DK1935331T3 - Fremgangsmåde til fremstilling af modificerede elektroder, elektroder, der er fremstillet med denne fremgangsmåde, og enzymatiske biosensorer med disse elektroder - Google Patents
Fremgangsmåde til fremstilling af modificerede elektroder, elektroder, der er fremstillet med denne fremgangsmåde, og enzymatiske biosensorer med disse elektroder Download PDFInfo
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- DK1935331T3 DK1935331T3 DK07122884.5T DK07122884T DK1935331T3 DK 1935331 T3 DK1935331 T3 DK 1935331T3 DK 07122884 T DK07122884 T DK 07122884T DK 1935331 T3 DK1935331 T3 DK 1935331T3
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- 238000000034 method Methods 0.000 title claims description 67
- 230000008569 process Effects 0.000 title claims description 29
- 230000002255 enzymatic effect Effects 0.000 title claims description 15
- 238000004519 manufacturing process Methods 0.000 title description 4
- 239000000243 solution Substances 0.000 claims description 70
- DCYOBGZUOMKFPA-UHFFFAOYSA-N iron(2+);iron(3+);octadecacyanide Chemical compound [Fe+2].[Fe+2].[Fe+2].[Fe+3].[Fe+3].[Fe+3].[Fe+3].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] DCYOBGZUOMKFPA-UHFFFAOYSA-N 0.000 claims description 54
- 229960003351 prussian blue Drugs 0.000 claims description 53
- 239000013225 prussian blue Substances 0.000 claims description 53
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 claims description 18
- 230000008021 deposition Effects 0.000 claims description 15
- VTLYFUHAOXGGBS-UHFFFAOYSA-N Fe3+ Chemical compound [Fe+3] VTLYFUHAOXGGBS-UHFFFAOYSA-N 0.000 claims description 13
- 239000004094 surface-active agent Substances 0.000 claims description 11
- 239000012491 analyte Substances 0.000 claims description 10
- 239000013060 biological fluid Substances 0.000 claims description 10
- 150000002500 ions Chemical class 0.000 claims description 10
- -1 polyoxyethylene Chemical class 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 9
- 108090000790 Enzymes Proteins 0.000 claims description 8
- 102000004190 Enzymes Human genes 0.000 claims description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 8
- 229940088598 enzyme Drugs 0.000 claims description 8
- YAGKRVSRTSUGEY-UHFFFAOYSA-N ferricyanide Chemical compound [Fe+3].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] YAGKRVSRTSUGEY-UHFFFAOYSA-N 0.000 claims description 8
- 239000008103 glucose Substances 0.000 claims description 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 6
- 239000002253 acid Substances 0.000 claims description 6
- 229910002804 graphite Inorganic materials 0.000 claims description 5
- 239000010439 graphite Substances 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 230000015572 biosynthetic process Effects 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 3
- 150000007522 mineralic acids Chemical class 0.000 claims description 3
- 150000003839 salts Chemical class 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 claims description 2
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical group C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 2
- 229920003171 Poly (ethylene oxide) Chemical class 0.000 claims description 2
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical class [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims description 2
- 125000000129 anionic group Chemical group 0.000 claims description 2
- 229960000686 benzalkonium chloride Drugs 0.000 claims description 2
- CADWTSSKOVRVJC-UHFFFAOYSA-N benzyl(dimethyl)azanium;chloride Chemical class [Cl-].C[NH+](C)CC1=CC=CC=C1 CADWTSSKOVRVJC-UHFFFAOYSA-N 0.000 claims description 2
- 125000002091 cationic group Chemical group 0.000 claims description 2
- 229910017052 cobalt Inorganic materials 0.000 claims description 2
- 239000010941 cobalt Substances 0.000 claims description 2
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 claims description 2
- 235000014113 dietary fatty acids Nutrition 0.000 claims description 2
- 239000012153 distilled water Substances 0.000 claims description 2
- ICHUMRJJFKQBGK-UHFFFAOYSA-N dodecyl ethoxy sulfate Chemical class CCCCCCCCCCCCOS(=O)(=O)OOCC ICHUMRJJFKQBGK-UHFFFAOYSA-N 0.000 claims description 2
