DK169975B1 - Rekombinant DNA-molekyle, ekspressionsvektor omfattende samme, værtscelle transformeret med ekspressionsvektoren, fusionsprotein og fremgangsmåde til frembringelse af samme - Google Patents

Rekombinant DNA-molekyle, ekspressionsvektor omfattende samme, værtscelle transformeret med ekspressionsvektoren, fusionsprotein og fremgangsmåde til frembringelse af samme Download PDF

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Publication number: DK169975B1
Authority: DK; Denmark
Prior art keywords: glutathione; fusion protein; enzyme; protein; expression vector
Prior art date: 1987-05-28

Application number

DK038189A

Other languages

Danish (da)

English (en)

Other versions

DK38189D0 (da

DK38189A (da

Inventor

Danold Bruce Smith

Original Assignee

Amrad Corp Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1987-05-28

Filing date

1989-01-27

Publication date

1995-04-18

1989-01-27 Application filed by Amrad Corp Ltd filed Critical Amrad Corp Ltd

1989-01-27 Publication of DK38189D0 publication Critical patent/DK38189D0/da

1989-01-27 Publication of DK38189A publication Critical patent/DK38189A/da

1995-04-18 Application granted granted Critical

1995-04-18 Publication of DK169975B1 publication Critical patent/DK169975B1/da

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210000004899 c-terminal region Anatomy 0.000 description 1
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235000019800 disodium phosphate Nutrition 0.000 description 1
VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 1
239000003623 enhancer Substances 0.000 description 1
238000012869 ethanol precipitation Methods 0.000 description 1
DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
102000013165 exonuclease Human genes 0.000 description 1
210000003714 granulocyte Anatomy 0.000 description 1
230000001900 immune effect Effects 0.000 description 1
230000028993 immune response Effects 0.000 description 1
238000003018 immunoassay Methods 0.000 description 1
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230000002401 inhibitory effect Effects 0.000 description 1
230000000977 initiatory effect Effects 0.000 description 1
238000002955 isolation Methods 0.000 description 1
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108020004707 nucleic acids Proteins 0.000 description 1
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239000010452 phosphate Substances 0.000 description 1
102000020233 phosphotransferase Human genes 0.000 description 1
230000004962 physiological condition Effects 0.000 description 1
108010011110 polyarginine Proteins 0.000 description 1
239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
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230000028327 secretion Effects 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
239000003998 snake venom Substances 0.000 description 1
238000002791 soaking Methods 0.000 description 1
AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
229910000162 sodium phosphate Inorganic materials 0.000 description 1
235000010339 sodium tetraborate Nutrition 0.000 description 1
239000002195 soluble material Substances 0.000 description 1
239000002904 solvent Substances 0.000 description 1
241000894007 species Species 0.000 description 1
239000000021 stimulant Substances 0.000 description 1
239000000126 substance Substances 0.000 description 1
229960002180 tetracycline Drugs 0.000 description 1
229930101283 tetracycline Natural products 0.000 description 1
235000019364 tetracycline Nutrition 0.000 description 1
150000003522 tetracyclines Chemical class 0.000 description 1
HODRFAVLXIFVTR-RKDXNWHRSA-N tevenel Chemical compound NS(=O)(=O)C1=CC=C([C@@H](O)[C@@H](CO)NC(=O)C(Cl)Cl)C=C1 HODRFAVLXIFVTR-RKDXNWHRSA-N 0.000 description 1
229960003766 thrombin (human) Drugs 0.000 description 1
230000001988 toxicity Effects 0.000 description 1
231100000419 toxicity Toxicity 0.000 description 1
230000014621 translational initiation Effects 0.000 description 1
BSVBQGMMJUBVOD-UHFFFAOYSA-N trisodium borate Chemical compound [Na+].[Na+].[Na+].[O-]B([O-])[O-] BSVBQGMMJUBVOD-UHFFFAOYSA-N 0.000 description 1
GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
238000002525 ultrasonication Methods 0.000 description 1
238000002604 ultrasonography Methods 0.000 description 1
238000011144 upstream manufacturing Methods 0.000 description 1
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238000001262 western blot Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
- C12N9/1088—Glutathione transferase (2.5.1.18)
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43536—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from worms
- C07K14/43559—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from worms from trematodes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/23—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a GST-tag
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site

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Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Molecular Biology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biomedical Technology (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Biotechnology (AREA)
Biophysics (AREA)
Microbiology (AREA)
Tropical Medicine & Parasitology (AREA)
Medicinal Chemistry (AREA)
Physics & Mathematics (AREA)
Gastroenterology & Hepatology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Toxicology (AREA)
Plant Pathology (AREA)
Peptides Or Proteins (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Enzymes And Modification Thereof (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

