DK0595948T3 - Gærstammer med stabil integration af heterologe gener - Google Patents

Gærstammer med stabil integration af heterologe gener

Info

Publication number
DK0595948T3
DK0595948T3 DK92916070.3T DK92916070T DK0595948T3 DK 0595948 T3 DK0595948 T3 DK 0595948T3 DK 92916070 T DK92916070 T DK 92916070T DK 0595948 T3 DK0595948 T3 DK 0595948T3
Authority
DK
Denmark
Prior art keywords
pct
yeast strains
heterologous genes
stable integration
heterologous
Prior art date
Application number
DK92916070.3T
Other languages
English (en)
Inventor
Denis Pompon
Christophe Cullin
Gilles Truan
Philippe Urban
Piotr Slonimski
Original Assignee
Centre Nat Rech Scient
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre Nat Rech Scient filed Critical Centre Nat Rech Scient
Application granted granted Critical
Publication of DK0595948T3 publication Critical patent/DK0595948T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/80—Cytochromes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Distillation Of Fermentation Liquor, Processing Of Alcohols, Vinegar And Beer (AREA)
DK92916070.3T 1991-07-15 1992-07-15 Gærstammer med stabil integration af heterologe gener DK0595948T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9108884A FR2679249B1 (fr) 1991-07-15 1991-07-15 Souches de levure avec integration stable de genes heterologues.

Publications (1)

Publication Number Publication Date
DK0595948T3 true DK0595948T3 (da) 1997-12-15

Family

ID=9415092

Family Applications (1)

Application Number Title Priority Date Filing Date
DK92916070.3T DK0595948T3 (da) 1991-07-15 1992-07-15 Gærstammer med stabil integration af heterologe gener

Country Status (11)

Country Link
US (1) US5635369A (da)
EP (1) EP0595948B1 (da)
AT (1) ATE153381T1 (da)
AU (1) AU663105B2 (da)
CA (1) CA2113477C (da)
DE (1) DE69219894T2 (da)
DK (1) DK0595948T3 (da)
ES (1) ES2103955T3 (da)
FR (1) FR2679249B1 (da)
GR (1) GR3024503T3 (da)
WO (1) WO1993002200A1 (da)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2738840B1 (fr) * 1995-09-19 1997-10-31 Rhone Poulenc Rorer Sa Souches de levures genetiquement modifiees
US5831018A (en) * 1997-02-18 1998-11-03 Incyte Pharmaceuticals, Inc. Human cytochrome B5
AU5060200A (en) * 1999-04-24 2000-11-10 Max-Delbruck-Centrum Fur Molekulare Medizin Nucleic acid sequences from (candida) yeasts which code cytochrome b5 polypeptides
FR2810337B1 (fr) * 2000-06-20 2002-09-13 Univ Clermont Auvergne Micro-organismes actifs dans l'environnement digestif
US6943001B2 (en) * 2001-08-03 2005-09-13 Diversa Corporation Epoxide hydrolases, nucleic acids encoding them and methods for making and using them
CA2456229A1 (en) * 2001-08-03 2003-02-13 Diversa Corporation Epoxide hydrolases, nucleic acids encoding them and methods for making and using them
PL218318B1 (pl) * 2002-05-16 2014-11-28 Bavarian Nordic As Rekombinowany wirus krowianki Ankara (MVA), zawierająca go komórka, szczepionka lub kompozycja farmaceutyczna, sposób lub zestaw do jego otrzymywania, sekwencja DNA zawierająca genom tego wirusa oraz sposób wykrywania tego wirusa lub komórek nim zainfekowanych
WO2010134096A2 (en) 2009-05-19 2010-11-25 Premas Biotech Pvt. Ltd Yeast strain expressing modified human cytochrome p450 reductase and/or cytochrome p450 gene
WO2010134095A2 (en) 2009-05-19 2010-11-25 Premas Biotech Pvt. Ltd Yeast strain expressing modified human cytochrome p450 reductase and/or cytochrome p450 gene
CA2765849A1 (en) 2009-07-02 2011-01-06 Verdezyne, Inc. Biological methods for preparing adipic acid
US8728798B2 (en) 2011-05-03 2014-05-20 Verdezyne, Inc. Biological methods for preparing adipic acid
US8343752B2 (en) 2011-05-03 2013-01-01 Verdezyne, Inc. Biological methods for preparing adipic acid
FR2991340B1 (fr) * 2012-06-01 2016-02-19 Lesaffre & Cie Procede d'obtention de souches de levure ameliorees par modification genetique et croisement

