DK1673625T3 - Generering af stabiliserede proteiner ved kombinatorisk konsensus-mutagenese - Google Patents

Generering af stabiliserede proteiner ved kombinatorisk konsensus-mutagenese

Info

Publication number
DK1673625T3
DK1673625T3 DK04788755.9T DK04788755T DK1673625T3 DK 1673625 T3 DK1673625 T3 DK 1673625T3 DK 04788755 T DK04788755 T DK 04788755T DK 1673625 T3 DK1673625 T3 DK 1673625T3
Authority
DK
Denmark
Prior art keywords
generation
stabilized proteins
consensus mutagenesis
combinatorial consensus
stabilized
Prior art date
Application number
DK04788755.9T
Other languages
English (en)
Inventor
Wolfgang Aehle
Sandra W Ramer
Volker Schellenberger
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Application granted granted Critical
Publication of DK1673625T3 publication Critical patent/DK1673625T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/02—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amides (3.5.2)
    • C12Y305/02006—Beta-lactamase (3.5.2.6)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DK04788755.9T 2003-10-16 2004-09-15 Generering af stabiliserede proteiner ved kombinatorisk konsensus-mutagenese DK1673625T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/688,255 US20050084868A1 (en) 2003-10-16 2003-10-16 Generation of stabilized proteins by combinatorial consensus mutagenesis
PCT/US2004/030085 WO2005040344A2 (en) 2003-10-16 2004-09-15 Generation of stabilized proteins by combinatorial consensus mutagenesis

Publications (1)

Publication Number Publication Date
DK1673625T3 true DK1673625T3 (da) 2010-04-19

Family

ID=34521130

Family Applications (1)

Application Number Title Priority Date Filing Date
DK04788755.9T DK1673625T3 (da) 2003-10-16 2004-09-15 Generering af stabiliserede proteiner ved kombinatorisk konsensus-mutagenese

Country Status (6)

Country Link
US (1) US20050084868A1 (da)
EP (1) EP1673625B1 (da)
AT (1) ATE451616T1 (da)
DE (1) DE602004024557D1 (da)
DK (1) DK1673625T3 (da)
WO (1) WO2005040344A2 (da)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006116076A2 (en) 2005-04-28 2006-11-02 Genencor International, Inc. Tab molecules
AR061365A1 (es) * 2006-06-13 2008-08-20 Athenix Corp Metodos para generar diversidad genetica mediante mutagenesis con permutaciones
AU2007347119B2 (en) 2006-11-29 2012-02-02 BASF Agricultural Solutions Seed US LLC Improved GRG23 EPSP synthases: compositions and methods of use
EP2476754A1 (en) 2011-01-14 2012-07-18 Bundesrepublik Deutschland, Letztvertreten Durch Den Präsidenten Des Paul-Ehrlich-Instituts Methods for the identification and repair of amino acid residues destabilizing single-chain variable fragments (scFv)
WO2022270481A1 (ja) * 2021-06-22 2022-12-29 花王株式会社 嗅覚受容体の発現方法及び応答測定方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5965408A (en) * 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US6713279B1 (en) * 1995-12-07 2004-03-30 Diversa Corporation Non-stochastic generation of genetic vaccines and enzymes
US6368861B1 (en) * 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6365410B1 (en) * 1999-05-19 2002-04-02 Genencor International, Inc. Directed evolution of microorganisms
US6582914B1 (en) * 2000-10-26 2003-06-24 Genencor International, Inc. Method for generating a library of oligonucleotides comprising a controlled distribution of mutations
EP1415270B1 (en) * 2001-07-31 2016-08-03 Quotainne Enterprises LLC Data carrier comprising an array of contacts

Also Published As

Publication number Publication date
EP1673625B1 (en) 2009-12-09
EP1673625A4 (en) 2007-07-11
DE602004024557D1 (de) 2010-01-21
US20050084868A1 (en) 2005-04-21
ATE451616T1 (de) 2009-12-15
WO2005040344A2 (en) 2005-05-06
EP1673625A2 (en) 2006-06-28
WO2005040344A3 (en) 2005-08-11

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