DK201700251A1 - Cleaning compositions and uses thereof - Google Patents

Cleaning compositions and uses thereof Download PDF

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DK201700251A1
DK201700251A1 DKPA201700251A DKPA201700251A DK201700251A1 DK 201700251 A1 DK201700251 A1 DK 201700251A1 DK PA201700251 A DKPA201700251 A DK PA201700251A DK PA201700251 A DKPA201700251 A DK PA201700251A DK 201700251 A1 DK201700251 A1 DK 201700251A1
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Klaus Gori
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Novozymes As
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  • Detergent Compositions (AREA)
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Description

CLEANING COMPOSITIONS AND USES THEREOF Background
Enzymes have been used in detergents for decades. Usually a cocktail of various enzymes is added to detergent compositions. The enzyme cocktail often comprises various enzymes, wherein each enzyme targets it specific substrate e.g. amylases are active towards starch stains, proteases on protein stains and so forth. Textiles surface and hard surfaces, such as dishes or the inner space of a laundry machine enduring several wash cycles, become soiled with many different types of soiling which may compose of proteins, grease, starch etc. One type of soiling may be organic matter, such as biofilm, extracellular polymeric substance (EPS), etc. Organic matter composes different molecules such as polysaccharides, extracellular DNA (eDNA), and proteins. Some organic matter composes an extracellular polymeric matrix, which may be sticky or glueing, which when present on textile, attracts soils and may course redeposition or backstaining of soil resulting in a greying of the textile. Additionally, organic matters such as biofilms often cause malodor issue as various malodor molecules can be adhered by the polysaccharides, extracellular DNA (eDNA), and proteins in the complex extracellular matrix and be slowly released out to cause consumer noticeable malodor issue. There is a need for cleaning compositions, which effectively prevent, reduce or remove components of organic soiling.
Detailed Description
Various enzymes are applied in cleaning processes each targeting specific types of soiling such as protein, starch and grease soiling. Enzymes are now standard ingredients in detergents for laundry and dish wash. The effectiveness of these commercial enzymes provides detergents which removes much of the soiling. However, organic matters such as EPS (extracellular polymeric substance) comprised in much biofilm constitute a challenging type of soiling due to the complex nature of such organic matters. None of the commercially available cleaning compositions effectively remove or reduce EPS and/or biofilm related soiling. Biofilm may be produced when a group of microorganisms’ cells stick to each other or stick to a surface, such as a textile, dishware or hard surface or another kind of surface. These adherent cells are frequently embedded within a self-produced matrix of extracellular polymeric substance (EPS), which constitute 50% to 90% of the biofilm's total organic matter. EPS is mostly composed of polysaccharides (exopolysaccharides) and proteins, but include other macro-molecules such as eDNA, lipids and other organic substances. Organic matter like biofilm may be sticky or glueing, which when present on textile, may give rise to redeposition or backstaining of soil resulting in a greying of the textile. Another drawback of organic matter e.g. biofilm is the malodor as various malodor related molecules are often associated with organic matter e.g. biofilm. Further, when dirty laundry items are washed together with less dirty laundry items the dirt present in the wash liquor tend to stick to organic matter e.g. biofilm or biofilm components thus, hereof the laundry item is more “soiled” after wash than before wash. This is effect may also be termed re-deposition.
The composition is preferably a cleaning composition. The composition comprises at least one DNase and at least one secondary enzyme. Examples of useful DNases and secondary enzymes are mentioned below in the sections “Polypeptides having DNase activity” and “Secondary enzymes” respectively.
The compositions comprising a blend of DNase and secondary enzyme and are effective in reducing or removing organic components and soiling from organic matter. DNase enzymes
Polypeptides having DNase activity
The term “DNase” means a polypeptide with DNase activity that catalyzes the hydrolytic cleavage of phosphodiester linkages in a DNA backbone, thus degrading DNA. The term “DNases” and the expression “a polypeptide with DNase activity” are used interchangeably throughout. DNase activity may be determined according to the procedure described in the Assay I.
Preferably the DNase is selected from any of the enzyme classes E.C. 3.1.21 .X, where X = 1,2, 3, 4, 5, 6, 7, 8 or 9, e.g. Deoxyribonuclease I, Deoxyribonuclease IV, Type I site-specific deoxyribonuclease, Type II site-specific deoxyribonuclease, Type III site-specific deoxyribonuclease, CC-preferring endo-deoxyribonuclease, Deoxyribonuclease V, T(4) deoxyribonuclease II, T(4) deoxyribonuclease IV or E.C. 3.1.22.Y where Y = 1, 2, 4 or 5, e.g. Deoxyribonuclease II, Aspergillus deoxyribonuclease K(1), Crossover junction endo-deoxyribonuclease, Deoxyribonuclease X.
Preferably, the polypeptide having DNase activity is obtained from a microorganism and the DNase is a microbial enzyme. The DNase is preferably of fungal or bacterial origin.
The DNase may be obtainable from Bacillus e.g. Bacillus, such as a Bacillus licheniformis, Bacillus subtilis, Bacillus sp-62451, Bacillus horikoshii, Bacillus sp-62451, Bacillus sp-16840, Bacillus sp-62668, Bacillus sp-13395, Bacillus homeckiae, Bacillus sp-11238, Bacillus cibi, Bacillus idriensis, Bacillus sp-62520, Bacillus sp-16840, Bacillus sp-62668, Bacillus algicola, Bacillus vietnamensis, Bacillus hwajinpoensis, Bacillus indicus, Bacillus marisflavi, Bacillus luciferensis, Bacillus sp. SA2-6.
The DNase may also be obtained from any of the following Pyrenochaetopsis sp., Vibrissea flavovirens, Setosphaeria rostrate, Endophragmiella valdina, Corynespora cassiicola, Paraphoma sp. XZ1965, Monilinia fructicola, Curvularia lunata, Penicillium reticulisporum, Penicillium quercetorum, Setophaeosphaeria sp., Alternaria, Alternaria sp. XZ2545, Trichoderma reesei, Chaetomium thermophilum, Scytalidium thermophilum, Metapochonia suchlasporia, Daldinia fissa, Acremonium sp. XZ2007, Acremonium sp. XZ2414, Acremonium dichromosporum, Sarocladium sp. XZ2014, Metarhizium sp. HNA15-2, Isaria tenuipes
Scytalidium circinatum, Metarhizium lepidiotae, Thermobispora bispora, Sporormia fimetaria, Pycnidiophora cf. dispera, Enviromental sample D, Enviromental sample O, Clavicipitaceae sp-70249, Westerdykella sp. AS85-2, Humicolopsis cephalosporioides, Neosartorya massa, Roussoella intermedia, Pleosporales, Phaeosphaeria or Didymosphaeria futilis.
The DNases preferable belong to the NUC1 group of DNases. The NUC1 group of DNases comprises polypeptides which in addition to having DNase activity, may comprise one or more of the motifs [T/D/S][G/N]PQL, [F/L/Y/I]A[N/R]D[L/I/P/V], or C[D/N]T[A/R], One embodiment relates to a composition comprising polypeptides having DNase activity, wherein the polypeptides comprises one or more of the motifs [T/D/S][G/N]PQL, [F/L/Y/I]A[N/R]D[L/I/P/V] or C[D/N]T[A/R], The DNases preferably comprises a NUC1_A domain [D/Q][I/V]DH (SEQ ID NO 72). In addition to comprising any of the domains [T/D/S][G/N]PQL, [F/L/Y/I]A[N/R]D[L/I/P/V] or C[D/N]T[A/R] the polypeptides having DNase activity, belongs to the NUC1_A domain and may share the common motif [D/Q][I/V]DH (SEQ ID NO 72). One embodiment relates to compositions comprising polypeptides, which comprises one or more motifs selected from the motifs [E/D/H]H[I/V/L/F/M]X[P/A/S], [T/D/S][G/N]PQL, [F/L/Y/I]A[N/R]D[L/I/P/V], C[D/N]T[A/R] and [D/Q][I/V]DH, wherein the polypeptides have DNase activity. The DNases, preferably belong to the group of DNases comprised in the GYS-clade, which are NUC1 and NUC1A DNases further comprising the conservative motifs [D/M/L][S/T]GYSR[D/N] or ASXNRSKG and which share similar structural and functional properties. The DNases of the GYS-clade are preferably obtained from Bacillus genus.
One embodiment relates to a composition comprising a polypeptide of the GYS clade having DNase activity, optionally wherein the polypeptide comprises one or both motifs [D/M/L][S/T]GYSR[D/N], ASXNRSKG and wherein the polypeptide is selected from the group of polypeptides: a) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 1, b) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 2, c) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 3, d) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 4, e) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 5, f) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 6, g) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 7, h) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 8, i) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 9, j) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 10, k) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 11, l) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 12, m) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 13, n) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 14, o) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 15, p) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 16, q) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 17, r) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 18, s) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 19, t) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 20, u) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 21, v) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 22, w) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 23, x) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 24, and y) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 25.
Polypeptides having DNase activity and which comprise the GYS-clade motifs have shown particularly good deep cleaning properties e.g. the DNases are particularly effective in removing or reducing components of organic matter, such as biofilm, from an item such as a textile or a hard surface.
In one embodiment, the DNases preferably belong to the group of DNases comprised in the NAWK-clade, which are NUC1 and NUC1_A DNases further comprising the conservative motifs [V/I]PL[S/A]NAWK or NPQL.
One embodiment relates to a composition comprising a polypeptide of the NAWK-clade having DNase activity wherein the polypeptide comprises one or both motifs [V/I]PL[S/A]NAWK or NPQL and wherein the polypeptide is selected from the group of polypeptides: a) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 26, b) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 27, c) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 28, d) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 29, e) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 30, f) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 31, g) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 32, h) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 33, i) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 34, j) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 35, k) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 36, l) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 37, and m) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 38.
Polypeptides having DNase activity and which comprise the NAWK-clade motifs have shown particularly good deep cleaning properties e.g. the DNases are particularly effective in removing or reducing components of organic matter, such as biofilm, from an item such as a textile or a hard surface.
The DNases to be added in a composition preferably belong to the group of DNases comprised in the KNAW-clade, which are NUC1 and NUC1_A DNases further comprising the conservative motifs P[Q/E]L[W/Y] or [K/H/E]NAW.
One embodiment relates to a composition comprising a polypeptide of the KNAW clade having DNase activity wherein the polypeptide comprises one or both motifs P[Q/E]L[W/Y] or [K/H/E]NAW, and wherein the polypeptide is selected from the group of polypeptides: a) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 39, b) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 40, c) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 41, d) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 42, e) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 43 f) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 44, g) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 45, h) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 46, i) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 47, j) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 48, k) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 49, l) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 50, and m) a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 51.
Polypeptides having DNase activity and which comprise the KNAW-clade motifs have shown particularly good deep cleaning properties e.g. the DNases are particularly effective in removing or reducing components of organic matter, such as biofilm, from an item such as a textile or a hard surface.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 1 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1,2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 1.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 2 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1,2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 2.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 3 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1,2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 3.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 4 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1,2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 4.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 5 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 81%, at least 82%, at least 83%, at least 84%, at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1,2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 5.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 6 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 6.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 7 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 7.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 8 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 8.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 9 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 9.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 10 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 10.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 11 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 11.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 12 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 12.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 13 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 13.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 14 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 14.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 15 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 15.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 16 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 16.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 17 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 17.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 18 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 18.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 19 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 19.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 20 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 20.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 21 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 21.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 22 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 22.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 23 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 23.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 24 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 24.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 25 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 25.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 26 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 26.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 27 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 27.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 28 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 28.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 29 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 29.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 30 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 30.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 31 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 31.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 32 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 32.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 33 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 33.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 34 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 34.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 35 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 35.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 36 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 36.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 37 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 37.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 38 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 38.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 39 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 39.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 40 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 40.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 41 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 41.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 42 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 42.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 43 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 43.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 44 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 44.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 45 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 45.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 46 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 46.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 47 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 47.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 48 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 48.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 49 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 49.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 50 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 50.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 51 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 51.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 52 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 52.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 53 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 53.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 54 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 54.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 55 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 55.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 56 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 56.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 57 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 57.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 58 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 58.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 59 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 59.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 60 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 60.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 61 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 61.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 62 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 62.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 63 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 63.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 64 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 64.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 65 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1,2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 65.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 66 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1,2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 66.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 67 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1,2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 67.
In some embodiments, the composition comprises a polypeptide having a sequence identity to the polypeptide shown in SEQ ID NO: 68 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have DNase activity. In one aspect, the polypeptides differ by up to 10 amino acids, e.g., 1,2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 68.
The compositions comprising a blend of DNase and secondary enzyme and are effective in reducing or removing organic components and soiling from organic matter. Examples of secondary enzyme are listed below.
Secondary enzymes
Proteases
In one embodiment, the secondary enzyme is a protease. Protease activity may be determined as shown in Assay II. Suitable proteases include those of bacterial, fungal, plant, viral or animal origin e.g. vegetable or microbial origin. Microbial origin is preferred. Chemically modified or protein engineered mutants are included. It may be an alkaline protease, such as a serine protease or a metalloprotease. A serine protease may for example be of the S1 family, such as trypsin, or the S8 family such as subtilisin. A metalloproteases protease may for example be a thermolysin from e.g. family M4 or other metalloprotease such as those from M5, M7 or M8 families. The term "subtilases" refers to a sub-group of serine protease according to Siezen et al., Protein Engng. 4 (1991) 719-737 and Siezen et al. Protein Science 6 (1997) 501-523. Examples of subtilases are those derived from Bacillus such as Bacillus lentus, B. alkalophilus, B. subtilis, B. amyloliquefaciens, Bacillus pumilus and Bacillus gibsonii described in; US7262042 and W009/021867, and subtilisin lentus, subtilisin Novo, subtilisin Carlsberg, Bacillus licheniformis, subtilisin BPN’, subtilisin 309, subtilisin 147 and subtilisin 168 described in WO89/06279 and protease PD138 described in (WO93/18140). Other useful proteases may be those described in W092/175177, W001/016285, W002/026024 and W002/016547. Examples of trypsin-like proteases are trypsin (e.g. of porcine or bovine origin) and the Fusarium protease described in W089/06270, W094/25583 and W005/040372, and the chymotrypsin proteases derived from Cellumonas described in W005/052161 and W005/052146. A further preferred protease is the alkaline protease from Bacillus lentus DSM 5483, as described for example in W095/23221, and variants thereof which are described in W092/21760, W095/23221, EP1921147 and EP1921148.
Examples of metalloproteases are the neutral metalloprotease as described in WO07/044993 (Genencor Int.) such as those derived from Bacillus amyloliquefaciens. Suitable commercially available protease enzymes include those sold under the trade names Alcalase®, DuralaseTm, DurazyrnTm, Relase®, Relase® Ultra, Savinase®, Savinase® Ultra, Primase®, Polarzyme®, Kannase®, Liquanase®, Liquanase® Ultra, Ovozyme®, Coronase®, Coronase® Ultra, Blaze®, Blaze Evity® 100T, Blaze Evity® 125T, Blaze Evity® 150T, Neutrase®, Everlase® and Esperase® (Novozymes A/S), those sold under the tradename Maxatase®, Maxacal®, Maxapem®, Purafect Ox®, Purafect OxP®, Puramax®, FN2®, FN3®, FN4®, Excellase®, Excellenz P1000™, Excellenz P1250™, Eraser®, Preferenz P100™, Purafect Prime®, Preferenz P110™, Effectenz P1000™, Purafect®™, Effectenz P1050™, Purafect Ox®™, Effectenz P2000™, Purafast®, Properase®, Opticlean® and Optimase® (Danisco/DuPont), Axapem™ (Gist-Brocases N.V.), BLAP (sequence shown in Figure 29 of US5352604) and variants hereof (Henkel AG) and KAP (Bacillus alkalophilus subtilisin) from Kao.
In one embodiment, the protease is selected from a group consisting of: i) a protease variant of a protease parent, wherein the protease variant comprises one or more alteration(s) compared to a protease shown in SEQ ID NO 69 or SEQ ID NO 70 in one or more of the following positions: 3, 4, 9, 15, 24, 27, 42, 55, 59, 60, 66, 74, 85, 96, 97, 98, 99, 100, 101, 102, 104, 116, 118, 121, 126, 127, 128, 154, 156, 157, 158, 161, 164, 176, 179, 182, 185, 188, 189, 193, 198, 199, 200, 203, 206, 211, 212, 216, 218, 226, 229, 230, 239, 246, 255, 256, 268 and 269, wherein the positions correspond to the positions of the protease shown in SEQ ID NO 69 and wherein the protease variant has at least 80% sequence identity to SEQ ID NO 69, SEQ ID NO 70 or SEQ ID NO 71; ii) a protease variant of a protease parent, wherein the protease variant comprises one or more mutation selected from the group consisting of: S3T, V4I, S9R, S9E, A15T, S24G, S24R, K27R, N42R, S55P, G59E, G59D, N60D, N60E, V66A, N74D, N85S, N85R, G96S, G96A, S97G, S97D, S97A, S97SD, S99E, S99D, S99G, S99M, S99N, S99R, S99H, S101A, V1021, V102Y, V102N, S104A, G116V, G116R, H118D, H118N, N120S, S126L, P127Q, S128A, S154D, A156E, G157D, G157P, S158E, Y161A, R164S, Q176E, N179E, S182E, Q185N, A188P, G189E, V193M, N198D, V199I, Y203W, S206G, L211Q, L211D, N212D, N212S, M216S, A226V, K229L, Q230H, Q239R, N246K, N255W, N255D, N255E, L256E, L256D T268A and R269H, wherein the positions correspond to the positions of the protease shown in SEQ ID NO 69, wherein the protease variant has at least 80% sequence identity to SEQ ID NO 69, SEQ ID NO 70 or SEQ ID NO 71; iii) a protease comprising a substitution at one or more positions corresponding to positions 171, 173, 175, 179, or 180 of SEQ ID NO: 71, compared to the protease shown in SEQ ID NO 71, wherein the protease variant has a sequence identity of at least 75% but less than 100% to amino acid 1 to 311 of SEQ ID NO 71, iv) a protease comprising the amino acid sequence shown in SEQ ID NO 69, 70, 71 or a protease having at least 80% sequence identity to; the polypeptide comprising amino acids 1-269 of SEQ ID NO 69, the polypeptide comprising amino acids 1-311 of SEQ ID NO 71 or the polypeptide comprising amino acids 1-275 of SEQ ID NO 70; v) One or more of the following protease variants selected from the group: SEQ ID NO 69+ T22R+S99G+S101A+V102I+A226V+Q239R, SEQ ID NO 70+ S24G+S53G+S78N+S101N+G128A+Y217Q, SEQ ID NO 70+ S24G+S53G+S78N+S101N+G128S+Y217Q, SEQ ID NO 69+ S9E+ N42R+ N74D+ V199I+ Q200L+ Y203W+ S253D+ N255W+ L256E,
SEQ ID NO 69+ S9E+N42R+N74D+H118V+Q176E+A188P+V199I+Q200L Y203W+S250D+S253D+N255W+L256E
SEQ ID NO 69+ S9E+N42R+N74D+Q176E+A188P+V199I+Q200L+Y203W S250D+S253D+N255W+L256E
SEQ ID NO 69+ S3V+N74D+H118V+Q176E+N179E+S182E+V199I+Q200L Y203W+S210V+S250D+S253D+N255W+L256E
SEQ ID NO 69+ T22A+N60D+S99G+S101A+V102I+N114L+G157D +S182D+T207A+A226V+Q239R+N242D+E265F, SEQ ID NO 69+ S9E+N42R+ N74D+ H118V+Q176E+A188P+V199I+Q200L+ Y203W+ S250D+ S253D+ N255W+ L256E, SEQ ID NO 69+ S9E+N42R+ N74D+Q176E+A188P+V199I+Q200L+Y203W+ S250D+ S253D+ N255W+ L256E, SEQ ID NO 69+ S9E+ N42R+ N74D+ H118V+ Q176E+ A188P+V199I+ Q200L+ Y203W+ S250D+ N255W+ L256E+*269aH+ *269bH, SEQ ID NO 69+ S3V+ N74D+ H118V+ Q176E+ N179E+ S182E+ V199I+ Q200L+ Y203W+ S210V+ S250D+ N255W+ L256E, SEQ ID NO 69+ S9E+ N74D+ G113W+ G157P+ Q176E+ V199I+ Q200L+ Y203W+ S250D+ T254E+ N255W+ L256E, SEQ ID NO 69+ S3V+ S9R+ N74D+ H118V+ Q176E+ N179E+ S182E+ V199I+ Q200L+ Y203W+ S212V+ S250D+ N255W+ L256E, SEQ ID NO 69+S99E, and SEQ ID NO 70+L217D.
Wherein the SEQ ID NO XX + mutation(s) is a protease variants comprises the specified mutations compared to the parent sequence e.g. SEQ ID NO 70 + L217D is a protease variant of a protease shown in SEQ ID NO 70, which compared to SEQ ID NO 70 comprise the mutation L217D.
One embodiment relates to a composition comprising a) at least 0.00001 ppm of at least one polypeptide having DNase activity, wherein the DNase is selected for the group consisting of: i. a DNase comprising one or more of the motifs [T/D/S][G/N]PQL, [F/L/Y/I]A[N/R]D[L/I/P/V], or C[D/N]T[A/R]; ii. a DNase comprising the motif [D/Q][I/V]DH; iii. a DNase comprising one or both motifs [D/M/L][S/T]GYSR[D/N] or ASXNRSKG; iv. a DNase comprising one or both motifs [V/I]PL[S/A]NAWK or NPQL; v. a DNase comprising one or both motifs P[Q/E]L[W/Y] or [K/H/E]NAW; vi. a polypeptide having DNase activity selected from: a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 1, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 2, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 3, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 4, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 5, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 6, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 7, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 8, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 9, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 10, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 11, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 12, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 13, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 92, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 15, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 16, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 17, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 18, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 19, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 20, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 21, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 22, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 23, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 24, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 25, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 26, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 27, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 28, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 29, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 30, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 31, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 32, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 33, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 34, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 35, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 36, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 37, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 38, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 39, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 40, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 41, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 42, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 43, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 44, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 45, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 46, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 47, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 48, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 49, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 50, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 51, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 52, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 53, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 54, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 55, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 56, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 57, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 58, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 59, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 60, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 61, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 62, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 63, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 64, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 65, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 66, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 67, and a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 68, and b) at least 0.00001 ppm of one or more protease, wherein the protease is selected from, i) a protease variant of a protease parent, wherein the protease variant comprises one or more alteration(s) compared to a protease shown in SEQ ID NO 69 or SEQ ID NO 70 in one or more of the following positions: 3, 4, 9, 15, 24, 27, 42, 55, 59, 60, 66, 74, 85, 96, 97, 98, 99, 100, 101, 102, 104, 116, 118, 121, 126, 127, 128, 154, 156, 157, 158, 161, 164, 176, 179, 182, 185, 188, 189, 193, 198, 199, 200,203,206,211,212, 216, 218, 226, 229, 230, 239, 246, 255, 256, 268 and 269, wherein the positions correspond to the positions of the protease shown in SEQ ID NO 69 and wherein the protease variant has at least 80% sequence identity to SEQ ID NO 69, SEQ ID NO 70 or SEQ ID NO 71; ii) a protease variant of a protease parent, wherein the protease variant comprises one or more mutation selected from the group consisting of S3T, V4I, S9R, S9E, A15T, S24G, S24R, K27R, N42R, S55P, G59E, G59D, N60D, N60E, V66A, N74D, N85S, N85R, G96S, G96A, S97G, S97D, S97A, S97SD, S99E, S99D, S99G, S99M, S99N, S99R, S99H, S101A, V102I,V102Y,V102N,S104A,G116V,G116R, H118D, H118N, N120S, S126L, P127Q, S128A, S154D, A156E, G157D, G157P, S158E, Y161A, R164S, Q176E, N179E, S182E, Q185N, A188P, G189E, V193M, N198D, V199I, Y203W, S206G, L211Q, L211D, N212D, N212S, M216S, A226V, K229L, Q230H, Q239R, N246K, N255W, N255D, N255E, L256E, L256D T268A and R269H, wherein the positions correspond to the positions of the protease shown in SEQ ID NO 69, wherein the protease variant has at least 80% sequence identity to SEQ ID NO 69, SEQ ID NO 70 or SEQ ID NO 71; iii) a protease comprising a substitution at one or more positions corresponding to positions 171, 173, 175, 179, or 180 of SEQ ID NO 71, compared to the protease shown in SEQ ID NO 71, wherein the protease variant has a sequence identity of at least 75% but less than 100% to amino acid 1 to 311 of SEQ ID NO 71, iv) a protease comprising the amino acid sequence shown in SEQ ID NO 69, 80, 81, 82 or a protease having at least 80% sequence identity to; the polypeptide comprising amino acids 1-269 of SEQ ID NO 69, the polypeptide comprising amino acids 1-311 of SEQ ID NO 71, the polypeptide comprising amino acids 1-275 of SEQ ID NO 70, the polypeptide comprising amino acids 1-269 of SEQ ID NO 82; and v) one or more of the following protease variants selected from the group: SEQ ID NO 69+ T22R+S99G+S101A+V102I+A226V+Q239R; SEQ ID NO 70+ S24G+S53G+S78N+S101N+G128A+Y217Q; SEQ ID NO 70+ S24G+S53G+S78N+S101N+G128S+Y217Q; SEQ ID NO 69+ S9E+N42R+N74D+V199I+Q200L+Y203W+S253D+N255W+ L256E;
SEQ ID NO 69 +S9E+N42R+N74D+H118V+Q176E+A188P+V199I+Q200L +Y203W+S250D+ S253D+N255W+L256E;
SEQ ID NO 69 +S9E+N42R+N74D+Q176E+A188P+V199I+Q200L+Y203W S250D+S253D+N255W+L256E;
SEQ ID NO 69+ S3V+N74D+H118V+Q176E+N179E+S182E+V199I+Q200L Y203W+S210V+S250D+S253D+N255W+L256E; SEQ ID NO 69+ T22A+N60D+S99G+S101A+V102I+N114L+G157D+S182D+ T207A +A226V+Q239R+N242D+E265F; SEQ ID NO 69+ S9E+N42R+N74D+H118V+Q176E+ A188P+ V199I+ Q200L+ Y203W+ S250D+ S253D+ N255W+ L256E, SEQ ID NO 69+ S9E+ N42R+ N74D+ Q176E+ A188P+ V199I+ Q200L+ Y203W+ S250D+ S253D+ N255W+ L256E, SEQ ID NO 69+ S9E+ N42R+ N74D+ H118V+ Q176E+ A188P+ V199I+ Q200L+ Y203W+ S250D+ N255W+ L256E+ *269aH+ *269bH, SEQ ID NO 69+ S3V+ N74D+ H118V+ Q176E+ N179E+ S182E+ V199I+ Q200L+ Y203W+ S210V+ S250D+ N255W+ L256E, SEQ ID NO 69+ S9E+ N74D+ G113W+ G157P+ Q176E+ V199I+ Q200L+ Y203W+ S250D+ T254E+ N255W+ L256E, SEQ ID NO 69+ S3V+ S9R+ N74D+ H118V+ Q176E+ N179E+ S182E+ V199I+ Q200L+ Y203W+ S212V+ S250D+ N255W+ L256E, SEQ ID NO 69+ S99E, SEQ ID NO 70+ L217D and c) At least one cleaning component, preferably selected from surfactants, builders, bleach components, polymers, dispersing agents and additional enzymes.
Carbohvdrases
In one embodiment, the secondary enzyme is a carbohydrase. Suitable carbohydrases include amylases, cellulases and mannanases. The carbohydrases to be incorporated in a composition are preferably selected from cellulases, mannanases, and amylases.
Mannanases
In one embodiment, the secondary enzyme is a mannanase. A mannanase is an enzyme catalyzing hydrolysis of mannan. Mannanase activity may be determined as described in the Assay III.
Suitable mannanases include those of bacterial or fungal origin. Chemically or genetically modified mannanases are included. The mannanase may be an alkaline mannanase of Family 5 or 26. It may be a wild-type from Bacillus or Humicola, particularly B. agaradhaerens, B. licheniformis, B. halodurans, B. clausii, or H. insolens. Suitable mannanases are described in WO 1999/064619. Commercially available mannanases are Mannaway (Novozymes NS), and EFFECTENZ™ M1000 from Dupont. In one embodiment, the mannanases include polypeptides having a sequence identity to the polypeptide shown in SEQ ID NO: 72 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have mannanase activity. In one embodiment, the polypeptides differ by up to 10 amino acids, e.g., 1,2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO: 72.
Cellulases
In one embodiment, the secondary enzyme is a cellulase. A cellulase is any enzyme that hydrolyses cellulose. In a preferred embodiment, the cellulase is an endoglucanase. Cellulase activity is determined, as shown in Assay V. Suitable cellulases include those of bacterial or fungal origin. Chemically modified or protein engineered mutants are included. Suitable cellulases include cellulases from the genera Bacillus, Pseudomonas, Humicola, Fusarium, Thielavia, Acremonium, e.g., the fungal cellulases produced from Humicola insolens, Myceliophthora thermophila and Fusarium oxysporum disclosed in US 4,435,307, US 5,648,263, US 5,691,178, US 5,776,757 and WO 89/09259. Especially suitable cellulases are the alkaline or neutral cellulases having color care benefits. Examples of such cellulases are cellulases described in EP 0 495 257, EP 0 531 372, WO 96/11262, WO 96/29397, WO 98/08940. Other examples are cellulase variants such as those described in WO 94/07998, EP 0 531 315, US 5,457,046, US 5,686,593, US 5,763,254, WO 95/24471, WO 98/12307 and W099/001544. Other cellulases are endo-beta-1,4-glucanase enzyme having a sequence of at least 97% identity to the amino acid sequence of position 1 to position 773 of SEQ ID NO:2 of WO 2002/099091 ora family 44 xyloglucanase, which a xyloglucanase enzyme having a sequence of at least 60% identity to positions 40-559 of SEQ ID NO: 2 of WO 2001/062903.
Commercially available cellulases include Celluzyme™, and Carezyme™ (Novozymes A/S) Carezyme Premium™ (Novozymes A/S), Celluclean ™ (Novozymes NS), Celluclean Classic™ (Novozymes A/S), Cellusoft™ (Novozymes A/S), Whitezyme™ (Novozymes A/S), Clazinase™, and Puradax HA™ (Genencor International Inc.), and KAC-500(B)™ (Kao Corporation), Revitalenz™ 1000, Revitalenz™ 2000, Revitalenz™ 3000 (Dupont).
In one embodiment, the cellulases include polypeptides having a sequence identity to the polypeptide shown in SEQ ID NO 73 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have cellulase activity. In one embodiment, the polypeptides differ by up to 10 amino acids, e.g., 1,2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO 73.
In one embodiment, the cellulases include polypeptides having a sequence identity to the polypeptide shown in SEQ ID NO 74 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have cellulase activity. In one embodiment, the polypeptides differ by up to 10 amino acids, e.g., 1,2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO 74.
In one embodiment, the cellulases include polypeptides having a sequence identity to the polypeptide shown in SEQ ID NO 75 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have cellulase activity. In one embodiment, the polypeptides differ by up to 10 amino acids, e.g., 1,2,3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO 75.
In one embodiment, the cellulases include polypeptides having a sequence identity to the polypeptide shown in SEQ ID NO 76: of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have cellulase activity. In one embodiment, the polypeptides differ by up to 10 amino acids, e.g., 1,2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO 76.
In one embodiment, the cleaning composition comprise a xyloglucanase. A “xyloglucanase activity” is an enzyme that catalyze hydrolysis of xyloglucan, which is shown in Assay IV. Xyloglucanase can comprise parent xyloglucanase and the variants thereof.
In one embodiment, the xyloglucanase include polypeptides having a sequence identity to the polypeptide shown in SEQ ID NO 77 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have xyloglucanase activity. In one embodiment, the polypeptides differ by up to 10 amino acids, e.g., 1,2, 3, 4,5,6,7,8,9, or 10, from the mature polypeptide shown in SEQ ID NO 77.
Amylases
In one embodiment, the secondary enzyme is an amylase. An amylase is an enzyme that hydrolyze starch into sugars, amylase activity is determined as shown in Assay VI. Suitable amylases include alpha-amylases and/or a glucoamylase and may be of bacterial orfungal origin. Chemically modified or protein engineered mutants are included. Amylases include, for example, alpha-amylases obtained from Bacillus, e.g., a special strain of Bacillus licheniformis, described in more detail in GB 1,296,839. Additional amylases include amylases having SEQ ID NO: 2 in WO 95/10603 or variants having 90% sequence identity to SEQ ID NO: 3 thereof. Preferred variants are described in WO 94/02597, WO 94/18314, WO 97/43424 and SEQ ID NO: 4 of WO 99/019467, such as variants with substitutions in one or more of the following positions: 15, 23, 105, 106, 124, 128, 133, 154, 156, 178, 179, 181, 188, 190, 197,201,202, 207, 208,209,211, 243,264, 304, 305, 391,408, and 444. Different suitable amylases include amylases having SEQ ID NO: 6 in WO 02/010355 or variants thereof having 90% sequence identity to SEQ ID NO: 6. Preferred variants of SEQ ID NO: 6 are those having a deletion in positions 181 and 182 and a substitution in position 193. Other amylases which are suitable are hybrid alpha-amylase comprising residues 1-33 of the alpha-amylase derived from B. amyloliquefaciens shown in SEQ ID NO: 6 of WO 2006/066594 and residues 36-483 of the B. licheniformis alpha-amylase shown in SEQ ID NO: 4 of WO 2006/066594 or variants having 90% sequence identity thereof. Preferred variants of this hybrid alpha-amylase are those having a substitution, a deletion or an insertion in one of more of the following positions: 48, 49, 107, 156, 181, 190, 197, 201, 209 and 264. Most preferred variants of the hybrid alpha-amylase comprising residues 1-33 of the alpha-amylase derived from B. amyloliquefaciens shown in SEQ ID NO: 6 of WO 2006/066594 and residues 36-483 of SEQ ID NO: 4 are those having the substitutions: M197T; H156Y+A181T+N190F+A209V+Q264S or G48A+T49I+G107A+H156Y+A181T+N190F +1201F+A209V+Q264S. Further amylases which are suitable are amylases having SEQ ID NO: 6 in WO 99/019467 or variants thereof having 90% sequence identity to SEQ ID NO: 6. Preferred variants of SEQ ID NO: 6 are those having a substitution, a deletion or an insertion in one or more of the following positions: R181, G182, H183, G184, N195, I206, E212, E216and K269. Particularly preferred amylases are those having deletion in positions R181 and G182, or positions H183 and G184. Additional amylases which can be used are those having SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 2 or SEQ ID NO: 7 of WO 96/023873 or variants thereof having 90% sequence identity to SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 or SEQ ID NO: 7. Preferred variants of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 or SEQ ID NO: 7 are those having a substitution, a deletion or an insertion in one or more of the following positions: 140, 181, 182,183, 184,195, 206, 212, 243, 260, 269,304 and 476, using SEQ ID 2 of WO 96/023873 for numbering. More preferred variants are those having a deletion in two positions selected from 181, 182, 183 and 184, such as 181 and 182, 182 and 183, or positions 183 and 184. Most preferred amylase variants of SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 7 are those having a deletion in positions 183 and 184 and a substitution in one or more of positions 140, 195, 206, 243, 260, 304 and 476. Other amylases which can be used are amylases having SEQ ID NO: 2 of WO 08/153815, SEQ ID NO: 10 in WO 01/66712 or variants thereof having 90% sequence identity to SEQ ID NO: 2 of WO 08/153815 or 90% sequence identity to SEQ ID NO: 10 in WO 01/66712. Preferred variants of SEQ ID NO: 10 in WO 01/66712 are those having a substitution, a deletion or an insertion in one of more of the following positions: 176,177, 178, 179,190, 201, 207, 211 and 264. Further suitable amylases are amylases having SEQ ID NO: 2 of WO 09/061380 or variants having 90% sequence identity to SEQ ID NO: 2 thereof. Preferred variants of SEQ ID NO: 2 are those having a truncation of the C-terminus and/or a substitution, a deletion or an insertion in one of more of the following positions: Q87, Q98, S125, N128, T131, T165, K178, R180, S181, T182, G183, M201, F202, N225, S243, N272, N282, Y305, R309, D319, Q320, Q359, K444 and G475. More preferred variants of SEQ ID NO: 2 are those having the substitution in one of more of the following positions: Q87E,R, Q98R, S125A, N128C, T131I, T165I, K178L, T182G, M201L, F202Y, N225E,R, N272E,R, S243Q,A,E,D, Y305R, R309A, Q320R, Q359E, K444E and G475K and/or deletion in position R180 and/or S181 or of T182 and/or G183. Most preferred amylase variants of SEQ ID NO: 2 are those having the substitutions:N128C+K178L+T182G+Y305R+G475K; N128C+K178L+T182G+F202Y+Y305R+D319T+G475K; S125A+N128C+K178L+T182G+Y305R+G475K;or S125A+N128C+T131I+T165I+K178L+T182G+Y305R+G475K, wherein the variants are C-terminally truncated and optionally further comprises a substitution at position 243 and/or a deletion at position 180 and/or position 181. Further suitable amylases are amylases having SEQ ID NO: 1 of W013184577 or variants having 90% sequence identity to SEQ ID NO: 1 thereof. Preferred variants of SEQ ID NO: 1 are those having a substitution, a deletion or an insertion in one of more of the following positions: K176, R178, G179, T180, G181, E187, N192, M199, I203, S241, R458, T459, D460, G476 and G477. More preferred variants of SEQ ID NO: 1 are those having the substitution in one of more of the following positions: K176L, E187P, N192FYH, M199L, I203YF, S241QADN, R458N, T459S, D460T, G476K and G477K and/or deletion in position R178 and/or S179 or of T180 and/or G181. Most preferred amylase variants of SEQ ID NO: 1 are those having the substitutions:
E187P+I203Y+G476K E187P+I203Y+R458N+T459S+D460T+G476K, wherein the variants optionally further comprise a substitution at position 241 and/or a deletion at position 178 and/or position 179.