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986007091A1 (en) * 1985-05-21 1986-12-04 Biotechnica International, Inc. Yeast expressing glucoamylase
DK293787A (da) * 1986-06-10 1987-12-11 Transgene Sa Ekspression af en amyloglucosidase
EP0382332A3 (en) * 1989-01-06 1990-11-14 Collaborative Research Inc. Improved supersecreting mutants of saccharomyces cerevisiae
CA2017176A1 (en) * 1989-05-22 1990-11-22 Yoshitomi Pharmaceutical Industries Ltd. Albumin gene-containing plasmid, transformant carrying same, production of such transformant and production of albumin

Also Published As

Publication number Publication date
CA2113477C (fr) 2003-09-23
FR2679249B1 (fr) 1993-11-26
AU663105B2 (en) 1995-09-28
US5635369A (en) 1997-06-03
JPH06508761A (ja) 1994-10-06
AU2387792A (en) 1993-02-23
DE69219894D1 (de) 1997-06-26
EP0595948B1 (fr) 1997-05-21
JP3610410B2 (ja) 2005-01-12
GR3024503T3 (en) 1997-11-28
ATE153381T1 (de) 1997-06-15
FR2679249A1 (fr) 1993-01-22
DE69219894T2 (de) 1998-01-15
ES2103955T3 (es) 1997-10-01
CA2113477A1 (fr) 1993-02-04
EP0595948A1 (fr) 1994-05-11
WO1993002200A1 (fr) 1993-02-04

Similar Documents

Publication Publication Date Title
FR2679249B1 (fr) Souches de levure avec integration stable de genes heterologues.
Sahm et al. Microbial assimilation of methanol: The ethanol-and methanol-oxidizing enzymes of the yeast Candida boidinii
Truan et al. Enhanced in vivo monooxygenase activities of mammalian P450s in engineered yeast cells producing high levels of NADPH-P450 reductase and human cytochrome b5
Søballe et al. Ubiquinone limits oxidative stress in Escherichia coli
Galtier et al. In vitro sulfoxidation of albendazole by ovine liver microsomes: assay and frequency of various xenobiotics
Adak et al. A conserved flavin-shielding residue regulates NO synthase electron transfer and nicotinamide coenzyme specificity
Brutinel et al. Preferential utilization of D-lactate by Shewanella oneidensis
Nielsen et al. Regulation of bacterial methane oxidation: transcription of the soluble methane mono-oxygenase operon of Methylococcus capsulatus (Bath) is repressed by copper ions
Zheng et al. Identification of a meander region proline residue critical for heme binding to cytochrome P450: implications for the catalytic function of human CYP4B1
Sosa et al. Mutants from Chlamydomonas reinhardii affected in their nitrate assimilation capability
Guevara et al. Metabolic engineering of Rhodococcus ruber Chol-4: a cell factory for testosterone production
Römermann et al. Denitrification by Alcaligenes eutrophus is plasmid dependent
Dolin OXIDATION OF REDUCED DIPHOSPHOPYRIDINE NUCLEOTIDE BY CLOSTRIDIUM PERFRINGENS I: Relation of Peroxide to the Over-all Reaction
Vasiliou et al. Mouse dioxin-inducible NAD (P) H: menadione oxidoreductase: NMO1 cDNA sequence and genetic differences in mRNA levels
Härtig et al. The Bacillus subtilis nrdEF genes, encoding a class Ib ribonucleotide reductase, are essential for aerobic and anaerobic growth
Duarte et al. The stimulatory role of human cytochrome b5 in the bioactivation activities of human CYP1A2, 2A6 and 2E1: a new cell expression system to study cytochrome P450 mediated biotransformation
EP0584214A4 (en) MANIPULATION AND EXPRESSION OF GENES USING GENOME ELEMENTS.
FR2693207B1 (fr) Souche de levure permettant la co-expression d'une activité mono-oxygénase de cytochrome P450 hétérologue et d'une NADPH-cytochrome P450-réductase endogène ou hétérologue et son utilisation à des fins de bioconversion.
Hall et al. Structure–function analysis of NADPH: nitrate reductase from Aspergillus nidulans: analysis of altered pyridine nucleotide specificity in vivo
Miszczak-Zaborska et al. The activity of thymidine phosphorylase obtained from human uterine leiomyomas and studied in the presence of pyrimidine derivatives
Loper Cytochrome P450 lanosterol 14α-demethylase (CYP51): Insights from molecular genetic analysis of the ERG11 gene in Saccharomyces cerevisiae
Blatiak et al. Cytochrome P-450 accumulation and loss as controlled by growth phase of Saccharomyces cerevisiae: relationship to oxygen, glucose and ethanol concentrations
van der Oost et al. Reversed electron transfer through the bc1complex enables a cytochrome c oxidase mutant (Δaa3/cbb3) of Paracoccus denitrificans to grow on methylamine
Kato et al. Formation of Tertiary Amine N-Oxide and Its Reduction by Rat Liver Microsomes
김정애 et al. Studies on Acetanilide p-Hydroxylase in Streptomyces spp.