DK181720B1 - Antiparasiticid formulering af bacillus thuringiensis-sporer, anvendelse af formuleringen til et veterinært medikament og et akvakulturelt parasiticidt kit - Google Patents

Antiparasiticid formulering af bacillus thuringiensis-sporer, anvendelse af formuleringen til et veterinært medikament og et akvakulturelt parasiticidt kit Download PDF

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DK181720B1
DK181720B1 DKPA202270092A DKPA202270092A DK181720B1 DK 181720 B1 DK181720 B1 DK 181720B1 DK PA202270092 A DKPA202270092 A DK PA202270092A DK PA202270092 A DKPA202270092 A DK PA202270092A DK 181720 B1 DK181720 B1 DK 181720B1
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Vargas Deborah
María Sandino Ana
Rioseco Claudia
Arenillas Esteban
Fuentes Yazmín
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Consorcio Tecnologico De Sanidad Acuicola S A
Univ Santiago Chile
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A01N63/23—B. thuringiensis
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P15/00—Biocides for specific purposes not provided for in groups A01P1/00 - A01P13/00

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
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  • Agronomy & Crop Science (AREA)
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  • Dentistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Claims (3)

DK 181720 B1 52 PATENTKRAV
1. Akvakulturel parasiticid formulering mod havlus der tilhører Caligus-slæg- terne, hvilken formulering omfatter: a) sporer af en Bacillus thuringiensis (Bt) subsp. Kurstaki stamme ABTS- 351; og b) det rekombinante Cry1Ab (SEQ ID NO:2) protein.
2. Akvakulturel parasiticid formulering ifølge krav 1, hvilken formulering omfat- ter 150 mg/ml af det rekombinante protein Cry 1Ab.
3. Akvakulturel parasiticid formulering ifølge et hvilket som helst af kravene 1 eller 2, hvor sporerne der er indeholdt i formuleringen, er 1x10% to 1x10'° CFU/mL.
4. Akvakulturel parasiticid formulering ifølge krav 3, hvor sporerne der er inde- holdt i formuleringen, er 1x108 CFU/mL.
5. Veterinær akvakulturel parasiticid sammensætning, hvilken sammensæt- ning omfatter: - formuleringen ifølge et hvilket som helst af kravene 1 til 4; og - en veterinær acceptabel bærer.
6. Veterinær akvakulturel parasiticid sammensætning ifølge krav 5, hvilken sammensætning er i en form, der er valgt fra gruppen, som består af: nedsænk- ningsbad, injicerbar formulering, foderformel.
7. Anvendelse af formuleringen ifølge et hvilket som helst af kravene 1 til 4 til fremstilling af et veterinærlægemiddel, der er egnet til behandling af fisk, som er inficeret med en havluseparasit, hvilken havlus er en Caligus rogercresseyi-art .
8. Anvendelse af sammensætningen ifølge et hvilket som helst af kravene 5 eller 6 til fremstilling af et veterinærlægemiddel, der er egnet til behandling af fisk, som er inficeret med en havluseparasit, hvilken havlus er en Caligus rogercres- seyl-art .
DK 181720 B1 53
9. Akvakulturelt parasiticidt kit, hvilket kit omfatter en eller flere beholdere, der indeholder den akvakulturelle parasiticide formulering, som omfatter sporer af Bacillus thuringiensis subsp. Kurstaki stamme ABTS-351; og det Cry1Ab-rekom- binante protein, ifølge et hvilket som helst af kravene 1 til 4, hvilket kit yderligere omfatter en brugsanvisning.
DK 181720 B1 SEQUENCE LISTING <110> Universidad de Santiago de Chile ICTIO Biotechnologies S.A. <120> Antiparasitic formulation of spores and/or proteins of Bacillus thuringiensis for the treatment of parasites of the Caligidae family in fish <160> 8 <170> PatentIn version 3.5 <210> 1 <211> 3540 <212> DNA <213> Bacillus thuringiensis <400> 1 catatggata acaatccgaa catcaatgaa tgcattcctt ataattgttt aagtaaccct 60 gaagtagaag tattaggtgg agaaagaata gaaactggtt acaccccaat cgatatttcc 120 ttgtcgctaa cgcaatttct tttgagtgaa tttgttcccg gtgctggatt tgtgttagga 180 ctagttgata taatatgggg aatttttggt ccctctcaat gggacgcatt tcttgtacaa 240 attgaacagt taattaacca aagaatagaa gaattcgcta ggaaccaagc catttctaga 300 ttagaaggac taagcaatct ttatcaaatt tacgcagaat cttttagaga gtgggaagca 360 gatcctacta atccagcatt aagagaagag atgcgtattc aattcaatga catgaacagt 420 gcccttacaa ccgctattcc tctttttgca gttcaaaatt atcaagttcc tcttttatca 480 gtatatgttc aagctgcaaa tttacattta tcagttttga gagatgtttc agtgtttgga 540 caaaggtggg gatttgatgc cgcgactatc