- 150000002148 esters Chemical class 0.000 claims description 2
- 229930195729 fatty acid Natural products 0.000 claims description 2
- 239000000194 fatty acid Substances 0.000 claims description 2
- 150000004665 fatty acids Chemical class 0.000 claims description 2
- UETZVSHORCDDTH-UHFFFAOYSA-N iron(2+);hexacyanide Chemical compound [Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] UETZVSHORCDDTH-UHFFFAOYSA-N 0.000 claims description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 2
- 229910052700 potassium Inorganic materials 0.000 claims description 2
- 239000011591 potassium Substances 0.000 claims description 2
- 229910052708 sodium Inorganic materials 0.000 claims description 2
- 239000011734 sodium Substances 0.000 claims description 2
- 235000019333 sodium laurylsulphate Nutrition 0.000 claims description 2
- 239000000600 sorbitol Substances 0.000 claims description 2
- 238000007650 screen-printing Methods 0.000 claims 4
- FBAFATDZDUQKNH-UHFFFAOYSA-M iron chloride Chemical compound [Cl-].[Fe] FBAFATDZDUQKNH-UHFFFAOYSA-M 0.000 claims 2
- 108010015776 Glucose oxidase Proteins 0.000 claims 1
- 239000002280 amphoteric surfactant Substances 0.000 claims 1
- 239000011260 aqueous acid Substances 0.000 claims 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims 1
- 229910000358 iron sulfate Inorganic materials 0.000 claims 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims 1
- MVFCKEFYUDZOCX-UHFFFAOYSA-N iron(2+);dinitrate Chemical compound [Fe+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O MVFCKEFYUDZOCX-UHFFFAOYSA-N 0.000 claims 1
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 17
- 238000000151 deposition Methods 0.000 description 16
- 238000002484 cyclic voltammetry Methods 0.000 description 10
- 238000002360 preparation method Methods 0.000 description 9
- 229910021578 Iron(III) chloride Inorganic materials 0.000 description 7
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 description 7
- 230000009467 reduction Effects 0.000 description 7
- 239000000976 ink Substances 0.000 description 6
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 6
- 230000008901 benefit Effects 0.000 description 5
- 230000003197 catalytic effect Effects 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 238000005234 chemical deposition Methods 0.000 description 3
- 235000019420 glucose oxidase Nutrition 0.000 description 3
- 229910052697 platinum Inorganic materials 0.000 description 3
- 229920000728 polyester Polymers 0.000 description 3
- 229920000136 polysorbate Polymers 0.000 description 3
- 229910052709 silver Inorganic materials 0.000 description 3
- 239000004332 silver Substances 0.000 description 3
- 238000001075 voltammogram Methods 0.000 description 3
- 238000004070 electrodeposition Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 239000011810 insulating material Substances 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- VCJMYUPGQJHHFU-UHFFFAOYSA-N iron(3+);trinitrate Chemical compound [Fe+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O VCJMYUPGQJHHFU-UHFFFAOYSA-N 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000002203 pretreatment Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- AWDBHOZBRXWRKS-UHFFFAOYSA-N tetrapotassium;iron(6+);hexacyanide Chemical compound [K+].[K+].[K+].[K+].[Fe+6].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] AWDBHOZBRXWRKS-UHFFFAOYSA-N 0.000 description 2
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 229920000557 Nafion® Polymers 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 229910008063 SnC Inorganic materials 0.000 description 1
- 239000004141 Sodium laurylsulphate Substances 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
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- 238000001035 drying Methods 0.000 description 1
- 238000002848 electrochemical method Methods 0.000 description 1
- 238000005868 electrolysis reaction Methods 0.000 description 1
- 229940073322 ferric hexacyanoferrate Drugs 0.000 description 1
- 229910021397 glassy carbon Inorganic materials 0.000 description 1
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- 229910052703 rhodium Inorganic materials 0.000 description 1
- 239000010948 rhodium Substances 0.000 description 1
