DK1123386T3 - Fremgangsmåde til fremstilling af chirale carboxylsyrer af nitriler ved hjælp af en nitrilase eller mikroorganismer, som indeholder et gen for nitrilasen - Google Patents

Fremgangsmåde til fremstilling af chirale carboxylsyrer af nitriler ved hjælp af en nitrilase eller mikroorganismer, som indeholder et gen for nitrilasen

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Publication number
DK1123386T3
DK1123386T3 DK99952558T DK99952558T DK1123386T3 DK 1123386 T3 DK1123386 T3 DK 1123386T3 DK 99952558 T DK99952558 T DK 99952558T DK 99952558 T DK99952558 T DK 99952558T DK 1123386 T3 DK1123386 T3 DK 1123386T3
Authority
DK
Denmark
Prior art keywords
nucleic acid
nitrilase
acid sequences
carboxylic acids
nitriles
Prior art date
Application number
DK99952558T
Other languages
English (en)
Inventor
Ralf Mattes
Bernhard Hauer
Marion Ress-Loeschke
Thomas Friedrich
Dirk Engels
Original Assignee
Basf Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Ag filed Critical Basf Ag
Application granted granted Critical
Publication of DK1123386T3 publication Critical patent/DK1123386T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C12P41/006—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by reactions involving C-N bonds, e.g. nitriles, amides, hydantoins, carbamates, lactames, transamination reactions, or keto group formation from racemic mixtures
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
DK99952558T 1998-10-19 1999-10-13 Fremgangsmåde til fremstilling af chirale carboxylsyrer af nitriler ved hjælp af en nitrilase eller mikroorganismer, som indeholder et gen for nitrilasen DK1123386T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19848129A DE19848129A1 (de) 1998-10-19 1998-10-19 Verfahren zur Herstellung chiraler Carbonsäuren aus Nitrilen mit Hilfe einer Nitrilase oder Mikroorganismen, die ein Gen für die Nitrilase enthalten
PCT/EP1999/007679 WO2000023577A1 (de) 1998-10-19 1999-10-13 Verfahren zur herstellung chiraler carbonsäuren aus nitrilen mit hilfe einer nitrilase oder mikroorganismen, die ein gen für die nitrilase enthalten

Publications (1)

Publication Number Publication Date
DK1123386T3 true DK1123386T3 (da) 2007-05-07

Family

ID=7884933

Family Applications (1)

Application Number Title Priority Date Filing Date
DK99952558T DK1123386T3 (da) 1998-10-19 1999-10-13 Fremgangsmåde til fremstilling af chirale carboxylsyrer af nitriler ved hjælp af en nitrilase eller mikroorganismer, som indeholder et gen for nitrilasen

Country Status (21)

