DK0802975T3 - Fremganngsmåde til at reducere RNA koncentration i en blanding af biologisk materiale ved brug af diatoméjord - Google Patents

Fremganngsmåde til at reducere RNA koncentration i en blanding af biologisk materiale ved brug af diatoméjord

Info

Publication number
DK0802975T3
DK0802975T3 DK96903384T DK96903384T DK0802975T3 DK 0802975 T3 DK0802975 T3 DK 0802975T3 DK 96903384 T DK96903384 T DK 96903384T DK 96903384 T DK96903384 T DK 96903384T DK 0802975 T3 DK0802975 T3 DK 0802975T3
Authority
DK
Denmark
Prior art keywords
mixture
concentration
biological material
rna
diatomaceous earth
Prior art date
Application number
DK96903384T
Other languages
English (en)
Inventor
Magda Marquet
Nancy Horn
Gregg Budahazi
Jennifer Meek
Original Assignee
Vical Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vical Inc filed Critical Vical Inc
Application granted granted Critical
Publication of DK0802975T3 publication Critical patent/DK0802975T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/08—Reducing the nucleic acid content

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Saccharide Compounds (AREA)
  • Processing Of Solid Wastes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Treatment Of Sludge (AREA)
DK96903384T 1995-01-13 1996-01-03 Fremganngsmåde til at reducere RNA koncentration i en blanding af biologisk materiale ved brug af diatoméjord DK0802975T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/372,236 US5576196A (en) 1995-01-13 1995-01-13 Process for reducing RNA concentration in a mixture of biological material using diatomaceous earth
PCT/US1996/000247 WO1996021729A1 (en) 1995-01-13 1996-01-03 Process for reducing rna concentration in a mixture of biological material using diatomaceous earth

Publications (1)

Publication Number Publication Date
DK0802975T3 true DK0802975T3 (da) 2003-07-14

Family

ID=23467282

Family Applications (1)

Application Number Title Priority Date Filing Date
DK96903384T DK0802975T3 (da) 1995-01-13 1996-01-03 Fremganngsmåde til at reducere RNA koncentration i en blanding af biologisk materiale ved brug af diatoméjord

Country Status (8)

Country Link
US (1) US5576196A (da)
EP (2) EP0802975B1 (da)
JP (1) JP3808506B2 (da)
AT (2) ATE419341T1 (da)
DE (2) DE69637798D1 (da)
DK (1) DK0802975T3 (da)
ES (1) ES2196140T3 (da)
WO (1) WO1996021729A1 (da)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1444997A (en) 1996-02-06 1997-08-28 Boehringer Mannheim Gmbh Process for preparing purified nucleic acid and the use thereof
FR2746412B1 (fr) * 1996-03-21 1998-06-12 Rhone Poulenc Rorer Sa Purification d'adn plasmidique de qualite pharmaceutique
US7026468B2 (en) 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US7807822B2 (en) 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
US6268492B1 (en) * 1998-08-10 2001-07-31 Chiron Corporation Methods for purifying non-chromosomal nucleic acid molecules from cells
US6617108B1 (en) 1999-07-12 2003-09-09 Technology Licensing Co. Llc Methods and compositions for biotechnical separations using selective precipitation by compaction agents
US20020012990A1 (en) * 1999-12-22 2002-01-31 Lander Russel Jackson Process for the scaleable purification of plasmid DNA
DE10033991A1 (de) * 2000-07-12 2002-01-24 Qiagen Gmbh Verfahren zur Isolierung von Nukleinsäuren
WO2003046146A2 (en) 2001-11-28 2003-06-05 Applera Corporation Compositions and methods of selective nucleic acid isolation
US7314746B2 (en) 2002-09-13 2008-01-01 Valentis, Inc. Apparatus and method for preparative scale purification of nucleic acids
JP4723254B2 (ja) * 2002-12-23 2011-07-13 バイカル インコーポレイテッド プラスミドｄｎａを精製するための方法
US8501402B2 (en) 2003-03-24 2013-08-06 Boehringer Ingelheim Rcv Gmbh & Co Kg Methods and devices for producing biomolecules
JP2004000922A (ja) * 2003-03-26 2004-01-08 Toyobo Co Ltd 核酸またはタンパク質抽出用シリカ粒子組成物
AU2006211216B2 (en) * 2005-01-31 2011-02-03 Merck Sharp & Dohme Llc Purification process for plasmid DNA
EP2088196A1 (en) 2008-02-08 2009-08-12 Boehringer Ingelheim RCV GmbH & Co KG Methods and devices for producing biomolecules
WO2010075116A2 (en) * 2008-12-15 2010-07-01 Life Technologies Corporation Nucleic acid purification apparatus and method
US9024008B2 (en) * 2010-07-07 2015-05-05 Diagon Ltd. Procedure for the specific isolation of total DNA content of bacterial germs and a kit for this purpose
GB201614050D0 (en) * 2016-08-17 2016-09-28 Glaxosmithkline Ip Dev Ltd Method for purifying viral vectors
WO2019004298A1 (ja) * 2017-06-27 2019-01-03 ダイキン工業株式会社 含フッ素ポリマー製造工程から生じる水性流体を処理する方法およびシステム
EP3473336B1 (en) 2017-10-17 2020-12-02 Sartorius Stedim Biotech GmbH Diatomaceous earth composition with a low endotoxin content
AU2020275885B2 (en) * 2019-05-15 2026-02-05 Translate Bio, Inc. Methods for purification of messenger RNA
JP7511829B2 (ja) * 2020-02-28 2024-07-08 国立大学法人 宮崎大学 核酸吸着材
EP4549013A1 (en) * 2023-11-02 2025-05-07 Curiosity Diagnostics sp. z o.o Stable particle compositions
WO2025252480A1 (en) 2024-06-07 2025-12-11 F. Hoffmann-La Roche Ag Method for purifying plasmid dna

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3639949A1 (de) * 1986-11-22 1988-06-09 Diagen Inst Molekularbio Verfahren zur trennung von langkettigen nukleinsaeuren
US5075430A (en) * 1988-12-12 1991-12-24 Bio-Rad Laboratories, Inc. Process for the purification of DNA on diatomaceous earth
NL8900725A (nl) * 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
US5234809A (en) * 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
CA2067712A1 (en) * 1991-05-03 1992-11-04 Daniel L. Woodard Filtration purification of dna
US5155018A (en) * 1991-07-10 1992-10-13 Hahnemann University Process and kit for isolating and purifying RNA from biological sources
DE69324716T2 (de) * 1992-02-13 1999-09-09 Becton Dickinson And Co. Celithydrat und Reinigung von DNS
JP3761573B2 (ja) * 1994-06-14 2006-03-29 インヴィテーク ゲーエムベーハー 極度に少量でかつ非常に強く汚染された非常に種々の出発物質から核酸を単離および精製する一般的方法

Also Published As

Publication number Publication date
ATE235547T1 (de) 2003-04-15
DE69637798D1 (de) 2009-02-12
EP0802975A1 (en) 1997-10-29
EP0802975B1 (en) 2003-03-26
JPH11502403A (ja) 1999-03-02
DE69626957T2 (de) 2004-03-04
US5576196A (en) 1996-11-19
JP3808506B2 (ja) 2006-08-16
ATE419341T1 (de) 2009-01-15
EP1291421A1 (en) 2003-03-12
WO1996021729A1 (en) 1996-07-18
DE69626957D1 (de) 2003-04-30
EP1291421B1 (en) 2008-12-31
ES2196140T3 (es) 2003-12-16

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