DE60216245D1 - Funktionelles oberflächendisplay von polypeptiden - Google Patents

Funktionelles oberflächendisplay von polypeptiden

Info

Publication number
DE60216245D1
DE60216245D1 DE60216245T DE60216245T DE60216245D1 DE 60216245 D1 DE60216245 D1 DE 60216245D1 DE 60216245 T DE60216245 T DE 60216245T DE 60216245 T DE60216245 T DE 60216245T DE 60216245 D1 DE60216245 D1 DE 60216245D1
Authority
DE
Germany
Prior art keywords
polypeptides
surface display
functional surface
display
autotransporter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60216245T
Other languages
English (en)
Other versions
DE60216245T2 (de
Inventor
Joachim Jose
Franck Hannemann
Rita Bernhardt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zyrus Beteiligungs GmbH and Co Patente I KG
Original Assignee
Universitaet des Saarlandes
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universitaet des Saarlandes filed Critical Universitaet des Saarlandes
Application granted granted Critical
Publication of DE60216245D1 publication Critical patent/DE60216245D1/de
Publication of DE60216245T2 publication Critical patent/DE60216245T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Electrochromic Elements, Electrophoresis, Or Variable Reflection Or Absorption Elements (AREA)
  • Materials For Medical Uses (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
DE60216245T 2001-03-02 2002-03-01 Funktionelles oberflächendisplay von polypeptiden Expired - Lifetime DE60216245T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP01105129 2001-03-02
EP01105129 2001-03-02
PCT/EP2002/002246 WO2002070645A2 (en) 2001-03-02 2002-03-01 Functional surface display of polypeptides

Publications (2)

Publication Number Publication Date
DE60216245D1 true DE60216245D1 (de) 2007-01-04
DE60216245T2 DE60216245T2 (de) 2007-06-21

Family

ID=8176655

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60216245T Expired - Lifetime DE60216245T2 (de) 2001-03-02 2002-03-01 Funktionelles oberflächendisplay von polypeptiden

Country Status (7)