Further suitable amylases are amylases having SEQ ID NO: 1 of W010104675 or variants having 90% sequence identity to SEQ ID NO: 1 thereof. Preferred variants of SEQ ID NO: 1 are those having a substitution, a deletion or an insertion in one of more of the following positions: N21, D97, V128 K177, R179, S180, 1181, G182, M200, L204, E242, G477 and G478. More preferred variants of SEQ ID NO: 1 are those having the substitution in one of more of the following positions: N21D, D97N, V128I K177L, M200L, L204YF, E242QA, G477K and G478K and/or deletion in position R179 and/or S180 or of 1181 and/or G182. Most preferred amylase variants of SEQ ID NO: 1 are those having the substitutions: N21D+D97N+V128I, wherein the variants optionally further comprise a substitution at position 200 and/or a deletion at position 180 and/or position 181. Other suitable amylases are the alpha-amylase having SEQ ID NO: 12 in WO01/66712 or a variant having at least 90% sequence identity to SEQ ID NO: 12. Preferred amylase variants are those having a substitution, a deletion or an insertion in one of more of the following positions of SEQ ID NO: 12 in WO01/66712: R28, R118, N174; R181, G182, D183, G184, G186, W189, N195, M202, Y298, N299, K302, S303, N306, R310, N314; R320, H324, E345, Y396, R400, W439, R444, N445, K446, Q449, R458, N471, N484. Particular preferred amylases include variants having a deletion of D183 and G184 and having the substitutions R118K, N195F, R320K and R458K, and a variant additionally having substitutions in one or more position selected from the group: M9, G149, G182, G186, M202, T257, Y295, N299, M323, E345 and A339, most preferred a variant that additionally has substitutions in all these positions. Other examples are amylase variants such as those described in WO2011/098531, WO2013/001078 and WO2013/001087. Commercially available amylases are Duramyl™, Termamyl™, Fungamyl™, Stainzyme ™, Stainzyme Plus™, Natalase™, Liquozyme X and BAN™ (from Novozymes NS), and Rapidase™, Purastar™/Effectenz™, Powerase, Preferenz S1000, Preferenz S100 and Preferenz S110 (from Genencor International Inc./DuPont).
Further suitable amylases are amylases having SEQ ID NO: 1 or SEQ ID NO: 14 of WO 2016/203064 or variants having at least 75% identity SEQ ID NO: 1 or 14 thereof. Preferred variants of either SEQ ID NO: 1 or SEQ ID NO: 14 are those having a modification in one or more positions corresponding to positions 1, 54, 56, 72, 109, 113, 116, 134, 140, 159, 167, 169, 172, 173, 174, 181, 182, 183, 184, 189, 194, 195, 206, 255, 260, 262, 265, 284, 289, 304, 305, 347, 391,395, 439, 469, 444, 473, 476, or 477 of SEQ ID NO: 1. More preferred variants of SEQ ID NO: 1 or 14 are those having one or more of the following modifications: X1*, X1A, X54S, X56T, X72R, X109A, X113Q, X116Q, X116H, X134E, X140Y, X140F, X140H, X159Y, X159F, X159H, X167Y, X167H, X167F, X169E, X172K, X172G, X172N, X173P, X174*, X174S, X181*, X182*, X183*, X184*, X184T, X189Y, X189F, X189H, X189E, X189D, X189Q, X189N, X194D, X194N, X194S, X195F, X206L, X206F, X206Y, X255A, X260G, X260P, X260A, X260G, X260P, X260A, X265G, X284G, X284H, X289H, X304K, X304R, X304Q, X304E, X305K, X305R, X305Q, X305E, X347Y, X347F, X347H, X391A, X395P, X439N, X439Q, X439T, X444Q, X469T, X469N, X473R, X476R, X476Q, X476E, X476K, X477K, X477R, X477Q, and X477E wherein the positions correspond to positions of SEQ ID NO: 1 thereof.
In one embodiment, the amylase includes polypeptides having a sequence identity to the polypeptide shown in SEQ ID NO 78 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have amylase activity. In one embodiment, the polypeptides differ by up to 10 amino acids, e.g., 1,2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO 78.
In one embodiment, the amylase includes polypeptides having a sequence identity to the polypeptide shown in SEQ ID NO 79 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have amylase activity. In one embodiment, the polypeptides differ by up to 10 amino acids, e.g., 1,2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO 79.
In one embodiment, the amylase includes polypeptides having a sequence identity to the polypeptide shown in SEQ ID NO 80 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have amylase activity. In one embodiment, the polypeptides differ by up to 10 amino acids, e.g., 1,2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO 80.
In one embodiment, the amylase includes polypeptides having a sequence identity to the polypeptide shown in SEQ ID NO 81 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have amylase activity. In one embodiment, the polypeptides differ by up to 10 amino acids, e.g., 1,2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO 81.
In one embodiment, the amylase includes polypeptides having a sequence identity to the polypeptide shown in SEQ ID NO 83 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have amylase activity. In one embodiment, the polypeptides differ by up to 10 amino acids, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO 83.
In one embodiment, the amylase includes polypeptides having a sequence identity to the polypeptide shown in SEQ ID NO 84 of at least 60%, e.g., at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% and which have amylase activity. In one embodiment, the polypeptides differ by up to 10 amino acids, e.g., 1,2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide shown in SEQ ID NO 84.
One embodiment relates to a composition comprising a) at least 0.00001 ppm of at least one polypeptide having DNase activity, wherein the DNase is selected for the group consisting of: i) a DNase comprising one or more of the motif(s) [T/D/S][G/N]PQL, [F/L/Y/l]A[N/R]D[L/l/P/V], C[D/N]T[A/R]; ii) a DNase comprising the motif [D/Q][I/V]DH; iii) a DNase comprising one or both motif(s) [D/M/L][S/T]GYSR[D/N] or ASXNRSKG; iv) a DNase comprising one or both motifs [V/I]PL[S/A]NAWK or NPQL; v) a DNase comprising one or both motifs P[Q/E]L[W/Y] or [K/H/E]NAW; vi) a DNase selected from: a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 1, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 2, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 3, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 4, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 5, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 6, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 7, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 8, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 9, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 10, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 11, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 12, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 13, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 14, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 15, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 16, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 17, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 18, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 19, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 20, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 21, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 22, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 23, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 24, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 25, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 26, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 27, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 28, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 29, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 30, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 31, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 32, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 33, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 34, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 35, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 36, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 37, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 38, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 39, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 40, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 41, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 42, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 43, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 44, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 45, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 46, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 47, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 48, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 49, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 50, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 51, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 52, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 53, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 54, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 55, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 56, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 57, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 58, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 59, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 60, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 61, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 62, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 63, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 64, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 65, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 66, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 67, and a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 68; and b) at least 0.00001 ppm of one or more carbohydrase, wherein the carbohydrase is selected from the group consisting of; i. a cellulase selected from a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 73, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 74, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 75, and a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 76; ii. a xylanase selected from a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 77; iii. a mannanase selected from a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 72; iv. an amylase selected from a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 78, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 79, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 80, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 81; a polypeptide having at least 75% sequence identity to the polypeptide shown in SEQ ID NO: 83; and a polypeptide having at least 75% sequence identity to the polypeptide shown in SEQ ID NO: 84; and c) at least one cleaning component, preferably selected from surfactants, builders, bleach components, polymers and dispersing agents.
Haloperoxidase
In one embodiment, the secondary enzyme is a haloperoxidase. Suitable haloperoxidases include chloroperoxidases and bromoperoxidases compounds exhibiting chloroperoxidase or bromoperoxidase activity. Haloperoxidases form a class of enzymes that are capable of oxidizing halides (Cl-, Br-, I-) in the presence of hydrogen peroxide or a hydrogen peroxide generating system to the corresponding hypohalous acids or hypohalites. Haloperoxidase activity is determined as described in the Assay VII. In one aspect, the haloperoxidase is selected from the group consisting of: a chloride peroxidase (EC 1.11.1.10), a bromide peroxidase (EC 1.11.1.18) and an iodide peroxidase (EC 1.11.1.8). In one embodiment, the haloperoxidase is a chloroperoxidase. Suitable haloperoxidases may be isolated from different fungi, in particular from the fungus group dematiaceous hyphomycetes, such as Caldariomyces, e.g., C. fumago, Alternaria, Curvularia, e.g., C. verruculosa and C. inaequalis, Drechslera, Ulocladium and
Botrytis. Haloperoxidases may also be isolated from bacteria such as Pseudomonas, e.g., P. pyrrocinia and Streptomyces, e.g., S. aureofaciens. In one embodiment, the haloperoxidase is a vanadium haloperoxidase, i.e. a vanadate-containing haloperoxidase. In one embodiment, the haloperoxidase is derivable from Curvularia sp., in particular Curvularia verruculosa; C. verruculosa CBS 147.63 or C. verruculosa CBS 444.70 as described in WO 97/04102. In one embodiment, the haloperoxidase is vanadium haloperoxidase hpx1 from Curvularia veruculosa or a variant hereof. In one embodiment, the amino acid sequence of the haloperoxidase has at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% identity to the amino acid sequence of a haloperoxidase obtainable from Curvularia verruculosa shown in SEQ ID NO 82.
One embodiment relates to a composition comprising: a) at least 0.00001 ppm of at least one polypeptide having DNase activity (DNase), wherein the DNase is selected for the group consisting of: i) a DNase comprising one or more of the motifs [T/D/S][G/N]PQL, [F/L/Y/I]A[N/R]D[L/I/P/V], or C[D/N]T[A/R]; ii) a DNase comprising the motif [D/Q][I/V]DH; iii) a DNase comprising one or both motifs [D/M/L][S/T]GYSR[D/N] or ASXNRSKG; iv) a DNase comprising one or both motifs [V/I]PL[S/A]NAWK or NPQL; v) a DNase comprising one or both motifs P[Q/E]L[W/Y] or [K/H/E]NAW; vi) a polypeptide having DNase activity selected from: a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 1, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 2, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 3, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 4, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 5, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 6, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 7, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 8, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 9, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 10, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 11, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 12, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 13, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 14, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 15, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 16, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 17, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 18, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 19, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 20, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 21, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 22, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 23, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 24, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 25, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 26, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 27, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 28, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 29, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 30, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 31, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 32, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 33, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 34, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 35, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 36, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 37, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 38, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 39, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 40, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 41, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 42, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 43, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 44, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 45, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 46, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 47, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 48, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 49, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 50, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 51, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 52, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 53, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 54, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 55, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 56, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 57, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 58, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 59, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 60, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 61, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 62, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 63, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 64, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 65, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 66, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 67, and a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 68, and b) at least 0.00001 ppm of one haloperoxidase selected from the group consisting of: a chloride peroxidase (EC 1.11.1.10) and a bromide peroxidase (EC 1.11.1.18), preferably a vanadium haloperoxidase hpx1 from Curvularia veruculosa or a variant thereof; selected from the group consisting of: i) a polypeptide having haloperoxidase activity having at least 60%, 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99% or 100% sequence identity to the amino acid sequence shown in SEQ ID NO: 82; ii) a polypeptide having haloperoxidase activity, which is a variant of the polypeptide shown in SEQ ID NO: 82, comprising a substitution, deletion, and/or insertion at one or more positions; iii) a polypeptide having haloperoxidase activity, which is immunologically cross-reactive with the amino acid sequence set forth in SEQ ID NO: 82; and iv) a polypeptide having haloperoxidase activity, which is a fragment of the polypeptide of (a) (b) or (c); and c) at least one cleaning composition component, preferably selected from surfactants, builders, bleach components, polymers, dispersing agents and additional enzymes.
Glvcosvl hydrolase
In one embodiment, the secondary enzyme is a glycosyl hydrolase. Glycosyl hydrolases (EC 3.2.1.-), are enzymes that hydrolyze the glyosidic bond between two or more carbohydrates or between a carbohydrate and a non-carbohydrate moiety.
In one embodiment, the glycosyl hydrolases belongs to the GH39 glycosyl hydrolase family (http://www.cazv.orq/). Glycosyl hydrolases that comprises the GH39 domain may be homologues of PsIG enzymes, which are proteins that degrade the exopolysaccharide Psl. Suitable PsIG enzymes includes a PsIG from Pseudomonas aeruginosa (GenBank: AAG05625).
In one embodiment, the glycosyl hydrolase belongs to the Glyco_hydro_114 pFam domain (PF03537) family. The Glyco_hydro_114 glycosyl hydrolases may be PelA homologues. PelAand most of the family members contain a domain of unknown function, DUF297 (PF03537) and may comprise a CE4 deacetylase domain. The PelA-like glycosyl hydrolase may be obtainable from Pseudomonas protegenes Pf-5, Pseudomonas aeruginosa or Geobacter metallireducens, such as Pseudomonas protegenes Pf-5 GenBank: AAY92244,
Pseudomonas aeruginosa, GenBank: AAG06452 or Geobacter metallireducens GenBank: ABB32191.
In one embodiment, the glycosyl hydrolase belongs the GHL13 family and comprises the GHL13 domain. The GHL13 glycosyl hydrolases may in addition to the GHL13 domain comprise a CE4 domain (CE, CAZY database http://www.cazy.org/ (Coutinho & Henrissat, 1999) and have deacetylase activity. The glycosyl hydrolases may be PgaA/BpsB homologs comprising a C-terminus glycosyl hydrolase domain (GHL13) and optionally a N-terminus deacetylase domain (CE4). The glycosyl hydrolase may be obtainable from Escherichia coli K-12, or Bordetella bronchiseptica RB50, such as Bordetella bronchiseptica RB50 Genbank: CAE32265 or Escherichia coli K-12 GenBank: AAC74108.
One embodiment relates to a composition comprising: a) at least 0.00001 ppm of at least one DNase, wherein the DNase is selected from the group consisting of: i) a DNase comprising one or more of the motifs [T/D/S][G/N]PQL, [F/L/Y/I]A[N/R]D[L/I/P/V], or C[D/N]T[A/R]; ii) a DNase comprising the motif [D/Q][I/V]DH; iii) a DNase comprising one or both motifs [D/M/L][S/T]GYSR[D/N] or ASXNRSKG; iv) a DNase comprising one or both motifs [V/I]PL[S/A]NAWK or NPQL; v) a DNase comprising one or both motifs P[Q/E]L[W/Y] or [K/H/EJNAW; vi) a DNase selected from: a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 1, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 2, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 3, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 4, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 5, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 6, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 7, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 8, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 9, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 10, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 11, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 12, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 13, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 14, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 15, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 16, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 17, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 18, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 19, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 20, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 21, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 22, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 23, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 24, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 25, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 26, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 27, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 28, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 29, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 30, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 31, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 32, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 33, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 34, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 35, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 36, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 37, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 38, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 39, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 40, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 41, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 42, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 43, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 44, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 45, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 46, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 47, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 48, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 49, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 50, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 51, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 52, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 53, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 54, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 55, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 56, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 57, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 58, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 59, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 60, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 61, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 62, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 63, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 64, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 65, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 66, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 67, and a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 68, and b) at least 0.00001 ppm of one or more glycosyl hydrolase, wherein the glycosyl hydrolase is selected from the group consisting of; i) a glycosyl hydrolase from Pseudomonas aeruginosa e.g. the glycosyl hydrolase with GenBank No. AAG05625; ii) a glycosyl hydrolase, wherein the glycosyl hydrolase comprises a GH39 domain; iii) a glycosyl hydrolase from Pseudomonas protegenes Pf-5 e.g. the glycosyl hydrolase with GenBank No. AAY92244, a glycosyl hydrolase from Pseudomonas aeruginosa e.g. the glycosyl hydrolase with GenBank No. AAG06452 ora glycosyl hydrolase from Geobacter metallireducens e.g. the glycosyl hydrolase with GenBank No. ABB32191; iv) a glycosyl hydrolase comprising the Glyco_hydro_114 pFam domain (PF03537); v) a glycosyl hydrolase from Bordetella bronchiseptica RB50 e.g. the glycosyl hydrolase with Genbank number CAE32265 or a glycosyl hydrolase from Escherichia coli K-12 e.g. the glycosyl hydrolase with GenBank:AAC74108; and vi) a glycosyl hydrolase, wherein the glycosyl hydrolase comprises a GHL13 domain, and c) At least one cleaning component, preferably selected from surfactants, builders, bleach components, polymers and dispersing agents. RNase
In one embodiment, the secondary enzyme is a RNase. The term “RNase” is abbreviation of the term ribonuclease, which means a nuclease having RNase activity (EC 3.1.2X) that catalyzes the degradation of RNA into smaller components. Ribonucleases can be divided into endoribonucleases and exoribonucleases. RNase activity may be determined as shown in Assay VIII.
The RNase may be a RNases with E.C.3.1.26.X, where X = 1,2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12,13 e.g. Physarum polycephalum RNase, RNase alpha, RNase III, RNase C, RNase H, RNase Hll, RNase P, RNase IV, RNase P4, RNase M5, RNase poly-U specific, RNase IX, RNase Z, RNase E, RNase L or Retroviral RNase H.
The RNase may also be selected from EC.3.1.27. Y, where Y= 1,2, 3, 4, 5, 6, 7, 8, 9, 10 e.g. the RNases; RNase T(2), Bacillus subtilis RNase, RNase T(1), RNase U(2), Pancreatic RNase, RNase A, RNase I, Enterobacter RNase, RNase F, RNase V, rRNA endonuclease and e.g. RNase B Glycoprotein standard from bovine pancreas.
In one embodiment, the RNase is any of the following: RNase A (Bovine pancreas) UniProt -P61823, RNase HI (E. coli) Uniprot- P0A7Y4, RNase Hll (E. coli) UniProt - P10442, RNase III (E. coli) UniProt - P0A7Y0, RNase T1 (A. oryzae) UniProt - P00651 or RNase T2 (A. oryzae) Uniprot - P10281.
The RNase may also be a RNases from the PF00545 family of RNases e.g. The RNase Barnase, Swiss Prot P00648.
One embodiment relates to a composition comprising; a) at least 0.00001 ppm of at least one DNase, wherein the DNase is selected from the group consisting of: i) a DNase comprising one or more of the motifs [T/D/S][G/N]PQL, [F/L/Y/I]A[N/R]D[L/I/P/V], or C[D/N]T[A/R]; ii) a DNase comprising the motif [D/Q][I/V]DH; iii) a DNase comprising one or both motifs [D/M/L][S/T]GYSR[D/N] or ASXNRSKG; iv) a DNase comprising one or both motifs [V/I]PL[S/A]NAWK or NPQL; v) a DNase comprising one or both motifs P[Q/E]L[W/Y] or [K/H/EJNAW; vi) a DNase selected from: a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 1, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 2, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 3, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 4, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 5, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 6, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 7, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 8, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 9, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 10, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 11, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 12, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 13, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 14, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 15, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 16, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 17, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 18, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 19, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 20, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 21, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 22, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 23, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 24, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 25, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 26, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 27, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 28, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 29, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 30, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 31, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 32, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 33, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 34, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 35, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 36, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 37, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 38, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 39, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 40, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 41, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 42, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 43, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 44, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 45, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 46, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 47, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 48, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 49, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 50, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 51, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 52, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 53, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 54, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 55, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 56, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 57, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 58, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 59, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 60, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 61, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 62, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 63, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 64, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 65, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 66, a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 67, and a polypeptide having at least 80% sequence identity to the polypeptide shown in SEQ ID NO: 68, and b) at least 0.00001 ppm of one or more RNase, wherein the RNase is selected from the group consisting of; i) a RNase selected from the group of RNases comprised in E.C.3.1.26.X, where X = 1,2, 3,4, 5,6, 7, 8, 9,10,11,12,13, preferably Physarumpolycephalum RNase, RNase alpha, RNase III, RNase C, RNase H, RNase Hll, RNase P, RNase IV, RNase P4, RNase M5, RNase poly-U specific, RNase IX, RNase Z, RNase E, RNase L or Retroviral RNase H; ii) RNase selected from the group of RNases comprised in EC.3.1.27. Y, where Y= 1, 2, 3, 4, 5, 6, 7, 8, 9,10, preferably RNase T(2), Bacillus subtilis RNase, RNase T(1), RNase U(2), Pancreatic RNase, RNase A, RNase I, Enterobacter RNase, RNase F, RNase V, rRNA endonuclease or RNase B; and c) At least one cleaning component, preferably selected from surfactants, builders, bleach components, polymers and dispersing agents.
Further enzymes
The detergent additive as well as the cleaning composition may comprise one or more additional proteases, mannanases, cellulases, amylases and enzymes such as one or more lipase, cutinase, pectinase, pectate lyase, arabinase, galactanase, xylanase, oxidase, e.g., a laccase, and/or peroxidase.
In particular, the composition may comprise lipases suitable lipases and cutinases include those of bacterial or fungal origin. Chemically modified or protein engineered mutant enzymes are included. Examples include lipase from Thermomyces, e.g. from T. lanuginosus (previously named Humicola lanuginosa) as described in EP258068 and EP305216, cutinase from Humicola, e.g. H. insolens (WO96/13580), lipase from strains of Pseudomonas (some of these now renamed to Burkholderia), e.g. P. alcaligenes or P. pseudoalcaligenes (EP218272), P. cepacia (EP331376), P. sp. strain SD705 (W095/06720 & W096/27002), P. wisconsinensis (WO96/12012), GDSL-type Streptomyces lipases (W010/065455), cutinase from Magnaporthe grisea (W010/107560), cutinase from Pseudomonas mendocina (US5,389,536), lipase from Thermobifida fusca (W011/084412), Geobacillus stearothermophilus lipase (W011/084417), lipase from Bacillus subtilis (W011/084599), and lipase from Streptomyces griseus (W011/150157) and S. pristinaespiralis (W012/137147). Other examples are lipase variants such as those described in EP407225, WO92/05249, WO94/01541, W094/25578, W095/14783, WO95/30744, W095/35381, W095/22615, W096/00292, W097/04079, W097/07202, WO00/34450, WO00/60063, WO01/92502, W007/87508 and WO09/109500. Preferred commercial lipase products include Lipolase™, Lipex™; Lipolex™ and Lipoclean™ (Novozymes A/S), Lumafast (originally from Genencor) and Lipomax (originally from Gist-Brocades).
Still other examples are lipases sometimes referred to as acyltransferases or perhydrolases, e.g. acyltransferases with homology to Candida antarctica lipase A (WO10/111143), acyltransferase from Mycobacterium smegmatis (WO05/56782), perhydrolases from the CE 7 family (WO09/67279), and variants of the M. smegmatis perhydrolase in particular the S54V variant used in the commercial product Gentle Power Bleach from Huntsman Textile Effects Pte Ltd (W010/100028).
Cleaning components
The choice of cleaning components may include, for textile care, the consideration of the type of textile to be cleaned, the type and/or degree of soiling, the temperature at which cleaning is to take place, and the formulation of the detergent product. Although components mentioned below are categorized by general header according to a particular functionality, this is not to be construed as a limitation, as a component may comprise additional functionalities as will be appreciated by the skilled artisan.
Surfactants
The cleaning composition may comprise one or more surfactants, which may be anionic and/or cationic and/or non-ionic and/or semi-polar and/or zwitterionic, or a mixture thereof. In a particular embodiment, the detergent composition includes a mixture of one or more nonionic surfactants and one or more anionic surfactants. The surfactant(s) is typically present at a level of from about 0.1% to 60% by weight, such as about 1 % to about 40%, or about 3% to about 20%, or about 3% to about 10%. The surfactant(s) is chosen based on the desired cleaning application, and may include any conventional surfactant(s) known in the art.
When included therein the detergent will usually contain from about 1% to about 40% by weight of an anionic surfactant, such as from about 5% to about 30%, including from about 5% to about 15%, or from about 15% to about 20%, or from about 20% to about 25% of an anionic surfactant. Non-limiting examples of anionic surfactants include sulfates and sulfonates, in particular, linear alkylbenzenesulfonates (LAS), isomers of LAS, branched alkylbenzenesulfonates (BABS), phenylalkanesulfonates, alpha-olefinsulfonates (AOS), olefin sulfonates, alkene sulfonates, alkane-2,3-diylbis(sulfates), hydroxyalkanesulfonates and disulfonates, alkyl sulfates (AS) such as sodium dodecyl sulfate (SDS), fatty alcohol sulfates (FAS), primary alcohol sulfates (PAS), alcohol ethersulfates (AES or AEOS or FES, also known as alcohol ethoxysulfates or fatty alcohol ether sulfates), secondary alkanesulfonates (SAS), paraffin sulfonates (PS), ester sulfonates, sulfonated fatty acid glycerol esters, alpha-sulfo fatty acid methyl esters (alpha-SFMe or SES) including methyl ester sulfonate (MES), alkyl- or alkenylsuccinic acid, dodecenyl/tetradecenyl succinic acid (DTSA), fatty acid derivatives of amino acids, diesters and monoesters of sulfo-succinic acid or salt of fatty acids (soap), and combinations thereof.
When included therein the detergent will usually contain from about 1% to about 40% by weigh of a cationic surfactant, for example from about 0.5% to about 30%, in particular from about 1% to about 20%, from about 3% to about 10%, such as from about 3% to about 5%, from about 8% to about 12% or from about 10% to about 12%. Non-limiting examples of cationic surfactants include alkyldimethylethanolamine quat (ADMEAQ), cetyltrimethylammonium bromide (CTAB), dimethyldistearylammonium chloride (DSDMAC), and alkylbenzyldimethylammonium, alkyl quaternary ammonium compounds, alkoxylated quaternary ammonium (AQA) compounds, ester quats, and combinations thereof.
When included therein the detergent will usually contain from about 0.2% to about 40% by weight of a nonionic surfactant, for example from about 0.5% to about 30%, in particular from about 1% to about 20%, from about 3% to about 10%, such as from about 3% to about 5%, from about 8% to about 12%, or from about 10% to about 12%. Non-limiting examples of nonionic surfactants include alcohol ethoxylates (AE or AEO), alcohol propoxylates, propoxylated fatty alcohols (PFA), alkoxylated fatty acid alkyl esters, such as ethoxylated and/or propoxylated fatty acid alkyl esters, alkylphenol ethoxylates (APE), nonylphenol ethoxylates (NPE), alkylpolyglycosides (APG), alkoxylated amines, fatty acid monoethanolamides (FAM), fatty acid diethanolamides (FADA), ethoxylated fatty acid monoethanolamides (EFAM), propoxylated fatty acid monoethanolamides (PFAM), polyhydroxyalkyl fatty acid amides, or N-acyl N-alkyl derivatives of glucosamine (glucamides, GA, or fatty acid glucamides, FAGA), as well as products available under the trade names SPAN and TWEEN, and combinations thereof.
When included therein the detergent will usually contain from about 0.01 to about 10 % by weight of a semipolar surfactant. Non-limiting examples of semipolar surfactants include amine oxides (AO) such as alkyldimethylamineoxide, N-(coco alkyl)-N,N-dimethylamine oxide and N-(tallow-alkyl)-N,N-bis(2-hydroxyethyl)amine oxide and combinations thereof.
When included therein the detergent will usually contain from about 0.01 % to about 10 % by weight of a zwitterionic surfactant. Non-limiting examples of zwitterionic surfactants include betaines such as alkyldimethylbetaines, sulfobetaines, and combinations thereof.
Builders and Co-Builders
The cleaning composition may contain about 0-65% by weight, such as about 5% to about 50% of a detergent builder or co-builder, or a mixture thereof. In a dish wash detergent, the level of builder is typically 40-65%, particularly 50-65%. The builder and/or co-builder may particularly be a chelating agent that forms water-soluble complexes with Ca and Mg. Any builder and/or co-builder known in the art for use in cleaning detergents may be utilized. Non-limiting examples of builders include zeolites, diphosphates (pyrophosphates), triphosphates such as sodium triphosphate (STP or STPP), carbonates such as sodium carbonate, soluble silicates such as sodium metasilicate, layered silicates (e.g., SKS-6 from Hoechst), ethanolamines such as 2-aminoethan-1-ol (MEA), diethanolamine (DEA, also known as 2,2’-iminodiethan-1-ol), triethanolamine (TEA, also known as 2,2’,2”-nitrilotriethan-1-ol), and (carboxymethyl)inulin (CMI), and combinations thereof.
The cleaning composition may also contain 0-50% by weight, such as about 5% to about 30%, of a detergent co-builder. The detergent composition may include a co-builder alone, or in combination with a builder, for example a zeolite builder. Non-limiting examples of co-builders include homopolymers of polyacrylates or copolymers thereof, such as poly(acrylic acid) (PAA) or copoly(acrylic acid/maleic acid) (PAA/PMA). Further non-limiting examples include citrate, chelators such as aminocarboxylates, aminopolycarboxylates and phosphonates, and alkyl- or alkenylsuccinic acid. Additional specific examples include 2,2’,2”-nitrilotriacetic acid (NTA), ethylenediaminetetraacetic acid (EDTA), diethylenetriaminepentaacetic acid (DTPA), iminodisuccinic acid (IDS), ethylenediamine-N,N’-disuccinic acid (EDDS), methylglycinediacetic acid (MGDA), glutamic acid-N,N-diacetic acid (GLDA), 1-hydroxyethane-1,1-diphosphonic acid (HEDP), ethylenediaminetetra(methylenephosphonic acid) (EDTMPA), diethylenetriaminepentakis(methylenephosphonic acid) (DTMPA or DTPMPA), N-(2-hydroxyethyl)iminodiacetic acid (EDG), aspartic acid-N-monoacetic acid (ASMA), aspartic acid-N,N-diaceticacid (ASDA), aspartic acid-N-monopropionic acid (ASMP), iminodisuccinic acid (IDA), N-(2-sulfomethyl)-aspartic acid (SMAS), N-(2-sulfoethyl)-aspartic acid (SEAS), N-(2-sulfomethyl)-glutamic acid (SMGL), N-(2-sulfoethyl)-glutamic acid (SEGL), N-methyliminodiacetic acid (MlDA), a-alanine-N,N-diacetic acid (α-ALDA), serine-N,N-diacetic acid (SEDA), isoserine-N,N-diacetic acid (ISDA), phenylalanine-N,N-diacetic acid (PHDA), anthranilic acid-N,N-diacetic acid (ANDA), sulfanilic acid-N,N-diacetic acid (SLDA) , taurine-N,N-diacetic acid (TUDA) and sulfomethyl-N,N-diacetic acid (SMDA), N-(2-hydroxyethyl)ethylenediamine-N,N’,N”-triacetic acid (HEDTA), diethanolglycine (DEG), diethylenetriamine penta(methylenephosphonic acid) (DTPMP), aminotris(methylenephosphonic acid) (ATMP), and combinations and salts thereof. Further exemplary builders and/or co-builders are described in, e.g., WO 09/102854, US 5977053
Bleaching Systems
The cleaning composition may contain 0-30% by weight, such as about 1% to about 20%, of a bleaching system. Any bleaching system comprising components known in the art for use in cleaning detergents may be utilized. Suitable bleaching system components include sources of hydrogen peroxide; sources of peracids; and bleach catalysts or boosters.
Sources of hydrogen peroxide:
Suitable sources of hydrogen peroxide are inorganic persalts, including alkali metal salts such as sodium percarbonate and sodium perborates (usually mono- or tetrahydrate), and hydrogen peroxide—urea (1/1).
Sources of peracids:
Peracids may be (a) incorporated directly as preformed peracids or (b) formed in situ in the wash liquor from hydrogen peroxide and a bleach activator (perhydrolysis) or (c) formed in situ in the wash liquor from hydrogen peroxide and a perhydrolase and a suitable substrate for the latter, e.g., an ester. a) Suitable preformed peracids include, but are not limited to, peroxycarboxylic acids such as peroxybenzoic acid and its ring-substituted derivatives, peroxy-a-naphthoic acid, peroxyphthalic acid, peroxylauric acid, peroxystearic acid, ε-phthalimidoperoxycaproic acid [phthalimidoperoxyhexanoic acid (PAP)], and o-carboxybenzamidoperoxycaproic acid; aliphatic and aromatic diperoxydicarboxylic acids such as diperoxydodecanedioic acid, diperoxyazelaic acid, diperoxysebacic acid, diperoxybrassylic acid, 2-decyldiperoxybutanedioic acid, and diperoxyphthalic, -isophthalic and -terephthalic acids; perimidic acids; peroxymonosulfuric acid; peroxydisulfuric acid; peroxyphosphoric acid; peroxysilicic acid; and mixtures of said compounds. It is understood that the peracids mentioned may in some cases be best added as suitable salts, such as alkali metal salts (e.g., Oxone®) or alkaline earth-metal salts. b) Suitable bleach activators include those belonging to the class of esters, amides, imides, nitriles or anhydrides and, where applicable, salts thereof. Suitable examples are tetraacetylethylenediamine (TAED), sodium 4-[(3,5,5-trimethylhexanoyl)oxy]benzene-1-sulfonate (ISONOBS), sodium 4-(dodecanoyloxy)benzene-1-sulfonate (LOBS), sodium 4-(decanoyloxy)benzene-l-sulfonate, 4-(decanoyloxy)benzoic acid (DOBA), sodium 4-(nonanoyloxy)benzene-l-sulfonate (NOBS), and/or those disclosed in W098/17767. A particular family of bleach activators of interest was disclosed in EP624154 and particularly preferred in that family is acetyl triethyl citrate (ATC). ATC or a short chain triglyceride like triacetin has the advantage that they are environmentally friendly. Furthermore, acetyl triethyl citrate and triacetin have good hydrolytical stability in the product upon storage and are efficient bleach activators. Finally, ATC is multifunctional, as the citrate released in the perhydrolysis reaction may function as a builder.
Bleach catalysts and boosters
The bleaching system may also include a bleach catalyst or booster.
Some non-limiting examples of bleach catalysts that may be used in the compositions include manganese oxalate, manganese acetate, manganese-collagen, cobalt-amine catalysts and manganese triazacyclononane (MnTACN) catalysts; particularly preferred are complexes of manganese with 1,4,7-trimethyl-1,4,7-triazacyclononane (Me3-TACN) or 1,2,4,7-tetramethyl-1,4,7-triazacyclononane (Me4-TACN), in particular Me3-TACN, such as the dinuclear manganese complex [(Me3-TACN)Mn(0)3Mn(Me3-TACN)](PF6)2, and [2,2',2"-nitrilotris(ethane-1,2-diylazanylylidene-KN-methanylylidene)triphenolato-K30]manganese(lll). The bleach catalysts may also be other metal compounds; such as iron or cobalt complexes.
In some embodiments, where a source of a peracid is included, an organic bleach catalyst or bleach booster may be used having one of the following formulae:
(iii) and mixtures thereof; wherein each R1 is independently a branched alkyl group containing from 9 to 24 carbons or linear alkyl group containing from 11 to 24 carbons, preferably each R1 is independently a branched alkyl group containing from 9 to 18 carbons or linear alkyl group containing from 11 to 18 carbons, more preferably each R1 is independently selected from the group consisting of 2-propylheptyl, 2-butyloctyl, 2-pentylnonyl, 2-hexyldecyl, dodecyl, tetradecyl, hexadecyl, octadecyl, isononyl, isodecyl, isotridecyl and isopentadecyl.
Other exemplary bleaching systems are described, e.g. in W02007/087258, W02007/087244, W02007/087259, EP1867708 (Vitamin K) and W02007/087242. Suitable photobleaches may for example be sulfonated zinc or aluminium phthalocyanines.
Metal care agents
Metal care agents may prevent or reduce the tarnishing, corrosion or oxidation of metals, including aluminium, stainless steel and non-ferrous metals, such as silver and copper. Suitable examples include one or more of the following: (a) benzatriazoles, including benzotriazole or bis-benzotriazole and substituted derivatives thereof. Benzotriazole derivatives are those compounds in which the available substitution sites on the aromatic ring are partially or completely substituted. Suitable substituents include linear or branch-chain Ci-C20- alkyl groups (e.g., C1-C20- alkyl groups) and hydroxyl, thio, phenyl or halogen such as fluorine, chlorine, bromine and iodine. (b) metal salts and complexes chosen from the group consisting of zinc, manganese, titanium, zirconium, hafnium, vanadium, cobalt, gallium and cerium salts and/or complexes, the metals being in one of the oxidation states II, III, IV, V or VI. In one aspect, suitable metal salts and/or metal complexes may be chosen from the group consisting of Mn(ll) sulphate, Mn(ll) citrate, Mn(ll) stearate, Mn(ll) acetylacetonate, KATiF6 (e.g., K2TiF6), KAZrF6 (e.g., K2ZrF6), CoS04, Co(NOs)2 and Ce(NOs)3, zinc salts, for example zinc sulphate, hydrozincite or zinc acetate.; (c) silicates, including sodium or potassium silicate, sodium disilicate, sodium metasilicate, crystalline phyllosilicate and mixtures thereof.
Further suitable organic and inorganic redox-active substances that act as silver/copper corrosion inhibitors are disclosed in WO 94/26860 and WO 94/26859. Preferablv the comDOsition comprises from 0.1 to 5% by weight of the composition of a metal care agent, preferably the metal care agent is a zinc salt.
Hydrotropes
The cleaning composition may contain 0-10% by weight, for example 0-5% by weight, such as about 0.5 to about 5%, or about 3% to about 5%, of a hydrotrope. Any hydrotrope known in the art for use in detergents may be utilized. Non-limiting examples of hydrotropes include sodium benzenesulfonate, sodium p-toluene sulfonate (STS), sodium xylene sulfonate (SXS), sodium cumene sulfonate (SCS), sodium cymene sulfonate, amine oxides, alcohols and polyglycolethers, sodium hydroxynaphthoate, sodium hydroxynaphthalene sulfonate, sodium ethylhexyl sulfate, and combinations thereof.
Polymers
The cleaning composition may contain 0-10% by weight, such as 0.5-5%, 2-5%, 0.5-2% or 0.2-1 % of a polymer. Any polymer known in the art for use in detergents may be utilized. The polymer may function as a co-builder as mentioned above, or may provide antiredeposition, fiber protection, soil release, dye transfer inhibition, grease cleaning and/or anti-foaming properties. Some polymers may have more than one of the above-mentioned properties and/or more than one of the below-mentioned motifs. Exemplary polymers include (carboxymethyl)cellulose (CMC), poly(vinyl alcohol) (PVA), poly(vinylpyrrolidone) (PVP), poly(ethyleneglycol) or polyethylene oxide) (PEG), ethoxylated poly(ethyleneimine), carboxymethyl inulin (CMI), and polycarboxylates such as PAA, PAA/PMA, poly-aspartic acid, and lauryl methacrylate/acrylic acid copolymers , hydrophobically modified CMC (HM-CMC) and silicones, copolymers of terephthalic acid and oligomeric glycols, copolymers of polyethylene terephthalate) and poly(oxyethene terephthalate) (PET-POET), PVP, poly(vinylimidazole) (PVI), poly(vinylpyridine-N-oxide) (PVPO or PVPNO) and polyvinylpyrrolidone-vinylimidazole (PVPVI). Suitable examples include PVP-K15, PVP-K30, ChromaBond S-400, ChromaBond S- 403E and Chromabond S-100 from Ashland Aqualon, and Sokalan® HP 165, Sokalan® HP 50 (Dispersing agent), Sokalan® HP 53 (Dispersing agent), Sokalan® HP 59 (Dispersing agent), Sokalan® HP 56 (dye transfer inhibitor), Sokalan® HP 66 K (dye transfer inhibitor) from BASF. Further exemplary polymers include sulfonated polycarboxylates, polyethylene oxide and polypropylene oxide (PEO-PPO) and diquaternium ethoxy sulfate. Other exemplary polymers are disclosed in, e.g., WO 2006/130575. Salts of the above-mentioned polymers are also contemplated. Particularly preferred polymer is ethoxylated homopolymer Sokalan® HP 20 from BASF, which helps to prevent redeposition of soil in the wash liquor.
