The method according to claim 1, characterized in that a yeast preparation is prepared, which additionally shows a RES-activating effect. 15. The method according to claim 1, characterized in that a yeast preparation is prepared, which inhibits the growth of yeasts and microorganisms, such as. B. bifidus, lactobacillus, cocci and soil bacteria positively influenced and thus indirectly promotes the growth of higher plants and algae. 16. The method according to claim 1, characterized in that a yeast preparation is prepared which additionally contains extracted from animal and / or plant cells mitochondrion. 17. Use of the inventively prepared biophysically derivatlsierten preparation, characterized in that it A) for the probiotic activation of dairy products, wherein the derivatized Zellmatarial with selected natural microorganisms growth-stimulating cooperates, is used, or B) is used for the probiotic activation of actinomycetes, in particular for the activation of yeasts. 18. Use according to claim 17 A, characterized in that it is used in yoghurt production. 19. Use according to claim 17A, characterized in that it is used for growth stimulation of Lactobacillus bulgaricus. 20. Use according to claim 17 B, characterized in that it is used for activating yeasts from the following group: Aspergillus niger, Torula utilis, Neurospora crassa, Saccharomyces species and Japanese Ikashiokara yeast. 21 feed and plant growth, characterized in that they contain up to 2Gew .-% of the biophysically derivatized preparation according to one of claims 1 to 16.