aatagtcgtt ataatgattt aactaggctt 600 attggcaact atacagatta tgctgtacgc tggtacaata cgggattaga gcgtgtatgg 660 ggaccggatt ctagagattg gataagatat aatcaattta gaagagaatt aacactaact 720 gtattagata tcgtttctct atttccgaac tatgatagta gaacgtatcc aattcgaaca 780 gtttcccaat taacaagaga aatttataca aacccagtat tagaaaattt tgatggtagt 840 tttcgaggct cggctcaggg catagaagga agtattagga gtccacattt gatggatata 900 cttagcagta taaccatcta tacggatgct catagaggag aatattattg gtcagggcat 960 caaataatgg cttctcctgt agggttttcg gggccagaat tcacttttcc gctatatgga 1020 actatgggaa atgcagctcc acaacaacgt attgttgctc aactaggtca gggcgtgtat 1080 agaacattat cgtccacttt atatagaaga ccttttaata tagggataaa taatcaacaa 1140 ctatctgttc ttgacgggac agaatttgct tatgggacct cctcaaattt gccatccgct 1200 gtatacagaa aaagcggaac ggtagattcg ctggatgaaa taccaccaca gaataacaac 1260 gtgccaccta ggcaaggatt tagtcatcga ttaagccatg tttcaatgtt tcgttcaggc 1320 tttagtaata gtagtgtaag tataataaga gctcctatgt tctcttggat acatcgtagt 1380 gctgaattta ataatataat tccttcatca caaattacac aaataccttt aacaaaatct 1440 actaatcttg gctctggaac ttctgtcgtt aaaggaccag gatttacagg aggagatatt 1500 cttcgaagaa cttcacctgg ccagatttca accttaagag taaatattac tgcaccatta 1560 tcacaaagat atcgggtaag aattcgctac gcttctacta caaatttaca attccataca 1620 tcaattgacg gaagacctat taatcagggg aatttttcag caactatgag tagtgggagt 1680 aatttacagt ccggaagctt taggactgta ggttttacta ctccgtttaa cttttcaaat 1740 ggatcaagtg tatttacgtt aagtgctcat gtcttcaatt caggcaatga agtttatata 1860 gatcgaattg aatttgttcc ggcagaagta acctttgagg cagaatatga tttagaaaga 1860 gcacaaaagg cggtgaatga gctgtttact tcttccaatc aaatcgggtt aaaaacagat 1920 gtgacggatt atcatattga tcaagtatcc aatttagttg agtgtttatc agatgaattt 1980 tgtctggatg aaaaacaaga agtgtccgag aaagtcaaac atgcgaagcg acttagtgat 2040 gagcggaatt tacttcaaga tccaaacttc agagggatca atagacaact agaccgtggc 2100 tggagaggaa gtacggatat taccatccaa ggaggcgatg acgtattcaa agagaattac 2160 gttacactac caggtacctt tgatgagtgc tatccaacgt atttatatca aaaaatagat 2220 gagtcgaaat taaaagccta tacccgttat caattaagag ggtatatcga ggatagtcaa 2280 gacttagaaa tctatttaat tcgctacaat gcaaaacatg aaacagtaaa tgtgccaggt 2340 acgggttcct tatggccget ttcagcccaa agtccaatcg gaaagtgtgg agagccgaat 2400 cgatgcgcgc cacaccttga atggaatcct gacttagatt gttcgtgtag ggatggagaa 2460 aagtgtgccc atcattcgca tcatttctcc ttagacattg atgttggatg tacagactta 2520 aatgaggacc taggtgtatg ggtgatcttt aagattaaga cgcaagatgg gcacgcaaga 2580 ctagggaatc tagagtttct cgaagagaaa ccattagtag gagaagcgct agctcgtgtg 2640 aaaagagcgg agaaaaaatg gagagacaaa cgcgaaaaat tggaatggga aacaaatatc 2700 gtttataaag aggcaaaaga atctgtagat gctttatttg taaactctca atatgatcaa 2760 ttacaagcgg atacgaatat tgccatgatt catgcggcag ataaacgtgt tcatagcatt 2820 cgagaagctt atctgcctga gctgtctgtg attccgggtg tcaatgcggc tatttttgaa 2880 gaattagaag ggtgtatttt cactgcattc tccctatatg atgcgagaaa tgtcattaaa 2940 aatggtgatt ttaataatgg cttatcctgc tggaacgtga aagggcatgt agatgtagaa 3000 gaacaaaaca accaccgttc ggtccttgtt gttccggaat gggaagcaga agtgtcacaa 3060
DK 181720 B1 gaagttcgtg tctgtccggg tcgtggctat atccttcgtg tcacagcgta caaggaggga 3120 tatggagaag gttgcgtaac cattcatgag atcgagaaca atacagacga actgaagttt 3180 agcaactgcg tagaagagga aatctatcca aacaacacgg taacgtgtaa tgattatact 3240 gtaaatcaag aagaatacga aggtgcgtac acttctcgta atcgaggata taacgaagct 3300 ccttccgtac cagctgatta tgcatcagtc tatgaagaaa aatcgtatac agatggacga 3360 agagagaatc cttgtgaatt taacagaggg tatagggatt acacgctact accagttggt 3420 tatgtgacaa aagaattaga atacttccca gaaaccgata aggtatggat tgagattgga 3480 gaaacggaag gaacatttat cgtggacagc gtggaattac tcctaatgga ggaactcgag 3540 <210> 2 <211> 1179 <212> PRT <213> Bacillus thuringiensis <400> 2 Met Asp Asn Asn Pro Asn Ile Asn Glu Cys Ile Pro Tyr Asn Cys Leu 1510 15 Ser Asn Pro Glu Val Glu Val Leu Gly Gly Glu Arg Ile Glu Thr Gly
Tyr Thr Pro Ile Asp Ile Ser Leu Ser Leu Thr Gln Phe Leu Leu Ser
