DK0493115T3 - Fremgangsmåde til isolering af DNA - Google Patents
Fremgangsmåde til isolering af DNAInfo
- Publication number
- DK0493115T3 DK0493115T3 DK91312018.4T DK91312018T DK0493115T3 DK 0493115 T3 DK0493115 T3 DK 0493115T3 DK 91312018 T DK91312018 T DK 91312018T DK 0493115 T3 DK0493115 T3 DK 0493115T3
- Authority
- DK
- Denmark
- Prior art keywords
- dna
- isolating dna
- deproteination
- xanthate
- homogenizing
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 5
- 241000894006 Bacteria Species 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 abstract 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 abstract 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 1
- 238000002105 Southern blotting Methods 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- JCBJVAJGLKENNC-UHFFFAOYSA-M potassium ethyl xanthate Chemical compound [K+].CCOC([S-])=S JCBJVAJGLKENNC-UHFFFAOYSA-M 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
- 229910052708 sodium Inorganic materials 0.000 abstract 1
- 239000011734 sodium Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/82—Subcellular parts of microorganisms
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US07/634,256 US5204246A (en) | 1990-12-26 | 1990-12-26 | Dna isolation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK0493115T3 true DK0493115T3 (da) | 1995-09-11 |
Family
ID=24543037
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK91312018.4T DK0493115T3 (da) | 1990-12-26 | 1991-12-24 | Fremgangsmåde til isolering af DNA |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US5204246A (da) |
| EP (1) | EP0493115B1 (da) |
| JP (1) | JP2527510B2 (da) |
| AT (1) | ATE125296T1 (da) |
| DE (1) | DE69111406T2 (da) |
| DK (1) | DK0493115T3 (da) |
| ES (1) | ES2077187T3 (da) |
| HU (1) | HU209858B (da) |
| NZ (1) | NZ240503A (da) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5352777A (en) * | 1990-12-26 | 1994-10-04 | Pioneer Hi-Bred International, Inc. | DNA isolation from animal cells |
| GB9425138D0 (en) * | 1994-12-12 | 1995-02-08 | Dynal As | Isolation of nucleic acid |
| WO1999029703A2 (en) * | 1997-12-06 | 1999-06-17 | Dna Research Instruments Limited | Isolation of nucleic acids |
| US6914137B2 (en) * | 1997-12-06 | 2005-07-05 | Dna Research Innovations Limited | Isolation of nucleic acids |
| EP1147226B1 (en) * | 1999-01-27 | 2013-01-23 | Folim G. Halaka | Materials and methods for the purification of polyelectrolytes |
| JP2005538929A (ja) | 2002-01-16 | 2005-12-22 | ダイナル バイオテック エイエスエイ | 単一サンプルからの核酸及びタンパク質の単離方法 |
| WO2004056984A2 (en) * | 2002-12-18 | 2004-07-08 | University Of Geneva | Enzymatic method for the isolation of dna from plant tissue |
| JP5388328B2 (ja) * | 2008-09-29 | 2014-01-15 | 独立行政法人森林総合研究所 | 木材のdnaを分析するための前処理方法 |
| JP5746767B2 (ja) * | 2010-09-15 | 2015-07-08 | ビーエーエスエフ プラント サイエンス カンパニー ゲーエムベーハー | 生物学的サンプルから成分を抽出するための抽出デバイス及び方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| USRE25785E (en) * | 1965-06-01 | Process for preparing a nucleic acid | ||
| US3163638A (en) * | 1960-12-28 | 1964-12-29 | Toyo Boseki | Extraction of ribonucleic acid |
| US3582468A (en) * | 1968-09-27 | 1971-06-01 | Merck & Co Inc | Recovery of double-stranded ribonucleic acid |
| US4830969A (en) * | 1981-08-31 | 1989-05-16 | The Research Foundation Of State University Of New York | Process for the rapid and simple isolation of nucleic acids |
| GB8311905D0 (en) * | 1983-04-29 | 1983-06-02 | Cox R A | Isolation and detection of nucleotide sequence |
| US4843012A (en) * | 1986-09-17 | 1989-06-27 | Genetics Institute, Inc. | Novel composition for nucleic acid purification |
| EP0310913A3 (en) * | 1987-09-30 | 1990-04-25 | Biotechnica Diagnostics Inc | Dna isolation procedure |
| DE68914689T2 (de) * | 1989-05-22 | 1994-10-27 | Genetics Inst | Verbesserte zusammensetzung zur isolierung und anreicherung von nukleinsäure und dadurch verbessertes verfahren. |
-
1990
- 1990-12-26 US US07/634,256 patent/US5204246A/en not_active Expired - Fee Related
-
1991
- 1991-11-07 NZ NZ240503A patent/NZ240503A/en unknown
- 1991-12-20 HU HU914094A patent/HU209858B/hu not_active IP Right Cessation
- 1991-12-24 ES ES91312018T patent/ES2077187T3/es not_active Expired - Lifetime
- 1991-12-24 DE DE69111406T patent/DE69111406T2/de not_active Expired - Fee Related
- 1991-12-24 EP EP91312018A patent/EP0493115B1/en not_active Expired - Lifetime
- 1991-12-24 DK DK91312018.4T patent/DK0493115T3/da active
- 1991-12-24 AT AT91312018T patent/ATE125296T1/de active
- 1991-12-25 JP JP3342874A patent/JP2527510B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP0493115A2 (en) | 1992-07-01 |
| HU209858B (en) | 1994-11-28 |
| HU914094D0 (en) | 1992-03-30 |
| DE69111406D1 (de) | 1995-08-24 |
| JP2527510B2 (ja) | 1996-08-28 |
| NZ240503A (en) | 1994-02-25 |
| ATE125296T1 (de) | 1995-08-15 |
| EP0493115A3 (en) | 1993-02-24 |
| ES2077187T3 (es) | 1995-11-16 |
| DE69111406T2 (de) | 1996-01-04 |
| EP0493115B1 (en) | 1995-07-19 |
| US5204246A (en) | 1993-04-20 |
| HUT62311A (en) | 1993-04-28 |
| JPH0654688A (ja) | 1994-03-01 |
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