DK2013349T3 - Selektion af værtsceller, der udtrykker protein ved høje niveauer - Google Patents

Selektion af værtsceller, der udtrykker protein ved høje niveauer

Info

Publication number
DK2013349T3
DK2013349T3 DK07728440.4T DK07728440T DK2013349T3 DK 2013349 T3 DK2013349 T3 DK 2013349T3 DK 07728440 T DK07728440 T DK 07728440T DK 2013349 T3 DK2013349 T3 DK 2013349T3
Authority
DK
Denmark
Prior art keywords
polypeptide
interest
selectable marker
host cells
coding
Prior art date
Application number
DK07728440.4T
Other languages
English (en)
Inventor
Arie Pieter Otte
Blokland Henricus Johannes Maria Van
Theodorus Hendrikus Jacobus Kwaks
Richard George Antonius Bernardus Sewalt
Original Assignee
Chromagenics Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/416,490 external-priority patent/US20060195935A1/en
Application filed by Chromagenics Bv filed Critical Chromagenics Bv
Application granted granted Critical
Publication of DK2013349T3 publication Critical patent/DK2013349T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/40—Vector systems having a special element relevant for transcription being an insulator
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK07728440.4T 2006-05-02 2007-04-24 Selektion af værtsceller, der udtrykker protein ved høje niveauer DK2013349T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11/416,490 US20060195935A1 (en) 2004-11-08 2006-05-02 Selection of host cells expressing protein at high levels
EP06113354 2006-05-02
PCT/EP2007/053984 WO2007128685A1 (en) 2006-05-02 2007-04-24 Selection of host cells expressing protein at high levels

Publications (1)

Publication Number Publication Date
DK2013349T3 true DK2013349T3 (da) 2011-02-28

Family

ID=38324190

Family Applications (2)

Application Number Title Priority Date Filing Date
DK07712283.6T DK1987150T3 (da) 2006-02-21 2007-02-21 Selektion af værtsceller, der udtrykker protein på høje niveauer
DK07728440.4T DK2013349T3 (da) 2006-05-02 2007-04-24 Selektion af værtsceller, der udtrykker protein ved høje niveauer

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK07712283.6T DK1987150T3 (da) 2006-02-21 2007-02-21 Selektion af værtsceller, der udtrykker protein på høje niveauer

Country Status (13)

Country Link
EP (2) EP1987150B1 (da)
JP (1) JP5225107B2 (da)
KR (1) KR101328300B1 (da)
CN (2) CN101389763B (da)
AT (2) ATE511544T1 (da)
AU (1) AU2007247242B2 (da)
CA (2) CA2637271C (da)
DK (2) DK1987150T3 (da)
EA (1) EA014332B1 (da)
PL (1) PL1987150T3 (da)
PT (1) PT1987150E (da)
SI (1) SI1987150T1 (da)
WO (2) WO2007096399A2 (da)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE354645T1 (de) 2002-12-20 2007-03-15 Chromagenics Bv Mittel und methoden zur produktion eines proteins durch chromatin-öffner, die chromatin zugänglicher für transkriptionsfaktoren machen können
US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8039230B2 (en) 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
US7968700B2 (en) 2006-03-20 2011-06-28 Chromagenics B.V. Expression augmenting DNA fragments, use thereof, and methods for finding thereof
CN102325881B (zh) 2008-12-22 2014-08-20 国立大学法人北海道大学 用于用动物细胞大量生产源自外源基因的蛋白质的表达载体及其应用
JP5801207B2 (ja) 2009-02-27 2015-10-28 ノバルティス アーゲー ２種類の選択マーカーを含む発現ベクター系
US8765370B2 (en) * 2009-06-11 2014-07-01 Scinopharm Taiwan, Ltd Inhibition-based high-throughput screen strategy for cell clones
EP2443239B1 (en) 2009-06-15 2015-11-25 Cellagenics B.V. Novel stringent selectable markers
WO2011113841A1 (en) * 2010-03-16 2011-09-22 Robert Steinfeld Eukaryotic vector
JP5794541B2 (ja) * 2010-04-21 2015-10-14 国立大学法人北海道大学 核内移行性を有する脂質膜構造体
WO2011159157A1 (en) 2010-06-15 2011-12-22 Cellagenics B.V. Novel intergenic elements for enhancing gene expression
DK2611915T3 (da) 2010-09-01 2015-06-22 Cellagenics B V Nukleinsyrefragmenter fra en ribosomal proteinpromoter til forstærkning af genekspression
RU2716977C2 (ru) 2013-07-31 2020-03-17 Новартис Аг Новые селективные векторы и способы селекции эукариотических клеток-хозяев
CN104531699B (zh) * 2014-10-09 2017-03-15 河南农业大学 一种增强外源基因表达的猪的ucoe调控元件片段
CN106754817B (zh) * 2016-11-03 2021-02-05 山西省生物研究院有限公司 一种重组自分泌运动因子的表达方法
DE102017103383A1 (de) * 2017-02-20 2018-08-23 aReNA-Bio GbR (vertretungsberechtigter Gesellschafter: Dr. Heribert Bohlen, 50733 Köln) System und Verfahren zur Zelltyp-spezifischen Translation von RNA-Molekülen in Eukaryoten
TWI802728B (zh) * 2018-07-30 2023-05-21 大陸商南京金斯瑞生物科技有限公司 密碼子優化方法、包括其之系統及電子裝置、其核酸分子及使用其之蛋白質表現方法
WO2020036181A1 (ja) * 2018-08-13 2020-02-20 Ｓｐｉｂｅｒ株式会社 細胞を単離又は同定する方法及び細胞集団
CN110484563B (zh) * 2019-07-25 2023-04-07 新乡医学院 哺乳动物细胞组合表达载体、表达系统、制备方法和应用
KR102798616B1 (ko) 2019-12-20 2025-04-22 (주)셀트리온 목적 단백질의 고발현을 위한 인트론을 포함하는 발현 카세트 및 이의 이용