Glu Phe Val Pro Gly Ala Gly Phe Val Leu Gly Leu Val Asp Ile Ile 60 Trp Gly Ile Phe Gly Pro Ser Gln Trp Asp Ala Phe Leu Val Gln Ile 65 70 75 80 Glu Gln Leu Ile Asn Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala 85 90 95 Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Gln Ile Tyr Ala Glu 100 105 110 Ser Phe Arg Glu Trp Glu Ala Asp Pro Thr Asn Pro Ala Leu Arg Glu 115 120 125 Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Thr Thr Ala 130 135 140 Ile Pro Leu Phe Ala Val Gln Asn Tyr Gln Val Pro Leu Leu Ser Val 145 150 155 160 Tyr Val Gln Ala Ala Asn Leu His Leu Ser Val Leu Arg Asp Val Ser 165 170 175 Val Phe Gly Gln Arg Trp Gly Phe Asp Ala Ala Thr Ile Asn Ser Arg 180 185 190 Tyr Asn Asp Leu Thr Arg Leu Ile Gly Asn Tyr Thr Asp Tyr Ala Val 195 200 205 Arg Trp Tyr Asn Thr Gly Leu Glu Arg Val Trp Gly Pro Asp Ser Arg 210 215 220 Asp Trp Ile Arg Tyr Asn Gln Phe Arg Arg Glu Leu Thr Leu Thr Val 225 230 235 240 Leu Asp Ile Val Ser Leu Phe Pro Asn Tyr Asp Ser Arg Thr Tyr Pro 245 250 255 Ile Arg Thr Val Ser Gln Leu Thr Arg Glu Ile Tyr Thr Asn Pro Val 260 265 270 Leu Glu Asn Phe Asp Gly Ser Phe Arg Gly Ser Ala Gln Gly Ile Glu 275 280 285 Gly Ser Ile Arg Ser Pro His Leu Met Asp Ile Leu Ser Ser Ile Thr 290 295 300 Ile Tyr Thr Asp Ala His Arg Gly Glu Tyr Tyr Trp Ser Gly His Gln 305 310 315 320 Ile Met Ala Ser Pro Val Gly Phe Ser Gly Pro Glu Phe Thr Phe Pro 325 330 335 Leu Tyr Gly Thr Met Gly Asn Ala Ala Pro Gin Gin Arg Ile Val Ala 340 345 350 Gln Leu Gly Gln Gly Val Tyr Arg Thr Leu Ser Ser Thr Leu Tyr Arg 355 360 365 Arg Pro Phe Asn Ile Gly Ile Asn Asn Gln Gln Leu Ser Val Leu Asp 370 375 380 Gly Thr Glu Phe Ala Tyr Gly Thr Ser Ser Asn Leu Pro Ser Ala Val 385 390 395 400 Tyr Arg Lys Ser Gly Thr Val Asp Ser Leu Asp Glu Ile Pro Pro Gin
DK 181720 B1
405 410 415
Asn Asn Asn Val Pro Pro Arg Gln Gly Phe Ser His Arg Leu Ser His 420 425 430
Val Ser Met Phe Arg Ser Gly Phe Ser Asn Ser Ser Val Ser Ile Ile 435 440 445
Arg Ala Pro Met Phe Ser Trp Ile His Arg Ser Ala Glu Phe Asn Asn 450 455 460
Ile Ile Pro Ser Ser Gln Ile Thr Gln Ile Pro Leu Thr Lys Ser Thr 465 470 475 480
Asn Leu Gly Ser Gly Thr Ser Val Val Lys Gly Pro Gly Phe Thr Gly 485 490 495
Gly Asp Ile Leu Arg Arg Thr Ser Pro Gly Gln Ile Ser Thr Leu Arg 500 505 510
Val Asn Ile Thr Ala Pro Leu Ser Gln Arg Tyr Arg Val Arg Ile Arg 515 520 525
Tyr Ala Ser Thr Thr Asn Leu Gln Phe His Thr Ser Ile Asp Gly Arg 530 535 540
Pro Ile Asn Gln Gly Asn Phe Ser Ala Thr Met Ser Ser Gly Ser Asn 545 550 555 560
Leu Gln Ser Gly Ser Phe Arg Thr Val Gly Phe Thr Thr Pro Phe Asn 565 570 575
Phe Ser Asn Gly Ser Ser Val Phe Thr Leu Ser Ala His Val Phe Asn 580 585 590
Ser Gly Asn Glu Val Tyr Ile Asp Arg Ile Glu Phe Val Pro Ala Glu 595 600 605
Val Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Ala Val 610 615 620
Asn Glu Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val 625 630 635 640
Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu Ser 645 650 655
Asp Glu Phe Cys Leu Asp Glu Lys Gln Glu Val Ser Glu Lys Val Lys 660 665 670
His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn 675 680 685
Phe Arg Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp Arg Gly Ser Thr 690 695 700
Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 705 710 715 720
Thr Leu Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln 725 730 735
Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg 740 745 750
Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr 755 760 765
Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp 770 775 780
Pro Leu Ser Ala Gln Ser Pro Ile Gly Lys Cys Gly Glu Pro Asn Arg 785 790 795 800
Cys Ala Pro His Leu Glu Trp Asn Pro Asp Leu Asp Cys Ser Cys Arg 805 810 815
Asp Gly Glu Lys Cys Ala His His Ser His His Phe Ser Leu Asp Ile 820 825 830
Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val Ile 835 840 845
Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu Glu 850 855 860
Phe Leu Glu Glu Lys Pro Leu Val Gly Glu Ala Leu Ala Arg Val Lys 865 870 875 880
Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Lys Leu Glu Trp Glu 885 890 895
Thr Asn Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu Phe 900 905 910
Val Asn Ser Gln Tyr Asp Gln Leu Gln Ala Asp Thr Asn Ile Ala Met
DK 181720 B1 915 920 925 Ile His Ala Ala Asp Lys Arg Val His Ser Ile Arg Glu Ala Tyr Leu 930 935 940 Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu Glu 945 950 955 960 Leu Glu Gly Cys Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg Asn 965 970 975 Val Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Ser Cys Trp Asn Val 980 985 990 Lys Gly His Val Asp Val Glu Glu Gln Asn Asn His Arg Ser Val Leu 995 1000 1005 Val Val Pro Glu Trp Glu Ala Glu Val Ser Gln Glu Val Arg Val 1010 1015 1020 Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr Ala Tyr Lys Glu 1025 1030 1035 Gly Tyr Gly Glu Gly Cys Val Thr Ile His Glu Ile Glu Asn Asn 1040 1045 1050 Thr Asp Glu Leu Lys Phe Ser Asn Cys Val Glu Glu