DK0625572T3 - Fremgangsmåde til syntetisering af totallængde cDNA, fremgangsmåde til fremstilling af mellemprodukt dertil og fremgangsmåde til fremstilling af rekombinant vektor indeholdende totallængde CDNA - Google Patents

Fremgangsmåde til syntetisering af totallængde cDNA, fremgangsmåde til fremstilling af mellemprodukt dertil og fremgangsmåde til fremstilling af rekombinant vektor indeholdende totallængde CDNA

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Publication number
DK0625572T3
DK0625572T3 DK93921061T DK93921061T DK0625572T3 DK 0625572 T3 DK0625572 T3 DK 0625572T3 DK 93921061 T DK93921061 T DK 93921061T DK 93921061 T DK93921061 T DK 93921061T DK 0625572 T3 DK0625572 T3 DK 0625572T3
Authority
DK
Denmark
Prior art keywords
total length
length cdna
vector containing
recombinant vector
synthesizing
Prior art date
Application number
DK93921061T
Other languages
English (en)
Inventor
Seishi Kato
Shingo Sekine
Original Assignee
Kanagawa Kagaku Gijutsu Akad
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kanagawa Kagaku Gijutsu Akad filed Critical Kanagawa Kagaku Gijutsu Akad
Application granted granted Critical
Publication of DK0625572T3 publication Critical patent/DK0625572T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK93921061T 1992-09-25 1993-09-22 Fremgangsmåde til syntetisering af totallængde cDNA, fremgangsmåde til fremstilling af mellemprodukt dertil og fremgangsmåde til fremstilling af rekombinant vektor indeholdende totallængde CDNA DK0625572T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP28093292 1992-09-25
JP06758993A JP3337748B2 (ja) 1992-09-25 1993-03-03 完全長ｃＤＮＡの合成方法、その中間体の製造方法及び完全長ｃＤＮＡを含む組換えベクターの製造方法
PCT/JP1993/001359 WO1994008001A1 (fr) 1992-09-25 1993-09-22 PROCEDE POUR SYNTHETISER UN ADNc DE LONGUEUR COMPLETE, PROCEDE POUR PRODUIRE UN INTERMEDIAIRE DE CET ADNc, ET PROCEDE POUR PRODUIRE UN VECTEUR DE RECOMBINAISON CONTENANT CET ADNc

Publications (1)

Publication Number Publication Date
DK0625572T3 true DK0625572T3 (da) 2001-08-13

Family

ID=26408803

Family Applications (1)

Application Number Title Priority Date Filing Date
DK93921061T DK0625572T3 (da) 1992-09-25 1993-09-22 Fremgangsmåde til syntetisering af totallængde cDNA, fremgangsmåde til fremstilling af mellemprodukt dertil og fremgangsmåde til fremstilling af rekombinant vektor indeholdende totallængde CDNA

Country Status (6)

Country Link
US (1) US5597713A (da)
EP (1) EP0625572B1 (da)
JP (1) JP3337748B2 (da)
DE (1) DE69330162T2 (da)
DK (1) DK0625572T3 (da)
WO (1) WO1994008001A1 (da)