- MHOVAHRLVXNVSD-UHFFFAOYSA-N rhodium atom Chemical compound [Rh] MHOVAHRLVXNVSD-UHFFFAOYSA-N 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
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- 229910021653 sulphate ion Inorganic materials 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/002—Electrode membranes
- C12Q1/003—Functionalisation
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- C23C18/00—Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating
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- C23C18/12—Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating by thermal decomposition characterised by the deposition of inorganic material other than metallic material
- C23C18/1204—Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating by thermal decomposition characterised by the deposition of inorganic material other than metallic material inorganic material, e.g. non-oxide and non-metallic such as sulfides, nitrides based compounds
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- C23C18/00—Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating
- C23C18/16—Chemical coating by decomposition of either liquid compounds or solutions of the coating forming compounds, without leaving reaction products of surface material in the coating; Contact plating by reduction or substitution, e.g. electroless plating
- C23C18/1601—Process or apparatus
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
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- G01N33/5438—Electrodes
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- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
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- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
- Y10T436/144444—Glucose
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Claims (23)
1. Fremgangsmåde til fremstilling af en serigrafi-elektrode, der er modificeret med preussiskblåt, kendetegnet ved, at den omfatter sekventiel afsætning på overfladen af serigrafi-elektroden af en opløsning, som omfatter jern-(lll)-ion eller jern-(ll)-ion og mindst ét overfladeaktivt middel i en passende opløsning, og en opløsning, som omfatter ferrocyanid-(ll)-ion eller ferricyanid-(lll)-ion og mindst ét overfladeaktivt middel i en passende opløsning, hvor opløsningerne har koncentrationer, som gør det muligt at opnå dannelsen af preussiskblåt direkte på overfladen af elektroden; hvor opløsningerne afsættes med ens volumen og ens koncentration; hvor den modificere elektrode derefter tørres i en ovn ved en temperatur på mellem 50°C og 200°C i en periode på mellem 10 minutter og 3 timer; hvor det overfladeaktive middel er udvalgt fra gruppen bestående af natriumlaurylsulphat og laurylethoxy-sulphat, benzalkoniumchlorid, polyoxyethylenderivativer af estere af fedtsyrer med sorbitol og blandinger deraf.
2. Fremgangsmåde ifølge krav 1, hvor serigrafi-elektroden ikke har gennemgået en forbehandling inden den sekventielle afsætning af opløsningerne.
3. Fremgangsmåde ifølge krav 1, hvor den opløsning, som omfatter jern-(l II)-ion eller jern-(ll)-ion, er en syreopløsning med en pFI-værdi på mellem 0,5 og 6.0 af en forbindelse fra gruppen bestående af jern-(lll)- eller jern-(ll)-salte af uorganisk syrer.
4. Fremgangsmåde ifølge krav 3, hvor jern-(lll)- eller jern-(ll)-saltene af uorganiske syrer er udvalgt fra jernchlorid, jernsulphat og jernnitrat.
5. Fremgangsmåde ifølge krav 3, hvor opløsningen er en opløsning af jernchlorid i vandig 0,01 M HCI med en pFI-værdi på 2,0.
6. Fremgangsmåde ifølge krav 1, hvor den opløsning, som omfatter hexacy-anoferrat (lll)-ion, er en syreopløsning med en pFI-værdi på mellem 0,5 og 6.0 af et salt, der indeholder ferrocyanid (II)- eller ferricyanid (lll)-ion.
7. Fremgangsmåde ifølge krav 6, hvor saltet er udvalgt fra natrium-, kalium-, ammonium- og cobalthexacyanoferrat.
8. Fremgangsmåde ifølge krav 6, hvor opløsningen er en opløsning af kali-umferricyanid K3Fe(CN)6 i vandig 0,01 M HCI med en pFI-værdi på 2,0.
9. Fremgangsmåde ifølge krav 1, hvor opløsningerne har en ens koncentration på mellem 20 mM og 2 mM, og er aflejret i ens volumen på mellem 100 nL og 4 μΙ_ til en elektrodeoverflade på mellem 0,314 mm2 og 3,14 cm2, fortrinsvis 3,14 mm2.