Dispersants
The cleaning composition may also comprise dispersants. In particular, powdered detergents may comprise dispersants. Suitable water-soluble organic materials include the homo- or co polymeric acids or their salts, in which the polycarboxylic acid comprises at least two carboxyl radicals separated from each other by not more than two carbon atoms. Suitable dispersants are for example described in Powdered Detergents, Surfactant science series volume 71, Marcel Dekker, Inc.
Dye Transfer Inhibiting Agents
The cleaning composition may also include one or more dye transfer inhibiting agents. Suitable polymeric dye transfer inhibiting agents include, but are not limited to, polyvinylpyrrolidone polymers, polyamine N-oxide polymers, copolymers of N-vinylpyrrolidone and N-vinylimidazole, polyvinyloxazolidones and polyvinylimidazoles or mixtures thereof. When present in a subject composition, the dye transfer inhibiting agents may be present at levels from about 0.0001 % to about 10%, from about 0.01% to about 5% or even from about 0.1% to about 3% by weight of the composition.
Fluorescent whitening agent
The cleaning composition may also comprise additional components that may tint articles being cleaned, such as fluorescent whitening agent or optical brighteners. Where present the brightener is preferably at a level of about 0.01% to about 0.5%. Any fluorescent whitening agent suitable for use in a laundry detergent composition may be used in the composition. The most commonly used fluorescent whitening agents are those belonging to the classes of diaminostilbene-sulfonic acid derivatives, diarylpyrazoline derivatives and bisphenyl-distyryl derivatives. Examples of the diaminostilbene-sulfonic acid derivative type of fluorescent whitening agents include the sodium salts of: 4,4'-bis-(2-diethanolamino-4-anilino-s-triazin-6-ylamino) stilbene-2,2'-disulfonate, 4,4'-bis-(2,4-dianilino-s-triazin-6-ylamino) stilbene-2.2'-disulfonate, 4,4'-bis-(2-anilino-4-(N-methyl-N-2-hydroxy-ethylamino)-s-triazin-6-ylamino) stilbene-2,2'-disulfonate, 4,4'-bis-(4-phenyl-1,2,3-triazol-2-yl)stilbene-2,2'-disulfonate and sodium 5-(2H-naphtho[1,2-d][1,2,3]triazol-2-yl)-2-[(E)-2-phenylvinyljbenzenesulfonate. Preferred fluorescent whitening agents are Tinopal DMS and Tinopal CBS available from Ciba-Geigy AG, Basel, Switzerland. Tinopal DMS is the disodium salt of 4,4'-bis-(2-morpholino-4-anilino-s-triazin-6-ylamino) stilbene-2,2'-disulfonate. Tinopal CBS is the disodium salt of 2,2'-bis-(phenyl-styryl)-disulfonate. Also preferred are fluorescent whitening agents is the commercially available Parawhite KX, supplied by Paramount Minerals and Chemicals, Mumbai, India. Other fluorescers suitable for use include the 1-3-diaryl pyrazolines and the 7-alkylaminocoumarins. Suitable fluorescent brightener levels include lower levels of from about 0.01, from 0.05, from about 0.1 or even from about 0.2 wt % to upper levels of 0.5 or even 0.75 wt%.
Soil release polymers
The cleaning composition may also include one or more soil release polymers which aid the removal of soils from fabrics such as cotton and polyester based fabrics, in particular the removal of hydrophobic soils from polyester based fabrics. The soil release polymers may for example be nonionic or anionic terephthalte based polymers, polyvinyl caprolactam and related copolymers, vinyl graft copolymers, polyester polyamides see for example Chapter 7 in Powdered Detergents, Surfactant science series volume 71, Marcel Dekker, Inc. Another type of soil release polymers is amphiphilic alkoxylated grease cleaning polymers comprising a core structure and a plurality of alkoxylate groups attached to that core structure. The core structure may comprise a polyalkylenimine structure or a polyalkanolamine structure as described in detail in WO 2009/087523 (hereby incorporated by reference). Furthermore, random graft co-polymers are suitable soil release polymers. Suitable graft co-polymers are described in more detail in WO 2007/138054, WO 2006/108856 and WO 2006/113314 (hereby incorporated by reference). Suitable polyethylene glycol polymers include random graft co-polymers comprising: (i) hydrophilic backbone comprising polyethylene glycol; and (ii) side chain(s) selected from the group consisting of: C4-C25 alkyl group, polypropylene, polybutylene, vinyl ester of a saturated C1-C6 mono-carboxylic acid, Cl-C 6 alkyl ester of acrylic or methacrylic acid, and mixtures thereof. Suitable polyethylene glycol polymers have a polyethylene glycol backbone with random grafted polyvinyl acetate side chains. The average molecular weight of the polyethylene glycol backbone can be in the range of from 2,000 Da to 20,000 Da, or from 4,000 Da to 8,000 Da. The molecular weight ratio of the polyethylene glycol backbone to the polyvinyl acetate side chains can be in the range of from 1: 1 to 1:5, or from 1: 1.2 to 1:2. The average number of graft sites per ethylene oxide units can be less than 1, or less than 0.8, the average number of graft sites per ethylene oxide units can be in the range of from 0.5 to 0.9, or the average number of graft sites per ethylene oxide units can be in the range of from 0.1 to 0.5, or from 0.2 to 0.4. A suitable polyethylene glycol polymer is Sokalan HP22. Other soil release polymers are substituted polysaccharide structures especially substituted cellulosic structures such as modified cellulose deriviatives such as those described in EP 1867808 or WO 2003/040279 (both are hereby incorporated by reference). Suitable cellulosic polymers include cellulose, cellulose ethers, cellulose esters, cellulose amides and mixtures thereof. Suitable cellulosic polymers include anionically modified cellulose, nonionically modified cellulose, cationically modified cellulose, zwitterionically modified cellulose, and mixtures thereof. Suitable cellulosic polymers include methyl cellulose, carboxy methyl cellulose, ethyl cellulose, hydroxyl ethyl cellulose, hydroxyl propyl methyl cellulose, ester carboxy methyl cellulose, and mixtures thereof.
Anti-redeposition agents
The cleaning composition may also include one or more anti-redeposition agents such as carboxymethylcellulose (CMC), polyvinyl alcohol (PVA), polyvinylpyrrolidone (PVP), polyoxyethylene and/or polyethyleneglycol (PEG), homopolymers of acrylic acid, copolymers of acrylic acid and maleic acid, and ethoxylated polyethyleneimines. The cellulose based polymers described under soil release polymers above may also function as anti-redeposition agents.
Rheology Modifiers
The cleaning composition may also include one or more rheology modifiers, structurants or thickeners, as distinct from viscosity reducing agents. The rheology modifiers are selected from the group consisting of non-polymeric crystalline, hydroxy-functional materials, polymeric rheology modifiers which impart shear thinning characteristics to the aqueous liquid matrix of a liquid detergent composition. The rheology and viscosity of the detergent can be modified and adjusted by methods known in the art, for example as shown in EP 2169040.
Other suitable cleaning composition components include, but are not limited to, antishrink agents, anti-wrinkling agents, bactericides, binders, carriers, dyes, enzyme stabilizers, fillers, foam regulators, hydrotropes, perfumes, pigments, sod suppressors, solvents, and structurants for liquid detergents and/or structure elasticizing agents.
Formulation of cleaning products
The cleaning composition may be in any convenient form, e.g., a bar, a homogenous tablet, a tablet having two or more layers, a pouch having one or more compartments, a regular or compact powder, a granule, a paste, a gel, or a regular, compact or concentrated liquid. Pouches can be configured as single or multicompartments. It can be of any form, shape and material which is suitable for hold the composition, e.g. without allowing the release of the composition to release of the composition from the pouch prior to water contact. The pouch is made from water soluble film which encloses an inner volume. Said inner volume can be divided into compartments of the pouch. Preferred films are polymeric materials preferably polymers which are formed into a film or sheet. Preferred polymers, copolymers or derivates thereof are selected polyacrylates, and water soluble acrylate copolymers, methyl cellulose, carboxy methyl cellulose, sodium dextrin, ethyl cellulose, hydroxyethyl cellulose, hydroxypropyl methyl cellulose, malto dextrin, poly methacrylates, most preferably polyvinyl alcohol copolymers and, hydroxypropyl methyl cellulose (HPMC). Preferably the level of polymer in the film for example PVA is at least about 60%. Preferred average molecular weight will typically be about 20,000 to about 150,000. Films can also be of blended compositions comprising hydrolytically degradable and water soluble polymer blends such as polylactide and polyvinyl alcohol (known under the Trade reference M8630 as sold by MonoSol LLC, Indiana, USA) plus plasticisers like glycerol, ethylene glycerol, propylene glycol, sorbitol and mixtures thereof. The pouches can comprise a solid laundry cleaning composition or part components and/or a liquid cleaning composition or part components separated by the water soluble film. The compartment for liquid components can be different in composition than compartments containing solids: US2009/0011970 A1. Detergent ingredients can be separated physically from each other by compartments in water dissolvable pouches or in different layers of tablets. Thereby negative storage interaction between components can be avoided. Different dissolution profiles of each of the compartments can also give rise to delayed dissolution of selected components in the wash solution. A liquid or gel detergent, which is not unit dosed, may be aqueous, typically containing at least 20% by weight and up to 95% water, such as up to about 70% water, up to about 65% water, up to about 55% water, up to about 45% water, up to about 35% water. Other types of liquids, including without limitation, alkanols, amines, diols, ethers and polyols may be included in an aqueous liquid or gel. An aqueous liquid or gel detergent may contain from 0-30% organic solvent. A liquid or gel detergent may be non-aqueous.
Granular detergent formulations
The composition may be formulated as a granule for example as a co-granule that combines one or more enzymes. Each enzyme will then be present in more granules securing a more uniform distribution of enzymes in the detergent. This also reduces the physical segregation of different enzymes due to different particle sizes. Methods for producing multi-enzyme co-granulates for the detergent industry are disclosed in the IP.com disclosure IPCOM000200739D.
Another example of formulation of enzymes using co-granulates are disclosed in WO 2013/188331, which relates to a detergent composition comprising (a) a multi-enzyme co- granule; (b) less than 10 wt zeolite (anhydrous basis); and (c) less than 10 wt phosphate salt (anhydrous basis), and the composition additionally comprises from 20 to 80 wt% detergent moisture sink components. The multi-enzyme co-granule may comprise one or more enzymes selected from the group consisting of protease, amylase, lipases, cellulases, xyloglucanases, perhydrolases, peroxidases, lipoxygenases, laccases, hemicellulases, proteases, cellobiose dehydrogenases, xylanases, phospholipases, esterases, cutinases, pectinases, mannanases, pectate lyases, keratinases, reductases, oxidases, phenoloxidases, ligninases, pullulanases, tannases, pentosanases, lichenases glucanases, arabinosidases, hyaluronidase, chondroitinase and mixtures thereof.
Use of cleaning composition
On embodiment, relates to methods for using the compositions thereof. Laundry/textile/fabric (House hold laundry washing, Industrial laundry washing). Hard surface cleaning (ADW, car wash, Industrial surface)
The cleaning composition may be formulated, for example, as a hand or machine laundry detergent composition including a laundry additive composition suitable for pre-treatment of stained fabrics. In a specific aspect, relates to a detergent additive comprising one or more enzymes as described herein.
Definitions
Detergent adjunct ingredient: The detergent adjunct ingredient is different to the enzymes. The precise nature of these additional adjunct components, and levels of incorporation thereof, will depend on the physical form of the composition and the nature of the operation for which it is to be used. Suitable adjunct materials include, but are not limited to the components described below such as surfactants, builders, flocculating aid, chelating agents, dye transfer inhibitors, enzymes, enzyme stabilizers, enzyme inhibitors, catalytic materials, bleach activators, hydrogen peroxide, sources of hydrogen peroxide, preformed peracids, polymeric agents, clay soil removal/anti-redeposition agents, brighteners, suds suppressors, dyes, perfumes, structure elasticizing agents, carriers, hydrotropes, builders and co-builders, fabric anti-foaming agents, dispersants, processing aids, and/or pigments.
Cleaning composition: The term “cleaning composition” refers to compositions that find use in the removal of undesired compounds from items to be cleaned, such as textiles. The detergent composition may be used to e.g. clean textiles for both household cleaning and industrial cleaning. The terms encompass any materials/compounds selected for the particular type of cleaning composition desired and the form of the product (e.g., liquid, gel, powder, granulate, paste, or spray compositions) and includes, but is not limited to, detergent compositions (e.g., liquid and/or solid laundry detergents, fine fabric detergents and textile and laundry pre-spotters/pretreatment). In addition to containing the enzymes, the detergent formulation may contain one or more additional enzymes (such as amylases, lipases, cutinases, cellulases, endoglucanases, xyloglucanases, pectinases, pectin lyases, xanthanases, peroxidases, haloperoxygenases, catalases and mannanases, or any mixture thereof), and/or detergent adjunct ingredients such as surfactants, builders, chelators or chelating agents, bleach system or bleach components, polymers, fabric conditioners, foam boosters, suds suppressors, dyes, perfume, tannish inhibitors, optical brighteners, bactericides, fungicides, soil suspending agents, anti-corrosion agents, enzyme inhibitors or stabilizers, enzyme activators, transferase(s), hydrolytic enzymes, oxido reductases, bluing agents and fluorescent dyes, antioxidants, and solubilizers.
The term “enzyme detergency benefit” is defined herein as the advantageous effect an enzyme may add to a detergent compared to the same detergent without the enzyme. Important detergency benefits which can be provided by enzymes are stain removal with no or very little visible soils after washing and/or cleaning, prevention or reduction of redeposition of soils released in the washing process (an effect that also is termed anti-redeposition), restoring fully or partly the whiteness of textiles which originally were white but after repeated use and wash have obtained a greyish or yellowish appearance (an effect that also is termed whitening). Textile care benefits, which are not directly related to catalytic stain removal or prevention of redeposition of soils, are also important for enzyme detergency benefits. Examples of such textile care benefits are prevention or reduction of dye transfer from one fabric to another fabric or another part of the same fabric (an effect that is also termed dye transfer inhibition or anti-backstaining), removal of protruding or broken fibers from a fabric surface to decrease pilling tendencies or remove already existing pills or fuzz (an effect that also is termed anti-pilling), colour clarification of the fabric and removal of particulate soils which are trapped in the fibers of the fabric or garment. Enzymatic bleaching is a further enzyme detergency benefit where the catalytic activity generally is used to catalyze the formation of bleaching components such as hydrogen peroxide or other peroxides. Textile care benefits, which are not directly related to catalytic stain removal or prevention of redeposition of soils, are also important for enzyme detergency benefits. Examples of such textile care benefits are prevention or reduction of dye transfer from one textile to another textile or another part of the same textile (an effect that is also termed dye transfer inhibition or anti-backstaining), removal of protruding or broken fibers from a textile surface to decrease pilling tendencies or remove already existing pills or fuzz (an effect that also is termed anti-pilling), improvement of the textile-softness, colour clarification of the textile and removal of particulate soils which are trapped in the fibers of the textile. Enzymatic bleaching is a further enzyme detergency benefit where the catalytic activity generally is used to catalyze the formation of bleaching component such as hydrogen peroxide or other peroxides or other bleaching species.” The term “hard surface cleaning” is defined herein as cleaning of hard surfaces wherein hard surfaces may include floors, tables, walls, roofs etc. as well as surfaces of hard objects such as cars (carwash) and dishes (dish wash). Dish washing includes but are not limited to cleaning of plates, cups, glasses, bowls, cutlery such as spoons, knives, forks, serving utensils, ceramics, plastics, metals, china, glass and acrylics.
The term “wash performance” is used as an enzyme’s ability to remove stains present on the object to be cleaned during e.g. wash or hard surface cleaning.
The term “whiteness” is defined herein as a greying, yellowing of a textile. Loss of whiteness may be due to removal of optical brighteners/hueing agents. Greying and yellowing can be due to soil redeposition, body soils, colouring from e.g. iron and copper ions or dye transfer. Whiteness might include one or several issues from the list below: colourant or dye effects; incomplete stain removal (e.g. body soils, sebum etc.); redeposition (greying, yellowing or other discolourations of the object) (removed soils reassociate with other parts of textile, soiled or unsoiled); chemical changes in textile during application; and clarification or brightening of colours.
The term “laundering” relates to both household laundering and industrial laundering and means the process of treating textiles with a solution containing a cleaning or detergent composition. The laundering process can for example be carried out using e.g. a household or an industrial washing machine or can be carried out by hand.
By the term ’’malodor” is meant an odor which is not desired on clean items. The cleaned item should smell fresh and clean without malodors adhered to the item. One example of malodor is compounds with an unpleasant smell, which may be produced by microorganisms. Another example is unpleasant smells can be sweat or body odor adhered to an item which has been in contact with human or animal. Another example of malodor can be the odor from spices, which sticks to items for example curry or other exotic spices which smells strongly.
The term “mature polypeptide” means a polypeptide in its final form following translation and any post-translational modifications, such as N-terminal processing, C-terminal truncation, glycosylation, phosphorylation, etc.
The term “textile” means any textile material including yarns, yarn intermediates, fibers, non-woven materials, natural materials, synthetic materials, and any other textile material, fabrics made of these materials and products made from fabrics (e.g., garments and other articles). The textile or fabric may be in the form of knits, wovens, denims, non-wovens, felts, yarns, and towelling. The textile may be cellulose based such as natural cellulosics, including cotton, flax/linen, jute, ramie, sisal or coir or manmade cellulosics (e.g. originating from wood pulp) including viscose/rayon, cellulose acetate fibers (tricell), lyocell or blends thereof. The textile or fabric may also be non-cellulose based such as natural polyamides including wool, camel, cashmere, mohair, rabbit and silk or synthetic polymers such as nylon, aramid, polyester, acrylic, polypropylene and spandex/elastane, or blends thereof as well as blends of cellulose based and non-cellulose based fibers. Examples of blends are blends of cotton and/or rayon/viscose with one or more companion material such as wool, synthetic fiber (e.g. polyamide fiber, acrylic fiber, polyester fiber, polyvinyl chloride fiber, polyurethane fiber, polyurea fiber, aramid fiber), and/or cellulose-containing fiber (e.g. rayon/viscose, ramie, flax/linen, jute, cellulose acetate fiber, lyocell). Fabric may be conventional washable laundry, for example stained household laundry. When the term fabric or garment is used, it is intended to include the broader term textiles as well.
The term “variant” means a polypeptide having the activity of the parent or precursor polypeptide and comprising an alteration, i.e., a substitution, insertion, and/or deletion, at one or more (e.g., several) positions compared to the precursor or parent polypeptide. A substitution means replacement of the amino acid occupying a position with a different amino acid; a deletion means removal of the amino acid occupying a position; and an insertion means adding an amino acid adjacent to and immediately following the amino acid occupying a position.
Sequence identity: The relatedness between two amino acid sequences or between two nucleotide sequences is described by the parameter “sequence identity”. The sequence identity between two amino acid sequences may be determined using the Needleman-Wunsch algorithm (Needleman and Wunsch, 1970, J. Mol. Biol. 48:443-453) as implemented in the Needle program of the EMBOSS package (EMBOSS: The European Molecular Biology Open Software Suite, Rice et al., 2000, Trends Genet. 16: 276-277), preferably version 6.6.0 or later. The parameters used are a gap open penalty of 10, a gap extension penalty of 0.5, and the EBLOSUM62 (EMBOSS version of BLOSUM62) substitution matrix. The output of Needle labeled “longest identity” (obtained using the -nobrief option) is used as the percent identity and is calculated as follows: (Identical Residues x 100)/(Length of Alignment-Total Number of Gaps in Alignment)
Examples
Assays
Assay I: testing of DNase activity DNase activity may be determined on DNase Test Agar with Methyl Green (BD, Franklin Lakes, NJ, USA), which may be prepared according to the manual from supplier. Briefly, 21 g of agar was dissolved in 500 ml water and then autoclaved for 15 min at 121°C. Autoclaved agar was temperated to 48°C in water bath, and 20 ml of agar was poured into petridishes with and allowed to solidify by incubation o/n at room temperature. On solidified agar plates, 5 pi of enzyme solutions are added and DNase activity is observed as colorless zones around the spotted enzyme solutions.
Assay II: testing of Protease activity
Proteolytic activity can be determined by a method employing Suc-AAPF-PNA as the substrate. Suc-AAPF-PNA is an abbreviation for N-Succinyl-Alanine-Alanine-Proline-Phenylalanine-p-Nitroanilide, and is a blocked peptide which can be cleaved by endo-proteases. Following cleavage a free PNA molecule is liberated, which has a yellow color and thus can be measured by visible spectrophotometry at wavelength 405 nm. The Suc-AAPF-PNA substrate is manufactured by Bachem (cat. no. L1400, dissolved in DMSO). The protease sample to be analyzed is diluted in residual activity buffer (100 mM Tris pH 8.6). The assay is performed by transferring 3 0 μΙ of diluted enzyme samples to 96 well microtiter plate and adding 70 μΙ substrate working solution (0.72 mg/ml in 100 mM Tris pH8.6). The solution was mixed at room temperature and absorption is measured every 20 seconds over 5 minutes at OD 405 nm.
The slope (absorbance per minute) of the time dependent absorption-curve is directly proportional to the activity of the protease in question under the given set of conditions. The protease sample is diluted to a level where the slope is linear.
Assay III: testing of mannanase activity
Mannanase activity may be tested according to standard test procedures known in the art, such as by applying a solution to be tested to 4 mm diameter holes punched out in agar plates containing 0.2% AZCL galactomannan (carob), i.e. substrate for the assay of endo-1,4-beta-D-mannanase available as CatNo.l-AZGMA from the company Megazyme (Megazyme’s Internet address: http://www.megazyme.com/Purchase/index.html).
Assay IV: testing of xyloglucanase activity
The reaction involves endo hydrolysis of 1,4-beta-D-glucosidic linkages in xyloglucan. Xyloglucanase activity may be determined using AZCL-xyloglucan (from Megazyme) as the reaction substrate. The assay can be performed in several ways, e.g. as described in Example 2 of the present application or as described in WO 01/62903. One unit of xyloglucanase activity (XyloU) is defined by reference to the assay method described in WO 01/62903, page 60, lines 3-17.
Assay V: testing of cellulase activity
The term “cellulase activity” is defined herein as an enzyme catalyzed hydrolysis of 1,4-beta-D-glucosidic linkages in beta-1,4-glucan (cellulose). Cellulase activity may be determined using AZCL- HE-cellulose (from Megazyme) as the reaction substrate.
Assay VI: testing Amylase activity
Alpha-amylase activity may be determined by a method employing Phadebas® tablets as substrate. Phadebas tablets (Phadebas® Amylase Test, supplied by Pharmacia Diagnostic) contain a cross-linked insoluble blue-colored starch polymer, which has been mixed with bovine serum albumin and a buffer substance and tableted. For every single measurement one tablet is suspended in a tube containing 5 ml 100 mM Britton-Robinson buffer (100 mM acetic acid, 100 mM phosphoric acid, 100 mM boric acid, 0.1 mM CaCI2, pH adjusted to the value of interest with NaOH). The test is performed in a water bath at the temperature of interest. The alpha-amylase to be tested is diluted in x ml of 100 mM Britton-Robinson buffer. 1 ml of this alpha-amylase solution is added to the 5 ml 100 mM Britton-Robinson buffer. The starch is hydrolyzed by the alpha-amylase giving soluble blue fragments. The absorbance of the resulting blue solution, measured spectrophotometrically at 620 nm, is a function of the alpha-amylase activity. It is important that the measured 620 nm absorbance after 10 or 15 minutes of incubation (testing time) is in the range of 0.2 to 2.0 absorbance units at 620 nm. In this absorbance range there is linearity between activity and absorbance (Lambert-Beer law). The dilution of the enzyme must therefore be adjusted to fit this criterion. Under a specified set of conditions (temp., pH, reaction time, buffer conditions) 1 mg of a given alpha-amylase will hydrolyze a certain amount of substrate and a blue colour will be produced. The colour intensity is measured at 620 nm. The measured absorbance is directly proportional to the specific activity (activity/mg of pure alpha-amylase protein) of the alpha-amylase in question under the given set of conditions.
Assay VII: Determination of Haloperoxidase Activity
An assay for determining haloperoxidase activity may be carried out by mixing 100 μΙ_ of haloperoxidase sample (containing about 0.2 pg enzyme protein/mL) and 100 pl_ of a 0.3 M sodium phosphate pH 7 buffer containing 0.5 M potassium bromide and 0.008% phenol red, adding the solution to 10 pL of 0.3% H2O2, and measuring the absorption at 595 nm as a function of time. Another assay using monochlorodimedone (Sigma M4632, ε = 20000 M-1cm-1 at 290 nm) as a substrate may be carried out by measuring the decrease in absorption at 290 nm as a function of time. The assay is performed in an aqueous solution of 0.1 M sodium phosphate or 0.1 M sodium acetate, 50 μΜ monochlorodimedone, 10 mM KBr/KCI, 1 mM H2O2 and about 1 pg/mL haloperoxidase.
Assay VIII: testing RNase activity RNase activity may be determined by fluorescence using fluorescence-quenched oligonucleotides probe. This probe emits signal after nuclease degradation according to the manual from the supplier (RNase alert kit, Integrated DNA Technology, Coralville, Iowa, USA). Briefly, RNase is diluted in water hardness 15°dH to obtain a concentration of 2 ppm, 5μΙ of the substrate was added to 95 μΙ of the RNase sample. Kinetic curve was measured for 10 min at 22°C using a Clariostar microplate reader (excitation 490nm, emission at 520nm).
Example 1
Preparation of swatches with biofilm
Swatches with biofilm of Brevundimonas sp. was pre-grown on Tryptone Soya Agar (TSA) (pH 7.3) (CM0131; Oxoid Ltd, Basingstoke, UK) for 2-5 days at 30°C. From a single colony, a loop-full was transferred to 10 mL of TSB and incubated for 1 day at 30°C with shaking (240 rpm). After propagation, cells were pelleted by centrifugation (Sigma Laboratory Centrifuge 6K15) (3000 g at 21 °C in 7 min) and resuspended in 10 mL of TSB diluted twice with water. Optical density (OD) at 600 nm was measured using a spectophometer (POLARstar Omega (BMG Labtech, Ortenberg, Germany). Fresh TSB diluted twice with water was inoculated to an OD600nm of 0.03, and 50 mL was added into a petridish (diameter 125 mm), in which a swatch (80 mm x 120 mm) of sterile cotton (WFK10A). After incubation (48 h at 15°C with shaking (100 rpm), swatches were rinsed twice with 0.9% (w/v) NaCI and dried in LAF bench for 60 min. Swatches were stored at 4°C prior to wash.
Wash experiment
Wash experiment was performed using the Automatic Mechanical Stress Assay (AMSA). With AMSA, the wash performance of many small volume enzyme-detergent solutions can be examined at the same time. The AMSA plate has many slots for test solutions, and a lid that firmly squeezes the textile to be washed against the slot openings. During the wash, the plate, test solutions, textile and lid are vigorously shaken to bring the test solution in contact with the textile and apply mechanical stress in a regular, periodic, oscillating manner.
The wash experiment was conducted under the experimental conditions specified below:
Model detergents and test materials were as follows:
For wash experiments, Model detergent A (containing 12% LAS, 11% AEO Biosoft N25-7 (Nl), 7% AEOS (SLES), 6% MPG, 3% ethanol, 3% TEA, 2.75% cocoa soap, 2.75% soya soap, 2% glycerol, 2% sodium hydroxide, 2% sodium citrate, 1% sodium formiate, 0.2% DTMPA and 0.2% PCA (all percentages are w/w)) (3.3 g/L) dissolved in water hardness 15°dH (Ca:Mg:NaHC03- = 4:1:1.5) was used. Soil was subsequently added to reach a concentration of 0.7 g soil/L (WFK09V pigment soil) to reveal biofilm. After washing, textiles were flushed in tap water and dried over night before scanning. Wash experiments were done twice.
