DK1694846T3 - Celle med forbedret sekretion formidlet af MrgA protein eller en homolog - Google Patents
Celle med forbedret sekretion formidlet af MrgA protein eller en homolog Download PDFInfo
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- DK1694846T3 DK1694846T3 DK04803012.6T DK04803012T DK1694846T3 DK 1694846 T3 DK1694846 T3 DK 1694846T3 DK 04803012 T DK04803012 T DK 04803012T DK 1694846 T3 DK1694846 T3 DK 1694846T3
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- bacillus
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- acid sequence
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Claims (10)
1 En rekombinant Bacillus-ceWe til fremstilling af et heterologt exoprotein af interesse, hvor cellen omfatter et nukleinsyrekonstrukt, der koder for det heterologe exoprotein af interesse og: a) en heterolog promoter, der er operabelt forbundet med i det mindste et gen, der koder for MrgA-protein med en aminosyresekvens, som er i det mindste 70% identisk med aminosyresekvensen vist i SEQ ID NO:2; eller b) i det mindste et heterologt gen, der koder for MrgA-protein med en aminosyresekvens, som er i det mindste 70% identisk med aminosyresekvensen vist i SEQ ID NO:2.
2. Celle ifølge krav 1, som er valgt fra gruppen bestående af Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus brevis, Bacillus circulans, Bacillus coagulans, Bacillus lautus, Bacillus lentus, Bacillus licheniformis, Bacillus stearothermophilus, Bacillus subtilis, og Bacillus thuringiensis.
3. Celle ifølge ethvert af kravene 1 - 2, hvor det heterologe exoprotein er en protease, en lipase, en cutinase, en amylase, en galactosidase, en pullulanase, en cellulase, en glucoseisomerase, en proteindisulfidisomerase, en CGT'ase (cyclodextrinluconotransferase), en phytase, en glucoseoxidase, en glucosyltransferase, en laktase, en bilirubinoxidase, en xylanase, et antigent microbielt eller protozoan protein, et bakterielt proteintoxin, et mikrobielt overfladeprotein eller et viralt protein.
4. Celle ifølge ethvert af kravene 1 - 3, hvor MrgA-proteinet omfatter aminosyresekvensen vist i SEQ ID NO:2.
5. Celle ifølge ethvert af kravene 1 - 4, som omfatter i det mindste et exogent gen, der koder for MrgA-proteinet omfattende en aminosyresekvens, som er i det mindste 70% identisk med aminosyresekvensen vist i SEQ ID NO:2.
6. Celle ifølge ethvert af kravene 1 - 5, som omfatter i det mindste et exogent gen, der koder for MrgA-protein omfattende aminosyresekvensen som vist i SEQ ID NO:2.
7. Celle ifølge ethvert af kravene 1 - 6, som omfatter i det mindste et exogent gen, der koder for MrgA omfattende en polynukleotidsekvens, som er i det mindste 70% identisk med sekvensen vist i SEQ ID NO:1.
8. Celle ifølge ethvert af kravene 1 - 7, hvor i det mindste et exogent gen, der koder for et MrgA-protein med en aminosyresekvens, som er i det mindste 70% identisk med aminosyresekvensen vist i SEQ ID NO:2, er integreret i cellens genom.
9. Celle ifølge ethvert af kravene 1 - 7, hvor i det mindste et exogent gen, der koder for et MrgA-protein med en aminosyresekvens, som er i det mindste 70% identisk med aminosyresekvensen vist i SEQ ID NO:2, er til stede på et extrakromosonalt konstrukt, fortrinsvis et plasmid.
10. Fremgangsmåde til fremstilling af et heterologt exoprotein af interesse, omfattende trinene: a) dyrkning af cellen ifølge ethvert af kravene 1 - 9; og b) indvinding af det heterologe exoprotein.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA200301824 | 2003-12-10 | ||
| PCT/DK2004/000859 WO2005056799A1 (en) | 2003-12-10 | 2004-12-10 | A cell with improved secretion mediated by mrga protein or homologue |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK1694846T3 true DK1694846T3 (da) | 2014-11-17 |
Family
ID=34673548
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK04803012.6T DK1694846T3 (da) | 2003-12-10 | 2004-12-10 | Celle med forbedret sekretion formidlet af MrgA protein eller en homolog |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US8268583B2 (da) |
| EP (1) | EP1694846B1 (da) |
| CN (1) | CN1969045A (da) |
| DK (1) | DK1694846T3 (da) |
| WO (1) | WO2005056799A1 (da) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005123915A1 (en) | 2004-06-21 | 2005-12-29 | Novozymes A/S | Stably maintained multiple copies of at least two orf in the same orientation |
| CN108384770B (zh) * | 2018-03-01 | 2019-11-22 | 江南大学 | 一种降低环糊精对普鲁兰酶抑制作用的方法 |
| CN112662580B (zh) * | 2019-11-27 | 2021-12-24 | 中国农业科学院生物技术研究所 | 一种嗜氧类芽孢杆菌及其应用 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| DK639689D0 (da) | 1989-12-18 | 1989-12-18 | Novo Nordisk As | Indfoering af dna i celler |
| FI91698C (fi) | 1992-07-01 | 1994-07-25 | Nokia Telecommunications Oy | Menetelmä synkronisessa digitaalisessa tietoliikennejärjestelmässä käytettävän signaalin vastaanottamiseksi |
| WO1994019471A1 (en) | 1993-02-26 | 1994-09-01 | The Finnish National Public Health Institute | Method and system for enhanced production of commercially important exoproteins in gram-positive bacteria |
| CA2191718A1 (en) | 1994-06-03 | 1995-12-14 | Randy M. Berka | Phosphonyldipeptides useful in the treatment of cardiovascular diseases |
| AU741600B2 (en) | 1997-06-27 | 2001-12-06 | Plant Genetic Systems N.V. | Improved bacillus thuringiensis toxin |
| CA2296689C (en) | 1997-07-16 | 2014-04-01 | Genencor International, Inc. | Increasing production of proteins in gram-positive microorganisms |
| EP1297170B1 (en) | 2000-06-23 | 2016-03-02 | Novozymes A/S | Method for stable chromosomal multi-copy integration of genes |
-
2004
- 2004-12-10 DK DK04803012.6T patent/DK1694846T3/da active
- 2004-12-10 EP EP04803012.6A patent/EP1694846B1/en not_active Expired - Lifetime
- 2004-12-10 CN CNA2004800415521A patent/CN1969045A/zh active Pending
- 2004-12-10 US US10/582,277 patent/US8268583B2/en not_active Expired - Fee Related
- 2004-12-10 WO PCT/DK2004/000859 patent/WO2005056799A1/en not_active Ceased
-
2012
- 2012-08-10 US US13/571,777 patent/US8535911B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP1694846A1 (en) | 2006-08-30 |
| US20130217097A1 (en) | 2013-08-22 |
| US8268583B2 (en) | 2012-09-18 |
| US20070130630A1 (en) | 2007-06-07 |
| EP1694846B1 (en) | 2014-10-01 |
| WO2005056799A1 (en) | 2005-06-23 |
| CN1969045A (zh) | 2007-05-23 |
| US8535911B2 (en) | 2013-09-17 |
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