10. Fremgangsmåde ifølge krav 1, hvor serigrafi-elektroden er fremstillet af inert serigrafi-materiale med grafitblæk.
11. Fremgangsmåde ifølge krav 1, yderligere omfattende trin med at lade de aflejrede opløsninger hvile i 10 minutter, vaske overfladen af arbejdselektro-den med en vaskeopløsning, der består af en vandig syreopløsning med en pFI-værdi på mellem 0,5 og 6,0, vaske med destilleret vand, hvis det er nødvendig, og lade tørre i en ovn ved en temperatur mellem 50°C og 200°C, i en periode på mellem 10 minutter og 3 timer.
12. Fremgangsmåde ifølge krav 11, hvor vaskeopløsningen er en vandig opløsning af 0,01 M HCI med en pFI-værdi på 2,0.
13. Fremgangsmåde ifølge krav 12, hvor elektroden efterlades til at tørre i en ovn ved en temperatur på 100°C i en periode på 1 time og 30 minutter.
14. Fremgangsmåde ifølge krav 1, hvor det overfladeaktive middel er udvalgt fra kationiske, anioniske, amfoteriske overfladeaktive midler og blandinger deraf, og tilsat i mængder for hver opløsning på mellem 0,001 og 10 vægt% i forhold til den samlede volumen af opløsningen.
15. Fremgangsmåde ifølge krav 1, hvor det overfladeaktive middel er poly-oxyethylen-(20)-sorbitanmonolaurat, i en mængde på 0,05 vægt% i forhold til den samlede volumen af opløsningen.
16. Serigrafi-elektrode, der er modificeret med preussiskblåt, fremstillet med fremgangsmåden som defineret i kravene 1-15, hvor mængden af preussiskblåt er 100 nmol/cm2.
17. Enzymatisk elektrode, som omfatter den modificerede serigrafi-elektrode ifølge krav 16 og et egnet enzym, der er immobiliseret på elektroden og udvalgt på en sådan måde, at et produkt deraf kan oxideres eller reduceres elektrokemisk på den modificerede elektrode.
18. Enzymatisk elektrode ifølge krav 17, hvor enzymet er enzymglu-coseoxidase.
19. Enzymatisk biosensor, som omfatter den enzymatiske elektrode ifølge krav 17-18 som arbejdselektroden, en referenceelektrode, en modelektrode, hvis det er nødvendigt, og en celle til modtagelse af den biologiske fluid, der skal analyseres, således at sidstnævnte kan komme i kontakt med det enzym, der er immobiliseret på den enzymatiske elektrode.
20. Biosensor ifølge krav 19, omfattende serigrafi-elektroden og referenceelektroden som eneste elektroder.
21. Fremgangsmåde til vurdering af mængden af en analyt i en biologisk fluid omfattende påføring af en egnet potentiel værdi mellem en enzymatisk elektrode ifølge krav 17-18 og en referenceelektrode, som indeholdes i den enzymatiske biosensor ifølge krav 19-20, og læsning af det genererede strømsignal.