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US5766891A (en) 1994-12-19 1998-06-16 Sloan-Kettering Institute For Cancer Research Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase
FR2733762B1 (fr) * 1995-05-02 1997-08-01 Genset Sa Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm et preparation d'arnm et d'adnc complet
FR2733765B1 (fr) * 1995-05-02 1997-07-25 Genset Sa Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm
US5962271A (en) * 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
JP3441899B2 (ja) * 1996-11-01 2003-09-02 理化学研究所 完全長ｃＤＮＡライブラリーの作成方法
CA2263716C (en) * 1997-06-12 2007-09-25 Sloan-Kettering Institute For Cancer Research Covalent joining of dna strands to rna strands catalyzed by vaccinia topoisomerase
ATE338117T1 (de) * 1997-08-01 2006-09-15 Serono Genetics Inst Sa 5' ests für proteine, die nicht gewebespezifisch sind
US6222029B1 (en) 1997-08-01 2001-04-24 Genset 5′ ESTs for secreted proteins expressed in brain
US7393663B2 (en) 1997-08-01 2008-07-01 Serono Genetics Institute S.A. Expressed sequence tags and encoded human proteins
JP2001512005A (ja) * 1997-08-01 2001-08-21 ジェンセット 内胚葉において発現する分泌タンパク質の５’ｅｓｔ
JP2001512012A (ja) * 1997-08-01 2001-08-21 ジェンセット 精巣およびその他の組織で発現する分泌タンパク質の５’ｅｓｔ
EP1367124A1 (en) * 1997-08-01 2003-12-03 Genset 5' ests for secreted proteins expressed in muscle and other mesodermal tissues
JP2001512013A (ja) * 1997-08-01 2001-08-21 ジェンセット 前立腺に発現される分泌タンパク質の５’ｅｓｔ
PT1375514E (pt) * 1997-08-01 2006-10-31 Serono Genetics Inst Sa Ests 5' para proteinas secretadas expressas em varios tecidos
PT1000149E (pt) * 1997-08-01 2007-08-03 Serono Genetics Inst Sa 5' ests para proteínas segregadas identificadas a partir de tecidos do cérebro.
WO1999014364A1 (fr) * 1997-09-18 1999-03-25 Nippon Gene Co., Ltd. PROCEDE DE SYNTHESE D'ADNc A PARTIR D'UN ECHANTILLON D'ARNm
CA2306777A1 (en) * 1997-10-22 1999-04-29 Helix Research Institute Method for screening full-length cdna clones
US6548633B1 (en) 1998-12-22 2003-04-15 Genset, S.A. Complementary DNA's encoding proteins with signal peptides
EP1029045A2 (en) * 1997-11-13 2000-08-23 Genset EXTENDED cDNAs FOR SECRETED PROTEINS
US6312922B1 (en) 1998-02-09 2001-11-06 Genset Complementary DNAs
EP1903111A3 (en) * 1997-11-13 2008-07-02 Serono Genetics Institute S.A. Extended cDNAs for secreted proteins
AU758004B2 (en) * 1997-12-17 2003-03-13 Genset S.A. Extended cDNAs for secreted proteins
JP2002511259A (ja) 1998-04-09 2002-04-16 ジェンセット ５’ｅｓｔおよびコードされるヒトタンパク質
US6783961B1 (en) 1999-02-26 2004-08-31 Genset S.A. Expressed sequence tags and encoded human proteins
US6455280B1 (en) 1998-12-22 2002-09-24 Genset S.A. Methods and compositions for inhibiting neoplastic cell growth
US20040005673A1 (en) 2001-06-29 2004-01-08 Kevin Jarrell System for manipulating nucleic acids
JP2002537762A (ja) * 1999-01-05 2002-11-12 トラスティーズ オブ ボストン ユニバーシティ 改良型核酸クローニング
US6358712B1 (en) 1999-01-05 2002-03-19 Trustee Of Boston University Ordered gene assembly
US20020090612A1 (en) * 1999-01-08 2002-07-11 Jonathan M. Rothberg Method of identifying nucleic acids
EP1586645A3 (en) 1999-02-25 2006-02-22 Ceres Incorporated Sequence-determined DNA fragments and corresponding polypeptides encoded thereby
EP1165768A1 (en) * 1999-03-19 2002-01-02 Invitrogen Corporation METHODS OF OBTAINING FULL-LENGTH NUCLEIC ACID SEQUENCES USING $i(E. COLI) TOPOISOMERASE III AND ITS HOMOLOGS
US7485715B2 (en) 1999-06-18 2009-02-03 Ceres, Inc. Sequence-determined DNA encoding AP2 domain polypeptides
US6395962B1 (en) 1999-06-22 2002-05-28 University Of South Carolina Enhancing expression of a silenced target sequence in plants using plant viral enhancers and amplicons
AU5849600A (en) * 1999-07-08 2001-01-30 Helix Research Institute Method for constructing full-length cdna library
US7479555B2 (en) 1999-07-21 2009-01-20 Ceres, Inc. Polynucleotides having a nucleotide sequence that encodes a polypeptide having MOV34 family activity
US7078501B2 (en) 2000-02-25 2006-07-18 Invitrogen Corporation Topoisomerase linker-mediated amplification methods
US6916632B2 (en) 2000-08-21 2005-07-12 Invitrogen Corporation Methods and reagents for molecular cloning
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
JP4340779B2 (ja) 2000-10-05 2009-10-07 独立行政法人理化学研究所 可変突出部位を含むオリゴヌクレオチドリンカー及び前記リンカーを用いたポリヌクレオチドライブラリーの調製方法
US20060008817A1 (en) 2000-12-08 2006-01-12 Invitrogen Corporation Methods and compositions for generating recombinant nucleic acid molecules
NZ526194A (en) 2000-12-08 2004-10-29 Invitrogen Corp Methods of covalently linking, in one or both strands, two or more double stranded nucleotide sequences using one or more topoisomerases
KR100762261B1 (ko) 2001-04-27 2007-10-04 (주)바이오니아 전장 상보 디옥시리보핵산 제조 방법과 이에 사용되는앵커와 프라이머
US7205128B2 (en) * 2002-08-16 2007-04-17 Agilent Technologies, Inc. Method for synthesis of the second strand of cDNA
EP1616947B1 (en) * 2003-03-28 2012-05-09 Japan as represented by Director General of National Rehabilitation Center for Persons with Disabilities METHOD OF SYNTHESIZING cDNA
FR2861405A1 (fr) * 2003-10-24 2005-04-29 Centre Nat Rech Scient METHODE DE PRODUCTION D'ADNc PLEINE LONGUEUR PAR LIGATURATION D'ADAPTATEUR A EXTREMITE COHESIVE
DE602004027538D1 (de) 2003-12-01 2010-07-15 Life Technologies Corp Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
US20050153333A1 (en) * 2003-12-02 2005-07-14 Sooknanan Roy R. Selective terminal tagging of nucleic acids
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US8124839B2 (en) 2005-06-08 2012-02-28 Ceres, Inc. Identification of terpenoid-biosynthesis related regulatory protein-regulatory region associations
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WO2009135212A2 (en) * 2008-05-02 2009-11-05 Epicentre Technologies Corporation Selective 5' ligation tagging of rna
AU2009242486B2 (en) 2008-05-02 2013-10-10 Cellscript, Inc. RNA polyphosphatase compositions, kits, and uses thereof
US9422602B2 (en) 2012-08-15 2016-08-23 Bio-Rad Laboratories, Inc. Methods and compositions for determining nucleic acid degradation

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Publication number Priority date Publication date Assignee Title
US4661450A (en) 1983-05-03 1987-04-28 Molecular Genetics Research And Development Limited Partnership Molecular cloning of RNA using RNA ligase and synthetic oligonucleotides

Also Published As

Publication number Publication date
EP0625572A1 (en) 1994-11-23
EP0625572B1 (en) 2001-04-18
JP3337748B2 (ja) 2002-10-21
WO1994008001A1 (fr) 1994-04-14
US5597713A (en) 1997-01-28
DE69330162T2 (de) 2001-10-18
JPH06153953A (ja) 1994-06-03
DE69330162D1 (de) 2001-05-23
EP0625572A4 (en) 1997-03-12

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