Glu Ile Tyr 1055 1060 1065 Pro Asn Asn Thr Val Thr Cys Asn Asp Tyr Thr Val Asn Gln Glu 1070 1075 1080 Glu Tyr Glu Gly Ala Tyr Thr Ser Arg Asn Arg Gly Tyr Asn Glu 1085 1090 1095 Ala Pro Ser Val Pro Ala Asp Tyr Ala Ser Val Tyr Glu Glu Lys 1100 1105 1110 Ser Tyr Thr Asp Gly Arg Arg Glu Asn Pro Cys Glu Phe Asn Arg 1115 1120 1125 Gly Tyr Arg Asp Tyr Thr Leu Leu Pro Val Gly Tyr Val Thr Lys 1130 1135 1140 Glu Leu Glu Tyr Phe Pro Glu Thr Asp Lys Val Trp Ile Glu Ile 1145 1150 1155 Gly Glu Thr Glu Gly Thr Phe Ile Val Asp Ser Val Glu Leu Leu 1160 1165 1170 Leu Met Glu Glu Leu Glu 1175 <210> 3 <211> 1956 <212> DNA <213> Bacillus thuringiensis <400> 3 catatggata ataatcccaa cataaacgaa tgtataccat acaactgtct aagcaacccg 60 gaagtggaag tgctgggcgg cgaacgcatc gaaaccggtt acaccccgat cgatattagc 120 ctgagcctga cccagttcct gctgagcgaa tttgttccgg gtgegggttt tgtgetggge 180 ctggttgata tcatttgggg tatcttcggc ccgagccaat gggacgcgtt tctggttcag 240 atcgagcaac tgattaacca gcgtatcgag gaattcgcgc gtaaccaagc gattagccgt 300 ctggagggtc tgagcaacct gtaccagatc tatgcggaga gcttccgtga gtgggaagcg 360 gacccgacca acccggeget gcgtgaggaa atgcgtattc aattcaacga catgaacagc 420 gcgctgacca ccgcgatccc gctgtttgcg gtgcagaact accaagttcc gctgctgagc 480 gtgtatgttc aagcggcgaa cctgcacctg agcgtgctgc gtgatgtgag cgttttcggt 540 caacgttggg gctttgacgc ggcgaccatt aacagccgtt acaacgatct gacccgtctg 600 atcggtaact acaccgacca cgcggtgcgt tggtataaca ccggtctgga gegtgtttgg 660 ggcccggaca gccgtgattg gattcgttat aaccagttcc gtcgtgaact gaccctgacc 720 gtgctggaca tcgttagcct gtttccgaac tacgatagcc gtacctatcc gattcgtacc 780 gtgagccagc tgacccgtga gatctacacc aacccggttc tggaaaactt cgacggtagc 840 tttcgtggca gcgcgcaagg tattgaaggc agcatccgta gcccgcacct gatggatatt 900 ctgaacagca tcaccattta caccgacgcg caccgtggtg agtactattg gagcggtcac 960 cagatcatgg cgagcccggt tggtttcagc ggtccggaat tcacctttcc gctgtatggt 1020 accatgggca acgcggcgec gcagcaacgt attgtggege agctgggtca aggcgtttac 1080 cgtaccctga gcagcaccct gtatcgtcgt ccgttcaaca tcggtattaa caaccagcaa 1140 ctgagcgtgc tggatggtac cgagtttgcg tacggcacca gcagcaacct gccgagcgcg 1200 gtgtatcgta agagcggtac cgttgacagc ctggatgaaa tcccgccgca gaacaacaac 1260 gttccgecge gtcaaggttt tagccaccgt ctgagccacg tgagcatgtt ccgtagcggc 1320 tttagcaaca gcagcgttag catcattcgt gcgccgatgt tcagctggat tcaccgtagc 1380 gcggagttta acaacatcat tgcgagcgat agcatcaccc agattccggc ggtgaagggt 1440
DK 181720 B1 aacttcctgt ttaacggcag cgttattagc ggtccgggtt tcaccggtgg cgacctggtg 1500 cgtctgaaca gcagcggtaa caacatccaa aaccgtggct acattgaagt tccgatccac 1560 tttccgagca ccagcacccg ttaccgtgtg cgtgttcgtt atgcgagcgt gaccccgatt 1620 cacctgaacg ttaactgggg taacagcagc atcttcagca acaccgtgcc ggcgaccgcg 1680 accagcctgg acaacctgca gagcagcgat ttcggctact ttgagagcgc gaacgcgttc 1740 accagcagcc tgggtaacat tgtgggcgtt cgtaacttta gcggtaccgc gggcgtgatc 1800 attgaccgtt tcgagtttat cccggttacc gcgaccctgg aggcggaata taacctggaa 1860 cgtgcgcaaa aggcggttat gccgagcagc ccgctgcaga ccagcctgga tagccgtcaa 1920 atgggcggta gccaccacca tcatcatcat caccac 1956 <210> 4 <211> 651 <212> PRT <213> Bacillus thuringiensis <400> 4 Met Asp Asn Asn Pro Asn Ile Asn Glu Cys Ile Pro Tyr Asn Cys Leu 1510 15 Ser Asn Pro Glu Val Glu Val Leu Gly Gly Glu Arg Ile Glu Thr Gly
Tyr Thr Pro Ile Asp Ile Ser Leu Ser Leu Thr Gln Phe Leu Leu Ser
Glu Phe Val Pro Gly Ala Gly Phe Val Leu Gly Leu Val Asp Ile Ile 60 Trp Gly Ile Phe Gly Pro Ser Gln Trp Asp Ala Phe Leu Val Gln Ile 65 70 75 80 Glu Gln Leu Ile Asn Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala 85 90 95 Ile Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Gln Ile Tyr Ala Glu 100 105 110 Ser Phe Arg Glu Trp Glu Ala Asp Pro Thr Asn Pro Ala Leu Arg Glu 115 120 125 Glu Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Thr Thr Ala 130 135 140 Ile Pro Leu Phe Ala Val Gln Asn Tyr Gln Val Pro Leu Leu Ser Val 145 150 155 160 Tyr Val Gln Ala Ala Asn Leu His Leu Ser Val Leu Arg Asp Val Ser 165 170 175 Val Phe Gly Gln Arg Trp Gly Phe Asp Ala Ala Thr Ile Asn Ser Arg 180 185 190 Tyr Asn Asp Leu Thr Arg Leu Ile Gly Asn Tyr Thr Asp His Ala Val 195 200 205 Arg Trp Tyr Asn Thr Gly Leu Glu Arg Val Trp Gly Pro Asp Ser Arg 210 215 220 Asp Trp Ile Arg Tyr Asn Gln Phe Arg Arg Glu Leu Thr Leu Thr Val 225 230 235 240 Leu Asp Ile Val Ser Leu Phe Pro Asn Tyr Asp Ser Arg Thr Tyr Pro 245 250 255 Ile Arg Thr Val Ser Gln Leu Thr Arg Glu Ile Tyr Thr Asn Pro Val 260 265 270 Leu Glu Asn Phe Asp Gly Ser Phe Arg Gly Ser Ala Gln Gly Ile Glu 275 280 285 Gly Ser Ile Arg Ser Pro His Leu Met Asp Ile Leu Asn Ser Ile Thr 290 295 300 Ile Tyr Thr Asp Ala His Arg Gly Glu Tyr Tyr Trp Ser Gly His Gln 305 310 315 320 Ile Met Ala Ser Pro Val Gly Phe Ser Gly Pro Glu Phe Thr Phe Pro 325 330 335 Leu Tyr Gly Thr Met Gly Asn Ala Ala Pro Gin Gin Arg Ile Val Ala 340 345 350 Gln Leu Gly Gln Gly Val Tyr Arg Thr Leu Ser Ser Thr Leu Tyr Arg 355 360 365 Arg Pro Phe Asn Ile Gly Ile Asn Asn Gln Gln Leu Ser Val Leu Asp 370 375 380 Gly Thr Glu Phe Ala Tyr Gly Thr Ser Ser Asn Leu Pro Ser Ala Val 385 390 395 400