Wash performance was measured as the brightness of the WFK09V pigment soiled, washed textile. Brightness can also be expressed as the intensity of the light reflected from the sample when illuminated with white light. When the sample is soiled, the intensity of the reflected light is lower, than that of a clean sample. Therefore, the intensity of the reflected light can be used to measure wash performance. Intensity measurements were made with a professional flatbed scanner (Kodak iQsmart, Kodak, Midtager 29, DK-2605 Brøndby, Denmark), which was used to capture an image of the washed and dried textile. To extract a value for the light intensity from the scanned images, 24-bit pixel values from the image were converted into values for red, green and blue (RGB). The intensity value (Int) was calculated by adding the RGB values together as vectors and then taking the length of the resulting vector:
Example 2
Wash synergy between DNase and second enzyme
To asses wash synergy between DNase and second enzyme, biofilm-harboring textile was AMSA washed a) in the absence of enzyme (blank), b) in the presence of DNase alone, c) in the presence of second enzyme alone and d) with a mixture of DNase and second enzyme. The resulting textile intensities and corresponding wash performances (WPs) are listed in Table 1 and 3. Wash performances attributable to DNase (WPDNase), second enzyme (WP2enz) and the mixture of the two (WPDNase +2enz) were quantified as the difference in intensity between textile washed
With and without enzyme. WPDNase — iDNase - iBIank, WP2enz— l2enz “ iBIank, WPDNase+2enz — lDNase+2enz “ I Blank- The synergistic component of wash performance WPsyn was quantified as the extent to which wash performance of mixed DNase and second enzyme (WPDNase+2enz) exceeded the sum of the individual wash performances of DNase alone and second enzyme alone: WPsyn = WPDNase +2enz — (WPDNase WP2enz)·
Below is shown synergistic effect two selected secondary enzymes Table 1. DNase and protease I
Table 2 DNase and cellulase

Claims (15)

1.First A cleaning composition comprising at least 0.0001 ppm DNase, at least 0.0001 ppm second enzyme and a cleaning component, wherein the cleaning component is selected from a.A cleaning composition comprising at least 0.0001 ppm DNase, at least 0.0001 ppm second enzyme and a cleaning component, while the cleaning component is selected from a. 0.1 to 15 wt% of at least one a surfactant; b.0.1 to 15 wt% of at least one surfactant; b. 0.5 to 20 wt% of at least one builder; and c.0.5 to 20 wt% of at least one builder; and c. 0.01 to 10 wt% of at least one bleach component.0.01 to 10 wt% of at least one bleach component. SEQUENCE LISTING <110> Novozymes A/S <120> CLEANING COMPOSITIONS AND USES THEREOF <130> 14531-DK-NP <160> 84 <170> Patentln version 3.5 <210> 1 <211> 182 <212> PRT <213> Bacillus sp-62451 <400> 1 Leu Pro Pro Asp Leu Pro Ser Lys Ser Thr Thr Gin Ala Gin Leu Asn 15 10 15 Ser Leu Asn Val Lys Asn Glu Glu Ser Met Ser Gly Tyr Ser Arg Glu 20 25 30 Lys Phe Pro His Trp lie Ser Gin Gly Asp Gly Cys Asp Thr Arg Gin 35 40 45 Val lie Leu Lys Arg Asp Ala Asp Asn Tyr Ser Gly Asn Cys Pro Val 50 55 60 Thr Ser Gly Lys Trp Tyr Ser Tyr Tyr Asp Gly He Thr Phe Asn Asp 65 70 75 80 Pro Ser Gin Leu Asp lie Asp His Val Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Ser Thr Ala Lys Arg Glu Asp Phe Ala 100 105 HO Asn Asp Leu Asn Gly Pro Gin Leu He Ala Val Ser Ala Ser Ser Asn Π5 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Ala 130 135 140 Gly Ala Asn Cys Ala Tyr Ala Lys Met Trp He Asn Thr Lys Tyr Asn 145 150 155 160 Trp Gly Leu His Leu Gin Ser Ser Glu Lys Thr Ala Leu Gin Gly Met 165 170 175 Leu Asn Ser Cys Ser Tyr 180 <210> 2 <211> 182 <212> PRT <213> Bacillus horikoshii <4 00> 2 Leu Pro Pro Gly Thr Pro Thr Lys Ser Glu Ala Gin Asn Gin Leu Asn 15 10 15 Ser Leu Thr Val Lys Ser Glu Gly Ser Met Thr Gly Tyr Ser Arg Asp 20 25 30 Leu Phe Pro His Trp Ser Gly Gin Gly Asn Gly Cys Asp Thr Arg Gin 35 40 45 Ile Val Leu Gin Arg Asp Ala Asp Tyr Tyr Thr Gly Thr Cys Pro Thr 50 55 60 Thr Ser Gly Lys Trp Tyr Ser Tyr Phe Asp Gly Val Ile Val Tyr Ser 65 70 75 80 Pro Ser Glu Ile Asp Ile Asp His Ile Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Glu Gin Arg Arg Ala Phe Ala 100 105 110 Asn Asp Leu Asn Gly Pro Gin Leu Ile Ala Val Thr Ala Ser Val Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Ala 130 135 140 Gly Ala Arg Cys Ala Tyr Ala Lys Trp Trp Ile Asn Thr Lys His Arg 145 150 155 160 Trp Asn Leu His Leu Gin Ser Ser Glu Lys Ser Ser Leu Gin Thr Met 165 170 175 Leu Asn Gly Cys Ala Tyr 180 <210> 3 <211> 182 <212> PRT <213> Bacillus sp-62520 <4 00> 3 Leu Pro Pro Gly Thr Pro Ser Lys Ser Glu Ala Gin Ser Gin Leu Asn 15 10 15 Ala Leu Thr Val Lys Pro Glu Asp Pro Met Thr Gly Tyr Ser Arg Asp 20 25 30 His Phe Pro His Trp Ile Ser Gin Gly Asn Gly Cys Asn Thr Arg Gin 35 40 45 Ile Val Leu Gin Arg Asp Ala Asp Tyr Tyr Ser Gly Ala Cys Pro Val 50 55 60 Thr Thr Gly Lys Trp Tyr Ser Tyr Phe Asp Gly Val Ile Val Tyr Ser 65 70 75 80 Pro Ser Glu Ile Asp Ile Asp His Ile Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Glu Lys Arg Arg Ser Phe Ala 100 105 110 Asn Asp Leu Asn Gly Pro Gin Leu Ile Ala Val Thr Ala Ser Val Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Ala 130 135 140 Gly Ala Arg Cys Ala Tyr Ala Lys Trp Trp Ile Asn Thr Lys His Arg 145 150 155 160 Trp Gly Leu His Leu Gin Ser Ser Glu Lys Ser Ser Leu Gin Ser Met 165 170 175 Leu Asn Gly Cys Ala Tyr 180 <210> 4 <211> 182 <212> PRT <213> Bacillus sp-62520 <4 00> 4 Leu Pro Pro Gly Thr Pro Ser Lys Ser Glu Ala Gin Ser Gin Leu Asn 15 10 15 Ala Leu Thr Val Lys Pro Glu Asp Pro Met Thr Gly Tyr Ser Arg Asp 20 25 30 His Phe Pro His Trp Ile Ser Gin Gly Asn Gly Cys Asn Thr Arg Gin 35 40 45 Ile Val Leu Gin Arg Asp Ala Asp Tyr Tyr Ser Gly Ala Cys Pro Val 50 55 60 Thr Thr Gly Lys Trp Tyr Ser Tyr Phe Asp Gly Val Ile Val Tyr Ser 65 70 75 80 Pro Ser Glu Ile Asp Ile Asp His Ile Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Glu Gin Arg Arg Ser Phe Ala 100 105 110 Asn Asp Leu Asn Gly Pro Gin Leu Ile Ala Val Thr Ala Ser Val Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Ala 130 135 140 Gly Ala Arg Cys Ala Tyr Ala Lys Trp Trp Ile Asn Thr Lys His Arg 145 150 155 160 Tirp Gly Leu His Leu Gin Ser Ser Glu Lys Ser Ser Leu Gin Ser Met 165 170 175 Leu Asn Gly Cys Ala Tyr 180 <210> 5 <211> 182 <212> PRT <213> Bacillus horikoshii <4 00> 5 Leu Pro Pro Gly Thr Pro Ser Lys Ser Glu Ala Gin Ser Gin Leu Asn 1 5 10 15 Ser Leu Thr Val Lys Ser Glu Asp Pro Met Thr Gly Tyr Ser Arg Asp 20 25 30 His Phe Pro His Trp Ser Gly Gin Gly Asn Gly Cys Asp Thr Arg Gin 35 40 45 Ile Val Leu Gin Arg Asp Ala Asp Tyr Tyr Ser Gly Asn Cys Pro Val 50 55 60 Thr Ser Gly Lys Trp Tyr Ser Tyr Phe Asp Gly Val Ile Val Tyr Ser 65 70 75 80 Pro Ser Glu Ile Asp Ile Asp His Val Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Glu Gin Arg Arg Ser Phe Ala 100 105 110 Asn Asp Leu Asn Gly Pro Gin Leu Ile Ala Val Thr Ala Ser Val Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Ala 130 135 140 Gly Ala Arg Cys Ala Tyr Ala Lys Trp Trp Ile Asn Thr Lys His Arg 145 150 155 160 Trp Asn Leu His Leu Gin Ser Ser Glu Lys Ser Ala Leu Gin Thr Met 165 170 175 Leu Asn Gly Cys Val Tyr 180 <210> 6 <211> 182 <212> PRT <213> Bacillus horikoshii <4 00> 6 Leu Pro Pro Gly Thr Pro Ser Lys Ser Glu Ala Gin Ser Gin Leu Asn 15 10 15 Ser Leu Thr Val Lys Thr Glu Asp Pro Met Thr Gly Tyr Ser Arg Asp 20 25 30 Leu Phe Pro His Trp Ser Gly Gin Gly Ser Gly Cys Asp Thr Arg Gin 35 40 45 Ile Val Leu Gin Arg Asp Ala Asp Tyr Phe Thr Gly Thr Cys Pro Thr 50 55 60 Thr Ser Gly Lys Trp Tyr Ser Tyr Phe Asp Gly Val lie Val Tyr Ser 65 70 75 80 Pro Ser Glu lie Asp Val Asp His lie Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Glu Gin Arg Arg Ala Phe Ala 100 105 110 Asn Asp Leu Thr Gly Pro Gin Leu lie Ala Val Thr Ala Ser Val Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Ala 130 135 140 Gly Ala Arg Cys Ala Tyr Ala Lys Trp Trp lie Asn Thr Lys His Arg 145 150 155 160 Trp Asn Leu His Leu Gin Ser Ser Glu Lys Ser Ser Leu Gin Thr Met 165 170 175 Leu Asn Gly Cys Ala Tyr 180 <210> 7 <211> 182 <212> PRT <213> Bacillus sp-16840 <4 00> 7 Leu Pro Pro Gly Thr Pro Ser Lys Ser Glu Ala Gin Ser Gin Leu Asn 15 10 15 Ala Leu Thr Val Lys Ala Glu Asp Pro Met Thr Gly Tyr Ser Arg Asn 20 25 30 Leu Phe Pro His Trp Asn Ser Gin Gly Asn Gly Cys Asn Thr Arg Gin 35 40 45 Leu Val Leu Gin Arg Asp Ala Asp Tyr Tyr Ser Gly Asn Cys Pro Val 50 55 60 Thr Ser Gly Arg Trp Tyr Ser Tyr Phe Asp Gly Val Val Val Thr Ser 65 70 75 80 Pro Ser Glu lie Asp lie Asp His lie Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Glu Lys Arg Lys Glu Phe Ala 100 105 110 Asn Asp Leu Asn Gly Pro Gin Leu lie Ala Val Thr Ala Ser Val Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Ala 130 135 140 Ala Ala Arg Cys Gly Tyr Ala Lys Trp Trp lie Asn Thr Lys Tyr Arg 145 150 155 160 Trp Asp Leu Ser Leu Gin Ser Ser Glu Lys Ser Ser Leu Gin Thr Met 165 170 175 Leu Asn Thr Cys Ser Tyr 180 <210> 8 <211> 182 <212> PRT <213> Bacillus sp-16840 <4 00> 8 Leu Pro Pro Gly Thr Pro Ser Lys Ser Gin Ala Gin Ser Gin Leu Asn 1 5 10 15 Ala Leu Thr Val Lys Ala Glu Asp Pro Met Thr Gly Tyr Ser Arg Asn 20 25 3o Leu Phe Pro His Trp Ser Ser Gin Gly Asn Gly Cys Asn Thr Arg Gin 35 40 45 Leu Val Leu Gin Arg Asp Ala Asp Tyr Tyr Ser Gly Asn Cys Pro Val 50 55 60 Thr Ser Gly Arg Trp Tyr Ser Tyr Phe Asp Gly Val Val Val Thr Ser 65 70 75 80 Pro Ser Glu lie Asp lie Asp His lie Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Glu Lys Arg Arg Glu Phe Ala 100 105 110 Asn Asp Leu Asn Gly Pro Gin Leu lie Ala Val Thr Ala Ser Val Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Val 130 135 140 Ala Ala Arg Cys Gly Tyr Ala Lys Trp Trp lie Asn Thr Lys Tyr Arg 145 150 155 160 Trp Asp Leu Ser Leu Gin Ser Ser Glu Lys Ser Ser Leu Gin Thr Met 165 170 175 Leu Asn Thr Cys Ser Tyr 180 <210> 9 <211> 182 <212> PRT <213> Bacillus sp-62668 <4 00> 9 Leu Pro Pro Gly Thr Pro Ser Lys Ser Glu Ala Gin Ser Gin Leu Thr 15 10 15 Ser Leu Thr Val Lys Pro Glu Asp Pro Met Thr Gly Tyr Ser Arg Asp 20 25 30 His Phe Pro His Trp lie Ser Gin Gly Asn Gly Cys Asn Thr Arg Gin 35 40 45 Ile Val Leu Gin Arg Asp Ala Asp Tyr Tyr Ser Gly Asn Cys Pro Val 50 55 60 Thr Thr Gly Lys Trp Tyr Ser Tyr Phe Asp Gly Val lie Val Tyr Ser 65 70 75 80 Pro Ser Glu lie Asp lie Asp His lie Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Ala Glu Gin Arg Arg Asn Phe Ala 100 105 110 Asn Asp Leu Asn Gly Pro Gin Leu lie Ala Val Thr Ala Ser Val Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Thr 130 135 140 Gly Ala Arg Cys Ala Tyr Ala Lys Trp Trp lie Asn Thr Lys Tyr Arg 145 150 155 160 Trp Gly Leu His Leu Gin Ser Ser Glu Lys Ser Ser Leu Gin Ser Met 165 170 175 Leu Asn Gly Cys Ala Tyr 180 <210> 10 <211> 183 <212> PRT <213> Bacillus sp-13395 <4 00> 10 Ala Phe Pro Pro Gly Thr Pro Ser Lys Ser Thr Ala Gin Ser Gin Leu 15 10 15 Asn Ser Leu Thr Val Lys Ser Glu Gly Ser Met Thr Gly Tyr Ser Arg 20 25 30 Asp Lys Phe Pro His Trp Ile Ser Gin Gly Asp Gly Cys Asp Thr Arg 35 40 45 Gin Leu Val Leu Lys Arg Asp Gly Asp Tyr Tyr Ser Gly Asn Cys Pro 50 55 60 Val Thr Ser Gly Lys Trp Tyr Ser Tyr Tyr Asp Gly Ile Ala Val Tyr 65 70 75 80 Ser Pro Ser Glu Ile Asp Ile Asp His Ile Val Pro Leu Ala Glu Ala 85 90 95 Trp Arg Ser Gly Ala Ser Gly Trp Thr Thr Glu Lys Arg Gin Asn Phe 100 105 110 Ala Asn Asp Leu Asn Gly Pro Gin Leu Ile Ala Val Thr Ala Ser Val 115 120 125 Asn Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg 130 135 140 Ser Gly Ser His Cys Ala Tyr Ala Lys Met Trp Val Asn Thr Lys Tyr 145 150 155 160 Arg Trp Gly Leu His Leu Gin Ser Ala Glu Lys Ser Ala Leu Gin Ser 165 170 175 Met Leu Asn Ala Cys Ser Tyr 180 < 210 > 11 <211> 185 <212> PRT <213> Bacillus horneckiae <4 00> 11 Ala Ser Ala Phe Pro Pro Gly Thr Pro Ser Lys Ser Thr Ala Gin Ser 15 10 15 Gin Leu Asn Ser Leu Thr Val Lys Ser Glu Gly Ser Met Thr Gly Tyr 20 25 30 Ser Arg Asp Lys Phe Pro His Trp Ile Ser Gin Gly Asp Gly Cys Asp 35 40 45 Thr Arg Gin Leu Val Leu Lys Arg Asp Gly Asp Tyr Tyr Ser Gly Asn 50 55 60 Cys Pro Val Thr Ser Gly Lys Trp Tyr Ser Tyr Tyr Asp Gly Ile Thr 65 70 75 80 Val Tyr Ser Pro Ser Glu Ile Asp Ile Asp His Ile Val Pro Leu Ala 85 90 95 Glu Ala Trp Arg Ser Gly Ala Ser Gly Trp Thr Thr Glu Lys Arg Gin 100 105 110 Ser Phe Ala Asn Asp Leu Asn Gly Pro Gin Leu Ile Ala Val Thr Ala 115 120 125 Ser Val Asn Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro 130 135 140 Pro Arg Ser Gly Ser His Cys Ala Tyr Ala Lys Met Trp Val Asn Thr 145 150 155 160 Lys Tyr Arg Trp Gly Leu His Val Gin Ser Ala Glu Lys Ser Ala Leu 165 170 175 Gin Ser Met Leu Asn Ala Cys Ser Tyr 180 185 <210> 12 <211> 182 <212> PRT <213> Bacillus sp-11238 <4 00> 12 Phe Pro Pro Glu Ile Pro Ser Lys Ser Thr Ala Gin Ser Gin Leu Asn 15 10 15 Ser Leu Thr Val Lys Ser Glu Asp Ala Met Thr Gly Tyr Ser Arg Asp 20 25 30 Lys Phe Pro His Trp Ile Ser Gin Gly Asp Gly Cys Asp Thr Arg Gin 35 40 45 Met Val Leu Lys Arg Asp Ala Asp Tyr Tyr Ser Gly Ser Cys Pro Val 50 55 60 Thr Ser Gly Lys Trp Tyr Ser Tyr Tyr Asp Gly Ile Thr Val Tyr Ser 65 70 75 80 Pro Ser Glu Ile Asp Ile Asp His Ile Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Glu Lys Arg Arg Asn Phe Ala 100 105 110 Asn Asp Leu Asn Gly Pro Gin Leu Ile Ala Val Thr Ala Ser Val Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Ser 130 135 140 Gly Ala Arg Cys Ala Tyr Ala Lys Met Trp Val Asn Thr Lys Tyr Arg 145 150 155 160 Trp Gly Leu His Leu Gin Ser Ala Glu Lys Ser Gly Leu Glu Ser Met 165 170 175 Leu Asn Thr Cys Ser Tyr 180 < 210 > 13 <211> 182 <212> PRT <213> Bacillus cibi <4 00> 13 Thr Pro Pro Gly Thr Pro Ser Lys Ser Ala Ala Gin Ser Gin Leu Asn 15 10 15 Ala Leu Thr Val Lys Thr Glu Gly Ser Met Ser Gly Tyr Ser Arg Asp 20 25 30 Leu Phe Pro His Trp Ile Ser Gin Gly Ser Gly Cys Asp Thr Arg Gin 35 40 45 Val Val Leu Lys Arg Asp Ala Asp Ser Tyr Ser Gly Asn Cys Pro Val 50 55 60 Thr Ser Gly Ser Trp Tyr Ser Tyr Tyr Asp Gly Val Thr Phe Thr Asn 65 70 75 80 Pro Ser Asp Leu Asp Ile Asp His Ile Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Ser Lys Arg Gin Asp Phe Ala 100 105 110 Asn Asp Leu Ser Gly Pro Gin Leu Ile Ala Val Ser Ala Ser Thr Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Ser 130 135 140 Gly Ala Ala Cys Gly Tyr Ser Lys Trp Trp Ile Ser Thr Lys Tyr Lys 145 150 155 160 Trp Gly Leu Ser Leu Gin Ser Ser Glu Lys Thr Ala Leu Gin Gly Met 165 170 175 Leu Asn Ser Cys Ser Tyr 180 <210> 14 <211> 182 <212> PRT <213> Bacillus sp-18318 <4 00> 14 Phe Pro Pro Gly Thr Pro Ser Lys Ser Thr Ala Gin Ser Gin Leu Asn 15 10 15 Ser Leu Thr Val Lys Ser Glu Gly Ser Met Thr Gly Tyr Ser Arg Asp 20 25 30 Lys Phe Pro His Trp Ile Gly Gin Gly Ser Gly Cys Asp Thr Arg Gin 35 40 45 Leu Val Leu Gin Arg Asp Ala Asp Tyr Tyr Ser Gly Ser Cys Pro Val 50 55 60 Thr Ser Gly Lys Trp Tyr Ser Tyr Tyr Asp Gly Val Thr Phe Tyr Asp 65 70 75 80 Pro Ser Asp Leu Asp Ile Asp His Val Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Ser Thr Gin Lys Arg Lys Asp Phe Ala 100 105 110 Asn Asp Leu Ser Gly Pro Gin Leu Ile Ala Val Ser Ala Ser Ser Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Thr Arg Ser 130 135 140 Gly Ala Ala Cys Gly Tyr Ser Lys Trp Trp Ile Ser Thr Lys His Lys 145 150 155 160 Trp Gly Leu Ser Leu Gin Ser Ser Glu Lys Asn Ala Leu Gin Gly Met 165 170 175 Leu Asn Ser Cys Val Tyr 180 <210> 15 <211> 182 <212> PRT <213> Bacillus idriensis <4 00> 15 Leu Pro Pro Gly Thr Pro Ser Lys Ser Thr Ala Gin Ser Gin Leu Asn 15 10 15 Ala Leu Thr Val Gin Thr Glu Gly Ser Met Thr Gly Tyr Ser Arg Asp 20 25 30 Lys Phe Pro His Trp Ile Ser Gin Gly Asn Gly Cys Asp Thr Arg Gin 35 40 45 Val Val Leu Gin Arg Asp Ala Asp Tyr Tyr Ser Gly Thr Cys Pro Val 50 55 60 Thr Ser Gly Lys Trp Tyr Ser Tyr Tyr Asp Gly Val Thr Leu Tyr Asn 65 70 75 80 Pro Ser Asp Leu Asp Ile Asp His Val Val Ala Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Asp Lys Arg Glu Asp Phe Ala 100 105 110 Asn Asp Leu Ser Gly Thr Gin Leu Ile Ala Val Ser Ala Ser Thr Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Ser 130 135 140 Gly Ala Ala Cys Gly Tyr Ala Lys Trp Trp Ile Ser Thr Lys Tyr Lys 145 150 155 160 Trp Asn Leu Asn Leu Gin Ser Ser Glu Lys Thr Ala Leu Gin Ser Met 165 170 175 Leu Asn Ser Cys Ser Tyr 180 <210> 16 <211> 182 <212> PRT <213> Bacillus algicola <400> 16 Phe Pro Pro Gly Thr Pro Ser Lys Ser Glu Ala Gin Ser Gin Leu Asn 1 5 10 15 Ser Leu Thr Val Gin Ser Glu Gly Ser Met Ser Gly Tyr Ser Ara Asd 20 25 30 Lys Phe Pro His Trp Ile Gly Gin Gly Asn Gly Cys Asp Thr Arq Gin 35 40 45 Leu Val Leu Gin Arg Asp Ala Asp Tyr Tyr Ser Gly Asp Cys Pro Val 50 55 60 Thr Ser Gly Lys Trp Tyr Ser Tyr Phe Asp Gly Val Thr Val Tyr Asp 65 70 75 80 Pro Ser Asp Leu Asp Ile Asp His Met Val Pro Met Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Ser Thr Gin Lys Arg Glu Asp Phe Ala 100 105 110 Asn Asp Leu Ser Gly Pro His Leu Ile Ala Val Thr Ala Ser Ser Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Lys Pro Thr Arg Tyr 130 135 140 Gly Ala His Cys Gly Tyr Ala Lys Trp Trp Ile Asn Thr Lys Tyr Val 145 150 155 160 Tyr Asp Leu Thr Leu Gin Ser Ser Glu Lys Thr Glu Leu Gin Ser Met 165 170 175 Leu Asn Thr Cys Ser Tyr 180 <210> 17 <211> 182 <212> PRT <213> Enviromental sample J < 4 O O > 17 Leu Pro Pro Asn Ile Pro Ser Lys Ala Asp Ala Leu Thr Lys Leu Asn 15 10 15 Ala Leu Thr Val Gin Thr Glu Gly Pro Met Thr Gly Tyr Ser Arg Asp 20 25 30 Leu Phe Pro His Trp Ser Ser Gin Gly Asn Gly Cys Asn Thr Arg His 35 40 45 Val Val Leu Lys Arg Asp Ala Asp Ser Val Val Asp Thr Cys Pro Val 50 55 60 Thr Thr Gly Arg Trp Tyr Ser Tyr Tyr Asp Gly Leu Val Phe Thr Ser 65 70 75 80 Ala Ser Asp Ile Asp Ile Asp His Val Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Ser Thr Lys Arg Gin Ser Phe Ala 100 105 110 Asn Asp Leu Asn Gly Pro Gin Leu Ile Ala Val Ser Ala Thr Ser Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Ala 130 135 140 Gly Ala Arg Cys Ala Tyr Ala Lys Met Trp Val Glu Thr Lys Ser Arg 145 150 155 160 Trp Gly Leu Thr Leu Gin Ser Ser Glu Lys Ala Ala Leu Gin Thr Ala 165 170 175 Ile Asn Ala Cys Ser Tyr 180 <210> 18 <211> 182 <212> PRT <213> Bacillus vietnamensis < 4 O O > 18 Phe Pro Pro Gly Thr Pro Ser Lys Ser Thr Ala Gin Ser Gin Leu Asn 15 10 15 Ala Leu Thr Val Lys Ser Glu Ser Ser Met Thr Gly Tyr Ser Arg Asp 20 25 30 Lys Phe Pro His Trp Ile Gly Gin Arg Asn Gly Cys Asp Thr Arg Gin 35 40 45 Leu Val Leu Gin Arg Asp Ala Asp Ser Tyr Ser Gly Ser Cys Pro Val 50 55 60 Thr Ser Gly Ser Trp Tyr Ser Tyr Tyr Asp Gly Val Thr Phe Thr Asp 65 70 75 80 Pro Ser Asp Leu Asp Ile Asp His Val Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Ala Lys Arg Glu Asp Phe Ala 100 105 110 Asn Asp Leu Ser Gly Pro Gin Leu Ile Ala Val Ser Ala Ser Ser Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Ser 130 135 140 Gly Ala Ala Cys Gly Tyr Ser Lys Trp Trp Ile Ser Thr Lys Tyr Lys 145 150 155 160 Trp Gly Leu Ser Leu Gin Ser Ser Glu Lys Thr Ala Leu Gin Gly Met 165 170 175 Leu Asn Ser Cys Ile Tyr 180 <210> 19 <211> 182 <212> PRT <213> Bacillus hwajinpoensis <4 00> 19 Ile Pro Pro Gly Thr Pro Ser Lys Ser Ala Ala Gin Ser Gin Leu Asp 15 10 15 Ser Leu Ala Val Gin Ser Glu Gly Ser Met Ser Gly Tyr Ser Arcr Asp 20 25 3o Lys Phe Pro His Trp He Gly Gin Gly Asn Gly Cys Asp Thr Arq Gin 35 40 45 Leu Val Leu Gin Arg Asp Ala Asp Tyr Tyr Ser Gly Asp Cys Pro Val 50 55 60 Thr Ser Gly Lys Trp Tyr Ser Tyr Phe Asp Gly Val Gin Val Tyr Asp 65 70 75 80 Pro Ser Tyr Leu Asp lie Asp His Met Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Ser Thr Gin Lys Arg Glu Asp Phe Ala 100 105 110 Asn Asp Leu Asp Gly Pro His Leu He Ala Val Thr Ala Ser Ser Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Lys Pro Thr Arg Tyr 130 135 140 Ser Ala His Cys Gly Tyr Ala Lys Trp Trp He Asn Thr Lys Tyr Val 145 150 155 160 Tyr Asp Leu Asn Leu Gin Ser Ser Glu Lys Ser Ala Leu Gin Ser Met 165 170 175 Leu Asn Thr Cys Ser Tyr 180 <210> 20 <211> 182 <212> PRT <213> Paenibacillus mucilaginosus <4 00> 20 Leu Pro Pro Gly Thr Pro Ser Lys Ser Thr Ala Gin Ser Gin Leu Asn 15 10 15 Ser Leu Thr Val Lys Ser Glu Ser Thr Met Thr Gly Tyr Ser Arg Asp 20 25 30 Lys Phe Pro His Trp Thr Ser Gin Gly Gly Gly Cys Asp Thr Arg Gin 35 40 45 Val Val Leu Lys Arg Asp Ala Asp Tyr Tyr Ser Gly Ser Cys Pro Val 50 55 60 Thr Ser Gly Lys Trp Tyr Ser Tyr Tyr Asp Gly lie Thr Val Tyr Ser 65 70 75 80 Pro Ser Glu lie Asp lie Asp His lie Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Glu Lys Arg Gin Asn Phe Ala 100 105 110 Asn Asp Leu Gly Gly Pro Gin Leu lie Ala Val Thr Ala Ser Ser Asn 115 120 125 Arg Ala Lys Gly Asp Gin Asp Pro Ser Thr Trp Lys Pro Thr Arg Ser 130 135 140 Gly Ala His Cys Ala Tyr Ala Lys Trp Trp lie Asn Thr Lys Tyr Arg 145 150 155 160 Trp Gly Leu His Leu Gin Ser Ser Glu Lys Thr Ala Leu Gin Ser Met 165 170 175 Leu Asn Thr Cys Ser Tyr 180 <210> 21 <211> 182 <212> PRT <213> Bacillus indicus <4 00> 21 Thr Pro Pro Gly Thr Pro Ser Lys Ser Thr Ala Gin Thr Gin Leu Asn 15 10 15 Ala Leu Thr Val Lys Thr Glu Gly Ser Met Thr Gly Tyr Ser Arg Asp 20 25 30 Leu Phe Pro His Trp lie Ser Gin Gly Ser Gly Cys Asp Thr Arg Gin 35 40 45 Val Val Leu Lys Arg Asp Ala Asp Tyr Tyr Ser Gly Ser Cys Pro Val 50 55 60 Thr Ser Gly Lys Trp Tyr Ser Tyr Tyr Asp Gly Val Thr Phe Tyr Asp 65 70 75 80 Pro Ser Asp Leu Asp lie Asp His lie Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Ser Lys Arg Gin Asp Phe Ala 100 105 110 Asn Asp Leu Ser Gly Pro Gin Leu lie Ala Val Ser Ala Ser Thr Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Ala 130 135 140 Gly Ala Ala Cys Gly Tyr Ser Lys Trp Trp lie Ser Thr Lys Tyr Lys 145 150 155 160 Trp Gly Leu Ser Leu Gin Ser Ser Glu Lys Thr Ala Leu Gin Gly Met 165 170 175 Leu Asn Ser Cys Ser Tyr 180 <210> 22 <211> 182 <212> PRT <213> Bacillus marisflavi <4 00> 22 Thr Pro Pro Val Thr Pro Ser Lys Ala Thr Ser Gin Ser Gin Leu Asn 15 10 15 Gly Leu Thr Val Lys Thr Glu Gly Ala Met Thr Gly Tyr Ser Arg Asp 20 25 30 Lys Phe Pro His Trp Ser Ser Gin Gly Gly Gly Cys Asp Thr Arg Gin 35 40 45 Val Val Leu Lys Arg Asp Ala Asp Ser Tyr Ser Gly Asn Cys Pro Val 50 55 60 Thr Ser Gly Ser Trp Tyr Ser Tyr Tyr Asp Gly Val Lys Phe Thr Asn 65 70 75 80 Pro Ser Asp Leu Asp Ile Asp His Ile Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Ala Gin Arg Glu Ala Phe Ala 100 105 110 Asn Asp Leu Ser Gly Ser Gin Leu Ile Ala Val Ser Ala Ser Ser Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Ala 130 135 140 Gly Ala Lys Cys Gly Tyr Ala Lys Trp Trp Ile Ser Thr Lys Ser Lys 145 150 155 160 Trp Asn Leu Ser Leu Gin Ser Ser Glu Lys Thr Ala Leu Gin Gly Met 165 170 175 Leu Asn Ser Cys Val Tyr 180 <210> 23 <211> 184 <212> PRT <213> Bacillus luciferensis <4 00> 23 Ala Ser Leu Pro Pro Gly Ile Pro Ser Leu Ser Thr Ala Gin Ser Gin 15 10 15 Leu Asn Ser Leu Thr Val Lys Ser Glu Gly Ser Leu Thr Gly Tyr Ser 20 25 30 Arg Asp Val Phe Pro His Trp Ile Ser Gin Gly Ser Gly Cys Asp Thr 35 40 45 Arg Gin Val Val Leu Lys Arg Asp Ala Asp Tyr Tyr Ser Gly Asn Cys 50 55 60 Pro Val Thr Ser Gly Lys Trp Tyr Ser Tyr Tyr Asp Gly Val Thr Val 65 70 75 80 Tyr Ser Pro Ser Glu Ile Asp Ile Asp His Val Val Pro Leu Ala Glu 85 90 95 Ala Trp Arg Ser Gly Ala Ser Ser Trp Thr Thr Glu Lys Arg Gin Asn 100 105 110 Phe Ala Asn Asp Leu Asn Gly Pro Gin Leu Ile Ala Val Thr Ala Ser 115 120 125 Ser Asn Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Thr 130 135 140 Arg Thr Gly Ala Arg Cys Ala Tyr Ala Lys Met Trp Ile Asn Thr Lys 145 150 155 160 Tyr Arg Trp Gly Leu His Leu Gin Ser Ser Glu Lys Ser Ala Leu Gin 165 170 175 Ser Met Leu Asn Thr Cys Ser Tyr 180 <210> 24 <211> 182 <212> PRT <213> Bacillus marisflavi <4 00> 24 Thr Pro Pro Val Thr Pro Ser Lys Glu Thr Ser Gin Ser Gin Leu Asn 15 10 15 Gly Leu Thr Val Lys Thr Glu Gly Ala Met Thr Gly Tyr Ser Arg Asp 20 25 30 Lys Phe Pro His Trp Ser Ser Gin Gly Gly Gly Cys Asp Thr Arg Gin 35 40 45 Val Val Leu Lys Arg Asp Ala Asp Ser Tyr Ser Gly Asn Cys Pro Val 50 55 60 Thr Ser Gly Ser Trp Tyr Ser Tyr Tyr Asp Gly Val Lys Phe Thr His 65 70 75 80 Pro Ser Asp Leu Asp Ile Asp His Ile Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Ala Gin Arg Glu Ala Phe Ala 100 105 110 Asn Asp Leu Ser Gly Ser Gin Leu Ile Ala Val Ser Ala Ser Ser Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Ala 130 135 140 Gly Ala Lys Cys Gly Tyr Ala Lys Trp Trp Ile Ser Thr Lys Ser Lys 145 150 155 160 Trp Asn Leu Ser Leu Gin Ser Ser Glu Lys Thr Ala Leu Gin Gly Met 165 170 175 Leu Asn Ser Cys Val Tyr 180 <210> 25 <211> 182 <212> PRT <213> Bacillus sp.SEQUENCE LISTING <110> Novozymes A / S <120> CLEANING COMPOSITIONS AND USES THEREOF <130> 14531-DK-NP <160> 84 <170> Patent Version 3.5 <210> 1 <211> 182 <212> PRT <213> Bacillus sp-62451 <400> 1 Leu Pro Pro Asp Leu Pro Ser List Ser Thr Thr Gin Ala Gin Leu Asn 15 10 15 Ser Leu Asn Val Lys Asn Glu Glu Ser With Ser Gly Tyr Ser Arg Glu 20 25 30 Lys Phe Pro His Trp lie Ser Gin Gly Asp Gly Cys Asp Thr Arg Gin 35 40 45 Val lie Leu Lys Arg Asp Ala Asp Asn Tyr Ser Gly Asn Cys Pro Val 50 55 60 Thr Ser Gly Lys Trp Tyr Ser Tyr Tyr Asp Gly He Thr Phe Asn Asp 65 70 75 80 Pro Ser Gin Leu Asp lie Asp His Val Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Ser Thr Ala Lys Arg Glu Asp Phe Ala 100 105 HO Asn Asp Leu Asn Gly Pro Gin Leu He Ala Val Ser Ala Ser Ser Asn 1205 120 125 Arg Ser List Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Ala 130 135 140 Gly Ala Asn Cys Ala Tyr Ala Lys With Trp He Asn Thr List Tyr Asn 145 150 155 160 Trp Gly Leu His Leu Gin Ser Ser Glu Lys Thr Ala Leu Gin Gly With 165 170 175 Leu Asn Ser Cys Ser Tyr 180 <210> 2 <211> 182 <212> PRT <213> Bacillus horikoshii <4 00> 2 Leu Pro Pro Gly Thr Pro Thr Lys Ser Glu Ala Gin Asn Gin Leu Asn 15 10 15 Ser Leu Thr Val Lys Ser Glu Gly Ser With Thr Gly Tyr Ser Arg Asp 20 25 30 Leu Phe Pro His Trp Ser Gly Gin Gly Asn Gly Cys Asp Thr Arg Gin 35 40 45 Ile Val Leu Gin Arg Asp Ala Asp Tyr Tyr Thr Gly