DK0627954T3 - Fremgangsmåde til elektroeluering af en gel indeholdende adskilte ladede makromolekyler såsom proteiner eller DNA/RNA og et apparat og midler til brug ved fremgangsmåden - Google Patents

Fremgangsmåde til elektroeluering af en gel indeholdende adskilte ladede makromolekyler såsom proteiner eller DNA/RNA og et apparat og midler til brug ved fremgangsmåden

Info

Publication number
DK0627954T3
DK0627954T3 DK92905561.4T DK92905561T DK0627954T3 DK 0627954 T3 DK0627954 T3 DK 0627954T3 DK 92905561 T DK92905561 T DK 92905561T DK 0627954 T3 DK0627954 T3 DK 0627954T3
Authority
DK
Denmark
Prior art keywords
electroeluting
rna
dna
proteins
gel containing
Prior art date
Application number
DK92905561.4T
Other languages
English (en)
Inventor
Peter Andersen
Original Assignee
Peter Andersen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Peter Andersen filed Critical Peter Andersen
Application granted granted Critical
Publication of DK0627954T3 publication Critical patent/DK0627954T3/da

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44739—Collecting the separated zones, e.g. blotting to a membrane or punching of gel spots

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
  • Electrostatic Separation (AREA)
DK92905561.4T 1992-02-25 1992-02-25 Fremgangsmåde til elektroeluering af en gel indeholdende adskilte ladede makromolekyler såsom proteiner eller DNA/RNA og et apparat og midler til brug ved fremgangsmåden DK0627954T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP92905561A EP0627954B1 (en) 1992-02-25 1992-02-25 Process for electroelution of a gel containing separated charged macromolecules, such as proteins or dna/rna, and an apparatus and means for use in the process
CA002130751A CA2130751C (en) 1992-02-25 1992-02-25 Apparatus and process for electroelution of a gel containing charged macromolecules
PCT/DK1992/000058 WO1993016788A1 (en) 1992-02-25 1992-02-25 Process for electroelution of a gel containing separated charged macromolecules, such as proteins or dna/rna, and an apparatus and means for use in the process

Publications (1)

Publication Number Publication Date
DK0627954T3 true DK0627954T3 (da) 1996-08-26

Family

ID=25677447

Family Applications (1)

Application Number Title Priority Date Filing Date
DK92905561.4T DK0627954T3 (da) 1992-02-25 1992-02-25 Fremgangsmåde til elektroeluering af en gel indeholdende adskilte ladede makromolekyler såsom proteiner eller DNA/RNA og et apparat og midler til brug ved fremgangsmåden

Country Status (7)

