DK1337547T3 - Nuklease-baseret fremgangsmåde til detektion og kvantificering af oligonukleotider - Google Patents

Nuklease-baseret fremgangsmåde til detektion og kvantificering af oligonukleotider

Info

Publication number
DK1337547T3
DK1337547T3 DK01994359.6T DK01994359T DK1337547T3 DK 1337547 T3 DK1337547 T3 DK 1337547T3 DK 01994359 T DK01994359 T DK 01994359T DK 1337547 T3 DK1337547 T3 DK 1337547T3
Authority
DK
Denmark
Prior art keywords
oigonucleotides
nuclease
quantification
detection
based procedure
Prior art date
Application number
DK01994359.6T
Other languages
English (en)
Inventor
Zhengrong Yu
Brenda F Baker
John Wu
Original Assignee
Isis Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Isis Pharmaceuticals Inc filed Critical Isis Pharmaceuticals Inc
Application granted granted Critical
Publication of DK1337547T3 publication Critical patent/DK1337547T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
DK01994359.6T 2000-11-03 2001-10-23 Nuklease-baseret fremgangsmåde til detektion og kvantificering af oligonukleotider DK1337547T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US70558700A 2000-11-03 2000-11-03
PCT/US2001/049702 WO2002059137A1 (en) 2000-11-03 2001-10-23 Nuclease-based method for detecting and quantitating oligonucleotides

Publications (1)

Publication Number Publication Date
DK1337547T3 true DK1337547T3 (da) 2014-06-10

Family

ID=24834124

Family Applications (1)

Application Number Title Priority Date Filing Date
DK01994359.6T DK1337547T3 (da) 2000-11-03 2001-10-23 Nuklease-baseret fremgangsmåde til detektion og kvantificering af oligonukleotider

Country Status (4)

Country Link
US (1) US8163477B2 (da)
EP (1) EP1337547B1 (da)
DK (1) DK1337547T3 (da)
WO (1) WO2002059137A1 (da)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7179796B2 (en) 2000-01-18 2007-02-20 Isis Pharmaceuticals, Inc. Antisense modulation of PTP1B expression
US6261840B1 (en) * 2000-01-18 2001-07-17 Isis Pharmaceuticals, Inc. Antisense modulation of PTP1B expression
US20020055479A1 (en) * 2000-01-18 2002-05-09 Cowsert Lex M. Antisense modulation of PTP1B expression
KR100436554B1 (ko) * 2001-04-17 2004-06-18 삼성전자주식회사 혼성화된 핵산의 검출감도를 증가시키는 방법
US20060089325A1 (en) * 2004-10-13 2006-04-27 Sanjay Bhanot Antisense modulation of PTP1B expression
EP2061901A2 (en) * 2006-10-31 2009-05-27 Noxxon Pharma AG Methods for detection of a single- or double-stranded nucleic acid molecule
KR100993349B1 (ko) 2007-02-23 2010-11-10 주식회사 파나진 지지체에 고정된 ｐｎａ 프로브와 표적핵산의 혼성화 효율또는 특이도를 증가시키기 위한 방법, 조성물 및 키트
US8389488B2 (en) 2007-11-05 2013-03-05 Isis Pharmaceuticals, Inc. Antidotes to antisense compounds
CN105886506A (zh) 2011-04-13 2016-08-24 Isis制药公司 Ptp1b 表达的反义调节
AU2011372509B2 (en) * 2011-07-01 2017-02-09 Htg Molecular Diagnostics, Inc. Methods of detecting gene fusions
WO2014201306A1 (en) * 2013-06-12 2014-12-18 Oncoimmunin, Inc. Systemic in vivo delivery of oligonucleotides
US10752940B2 (en) 2013-11-08 2020-08-25 Ionis Pharmaceuticals, Inc. Compounds and methods for detecting oligonucleotides
CN104569394A (zh) * 2014-12-24 2015-04-29 深圳市老年医学研究所 一种多重miRNA肿瘤标志物检测方法及其应用
EP3256601B1 (en) * 2015-02-11 2021-04-07 Biogen MA Inc. Methods for detecting therapeutic nucleic acids
CN108414760A (zh) * 2018-03-09 2018-08-17 深圳市诺亚基因科技有限公司 一种利用rna核酸适配体及纳米金检测血清中的茶碱的方法
EP4051791A4 (en) * 2019-10-30 2023-12-06 Takeda Pharmaceutical Company Limited METHOD FOR DETECTING OLIGONUCLEOTIDES

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69132765T2 (de) * 1990-12-24 2002-02-21 Enzo Diagnostics, Inc. Verfahren zum Nachweis eines Zielpolynukleotids in einer Probe unter Verwendung eines Reagenz, das den Hintergrund verringert, und eine dieses Reagenz enthaltende Zusammensetzung und Kit.
EP0667918B1 (en) * 1991-11-14 2000-02-16 Digene Diagnostics, Inc. Non-radioactive hybridization assay and kit
US6136531A (en) * 1995-05-08 2000-10-24 Roche Diagnostics Gmbh Method of quantitatively detecting nucleic acids
US5945290A (en) * 1998-09-18 1999-08-31 Isis Pharmaceuticals, Inc. Antisense modulation of RhoA expression
US6403309B1 (en) * 1999-03-19 2002-06-11 Valigen (Us), Inc. Methods for detection of nucleic acid polymorphisms using peptide-labeled oligonucleotides and antibody arrays
US6573048B1 (en) * 2000-04-18 2003-06-03 Naxcor Degradable nucleic acid probes and nucleic acid detection methods
US6900013B1 (en) * 2000-08-25 2005-05-31 Aviva Biosciences Corporation Methods and compositions for identifying nucleic acid molecules using nucleolytic activities and hybridization

Also Published As

Publication number Publication date
US8163477B2 (en) 2012-04-24
EP1337547B1 (en) 2014-04-30
EP1337547A1 (en) 2003-08-27
EP1337547A4 (en) 2004-05-06
US20040005618A1 (en) 2004-01-08
WO2002059137A1 (en) 2002-08-01

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