Thr Cys Pro Thr 50 55 60 Thr Ser Gly Lys Trp Tyr Ser Tyr Phe Asp Gly Val Ile Val Tyr Ser 65 70 75 80 Pro Ser Glu Ile Asp Ile Asp His Ile Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Glu Gin Arg Arg Ala Phe Ala 100 105 110 Asn Asp Leu Asn Gly Pro Gin Leu Ile Ala Val Thr Ala Ser Val Asn 115 120 125 Arg Ser List Gly Asp Gin Asp Pro Ser Thr Trin Gin Pro Pro Arg Ala 130 135 140 Gly Ala Arg Cys Ala Tyr Ala Lys Trp Trp Ile Asn Thr Lys His Arg 145 150 155 160 Trp Asn Leu His Leu Gin Ser Ser Glu Lys Ser Ser Leu Gin Thr Met 165 170 175 Leu Asn Gly Cys Ala Tyr 180 <210> 3 <211> 182 <212> PRT <213> Bacil loop sp-62520 <4 00> 3 Leu Pro Pro Gly Thr Pro Ser List Ser Glu Ala Gin Ser Gin Leu Asn 15 10 15 Ala Leu Thr Val List Pro Glu Asp Pro With Thr Gly Tyr Ser Arg Asp 20 25 30 His Phe Pro His Trp Ile Ser Gin Gly Asn Gly Cys Asn Thr Arg Gin 35 40 45 Ile Val Leu Gin Arg Asp Ala Asp Tyr Tyr Ser Gly Ala Cys Pro Val 50 55 60 Thr Thr Gly Lys Trp Tyr Ser Tyr Phe Asp Gly Val Ile Val Tyr Ser 65 70 75 80 Pro Ser Glu Ile Asp Ile Asp His Ile Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Glu Lys Arg Arg Ser Phe Ala 100 105 110 Asn Asp Leu Asn Gly Pro Gin Leu Ile Ala Val Thr Ala Ser Val Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Ala 130 135 140 Gly Ala Arg Cys Ala Tyr Ala Lys Trp Trp Ile Asn Thr Lys His Arg 145 150 155 160 Trp Gly Leu His Leu Gin Ser Ser Glu Lys Ser Ser Leu Gin Ser Met 165 170 175 Leu Asn Gly Cys Ala Tyr 180 <210> 4 <211> 182 <212> PRT <213> Bacillus sp-62520 <400> 4 Leu Pro Pro Gly Thr Pro Ser Lys Ser Glu Ala Gin Ser Gin Leu Asn 15 10 15 Ala Leu Thr Val List Pro Glu Asp Pro With Thr Gly Tyr Ser Arg Asp 20 25 30 His Phe Pro His Trp Ile Ser Gin Gly Asn Gly Cys Asn Thr Arg Gin 35 40 45 Ile Val Leu Gin Arg Asp Ala Asp Tyr Tyr Ser Gly Ala Cys Pro Val 50 55 60 Thr Thr Gly Lys Trp Tyr Ser Tyr Phe Asp Gly Val Ile Val Tyr Ser 65 70 75 80 Pro Ser Glu Ile Asp Ile Asp His Ile Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Gin Arg Arg Ser Phe Ala 100 105 110 Asn Asp Leu Asn Gly Pro Gin Leu Ile Ala Val Thr Ala Ser Val Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Ala 130 135 140 Gly Ala Arg Cys Ala Tyr Ala Lys Trp Trp Ile Asn Thr Lys His Arg 145 150 155 160 Tirp Gly Leu His Leu Gin Ser Ser Glu Lys Ser Ser Leu Gin Ser Met 165 170 175 Leu Asn Gly Cys Ala Tyr 180 <210> 5 <211> 182 <212> PRT <213> Bacillus horikoshii <4 00> 5 Leu Pro Pro Gly Thr Pro Ser Lys Ser Glu Ala Gin Ser Gin Leu Asn 1 5 10 15 Ser Leu Thr Val Lys Ser Glu Asp Pro With Thr Gly Tyr Ser Arg Asp 20 25 30 His Phe Pro His Trp Ser Gly Gin Gly Asn Gly Cys Asp Thr Arg Gin 35 40 45 Ile Val Leu Gin Arg Asp Ala Asp Tyr Tyr Ser Gly Asn Cys Pro Val 50 55 60 Thr Ser Gly Lys Trp Tyr Ser Tyr Phe Asp Gly Val Ile Val Tyr Ser 65 70 75 80 Pro Ser Glu Ile Asp Ile Asp His Val Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Glu Gin Arg Arg Ser Phe Ala 100 105 110 Asn Asp Leu Asn Gly Pro Gin Leu Ile Ala Val Thr Ala Ser Val Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Ala 130 135 140 Gly Ala Arg Cys Ala Tyr Ala Lys Trp Trp Ile Asn Thr Lys His Arg 145 150 155 160 Trp Asn Leu His Leu Gin Ser Ser Glu Lys Ser Ala Leu Gin Thr Met 165 170 175 Leu Asn Gly Cys Val Tyr 180 <210> 6 <211> 182 <212> PRT <213> Bacillus horikoshii <4 00 > 6 Leu Pro Pro Gly Thr Pro Ser Lys Ser Glu Ala Gin Ser Gin Leu Asn 15 10 15 Ser Leu Thr Val Lys Thr Glu Asp Pro With Thr Gly Tyr Ser Arg Asp 20 25 30 Leu Phe Pro His Trp Ser Gly Gin Gly Ser Gly Cys Asp Thr Arg Gin 35 40 45 Ile Val Leu Gin Arg Asp Ala Asp Tyr Phe Thr Gly Thr Cys Pro Thr 50 55 60 Thr Ser Gly Lys Trp Tyr Ser Tyr Phe Asp Gly Val lie Val Tyr Ser 65 70 75 80 Pro Ser Glu lie Asp Val Asp His lie Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Glu Gin Arg Arg Ala Phe Ala 100 105 110 Asn Asp Leu Thr Gly Pro Gin Leu lie Ala Val Thr Ala Ser Val Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Ala 130 135 140 Gly Ala Arg Cys Ala Tyr Ala Lys Trp Trp lie Asn Thr Lys His Arg 145 150 155 160 Trp Asn Leu His Leu Gin Ser Ser Glu Lys Ser Ser Leu Gin Thr Met 165 170 175 Leu Asn Gly Cys Ala Tyr 180 <210> 7 <211> 182 <212> PRT <213> Bacillus sp-16840 <4 00> 7 Leu Pro Pro Gly Thr Pro Ser Lys Ser Glu Ala Gin Ser Gin Leu Asn 15 10 15 Ala Leu Thr Val List Ala Glu Asp Pro With Thr Gly Tyr Ser Arg Asn 20 25 30 Leu Phe Pro His Trp Asn Ser Gin Gly Asn Gly Cys Asn Thr Arg Gin 35 40 45 Leu Val Leu Gin Arg Asp Ala Asp Tyr Tyr Ser Gly Asn Cys Pro Val 50 55 60 Thr Ser Gly Arg Trp Tyr Ser Tyr Phe Asp Gly Val Val Val Thr Ser 65 70 75 80 Pro Ser Glu lie Asp lie Asp His lie Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Glu Lys Arg Lys Glu Phe Ala 100 105 110 Asn Asp Leu Asn Gly Pro Gin Leu lie Ala Val Thr Ala Ser Val Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Ala 130 135 140 Ala Ala Arg Cys Gly Tyr Ala Lys Trp Trp lie Asn Thr Lys Tyr Arg 145 150 155 160 Trp Asp Leu Ser Leu Gin Ser Ser Glu Lys Ser Ser Leu Gin Thr Met 165 170 175 Leu Asn Thr Cys Ser Tyr 180 <210> 8 <211> 182 <212> PRT <213 > Bacillus sp-16840 <4 00> 8 Leu Pro Pro Gly Thr Pro Ser Lys Ser Gin Ala Gin Ser Gin Leu Asn 1 5 10 15 Ala Leu Thr Val Lys Ala Glu Asp Pro With Thr Gly Tyr Ser Arg Asn 20 25 3o Leu Phe Pro His Trp Ser Ser Gly Gly Asn Gly Cys Asn Thr Arg Gin 35 40 45 Leu Val Leu Gin Arg Asp Ala Asp Tyr Tyr Ser Gly Asn Cys Pro Val 50 55 60 Thr Ser Gly Arg Trp Tyr Ser Tyr Phe Asp Gly Val Val Val Thr Ser 65 70 75 80 Pro Ser Glu lie Asp lie Asp His l ie Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Glu List Arg Arg Glu Phe Ala 100 105 110 Asn Asp Leu Asn Gly Pro Gin Leu lie Ala Val Thr Ala Ser Val Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Val 130 135 140 Ala Ala Arg Cys Gly Tyr Ala Lys Trp Trp lie Asn Thr Lys Tyr Arg 145 150 155 160 Trp Asp Leu Ser Leu Gin Ser Ser Glu Lys Ser Ser Leu Gin Thr Met 165 170 175 Leu Asn Thr Cys Ser Tyr 180 <210> 9 <211> 182 <212> PRT <213> Bacillus sp-62668 <4 00> 9 Leu Pro Pro Gly Thr Pro Ser Lys Ser Glu Ala Gin Ser Gin Leu Thr 15 10 15 Ser Leu Thr Val List Pro Glu Asp Pro With Thr Gly Tyr Ser Arg Asp 20 25 30 His Phe Pro His Trp lie Ser Gin Gly Asn Gly Cys Asn Thr Arg Gin 35 40 45 Ile Val Leu Gin Arg Asp Ala Asp Tyr Tyr Ser Gly Asn Cys Pro Val 50 55 60 Thr Thr Gly Lys Trp Tyr Ser Tyr Phe Asp Gly Val lie Val Tyr Ser 65 70 75 80 Pro Ser Glu lie Asp lie Asp His lie Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Ala Glu Gin Ar g Arg Asn Phe Ala 100 105 110 Asn Asp Leu Asn Gly Pro Gin Leu lie Ala Val Thr Ala Ser Val Asn 115 120 125 Arg Ser List Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Thr 130 135 140 Gly Ala Arg Cys Ala Tyr Ala Lys Trp Trp lie Asn Thr Lys Tyr Arg 145 150 155 160 Trp Gly Leu His Leu Gin Ser Ser Glu Lys Ser Ser Leu Gin Ser Met 165 170 175 Leu Asn Gly Cys Ala Tyr 180 <210> 10 <211> 183 <212> PRT <213> Bacillus sp-13395 <400> 10 Ala Phe Pro Pro Gly Thr Pro Ser Lys Ser Thr Ala Gin Ser Gin Leu 15 10 15 Asn Ser Leu Thr Val Lys Ser Glu Gly Ser With Thr Gly Tyr Ser Arg 20 25 30 Asp Lys Phe Pro His Trp Ile Ser Gin Gly Asp Gly Cys Asp Thr Arg 35 40 45 Gin Leu Val Leu Lys Arg Asp Gly Asp Tyr Tyr Ser Gly Asn Cys Pro 50 55 60 Val Thr Ser Gly Lys Trp Tyr Ser Tyr Tyr Asp Gly Ile Ala Val Tyr 65 70 75 80 Ser Pro Ser Glu Ile Asp Ile Asp His Ile Val Pro Leu Ala Glu Ala 85 90 95 Trp Arg Ser Gly Ala Ser Gly Trp Thr Thr Glu Lys Arg Gin Asn Phe 100 105 110 Ala Asn Asp Leu Asn Gly Pro Gin Leu Ile Ala Val Thr Al a Ser Val 115 120 125 Asn Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg 130 135 140 Ser Gly Ser His Cys Ala Tyr Ala Lys With Trp Val Asn Thr Lys Tyr 145 150 155 160 Arg Trp Gly Leu His Leu Gin Ser Ala Glu List Ser Ala Leu Gin Ser 165 170 175 With Leu Asn Ala Cys Ser Tyr 180 <210> 11 <211> 185 <212> PRT <213> Bacillus horneckiae <4 00> 11 Ala Ser Ala Phe Pro Pro Gly Thr Pro Ser Lys Ser Thr Ala Gin Ser 15 10 15 Gin Leu Asn Ser Leu Thr Val Lys Ser Glu Gly Ser With Thr Gly Tyr 20 25 30 Ser Arg Asp Lys Phe Pro His Trp Ile Ser Gin Gly Asp Gly Cys Asp 35 40 45 Thr Arg Gin Leu Val Leu Lys Arg Asp Gly Asp Tyr Tyr Ser Gly Asn 50 55 60 Cys Pro Val Thr Ser Gly Lys Trp Tyr Ser Tyr Tyr Asp Gly Ile Thr 65 70 75 80 Val Tyr Ser Pro Ser Glu Ile Asp Ile Asp His Ile Val Pro Leu Ala 85 90 95 Glu Ala Trp Arg Ser Gly Ala Ser Gly Trp Thr Thr Glu Lys Arg Gin 100 105 110 Ser Phe Ala Asn Asp Leu Asn Gly Pro Gin Leu Ile Ala Val Thr Ala 115 120 125 Ser Val Asn Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Tr p Gin Pro 130 135 140 Pro Arg Ser Gly Ser His Cys Ala Tyr Ala Lys With Trp Val Asn Thr 145 150 155 160 Lys Tyr Arg Trp Gly Leu His Val Gin Ser Ala Glu Lys Ser Ala Leu 165 170 175 Gin Ser With Leu Asn Ala Cys Ser Tyr 180 185 <210> 12 <211> 182 <212> PRT <213> Bacillus sp-11238 <400> 12 Phe Pro Pro Glu Ile Pro Ser Lys Ser Thr Ala Gin Ser Gin Leu Asn 15 10 15 Ser Leu Thr Val Lys Ser Glu Asp Ala With Thr Gly Tyr Ser Arg Asp 20 25 30 Lys Phe Pro His Trp Ile Ser Gin Gly Asp Gly Cys Asp Thr Arg Gin 35 40 45 With Val Leu Lys Arg Asp Ala Asp Tyr Tyr Ser Gly Ser Cys Pro Val 50 55 60 Thr Ser Gly Light Trp Tyr Ser Tyr Tyr Asp Gly Ile Thr Val Tyr Ser 65 70 75 80 Pro Ser Glu Ile Asp Ile Asp His Ile Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Glu Lys Arg Arg Asn Phe Ala 100 105 110 Asn Asp Leu Asn Gly Pro Gin Leu Ile Ala Val Thr Ala Ser Val Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Tr Gin Pro Pro Arg Ser 130 135 140 Gly Ala Arg Cys Ala Tyr Ala List With Trp Val As n Thr Lys Tyr Arg 145 150 155 160 Trp Gly Leu His Leu Gin Ser Ala Glu Lys Ser Gly Leu Glu Ser With 165 170 175 Leu Asn Thr Cys Ser Tyr 180 <210> 13 <211> 182 <212> PRT <213> Bacillus cibi <4 00> 13 Thr Pro Pro Gly Thr Pro Ser Lys Ser Ala Ala Gin Ser Gin Leu Asn 15 10 15 Ala Leu Thr Val Lys Thr Glu Gly Ser Ser Ser Gly Tyr Ser Arg Asp 20 25 30 Leu Phe Pro His Trp Ile Ser Gin Gly Ser Gly Cys Asp Thr Arg Gin 35 40 45 Val Val Leu Lys Arg Asp Ala Asp Ser Tyr Ser Gly Asn Cys Pro Val 50 55 60 Thr Ser Gly Ser Trp Tyr Ser Tyr Tyr Gly Val Thr Phe Thr Asn 65 70 75 80 Pro Ser Asp Leu Asp Ile Asp His Ile Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Ser Lys Arg Gin Asp Phe Ala 100 105 110 Asn Asp Leu Ser Gly Pro Gin Leu Ile Ala Val Ser Ala Ser Thr Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Ser Ser 130 135 140 Gly Ala Ala Cys Gly Tyr Ser Lys Trp Trp Ile Ser Thr Lys Tyr Lys 145 150 155 160 Trp Gly Leu Ser Leu Gin Ser Ser Glu Lys Thr Ala Leu Gin Gly Met 165 170 175 Leu Asn Ser Cys Ser Tyr 180 <210> 14 <211> 182 <212> PRT <213> Bacillus sp-18318 <4 00> 14 Phe Pro Pro Gly Thr Pro Ser Lys Ser Thr Ala Gin Ser Gin Leu Asn 15 10 15 Ser Leu Thr Val Lys Ser Glu Gly Ser With Thr Gly Tyr Ser Arg Asp 20 25 30 Lys Phe Pro His Trp Ile Gly Gin Gly Ser Gly Cys Asp Thr Arg Gin 35 40 45 Leu Val Leu Gin Arg Asp Ala Asp Tyr Tyr Ser Gly Ser Cys Pro Val 50 55 60 Thr Ser Gly Lys Trp Tyr Ser Tyr Tyr Asp Gly Val Thr Phe Tyr Asp 65 70 75 80 Pro Ser Asp Leu Asp Ile Asp His Val Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Ser Thr Gin Lys Arg Lys Asp Phe Ala 100 105 110 Asn Asp Leu Ser Gly Pro Gin Leu Ile Ala Val Ser Ala Ser Ser Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Thr Arg Ser 130 135 140 Gly Ala Ala Cys Gly Tyr Ser Lys Trp Trp Ile Ser Thr Lys His Lys 145 150 155 160 Trp Gly Leu Ser Leu Gin Ser Ser Glu Lys Asn Ala Leu Gin Gly Met 165 170 175 Leu Asn Ser Cys Val Tyr 180 <210> 15 <211> 182 <212> PRT <21 3> Bacillus idriensis <4 00> 15 Leu Pro Pro Gly Thr Pro Ser Lys Ser Thr Ala Gin Ser Gin Leu Asn 15 10 15 Ala Leu Thr Val Gin Thr Glu Gly Ser With Thr Gly Tyr Ser Arg Asp 20 25 30 Lys Phe Pro His Trp Ile Ser Gin Gly Asn Gly Cys Asp Thr Arg Gin 35 40 45 Val Val Leu Gin Arg Asp Ala Asp Tyr Tyr Ser Gly Thr Cys Pro Val 50 55 60 Thr Ser Gly Lys Trp Tyr Ser Tyr Tyr Asp Gly Val Thr Leu Tyr Asn 65 70 75 80 Pro Ser Asp Leu Asp Ile Asp His Val Val Ala Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr List Arg Glu Asp Phe Ala 100 105 110 Asn Asp Leu Ser Gly Thr Gin Leu Ile Ala Val Ser Ala Ser Thr Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Ser 130 135 140 Gly Ala Ala Cys Gly Tyr Ala Lys Trp Trp Ile Ser Thr Lys Tyr Lys 145 150 155 160 Trp Asn Leu Asn Leu Gin Ser Ser Glu Lys Thr Ala Leu Gin Ser Met 165 170 175 Leu Asn Ser Cys Ser Tyr 180 <210> 16 <211> 182 <212> PRT <213> Bacillus algicola <400> 16 Phe Pro Pro Gly Thr Pro Ser Lys Ser Glu Ala Gin Ser G in Leu Asn 1 5 10 15 Ser Leu Thr Val Gin Ser Glu Gly Ser With Ser Gly Tyr Ser Ara Asd 20 25 30 List Phe Pro His Trp Ile Gly Gin Gly Asn Gly Cys Asp Thr Arq Gin 35 40 45 Leu Val Leu Gin Arg Asp Ala Asp Tyr Tyr Ser Gly Asp Cys Pro Val 50 55 60 Thr Ser Gly List Trp Tyr Ser Tyr Phe Asp Gly Val Thr Val Tyr Asp 65 70 75 80 Pro Ser Asp Leu Asp Ile Asp His Met Val Pro With Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Ser Thr Gin Lys Arg Glu Asp Phe Ala 100 105 110 Asn Asp Leu Ser Gly Pro His Leu Ile Ala Val Thr Ala Ser Ser Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Lys Pro Thr Arg Tyr 130 135 140 Gly Ala His Cys Gly Tyr Ala Lys Trp Trp Ile Asn Thr Lys Tyr Val 145 150 155 160 Tyr Asp Leu Thr Leu Gin Ser Ser Glu Lys Thr Glu Leu Gin Ser Met 165 170 175 Leu Asn Thr Cys Ser Tyr 180 <210> 17 <211> 182 <212> PRT <213> Enviromental sample J <4OO> 17 Leu Pro Pro Asn Ile Pro Ser Lys Ala Asp Ala Leu Thr Lys Leu Asn 15 10 15 Ala Leu Thr Val Gin Thr Glu Gly Pro With Thr Gly Tyr Ser Arg Asp 20 25 30 Leu Phe Pro His Trp Ser Ser Gin Gly Asn Gly Cys Asn Thr Arg His 35 40 45 Val Val Leu Lys Arg Asp Ala Asp Ser Val Val Asp Thr Cys Pro Val 50 55 60 Thr Thr Gly Arg Trp Tyr Ser Tyr Tyr Asp Gly Leu Val Phe Thr Ser 65 70 75 80 Ala Ser Asp Ile Asp Ile Asp His Val Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Ser Thr Lys Arg Gin Ser Phe Ala 100 105 110 Asn Asp Leu Asn Gly Pro Gin Leu Ile Ala Val Ser Ala Thr Ser Asn 115 120 125 Arg Ser List Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Ala 130 135 140 Gly Ala Arg Cys Ala Tyr Ala Lys With Trp Val Glu Thr Lys Ser Arg 145 150 155 160 Trp Gly Leu Thr Leu Gin Ser Ser Glu Lys Ala Ala Leu Gin Thr Ala 165 170 175 Ile Asn Ala Cys Ser Tyr 180 <210> 18 <211> 182 <212> PRT <213> Bacillus vietnamensis <4 OO> 18 Phe Pro Pro Gly Thr Pro Ser Lys Ser Thr Ala Gin Ser Gin Leu Asn 15 10 15 Ala Leu Thr Val Lys Ser Glu Ser Ser With Thr Gly Tyr Ser Arg Asp 20 25 30 Lys Phe Pro His Trp Ile Gly Gin Arg Asn Gly Cys Asp Thr Arg Gin 35 40 45 Leu Val Leu Gin Arg Asp Ala Asp Ser Tyr Ser Gly Ser Cys Pro Val 50 55 60 Thr Ser Gly Ser Trp Tyr Ser Tyr Tyr Asp Gly Val Thr Phe Thr Asp 65 70 75 80 Pro Ser Asp Leu Asp Ile Asp His Val Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Ala Lys Arg Glu Asp Phe Ala 100 105 110 Asn Asp Leu Ser Gly Pro Gin Leu Ile Ala Val Ser Ala Ser Ser Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Ser 130 135 140 Gly Ala Ala Cys Gly Tyr Ser Lys Trp Trp Ile Ser Thr Lys Tyr Lys 145 150 155 160 Trp Gly Leu Ser Leu Gin Ser Ser Glu Lys Thr Ala Leu Gin Gly Met 165 170 175 Leu Asn Ser Cys Ile Tyr 180 <210> 19 <211> 182 <212> PRT <213> Bacillus hwajinpoensis <4 00> 19 Ile Pro Pro Gly Thr Pro Ser Lys Ser Ala Ala Gin Ser Gin Leu Asp 15 10 15 Ser Leu Ala Val Gin Ser Glu Gly Ser With Ser Gly Tyr Ser Arcr Asp 20 25 3o Lys Phe Pro His Trp He Gly Gin Gly Asn Gly Cys Asp Thr Arq Gin 35 40 45 Leu Val Leu Gin Arg Asp Ala Asp Tyr Tyr Ser Gly Asp Cys Pro Val 50 55 60 Thr Ser Gly List Trp Tyr Ser Tyr Phe Asp Gly Val Gin Val Tyr Asp 65 70 75 80 Pro Ser Tyr Leu Asp lie Asp His With Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Ser Thr Gin Lys Arg Glu Asp Phe Ala 100 105 110 Asn Asp Leu Asp Gly Pro His Leu He Ala Val Thr Ala Ser Ser Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Lys Pro Thr Arg Tyr 130 135 140 Ser Ala His Cys Gly Tyr Ala Lys Trp Trp He Asn Thr Lys Tyr Val 145 150 155 160 Tyr Asp Leu Asn Leu Gin Ser Ser Glu Lys Ser Ala Leu Gin Ser Met 165 170 175 Leu Asn Thr Cys Ser Tyr 180 <210> 20 <211 > 182 <212> PRT <213> Paenibacillus mucilaginosus <4 00> 20 Leu Pro Pro Gly Thr Pro Ser Lys Ser Thr Ala Gin Ser Gin Leu Asn 15 10 15 Ser Leu Thr Val Lys Ser Glu Ser Thr With Thr Gly Tyr Ser Arg Asp 20 25 30 Lys Phe Pro His Trp Thr Ser Gin Gly Gly Gly Cys Asp Thr Arg Gin 35 40 45 Val Val Leu Lys Arg Asp Ala Asp Tyr Tyr Ser Gly Ser Cys Pro Val 50 55 60 Thr Ser Gly Lys Trp Tyr Ser Tyr Tyr Asp Gly lie Thr Val Tyr Ser 65 70 75 80 Pro Ser Glu lie Asp lie Asp His lie Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Glu Lys Arg Gin Asn Phe Ala 100 105 110 Asn Asp Leu Gly Gly Pro Gin Leu lie Ala Val Thr Ala Ser Ser Asn 115 120 125 Arg Ala Lys Gly Asp Gin Asp Pro Ser Thr Trp Lys Pro Thr Arg Ser 130 135 140 Gly Ala His Cys Ala Tyr Ala Lys Trp Trp lie Asn Thr Lys Tyr Arg 145 150 155 160 Trp Gly Leu His Leu Gin Ser Ser Glu Lys Thr Ala Leu Gin Ser Met 165 170 175 Leu Asn Thr Cys Ser Tyr 180 <210> 21 <211> 182 <212> PRT <213> Bacillus indicus <400> 21 Thr Pro Pro Gly Thr Pro Ser Lys Ser Thr Ala Gin Thr Gin Leu Asn 15 10 15 Ala Leu Thr Val Lys Thr Glu Gly Ser With Thr Gly Tyr Ser Arg Asp 20 25 30 Leu Phe Pro His Trp lie Ser Gin Gly Ser Gly Cys Asp Thr Arg Gin 35 40 45 Val Val Leu Lys Arg Asp Ala Asp Tyr Tyr Ser Gly Ser Cys Pro Val 50 55 60 Thr Ser Gly Lys Trp Tyr Ser Tyr Tyr Asp Gly Val Thr Phe Tyr Asp 65 70 75 80 Pro Ser Asp Leu Asp lie Asp His lie Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg S is Gly Ala Ser Ser Trp Thr Thr Ser Lys Arg Gin Asp Phe Ala 100 105 110 Asn Asp Leu Ser Gly Pro Gin Leu lie Ala Val Ser Ala Ser Thr Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Ala 130 135 140 Gly Ala Ala Cys Gly Tyr Ser Lys Trp Trp lie Ser Thr Lys Tyr Lys 145 150 155 160 Trp Gly Leu Ser Leu Gin Ser Ser Glu Lys Thr Ala Leu Gin Gly Met 165 170 175 Leu Asn Ser Cys Ser Tyr 180 <210> 22 <211> 182 <212> PRT <213> Bacillus marisflavi <400> 22 Thr Pro Pro Thr Thr Ser Lys Ala Thr Ser Gin Ser Gin Leu Asn 15 10 15 Gly Leu Thr Val Lys Thr Glu Gly Ala With Thr Gly Tyr Ser Arg Asp 20 25 30 Lys Phe Pro His Trp Ser Ser Gin Gly Gly Gly Cys Asp Thr Arg Gin 35 40 45 Val Val Leu Lys Arg Asp Ala Asp Ser Tyr Ser Gly Asn Cys Pro Val 50 55 60 Thr Ser Gly Ser Trp Tyr Ser Tyr Tyr Asp Gly Val Lys Phe Thr Asn 65 70 75 80 Pro Ser Asp Leu Asp Ile Asp His Ile Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Ala Gin Arg Glu Ala Phe Ala 100 105 110 Asn Asp Leu Ser Gly Ser Gin Leu Ile Ala Val Ser Ala Ser Ser Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Ala 130 135 140 Gly Ala Lys Cys Gly Tyr Ala Lys Trp Trp Ile Ser Thr Lys Ser List 145 150 155 160 Trp Asn Leu Ser Leu Gin Ser Ser Glu List Thr Ala Leu Gin Gly Met 165 170 175 Leu Asn Ser Cys Val Tyr 180 <210> 23 <211> 184 <212> PRT <213> Bacillus luciferensis <4 00> 23 Ala Ser Leu Pro Pro Gly Ile Pro Ser Leu Ser Thr Ala Gin Ser Gin 15 10 15 Leu Asn Ser Leu Thr Val Lys Ser Glu Gly Ser Leu Thr Gly Tyr Ser 20 25 30 Arg Asp Val Phe Pro His Trp Ile Ser Gin Gly Ser Gly Cys Asp Thr 35 40 45 Arg Gin Val Val Leu Lys Arg Asp Ala Asp Tyr Tyr Ser Gly Asn Cys 50 55 60 Pro Val Thr Ser Gly Lys Trp Tyr Ser Tyr Tyr Asp Gly Val Thr Val 65 70 75 80 Tyr Ser Pro Ser Glu Ile Asp Ile Asp His Val Val Pro Alu Glu 85 90 95 Ala Trp Arg Ser Gly Ala Ser Ser Trp Thr Thr Glu Lys Arg Gin Asn 100 105 110 Phe Ala Asn Asp Leu Asn Gly Pro Gin Leu Ile Ala Val Thr Ala Ser 115 120 125 Ser Asn Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Thr Gin Pro Thr 130 135 140 Arg Thr Gly Ala Arg Cys Ala Tyr Ala Lys With Trp Ile Asn Thr Lys 145 150 155 160 Tyr Arg Trp Gly Leu His Leu Gin Ser Ser Glu Lys Ser Ala Leu Gin 165 170 175 Ser With Leu Asn Thr Cys Ser Tyr 180 <210> 24 <211> 182 <212> PRT <213> Bacillus marisflavi <4 00> 24 Thr Pro Pro Val Thr Pro Ser Lys Glu Thr Ser Gin Ser Gin Leu Asn 15 10 15 Gly Leu Thr Val Lys Thr Glu Gly Ala With Thr Gly Tyr Ser Arg Asp 20 25 30 Lys Phe Pro His Trp Ser Ser Gin Gly Gly Gly Cys Asp Thr Arg Gin 35 40 45 Val Val Leu Lys Arg Asp Ala Asp Ser Tyr Ser Gly Asn Cys Pro Val 50 55 60 Thr Ser Gly Ser Trp Tyr Ser Tyr Tyr Asp Gly Val Lys Phe Thr His 65 70 75 80 Pro Ser Asp Leu Asp Ile Asp His Ile Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Ala Gin Arg Glu Ala Phe Ala 100 105 110 Asn Asp Leu Ser Gly Ser Gin Leu Ile Ala Val Ser Ala Ser Ser Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Ala 130 135 140 Gly Ala List C ice Gly Tyr Ala Lys Trp Trp Ile Ser Thr Lys Ser Lys 145 150 155 160 Trp Asn Leu Ser Leu Gin Ser Ser Glu Lys Thr Ala Leu Gin Gly Met 165 170 175 Leu Asn Ser Cys Val Tyr 180 <210> 25 <211> 182 <212> PRT <213> Bacillus sp. SA2-6 <4 00> 25 Leu Pro Ser Gly Ile Pro Ser Lys Ser Thr Ala Gin Ser Gin Leu Asn 15 10 15 Ser Leu Thr Val Lys Ser Glu Gly Ser Met Thr Gly Tyr Ser Arg Asp 20 25 30 Lys Phe Pro His Trp Ile Ser Gin Gly Gly Gly Cys Asp Thr Arg Gin 35 40 45 Val Val Leu Lys Arg Asp Ala Asp Tyr Tyr Ser Gly Asn Cys Pro Val 50 55 60 Thr Ser Gly Lys Trp Tyr Ser Tyr Tyr Asp Gly Ile Ser Val Tyr Ser 65 70 75 80 Pro Ser Glu Ile Asp Ile Asp His Val Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Thr Lys Arg Gin Asn Phe Ala 100 105 110 Asn Asp Leu Asn Gly Pro Gin Leu Ile Ala Val Thr Ala Ser Val Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Tyr 130 135 140 Gly Ala Arg Cys Ala Tyr Ala Lys Met Trp Ile Asn Thr Lys Tyr Arg 145 150 155 160 Trp Asp Leu Asn Leu Gin Ser Ser Glu Lys Ser Ser Leu Gin Ser Met 165 170 175 Leu Asp Thr Cys Ser Tyr 180 <210> 26 <211> 191 <212> PRT <213> Pyrenochaetopsis sp.SA2-6 <4 00> 25 Leu Pro Ser Gly Ile Pro Ser Lys Ser Thr Ala Gin Ser Gin Leu Asn 15 10 15 Ser Leu Thr Val Lys Ser Glu Gly Ser With Thr Gly Tyr Ser Arg Asp 20 25 30 Lys Phe Pro His Trp Ile Ser Gin Gly Gly Gly Cys Asp Thr Arg Gin 35 40 45 Val Val Leu Lys Arg Asp Ala Asp Tyr Tyr Ser Gly Asn Cys Pro Val 50 55 60 Thr Ser Gly Light Trp Tyr Ser Tyr Tyr Asp Gly Ile Ser Val Tyr Ser 65 70 75 80 Pro Ser Glu Ile Asp Ile Asp His Val Val Pro Leu Ala Glu Ala Trp 85 90 95 Arg Ser Gly Ala Ser Ser Trp Thr Thr Thr Arg Arg Gin Asn Phe Ala 100 105 110 Asn Asp Leu Asn Gly Pro Gin Leu Ile Ala Val Thr Ala Ser Val Asn 115 120 125 Arg Ser Lys Gly Asp Gin Asp Pro Ser Thr Trp Gin Pro Pro Arg Tyr 130 135 140 Gly Ala Arg Cys Ala Tyr Ala Lys With Trp Ile Asn Thr Lys Tyr Arg 145 150 155 160 Trp Asp Leu Asn Leu Gin Ser Ser Glu Lys Ser Ser Leu Gin Ser Met 165 170 175 Leu Asp Thr Cys Ser Tyr 180 <210> 26 <211> 191 <212> PRT <213> Pyrenochaetopsis sp. <4 00> 26 Leu Pro Ser Pro Leu Leu Ile Ala Arg Ser Pro Pro Asn Ile Pro Ser 15 10 15 Ala Thr Thr Ala Lys Thr Gin Leu Ala Gly Leu Thr Val Ala Pro Gin 20 25 30 Gly Pro Gin Thr Gly Tyr Ser Arg Asp Leu Phe Pro His Trp Ile Thr 35 40 45 Gin Ser Gly Thr Cys Asn Thr Arg Glu Val Val Leu Lys Arg Asp Gly 50 55 60 Thr Asn Val Val Thr Asn Ser Ala Cys Ala Ser Thr Ser Gly Ser Trp 65 70 75 80 Leu Ser Pro Tyr Asp Gly Lys Thr Trp Asp Ser Ala Ser Asp Ile Gin 85 90 95 Ile Asp His Leu Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala Ala 100 105 110 Ala Trp Thr Thr Ala Gin Arg Gin Ala Phe Ala Asn Asp Leu Thr His 115 120 125 Pro Gin Leu Val Ala Val Thr Gly Ser Val Asn Glu Ser Lys Gly Asp 130 135 140 Asp Gly Pro Glu Asp Trp Lys Pro Pro Leu Ala Ser Tyr Tyr Cys Thr 145 150 155 160 Tyr Ala Ser Met Trp Thr Ala Val Lys Ser Asn Tyr Lys Leu Thr Ile 165 170 175 Thr Ser Ala Glu Lys Ser Ala Leu Thr Ser Met Leu Ala Thr Cys 180 185 190 <210> 27 <211> 190 <212> PRT <213> Vibrissea flavovirens <4 00> 27 Thr Pro Leu Pro Ile Ile Ala Arg Thr Pro Pro Asn Ile Pro Thr Thr 15 10 15 Ala Thr Ala Lys Ser Gin Leu Ala Ala Leu Thr Val Ala Ala Ala Gly 20 25 30 Pro Gin Thr Gly Tyr Ser Arg Asp Leu Phe Pro Thr Trp lie Thr lie 35 40 45 Ser Gly Thr Cys Asn Thr Arg Glu Thr Val Leu Lys Arg Asp Gly Thr 50 55 60 Asn Val Val Val Asp Ser Ala Cys Val Ala Thr Ser Gly Ser Trp Tyr 65 70 75 80 Ser Pro Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val Asp He 85 90 95 Asp His Met Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala Ser Ala 100 105 110 Trp Thr Thr Ala Gin Arg Gin Thr Phe Ala Asn Asp Leu Thr Asn Pro 115 120 125 Gin Leu Leu Ala Val Thr Asp Asn Val Asn Gin Ala Lys Gly Asp Ser 130 135 140 Gly Pro Glu Asp Trp Lys Pro Ser Leu Thr Ser Tyr Trp Cys Thr Tyr 145 150 155 160 Ala Lys Met Trp Val Lys Val Lys Thr Val Tyr Asp Leu Thr lie Thr 165 170 175 Ser Ala Glu Lys Thr Ala Leu Thr Thr Met Leu Asn Thr Cys 180 185 190 <210> 28 <211> 192 <212> PRT <213> Setosphaeria rostrata <4 00> 28 Ala Pro Thr Ser Ser Pro Leu Val Ala Arg Ala Pro Pro Asn Val Pro 1 5 10 15 Ser Lys Ala Glu Ala Thr Ser Gin Leu Ala Gly Leu Thr Val Ala Pro 20 25 30 Gin Gly Pro Gin Thr Gly Tyr Ser Arg Asp Leu Phe Pro His Trp lie 35 40 45 Thr Gin Ser Gly Thr Cys Asn Thr Arg Glu Thr Val Leu Lys Arg Asp 50 55 60 Gly Thr Asn Val Val Thr Asn Ser Ala Cys Ala Ser Thr Ser Gly Ser 65 70 75 80 Trp Phe Ser Pro Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val 85 90 95 Asp lie Asp His Met Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala 100 105 110 Ala Ser Trp Thr Thr Ala Arg Arg Gin Ala Phe Ala Asn Asp Leu Thr 115 120 125 Asn Pro Gin Leu Leu Ala Val Thr Asp Asn Val Asn Gin Ala Lys Gly 130 135 140 Asp Lys Gly Pro Glu Asp Trp Lys Pro Pro Leu Thr Ser Tyr Tyr Cys 145 150 155 160 Thr Tyr Ser Lys Met Trp He Lys Val Lys Ser Val Trp Gly Leu Thr 165 170 175 lie Thr Ser Ala Glu Lys Ser Ala Leu Thr Ser Met Leu Ala Thr Cys 180 185 190 <210> 29 <211> 192 <212> PRT <213> Endophragmiella valdina <400> 29 Ala Pro Val Pro Gly His Leu Met Pro Arg Ala Pro Pro Asn Val Pro 15 10 15 Thr Thr Ala Ala Ala Lys Thr Ala Leu Ala Gly Leu Thr Val Gin Ala 20 25 30 Gin Gly Ser Gin Thr Gly Tyr Ser Arg Asp Leu Phe Pro His Trp He 35 40 45 Thr Gin Ser Gly Thr Cys Asn Thr Arg Glu Val Val Leu Lys Arg Asp 50 55 60 Gly Thr Asn Val Val Thr Asp Ser Ala Cys Ala Ala Thr Ser Gly Thr 65 70 75 80 Trp Val Ser Pro Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val 85 90 95 Asp Ile Asp His Met Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala 100 105 110 Ala Ser Trp Thr Thr Ala Gin Arg Gin Ala Phe Ala Asn Asp Leu Thr 115 120 125 Asn Pro Gin Leu Leu Ala Val Thr Asp Asn Val Asn Gin Ser Lys Gly 130 135 140 Asp Lys Gly Pro Glu Asp Trp Lys Pro Pro Leu Thr Ser Tyr Tyr Cys 145 150 155 160 Thr Tyr Ala Lys Met Trp Val Lys Val Lys Ser Val Tyr Ser Leu Thr 165 170 175 Ile Thr Ser Ala Glu Lys Thr Ala Leu Thr Ser Met Leu Asn Thr Cys 180 185 190 <210> 30 <211> 190 <212> PRT <213> Corynespora cassiicola <4 00> 30 Leu Pro Ala Pro Leu Val Pro Arg Ala Pro Pro Gly Ile Pro Thr Thr 15 10 15 Ser Ala Ala Arg Ser Gin Leu Ala Gly Leu Thr Val Ala Ala Gin Gly 20 25 30 Pro Gin Thr Gly Tyr Ser Arg Asp Leu Phe Pro His Trp Ile Thr Gin 35 40 45 Ser Gly Ser Cys Asn Thr Arg Glu Val Val Leu Ala Arg Asp Gly Thr 50 55 60 Gly Val Val Gin Asp Ser Ser Cys Ala Ala Thr Ser Gly Thr Trp Arg 65 70 75 80 Ser Pro Phe Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val Asp Ile 85 90 95 Asp His Met Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala Ala Ser 100 105 110 Trp Thr Thr Ser Arg Arg Gin Ala Phe Ala Asn Asp Leu Thr Asn Pro 115 120 125 Gin Leu Ile Ala Val Thr Asp Asn Val Asn Gin Ser Lys Gly Asp Lys 130 135 140 Gly Pro Glu Asp Trp Lys Pro Pro Leu Thr Ser Tyr Tyr Cys Thr Tyr 145 150 155 160 Ala Lys Met Trp Val Arg Val Lys Ser Val Tyr Ser Leu Thr Ile Thr 165 170 175 Ser Ala Glu Lys Ser Ala Leu Thr Ser Met Leu Asp Thr Cys 180 185 190 <210> 31 <211> 192 <212> PRT <213> Paraphoma sp.