DK0672149T3 - Fremgangsmåde til fremstilling/udskilning af et protein med en transformeret skimmelsvamp ved anvendelse af ekspressions/udskilningsregulerende regioner afledt af et Aspergillus-endoxylanase II-gen - Google Patents
Fremgangsmåde til fremstilling/udskilning af et protein med en transformeret skimmelsvamp ved anvendelse af ekspressions/udskilningsregulerende regioner afledt af et Aspergillus-endoxylanase II-genInfo
- Publication number
- DK0672149T3 DK0672149T3 DK93900048T DK93900048T DK0672149T3 DK 0672149 T3 DK0672149 T3 DK 0672149T3 DK 93900048 T DK93900048 T DK 93900048T DK 93900048 T DK93900048 T DK 93900048T DK 0672149 T3 DK0672149 T3 DK 0672149T3
- Authority
- DK
- Denmark
- Prior art keywords
- endoxylanase
- expression
- aspergillus
- gene
- protein
- Prior art date
Links
- 230000028327 secretion Effects 0.000 title abstract 4
- 108010001817 Endo-1,4-beta Xylanases Proteins 0.000 title abstract 3
- 241000228212 Aspergillus Species 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 108090000623 proteins and genes Proteins 0.000 title abstract 2
- 102000004169 proteins and genes Human genes 0.000 title abstract 2
- 230000001105 regulatory effect Effects 0.000 title abstract 2
- 101150062179 II gene Proteins 0.000 title 1
- 238000000605 extraction Methods 0.000 title 1
- 238000002360 preparation method Methods 0.000 title 1
- 241001513093 Aspergillus awamori Species 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 238000012512 characterization method Methods 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2477—Hemicellulases not provided in a preceding group
- C12N9/248—Xylanases
- C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01031—Beta-glucuronidase (3.2.1.31)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01032—Xylan endo-1,3-beta-xylosidase (3.2.1.32), i.e. endo-1-3-beta-xylanase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01136—Glucuronoarabinoxylan endo-1,4-beta-xylanase (3.2.1.136), i.e. feraxanase or feraxan-endoxylanase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL9102051 | 1991-12-09 | ||
| PCT/EP1992/002896 WO1993012237A1 (en) | 1991-12-09 | 1992-12-09 | Process for producing/secreting a protein by a transformed mould using expression/secretion regulating regions derived from an aspergillus endoxylanase ii gene |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK0672149T3 true DK0672149T3 (da) | 2001-11-05 |
Family
ID=19860011
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK93900048T DK0672149T3 (da) | 1991-12-09 | 1992-12-09 | Fremgangsmåde til fremstilling/udskilning af et protein med en transformeret skimmelsvamp ved anvendelse af ekspressions/udskilningsregulerende regioner afledt af et Aspergillus-endoxylanase II-gen |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US5705358A (da) |
| EP (1) | EP0672149B1 (da) |
| JP (1) | JP2866739B2 (da) |
| AT (1) | ATE204022T1 (da) |
| AU (1) | AU664223B2 (da) |
| CA (1) | CA2123416C (da) |
| DE (1) | DE69231995T2 (da) |
| DK (1) | DK0672149T3 (da) |
| ES (1) | ES2159519T3 (da) |
| FI (1) | FI111958B (da) |
| WO (1) | WO1993012237A1 (da) |
| ZA (1) | ZA929539B (da) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5571697A (en) * | 1989-05-05 | 1996-11-05 | Baylor College Of Medicine Texas Medical Center | Expression of processed recombinant lactoferrin and lactoferrin polypeptide fragments from a fusion product in Aspergillus |
| IL108175A0 (en) * | 1992-12-24 | 1994-04-12 | Gist Brocades Nv | Cloning and expression of xylanase B |
| ATE258224T1 (de) * | 1993-03-10 | 2004-02-15 | Novozymes As | Enzyme mit xylanaseaktivität aus aspergillus aculeatus |
| US5811381A (en) * | 1996-10-10 | 1998-09-22 | Mark A. Emalfarb | Cellulase compositions and methods of use |
| US7883872B2 (en) * | 1996-10-10 | 2011-02-08 | Dyadic International (Usa), Inc. | Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose |
| EP0870835A1 (en) * | 1997-04-07 | 1998-10-14 | Unilever N.V. | Agrobacterium mediated transformation of moulds, in particular those belonging to the genus Aspergillus |
| JP4307563B2 (ja) | 1997-04-07 | 2009-08-05 | ユニリーバー・ナームローゼ・ベンノートシャープ | 糸状菌、特にアスペルギルス属に属する糸状菌のアグロバクテリウム媒介性形質転換 |