Country Link
US (1) US6869783B1 (da)
EP (1) EP1123386B1 (da)
JP (1) JP4489298B2 (da)
KR (1) KR100715744B1 (da)
CN (1) CN100422319C (da)
AT (1) ATE349517T1 (da)
AU (1) AU765480B2 (da)
BR (1) BR9914629A (da)
CA (1) CA2347521C (da)
CZ (1) CZ302494B6 (da)
DE (2) DE19848129A1 (da)
DK (1) DK1123386T3 (da)
EE (1) EE05008B1 (da)
ES (1) ES2280125T3 (da)
HU (1) HU228092B1 (da)
ID (1) ID29136A (da)
IL (2) IL142397A0 (da)
NO (1) NO327857B1 (da)
PT (1) PT1123386E (da)
WO (1) WO2000023577A1 (da)
ZA (1) ZA200104066B (da)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19848129A1 (de) * 1998-10-19 2000-04-20 Basf Ag Verfahren zur Herstellung chiraler Carbonsäuren aus Nitrilen mit Hilfe einer Nitrilase oder Mikroorganismen, die ein Gen für die Nitrilase enthalten
US20040002147A1 (en) * 1999-12-29 2004-01-01 Desantis Grace Nitrilases
DE10010149A1 (de) * 2000-03-03 2001-09-06 Basf Ag Nitrilase aus Rhodococcus rhodochrous NCIMB 11216
US6562603B2 (en) 2000-08-04 2003-05-13 E. I. Du Pont De Nemours And Company 3-hydroxycarboxylic acid production and use in branched polymers
US6455730B1 (en) 2000-08-04 2002-09-24 E. I. Du Pont De Nemours & Company Preparation of dicarboxylic acid monoesters from cyanocarboxylic acid esters
WO2002029079A2 (en) * 2000-10-04 2002-04-11 Maxygen, Inc. Enantioselective production of amino carboxylic acids
FR2822460B1 (fr) * 2001-03-26 2005-04-29 Rhodia Chimie Sa Procede de preparation enantioselectif d'acides carboxyliques optiquement actifs par hydrolyse enzymatique de nitriles
EP2039762A3 (en) 2001-06-21 2009-07-29 Verenium Corporation Nitralases
ITMI20011826A1 (it) * 2001-08-30 2003-03-02 Istituto Biochimico Italiano Microorganismo dotato di attivita' nitrile-idratasica/amidasica enantioselettiva e regioselettiva
ES2288630T3 (es) 2002-12-02 2008-01-16 Basf Aktiengesellschaft Sistema de expresion, que puede ser inducido por medio de la l-ramnosa.
US8815569B2 (en) 2003-01-08 2014-08-26 Basf Se Methods for preserving and/or storing cells having a nitrilase or nitrile hydratase activity
AT412092B (de) * 2003-02-27 2004-09-27 Dsm Fine Chem Austria Gmbh Verfahren zur herstellung chiraler alpha-hydroxycarbonsäuren durch enzymatische hydrolyse von chiralen cyanhydrinen
EP1599584B1 (en) * 2003-02-27 2009-07-01 Basf Se Modified nitrilases and their use in methods for the production of carboxylic acids
CN102796770B (zh) * 2004-12-22 2014-06-04 纳幕尔杜邦公司 乙醇酸的酶促生产
US20100267100A1 (en) * 2007-03-21 2010-10-21 Ling Hua Enzymatic synthesis of optically pure beta-hydroxy carboxylic acids and their derivatives
US20090004720A1 (en) * 2007-06-26 2009-01-01 Ling Hua Smart Biocatalysts For Organic Synthesis
EP2338987A1 (en) * 2009-11-16 2011-06-29 ZYRUS Beteiligungsgesellschaft mbH & Co. Patente I KG Whole cell biocatalyst
JP2013162746A (ja) * 2010-05-24 2013-08-22 Mitsui Chemicals Inc アミド化合物の製造方法
WO2012069434A1 (de) 2010-11-24 2012-05-31 Basf Se Verfahren zur herstellung n-heterozyklischer optisch aktiver alkohole
KR101223665B1 (ko) * 2011-01-26 2013-01-17 재단법인 경북해양바이오산업연구원 나이트릴레이즈 ｒｍｎ２을 이용한 카르복실산의 제조방법
KR101223664B1 (ko) * 2011-01-26 2013-01-17 재단법인 경북해양바이오산업연구원 나이트릴레이즈 ｒｍｎ１을 이용한 카르복실산의 제조방법
KR101223663B1 (ko) * 2011-01-26 2013-01-21 재단법인 경북해양바이오산업연구원 나이트릴레이즈 ｖｍｎ１을 이용한 카르복실산의 제조방법
KR101223666B1 (ko) * 2011-01-26 2013-01-17 재단법인 경북해양바이오산업연구원 나이트릴레이즈 ｏｒｎ을 이용한 카르복실산의 제조방법
JP2023526068A (ja) 2020-05-15 2023-06-20 ビーエーエスエフ ソシエタス・ヨーロピア イソプレノイド生成の改善方法
JP2024545115A (ja) 2021-12-10 2024-12-05 ビーエーエスエフ ソシエタス・ヨーロピア アルキルホスフィナートの除草活性
EP4444731A1 (en) 2021-12-10 2024-10-16 Basf Se Enzymatic decarbamoylation of glufosinate derivatives
IL313405A (en) 2021-12-10 2024-08-01 Basf Se Synthesis of glufosinate using a hydantoinase-based process
EP4634395A1 (en) 2022-12-16 2025-10-22 Basf Se New ethylenediamine-n,n'-disuccinic acid (edds) synthases
CN121335908A (zh) 2023-06-13 2026-01-13 巴斯夫欧洲公司 使用基于酰胺酶的工艺的草铵膦合成
WO2025104224A1 (en) 2023-11-17 2025-05-22 Basf Se Edds synthase variants
CN122422291A (zh) 2023-11-17 2026-07-17 巴斯夫欧洲公司 S,s-乙二胺-n,n’-二琥珀酸的结晶形式、相应制备方法及用途
EP4556568A1 (en) 2023-11-17 2025-05-21 Basf Se Biocatalytical methods for producing ethylenediamine-n,n'-disuccinic acid
WO2025219238A1 (en) 2024-04-15 2025-10-23 Basf Se Process for producing l-glufosinate from a diol or diol derivative
WO2025219237A1 (en) 2024-04-15 2025-10-23 Basf Se A process for the preparation of 2-oxo-4-(hydroxy(methyl)phosphinoyl)butyric acid