DK038189A 1987-05-28 1989-01-27 Rekombinant DNA-molekyle, ekspressionsvektor omfattende samme, værtscelle transformeret med ekspressionsvektoren, fusionsprotein og fremgangsmåde til frembringelse af samme DK169975B1 (da)

Applications Claiming Priority (6)

Application Number	Priority Date	Filing Date	Title
AUPI219587		1987-05-28
AUPI219587		1987-05-28
AU17932/88A AU607511B2 (en)	1987-05-28	1988-05-27	Novel fusion proteins containing glutathione-s-transferase
PCT/AU1988/000164 WO1988009372A1 (en)	1987-05-28	1988-05-27	Novel fusion proteins containing glutathione-s-transferase
AU8800164		1988-05-27
AU1793288		1988-05-27

Publications (3)

Publication Number	Publication Date
DK38189D0 DK38189D0 (da)	1989-01-27
DK38189A DK38189A (da)	1989-01-27
DK169975B1 true DK169975B1 (da)	1995-04-18

Family

ID=3772204

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DK038189A DK169975B1 (da)	1987-05-28	1989-01-27	Rekombinant DNA-molekyle, ekspressionsvektor omfattende samme, værtscelle transformeret med ekspressionsvektoren, fusionsprotein og fremgangsmåde til frembringelse af samme

Country Status (14)

Country	Link
EP (1)	EP0293249B1 (de)
JP (1)	JPH0681596B2 (de)
AT (1)	ATE79902T1 (de)
AU (1)	AU607511B2 (de)
CA (1)	CA1338903C (de)
DE (1)	DE3873989T2 (de)
DK (1)	DK169975B1 (de)
ES (1)	ES2045115T3 (de)
GR (1)	GR3006173T3 (de)
NO (1)	NO178894C (de)
NZ (1)	NZ224663A (de)
SG (1)	SG25093G (de)
WO (1)	WO1988009372A1 (de)
ZA (1)	ZA883681B (de)