DK 181720 B1 Tyr Arg Lys Ser Gly Thr Val Asp Ser Leu Asp Glu Ile Pro Pro Gln 405 410 415 Asn Asn Asn Val Pro Pro Arg Gln Gly Phe Ser His Arg Leu Ser His 420 425 430 Val Ser Met Phe Arg Ser Gly Phe Ser Asn Ser Ser Val Ser Ile Ile 435 440 445 Arg Ala Pro Met Phe Ser Trp Ile His Arg Ser Ala Glu Phe Asn Asn 450 455 460 Ile Ile Ala Ser Asp Ser Ile Thr Gln Ile Pro Ala Val Lys Gly Asn 465 470 475 480 Phe Leu Phe Asn Gly Ser Val Ile Ser Gly Pro Gly Phe Thr Gly Gly 485 490 495 Asp Leu Val Arg Leu Asn Ser Ser Gly Asn Asn Ile Gln Asn Arg Gly 500 505 510 Tyr Ile Glu Val Pro Ile His Phe Pro Ser Thr Ser Thr Arg Tyr Arg 515 520 525 Val Arg Val Arg Tyr Ala Ser Val Thr Pro Ile His Leu Asn Val Asn 530 535 540 Trp Gly Asn Ser Ser Ile Phe Ser Asn Thr Val Pro Ala Thr Ala Thr 545 550 555 560 Ser Leu Asp Asn Leu Gln Ser Ser Asp Phe Gly Tyr Phe Glu Ser Ala 565 570 575 Asn Ala Phe Thr Ser Ser Leu Gly Asn Ile Val Gly Val Arg Asn Phe 580 585 590 Ser Gly Thr Ala Gly Val Ile Ile Asp Arg Phe Glu Phe Ile Pro Val 595 600 605 Thr Ala Thr Leu Glu Ala Glu Tyr Asn Leu Glu Arg Ala Gln Lys Ala 610 615 620 Val Met Pro Ser Ser Pro Leu Gln Thr Ser Leu Asp Ser Arg Gin Met 625 630 635 640 Gly Gly Ser His His His His His His His His 645 650 <210> 5 <211> 1908 <212> DNA <213> Bacillus thuringiensis <400> 5 catatgaata gtgtattgaa tagcggaaga aatactattt gtgatgcgta taatgtagtg 60 gttcatgatc catttagttt tcaacataaa tcattagata ccatacaaaa agaatggatg 120 gagtggaaaa aagataatca tagtttatat gtagatccta ttgttggaac tgtggctagt 180 tttctgttaa agaaattggg gagccttatt ggaaaacgga tactgagtga attacggaat 240 ttaatatttc ctagtggcag tacaaatcta atggaagata ttttaagaga gacagaaaaa 300 ttcctaaatc aaaaacttaa tacagacact ctttcccgtg taaatgcgga attgacaggg 360 ctgcaagcaa atgtagaaga gtttaatcga caagtagata attttttgaa ccctaaccga 420 aacgctgttc ctttatcaat aacttcttca gttaatacaa tgcagcaatt atttctaaat 480 agattatccc agttccagat gcaaggatac caactgttat tattaccttt atttgcacag 540 gcagccaatt tacatctttc ttttattaga gatgttattc ttaatgcaga agaatggggc 600 atttcagcag caacattacg tacgtatcaa aatcacctga gaaattatac aagagattac 660 tctaattatt gtatagatac gtatcaaact gcgtttagag gtttaaacac ccgtttacac 720 gatatgttag aatttagaac atatatgttt ttaaatgtat ttgaatatgt atctatctgg 780 tcgttgttta aatatcaaag tcttctagta tcttctggcg ctaatttata tgcaagtggt 840 agtggaccac agcagaccca attatttact tcacaagact ggccattttt atattctctt Seo ttccaagtta attcgaatta tgtattatcc ggctttagtg gggctagtct ttttactacc 960 tttcctaata ttggtggctt acctggttct actacaactc aagcattact tgctgcaagg 1020 gttaattata gtggaggaat tacatctggt agtatagggg gttctaattt taatcaaaat 1080 tttaattgca acacgatatc gccacctttg tcaacgtcat ttgttagaag ttggctagat 1140 tcgggttcag atcgacaggg cgttaatacc gttacaaatt ggcaaacaga gtcctttgag 1260 acaacttcag gtttaaggtg tggtgctttt acacctcgtg gtaattcgaa ctattaccct 1260 ggttatttta tccgtaatat ttctggtgtt tctttagttc ttagaaatga agacttaaaa 1320 agaccgttat actataacga aaaaaggaat atagaaagcc cttcaggaac acctggtgga 1380 gcaagagctt atatggtatc tgtgcataac aaaaaaaata acatttatgc agttcatgaa 1440 aatggtacta tgattcattt agcgccggaa gataatacag gatttactat atcaccgata 1500 catgccactc aagtgaataa tcaaacgcga acatttattt ccgaaaaatt tggaaatcaa 1560 ggtgattcct taagatttga acaaagcaac acgacagctc gttataccct tagagggaat 1620
DK 181720 B1 ggaaatagtt acaatcttta tttaagagta tcttcaatag gaaattccac tattcgagtt 1680 actataaacg gtagagttta tactgcttca aatgttaata ctactacaaa taacgatgga 1740 gttaatgata acggagctcg tttttcagat attaatatcg gtaatgtagt agcaagtagt 1860 aattctgatg taccattaga tataaatgta acattaaact ccggtactca atttgatctt 1860 atgaatatta tgcttgtacc aactaatatt tcaccacttt atctcgag 1908 <210> 6 <211> 635 <212> PRT <213> Bacillus thuringiensis <400> 6 Met Asn Ser Val Leu Asn Ser Gly Arg Asn Thr Ile Cys Asp Ala Tyr 1510 15 Asn Val Val Val His Asp Pro Phe Ser Phe Gln His Lys Ser Leu Asp
Thr Ile Gln Lys Glu Trp Met Glu Trp Lys Lys Asp Asn His Ser Leu