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0332379B1 (en) 1988-03-08 1996-08-14 Japan Energy Corporation Production process of L-alpha-amino acids
US5283193A (en) 1988-06-27 1994-02-01 Asahi Kasei Kogyo K.K. Process for producing optically active α-substituted organic acid and microorganism and enzyme used therefor
JP2623345B2 (ja) 1988-06-27 1997-06-25 旭化成工業株式会社 光学活性なα―置換有機酸の製造方法
JPH0219577A (ja) 1988-07-04 1990-01-23 Nippon Saafuakutanto Kogyo Kk 反応染料用均染剤組成物
DE69131217T2 (de) * 1990-03-30 1999-09-23 Mitsubishi Rayon Co., Ltd. Verfahren zur Herstellung von R(-)Mandelsäure und deren Derivaten
JP2974737B2 (ja) 1990-08-16 1999-11-10 三菱レイヨン株式会社 光学活性乳酸の製造法
ATE134706T1 (de) 1990-09-20 1996-03-15 Du Pont Verfahren zur herstellung von 2- alkansäureenantiomeren
JP2676568B2 (ja) 1991-06-26 1997-11-17 日東化学工業株式会社 Ｒ（−）−マンデル酸およびその誘導体の製造法
JP3093056B2 (ja) * 1992-11-17 2000-10-03 三菱レイヨン株式会社 ニトリラーゼ活性を有するポリペプチドをコードする遺伝子ｄｎａを含有する形質転換体による有機酸の製造法
JP3218133B2 (ja) 1993-02-03 2001-10-15 三菱レイヨン株式会社 フェニル基を有する光学活性α−ヒドロキシカルボン酸の製造法
JP2720140B2 (ja) 1993-02-03 1998-02-25 日東化学工業株式会社 フェニル基を有する光学活性α−ヒドロキシカルボン酸の製造法
JP3354688B2 (ja) 1994-01-28 2002-12-09 三菱レイヨン株式会社 微生物によるα−ヒドロキシ酸またはα−ヒドロキシアミドの製造法
JP3154633B2 (ja) 1994-12-28 2001-04-09 三菱レイヨン株式会社 ニトリラーゼ遺伝子発現のための調節因子およびその遺伝子
WO1997032030A1 (en) 1996-02-29 1997-09-04 Nippon Soda Co., Ltd. PROCESS FOR PREPARING α-HYDROXY ACIDS USING MICROORGANISM AND NOVEL MICROORGANISM
DE19848129A1 (de) * 1998-10-19 2000-04-20 Basf Ag Verfahren zur Herstellung chiraler Carbonsäuren aus Nitrilen mit Hilfe einer Nitrilase oder Mikroorganismen, die ein Gen für die Nitrilase enthalten

Also Published As

Publication number Publication date
KR100715744B1 (ko) 2007-05-08
ZA200104066B (en) 2002-07-01
HUP0104802A2 (hu) 2002-04-29
DE19848129A1 (de) 2000-04-20
ATE349517T1 (de) 2007-01-15
IL142397A0 (en) 2002-03-10
CA2347521A1 (en) 2000-04-27
PT1123386E (pt) 2007-03-30
CN1331743A (zh) 2002-01-16
EP1123386A1 (de) 2001-08-16
EE200100232A (et) 2002-08-15
AU6470899A (en) 2000-05-08
CA2347521C (en) 2010-02-23
NO327857B1 (no) 2009-10-05
AU765480B2 (en) 2003-09-18
ES2280125T3 (es) 2007-09-01
CZ20011382A3 (cs) 2002-03-13
HUP0104802A3 (en) 2005-10-28
EE05008B1 (et) 2008-04-15
US6869783B1 (en) 2005-03-22
KR20010085938A (ko) 2001-09-07
CZ302494B6 (cs) 2011-06-15
NO20011912L (no) 2001-04-18
HU228092B1 (en) 2012-10-29
WO2000023577A1 (de) 2000-04-27
JP2002527106A (ja) 2002-08-27
DE59914102D1 (de) 2007-02-08
IL142397A (en) 2008-03-20
ID29136A (id) 2001-08-02
CN100422319C (zh) 2008-10-01
BR9914629A (pt) 2001-06-26
NO20011912D0 (no) 2001-04-18
JP4489298B2 (ja) 2010-06-23
EP1123386B1 (de) 2006-12-27

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