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5256568A (en) * 1990-02-12 1993-10-26 Regeneron Phamaceuticals, Inc. Vectors and transformed most cells for recombinant protein production with reduced expression of selectable markers
US6319707B1 (en) * 1991-08-12 2001-11-20 Fred Hutchinson Cancer Research Center Board Regents Of The University Of Washington Cap-independent multicistronic retroviral vectors
US5648267A (en) * 1992-11-13 1997-07-15 Idec Pharmaceuticals Corporation Impaired dominant selectable marker sequence and intronic insertion strategies for enhancement of expression of gene product and expression vector systems comprising same
WO1996012008A1 (en) * 1994-10-13 1996-04-25 Merck & Co., Inc. Synthesis of methylase-resistant genes
CA2489475C (en) * 2002-06-14 2011-03-29 Chromagenics B.V. A method for simultaneous production of multiple proteins; vectors and cells for use therein
US20060195935A1 (en) * 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
ES2384391T3 (es) * 2004-11-08 2012-07-04 Chromagenics B.V. Selección de células hospedantes que expresan proteína a altos niveles
KR101271884B1 (ko) * 2004-11-08 2013-06-05 크로마제닉스 비.브이. 고수준으로 단백질을 발현하는 숙주세포의 선택

Also Published As

Publication number Publication date
CN101389763A (zh) 2009-03-18
DK1987150T3 (da) 2011-09-19
JP5225107B2 (ja) 2013-07-03
EP2013349B1 (en) 2010-11-17
KR20080105034A (ko) 2008-12-03
CN101389763B (zh) 2013-01-23
EA200870279A1 (ru) 2009-02-27
PT1987150E (pt) 2011-09-08
ATE488593T1 (de) 2010-12-15
AU2007247242B2 (en) 2012-03-08
EA014332B1 (ru) 2010-10-29
WO2007128685A1 (en) 2007-11-15
EP1987150B1 (en) 2011-06-01
EP1987150A2 (en) 2008-11-05
EP2013349A1 (en) 2009-01-14
AU2007217431A1 (en) 2007-08-30
CA2651088C (en) 2016-03-08
JP2009527240A (ja) 2009-07-30
PL1987150T3 (pl) 2011-10-31
KR101328300B1 (ko) 2013-11-14
CA2637271C (en) 2014-12-09
CN101437946A (zh) 2009-05-20
WO2007096399A2 (en) 2007-08-30
HK1128490A1 (en) 2009-10-30
CN101437946B (zh) 2012-07-04
CA2637271A1 (en) 2007-08-30
WO2007096399A3 (en) 2008-05-02
SI1987150T1 (sl) 2011-09-30
ATE511544T1 (de) 2011-06-15
AU2007247242A1 (en) 2007-11-15
CA2651088A1 (en) 2007-11-15

Similar Documents

Publication Publication Date Title
DK2013349T3 (da) Selektion af værtsceller, der udtrykker protein ved høje niveauer
ZA200702046B (en) Selection of host cells expressing protein at high levels
Boussardon et al. Two interacting proteins are necessary for the editing of the NdhD-1 site in Arabidopsis plastids
NO20072845L (no) Seksjon av vertsceller som uttrykker hoye nivaer av proteiner
Hou et al. Molecular basis for the genome engagement by Sox proteins
Ricci et al. miRNA repression of translation in vitro takes place during 43S ribosomal scanning
Yamaguchi et al. VASCULAR‐RELATED NAC‐DOMAIN 7 directly regulates the expression of a broad range of genes for xylem vessel formation
de Tacca et al. PTBP1 mRNA isoforms and regulation of their translation
MX338911B (es) Identificacion mediada por trasposición de proteínas de unión o funcionales específicas.
EP1328653A4 (en) HIGHLY EXPRESSABLE GENES
WO2008005847A3 (en) Method of producing factor viii proteins by recombinant methods
Tzani et al. Tracing production instability in a clonally derived CHO cell line using single‐cell transcriptomics
BRPI0515264A (pt) construtores multiméricos
WO2008023247A2 (en) Matrix attachment regions (mars) for increasing transcription and uses thereof
Nomoto et al. Cloning‐free template DNA preparation for cell‐free protein synthesis via two‐step PCR using versatile primer designs with short 3′‐UTR
Ritter et al. Disruption of the gene C12orf35 leads to increased productivities in recombinant CHO cell lines
Bleckmann et al. Cytosolic RGG RNA-binding proteins are temperature sensitive flowering time regulators in Arabidopsis
Hang et al. HOT3/eIF5B1 confers Kozak motif-dependent translational control of photosynthesis-associated nuclear genes for chloroplast biogenesis
EP2325197A3 (en) Expression control elements from the Lemnaceae family
US20130157308A1 (en) Bidirectional promoter reporter vector for the analysis of dual regulatory elements
Baek et al. Unraveling productivity-enhancing genes in Chinese hamster ovary cells via CRISPR activation screening using recombinase-mediated cassette exchange system
WO2006085942A3 (en) Compositions and methods for regulating gene transcription
Polacek Atomic mutagenesis of the ribosome: towards a molecular understanding of translation
US11578335B2 (en) Synthetic algal promoters
CN100455665C (zh) 人食管癌细胞ezrin基因上游转录调控元件