| US6015703A (en) * | 1998-03-10 | 2000-01-18 | Iogen Corporation | Genetic constructs and genetically modified microbes for enhanced production of beta-glucosidase |
| KR100618495B1 (ko) * | 1998-10-06 | 2006-08-31 | 마크 아론 에말파브 | 섬유상의 진균성 숙주 영역에서의 형질전환 시스템:크리소스포륨속에서 |
| EP1689766B1 (en) * | 2003-11-06 | 2012-04-25 | Danisco US Inc. | Expression in filamentous fungi of protease inhibitors and variants thereof |
| JP5710866B2 (ja) * | 2006-01-23 | 2015-04-30 | ノボザイムス アクティーゼルスカブ | リパーゼ活性を有するポリペプチド及びそれをコードするポリヌクレオチド |
| EP2059590B1 (en) | 2006-07-14 | 2014-12-31 | Novozymes, Inc. | Methods for producing secreted polypeptides having biological activity |
| US9862956B2 (en) | 2006-12-10 | 2018-01-09 | Danisco Us Inc. | Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi |
| EP2505651A3 (en) | 2006-12-10 | 2013-01-09 | Dyadic International, Inc. | Isolated fungus with reduced protease activity |
| US8551751B2 (en) * | 2007-09-07 | 2013-10-08 | Dyadic International, Inc. | BX11 enzymes having xylosidase activity |
| WO2018226900A2 (en) | 2017-06-06 | 2018-12-13 | Zymergen Inc. | A htp genomic engineering platform for improving fungal strains |
| WO2019236848A1 (en) | 2018-06-06 | 2019-12-12 | Zymergen Inc. | Manipulation of genes involved in signal transduction to control fungal morphology during fermentation and production |
| US11479779B2 (en) | 2020-07-31 | 2022-10-25 | Zymergen Inc. | Systems and methods for high-throughput automated strain generation for non-sporulating fungi |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1341226C (en) * | 1988-08-16 | 2001-05-01 | Wim Van Hartingsveldt | Gene replacement as a tool for the construction of aspergillus strains |
| US5171672A (en) * | 1989-08-31 | 1992-12-15 | Allelix Biopharmaceuticals Inc. | Recombinant DNA system for Aspergillus |
| EP0421919A3 (en) * | 1989-09-02 | 1992-04-15 | Ciba-Geigy Ag | Polygalacturonase encoding fungal expression system |
| JP2995770B2 (ja) * | 1989-12-15 | 1999-12-27 | 吉富製薬株式会社 | 異種蛋白質の製造方法 |
| IE68457B1 (en) * | 1990-01-29 | 1996-06-26 | Ciba Geigy Ag | Novel fungal expression system |
| NL9001388A (nl) * | 1990-06-19 | 1992-01-16 | Unilever Nv | Recombinant dna, cel die daarvan afgeleid dna bevat, enzym waarvoor het recombinant dna codeert en toepassingen daarvan. |
| PT98419B (pt) * | 1990-07-24 | 1999-01-29 | Gist Brocades Nv | Processo para a preparacao de sequencias de adn que codificam para xilanases, de construcoes de adn que contem essas sequencias, paraa transformacao de hospedeiros microbianos com essas construcoes, para a preparacao de xilanases por expressao nesses hospedeiros e para a degradacao de xilano por accao dessas xilanases |
-
1992
- 1992-12-09 AU AU31583/93A patent/AU664223B2/en not_active Ceased
- 1992-12-09 US US08/244,686 patent/US5705358A/en not_active Expired - Fee Related
- 1992-12-09 EP EP93900048A patent/EP0672149B1/en not_active Expired - Lifetime
- 1992-12-09 ES ES93900048T patent/ES2159519T3/es not_active Expired - Lifetime
- 1992-12-09 DK DK93900048T patent/DK0672149T3/da active
- 1992-12-09 JP JP5510625A patent/JP2866739B2/ja not_active Expired - Fee Related
- 1992-12-09 WO PCT/EP1992/002896 patent/WO1993012237A1/en not_active Ceased
- 1992-12-09 ZA ZA929539A patent/ZA929539B/xx unknown
- 1992-12-09 AT AT93900048T patent/ATE204022T1/de not_active IP Right Cessation
- 1992-12-09 CA CA002123416A patent/CA2123416C/en not_active Expired - Fee Related
- 1992-12-09 DE DE69231995T patent/DE69231995T2/de not_active Expired - Fee Related
-
1994
- 1994-06-08 FI FI942691A patent/FI111958B/fi not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| EP0672149A1 (en) | 1995-09-20 |
| ES2159519T3 (es) | 2001-10-16 |
| JPH07501453A (ja) | 1995-02-16 |
| DE69231995T2 (de) | 2002-04-04 |
| JP2866739B2 (ja) | 1999-03-08 |
| AU3158393A (en) | 1993-07-19 |
| FI942691L (fi) | 1994-06-08 |
| FI111958B (fi) | 2003-10-15 |
| US5705358A (en) | 1998-01-06 |
| WO1993012237A1 (en) | 1993-06-24 |
| FI942691A0 (fi) | 1994-06-08 |
| EP0672149B1 (en) | 2001-08-08 |
| DE69231995D1 (de) | 2001-09-13 |
| CA2123416C (en) | 2003-05-20 |
| ZA929539B (en) | 1994-06-09 |
| ATE204022T1 (de) | 2001-08-15 |
| CA2123416A1 (en) | 1993-06-24 |
| AU664223B2 (en) | 1995-11-09 |
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