Tyr Val Asp Pro Ile Val Gly Thr Val Ala Ser Phe Leu Leu Lys Lys 60 Leu Gly Ser Leu Ile Gly Lys Arg Ile Leu Ser Glu Leu Arg Asn Leu 65 70 75 80 Ile Phe Pro Ser Gly Ser Thr Asn Leu Met Glu Asp Ile Leu Arg Glu 85 90 95 Thr Glu Lys Phe Leu Asn Gln Lys Leu Asn Thr Asp Thr Leu Ser Arg 190 105 110 Val Asn Ala Glu Leu Thr Gly Leu Gln Ala Asn Val Glu Glu Phe Asn 115 120 125 Arg Gln Val Asp Asn Phe Leu Asn Pro Asn Arg Asn Ala Val Pro Leu 130 135 140 Ser Ile Thr Ser Ser Val Asn Thr Met Gin Gln Leu Phe Leu Asn Arg 145 150 155 160 Leu Ser Gln Phe Gln Met Gln Gly Tyr Gln Leu Leu Leu Leu Pro Leu 165 170 175 Phe Ala Gln Ala Ala Asn Leu His Leu Ser Phe Ile Arg Asp Val Ile 180 185 190 Leu Asn Ala Glu Glu Trp Gly Ile Ser Ala Ala Thr Leu Arg Thr Tyr 195 200 205 Gln Asn His Leu Arg Asn Tyr Thr Arg Asp Tyr Ser Asn Tyr Cys Ile 210 215 220 Asp Thr Tyr Gln Thr Ala Phe Arg Gly Leu Asn Thr Arg Leu His Asp 225 230 235 240 Met Leu Glu Phe Arg Thr Tyr Met Phe Leu Asn Val Phe Glu Tyr Val 245 250 255 Ser Ile Trp Ser Leu Phe Lys Tyr Gln Ser Leu Leu Val Ser Ser Gly 260 265 270 Ala Asn Leu Tyr Ala Ser Gly Ser Gly Pro Gln Gln Thr Gln Leu Phe 275 280 285 Thr Ser Gln Asp Trp Pro Phe Leu Tyr Ser Leu Phe Gln Val Asn Ser 290 295 300 Asn Tyr Val Leu Ser Gly Phe Ser Gly Ala Ser Leu Phe Thr Thr Phe 305 310 315 320 Pro Asn Ile Gly Gly Leu Pro Gly Ser Thr Thr Thr Gln Ala Leu Leu 325 330 335 Ala Ala Arg Val Asn Tyr Ser Gly Gly Ile Thr Ser Gly Ser Ile Gly 340 345 350 Gly Ser Asn Phe Asn Gln Asn Phe Asn Cys Asn Thr Ile Ser Pro Pro 355 360 365 Leu Ser Thr Ser Phe Val Arg Ser Trp Leu Asp Ser Gly Ser Asp Arg 370 375 380 Gln Gly Val Asn Thr Val Thr Asn Trp Gln Thr Glu Ser Phe Glu Thr 385 390 395 400 Thr Ser Gly Leu Arg Cys Gly Ala Phe Thr Pro Arg Gly Asn Ser Asn 405 410 415 Tyr Tyr Pro Gly Tyr Phe Ile Arg Asn Ile Ser Gly Val Ser Leu Val 420 425 430
DK 181720 B1 Leu Arg Asn Glu Asp Leu Lys Arg Pro Leu Tyr Tyr Asn Glu Lys Arg 435 440 445 Asn Ile Glu Ser Pro Ser Gly Thr Pro Gly Gly Ala Arg Ala Tyr Met 450 455 460 Val Ser Val His Asn Lys Lys Asn Asn Ile Tyr Ala Val His Glu Asn 465 470 475 480 Gly Thr Met Ile His Leu Ala Pro Glu Asp Asn Thr Gly Phe Thr Ile 485 490 495 Ser Pro Ile His Ala Thr Gln Val Asn Asn Gln Thr Arg Thr Phe Ile 500 505 510 Ser Glu Lys Phe Gly Asn Gln Gly Asp Ser Leu Arg Phe Glu Gln Ser 515 520 525 Asn Thr Thr Ala Arg Tyr Thr Leu Arg Gly Asn Gly Asn Ser Tyr Asn 530 535 540 Leu Tyr Leu Arg Val Ser Ser Ile Gly Asn Ser Thr Ile Arg Val Thr 545 550 555 560 Ile Asn Gly Arg Val Tyr Thr Ala Ser Asn Val Asn Thr Thr Thr Asn 565 570 575 Asn Asp Gly Val Asn Asp Asn Gly Ala Arg Phe Ser Asp Ile Asn Ile 580 585 590 Gly Asn Val Val Ala Ser Ser Asn Ser Asp Val Pro Leu Asp Ile Asn 595 600 605 Val Thr Leu Asn Ser Gly Thr Gln Phe Asp Leu Met Asn Ile Met Leu 610 615 620 Val Pro Thr Asn Ile Ser Pro Leu Tyr Leu Glu 625 630 635 <210> 7 <211> 2004 <212> DNA <213> Bacillus thuringiensis <400> 7 catatgaatt catatcaaaa gaaaaatgat tacgaaatac tgaatgcttc accaagctat 60 tttaatacgt tggatagtta tccaagatat ccattagcaa atgatccaca agcttctatg 120 caaactagga attataaaga ttggcaaaat gaatgtgaag gaattactcc ttcaggattc 180 ggggcattta gctttataat atctattata atacaatttc aatcttttct ttcagagcct 240 tctttgtcag ggggattggg aataggattg gatatagctg acagcttaat agctgatgat 360 gtatcccaat tgtcagcgag ggaggtaaga gatcttattc aaagtgggct aacagactta 360 gctataagta tagcaagtgg gagttttaac gacatagtag agaaatatcg ggactatcta 420 gaggtgttta aaaggtggga aagcggagga aggacggatg acgataccag agagactatc 480 ggacgcctga ttatggatgc cgaaagggca gcagcagaac taaatcaatt ttcccacggt 540 gactataaga taccattatt gcctacctat gcacaagctg caaatcttca tttaaaaata 600 ttaagagata ttttgataca tggagaagat ttacaaataa taagcactgc tgatcgtcaa 660 ttctataggg ataaactatt tggtggtcta aaaaattata ctaattattg tgtagatcag 720 tataatgcag gtttaaacca gataaaagag gctggtacta gtgctgcaaa ttggctgaga 780 tttcacagat ttcgcaggga aatgacattg tcggtattag atttaattgc attatttcca 840 acttatgata ttgatagcta tgatttacca gtaaacgtgg aattatctag aataatttat 900 acagatccag taggttggac tgtagaaggg atgtcaacga tttacccttg gtatagtcct 960 tataataggc ttgcttttaa tcaattagaa gcagagacaa tacgtggecc gtatctctct 1020 acttggttgg attatatcga tatatattca tcacaatatt cctcattaaa agtgtggtct 1080 ggcaacgcca atacgatgaa gacgattggt ggcgaaacga gaagacaaga tggatctgca 1140 gtaggtactc ctcagcggat tactgtaggt aatactgata tttttaaagt agaactaact 1260 gctggtgttc accatttttt gattgattat