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CA2045663A1 (en) *	1989-01-31	1990-08-01	Sonia Louise Crameri	Vaccine for the preventative treatment of infection of liver fluke in ruminants
USRE37919E1 (en)	1989-05-12	2002-12-03	The General Hospital Corporation	Recombinant DNA method for production of parathyroid hormone
US5171670A (en) *	1989-05-12	1992-12-15	The General Hospital Corporation	Recombinant dna method for production of parathyroid hormone
FR2657883A1 (de) *	1990-02-07	1991-08-09	Transgene Sa
US6077694A (en) *	1990-09-21	2000-06-20	The United States Of America As Represented By The Department Of Health And Human Services	Method for over-expression and rapid purification of biosynthetic proteins
WO1993003157A1 (en) *	1991-07-29	1993-02-18	Dana Farber Cancer Institute	Plasmids for the rapid preparation of modified proteins
US5596079A (en) *	1991-12-16	1997-01-21	Smith; James R.	Mimetics of senescent cell derived inhibitors of DNA synthesis
US6372249B1 (en)	1991-12-16	2002-04-16	Baylor College Of Medicine	Senscent cell-derived inhibitors of DNA synthesis
CA2132321A1 (en) *	1992-03-18	1993-09-30	Donald B. Smith	Tripartite fusion proteins of glutathione s-transferase
GB9210337D0 (en) *	1992-05-14	1992-07-01	Univ Court Of The University O	Feline leukaemia virus vaccine
WO1994017813A1 (en) *	1993-02-08	1994-08-18	Paravax, Inc.	Defective sindbis virus vectors that express toxoplasma gondii p30 antigens
US5532142A (en) *	1993-02-12	1996-07-02	Board Of Regents, The University Of Texas System	Method of isolation and purification of fusion polypeptides
US5480864A (en) *	1993-08-06	1996-01-02	Shiseido Co., Ltd.	Modified maxadilan protein, its preparation and use, and DNA encoding the protein
AU692794B2 (en) *	1993-08-30	1998-06-18	Baylor College Of Medicine	Senescent cell-derived inhibitors of DNA synthesis
US6303369B1 (en) *	1993-09-30	2001-10-16	Carl Spana	Protein expression system
GB9502489D0 (en) *	1995-02-09	1995-03-29	Animal Health Trust	Expression of the non-structural protein NS1 of influenza virus and detection of anti-NS1 antibody in serum
US5800811A (en) *	1995-06-06	1998-09-01	Hall; Frederick L.	Artificial skin prepared from coclagen matrix containing transforming growth factor-β having a collagen binding site
GB9513683D0 (en)	1995-07-05	1995-09-06	Ciba Geigy Ag	Products
SG49993A1 (en)	1995-12-28	1998-06-15	Fujirebio Kk	Fused dna sequence fused protein expressed from said fused dna sequence and method for expressing said fused protein
DE19653498C1 (de) *	1996-12-20	1998-07-23	Wolf M Prof Dr Bertling	Klonierungsvektor-System mit Fusionssequenz
EP1001987B1 (de) *	1997-08-01	2010-12-15	Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.	Zusammensetzung und verfahren zum nachweis von krankheiten in zusammenhang mit der bildung von amyloid-ähnlichen fibrillen oder protein-aggregaten
US6277399B1 (en)	1997-10-31	2001-08-21	New Horizon Diagnostics Corporation	Composition incorporating bacterial phage associated lysing enzymes for treating dermatological infections
US6423299B1 (en)	1997-10-31	2002-07-23	Vincent Fischetti	Composition for treatment of a bacterial infection of an upper respiratory tract
US6406692B1 (en)	1997-10-31	2002-06-18	New Horizons Diagnostics Corp	Composition for treatment of an ocular bacterial infection
US6056954A (en)	1997-10-31	2000-05-02	New Horizons Diagnostics Corp	Use of bacterial phage associated lysing enzymers for the prophylactic and therapeutic treatment of various illnesses
US6432444B1 (en)	1997-10-31	2002-08-13	New Horizons Diagnostics Corp	Use of bacterial phage associated lysing enzymes for treating dermatological infections
US7232576B2 (en)	1997-10-31	2007-06-19	New Horizons Diagnostics Corp	Throat lozenge for the treatment of Streptococcus Group A
US6428784B1 (en)	1997-10-31	2002-08-06	New Horizons Diagnostics Corp	Vaginal suppository for treating group B Streptococcus infection
US6752988B1 (en)	2000-04-28	2004-06-22	New Horizons Diagnostic Corp	Method of treating upper resiratory illnesses
US6077689A (en)	1997-12-24	2000-06-20	Amgen Inc.	Enhanced solubility of recombinant proteins
AU4710899A (en) *	1998-06-24	2000-01-10	Boyce Thompson Institute For Plant Research Inc.	A novel invertebrate intestinal mucin cdna and related products and methods
US6187558B1 (en)	1998-06-24	2001-02-13	Boyce Thompson Institute For Plant Research, Inc.	Invertebrate intestinal mucin cDNA and related products and methods
US7595199B1 (en)	1998-07-31	2009-09-29	Max-Planck-Gesellschaft zur Förderung der Wissenchaften e.V.	Method of detecting amyloid-like fibrils or protein aggregates
GB9821821D0 (en) *	1998-10-07	1998-12-02	Univ Wales	Schistomiasis vaccine
US7063837B2 (en)	1999-09-14	2006-06-20	New Horizons Diagnostics Corp	Syrup composition containing phage associated lytic enzymes
US6632638B1 (en)	2000-11-17	2003-10-14	Amgen, Inc.	Enhanced solubility of recombinant proteins using Uracil DNA glycosylase inhibitor
DK1402015T3 (da)	2001-06-22	2011-12-05	Hoffmann La Roche	Opløseligt kompleks omfattende et retroviralt overfladeglycoprotein og FkpA eller SlyD
US6962982B2 (en)	2001-06-22	2005-11-08	Roche Diagnostics Corporation	Soluble complexes of target proteins and peptidyl prolyl isomerase chaperones and methods of making and using them
US7094757B2 (en)	2001-06-22	2006-08-22	Roche Diagnostics Corporation	Complexes comprising a prion protein and a peptidyl prolyl isomerase chaperone, and method for producing and using them
ES2255347B1 (es) *	2003-05-21	2007-05-16	Universidad De Vigo	Proteina de fusion gst-fucosidasa con actividad enzimatica alfa-l-fucosidasica. proteina recombinante alfa-l-fucosidasa humana.
MY160430A (en) *	2008-12-12	2017-03-15	Univ Sains Malaysia	Recombinant antigen for detection of toxocariasis
GB0823550D0 (en)	2008-12-24	2009-01-28	Kinasource Ltd	Peptide tag and uses thereof

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AU3036684A (en) *	1983-07-07	1985-01-10	Genex Corp.	Production of bovine calf chymosin
MA20977A1 (fr) *	1986-05-19	1987-12-31	Ciba Geigy Ag	Plantes tolerant les herbicides contenant le gene de gluthathione S-Transferase
AU613583B2 (en) *	1986-08-22	1991-08-08	Transgene S.A.	Proteins and the method for preparing them, DNA sequences, antibodies and their application, poxviruses, transformed or infected cells, pharmaceutical compositions which are useful in the prevention of schistosomiasis and application by way of an agent possessing glutathione s-transferase activity
AU603145B2 (en) *	1986-10-14	1990-11-08	Shionogi & Co., Ltd.	Aminoglycoside phosphotransferase-proteinfusions