Country Link
US (1) US5840169A (da)
EP (1) EP0627954B1 (da)
AU (1) AU1324192A (da)
CA (1) CA2130751C (da)
DE (1) DE69210048T2 (da)
DK (1) DK0627954T3 (da)
WO (1) WO1993016788A1 (da)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19939730C2 (de) * 1999-08-21 2003-04-30 Wilhelm Breuer Vorrichtung zur Verwendung bei der Elektroelution von Makromolekülen und ein Verfahren zur Elektroelution von Makromolekülen aus Gelen unter Minimierung des Elutionsvolumens
US6602391B2 (en) 2001-03-05 2003-08-05 Vladimir B. Serikov Apparatus and method for combined capillary separation and blotting of biological macromolecules
US7217513B2 (en) * 2001-07-10 2007-05-15 Massachusetts Institute Of Technology Apparatus and method for isolating a nucleic acid from a sample
WO2003016896A1 (en) * 2001-08-10 2003-02-27 Flesher Robert W A method and device for electrophoresis and blotting
DE10149875A1 (de) * 2001-10-10 2003-07-03 Alpha Technology Ges Fuer Ange Vorrichtung zur Extraktion elektrisch geladener Moleküle
EP1552005A4 (en) * 2002-07-10 2010-12-15 Massachusetts Inst Technology DEVICE AND METHOD FOR ISOLATING A NUCLEIC ACID FROM A SAMPLE
US7250100B2 (en) * 2003-06-17 2007-07-31 Duke University Two dimensional electrophoresis cassette
AU2006261309A1 (en) * 2005-06-18 2006-12-28 Ge Healthcare Bio-Sciences Ab Methods and systems for adding a reagent to an analyte in a gel
EP1917522A1 (en) * 2005-06-18 2008-05-07 GE Healthcare Bio-Sciences AB Method and devices for forming a plurality of wells on a gel
US7528368B2 (en) * 2006-03-23 2009-05-05 Protea Biosciences, Inc. Electrospray ionization process and add-on device with sample injection tip
US7641778B2 (en) * 2006-03-23 2010-01-05 Protea Biosciences, Inc. Gel electroelution and sample separation devices and associated processes
US20080296158A1 (en) * 2007-05-31 2008-12-04 Sharp Kabushiki Kaisha Device for electrophoresis, device for transfer, device for electrophoresis and transfer, chip for electrophoresis and transfer, and method for electrophoresis, method for transfer, and method for electrophoresis and transfer
US20090250345A1 (en) * 2008-04-03 2009-10-08 Protea Biosciences, Inc. Microfluidic electroelution devices & processes
US20090250347A1 (en) * 2008-04-03 2009-10-08 Protea Biosciences, Inc. Microfluidic devices & processes for electrokinetic transport
US8361299B2 (en) * 2008-10-08 2013-01-29 Sage Science, Inc. Multichannel preparative electrophoresis system
EP2347252B1 (en) * 2008-10-08 2014-07-16 Sage Science, Inc. Multichannel preparative electrophoresis system
BR112013032013A2 (pt) 2011-06-16 2016-12-20 British Columbia Cancer Agency método para seleção de tamanho de ácidos nucleidos, aparelho para seleção de tamanho de ácidos nucleidos e cassete para uso na eletroforese de ácidos nucleidos
US9599590B2 (en) 2012-10-12 2017-03-21 Sage Science, Inc. Side-eluting molecular fractionator
JP6612340B2 (ja) 2014-10-15 2019-11-27 セージ サイエンス， インコーポレイテッド 核酸の自動化された加工処理および電気泳動による試料調製のための装置、方法およびシステム
EP3414255B1 (en) 2015-11-20 2021-09-22 Washington University Preparative electrophoretic method for targeted purification of genomic dna fragments
WO2018187779A1 (en) 2017-04-07 2018-10-11 Sage Science, Inc. Systems and methods for detection of genetic structural variation using integrated electrophoretic dna purification
USD1061937S1 (en) 2022-02-25 2025-02-11 Life Technologies Corporation Biomolecule transfer device
JP2025508871A (ja) * 2022-02-25 2025-04-10 ライフ テクノロジーズ コーポレーション 生体分子転写システム及び方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3956099A (en) * 1972-10-30 1976-05-11 Louis Israel Continuous preparative electrophoresis apparatus
US4049534A (en) * 1976-04-12 1977-09-20 E-C Apparatus Corporation Electrophorectic apparatus for elution from preparative gels
US4181594A (en) * 1979-03-27 1980-01-01 University Of Pittsburgh Matrix recovery electrophoresis apparatus
DE3337669C2 (de) * 1983-10-17 1989-09-21 Carl Schleicher & Schuell Gmbh & Co Kg, 3352 Einbeck Gerät zur Elektroelution elektrisch geladener Makromoleküle
GB8331514D0 (en) * 1983-11-25 1984-01-04 Janssen Pharmaceutica Nv Visualization method
DE3417180C1 (de) * 1984-05-09 1985-05-15 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen Vorrichtung zum Eluieren von elektrisch geladenen Makromolekuelen,insbesondere Biopolymeren
US4747918A (en) * 1986-06-18 1988-05-31 William Bellomy Electroelution apparatus and method of using same
DE3829111A1 (de) * 1988-08-27 1990-03-01 Hoechst Ag Verfahren und vorrichtung zum elektrophoretischen trennen, reinigen und anreichern von geladenen oder polarisierbaren makromolekuelen
US4959133A (en) * 1989-01-23 1990-09-25 Eastman Kodak Company Field inversion electroblotting & electroelution

Also Published As

Publication number Publication date
EP0627954A1 (en) 1994-12-14
DE69210048T2 (de) 1996-08-29
EP0627954B1 (en) 1996-04-17
WO1993016788A1 (en) 1993-09-02
DE69210048D1 (de) 1996-05-23
AU1324192A (en) 1993-09-13
CA2130751A1 (en) 1993-08-26
US5840169A (en) 1998-11-24
CA2130751C (en) 2000-01-25

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