<4 00> 26 Leu Pro Ser Pro Leu Leu Ile Ala Arg Ser Pro Pro Asn Ile Pro Ser 15 10 15 Ala Thr Thr Ala Lys Thr Gin Leu Ala Gly Leu Thr Val Ala Pro Gin 20 25 30 Gly Pro Gin Thr Gly Tyr Ser Arg Asp Leu Phe Pro His Trp Ile Thr 35 40 45 Gin Ser Gly Thr Cys Asn Thr Arg Glu Val Val Leu Lys Arg Asp Gly 50 55 60 Thr Asn Val Val Thr Asn Ser Ala Cys Ala Ser Thr Ser Gly Ser Trp 65 70 75 80 Leu Ser Pro Tyr Asp Gly Lys Thr Trp Asp Ser Ala Ser Asp Ile Gin 85 90 95 Ile Asp His Leu Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala Ala 100 105 110 Ala Trp Thr Thr Ala Gin Arg Gin Ala Phe Ala Asn Asp Leu Thr His 115 120 125 Pro Gin Leu Val Ala Val Thr Gly Ser Val Asn Glu Ser Lys Gly Asp 130 135 140 Asp Gly Pro Glu Asp Trp Lys Pro Pro Leu Ala Ser Tyr Tyr Cys Thr 145 150 155 160 Tyr Ala Ser With Trp Thr Ala Val Lys Ser Asn Tyr Lys Leu Thr Ile 165 170 175 Thr Ser Ala Glu Lys Ser Ala Leu Thr Ser With Leu Ala Thr Cys 180 185 190 <210> 27 <211> 190 <212> PRT <213> Vibrissea flavovirence <4 00> 27 Thr Pro Leu Pro Ile Ile Al a Arg Thr Pro Pro Asn Ile Pro Thr Thr 15 10 15 Ala Thr Ala Lys Ser Gin Leu Ala Ala Leu Thr Val Ala Ala Ala Gly 20 25 30 Pro Gin Thr Gly Tyr Ser Arg Asp Leu Phe Pro Thr Trp lie Thr lie 35 40 45 Ser Gly Thr Cys Asn Thr Arg Glu Thr Val Leu Lys Arg Asp Gly Thr 50 55 60 Asn Val Val Val Asp Ser Ala Cys Val Ala Thr Ser Gly Ser Trp Tyr 65 70 75 80 Ser Pro Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val Asp He 85 90 95 Asp His With Val Pro Leu Ser Asn Ala Trp List Ser Gly Ala Ser Ala 100 105 110 Trp Thr Thr Ala Gin Arg Gin Thr Phe Ala Asn Asp Leu Thr Asn Pro 115 120 125 Gin Leu Leu Ala Val Thr Asp Asn Val Asn Gin Ala Lys Gly Asp Ser 130 135 140 Gly Pro Glu Asp Trp Lys Pro Ser Leu Thr Ser Tyr Trp Cys Thr Tyr 145 150 155 160 Ala Lys With Trp Val Lys Val Lys Thr Val Tyr Asp Leu Thr lie Thr 165 170 175 Ser Ala Glu Lys Thr Ala Leu Thr Thr With Leu Asn Thr Cys 180 185 190 <210> 28 <211> 192 <212> PRT <213> Setosphaeria rostrata <400> 28 Ala Pro Thr Ser Ser Pro Leu Val Ala Arg Ala Pro Pro Asn Val Pro 1 5 10 15 Ser Lys Ala Glu Ala Thr Ser Gin Leu Ala Gly Leu Thr Val Ala Pro 20 25 30 Gin Gly Pro Gin Thr Gly Tyr Ser Arg Asp Leu Phe Pro His Trp lie 35 40 45 Thr Gin Ser Gly Thr Cys Asn Thr Arg Glu Thr Val Leu Lys Arg Asp 50 55 60 Gly Thr Asn Val Val Thr Asn Ser Ala Cys Ala Ser Thr Ser Gly Ser 65 70 75 80 Trp Phe Ser Pro Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val 85 90 95 Asp lie Asp His With Val Pro Leu Ser Asn Ala Trp List Ser Gly Ala 100 105 110 Ala Ser Trp Thr Thr Ala Arg Arg Gin Ala Phe Ala Asn Asp Leu Thr 115 120 125 Asn Pro Gin Leu Leu Ala Val Thr Asp Asn Val Asn Gin Ala Lys Gly 130 135 140 Asp Lys Gly Pro Glu Asp Trp Lys Pro Pro Leu Thr Ser Tyr Tyr Cys 145 150 155 160 Thr Tyr Ser Lys With Trp He Lys Val Lys Ser Val Trp Gly Leu Thr 165 170 175 lie Thr Ser Ala Glu Lys Ser Ala Leu Thr Ser With Leu Ala Thr Cys 180 185 190 <210> 29 <211> 192 <212> PRT <213> Endophragmiella valdina <400> 29 Ala Pro Val Pro Gly His Leu With Pro Arg Ala Pro Pro Asn Val Pro 15 10 15 Thr Thr Ala Ala Ala Lys T Mr Ala Leu Ala Gly Leu Thr Val Gin Ala 20 25 30 Gin Gly Ser Gin Thr Gly Tyr Ser Arg Asp Leu Phe Pro His Trp He 35 40 45 Thr Gin Ser Gly Thr Cys Asn Thr Arg Glu Val Val Leu Arg Arg Asp 50 55 60 Gly Thr Asn Val Val Thr Asp Ser Ala Cys Ala Ala Thr Ser Gly Thr 65 70 75 80 Trp Val Ser Pro Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val 85 90 95 Asp Ile Asp His With Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala 100 105 110 Ala Ser Trp Thr Thr Ala Gin Arg Gin Ala Phe Ala Asn Asp Leu Thr 115 120 125 Asn Pro Gin Leu Leu Ala Val Thr Asp Asn Val Asn Gin Ser Lys Gly 130 135 140 Asp Lys Gly Pro Glu Asp Trp List Pro Pro Leu Thr Ser Tyr Tyr Cys 145 150 155 160 Thr Tyr Ala Lys With Trp Val Lys Val Lys Ser Val Tyr Ser Leu Thr 165 170 175 Ile Thr Ser Ala Glu Lys Thr Ala Leu Thr Ser With Leu Asn Thr Cys 180 185 190 <210> 30 <211> 190 <212> PRT <213> Corynespora cassiicola <4 00> 30 Leu Pro Ala Pro Leu Val Pro Arg Ala Pro Pro Gly Ile Pro Thr Thr 15 10 15 Ser Ala Ala Arg Ser Gin Leu Ala Gly Leu Thr Val Ala Ala G in Gly 20 25 30 Pro Gin Thr Gly Tyr Ser Arg Asp Leu Phe Pro His Trp Ile Thr Gin 35 40 45 Ser Gly Ser Cys Asn Thr Arg Glu Val Val Leu Ala Arg Asp Gly Thr 50 55 60 Gly Val Val Gin Asp Ser Ser Cys Ala Ala Thr Ser Gly Thr Trp Arg 65 70 75 80 Ser Pro Phe Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val Asp Ile 85 90 95 Asp His With Val Pro Leu Ser Asn Ala Trp List Ser Gly Ala Ala Ser 100 105 110 Trp Thr Thr Ser Arg Arg Gin Ala Phe Ala Asn Asp Leu Thr Asn Pro 115 120 125 Gin Leu Ile Ala Val Thr Asp Asn Val Asn Gin Ser List Gly Asp List 130 135 140 Gly Pro Glu Asp Trp List Pro Pro Leu Thr Ser Tyr Tyr Cys Thr Tyr 145 150 155 160 Ala Lys With Trp Val Arg Val Lys Ser Val Tyr Ser Leu Thr Ile Thr 165 170 175 Ser Ala Glu Lys Ser Ala Leu Thr Ser With Leu Asp Thr Cys 180 185 190 <210> 31 < 211> 192 <212> PRT <213> Paraphoma sp. XZ1965 <4 00> 31 Ala Pro Ala Pro Val His Leu Val Ala Arg Ala Pro Pro Asn Val Pro 1 5 10 15 Thr Ala Ala Gin Ala Gin Thr Gin Leu Ala Gly Leu Thr Val Ala Ala 20 25 30 Gin Gly Pro Gin Thr Gly Tyr Ser Arg Asp Leu Phe Pro His Trp Ile 35 40 45 Thr Gin Ser Gly Ala Cys Asn Thr Arg Glu Thr Val Leu Lys Arg Asp 50 55 60 Gly Thr Gly Val Val Gin Asp Ser Ala Cys Ala Ala Thr Ser Gly Thr 65 70 75 80 Trp Lys Ser Pro Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val 85 90 95 Asp Ile Asp His Met Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala 100 105 110 Ala Ser Trp Thr Thr Ala Arg Arg Gin Ala Phe Ala Asn Asp Leu Thr 115 120 125 Asn Pro Gin Leu Leu Ala Val Thr Asp Asn Val Asn Gin Ala Lys Gly 130 135 140 Asp Lys Gly Pro Glu Asp Trp Lys Pro Pro Leu Thr Ser Tyr Tyr Cys 145 150 155 160 Ile Tyr Ala Arg Met Trp Ile Lys Val Lys Ser Val Tyr Ser Leu Thr 165 170 175 Ile Thr Ser Ala Glu Lys Ser Ala Leu Thr Ser Met Leu Gly Thr Cys 180 185 190 <210> 32 <211> 186 <212> PRT <213> Monilinia fructicola <4 00> 32 Thr Pro Val Pro Ala Pro Thr Gly Ile Pro Ser Thr Ser Val Ala Asn 15 10 15 Thr Gin Leu Ala Ala Leu Thr Val Ala Ala Ala Gly Ser Gin Asp Gly 20 25 30 Tyr Ser Arg Asp Leu Phe Pro His Trp Ile Thr Ile Ser Gly Ala Cys 35 40 45 Asn Thr Arg Glu Thr Val Leu Lys Arg Asp Gly Thr Asn Val Val Val 50 55 60 Asn Ser Ala Cys Ala Ala Thr Ser Gly Thr Trp Val Ser Pro Tyr Asp 65 70 75 80 Gly Ala Thr Trp Thr Ala Ala Ser Asp Val Asp Ile Asp His Leu Val 85 90 95 Pro Leu Ser Asn Ala Trp Lys Ala Gly Ala Ser Ser Trp Thr Thr Ala 100 105 110 Gin Arg Gin Ala Phe Ala Asn Asp Leu Val Asn Pro Gin Leu Leu Ala 115 120 125 Val Thr Asp Ser Val Asn Gin Gly Lys Ser Asp Ser Gly Pro Glu Ala 130 135 140 Trp Lys Pro Ser Leu Lys Ser Tyr Trp Cys Thr Tyr Ala Lys Met Trp 145 150 155 160 Ile Lys Val Lys Tyr Val Tyr Asp Leu Thr Ile Thr Ser Ala Glu Lys 165 170 175 Ser Ala Leu Val Thr Met Met Asp Thr Cys 180 185 <210> 33 <211> 190 <212> PRT <213> Curvularia lunata <400> 33 Ala Pro Ala Pro Leu Ser Ala Arg Ala Pro Pro Asn Ile Pro Ser Lys 15 10 15 Ala Asp Ala Thr Ser Gin Leu Ala Gly Leu Thr Val Ala Ala Gin Gly 20 25 30 Pro Gin Thr Gly Tyr Ser Arg Asp Leu Phe Pro His Trp Ile Thr Gin 35 40 45 Ser Gly Thr Cys Asn Thr Arg Glu Thr Val Leu Lys Arg Asp Gly Thr 50 55 60 Asn Val Val Thr Ser Ser Ser Cys Ala Ala Thr Ser Gly Thr Trp Phe 65 70 75 80 Ser Pro Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val Asp Ile 85 90 95 Asp His Val Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala Ala Ser 100 105 110 Trp Thr Thr Ala Arg Arg Gin Ala Phe Ala Asn Asp Leu Thr Asn Pro 115 120 125 Gin Leu Ile Ala Val Thr Asp Ser Val Asn Gin Ala Lys Gly Asp Lys 130 135 140 Gly Pro Glu Asp Trp Lys Pro Pro Leu Ser Ser Tyr Tyr Cys Thr Tyr 145 150 155 160 Ser Lys Met Trp Ile Lys Val Lys Ser Val Tyr Gly Leu Thr Val Thr 165 170 175 Ser Ala Glu Lys Ser Ala Leu Ser Ser Met Leu Ala Thr Cys 180 185 190 <210> 34 <211> 191 <212> PRT <213> Penicillium reticulisporum <4 00> 34 Leu Pro Ala Pro Glu Ala Leu Pro Ala Pro Pro Gly Val Pro Ser Ala 15 10 15 Ser Thr Ala Gin Ser Glu Leu Ala Ala Leu Thr Val Ala Ala Gin Gly 20 25 30 Ser Gin Asp Gly Tyr Ser Arg Ser Lys Phe Pro His Trp Ile Thr Gin 35 40 45 Ser Gly Ser Cys Asp Thr Arg Asp Val Val Leu Lys Arg Asp Gly Thr 50 55 60 Asn Val Val Gin Ser Ala Ser Gly Cys Thr Ile Thr Ser Gly Lys Trp 65 70 75 80 Val Ser Pro Tyr Asp Gly Ala Thr Trp Thr Ala Ser Ser Asp Val Asp 85 90 95 Ile Asp His Leu Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala Ser 100 105 110 Gly Trp Thr Thr Ala Ala Arg Gin Ala Phe Ala Asn Asp Leu Thr Asn 115 120 125 Pro Gin Leu Leu Val Val Thr Asp Asn Val Asn Glu Ser Lys Gly Asp 130 135 140 Lys Gly Pro Glu Glu Trp Lys Pro Pro Leu Thr Ser Tyr Tyr Cys Thr 145 150 155 160 Tyr Ala Glu Met Trp Val Lys Val Lys Ser Val Tyr Lys Leu Thr Ile 165 170 175 Thr Ser Ala Glu Lys Ser Ala Leu Thr Ser Met Leu Ser Thr Cys 180 185 190 <210> 35 <211> 191 <212> PRT <213> Penicillium quercetorum <4 00> 35 Leu Pro Ala Pro Glu Pro Ala Pro Ser Pro Pro Gly Ile Pro Ser Ala 1 5 10 15 Ser Thr Ala Arg Ser Glu Leu Ala Ser Leu Thr Val Ala Pro Gin Gly 20 25 30 Ser Gin Asp Gly Tyr Ser Arg Ala Lys Phe Pro His Trp Ile Lys Gin 35 40 45 Ser Gly Ser Cys Asp Thr Arg Asp Val Val Leu Glu Arg Asp Gly Thr 50 55 60 Asn Val Val Gin Ser Ser Thr Gly Cys Thr Ile Thr Gly Gly Thr Trp 65 70 75 80 Val Ser Pro Tyr Asp Gly Ala Thr Trp Thr Ala Ser Ser Asp Val Asp 85 90 95 Ile Asp His Leu Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala Ser 100 105 110 Ala Trp Thr Thr Ala Gin Arg Gin Ala Phe Ala Asn Asp Leu Thr Asn 115 120 125 Pro Gin Leu Val Ala Val Thr Asp Asn Val Asn Glu Ala Lys Gly Asp 130 135 140 Lys Gly Pro Glu Glu Trp Lys Pro Pro Leu Thr Ser Tyr Tyr Cys Thr 145 150 155 160 Tyr Ala Glu Met Trp Val Lys Val Lys Ser Val Tyr Lys Leu Thr Ile 165 170 175 Thr Ser Ala Glu Lys Ser Ala Leu Ser Ser Met Leu Asn Thr Cys 180 185 190 <210> 36 <211> 192 <212> PRT <213> Setophaeosphaeria sp.XZ1965 <4 00> 31 Ala Pro Ala Pro Val His Leu Val Ala Arg Ala Pro Pro Asn Val Pro 1 5 10 15 Thr Ala Al Gin Ala Gin Thr Gin Leu Ala Gly Leu Thr Val Ala Ala 20 25 30 Gin Gly Pro Gin Thr Gly Tyr Ser Arg Asp Leu Phe Pro His Trp Ile 35 40 45 Thr Gin Ser Gly Ala Cys Asn Thr Arg Glu Thr Val Leu Lys Arg Asp 50 55 60 Gly Thr Gly Val Val Gin Asp Ser Ala Cys Ala Al Ser Thr Gly Thr 65 70 75 80 Trp List Ser Pro Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val 85 90 95 Asp Ile Asp His Met Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala 100 105 110 Ala Ser Trp Thr Thr Ala Arg Arg Gin Ala Phe Ala Asn Asp Leu Thr 115 120 125 Asn Pro Gin Leu Leu Ala Val Thr Asp Asn Val Asn Gin Ala Lys Gly 130 135 140 Asp Lys Gly Pro Glu Asp Trp Lys Pro Pro Leu Thr Ser Tyr Tyr Cys 145 150 155 160 Ile Tyr Ala Arg With Trp Ile List Val Lys Ser Val Tyr Ser Leu Thr 165 170 175 Ile Thr Ser Ala Glu Lys Ser Ala Leu Thr Ser With Leu Gly Thr Cys 180 185 190 <210> 32 <211> 186 <212> PRT < 213> Monilinia fructicola <4 00> 32 Thr Pro Val Pro Ala Pro Thr Gly Ile Pro Ser Thr Ser Val Ala Asn 15 10 15 Thr Gin Leu Ala Ala Leu Thr Val Ala Ala Gly Ser Gin Asp Gly 20 25 30 Tyr Ser Arg Asp Leu Phe Pro His Trp Ile Thr Ile Ser Gly Ala Cys 35 40 45 Asn Thr Arg Glu Thr Val Leu Lys Arg Asp Gly Thr Asn Val Val Val 50 55 60 Asn Ser Ala Cys Ala Ala Thr Ser Gly Thr Trp Val Ser Pro Tyr Asp 65 70 75 80 Gly Ala Thr Trp Thr Ala Ala Ser Asp Val Asp Ile Asp His Leu Val 85 90 95 Pro Leu Ser Asn Ala Trp Lys Ala Gly Ala Ser Ser Trp Thr Thr Ala 100 105 110 Gin Arg Gin Ala Phe Ala Asn Asp Leu Val Asn Pro Gin Leu Leu Ala 115 120 125 Val Thr Asp Ser Val Asn Gin Gly Ser Ser Asp Ser Gly Pro Glu Ala 130 135 140 Trp Lys Pro Ser Leu Lys Ser Tyr Trp Cys Thr Tyr Ala Lys With Trp 145 150 155 160 Ile Lys Val Lys Tyr Val Tyr Tyr Asp Leu Thr Ile Thr Ser Ala Glu List 165 170 175 Ser Ala Leu Val Thr With Asp Thr Cys 180 185 <210> 33 <211> 190 <212> PRT <213> Curvularia lunata <400> 33 Ala Pro Ala Pro Leu Ser Ala Arg Ala Pro Pro Asn Ile Pro Ser List 15 10 15 Ala Asp Ala Thr Ser Gin Leu Ala Gly Leu Thr Val Ala Ala Gin Gly 20 25 30 Pro Gin Thr Gly Tyr Ser Arg Asp Leu Phe Pro His Trp Ile Thr Gin 35 40 45 Ser Gly Thr Cys Asn Thr Arg Glu Thr Val Leu Lys Arg Asp Gly Thr 50 55 60 Asn Val Val Thr Ser Ser Cys Ala Ala Thr Ser Gly Thr Trp Phe 65 70 75 80 Ser Pro Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val Asp Ile 85 90 95 Asp His Val Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala Ala Ser 100 105 110 Trp Thr Thr Ala Arg Arg Gin Ala Phe Ala Asn Asp Leu Thr Asn Pro 115 120 125 Gin Leu Ile Ala Val Thr Asp Ser Val Asn Gin Ala Lys Gly Asp Lys 130 135 140 Gly Pro Glu Asp Trp Lys Pro Pro Leu Ser Ser Tyr Tyr Cys Thr Tyr 145 150 155 160 Ser Lys Met Trp Ile Lys Val Lys Ser Val Tyr Gly Leu Thr Val Thr 165 170 175 Ser Ala Glu Lys Ser Ala Leu Ser Ser Met Leu Ala Thr Cys 180 185 190 <210> 34 <211> 191 <212> PRT <213> Penicillium reticulisporum <400> 34 Leu Pro Ala Pro Glu Ala Leu Pro Ala Pro Pro Gly Val Pro Ser Ala 15 10 15 Ser Thr Ala Gin Ser Glu Leu Ala Ala Leu Th r Val Ala Ala Gin Gly 20 25 30 Ser Gin Asp Gly Tyr Ser Arg Ser Lys Phe Pro His Trp Ile Thr Gin 35 40 45 Ser Gly Ser Cys Asp Thr Arg Asp Val Val Leu Lys Arg Asp Gly Thr 50 55 60 Asn Val Val Gin Ser Ala Ser Gly Cys Thr Ile Thr Ser Gly Lys Trp 65 70 75 80 Val Ser Pro Tyr Asp Gly Ala Thr Trp Thr Ala Ser Ser Asp Val Asp 85 90 95 Ile Asp His Leu Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala Ser 100 105 110 Gly Trp Thr Thr Ala Ala Arg Gin Ala Phe Ala Asn Asp Leu Thr Asn 115 120 125 Pro Gin Leu Le Val Val Thr Thr Asn Val Asn Glu Ser Lys Gly Asp 130 135 140 Lys Gly Pro Glu Glu Trp Lys Pro Pro Leu Thr Ser Tyr Tyr Cys Thr 145 150 155 160 Tyr Ala Glu Met Trp Val Lys Val Lys Ser Val Tyr Lys Leu Thr Ile 165 170 175 Thr Ser Ala Glu Lys Ser Ala Leu Thr Ser With Leu Ser Thr Cys 180 185 190 <210> 35 <211> 191 <212> PRT <213> Penicillium quercetorum <4 00> 35 Leu Pro Ala Pro Glu Pro Ala Pro Ser Pro Pro Gly Ile Pro Ser Ala 1 5 10 15 Ser Thr Ala Arg Ser Glu Leu Ala Ser Leu Thr Val Ala Pro Gin Gly 20 25 30 Se r Gin Asp Gly Tyr Ser Arg Ala Lys Phe Pro His Trp Ile Lys Gin 35 40 45 Ser Gly Ser Cys Asp Thr Arg Asp Val Val Leu Glu Arg Asp Gly Thr 50 55 60 Asn Val Val Gin Ser Ser Gly Cys Thr Ile Thr Gly Gly Thr Trp 65 70 75 80 Val Ser Pro Tyr Asp Gly Ala Thr Trp Thr Ala Ser Ser Asp Val Asp 85 90 95 Ile Asp His Leu Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala Ser 100 105 110 Ala Trp Thr Thr Al Gin Arg Gin Ala Phe Ala Asn Asp Leu Thr Asn 115 120 125 Pro Gin Leu Val Ala Val Thr Asp Asn Val Asn Glu Ala Lys Gly Asp 130 135 140 Lys Gly Pro Glu Glu Trp Lys Pro Pro Leu Thr Ser Tyr Tyr Cys Thr 145 150 155 160 Tyr Ala Glu Met Trp Val Lys Val Lys Ser Val Tyr Lys Leu Thr Ile 165 170 175 Thr Ser Ala Glu Lys Ser Ala Leu Ser Ser With Leu Asn Thr Cys 180 185 190 <210> 36 <211> 192 < 212> PRT <213> Setophaeosphaeria sp. <4 00> 36 Leu Pro Ala Pro Val Thr Leu Glu Ala Arg Ala Pro Pro Asn Ile Pro 15 10 15 Ser Thr Ala Ser Ala Asn Thr Leu Leu Ala Gly Leu Thr Val Ala Ala 20 25 30 Gin Gly Ser Gin Thr Gly Tyr Ser Arg Asp Leu Phe Pro His Trp Ile 35 40 45 Thr Gin Ser Gly Thr Cys Asn Thr Arg Glu Thr Val Leu Lys Arg Asp 50 55 60 Gly Thr Gly Val Val Thr Asp Ser Ala Cys Ala Ser Thr Ser Gly Ser 65 70 75 80 Trp Tyr Ser Val Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val 85 90 95 Asp Ile Asp His Val Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala 100 105 110 Ala Ser Trp Thr Thr Ala Arg Arg Gin Ser Phe Ala Asn Asp Leu Thr 115 120 125 Asn Pro Gin Leu Ile Ala Val Thr Asp Asn Val Asn Gin Ala Lys Gly 130 135 140 Asp Lys Gly Pro Glu Asp Trp Lys Pro Pro Leu Thr Ser Tyr Tyr Cys 145 150 155 160 Thr Tyr Ala Lys Met Trp Val Lys Val Lys Ser Val Tyr Ser Leu Thr 165 170 175 Ile Thr Ser Ala Glu Lys Thr Ala Leu Thr Ser Met Leu Asn Thr Cys 180 185 190 <210> 37 <211> 192 <212> PRT <213> Alternaria sp.<4 00> 36 Leu Pro Ala Pro Val Thr Leu Glu Ala Arg Ala Pro Pro Asn Ile Pro 15 10 15 Ser Thr Ala Ser Ala Asn Thr Leu Leu Ala Gly Leu Thr Val Ala Ala 20 25 30 Gin Gly Ser Gin Thr Gly Tyr Ser Arg Asp Leu Phe Pro His Trp Ile 35 40 45 Thr Gin Ser Gly Thr Cys Asn Thr Arg Glu Thr Val Leu Lys Arg Asp 50 55 60 Gly Thr Gly Val Val Thr Asp Ser Ala Cys Ala Ser Thr Ser Gly Ser 65 70 75 80 Trp Tyr Ser Val Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val 85 90 95 Asp Ile Asp His Val Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala 100 105 110 Ala Ser Trp Thr Thr Ala Arg Arg Gin Ser Phe Ala Asn Asp Leu Thr 115 120 125 Asn Pro Gin Leu Ile Ala Val Thr Asp Asn Val Asn Gin Ala Lys Gly 130 135 140 Asp Lys Gly Pro Glu Asp Trp Lys Pro Pro Leu Thr Ser Tyr Tyr Cys 145 150 155 160 Thr Tyr Ala List With Trp Val List Val List Ser Val Tyr Ser Leu Thr 165 170 175 Ile Thr Ser Ala Glu List Thr Ala Leu Thr Ser With Leu Asn Thr Cys 180 185 190 <210> 37 <211> 192 <212> PRT <213> Alternaria sp. XZ2545 <4 00> 37 Leu Pro Ala Pro Val Thr Leu Glu Ala Arg Ala Pro Pro Asn Ile Pro 1 5 10 15 Thr Thr Ala Ala Ala Lys Thr Gin Leu Ala Gly Leu Thr Val Ala Ala 20 25 30 Gin Gly Pro Gin Thr Gly Tyr Ser Arg Asp Leu Phe Pro His Trp Ile 35 40 45 Thr Gin Ser Gly Thr Cys Asn Thr Arg Glu Thr Val Leu Lys Arg Asp 50 55 60 Gly Thr Gly Val Val Thr Asp Ser Ala Cys Ala Ser Thr Ser Gly Ser 65 70 75 80 Trp Phe Ser Val Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val 85 90 95 Asp Ile Asp His Val Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala 100 105 110 Ala Ser Trp Thr Thr Ala Arg Arg Gin Ser Phe Ala Asn Asp Leu Thr 115 120 125 Asn Pro Gin Leu Ile Ala Val Thr Asp Asn Val Asn Gin Ala Lys Gly 130 135 140 Asp Lys Gly Pro Glu Asp Trp Lys Pro Pro Leu Thr Ser Tyr Tyr Cys 145 150 155 160 Thr Tyr Ala Lys Met Trp Val Lys Val Lys Ser Val Tyr Ala Leu Thr 165 170 175 Ile Thr Ser Ala Glu Lys Thr Ala Leu Thr Ser Met Leu Asn Thr Cys 180 185 190 <210> 38 <211> 192 <212> PRT <213> Alternaria sp.XZ2545 <4 00> 37 Leu Pro Ala Pro Val Thr Leu Glu Ala Arg Ala Pro Pro Asn Ile Pro 1 5 10 15 Thr Thr Ala Ala Ala Lys Thr Gin Leu Ala Gly Leu Thr Val Ala Ala 20 25 30 Gin Gly Pro Gin Thr Gly Tyr Ser Arg Asp Leu Phe Pro His Trp Ile 35 40 45 Thr Gin Ser Gly Thr Cys Asn Thr Arg Glu Thr Val Leu Lys Arg Asp 50 55 60 Gly Thr Gly Val Val Thr Asp Ser Ala Cys Ala Ser Thr Ser Gly Ser 65 70 75 80 Trp Phe Ser Val Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val 85 90 95 Asp Ile Asp His Val Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala 100 105 110 Ala Ser Trp Thr Thr Ala Arg Arg Gin Ser Phe Ala Asn Asp Leu Thr 115 120 125 Asn Pro Gin Leu Ile Ala Val Thr Asp Asn Val Asn Gin Ala Lys Gly 130 135 140 Asp Lys Gly Pro Glu Asp Trp Lys Pro Pro Leu Thr Ser Tyr Tyr Cys 145 150 155 160 Thr Tyr Ala Lys With Trp Val Lys Val Lys Ser Val Tyr Ala Leu Thr 165 170 175 Ile Thr Ser Ala Glu Lys Thr Ala Leu Thr Ser With Leu Asn Thr Cys 180 185 190 <210> 38 <211> 192 <212> PRT < 213> Alternaria sp. <4 00> 38 Leu Pro Ala Pro Val Thr Leu Glu Ala Arg Ala Pro Pro Asn Ile Pro 1 5 10 15 Thr Thr Ala Ala Ala Lys Thr Gin Leu Ala Gly Leu Thr Val Ala Ala 20 25 30 Gin Gly Pro Gin Thr Gly Tyr Ser Arg Asp Leu Phe Pro His Trp Ile 35 40 45 Thr Gin Ser Gly Ser Cys Asn Thr Arg Glu Val Val Leu Gin Arg Asp 50 55 60 Gly Thr Gly Val Val Thr Asp Ser Ala Cys Ala Ala Thr Ser Gly Ser 65 70 75 80 Trp Tyr Ser Val Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val 85 90 95 Asp Ile Asp His Met Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala 100 105 110 Ala Ser Trp Thr Thr Ala Arg Arg Gin Ala Phe Ala Asn Asp Leu Thr 115 120 125 Asn Pro Gin Leu Leu Ala Val Thr Asp Asn Val Asn Gin Ala Lys Gly 130 135 140 Asp Lys Gly Pro Glu Asp Trp Lys Pro Pro Leu Thr Ser Tyr Tyr Cys 145 150 155 160 Thr Tyr Ala Lys Met Trp Val Lys Val Lys Ser Val Tyr Ala Leu Thr 165 170 175 Ile Thr Ser Ala Glu Lys Thr Ala Leu Thr Ser Met Leu Asn Thr Cys 180 185 190 <210> 39 <211> 186 <212> PRT <213> Trichoderma reesei <400> 39 Ala Pro Leu Pro Ala Pro Pro Gly Ile Pro Ser Glu Asp Thr Ala Ara 1 5 10 15 Thr Gin Leu Ala Gly Leu Thr Val Ala Val Val Gly Ser Giv Thr Giv 20 25 ao Tyr Ser Arg Asp Leu Phe Pro Thr Trp Asp Ala Ile Ser Giv Asn Cvs 35 40 45 Asn Ala Arg Glu Tyr Val Leu Lys Arg Asp Gly Glu Gly Val Gin Val 50 55 50 Asn Asn Ala Cys Glu Ala Gin Ser Gly Ser Trp lle Ser Pro Tvr Aso 65 70 75 80 Asn Ala Ser Phe Thr Asn Ala Ser Ser Leu Asp Ile Asp His Met Val 85 90 95 Pro Leu Lys Asn Ala Trp Ile Ser Gly Ala Ser Thr Trp Thr Thr Ala 100 105 110 Gin Arg Glu Ala Leu Ala Asn Asp Val Ser Arg Pro Gin Leu Trp Ala 115 120 125 Val Ser Ala Ser Ser Asn Arg Ser Lys Gly Asp Arg Ser Pro Asp Gin 130 135 140 Trp Lys Pro Pro Leu Thr Ser Phe Tyr Cys Thr Tyr Ala Lys Ser Trp 145 150 155 160 Ile Asp Val Lys Ser Tyr Tyr Lys Leu Thr Ile Thr Ser Ala Glu Lys 165 170 175 Thr Ala Leu Ser Ser Met Leu Asp Thr Cys 180 185 <210> 40 <211> 188 <212> PRT <213> Chaetomium thermophilum <4 00> 40 Ala Pro Ala Pro Gin Pro Thr Pro Pro Gly Ile Pro Ser Arg Ser Thr 15 10 15 Ala Gin Ser Tyr Leu Asn Ser Leu Thr Val Ala Ala Ser Tyr Asp Asp 20 25 30 Gly Asn Tyr Asn Arg Asp Leu Phe Pro His Trp Asn Thr Val Ser Gly 35 40 45 Thr Cys Asn Thr Arg Glu Tyr Val Leu Lys Arg Asp Gly Ser Asn Val 50 55 60 Val Thr Asn Ser Ala Cys Gin Ala Thr Ser Gly Thr Trp Tyr Ser Pro 65 70 75 80 Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Ile Asp Ile Asp His 85 90 95 Met Val Pro Leu Lys Asn Ala Trp Ile Ser Gly Ala Asn Thr Trp Ser 100 105 110 Ser Ser Lys Arg Ser Ser Phe Ala Asn Asp Ile Asn Ser Pro Gin Leu 115 120 125 Trp Ala Val Thr Asp Ser Val Asn Gin Ser Lys Gly Asp Lys Ser Pro 130 135 140 Asp Lys Trp Lys Pro Pro Leu Thr Thr Phe Tyr Cys Thr Tyr Ala Lys 145 150 155 160 Ser Trp Ile Thr Val Lys Tyr Asn Tyr Asn Leu Thr Ile Thr Ser Ala 165 170 175 Glu Lys Ser Ala Leu Gin Asn Met Ile Asn Thr Cys 180 185 <210> 41 <211> 190 <212> PRT <213> Scytalidium thermophilum <400> 41 Leu Pro Ala Pro Ala Pro Met Pro Thr Pro Pro Gly Ile Pro Ser Lys 15 10 15 Ser Thr Ala Gin Ser Gin Leu Asn Ala Leu Thr Val Lys Ala Ser Tyr 20 25 30 Asp Asp Gly Lys Tyr Lys Arg Asp Leu Phe Pro His Trp Asn Thr Val 35 40 45 Ser Gly Thr Cys Asn Thr Arg Glu Tyr Val Leu Lys Arg Asp Gly Val 50 55 60 Asn Val Val Thr Asn Ser Ala Cys Ala Ala Thr Ser Gly Thr Trp Tyr 65 70 75 80 Ser Pro Phe Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val Asp Ile 85 90 95 Asp His Met Val Pro Leu Lys Asn Ala Trp Ile Ser Gly Ala Asn Asn 100 105 110 Trp Thr Ser Thr Lys Arg Thr Gin Phe Ala Asn Asp He Asn Leu Pro 115 120 125 Gin Leu Trp Ala Val Thr Asp Asp Val Asn Gin Ala Lys Gly Asp Lys 130 135 140 Ser Pro Asp Lys Trp Lys Pro Pro Leu Thr Ser Phe Tyr Cys Thr Tyr 145 150 155 160 Ala Lys Ser Trp Ile Thr Val Lys Tyr Asn Tyr Gly Leu Ser He Thr 165 170 175 Ser Ala Glu Lys Ser Ala Leu Thr Ser Met lie Asn Thr Cys 180 185 190 <210> 42 <211> 186 <212> PRT <213> Metapochonia suchlasporia <4 00> 42 Val Pro Val Pro Ala Pro Pro Gly lie Pro Ser Thr Ser Thr Ala Lys 1 5 10 15 Thr Leu Leu Ala Gly Leu Lys Val Ala Val Pro Leu Ser Gly Asp Gly 20 25 30 Tyr Ser Arg Glu Lys Phe Pro Leu Trp Glu Thr He Gin Gly Thr Cys 35 40 45 Asn Ala Arg Glu Phe Val Leu Lys Arg Asp Gly Thr Asp Val Lys Thr 50 55 60 Asn Asn Ala Cys Val Ala Glu Ser Gly Asn Trp Val Ser Pro Tyr Asp 65 70 75 80 Gly Val Lys Phe Thr Ala Ala Arg Asp Leu Asp He Asp His Met Val 85 90 95 Pro Leu Lys Asn Ala Trp Ile Ser Gly Ala Ser Gin Trp Thr Thr Glu 100 105 110 Arg Arg Lys Ala Leu Ala Asn Asp Ile Thr Arg Pro Gin Leu Trp Ala 115 120 125 Val Ser Ala His Ala Asn Arg Gly Lys Ser Asp Asp Ser Pro Asp Glu 130 135 140 Trp Lys Pro Pro Leu Lys Thr Phe Trp Cys Thr Tyr Ala Lys Ser Trp 145 150 155 160 Val Gin Val Lys Ser Phe Tyr Glu Leu Thr lie Thr Asp Ala Glu Lys 165 170 175 Gly Ala Leu Ala Gly Met Leu Asp Ser Cys 180 185 <210> 43 <211> 198 <212> PRT <213> Daldinia fissa <4 00> 43 Ala Pro Ala Pro lie Pro Val Ala Glu Pro Ala Pro Met Pro Met Pro 1 5 10 15 Thr Pro Pro Gly lie Pro Ser Ala Ser Ser Ala Lys Ser Gin Leu Ala 20 25 30 Ser Leu Thr Val Lys Ala Ala Val Asp Asp Gly Gly Tyr Gin Arg Asp 35 40 45 Leu Phe Pro Thr Trp Asp Thr lie Thr Gly Thr Cys Asn Thr Arg Glu 50 55 6o Tyr Val Leu Lys Arg Asp Gly Ala Asn Val Gin Val Gly Ser Asp Cvs 65 70 75 80 Tyr Pro Thr Ser Gly Thr Trp Thr Ser Pro Tyr Asp Gly Gly Lys Trp 85 90 95 Thr Ser Pro Ser Asp Val Asp Ile Asp His Met Val Pro Leu Lys Asn 100 105 110 Ala Trp Val Ser Gly Ala Asn Lys Trp Thr Thr Ala Lys Arg Glu Gin 115 120 125 Phe Ala Asn Asp Val Asp Arg Pro Gin Leu Trp Ala Val Thr Asp Asn 130 135 140 Val Asn Ser Ser Lys Gly Asp Lys Ser Pro Asp Thr Trp Lys Pro Pro 145 150 155 160 Leu Thr Ser Phe Tyr Cys Thr Tyr Ala Ser Ala Tyr Val Ala Val Lys 165 170 175 Ser Tyr Trp Gly Leu Thr Ile Thr Ser Ala Glu Lys Ser Ala Leu Ser 180 185 190 Asp Met Leu Gly Thr Cys 195 <210> 44 <211> 188 <212> PRT <213> Acremonium sp.