22. Fremgangsmåde ifølge krav 21, hvor analyten er glucose, og det påførte potentiale er mellem -250 og +200 mV.
23. Fremgangsmåde ifølge krav 22, hvor det påførte potentiale er lig med -50 mV.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT000322A ITFI20060322A1 (it) | 2006-12-13 | 2006-12-13 | Processo per la preparazione di elettrodi modificati, elettrodi preparati con tale processo, e biosensori enzimatici che li comprendono. |
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| DK1935331T3 true DK1935331T3 (da) | 2015-12-14 |
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| DK07122884.5T DK1935331T3 (da) | 2006-12-13 | 2007-12-11 | Fremgangsmåde til fremstilling af modificerede elektroder, elektroder, der er fremstillet med denne fremgangsmåde, og enzymatiske biosensorer med disse elektroder |
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| EP (1) | EP1935331B1 (da) |
| CY (1) | CY1117023T1 (da) |
| DK (1) | DK1935331T3 (da) |
| ES (1) | ES2555313T3 (da) |
| HR (1) | HRP20151244T1 (da) |
| HU (1) | HUE026692T2 (da) |
| IT (1) | ITFI20060322A1 (da) |
| ME (1) | ME02268B (da) |
| PL (1) | PL1935331T3 (da) |
| PT (1) | PT1935331E (da) |
| RS (1) | RS54399B1 (da) |
| RU (1) | RU2442130C2 (da) |
| SI (1) | SI1935331T1 (da) |
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| US7810380B2 (en) | 2003-03-25 | 2010-10-12 | Tearlab Research, Inc. | Systems and methods for collecting tear film and measuring tear film osmolarity |
| US7905134B2 (en) * | 2002-08-06 | 2011-03-15 | The Regents Of The University Of California | Biomarker normalization |
| GB0813200D0 (en) * | 2008-07-18 | 2008-08-27 | Univ Leeds | Hexacyanoferrate modified electrode |
| CN101825603A (zh) * | 2010-04-17 | 2010-09-08 | 上海交通大学 | 用于检测触酶阳性菌的电流型酶电极及其制备方法 |
| US9097661B2 (en) * | 2011-01-27 | 2015-08-04 | Caterpillar, Inc. | Electrochemical sulfur sensor and the method of making the same |
| RU2580288C2 (ru) * | 2013-04-29 | 2016-04-10 | Общество С Ограниченной Ответственностью "Русенс" | Способ изготовления микробиосенсора для определения глюкозы или лактата |
| CN104198554B (zh) * | 2014-09-03 | 2016-06-15 | 海南大学 | 一种工作电极及其制备方法、生物传感器 |
| BR112017005121A2 (pt) | 2014-09-23 | 2018-07-31 | Tearlab Res Inc | sistemas e métodos para integração de coleta de lágrima microfluídica e análise de fluxo lateral de analitos de interesse. |
| KR101633057B1 (ko) | 2015-04-22 | 2016-06-23 | 재단법인 실감교류인체감응솔루션연구단 | 헤드 마운트 디스플레이 시스템을 위한 얼굴 모션 캡쳐 방법 |
| TW201809655A (zh) * | 2016-06-01 | 2018-03-16 | 奧托德里克有限公司 | 用於測定生理樣本中陽離子濃度之普魯士藍網版印刷電極 |
| WO2017218197A1 (en) | 2016-06-15 | 2017-12-21 | Eastman Chemical Company | Physical vapor deposited biosensor components |
| WO2018052711A1 (en) | 2016-09-16 | 2018-03-22 | Eastman Chemical Company | Biosensor electrodes prepared by physical vapor deposition |
| CN109689880B (zh) | 2016-09-16 | 2022-12-13 | 伊士曼化工公司 | 通过物理气相沉积制备的生物传感器电极 |
| EP3642605B1 (en) | 2017-06-22 | 2026-04-15 | Eastman Chemical Company | Physical vapor deposited electrode for electrochemical sensors |
| RU2696499C1 (ru) * | 2018-02-08 | 2019-08-02 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Московский государственный университет имени М.В. Ломоносова" (МГУ) | Биосенсор для одновременного определения глюкозы и лактата в крови |
| RU2703316C1 (ru) * | 2018-11-29 | 2019-10-16 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Московский государственный университет имени М.В. Ломоносова" (МГУ) | Способ изготовления высокостабильного покрытия сенсора на пероксид водорода |