actaatccta atccgcttcg ttcgtatgga 1260 attattaact ctaagttttt tatgacaaac ggatctactc cgacatatac agggtctgtt 1320 ggttcaatgc atactcaaac aggcaaagtt gcagctttac caggggtaga atcagaagtc 1380 ccaactgcaa atgattatac tcataggtta tctgatgtaa taaatttaac aggaggactt 1440 cctcaacatg gcagtaacag tgtacgctcc tccttagtag cacatggctg gacgcataaa 1500 agtttaaaac gtgaaaatat aattgaagcc aatcaaattt cacaaattcc tgcggtgaaa 1560 agttatagta tgagtggagg actaaccgtt aggcatgtct ccgatcatac aggtggtgac 1620 gtcgtttatt ttgctcaagt cggtagagta aagatattat gtaaatttac ttctccgggt 1680 tactataaaa tgcgtattag atatgcttca gattattcag ctacaatgac ggtcaatggc 1740 ataccggttt ctttatcatc cacaacacaa gttagatcaa atctgccgta tgaggcattt 1800 caatatgctc aagtcggtcc aaatcatgtt tttgctgttc ttacaccaaa aactctagaa 1860 tttgatattt atggtggagt caatggattc atgatagaca aaattgaatt tatcccaagt 1920 aatataacaa ctttagaata tgaggaagaa cgggacctag aaaaaacaaa aaacgcggtg 1980
DK 181720 B1 aacgatttat ttatcaatct cgag 2004 <210> 8 <211> 667 <212> PRT <213> Bacillus thuringiensis <400> 8 Met Asn Ser Tyr Gln Lys Lys Asn Asp Tyr Glu Ile Leu Asn Ala Ser 1510 15 Pro Ser Tyr Phe Asn Thr Leu Asp Ser Tyr Pro Arg Tyr Pro Leu Ala
Asn Asp Pro Gln Ala Ser Met Gln Thr Arg Asn Tyr Lys Asp Trp Gln
Asn Glu Cys Glu Gly Ile Thr Pro Ser Gly Phe Gly Ala Phe Ser Phe 60 Ile Ile Ser Ile Ile Ile Gln Phe Gln Ser Phe Leu Ser Glu Pro Ser 65 70 75 80 Leu Ser Gly Gly Leu Gly Ile Gly Leu Asp Ile Ala Asp Ser Leu Ile 85 90 95 Ala Asp Asp Val Ser Gln Leu Ser Ala Arg Glu Val Arg Asp Leu Ile 190 105 110 Gln Ser Gly Leu Thr Asp Leu Ala Ile Ser Ile Ala Ser Gly Ser Phe 115 120 125 Asn Asp Ile Val Glu Lys Tyr Arg Asp Tyr Leu Glu Val Phe Lys Arg 130 135 140 Trp Glu Ser Gly Gly Arg Thr Asp Asp Asp Thr Arg Glu Thr Ile Gly 145 150 155 160 Arg Leu Ile Met Asp Ala Glu Arg Ala Ala Ala Glu Leu Asn Gln Phe 165 170 175 Ser His Gly Asp Tyr Lys Ile Pro Leu Leu Pro Thr Tyr Ala Gln Ala 180 185 190 Ala Asn Leu His Leu Lys Ile Leu Arg Asp Ile Leu Ile His Gly Glu 195 200 205 Asp Leu Gln Ile Ile Ser Thr Ala Asp Arg Gln Phe Tyr Arg Asp Lys 210 215 220 Leu Phe Gly Gly Leu Lys Asn Tyr Thr Asn Tyr Cys Val Asp Gln Tyr 225 230 235 240 Asn Ala Gly Leu Asn Gln Ile Lys Glu Ala Gly Thr Ser Ala Ala Asn 245 250 255 Trp Leu Arg Phe His Arg Phe Arg Arg Glu Met Thr Leu Ser Val Leu 260 265 270 Asp Leu Ile Ala Leu Phe Pro Thr Tyr Asp Ile Asp Ser Tyr Asp Leu 275 280 285 Pro Val Asn Val Glu Leu Ser Arg Ile Ile Tyr Thr Asp Pro Val Gly 290 295 300 Trp Thr Val Glu Gly Met Ser Thr Ile Tyr Pro Trp Tyr Ser Pro Tyr 305 310 315 320 Asn Arg Leu Ala Phe Asn Gln Leu Glu Ala Glu Thr Ile Arg Gly Pro 325 330 335 Tyr Leu Ser Thr Trp Leu Asp Tyr Ile Asp Ile Tyr Ser Ser Gln Tyr 340 345 350 Ser Ser Leu Lys Val Trp Ser Gly Asn Ala Asn Thr Met Lys Thr Ile 355 360 365 Gly Gly Glu Thr Arg Arg Gln Asp Gly Ser Ala Val Gly Thr Pro Gin 370 375 380 Arg Ile Thr Val Gly Asn Thr Asp Ile Phe Lys Val Glu Leu Thr Ala 385 390 395 400 Gly Val His His Phe Leu Ile Asp Tyr Thr Asn Pro Asn Pro Leu Arg 405 410 415 Ser Tyr Gly Ile Ile Asn Ser Lys Phe Phe Met Thr Asn Gly Ser Thr 420 425 430 Pro Thr Tyr Thr Gly Ser Val Gly Ser Met His Thr Gln Thr Gly Lys 435 440 445 Val Ala Ala Leu Pro Gly Val Glu Ser Glu Val Pro Thr Ala Asn Asp 450 455 460
DK 181720 B1 Tyr Thr His Arg Leu Ser Asp Val Ile Asn Leu Thr Gly Gly Leu Pro 465 470 475 480 Gln His Gly Ser Asn Ser Val Arg Ser Ser Leu Val Ala His Gly Trp 485 490 495 Thr His Lys Ser Leu Lys Arg Glu Asn Ile Ile Glu Ala Asn Gln Ile 500 505 510 Ser Gln Ile Pro Ala Val Lys Ser Tyr Ser Met Ser Gly Gly Leu Thr 515 520 525 Val Arg His Val Ser Asp His Thr Gly Gly Asp Val Val Tyr Phe Ala 530 535 540 Gln Val Gly Arg Val Lys Ile Leu Cys Lys Phe Thr Ser Pro Gly Tyr 545 550 555 560 Tyr Lys Met Arg Ile Arg Tyr Ala Ser Asp Tyr Ser Ala Thr Met Thr 565 570 575 Val Asn Gly Ile Pro Val Ser Leu Ser Ser Thr Thr Gln Val Arg Ser 580 585 590 Asn Leu Pro Tyr Glu Ala Phe Gln Tyr Ala Gln Val Gly Pro Asn His 595 600 605 Val Phe Ala Val Leu Thr Pro Lys Thr Leu Glu Phe Asp Ile Tyr Gly 610 615 620 Gly Val Asn Gly Phe Met Ile Asp Lys Ile Glu Phe Ile Pro Ser Asn 625 630 635 640 Ile Thr Thr Leu Glu Tyr Glu Glu Glu Arg Asp Leu Glu Lys Thr Lys 645 650 655 Asn Ala Val Asn Asp Leu Phe Ile Asn Leu Glu 660 665
Application No. SEARCH REPORT - PATENT PA 2022 70092
1.U] Certain claims were found unsearchable (See Box No. D).
2. 1] Unity of invention is lacking prior to search (See Box No. ID.