Country Link
US (1) US20040259151A1 (de)
EP (1) EP1373571B1 (de)
AT (1) ATE346168T1 (de)
AU (1) AU2002249249A1 (de)
DE (1) DE60216245T2 (de)
DK (1) DK1373571T3 (de)
WO (1) WO2002070645A2 (de)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004065415A2 (en) 2003-01-20 2004-08-05 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. L-amino acid oxidase with cytotoxic activity from aplysia punctata
US7253293B2 (en) * 2004-01-06 2007-08-07 Andrzej Slominski Enzymatic method to produce 7-dehydropregnenolone, vitamin D3-like compounds and derivatives thereof
EP1789094B1 (de) 2004-08-13 2014-12-10 MARSHALL, Barry J. Bakterielles abgabesystem
US8029777B2 (en) 2004-08-13 2011-10-04 Marshall Barry J Helicobacter system and uses thereof
US20080124355A1 (en) 2006-09-22 2008-05-29 David Gordon Bermudes Live bacterial vaccines for viral infection prophylaxis or treatment
CL2008002961A1 (es) * 2007-10-04 2009-05-08 Bio Arch Lab Inc Metodo para convertir un monosacarido u oligosacarido apropiado en un quimico generico que comprende contactar dichos moleculas con un sistema microbiano que comprende uno o mas genes que codifican y expresan una via de biosintesis.
US8241623B1 (en) 2009-02-09 2012-08-14 David Bermudes Protease sensitivity expression system
US9597379B1 (en) 2010-02-09 2017-03-21 David Gordon Bermudes Protease inhibitor combination with therapeutic proteins including antibodies
US8524220B1 (en) 2010-02-09 2013-09-03 David Gordon Bermudes Protease inhibitor: protease sensitivity expression system composition and methods improving the therapeutic activity and specificity of proteins delivered by bacteria
US8771669B1 (en) 2010-02-09 2014-07-08 David Gordon Bermudes Immunization and/or treatment of parasites and infectious agents by live bacteria
DE102010035702A1 (de) * 2010-08-27 2012-03-01 Zyrus Beteiligungsgesellschaft Mbh & Co. Patente I Kg Ganzzell-Biokatalysator
US20140011706A1 (en) * 2010-09-23 2014-01-09 Autodisplay Biotech Gmbh Surface display of polypeptides containing a metal porphyrin or a flavin
CA2811699C (en) * 2010-09-28 2019-07-02 Abera Bioscience Ab Fusion protein for secretory protein expression
CA2827443C (en) 2011-02-15 2021-07-06 Vaxiion Therapeutics, Inc. Therapeutic compositions and methods for antibody and fc-containing targeting molecule-based targeted delivery of bioactive molecules by bacterial minicells
WO2012152923A1 (en) * 2011-05-12 2012-11-15 Autodisplay Biotech Gmbh Whole cell biocatalyst comprising a prenyltransferase
BR112014000947A2 (pt) 2011-07-20 2017-06-13 Evonik Degussa Gmbh oxidação e aminação de álcoois primários
CN103827309A (zh) 2011-08-05 2014-05-28 赢创德固赛有限公司 仲醇的氧化和胺化
EP2602329A1 (de) 2011-12-05 2013-06-12 Evonik Degussa GmbH Biotechnologische Herstellung von 3-Hydroxyisobuttersäure
EP2602328A1 (de) 2011-12-05 2013-06-12 Evonik Industries AG Verfahren zur Oxidation von Alkanen unter Verwendung einer AlkB Alkan 1-Monooxygenase
EP2639308A1 (de) 2012-03-12 2013-09-18 Evonik Industries AG Enzymatische omega-Oxidation und -Aminierung von Fettsäuren
EP2653538A1 (de) 2012-04-20 2013-10-23 Evonik Industries AG NADP-abhängige Alanindehydrogenase
US9593339B1 (en) 2013-02-14 2017-03-14 David Gordon Bermudes Bacteria carrying bacteriophage and protease inhibitors for the treatment of disorders and methods of treatment
EP2970953B1 (de) * 2013-03-13 2020-01-15 Autodisplay Biotech GmbH Verbesserte anzeige von funktionellen proteinen in einem breiten bereich gramnegativer bakterien
US9737592B1 (en) 2014-02-14 2017-08-22 David Gordon Bermudes Topical and orally administered protease inhibitors and bacterial vectors for the treatment of disorders and methods of treatment
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11471497B1 (en) 2019-03-13 2022-10-18 David Gordon Bermudes Copper chelation therapeutics
US10973908B1 (en) 2020-05-14 2021-04-13 David Gordon Bermudes Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine
US12537071B1 (en) 2020-07-22 2026-01-27 David Gordon Bermudes Bacteria having boolean control pathways expressing therapeutic proteins including immunotherapeutic cytotoxins

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4344350C2 (de) * 1993-12-23 1995-09-21 Max Planck Gesellschaft Bakterien zur Herstellung stabiler Fusionsproteine und Verfahren zu deren Nachweis
US6686168B1 (en) * 1999-11-04 2004-02-03 Zymogenetics, Inc. Cell surface display of proteins by recombinant host cells

Also Published As

Publication number Publication date
DE60216245T2 (de) 2007-06-21
EP1373571B1 (de) 2006-11-22
EP1373571A2 (de) 2004-01-02
WO2002070645A2 (en) 2002-09-12
AU2002249249A1 (en) 2002-09-19
DK1373571T3 (da) 2007-04-16
ATE346168T1 (de) 2006-12-15
WO2002070645A3 (en) 2003-07-24
US20040259151A1 (en) 2004-12-23

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Legal Events

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8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: ZYRUS BETEILIGUNGSGESELLSCHAFT MBH & CO. PATEN, DE