| US11331020B2 (en) | 2020-02-06 | 2022-05-17 | Trustees Of Boston University | Enzyme-based electrochemical nicotine biosensor |
| WO2021221752A2 (en) | 2020-02-06 | 2021-11-04 | Trustees Of Boston University | High throughput assay for identifying microbial redox enzymes |
| CN111272843B (zh) * | 2020-02-25 | 2023-01-06 | 衡阳师范学院 | 一种纳米线构建的FeCo网状结构纳米材料及其制备方法与应用 |
| CN112964772B (zh) * | 2021-03-30 | 2021-11-26 | 南京工业大学 | 一种基于丝网印刷芯片上酶的固定方法 |
| US11801000B2 (en) | 2021-04-30 | 2023-10-31 | Trustees Of Boston University | Hormone electrochemical biosensor |
| CN113945618B (zh) * | 2021-09-24 | 2024-02-09 | 合肥天一生物技术研究所有限责任公司 | 一种用于血浆中维生素b2含量检测的丝网印刷电极 |
| CN114062672B (zh) * | 2021-11-12 | 2023-04-25 | 福州大学 | 一种检测covid-19抗体的血糖生物传感器 |
| CN114965629B (zh) * | 2022-05-31 | 2023-07-25 | 浙江大学 | 一种乳酸生物传感器及其制备方法、一种细胞活力电化学检测方法 |
| CN115072740B (zh) * | 2022-06-23 | 2023-10-10 | 齐齐哈尔大学 | 一种核壳结构普鲁士蓝纳米球的制备方法 |
| CN115266865B (zh) * | 2022-07-29 | 2024-04-16 | 北京大学 | 一种提高电化学传感器稳定性的方法 |
| CN115494130A (zh) * | 2022-09-19 | 2022-12-20 | 北京大学 | 一种牙刷电化学传感器构建方法 |
| CN116429856A (zh) * | 2023-04-13 | 2023-07-14 | 苏州大学 | 一种基于三相酶电极的乳酸传感器及其制备方法 |
| CN117092185B (zh) * | 2023-08-22 | 2026-02-24 | 浙江大学滨江研究院 | 一种基于导电水凝胶-滕氏蓝纳米颗粒的复合电极及其制备方法和应用 |
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| JPS5858288A (ja) | 1981-10-02 | 1983-04-06 | Seiko Instr & Electronics Ltd | ヘキサシアノ鉄酸鉄の合成法 |
| US4652530A (en) * | 1985-07-15 | 1987-03-24 | Rohm And Haas Company | Monitoring method for isothiazolones in aqueous systems |
| EP0231476A1 (en) * | 1985-12-23 | 1987-08-12 | Siddiqi, Iqbal W., Dr. | Selectively ion-permeable electrodes for analyzing selected ions in aqueous solution |
| US5413690A (en) * | 1993-07-23 | 1995-05-09 | Boehringer Mannheim Corporation | Potentiometric biosensor and the method of its use |
| CN100405051C (zh) * | 2001-05-30 | 2008-07-23 | 爱-森斯株式会社 | 生物传感器 |
| KR100475634B1 (ko) * | 2001-12-24 | 2005-03-15 | 주식회사 아이센스 | 일정 소량의 시료를 빠르게 도입할 수 있는 시료도입부를구비한 바이오 센서 |
| US20060205090A1 (en) * | 2005-03-14 | 2006-09-14 | Newton Michael W | Water-soluble conjugates for electrochemical detection |
| US20100000882A1 (en) * | 2006-10-04 | 2010-01-07 | Arizona Board Of Regents For And On Behalf Of Arizona State University | Detection of Explosive Materials |
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2006
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- 2007-12-11 SI SI200731712T patent/SI1935331T1/sl unknown
- 2007-12-11 EP EP07122884.5A patent/EP1935331B1/en active Active
- 2007-12-11 RS RS20150762A patent/RS54399B1/sr unknown
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- 2007-12-11 ES ES07122884.5T patent/ES2555313T3/es active Active
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Also Published As
| Publication number | Publication date |
|---|---|
| CY1117023T1 (el) | 2017-04-05 |
| RS54399B1 (sr) | 2016-04-28 |
| ITFI20060322A1 (it) | 2008-06-14 |
| US20080160625A1 (en) | 2008-07-03 |
| EP1935331A2 (en) | 2008-06-25 |
| RU2442130C2 (ru) | 2012-02-10 |
| ES2555313T3 (es) | 2015-12-30 |
| HRP20151244T1 (hr) | 2016-01-29 |
| ME02268B (me) | 2016-04-28 |
| HUE026692T2 (en) | 2016-07-28 |
| US8309362B2 (en) | 2012-11-13 |
| PT1935331E (pt) | 2016-01-11 |
| EP1935331B1 (en) | 2015-09-09 |
| US20130001102A1 (en) | 2013-01-03 |
| SI1935331T1 (sl) | 2016-04-29 |
| RU2007146458A (ru) | 2009-06-20 |
| EP1935331A3 (en) | 2014-01-01 |
| PL1935331T3 (pl) | 2016-06-30 |
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