1988
- 1988-05-17 NZ NZ224663A patent/NZ224663A/en unknown
- 1988-05-24 ZA ZA883681A patent/ZA883681B/xx unknown
- 1988-05-25 CA CA000567639A patent/CA1338903C/en not_active Expired - Lifetime
- 1988-05-27 AT AT88304868T patent/ATE79902T1/de not_active IP Right Cessation
- 1988-05-27 WO PCT/AU1988/000164 patent/WO1988009372A1/en not_active Ceased
- 1988-05-27 ES ES88304868T patent/ES2045115T3/es not_active Expired - Lifetime
- 1988-05-27 AU AU17932/88A patent/AU607511B2/en not_active Expired
- 1988-05-27 JP JP63504420A patent/JPH0681596B2/ja not_active Expired - Lifetime
- 1988-05-27 DE DE8888304868T patent/DE3873989T2/de not_active Expired - Lifetime
- 1988-05-27 EP EP88304868A patent/EP0293249B1/de not_active Expired - Lifetime
1989
- 1989-01-26 NO NO890325A patent/NO178894C/no not_active IP Right Cessation
- 1989-01-27 DK DK038189A patent/DK169975B1/da not_active IP Right Cessation
1992
- 1992-11-05 GR GR920402494T patent/GR3006173T3/el unknown
1993
- 1993-03-06 SG SG250/93A patent/SG25093G/en unknown

Also Published As

Publication number	Publication date
AU1793288A (en)	1988-12-21
DK38189D0 (da)	1989-01-27
JPH0681596B2 (ja)	1994-10-19
SG25093G (en)	1993-06-11
ZA883681B (en)	1989-07-26
NO178894C (no)	1996-06-26
EP0293249A1 (de)	1988-11-30
NO890325L (no)	1989-03-06
NO890325D0 (no)	1989-01-26
EP0293249B1 (de)	1992-08-26
CA1338903C (en)	1997-02-11
WO1988009372A1 (en)	1988-12-01
ES2045115T3 (es)	1994-01-16
NO178894B (no)	1996-03-18
AU607511B2 (en)	1991-03-07
DE3873989D1 (de)	1992-10-01
JPH01503441A (ja)	1989-11-22
NZ224663A (en)	1990-11-27
DE3873989T2 (de)	1993-02-04
GR3006173T3 (de)	1993-06-21
ATE79902T1 (de)	1992-09-15
DK38189A (da)	1989-01-27

Legal Events

Date	Code	Title	Description
1996-12-16	AHB	Application shelved due to non-payment
2008-06-16	PUP	Patent expired

Publication	Publication Date	Title
DK169975B1 (da)	1995-04-18	Rekombinant DNA-molekyle, ekspressionsvektor omfattende samme, værtscelle transformeret med ekspressionsvektoren, fusionsprotein og fremgangsmåde til frembringelse af samme
US5654176A (en)	1997-08-05	Fusion proteins containing glutathione-s-transferase
Smith et al.	1988	Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase
EP0722461B1 (de)	2004-10-20	Expression eines fusionspolypeptides, das ohne leadersequenz aus dem cytoplasma transportiert wird
Kaster et al.	1983	Analysis of a bacterial hygromycin B resistance gene by transcriptional and translational fusions and by DNA sequencing
JP3250968B2 (ja)	2002-01-28	オリゴペプチド反復単位を有する大ポリペプチド
US5641650A (en)	1997-06-24	Expression of heterologous polypeptides in halobacteria
Baty et al.	1987	Extracellular release of colicin A is non‐specific.
IE57069B1 (en)	1992-04-22	Dna sequences,recombinant dna molecules and processes for producing human fibroblast interferon
JPS62500700A (ja)	1987-03-26	ストレプトアビジン様ポリペプチドの製造
Davies et al.	1993	Recombinant baculovirus vectors expressing glutathione–S–transferase fusion proteins
WO1984003519A1 (en)	1984-09-13	Modified signal peptides
CN104583408B (zh)	2017-09-22	用于降低免疫测定的干扰且稳定免疫测定的分子伴侣‑分子伴侣融合多肽
US4659669A (en)	1987-04-21	Microbial expression of human influenza hemagglutinin proteins
EP0181634A2 (de)	1986-05-21	Synthetisches Gen für menschliches Lysozym
JPS63267278A (ja)	1988-11-04	インタ−フエロン結合体を暗号化する塩基配列
Elleman et al.	1986	Nucleotide sequence of the gene encoding the two-subunit pilin of Bacteroides nodosus 265
Luojing et al.	1991	Adsorption to starch of a β-galactosidase fusion protein containing the starch-binding region of Aspergillus glucoamylase
US5763284A (en)	1998-06-09	Methods for peptide synthesis and purification
EP0321940B1 (de)	1994-02-16	Methode zur Herstellung von natürlichen, menschlichen Wachstumshormon in reiner Form
EP1042467B1 (de)	2008-08-06	Nicht identische gene und ihre anwendung in verbesserten molekularen adjuvanten
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