A. CLASSIFICATION OF SUBJECT MATTER AOIN 63/23 (2020.01), AOIP 15/00 (2006.01) According to International Patent Classification (IPC)
B. FIELDS SEARCHED PCT-minimum documentation searched (classification system followed by classification symbols) IPC&CPC: AOIN, AO1P Documentation searched other than minimum documentation to the extent that such documents are included in the fields searched DK, NO, SE, FI: IPC-classes as above. Electronic database consulted during the search (name of database and, where practicable, search terms used) EPODOC, WPI, Search Report for WO 2021/028817 has been used, see this for details
C. DOCUMENTS CONSIDERED TO BE RELEVANT Category* Citation of document, with indication, where appropriate, of the relevant passages Relevant for claim No. A Regulation (EU) No 528/2012 concerning the making available on the market and 1-12 use of biocid-al products Evaluation of active substances - Assessment report: Bacillus thuringiensis kurstaki strain ABTS-351, 2016-02-01, pages 1 — 97 See pages 5-7 bips///echa suropa en/dommerts/ 10162/6a009719:480a-07 40.0050: 971 (10226007 A “Foray 48B Biological insecticide Flowable concentrate”, 2018-05-01, pages 1-27 1-12 See pages 4, 8-11 bips Av wd. epo. gov/pestindes/shem, seerety/ppls/073049.00427-20) 50501 pdf A WRIGHT, M. S. et al.: ”Mortality and repellent effects of microbial pathogens on 1-12 Coptotermes formosanus (Isoptera: Rhinotermitidae)”, BMC MICROBIOLOGY, BIOMED CENTRAL, Vol. 12, 2012-12-15, 291, pages 1-7 See page 2, right-hand column, last paragraph; page 4, table 2; page 5 10,1 186/1471-2180-12-291 DN] Further documents are listed in the continuation of Box C. * Special categories of cited documents: "rr Document published prior to the filing date but later than the "A" Document defining the general state of the art which is not priority date claimed. considered to be of particular relevance. T" Document not in conflict with the application but cited to "pr Document cited in the application. understand the principle or theory underlying the invention. "E" Farlier application or patent but published on or after the filing date. x Document of particular relevance; the claimed invention cannot be . Co . 0. considered novel or cannot be considered to involve an inventive "I" Document which may throw doubt on priority claim(s) or which is step when the document is taken alone cited to establish the publication date of another citation or other wom . to . . special reason (as specified). Y Document of particular relevance; the claimed invention cannot be . . considered to involve an inventive step when the document is "Oo" Document referring to an oral disclosure, use, exhibition or other combined with one or more other such documents. such means. combination being obvious to a person skilled in the art. "&" Document member of the same patent family. Danish Patent and Trademark Office Date of completion of the search report Helgeshøj Allé 81 06 February 2023 DK-2630 Taastrup Denmark Authorized officer Anita Thyrsted
Tel.: +45 4350 8000
Tel.: +45 43 50 81 32 October 2021 1/4
Application No. SEARCH REPORT - PATENT PA 2022 70092 C (Continuation). DOCUMENTS CONSIDERED TO BE RELEVANT Citation of document, with indication, where appropriate, of the relevant passages Relevant for claim No. A BRAVO, A. et al: “Evolution of Bacillus thuringiensis Cry toxins insecticidal 1-12 activity”, MICROBIAL BIOTECHNOLOGY, 2012-03-29, Vol. 6, pages 17 — 26 Cited in the application See abstract; page 18, left-hand column, last paragraph FOT IDEA A7D1: 7915 20 0200382 x A PALMA, L. et al.: “Bacillus thuringiensis Toxins: An Overview of Their Biocidal 1-12 Activity”, TOXINS, Vol. 6, 2014-12-11, pages 3296 — 3325 See abstract; page 3297; figure 1; page 3297, line 18 — page 3304, line 25
10.3390toxms61 23296 A US 2018/9127771 A1 (BONNING, B. C. et al.) 2018-05-10 1-12 See abstract; claim 1, Cryl Ab; SEQ ID NO: 2; paragraphs [0166], [0167] A IS 8735560 B1 (ENGLISH, L.H. et al.) 2014-05-27 1-12 See abstract; example 2, CrylAc; SEQ ID NO: 7 A WO 2099/052242 A2 (ATHENIX CORPORATION) 2009-04-23 1-12 Abstract; Cry2Adl; SEQ ID NO: 17 A WO 2013/134734 A2 (VESTARON CORPORATION) 2013-09-12 1-12 See abstract; Cry4; SEQ ID NO: 428 A MENDOZA-ESTRADA, L. J. ET AL.: “Anthelmintic Effect of Bacillus 1-12 thuringiensis Strains against the Gill Fish Trematode Centrocestus formosanus”, BIOMED RESEARCH INTERNATIONAL, Vol. 2016, 2016-05-17, pages 1 — 9 See abstract; page 2, left-hand column, line 37- right-hand column, line 17 1155/2016/8272407 A 402005154405 A (KYUSHU MEDICAL KK) 2005-06-16 1-12 Cited in the application See abstract; paragraphs [0001]-[0002] A OLMO, C. et al: “Effects of Bacillus thuringiensis var. israelensis on 1-12 nonstandard microcrustacean species isolated from field zooplankton communities”, ECOTOXICOLOGY, Vol. 25, 2016-09-17, pages 1730 — 1738 See abstract TO 1007/81 0640-016- 1708.9 A WO 2012/149549 A2 (AUBURN UNIVIVERSITY) 2012-11-01 1-12 Cited in the application See paragraphs [0001], [0003], |0004], [0008], [0018], [0025]-[0027], [0029],
[0071], [0072] X GB 2371053 A (BIOCONTROL LIMITED) 2002-07-17 1-12 See page 1, lines 3-24; page 4, lines 23-25; page 9, line 14-page 10, line 16; example 3; claims 1, 3, 5-7, 16 October 2021 2/4
Application No. SEARCH REPORT - PATENT PA 2022 70092 Box No. I Observations where certain claims were found unsearchable This search report has not been established in respect of certain claims for the following reasons:
1.0 Claims Nos.: because they relate to subject matter not required to be searched, namely:
2. Claims Nos.: because they relate to parts of the patent application that do not comply with the prescribed requirements to such an extent that no meaningful search can be carried out, specifically:
3. I] Claims Nos.: because of other matters. Box No. IX Observations where unity of invention is lacking prior to the search The Danish Patent and Trademark Office found multiple inventions in this patent application, as follows: October 2021 3/4
Application No.
SEARCH REPORT - PATENT
PA 2022 70092 SUPPLEMENTAL BOX Continuation of Box [.] October 2021 4/4
DKPA202270092A 2019-08-09 2022-03-08 Antiparasiticid formulering af bacillus thuringiensis-sporer, anvendelse af formuleringen til et veterinært medikament og et akvakulturelt parasiticidt kit DK181720B8 (da)

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