<4 00> 38 Leu Pro Ala Pro Val Thr Leu Glu Ala Arg Ala Pro Pro Asn Ile Pro 1 5 10 15 Thr Thr Ala Ala Ala Lys Thr Gin Leu Ala Gly Leu Thr Val Ala Ala 20 25 30 Gin Gly Pro Gin Thr Gly Tyr Ser Arg Asp Leu Phe Pro His Trp Ile 35 40 45 Thr Gin Ser Gly Ser Cys Asn Thr Arg Glu Val Val Leu Gin Arg Asp 50 55 60 Gly Thr Gly Val Val Thr Asp Ser Ala Cys Ala Ala Thr Ser Gly Ser 65 70 75 80 Trp Tyr Ser Val Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val 85 90 95 Asp Ile Asp His Met Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala 100 105 110 Ala Ser Trp Thr Thr Ala Arg Arg Gin Ala Phe Ala Asn Asp Leu Thr 115 120 125 Asn Pro Gin Leu Leu Ala Val Thr Asp Asn Val Asn Gin Ala Lys Gly 130 135 140 Asp Lys Gly Pro Glu Asp Trp Lys Pro Pro Leu Thr Ser Tyr Tyr Cys 145 150 155 160 Thr Tyr Ala Lys With Trp Val Lys Val Lys Ser Val Tyr Ala Leu Thr 165 170 175 Ile Thr Ser Ala Glu Lys Thr Ala Leu Thr Ser With Leu Asn Thr Cys 180 185 190 <210> 39 <211> 186 <212> PRT <213 > Trichoderma reesei <400> 39 Ala Pro Leu Pro Ala Pro P ro Gly Ile Pro Ser Glu Asp Thr Ala Ara 1 5 10 15 Thr Gin Leu Ala Gly Leu Thr Val Ala Val Val Gly Ser Give Thr Give 20 25 ao Tyr Ser Arg Asp Leu Phe Pro Thr Trp Asp Ala Ile Ser Give Asn Cvs 35 40 45 Asn Ala Arg Glu Tyr Val Leu List Arg Asp Gly Glu Gly Val Gin Val 50 55 50 Asn Asn Ala Cys Glu Ala Gin Ser Gly Ser Trp lle Ser Pro Tvr Aso 65 70 75 80 Asn Ala Ser Phe Thr Asn Ala Ser Ser Leu Asp Ile Asp His With Val 85 90 95 Pro Leu Lys Asn Ala Trp Ile Ser Gly Ala Ser Thr Trp Thr Thr Ala 100 105 110 Gin Arg Glu Ala Leu Ala Asn Asp Val Ser Arg Pro Gin Leu Trp Ala 115 120 125 Val Ser Ala Ser Ser Asn Arg Ser Lys Gly Asp Arg Ser Pro Asp Gin 130 135 140 Trp Lys Pro Pro Leu Thr Ser Phe Tyr Cys Thr Tyr Ala Lys Ser Trp 145 150 155 160 Ile Asp Val Lys Ser Tyr Tyr Lys Leu Thr Ile Thr Ser Ala Glu List 165 170 175 Thr Ala Leu Ser Ser With Leu Asp Thr Cys 180 185 <210> 40 <211> 188 <212> PRT <213> Chaetomium thermophilum <400> 40 Ala Pro Ala Pro Gin Pro Thr Pro Pro Gly Ile Pro Ser Arg Ser Thr 15 10 15 Ala Gin S is Tyr Leu Asn Ser Leu Thr Val Ala Ala Ser Tyr Asp Asp 20 25 30 Gly Asn Tyr Asn Arg Asp Leu Phe Pro His Trp Asn Thr Val Ser Gly 35 40 45 Thr Cys Asn Thr Arg Glu Tyr Val Leu Lys Arg Asp Gly Ser Asn Val 50 55 60 Val Thr Asn Ser Ala Cys Gin Ala Thr Ser Gly Thr Trp Tyr Ser Pro 65 70 75 80 Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Ile Asp Ile Asp His 85 90 95 With Val Pro Leu Lys Asn Ala Trp Ile Ser Gly Ala Asn Thr Trp Ser 100 105 110 Ser Ser Lys Arg Ser Ser Phe Ala Asn Asp Ile Asn Ser Pro Gin Leu 115 120 125 Trp Ala Val Thr Asp Ser Val Asn Gin Ser Lys Gly Asp Lys Ser Pro 130 135 140 Asp Lys Trp Lys Pro Pro Leu Thr Thr Phe Tyr Cys Thr Tyr Ala Lys 145 150 155 160 Ser Trp Ile Thr Val Lys Tyr Asn Tyr Asn Leu Thr Ile Thr Ser Ala 165 170 175 Glu Lys Ser Ala Leu Gin Asn With Ile Asn Thr Cys 180 185 <210> 41 <211> 190 <212> PRT <213> Scytalidium thermophilum <400> 41 Leu Pro Ala Pro Ala Pro With Pro Thr Pro Pro Gly Ile Pro Ser List 15 10 15 Ser Thr Ala Gin Ser Gin Leu Asn Ala Leu Thr Val Lys Ala Ser Tyr 20 25 30 Asp Asp Gly Lys Tyr Lys Arg Asp Leu Phe Pro His Trp Asn Thr Val 35 40 45 Ser Gly Thr Cys Asn Thr Arg Glu Tyr Val Leu Lys Arg Asp Gly Val 50 55 60 Asn Val Val Thr Asn Ser Ala Cys Ala Ala Thr Ser Gly Thr Trp Tyr 65 70 75 80 Ser Pro Phe Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val Asp Ile 85 90 95 Asp His With Val Pro Leu Lys Asn Ala Trp Ile Ser Gly Ala Asn Asn 100 105 110 Trp Thr Ser Thr Lys Arg Thr Gin Phe Ala Asn Asp He Asn Leu Pro 115 120 125 Gin Leu Trp Ala Val Thr Asp Asp Val Asn Gin Ala Lys Gly Asp Lys 130 135 140 Ser Pro Asp Lys Trp Lys Pro Pro Leu Thr Ser Phe Tyr Cys Thr Tyr 145 150 155 160 Ala Lys Ser Trp Ile Thr Val Lys Tyr Asn Tyr Gly Leu Ser He Thr 165 170 175 Ser Ala Glu Lys Ser Ala Leu Thr Ser With lie Asn Thr Cys 180 185 190 <210> 42 <211 > 186 <212> PRT <213> Metapochonia suchlasporia <4 00> 42 Val Pro Val Pro Ala Pro Pro Gly lie Pro Ser Thr Ser Thr Ala Lys 1 5 10 15 Thr Leu Leu Ala Gly Leu Lys Val Ala Val Pro Leu Ser Gly Asp Gly 20 25 30 Tyr Ser Arg Glu Lys Phe Pro Leu Trp Glu Thr He Gin Gly Thr Cys 35 40 45 Asn Ala Arg Glu Phe Val Leu Lys Arg Asp Gly Thr Asp Val Lys Thr 50 55 60 Asn Asn Ala Cys Val Ala Glu Ser Gly Asn Trp Val Ser Pro Tyr Asp 65 70 75 80 Gly Fall List Phe Thr Ala Ala Arg Asp Leu Asp He Asp His With Val 85 90 95 Pro Leu List Asn Ala Trp Ile Ser Gly Ala Ser Gin Trp Thr Thr Glu 100 105 110 Arg Arg List Ala Leu Ala Asn Asp Ile Thr Arg Pro Gin Leu Trp Ala 115 120 125 Val Ser Ala His Ala Asn Arg Gly Lys Ser Asp Asp Ser Pro Asp Glu 130 135 140 Trp List Pro Pro Leu Lys Thr Phe Trp Cys Thr Tyr Ala Lys Ser Trp 145 150 155 160 Val Gin Val Lys Ser Phe Tyr Glu Leu Thr lie Thr Asp Ala Glu Lys 165 170 175 Gly Ala Leu Ala Gly With Leu Asp Ser Cys 180 185 <210> 43 <211> 198 <212> PRT <213> Daldinia fissa <4 00> 43 Ala Pro Ala Pro lie Pro Val Ala Glu Pro Ala Pro Met Pro With Pro 1 5 10 15 Thr Pro Pro Gly lie Pro Ser Ala Ser Ser Ala Lys Ser Gin Leu Ala 20 25 30 Ser Leu Thr Val Lys Ala Ala Val Asp Asp Gly Gly Tyr Gin Arg Asp 35 40 45 Leu Phe Pro Thr Tr p Asp Thr lie Thr Gly Thr Cys Asn Thr Arg Glu 50 55 6o Tyr Val Leu Lys Arg Asp Gly Ala Asn Val Gin Val Gly Ser Asp Cvs 65 70 75 80 Tyr Pro Thr Ser Gly Thr Trp Thr Ser Pro Tyr Asp Gly Gly Lys Trp 85 90 95 Thr Ser Pro Ser Asp Val Asp Ile Asp His With Val Pro Leu Lys Asn 100 105 110 Ala Trp Val Ser Gly Ala Asn Lys Trp Thr Thr Ala Lys Arg Glu Gin 115 120 125 Phe Ala Asn Asp Val Asp Arg Pro Gin Leu Trp Ala Val Thr Asp Asn 130 135 140 Val Asn Ser Ser Lys Gly Asp Lys Ser Pro Asp Thr Trp Lys Pro Pro 145 150 155 160 Leu Thr Ser Phe Tyr Cys Thr Tyr Ala Ser Ala Tyr Val Ala Val Lys 165 170 175 Ser Tyr Trp Gly Leu Thr Ile Thr Ser Ala Glu List Ser Ala Leu Ser 180 185 190 Asp With Leu Gly Thr Cys 195 <210> 44 <211> 188 <212> PRT <213> Acremonium sp. XZ2007 <4 00> 44 Leu Pro Leu Gin Ser Arg Asp Pro Pro Gly Ile Pro Ser Thr Ala Thr 15 10 15 Ala Lys Ser Leu Leu Asn Gly Leu Thr Val Lys Ala Trp Ser Asn Glu 20 25 30 Gly Thr Tyr Asp Arg Asp Leu Phe Pro His Trp Gin Thr Ile Glu Gly 35 40 45 Thr Cys Asn Ala Arg Glu Tyr Val Leu Lys Arg Asp Gly Gin Asn Val 50 55 60 Val Val Asn Ser Ala Cys Thr Ala Gin Ser Gly Thr Trp Lys Ser Val 65 70 75 80 Tyr Asp Gly Glu Thr Thr Asn Ser Ala Ser Asp Leu Asp Ile Asp His 85 90 95 Met Ile Pro Leu Lys Asn Ala Trp Ile Ser Gly Ala Ala Thr Trp Thr 100 105 110 Thr Ala Gin Arg Thr Ser Phe Ala Asn Asp Ile Ser Ser Pro Gin Leu 115 120 125 Trp Ala Val Thr Ala Gly Val Asn Arg Ser Lys Ser Asp Arg Ser Pro 130 135 140 Asp Thr Trp Val Pro Pro Leu Ala Ser Phe His Cys Thr Tyr Gly Lys 145 150 155 160 Ala Trp Val Gin Val Lys Ser Lys Trp Ala Leu Ser Ile Thr Ser Ala 165 170 175 Glu Lys Ser Ala Leu Thr Gly Leu Leu Asn Lys Cys 180 185 <210> 45 <211> 182 <212> PRT <213> Acremonium dichromosporum <4 00> 45 Ile Pro Pro Gly Ile Pro Ser Glu Ala Thr Ala Arg Ser Leu Leu Ser 15 10 15 Ser Leu Thr Val Ala Pro Thr Val Asp Asp Gly Thr Tyr Asp Arg Asp 20 25 30 Leu Phe Pro His Trp Ser Ser Val Glu Gly Asn Cys Asn Ala Arg Glu 35 40 45 Phe Val Leu Arg Arg Asp Gly Asp Gly Val Ser Val Gly Asn Asp Cys 50 55 go Tyr Pro Thr Ala Gly Thr Trp Thr Cys Pro Tyr Asp Gly Lys Arg His 65 70 75 80 Ser Val Pro Ser Asp Val Ser Ile Asp His Met Val Pro Leu His Asn 85 90 95 Ala Trp Met Thr Gly Ala Ser Glu Trp Thr Thr Ala Glu Arg Glu Ala 100 105 HO Phe Ala Asn Asp lie Asp Gly Pro Gin Leu Trp Ala Val Thr Ser Thr 115 120 125 Thr Asn Ser Gin Lys Gly Ser Asp Ala Pro Asp Glu Trp Gin Pro Pro 130 135 140 Gin Thr Ser Ile His Cys Lys Tyr Ala Ala Ala Trp Ile Gin Val Lys 145 150 155 160 Ser Thr Tyr Asp Leu Thr Val Ser Ser Ala Glu Gin Ala Ala Leu Glu 165 170 175 Glu Met Leu Gly Arg Cys 180 <210> 46 <211> 188 <212> PRT <213> Sarocladium sp.XZ2007 <4 00> 44 Leu Pro Leu Gin Ser Arg Asp Pro Pro Gly Ile Pro Ser Thr Ala Thr 15 10 15 Ala Lys Ser Leu Leu Asn Gly Leu Thr Val Lys Ala Trp Ser Asn Glu 20 25 30 Gly Thr Tyr Asp Arg Asp Leu Phe Pro His Trp Gin Thr Ile Glu Gly 35 40 45 Thr Cys Asn Ala Arg Glu Tyr Val Leu Lys Arg Asp Gly Gin Asn Val 50 55 60 Val Val Asn Ser Ala Cys Thr Ala Gin Ser Gly Thr Trp Lys Ser Val 65 70 75 80 Tyr Asp Gly Glu Thr Thr Asn Ser Ala Ser Asp Leu Asp Ile Asp His 85 90 95 With Ile Pro Leu Lys Asn Ala Trp Ile Ser Gly Ala Ala Thr Trp Thr 100 105 110 Thr Ala Gin Arg Thr Ser Phe Ala Asn Asp Ile Ser Ser Pro Gin Leu 115 120 125 Trp Ala Val Thr Ala Gly Val Asn Arg Ser Lys Ser Asp Arg Ser Pro 130 135 140 Asp Thr Trp Val Pro Pro Leu Ala Ser Phe His Cys Thr Tyr Gly Lys 145 150 155 160 Ala Trp Val Gin Val Lys Ser Lys Trp Ala Leu Ser Ile Thr Ser Ala 165 170 175 Glu Lys Ser Ala Leu Thr Gly Leu Leu Asn Lys Cys 180 185 <210> 45 <211> 182 <212> PRT <213> Acremonium dichromosporum <4 00> 45 Ile Pro Pro Gly Ile Pro Ser Glu Ala Thr Ala Arg Ser Leu Leu Ser 15 10 15 Ser Leu Thr Val Ala Pro Thr Val Asp Asp Gly Thr Tyr Asp Arg Asp 20 25 30 Leu Phe Pro His Trp Ser Ser Val Glu Gly Asn Cys Asn Ala Arg Glu 35 40 45 Phe Val Leu Arg Arg Asp Gly Asp Gly Val Ser Val Gly Asn Asp Cys 50 55 go Tyr Pro Thr Ala Gly Thr Trp Thr Cys Pro Tyr Asp Gly List Arg His 65 70 75 80 Ser Val Pro Ser Asp Val Ser Ile Asp His With Val Pro Leu His Asn 85 90 95 Ala Trp With Thr Gly Ala Ser Glu Trp Thr Thr Ala Glu Arg Glu Ala 100 105 HO Phe Ala Asn Asp lie Asp Gly Pro Gin Leu Trp Ala Val Thr Ser Thr 115 120 125 Thr Asn Ser Gin Lys Gly Ser Asp Ala Pro Asp Glu Trp Gin Pro Pro 130 135 140 Gin Thr Ser Ile His Cys Lys Tyr Ala Ala Ala Trp Ile Gin Val Lys 145 150 155 160 Ser Thr Tyr Asp Leu Thr Val Ser Ser Ala Glu Gin Ala Ala Leu Glu 165 170 175 Glu With Leu Gly Arg Cys 180 <210> 46 <211> 188 <212> PRT <213> Sarocladium sp. XZ2014 <4 00> 46 Val Pro lie Pro Leu Pro Asp Pro Pro Gly lie Pro Ser Ser Ser Thr 1 5 10 15 Ala Asn Thr Leu Leu Ala Gly Leu Thr Val Arg Ala Ser Ser Asn Glu 20 25 30 Asp Thr Tyr Asn Arg Asp Leu Phe Pro His Trp Val Ala He Ser Gly 35 40 45 Asn Cys Asn Ala Arg Glu Tyr Val Leu Arg Arg Asp Gly Thr Asn Val 50 55 60 Val Val Asn Thr Ala Cys Val Pro Gin Ser Gly Thr Trp Arg Ser Pro 65 70 75 80 Tyr Asp Gly Glu Ser Thr Thr Asn Ala Ser Asp Leu Asp lie Asp His 85 90 95 Met Val Pro Leu Lys Asn Ala Trp Ile Ser Gly Ala Ala Ser Trp Thr 100 105 HO Thr Ala Lys Arg Gin Asp Phe Ala Asn Asp Val Ser Gly Pro Gin Leu H5 120 125 Trp Ala Val Thr Ala Gly Val Asn Arg Ser Lys Gly Asp Lys Ser Pro 130 135 140 Asp Ser Trp Val Pro Pro Leu Ala Ser Phe His Cys Thr Tyr Ala Arg 145 150 155 160 Ser Trp lie Gin Val Lys Ser Ser Trp Ala Leu Ser Val Thr Ser Ala 165 170 175 Glu Lys Ala Ala Leu Thr Asp Leu Leu Ser Thr Cys 180 185 <210> 47 <211> 186 <212> PRT <213> Metarhizium sp.XZ2014 <4 00> 46 Val Pro lie Pro Leu Pro Asp Pro Gly lie Pro Ser Ser Ser Thr 1 5 10 15 Ala Asn Thr Leu Leu Ala Gly Leu Thr Val Arg Ala Ser Ser Asn Glu 20 25 30 Asp Thr Tyr Asn Arg Asp Leu Phe Pro His Trp Val Ala He Ser Gly 35 40 45 Asn Cys Asn Ala Arg Glu Tyr Val Leu Arg Arg Asp Gly Thr Asn Val 50 55 60 Val Val Asn Thr Ala Cys Val Pro Gin Ser Gly Thr Trp Arg Ser Pro 65 70 75 80 Tyr Asp Gly Glu Ser Thr Thr Asn Ala Ser Asp Leu Asp lie Asp His 85 90 95 With Val Pro Leu Lys Asn Ala Trp Ile Ser Gly Ala Ala Ser Trp Thr 100 105 HO Thr Ala Lys Arg Gin Asp Phe Ala Asn Asp Val Ser Gly Pro Gin Leu H5 120 125 Trp Ala Val Thr Ala Gly Val Asn Arg Ser Lys Gly Asp Lys Ser Pro 130 135 140 Asp Ser Trp Val Pro Pro Leu Ala Ser Phe His Cys Thr Tyr Ala Arg 145 150 155 160 Ser Trp lie Gin Val Lys Ser Ser Trp Ala Leu Ser Val Thr Ser Ala 165 170 175 Glu Lys Ala Ala Leu Thr Asp Leu Leu Ser Thr Cys 180 185 <210> 47 <211> 186 <212> PRT <213> Metarhizium sp. HNA15-2 <4 00> 47 Val Pro Val Pro Ala Pro Pro Gly He Pro Thr Ala Ser Thr Ala Arg 1 5 10 15 Thr Leu Leu Ala Gly Leu Lys Val Ala Thr Pro Leu Ser Gly Asp Gly 20 25 30 Tyr Ser Arg Thr Leu Phe Pro Thr Trp Glu Thr lie Glu Gly Thr Cys 35 40 45 Asn Ala Arg Glu Phe Val Leu Lys Arg Asp Gly Thr Asp Val Gin Thr 50 55 60 Asn Thr Ala Cys Val Ala Gin Ser Gly Asn Trp Val Ser Pro Tyr Asp 65 70 75 80 Gly Val Ala Phe Thr Ala Ala Ser Asp Leu Asp He Asp His Met Val 85 90 95 Pro Leu Lys Asn Ala Trp Ile Ser Gly Ala Ser Gin Trp Thr Thr Asp 100 105 110 Lys Arg Lys Gly Leu Ala Asn Asp Ile Thr Arg Pro Gin Leu Trp Ala 115 120 125 Val Ser Ala His Ala Asn Arg Ala Lys Gly Asp Ser Ser Pro Asp Glu 130 135 140 Trp Lys Pro Pro Leu Lys Thr Phe Trp Cys Thr Tyr Ala Arg Ser Trp 145 150 155 160 Val Gin Val Lys Ser Tyr Tyr Ala Leu Thr lie Thr Asp Ala Glu Lys 165 170 175 Gly Ala Leu Ser Gly Met Leu Asp Ser Cys 180 185 <210> 48 <211> 188 <212> PRT <213> Acremonium sp. XZ2414 <4 00> 48 Ala Pro lie Ala Val Arg Asp Pro Pro Gly lie Pro Ser Ala Ser Thr 15 10 15 Ala Asn Thr Leu Leu Ala Gly Leu Thr Val Arg Ala Ser Ser Asn Glu 20 25 30 Asp Ser Tyr Asp Arg Asn Leu Phe Pro His Trp Ser Ala He Ser Gly 35 40 45 Asn Cys Asn Ala Arg Glu Phe Val Leu Glu Arg Asp Gly Thr Asn Val 50 55 60 Val Val Asn Asn Ala Cys Val Ala Gin Ser Gly Thr Trp Arg Ser Pro 65 70 75 80 Tyr Asp Gly Glu Thr Thr Gly Asn Ala Ser Asp Leu Asp lie Asp His 85 90 95 Met Val Pro Leu Lys Asn Ala Trp Ile Ser Gly Ala Ser Ser Trp Ser 100 105 no Thr Thr Arg Arg Gin Glu Phe Ala Asn Asp Val Ser Gly Pro Gin Leu 115 120 125 Trp Ala Val Thr Ala Gly Val Asn Arg Ser Lys Gly Asp Arg Ser Pro 130 135 140 Asp Ser Trp Val Pro Pro Leu Ala Ser Phe His Cys Thr Tyr Ala Lys 145 150 155 160 Ser Trp Val Gin Val Lys Ser Ser Trp Ser Leu Ser Val Thr Ser Ala 165 170 175 Glu Lys Ala Ala Leu Ser Asp Leu Leu Gly Thr Cys 180 185 <210> 49 <211> 186 <212> PRT <213> Isaria tenuipes <4 00> 49 Ala Pro Val Pro Glu Pro Pro Gly lie Pro Ser Thr Ser Thr Ala Gin 15 10 15 Ser Asp Leu Asn Ser Leu Gin Val Ala Ala Ser Gly Ser Gly Asp Gly 20 25 30 Tyr Ser Arg Ala Glu Phe Pro His Trp Val Ser Val Glu Gly Ser Cys 35 40 45 Asp Ser Arg Glu Tyr Val Leu Lys Arg Asp Gly Gin Asp Val Gin Ala 50 55 60 Asp Ser Ser Cys Lys He Thr Ser Gly Thr Trp Val Ser Pro Tyr Asp 65 70 75 80 Ala Thr Thr Trp Thr Asn Ser Ser Lys Val Asp lie Asp His Leu Val 85 90 95 Pro Leu Lys Asn Ala Trp lie Ser Gly Ala Ser Ser Trp Thr Lys Ala 100 105 110 Gin Arg Gin Asp Phe Ala Asn Asp Ile Lys Arg Pro Gin Leu Tyr Ala 115 120 125 Val Ser Glu Asn Ala Asn Arg Ser Lys Gly Asp Arg Ser Pro Asp Gly 130 135 140 Trp Lys Pro Pro Leu Lys Ser Phe Tyr Cys Thr Tyr Ala Lys Ser Trp 145 150 155 160 Val Ala Val Lys Ser Tyr Tyr Lys Leu Thr He Thr Ser Ala Glu Lys 165 170 175 Ser Ala Leu Gly Asp Met Leu Asp Thr Cys 180 185 <210> 50 <211> 184 <212> PRT <213> Scytalidium circinatum <4 00> 50 Ala Pro Pro Gly lie Pro Ser Ala Ser Thr Ala Ser Ser Leu Leu Gly 1 5 10 15 Glu Leu Ala Val Ala Glu Pro Val Asp Asp Gly Ser Tyr Asp Arg Aso 20 25 30 Leu Phe Pro His Trp Glu Pro He Pro Gly Glu Thr Ala Cys Ser Ala 35 40 45 Arg Glu Tyr Val Leu Arg Arg Asp Gly Thr Gly Val Glu Thr Gly Ser 50 55 60 Asp Cys Tyr Pro Thr Ser Gly Thr Trp Ser Ser Pro Tyr Asp Gly Gly 65 70 75 80 Ser Trp Thr Ala Pro Ser Asp Val Asp lie Asp His Met Val Pro Leu 85 90 95 Lys Asn Ala Trp He Ser Gly Ala Ser Glu Trp Thr Thr Ala Glu Arq 100 105 440 Glu Ala Phe Ala Asn Asp He Asp Gly Pro Gin Leu Trp Ala Val Thr 115 120 125 Asp Glu Val Asn Gin Ser Lys Ser Asp Gin Ser Pro Asp Glu Trp Lys 130 135 140 Pro Pro Leu Ser Ser Phe Tyr Cys Thr Tyr Ala Cys Ala Trp lie Gin 145 150 155 160 Val Lys Ser Thr Tyr Ser Leu Ser He Ser Ser Ala Glu Gin Ala Ala 165 170 175 Leu Glu Asp Met Leu Gly Ser Cys 180 <210> 51 <211> 186 <212> PRT <213> Metarhizium lepidiotae <4 00> 51 Val Pro Val Pro Ala Pro Pro Gly lie Pro Thr Ala Ser Thr Ala Arg 1 5 10 15 Thr Leu Leu Ala Gly Leu Lys Val Ala Thr Pro Leu Ser Gly Asp Gly 20 25 30 Tyr Ser Arg Thr Leu Phe Pro Thr Trp Glu Thr He Glu Gly Thr Cys 35 40 45 Asn Ala Arg Glu Phe Val Leu Lys Arg Asp Gly Thr Asp Val Gin Thr 50 55 60 Asn Thr Ala Cys Val Ala Glu Ser Gly Asn Trp Val Ser Pro Tyr Asp 65 70 75 80 Gly Val Ser Phe Thr Ala Ala Ser Asp Leu Asp He Asp His Met Val 85 90 95 Pro Leu Lys Asn Ala Trp He Ser Gly Ala Ser Gin Trp Thr Thr Asp 100 105 110 Lys Arg Lys Asp Leu Ala Asn Asp Ile Thr Arg Pro Gin Leu Trp Ala 115 120 125 Val Ser Ala His Ala Asn Arg Ser Lys Gly Asp Ser Ser Pro Asp Glu 130 135 140 Trp Lys Pro Pro Leu Gin Thr Phe Trp Cys Thr Tyr Ser Lys Ser Trp 145 150 155 160 Ile Gin Val Lys Ser His Tyr Ser Leu Thr Ile Thr Asp Ala Glu Lys 165 170 175 Gly Ala Leu Ser Gly Met Leu Asp Ser Cys 180 185 <210> 52 <211> 226 <212> PRT <213> Thermobispora bispora <4 00> 52 Leu Asp Ile Ala Asp Gly Arg Pro Ala Gly Gly Lys Ala Ala Glu Ala 1 5 10 15 Ala Thr Gly Thr Ser Pro Leu Ala Asn Pro Asp Gly Thr Arg Pro Gly 20 25 30 Leu Ala Ala Ile Thr Ser Ala Asp Glu Arg Ala Glu Ala Arg Ala Leu 35 40 45 Ile Glu Arg Leu Arg Thr Lys Gly Arg Gly Pro Lys Thr Gly Tyr Glu 50 55 60 Arg Glu Lys Phe Gly Tyr Ala Trp Ala Asp Ser Val Asp Gly Ile Pro 70 75 80 Phe Gly Arg Asn Gly Cys Asp Thr Arg Asn Asp Val Leu Lys Arg Asp 85 90 95 Gly Gin Arg Leu Gin Phe Arg Ser Gly Ser Asp Cys Val Val Ile Ser 100 105 110 Met Thr Leu Phe Asp Pro Tyr Thr Gly Lys Thr Ile Glu Trp Thr Lys 115 120 125 Gin Asn Ala Ala Glu Val Gin Ile Asp His Val Val Pro Leu Ser Tyr 130 135 140 Ser Trp Gin Met Gly Ala Ser Arg Trp Ser Asp Glu Lys Arg Arg Gin 145 150 155 160 Leu Ala Asn Asp Pro Leu Asn Leu Met Pro Val Asp Gly Ala Thr Asn 165 170 175 Ser Arg Lys Gly Asp Ser Gly Pro Ala Ser Trp Leu Pro Pro Arg Arg 180 185 190 Glu He Arg Cys Ala Tyr Val Val Arg Phe Ala Gin Val Ala Leu Lys 195 200 205 Tyr Asp Leu Pro Val Thr Thr Ala Asp Lys Glu Thr Met Leu Gin Gin 210 215 220 Cys Ser 225 <210> 53 <211> 191 <212> PRT <213> Sporormia fimetaria <4 00> 53 Leu Pro Ala Pro Val Leu Glu Lys Arg Thr Pro Pro Asn He Pro Ser 15 10 15 Thr Ser Thr Ala Gin Ser Leu Leu Ser Gly Leu Thr Val Ala Pro Gin 20 25 30 Gly Ser Gin Thr Gly Tyr Ser Arg Asp Leu Phe Pro His Trp He Thr 35 40 45 Val Ser Gly Thr Cys Asn Thr Arg Glu Thr Val Leu Lys Arg Asp Gly 50 55 60 Ser Asn Val Val Thr Asp Ser Ala Cys Ala Ser Val Ser Gly Ser Trp 65 70 75 80 Tyr Ser Thr Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val Asp 85 90 95 Ile Asp His Val Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala Ala 100 105 110 Ser Trp Thr Thr Ala Arg Arg Gin Ala Phe Ala Asn Asp Leu Thr Asn 115 120 125 Pro Gin Leu Ile Ala Val Thr Asp Asn Val Asn Gin Ala Lys Gly Asp 130 135 140 Gin Gly Pro Glu Ser Trp Lys Pro Pro Leu Thr Ser Tyr Tyr Cys Thr 145 150 155 160 Tyr Ala Lys Met Trp Val Lys Val Lys Ser Val Tyr Ser Leu Thr Val 165 170 175 Thr Ser Ala Glu Lys Ser Ala Leu Ser Ser Met Leu Gly Thr Cys 180 185 190 <210> 54 <211> 193 <212> PRT <213> Pycnidiophora cf.dispera <4 00> 54 Leu Pro Ala Pro Ala Pro Val Leu Val Ala Arg Glu Pro Pro Asn Ile 1 5 10 15 Pro Ser Thr Ser Ser Ala Gin Ser Met Leu Ser Gly Leu Thr Val Lys 20 25 30 Ala Gin Gly Pro Gin Asp Gly Tyr Ser Arg Asp Leu Phe Pro His Trp 35 40 45 Ile Thr Ile Ser Gly Thr Cys Asn Thr Arg Glu Thr Val Leu Lys Arg 50 55 60 Asp Gly Thr Asn Val Val Thr Asn Ser Ala Cys Ala Ser Thr Ser Gly 65 70 75 80 Ser Trp Tyr Ser Pro Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp 85 90 95 Val Asp Ile Asp His Ile Val Pro Leu Ser Asn Ala Trp Lys Ser Gly 100 105 110 Ala Ala Ser Trp Thr Thr Ser Arg Arg Gin Gin Phe Ala Asn Asp Leu 115 120 125 Thr Asn Pro Gin Leu Ile Ala Val Thr Asp Ser Val Asn Gin Ala Lys 130 135 140 Gly Asp Lys Gly Pro Glu Asp Trp Lys Pro Ser Arg Thr Ser Tyr His 145 150 155 160 Cys Thr Tyr Ala Lys Met Trp Ile Lys Val Lys Ser Val Tyr Ser Leu 165 170 175 Thr Val Thr Ser Ala Glu Lys Ser Ala Leu Thr Thr Met Leu Asn Thr 180 185 190 Cys <210> 55 <211> 199 <212> PRT <213> Enviromental sample D <4 00> 55 Asp Thr Asp Pro Glu Pro Val Ala Gly Ser Ala Leu Glu Ala Leu Ala 15 10 15 Gly Leu Glu Val Lys Gly Pro Gly Pro Asp Thr Gly Tyr Glu Arg Ala 20 25 30 Leu Phe Gly Pro Pro Trp Ala Asp Val Asp Gly Asn Gly Cys Asp Thr 35 40 45 Arg Asn Asp Ile Leu Ala Arg Asp Leu Thr Asp Leu Thr Phe Ser Thr 50 55 60 Arg Gly Asp Val Cys Glu Val Arg Thr Gly Thr Phe Asp Asp Pro Tyr 65 70 75 80 Thr Gly Glu Thr Ile Asp Phe Arg Arg Gly Asn Ala Thr Ser Ala Ala 85 90 95 Val Gin Ile Asp His Val Val Pro Leu Leu Asp Ala Trp Arg Lys Gly 100 105 110 Ala Arg Ala Trp Asp Asp Glu Thr Arg Arg Gin Phe Ala Asn Asp Pro 115 120 125 Leu Asn Leu Leu Ala Ser Asp Gly Pro Ala Asn Gin Ser Lys Gly Ala 130 135 140 Arg Asp Ala Ser Ala Trp Leu Pro Pro Asn His Ala Phe Arg Cys Pro 145 150 155 160 Tyr Val Ala Arg Gin Ile Ala Val Lys Ala Ala Tyr Glu Leu Ser Val 165 170 175 Thr Pro Ser Glu Ser Glu Ala Met Ala Arg Val Leu Ala Asp Cys Pro 180 185 190 Ala Glu Pro Leu Pro Ala Gly 195 <210> 56 <211> 199 <212> PRT <213> Enviromental sample O <4 00> 56 Asp Asp Glu Pro Glu Pro Ala Arg Gly Ser Ala Leu Glu Ala Leu Ala 1 5 10 15 Arg Leu Glu Val Val Gly Pro Gly Pro Asp Thr Gly Tyr Glu Arg Glu 20 25 30 Leu Phe Gly Pro Ala Trp Ala Asp Val Asp Gly Asn Gly Cys Asp Thr 35 40 45 Arg Asn Asp Ile Leu Ala Arg Asp Leu Thr Asp Leu Thr Phe Ser Thr 50 55 60 Arg Gly Glu Val Cys Glu Val Arg Thr Gly Thr Phe Gin Asp Pro Tyr 65 70 75 80 Thr Gly Glu Thr Ile Asp Phe Arg Arg Gly Asn Ala Thr Ser Met Ala 85 90 95 Val Gin Ile Asp His Val Val Pro Leu Met Asp Ala Trp Arg Lys Gly 100 105 110 Ala Arg Ala Trp Asp Asp Glu Thr Arg Arg Gin Phe Ala Asn Asp Pro 115 120 125 Leu Asn Leu Leu Ala Ser Asp Gly Pro Ala Asn Gin Ser Lys Gly Ala 130 135 140 Arg Asp Ala Ser Ala Trp Leu Pro Pro Asn His Ala Phe Arg Cys Pro 145 150 155 160 Tyr Val Ala Arg Gin Ile Ala Val Lys Thr Ala Tyr Glu Leu Ser Val 165 170 175 Thr Pro Ser Glu Ser Glu Ala Met Ala Arg Val Leu Glu Asp Cys Pro 180 185 190 Ala Glu Pro Val Pro Ala Gly 195 <210> 57 <211> 186 <212> PRT <213> Clavicipitaceae sp-70249 <4 00> 57 Val Pro Val Pro Ala Pro Pro Gly ile Pro Ser Thr Ser Thr Ala Lys 1 5 10 15 Thr Leu Leu Ala Gly Leu Lys Val Ala Thr Pro Leu Ser Gly Asp Gly 20 25 30 Tyr Ser Arg Asp Lys Phe Pro Thr Trp Glu Thr Ile Gin Gly Thr Cys 35 40 45 Asn Ala Arg Glu Phe Val Ile Lys Arg Asp Gly Thr Asp Val Lys Thr 50 55 60 Asn Ser Ala Cys Val Ala Glu Ser Gly Asn Trp Val Ser Pro Tyr Asp 65 70 75 80 Gly Val Lys Phe Thr Ala Ala Arg Asp Leu Asp Ile Asp His Met Val 85 90 95 Pro Leu Lys Asn Ala Trp Ile Ser Gly Ala Ser Gin Trp Thr Thr Glu 100 105 110 Gin Arg Lys Ala Leu Ala Asn Asp Ile Thr Arg Pro Gin Leu Trp Ala 115 120 125 Val Ser Ala His Ala Asn Arg Gly Lys Ser Asp Asp Ser Pro Asp Glu 130 135 140 Trp Lys Pro Pro Leu Lys Thr Phe Trp Cys Thr Tyr Ala Lys Ser Trp 145 150 155 160 Val Gin Val Lys Ser Phe Tyr Lys Leu Thr Ile Thr Asp Thr Glu Lys 165 170 175 Gly Ala Leu Ala Gly Met Leu Asp Thr Cys 180 185 <210> 58 <211> 187 <212> PRT <213> Westerdykella sp. AS85-2 <400> 58 Phe Pro Ala Pro Ala Ser Val Leu Glu Ala Arg Ala Pro Pro Asn Ile 15 10 15 Pro Ser Ala Ser Thr Ala Gin Ser Leu Leu Val Gly Leu Thr Val Gin 20 25 30 Pro Gin Gly Pro Gin Asp Gly Tyr Ser Arg Asp Leu Phe Pro His Trp 35 40 45 Ile Thr Ile Ser Gly Thr Cys Asn Thr Arg Glu Thr Val Leu Lys Arg 50 55 60 Asp Gly Ser Asn Val Val Thr Asn Ser Ala Cys Ala Ala Thr Ser Gly 65 70 75 80 Thr Trp Tyr Ser Pro Tyr Asp Gly Ala Thr Trp Thr Ser Ala Ser Asp 85 90 95 Val Asp Ile Asp His Leu Val Pro Leu Ser Asn Ala Trp Lys Ser Gly 100 105 110 Ala Ala Ser Trp Thr Thr Ala Lys Arg Gin Gin Phe Ala Asn Asp Leu 115 120 125 Thr Asn Pro Gin Leu Leu Ala Val Thr Asp Arg Val Asn Gin Ala Lys 130 135 140 Gly Asp Lys Gly Pro Glu Ala Trp Lys Pro Ser Leu Ala Ser Tyr His l45 150 155 160 Cys Thr Tyr Ala Lys Met Trp Val Lys Val Lys Ser Lys Asp Val Arg 165 170 175 Leu Thr Gly Asn Trp Thr Lys Asp Asp Gly Trp 180 185 <210> 59 <211> 194 <212> PRT <213> Humicolopsis cephalosporioides <4 00> 59 Ala Pro Thr Pro Ala Pro Val Glu Leu Glu Arg Arg Thr Pro Pro Asn 15 10 15 Ile Pro Thr Thr Ala Ser Ala Lys Ser Leu Leu Ala Gly Leu Thr Val 20 25 30 Ala Ala Gin Gly Pro Gin Thr Gly Tyr Ser Arg Asp Leu Phe Pro His 35 40 45 Trp Ile Thr Ile Ser Gly Ser Cys Asn Thr Arg Glu Thr Val Leu Lys 50 55 60 Arg Asp Gly Thr Gly Val Val Thr Asp Ser Ala Cys Ala Ser Thr Ala 65 70 75 80 Gly Ser Trp Tyr Ser Pro Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser 85 90 95 Asp Val Asp Ile Asp His Met Val Pro Leu Ser Asn Ala Trp Lys Ser 100 105 110 Gly Ala Ala Gin Trp Thr Thr Ala Arg Arg Gin Asp Phe Ala Asn Asp 115 120 125 Leu Thr Asn Pro Gin Leu Phe Ala Val Thr Asp Asn Val Asn Gin Glu 130 135 140 Lys Gly Asp Lys Gly Pro Glu Asp Trp Lys Pro Ser Leu Thr Ser Tyr 145 150 155 160 Tyr Cys Thr Tyr Ala Lys Ala Trp Val Lys Val Lys Ser Val Trp Ala 165 170 175 Leu Thr Ile Thr Ser Ala Glu Lys Ser Ala Leu Thr Thr Met Leu Asn 180 185 190 Thr Cys <210> 60 <211> 190 <212> PRT <213> Neosartorya massa <4 0 0 > 6 0 Ile Pro Ala Pro Val Ala Leu Pro Thr Pro Pro Gly Ile Pro Ser Ala 15 10 15 Ala Thr Ala Glu Ser Glu Leu Ala Ala Leu Thr Val Ala Ala Gin Gly 20 25 30 Ser Ser Ser Gly Tyr Ser Arg Asp Leu Phe Pro His Trp Ile Ser Gin 35 40 45 Gly Gly Ser Cys Asn Thr Arg Glu Val Val Leu Ala Arg Asp Gly Ser 50 55 60 Gly Val Val Lys Asp Ser Asn Cys Tyr Pro Thr Ser Gly Ser Trp Tyr 65 70 75 80 Ser Pro Tyr Asp Gly Ala Thr Trp Thr Gin Ala Ser Asp Val Asp Ile 85 90 95 Asp His Val Val Pro Leu Ala Asn Ala Trp Arg Ser Gly Ala Ser Lys 100 105 110 Trp Thr Thr Ser Gin Arg Gin Ala Phe Ala Asn Asp Leu Thr Asn Pro 115 120 125 Gin Leu Met Ala Val Thr Asp Asn Val Asn Gin Ala Lys Gly Asp Asp 130 135 140 Gly Pro Glu Ala Trp Lys Pro Pro Leu Thr Ser Tyr Tyr Cys Thr Tyr 1Ί 5 150 155 160 Ala Lys Met Trp Val Arg Val Lys Tyr Val Tyr Asp Leu Thr Ile Thr 165 170 175 Ser Ala Glu Lys Ser Ala Leu Val Ser Met Leu Asp Thr Cys 180 185 190 <210> 61 <211> 191 <212> PRT <213> Roussoella intermedia <4 00> 61 Ala Pro Thr Pro Ala Leu Leu Pro Arg Ala Pro Pro Asn Ile Pro Ser 15 10 15 Thr Ala Thr Ala Lys Ser Gin Leu Ala Ala Leu Thr Val Ala Ala Gin 20 25 30 Gly Pro Gin Asp Gly Tyr Ser Arg Asp Leu Phe Pro His Trp Ile Thr 35 40 45 Gin Ser Gly Ser Cys Asn Thr Arg Glu Val Val Leu Lys Arg Asp Gly 50 55 60 Thr Asn Val Val Gin Asp Ser Ser Cys Ala Ala Thr Ser Gly Thr Trp 65 70 75 80 Val Ser Pro Phe Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val Asp 85 90 95 Ile Asp His Leu Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala Ala 100 105 110 Ser Trp Thr Thr Ala Arg Arg Gin Ser Phe Ala Asn Asp Leu Thr Asn 115 120 125 Pro Gin Leu Leu Ala Val Thr Asp Glu Val Asn Gin Ala Lys Gly Asp 130 135 140 Lys Gly Pro Glu Ala Trp Lys Pro Pro Leu Ala Ser Tyr His Cys Thr 145 150 155 160 Tyr Ala Lys Met Trp Val Lys Val Lys Ser Thr Tyr Ser Leu Thr Ile 165 170 175 Thr Ser Ala Glu Lys Ser Ala Leu Thr Thr Met Leu Asn Thr Cys 180 185 190 <210> 62 <211> 191 <212> PRT <213> Pleosporales <4 00> 62 Leu Pro Thr Pro Ser Leu Val Lys Arg Thr Pro Pro Asn Ile Pro Ser 15 10 15 Thr Thr Ser Ala Lys Ser Leu Leu Ala Gly Leu Thr Val Ala Ala Gin 20 25 30 Gly Pro Gin Asp Gly Tyr Ser Arg Asp Leu Phe Pro His Trp Ile Thr 35 40 45 Ile Ser Gly Thr Cys Asn Thr Arg Glu Thr Val Leu Lys Arg Asp Gly 50 55 60 Thr Asn Val Val Thr Asp Ser Ala Cys Ala Ser Thr Ser Gly Ser Trp 65 70 75 80 Tyr Ser Thr Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val Asp 85 90 95 Ile Asp His Val Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala Ala 100 105 110 Ser Trp Thr Thr Ala Arg Arg Gin Ser Phe Ala Asn Asp Leu Thr Asn H5 120 125 Pro Gin Leu Ile Ala Val Thr Asp Ser Val Asn Gin Ser Lys Gly Asp 130 135 140 Lys Gly Pro Glu Ser Trp Lys Pro Pro Leu Thr Ser Tyr His Cys Thr 145 150 155 160 Tyr Ala Lys Met Trp Val Lys Val Lys Asp Val Tyr Ser Leu Thr Val 165 170 175 Thr Ser Ala Glu Lys Ser Ala Leu Thr Thr Met Leu Asn Thr Cys 180 185 190 <210> 63 <211> 192 <212> PRT <213> Phaeosphaeria sp. <4 00> 63 Leu Pro Ala Pro Ile His Leu Thr Ala Arg Ala Pro Pro Asn Ile Pro 1 5 10 15 Ser Ala Ser Glu Ala Arg Thr Gin Leu Ala Gly Leu Thr Val Ala Ala 20 25 30 Gin Gly Pro Gin Asp Gly Tyr Ser Arg Asp Leu Phe Pro His Trp Ile 35 40 45 Thr Gin Ser Gly Thr Cys Asn Thr Arg Glu Thr Val Leu Lys Arg Asp 50 55 60 Gly Thr Asn Val Val Thr Asn Ser Ala Cys Ala Ser Thr Ser Gly Ser 65 70 75 80 Trp Phe Ser Pro Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val 85 90 95 Asp Ile Asp His Met Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala 100 105 110 Ala Ser Trp Thr Thr Ala Arg Arg Gin Ala Phe Ala Asn Asp Leu Thr 115 120 125 Asn Pro Gin Leu Leu Ala Val Thr Asp Asn Val Asn Gin Ala Lys Gly 130 135 140 Asp Lys Gly Pro Glu Asp Trp Lys Pro Pro Leu Thr Ser Tyr Tyr Cys 145 150 155 160 Thr Tyr Ala Arg Met Trp Val Lys Val Lys Ser Val Tyr Ala Leu Thr 165 170 175 Val Thr Ser Ala Glu Lys Ser Ala Leu Thr Ser Met Leu Gly Thr Cys 180 185 190 <210> 64 <211> 189 <212> PRT <213> Didymosphaeria futilis <4 0 0 > 6 4 Leu Pro Thr Pro Asn Thr Leu Glu Ala Arg Ala Pro Pro Asn Ile Pro 15 10 15 Ser Thr Ser Ala Ala Gin Ser Gin Leu Ser Ala Leu Thr Val Ala Ala 20 25 30 Gin Gly Pro Gin Thr Gly Tyr Ser Arg Asp Leu Phe Pro His Trp Ile 35 40 45 Thr Gin Ser Gly Thr Cys Asn Thr Arg Glu Thr Val Leu Lys Arg Asp 50 55 60 Gly Thr Asn Val Leu Thr Asp Ser Ala Cys Ala Ser Thr Ser Gly Ser 65 70 75 80 Trp Lys Ser Pro Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val 85 90 95 Asp Ile Asp His Val Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala 100 105 110 Ala Ser Trp Thr Thr Ala Arg Arg Gin Ser Phe Ala Asn Asp Leu Thr 115 120 125 Asn Pro Gin Leu Ile Ala Val Thr Asp Asn Val Asn Gin Ala Lys Gly 130 135 140 Asp Lys Gly Pro Glu Asp Trp Lys Pro Pro Leu Thr Ser Tyr Tyr Cys 145 150 155 160 Thr Tyr Ala Lys Met Trp Val Lys Val Lys Ser Val Tyr Ser Leu Thr 165 170 175 Ile Thr Ser Ala Glu Lys Ser Ala Leu Thr Met Leu Ala 180 185 <210> 65 <211> 109 <212> PRT <213> Bacillus licheniformis <4 00> 65 Ala Arg Tyr Asp Asp Ile Leu Tyr Phe Pro Ala Ser Arg Tyr Pro Glu 15 10 15 Thr Gly Ala His Ile Ser Asp Ala Ile Lys Ala Gly His Ser Asp Val 20 25 30 Cys Thr Ile Glu Arg Ser Gly Ala Asp Lys Arg Arg Gin Glu Ser Leu 35 40 45 Lys Gly Ile Pro Thr Lys Pro Gly Phe Asp Arg Asp Glu Trp Pro Met 50 55 60 Ala Met Cys Glu Glu Gly Gly Lys Gly Ala Ser Val Arg Tyr Val Ser 65 70 75 80 Ser Ser Asp Asn Arg Gly Ala Gly Ser Trp Val Gly Asn Arg Leu Ser 85 90 95 Gly Phe Ala Asp Gly Thr Arg He Leu Phe Ile Val Gin 100 105 <210> 66 <211> 110 <212> PRT <213> Bacillus subtilis <4 00> 66 Ala Ser Ser Tyr Asp Lys Val Leu Tyr Phe Pro Leu Ser Arg Tyr Pro 1 5 10 15 Glu Thr Gly Ser His lie Arg Asp Ala lie Ala Glu Gly His Pro Asp 20 25 30 He Cys Thr He Asp Arg Asp Gly Ala Asp Lys Arg Arg Glu Glu Ser 35 40 45 Leu Lys Gly He Pro Thr Lys Pro Gly Tyr Asp Arg Asp Glu Trp Pro 50 55 60 Met Ala Val Cys Glu Glu Gly Gly Ala Gly Ala Asp Val Arg Tyr Val 65 70 75 80 Thr Pro Ser Asp Asn Arg Gly Ala Gly Ser Trp Val Gly Asn Gin Met 85 90 95 Ser Ser Tyr Pro Asp Gly Thr Arg Val Leu Phe He Val Gin 100 105 no <210> 67 <211> 221 <212> PRT <213> Aspergillus oryzae <4 00> 67 Val Pro Val Asn Pro Glu Pro Asp Ala Thr Ser Val Glu Asn Val Ala 1 5 10 15 Leu Lys Thr Gly Ser Gly Asp Ser Gin Ser Asp Pro Ile Lys Ala Asp 20 25 30 Leu Glu Val Lys Gly Gin Ser Ala Leu Pro Phe Asp Val Asp Cys Trp 35 40 45 Ala Ile Leu Cys Lys Gly Ala Pro Asn Val Leu Gin Arg Val Asn Glu 50 55 60 Lys Thr Lys Asn Ser Asn Arg Asp Arg Ser Gly Ala Asn Lys Gly Pro 65 70 75 80 Phe Lys Asp Pro Gin Lys Trp Gly Ile Lys Ala Leu Pro Pro Lys Asn 85 90 95 Pro Ser Trp Ser Ala Gin Asp Phe Lys Ser Pro Glu Glu Tyr Ala Phe 100 105 110 Ala Ser Ser Leu Gin Gly Gly Thr Asn Ala Ile Leu Ala Pro Val Asn 115 120 125 Leu Ala Ser Gin Asn Ser Gin Gly Gly Val Leu Asn Gly Phe Tyr Ser 130 135 140 Ala Asn Lys Val Ala Gin Phe Asp Pro Ser Lys Pro Gin Gin Thr Lys 145 150 155 i g g Gly Thr Trp Phe Gin Ile Thr Lys Phe Thr Gly Ala Ala Gly Pro Tyr 165 170 175 Cys Lys Ala Leu Gly Ser Asn Asp Lys Ser Val Cys Asp Lys Asn Lys 180 105 290 Asn Ile Ala Gly Asp Trp Gly Phe Asp Pro Ala Lys Trp Ala Tyr Gin 195 200 205 Tyr Asp Glu Lys Asn Asn Lys Phe Asn Tyr Val Gly Lys 210 215 220 < 210 > 68 <211> 188 <212> PRT <213> Trichoderma harzianum <400> 68 Ala Pro Ala Pro Met Pro Thr Pro Pro Gly Ile Pro Thr Glu Ser Ser 15 10 15 Ala Arg Thr Gin Leu Ala Gly Leu Thr Val Ala Val Ala Gly Ser Gly 20 25 30 Thr Gly Tyr Ser Arg Asp Leu Phe Pro Thr Trp Asp Ala Ile Ser Gly 35 40 45 Asn Cys Asn Ala Arg Glu Tyr Val Leu Lys Arg Asp Gly Glu Gly Val 50 55 60 Gin Val Asn Asn Ala Cys Glu Ser Gin Ser Gly Thr Trp Ile Ser Pro 65 70 75 80 Tyr Asp Asn Ala Ser Phe Thr Asn Ala Ser Ser Leu Asp Ile Asp His 85 90 95 Met Val Pro Leu Lys Asn Ala Trp Ile Ser Gly Ala Ser Ser Trp Thr 100 105 110 Thr Ala Gin Arg Glu Ala Leu Ala Asn Asp Val Ser Arg Pro Gin Leu 115 120 125 Trp Ala Val Ser Ala Ser Ala Asn Arg Ser Lys Gly Asp Arg Ser Pro 130 135 140 Asp Gin Trp Lys Pro Pro Leu Thr Ser Phe Tyr Cys Thr Tyr Ala Lys 145 150 155 160 Ser Trp Ile Asp Val Lys Ser Phe Tyr Lys Leu Thr Ile Thr Ser Ala 165 170 175 Glu Lys Thr Ala Leu Ser Ser Met Leu Asp Thr Cys 180 185 <210 > 69 <211> 269 <212> PRT <213> bacillus lentus <4 00> 69 Ala Gin Ser Val Pro Trp Gly Ile Ser Arg Val Gin Ala Pro Ala Ala 15 10 15 His Asn Arg Gly Leu Thr Gly Ser Gly Val Lys Val Ala Val Leu Asp 20 25 30 Thr Gly Ile Ser Thr His Pro Asp Leu Asn Ile Arg Gly Gly Ala Ser 35 40 45 Phe Val Pro Gly Glu Pro Ser Thr Gin Asp Gly Asn Gly His Gly Thr 50 55 60 His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu 65 70 75 so Gly Val Ala Pro Ser Ala Glu Leu Tyr Ala Val Lys Val Leu Gly Ala 85 90 95 Ser Gly Ser Gly Ser Val Ser Ser Ile Ala Gin Gly Leu Glu Trp Ala 100 105 110 Gly Asn Asn Gly Met His Val Ala Asn Leu Ser Leu Gly Ser Pro Ser 115 120 125 Pro Ser Ala Thr Leu Glu Gin Ala Val Asn Ser Ala Thr Ser Arg Giv 130 135 140 Val Leu Val Val Ala Ala Ser Gly Asn Ser Gly Ala Gly Ser Ile Ser 145 150 155 i6o Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gin 165 170 175 Asn Asn Asn Arg Ala Ser Phe Ser Gin Tyr Gly Ala Gly Leu Asp Ile 180 185 190 Val Ala Pro Gly Val Asn Val Gin Ser Thr Tyr Pro Gly Ser Thr Tyr 195 200 205 Ala Ser Leu Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ala 210 215 220 Ala Ala Leu Val Lys Gin Lys Asn Pro Ser Trp Ser Asn Val Gin lie 225 230 235 240 Arg Asn His Leu Lys Asn Thr Ala Thr Ser Leu Gly Ser Thr Asn Leu 245 250 255 Tyr Gly Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 260 265 <210> 70 <211> 275 <212> PRT <213> Bacillus amyloliquefaciens <4 00> 70 Ala Gin Ser Val Pro Tyr Gly Val Ser Gin He Lys Ala Pro Ala Leu 1 5 10 15 His Ser Gin Gly Tyr Thr Gly Ser Asn Val Lys Val Ala Val lie Asp 20 25 30 Ser Gly He Asp Ser Ser His Pro Asp Leu Lys Val Ala Gly Gly Ala 35 40 45 Ser Met Val Pro Ser Glu Thr Asn Pro Phe Gin Asp Asn Asn Ser His 50 55 60 Gly Thr His Val Ala Gly Thr Val Ala Ala Leu Asn Asn Ser lie Gly 65 70 75 80 Val Leu Gly Val Ala Pro Ser Ala Ser Leu Tyr Ala Val Lys Val Leu 85 90 95 Gly Ala Asp Gly Ser Gly Gin Tyr Ser Trp He He Asn Gly lie Glu 100 105 110 Trp Ala lie Ala Asn Asn Met Asp Val He Asn Met Ser Leu Gly Gly 115 120 125 Pro Ser Gly Ser Ala Ala Leu Lys Ala Ala Val Asp Lys Ala Val Ala 130 135 140 Ser Gly Val Val Val Val Ala Ala Ala Gly Asn Glu Gly Thr Ser Gly 145 150 155 160 Ser Ser Ser Thr Val Gly Tyr Pro Gly Lys Tyr Pro Ser Val lie Ala 165 170 175 Val Gly Ala Val Asp Ser Ser Asn Gin Arg Ala Ser Phe Ser Ser Val 180 185 190 Gly Pro Glu Leu Asp Val Met Ala Pro Gly Val Ser lie Gin Ser Thr 195 200 205 Leu Pro Gly Asn Lys Tyr Gly Ala Tyr Asn Gly Thr Ser Met Ala Ser 210 215 220 Pro His Val Ala Gly Ala Ala Ala Leu lie Leu Ser Lys His Pro Asn 225 230 235 240 Trp Thr Asn Thr Gin Val Arg Ser Ser Leu Glu Asn Thr Thr Thr Lys 245 250 255 Leu Gly Asp Ser Phe Tyr Tyr Gly Lys Gly Leu lie Asn Val Gin Ala 260 265 270 Ala Ala Gin 275 <210> 71 <211> 311 <212> PRT <213> Bacillus subtilis <4 00> 71 Ala Val Pro Ser Thr Gin Thr Pro Trp Gly lie Lys Ser lie Tyr Asn 15 10 15 Asp Gin Ser lie Thr Lys Thr Thr Gly Gly Ser Gly lie Lys Val Ala 20 25 30 Val Leu Asp Thr Gly Val Tyr Thr Ser His Leu Asp Leu Ala Gly Ser 35 40 45 Ala Glu Gin Cys Lys Asp Phe Thr Gin Ser Asn Pro Leu Val Asp Giv 50 55 60 Ser Cys Thr Asp Arg Gin Gly His Gly Thr His Val Ala Gly Thr Val 65 70 75 80 Leu Ala His Gly Gly Ser Asn Gly Gin Gly Val Tyr Gly Val Ala Pro 90 95 Gin Ala Lys Leu Trp Ala Tyr Lys Val Leu Gly Asp Asn Gly Ser Gly 100 105 110 Tyr Ser Asp Asp Ile Ala Ala Ala Ile Arg His Val Ala Asp Glu Ala 115 120 125 Ser Arg Thr Gly Ser Lys Val Val Ile Asn Met Ser Leu Gly Ser Ser 130 135 i4o Ala Lys Asp Ser Leu Ile Ala Ser Ala Val Asp Tyr Ala Tyr Gly Lys 145 150 155 160 Gly Val Leu Ile Val Ala Ala Ala Gly Asn Ser Gly Ser Gly Ser Asn 165 170 175 Thr Ile Gly Phe Pro Gly Gly Leu Val Asn Ala Val Ala Val Ala Ala 180 185 190 Leu Glu Asn Val Gin Gin Asn Gly Thr Tyr Arg Val Ala Asp Phe Ser 195 200 205 Ser Arg Gly Asn Pro Ala Thr Ala Gly Asp Tyr Ile Ile Gin Glu Arg 210 215 220 Asp Ile Glu Val Ser Ala Pro Gly Ala Ser Val Glu Ser Thr Trp Tyr 225 230 235 240 Thr Gly Gly Tyr Asn Thr Ile Ser Gly Thr Ser Met Ala Thr Pro His 245 250 255 Val Ala Gly Leu Ala Ala Lys Ile Trp Ser Ala Asn Thr Ser Leu Ser 260 265 270 His Ser Gin Leu Arg Thr Glu Leu Gin Asn Arg Ala Lys Val Tyr Asp 275 280 285 Ile Lys Gly Gly Ile Gly Ala Gly Thr Gly Asp Asp Tyr Ala Ser Gly 290 295 300 Phe Gly Tyr Pro Arg Val Lys 305 310 <210> 72 <211> 298 <212> PRT <213> Bacillus bogoriensis <4 00> 72 Ala Asn Ser Gly Phe Tyr Val Ser Gly Thr Thr Leu Tyr Asp Ala Asn 15 10 15 Gly Asn Pro Phe Val Met Arg Gly Ile Asn His Gly His Ala Trp Tyr 20 25 30 Lys Asp Gin Ala Thr Thr Ala Ile Glu Gly Ile Ala Asn Thr Gly Ala 35 40 45 Asn Thr Val Arg Ile Val Leu Ser Asp Gly Gly Gin Trp Thr Lys Asp 50 55 60 Asp Ile His Thr Val Arg Asn Leu Ile Ser Leu Ala Glu Asp Asn His 65 70 75 80 Leu Val Ala Val Leu Glu Val His Asp Ala Thr Gly Tyr Asp Ser Ile 85 90 95 Ala Ser Leu Asn Arg Ala Val Asp Tyr Trp Ile Glu Met Arg Ser Ala 100 105 110 Leu Ile Gly Lys Glu Asp Thr Val Ile Ile Asn Ile Ala Asn Glu Trp 115 120 125 Phe Gly Ser Trp Glu Gly Asp Ala Trp Ala Asp Gly Tyr Lys Gin Ala 130 135 140 lie Pro Arg Leu Arg Asn Ala Gly Leu Asn His Thr Leu Met Val Asp 145 150 155 160 Ala Ala Gly Trp Gly Gin Phe Pro Gin Ser Ile His Asp Tyr Gly Arg 165 170 175 Glu Val Phe Asn Ala Asp Pro Gin Arg Asn Thr Met Phe Ser He His 180 185 190 Met Tyr Glu Tyr Ala Gly Gly Asn Ala Ser Gin Val Arg Thr Asn lie 195 200 205 Asp Arg Val Leu Asn Gin Asp Leu Ala Leu Val He Gly Glu Phe Gly 210 215 220 His Arg His Thr Asn Gly Asp Val Asp Glu Ala Thr He Met Ser Tyr 225 230 235 240 Ser Glu Gin Arg Gly Val Gly Trp Leu Ala Trp Ser Trp Lys Gly Asn 245 250 255 Gly Pro Glu Trp Glu Tyr Leu Asp Leu Ser Asn Asp Trp Ala Gly Asn 260 265 270 Asn Leu Thr Ala Trp Gly Asn Thr He Val Asn Gly Pro Tyr Gly Leu 275 280 285 Arg Glu Thr Ser Arg Leu Ser Thr Val Phe 290 295 <210> 73 <211> 278 <212> PRT <213> thielavia terrestris <4 00> 73 Ala Ser Gly Ser Gly Gin Ser Thr Arg Tyr Trp Asp Cys Cys Lys Pro 1 5 10 15 Ser Cys Ala Trp Pro Gly Lys Ala Ala Val Ser Gin Pro Val Tyr Ala 20 25 30 Cys Asp Ala Asn Phe Gin Arg Leu Ser Asp Phe Asn Val Gin Ser Gly 35 40 45 Cys Asn Gly Gly Ser Ala Tyr Ser Cys Ala Asp Gin Thr Pro Trp Ala 50 55 60 Val Asn Asp Asn Leu Ala Tyr Gly Phe Ala Ala Thr Ser Ile Ala Gly 65 70 75 80 Gly Ser Glu Ser Ser Trp Cys Cys Ala Cys Tyr Ala Leu Thr Phe Thr 85 90 95 Ser Gly Pro Val Ala Gly Lys Thr Met Val Val Gin Ser Thr Ser Thr 100 105 110 Gly Gly Asp Leu Gly Ser Asn Gin Phe Asp Ile Ala Met Pro Gly Gly 120 125 Gly Val Gly Ile Phe Asn Gly Cys Ser Ser Gin Phe Gly Gly Leu Pro 130 135 140 Gly Ala Gin Tyr Gly Gly Ile Ser Ser Arg Asp Gin Cys Asp Ser Phe 145 150 155 160 Pro Ala Pro Leu Lys Pro Gly Cys Gin Trp Arg Phe Asp Trp Phe Gin 165 170 175 Asn Ala Asp Asn Pro Thr Phe Thr Phe Gin Gin Val Gin Cys Pro Ala 180 185 190 Glu Ile Val Ala Arg Ser Gly Cys Lys Arg Asn Asp Asp Ser Ser Phe 195 200 205 Pro Val Phe Thr Pro Pro Ser Gly Gly Asn Gly Gly Thr Gly Thr Pro 21° 215 220 Thr Ser Thr Ala Pro Gly Ser Gly Gin Thr Ser Pro Gly Gly Gly Ser ^ 230 235 240 Gly Cys Thr Ser Gin Lys Trp Ala Gin Cys Gly Gly Ile Gly Phe Ser 245 250 255 GIy Cys Thr Thr Cys Val Ser Gly Thr Thr Cys Gin Lys Leu Asn Asp 260 265 270 Tyr Phe Ser Gin Cys Leu 275 <210> 74 <211> 470 <212> PRT <213> humicola insolens <4 00> 74 Met Arg Ser Ser Pro Leu Leu Arg Ser Ala Val Val Ala Ala Leu Pro 1 5 10 15 Val Leu Ala Leu Ala Ala Asp Gly Arg Ser Thr Arg Tyr Trp Asp Cys 20 25 30 Cys Lys Pro Ser Cys Gly Trp Ala Lys Lys Ala Pro Val Asn Gin Pro 35 40 45 Val Phe Ser Cys Asn Ala Asn Phe Gin Arg Ile Thr Asp Phe Asp Ala 50 55 60 Lys Ser Gly Cys Glu Pro Gly Gly Val Ala Tyr Ser Cys Ala Asp Gin 65 70 75 g0 Thr Pro Trp Ala Val Asn Asp Asp Phe Ala Leu Gly Phe Ala Ala Thr 85 90 95 Ser Ile Ala Gly Ser Asn Glu Ala Gly Trp Cys Cys Ala Cys Tyr Glu 100 105 110 Leu Thr Phe Thr Ser Gly Pro Val Ala Gly Lys Lys Met Val Val Gin 115 120 125 Ser Thr Ser Thr Gly Gly Asp Leu Gly Ser Asn His Phe Asp Leu Asn 150 135 240 Ile Pro Gly Gly Gly Val Gly Ile Phe Asp Gly Cys Thr Pro Gin Phe 145 150 155 160 Gly Gly Leu Pro Gly Gin Arg Tyr Gly Gly Ile Ser Ser Arg Asn Glu !65 170 175 Cys Asp Arg Phe Pro Asp Ala Leu Lys Pro Gly Cys Tyr Trp Arg Phe 180 185 190 Asp Trp Phe Lys Asn Ala Asp Asn Pro Ser Phe Ser Phe Arg Gin Val 195 200 205 Gin Cys Pro Ala Glu Leu Val Ala Arg Thr Gly Cys Arg Arg Asn Asp 210 215 220 Asp Gly Asn Phe Pro Ala Val Gin Ile Pro Ser Ser Ser Thr Ser Ser 225 230 235 240 Pro Val Asn Gin Pro Thr Ser Thr Ser Thr Thr Ser Thr Ser Thr Thr 245 250 255 Ser Ser Pro Pro Val Gin Pro Thr Thr Pro Ser Gly Cys Ala Asp Gly 260 265 270 Arg Ser Thr Arg Tyr Trp Asp Cys Cys Lys Pro Ser Cys Gly Trp Ala 275 280 285 Lys Lys Ala Pro Val Asn Gin Pro Val Phe Ser Cys Asn Ala Asn Phe 290 295 300 Gin Arg Ile Thr Asp Phe Asp Ala Lys Ser Gly Cys Glu Pro Gly Gly 305 310 315 320 Val Ala Tyr Ser Cys Ala Asp Gin Thr Pro Trp Ala Val Asn Asp Asp 325 330 335 Phe Ala Leu Gly Phe Ala Ala Thr Ser Ile Ala Gly Ser Asn Glu Ala 340 345 350 Gly Trp Cys Cys Ala Cys Tyr Glu Leu Thr Phe Thr Ser Gly Pro Val 355 360 365 Ala Gly Lys Lys Met Val Val Gin Ser Thr Ser Thr Gly Gly Asp Leu 37° 375 380 Gly Ser Asn His Phe Asp Leu Asn Ile Pro Gly Gly Gly Val Gly Ile 385 390 395 400 Phe Asp Gly Cys Thr Pro Gin Phe Gly Gly Leu Pro Gly Gin Arg Tyr 405 410 415 Gly Gly Ile Ser Ser Arg Asn Glu Cys Asp Arg Phe Pro Asp Ala Leu 420 425 430 Lys Pro Gly Cys Tyr Trp Arg Phe Asp Trp Phe Lys Asn Ala Asp Asn 435 440 445 Pro Ser Phe Ser Phe Arg Gin Val Gin Cys Pro Ala Glu Leu Val Ala 450 455 460 Arg Thr Gly Cys Arg Arg 465 470 <210> 75 <211> 425 <212> PRT <213> Escherichia coli <4 00> 75 Thr Ala Leu Leu Leu Gly Leu Val Asn Gly Gin Lys Pro Gly Glu Thr 15 10 15 Lys Glu Val His Pro Gin Leu Thr Thr Phe Arg Cys Thr Lys Arg Gly 20 25 30 Gly Cys Lys Pro Ala Thr Asn Phe Ile Val Leu Asp Ser Leu Ser His 35 40 45 Pro Ile His Arg Ala Glu Gly Leu Gly Pro Gly Gly Cys Gly Asp Trp 50 55 60 Gly Asn Pro Pro Pro Lys Asp Val Cys Pro Asp Val Glu Ser Cys Ala 65 70 75 80 Lys Asn Cys Ile Met Glu Gly Ile Pro Asp Tyr Ser Gin Tyr Gly Val 85 90 95 Thr Thr Asn Gly Thr Ser Leu Arg Leu Gin His Ile Leu Pro Asp Gly 100 105 110 Arg Val Pro Ser Pro Arg Val Tyr Leu Leu Asp Lys Thr Lys Arg Arg 115 120 125 Tyr Glu Met Leu His Leu Thr Gly Phe Glu Phe Thr Phe Asp Val Asp 130 135 140 Ala Thr Lys Leu Pro Cys Gly Met Asn Ser Ala Leu Tyr Leu Ser Glu 145 150 155 160 Met His Pro Thr Gly Ala Lys Ser Lys Tyr Asn Pro Gly Gly Ala Tyr 165 170 175 Tyr Gly Thr Gly Tyr Cys Asp Ala Gin Cys Phe Val Thr Pro Phe Ile 180 185 190 Asn Gly Leu Gly Asn Ile Glu Gly Lys Gly Ser Cys Cys Asn Glu Met !95 200 205 Asp Ile Trp Glu Ala Asn Ser Arg Ala Ser His Val Ala Pro His Thr 210 215 220 Cys Asn Lys Lys Gly Leu Tyr Leu Cys Glu Gly Glu Glu Cys Ala Phe 225 230 235 240 Glu Gly Val Cys Asp Lys Asn Gly Cys Gly Trp Asn Asn Tyr Arg Val 245 250 255 Asn Val Thr Asp Tyr Tyr Gly Arg Gly Glu Glu Phe Lys Val Asn Thr 2^0 265 270 Leu Lys Pro Phe Thr Val Val Thr Gin Phe Leu Ala Asn Arg Arg Gly 275 280 285 Lys Leu Glu Lys Ile His Arg Phe Tyr Val Gin Asp Gly Lys Val Ile 290 295 300 Glu Ser Phe Tyr Thr Asn Lys Glu Gly Val Pro Tyr Thr Asn Met Ile 305 Ήη 315 320 Asp Asp Glu Phe Cys Glu Ala Thr Gly Ser Arg Lys Tyr Met Glu Leu 325 330 335 Gly Ala Thr Gin Gly Met Gly Glu Ala Leu Thr Arg Gly Met Val Leu 340 345 350 Ala Met Ser He Trp Trp Asp Gin Gly Gly Asn Met Glu Trp Leu Asp 355 360 365 His Gly Glu Ala Gly Pro Cys Ala Lys Gly Glu Gly Ala Pro Ser Asn 370 375 380 lie Val Gin Val Glu Pro Phe Pro Glu Val Thr Tyr Thr Asn Leu Arg 385 390 395 400 Trp Gly Glu He Gly Ser Thr Tyr Gin Glu Val Gin Lys Pro Lys Pro 405 410 415 Lys Pro Gly His Gly Pro Arg Ser Asp 420 425 <210> 76 <211> 773 <212> PRT <213> bacillus subtilis <4 00> 76 Ala Glu Gly Asn Thr Arg Glu Asp Asn Phe Lys His Leu Leu Gly Asn 1 5 10 15 Asp Asn Val Lys Arg Pro Ser Glu Ala Gly Ala Leu Gin Leu Gin Glu 20 25 30 Val Asp Gly Gin Met Thr Leu Val Asp Gin His Gly Glu Lys He Gin 35 40 45 Leu Arg Gly Met Ser Thr His Gly Leu Gin Trp Phe Pro Glu He Leu 50 55 60 Asn Asp Asn Ala Tyr Lys Ala Leu Ala Asn Asp Trp Glu Ser Asn Met 65 70 75 80 Ile Arg Leu Ala Met Tyr Val Gly Glu Asn Gly Tyr Ala Ser Asn Pro 85 90 95 Glu Leu Ile Lys Ser Arg Val Ile Lys Gly Ile Asp Leu Ala Ile Glu 100 105 110 Asn Asp Met Tyr Val Ile Val Asp Trp His Val His Ala Pro Gly Asp 115 120 125 Pro Arg Asp Pro Val Tyr Ala Gly Ala Glu Asp Phe Phe Arg Asp Ile 130 135 140 Ala Ala Leu Tyr Pro Asn Asn Pro His Ile Ile Tyr Glu Leu Ala Asn 145 150 155 160 Glu Pro Ser Ser Asn Asn Asn Gly Gly Ala Gly Ile Pro Asn Asn Glu 165 170 175 Glu Gly Trp Asn Ala Val Lys Glu Tyr Ala Asp Pro Ile Val Glu Met 180 185 190 Leu Arg Asp Ser Gly Asn Ala Asp Asp Asn Ile Ile Ile Val Gly Ser 195 200 205 Pro Asn Trp Ser Gin Arg Pro Asp Leu Ala Ala Asp Asn Pro Ile Asn 210 215 220 Asp His His Thr Met Tyr Thr Val His Phe Tyr Thr Gly Ser His Ala 225 230 235 240 Ala Ser Thr Glu Ser Tyr Pro Pro Glu Thr Pro Asn Ser Glu Arg Gly 245 250 255 Asn Val Met Ser Asn Thr Arg Tyr Ala Leu Glu Asn Gly Val Ala Val 260 265 270 Phe Ala Thr Glu Trp Gly Thr Ser Gin Ala Asn Gly Asp Gly Gly Pro 275 280 285 Tyr Phe Asp Glu Ala Asp Val Trp Ile Glu Phe Leu Asn Glu Asn Asn 290 295 300 Ile Ser Trp Ala Asn Trp Ser Leu Thr Asn Lys Asn Glu Val Ser Gly 305 310 315 320 Ala Phe Thr Pro Phe Glu Leu Gly Lys Ser Asn Ala Thr Asn Leu Asp 325 330 335 Pro Gly Pro Asp His Val Trp Ala Pro Glu Glu Leu Ser Leu Ser Gly 340 345 350 Glu Tyr Val Arg Ala Arg Ile Lys Gly Val Asn Tyr Glu Pro Ile Asp 355 360 365 Arg Thr Lys Tyr Thr Lys Val Leu Trp Asp Phe Asn Asp Gly Thr Lys 37° 375 380 Gin Gly Phe Gly Val Asn Ser Asp Ser Pro Asn Lys Glu Leu Ile Ala 385 390 395 400 Val Asp Asn Glu Asn Asn Thr Leu Lys Val Ser Gly Leu Asp Val Ser 405 410 415 Asn Asp Val Ser Asp Gly Asn Phe Trp Ala Asn Ala Arg Leu Ser Ala 420 425 430 Asp Gly Trp Gly Lys Ser Val Asp Ile Leu Gly Ala Glu Lys Leu Thr 435 440 445 Met Asp Val Ile Val Asp Glu Pro Thr Thr Val Ala Ile Ala Ala Ile 450 455 460 Pro Gin Ser Ser Lys Ser Gly Trp Ala Asn Pro Glu Arg Ala Val Arg 465 470 475 480 Val Asn Ala Glu Asp Phe Val Gin Gin Thr Asp Gly Lys Tyr Lys Ala 485 490 495 Gly Leu Thr Ile Thr Gly Glu Asp Ala Pro Asn Leu Lys Asn Ile Ala 500 505 510 Phe His Glu Glu Asp Asn Asn Met Asn Asn Ile Ile Leu Phe Val Gly 515 520 525 Thr Asp Ala Ala Asp Val Ile Tyr Leu Asp Asn Ile Lys Val Ile Gly 530 535 540 Thr Glu Val Glu Ile Pro Val Val His Asp Pro Lys Gly Glu Ala Val 545 550 555 560 Leu Pro Ser Val Phe Glu Asp Gly Thr Arg Gin Gly Trp Asp Trp Ala 565 570 575 Gly Glu Ser Gly Val Lys Thr Ala Leu Thr Ile Glu Glu Ala Asn Gly 580 585 590 Ser Asn Ala Leu Ser Trp Glu Phe Gly Tyr Pro Glu Val Lys Pro Ser 595 600 605 Asp Asn Trp Ala Thr Ala Pro Arg Leu Asp Phe Trp Lys Ser Asp Leu 610 615 620 Vål Arg Gly Glu Asn Asp Tyr Val Ala Phe Asp Phe Tyr Leu Asp Pro 625 630 635 640 Val Arg Ala Thr Glu Gly Ala Met Asn Ile Asn Leu Val Phe Gin Pro 645 650 655 Pro Thr Asn Gly Tyr Trp Val Gin Ala Pro Lys Thr Tyr Thr Ile Asn 660 665 670 Phe Asp Glu Leu Glu Glu Ala Asn Gin Val Asn Gly Leu Tyr His Tyr 675 680 685 Glu Val Lys Ile Asn Val Arg Asp Ile Thr Asn Ile Gin Asp Asp Thr 690 695 700 Leu Leu Arg Asn Met Met Ile Ile Phe Ala Asp Val Glu Ser Asp Phe 705 710 715 720 Ala Gly Arg Val Phe Val Asp Asn Val Arg Phe Glu Gly Ala Ala Thr 725 730 735 Thr Glu Pro Val Glu Pro Glu Pro Val Asp Pro Gly Glu Glu Thr Pro 740 745 750 Pro Val Asp Glu Lys Glu Ala Lys Lys Glu Gin Lys Glu Ala Glu Lys 755 760 765 Glu Glu Lys Glu Glu 770 <210> 77 <211> 524 <212> PRT <213> Paenibacillus polymyxa <400> 77 Val Val His Gly Gin Thr Ala Lys Thr Ile Thr Ile Lys Val Asp Thr 1 5 10 15 Phe Lys Asp Arg Lys Pro Ile Ser Pro Tyr Ile Tyr Gly Thr Asn Gin 20 25 30 Asp Leu Ala Gly Asp Glu Asn Met Ala Ala Arg Arg Leu Gly Gly Asn 35 40 45 Arg Met Thr Gly Tyr Asn Trp Glu Asn Asn Met Ser Asn Ala Gly Ser 50 55 60 Asp Trp Gin His Ser Ser Asp Asn Tyr Leu Cys Ser Asn Gly Gly Leu 65 70 75 80 Thr Gin Ala Glu Cys Glu Lys Pro Gly Ala Val Val Thr Ser Phe His 85 90 95 Asp Gin Ser Leu Lys Leu Gly Thr Tyr Ser Leu Val Thr Leu Pro Met 100 105 110 Ala Gly Tyr Val Ala Ala Asp Gly Asn Gly Ser Val Gin Glu Ser Glu 115 120 125 Ala Ala Pro Ser Ala Arg Trp Asn Gin Val Val Asn Ala Lys Asn Ala 130 135 140 Pro Phe Gin Leu Gin Pro Asp Leu Asn Asp Asn Tyr Val Tyr Val Asp I45 150 155 160 Glu Phe Val His Phe Leu Val Asn Lys Tyr Gly Thr Ala Ser Thr Lys 165 170 175 Ala Gly Val Lys Gly Tyr Ala Leu Asp Asn Glu Pro Ala Leu Trp Ser 180 185 190 His Thr His Pro Arg He His Pro Glu Lys Val Gly Ala Lys Glu Leu 195 200 205 Val Asp Arg Ser Val Ser Leu Ser Lys Ala Val Lys Ala lie Asp Ala 210 215 220 Gly Ala Glu Val Phe Gly Pro Val Leu Tyr Gly Phe Gly Ala Tyr Lys 225 230 235 240 Asp Leu Gin Thr Ala Pro Asp Trp Asp Ser Val Lys Gly Asn Tyr Ser 245 250 255 Trp Phe Val Asp Tyr Tyr Leu Asp Gin Met Arg Leu Ser Ser Gin Val 260 265 270 Glu Gly Lys Arg Leu Leu Asp Val Phe Asp Val His Trp Tyr Pro Glu 275 280 285 Ala Met Gly Gly Gly He Arg He Thr Asn Glu Val Gly Asn Asp Glu 290 295 300 Thr Lys Lys Ala Arg Met Gin Ala Pro Arg Thr Leu Trp Asp Pro Thr 305 310 315 320 Tyr Lys Glu Asp Ser Trp lie Ala Gin Trp Phe Ser Glu Phe Leu Pro 325 330 335 He Leu Pro Arg Leu Lys Gin Ser Val Asp Lys Tyr Tyr Pro Gly Thr 340 345 350 Lys Leu Ala Met Thr Glu Tyr Ser Tyr Gly Gly Glu Asn Asp He Ser 355 360 365 Gly Gly He Ala Met Thr Asp Val Leu Gly lie Leu Gly Lys Asn Asp 370 375 380 Val Tyr Met Ala Asn Tyr Trp Lys Leu Lys Asp Gly Val Asn Asn Tyr 385 390 395 400 Val Ser Ala Ala Tyr Lys Leu Tyr Arg Asn Tyr Asp Gly Lys Asn Ser 405 410 415 Thr Phe Gly Asp Thr Ser Val Ser Ala Gin Thr Ser Asp Ile Val Asn 420 425 430 Ser Ser Val His Ala Ser Val Thr Asn Ala Ser Asp Lys Glu Leu His 435 440 445 Leu Val Val Met Asn Lys Ser Met Asp Ser Ala Phe Asp Ala Gin Phe 450 455 460 Asp Leu Ser Gly Ala Lys Thr Tyr Ile Ser Gly Lys Val Trp Gly Phe 465 470 475 480 Asp Lys Asn Ser Ser Gin Ile Lys Glu Ala Ala Pro Ile Thr Gin Ile 485 490 495 Ser Gly Asn Arg Phe Thr Tyr Thr Val Pro Pro Leu Thr Ala Tyr His 500 505 510 Ile Val Leu Thr Thr Gly Asn Asp Thr Ser Pro Val 515 520 <210> 78 <211> 485 <212> PRT <213> Bacillus sp. <4 00> 78 His His Asn Gly Thr Asn Gly Thr Met Met Gin Tyr Phe Glu Trp Tyr 1 5 10 15 Leu Pro Asn Asp Gly Asn His Trp Asn Arg Leu Arg Ser Asp Ala Ser 20 25 30 Asn Leu Lys Asp Lys Gly Ile Ser Ala Val Trp Ile Pro Pro Ala Trp 35 40 45 Lys Gly Ala Ser Gin Asn Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr 50 55 60 Asp Leu Gly Glu Phe Asn Gin Lys Gly Thr Ile Arg Thr Lys Tyr Gly 65 70 75 80 Thr Arg Asn Gin Leu Gin Ala Ala Val Asn Ala Leu Lys Ser Asn Gly 85 90 95 lie Gin Val Tyr Gly Asp Val Val Met Asn His Lys Gly Gly Ala Asp 100 105 HO Ala Thr Glu Met Val Arg Ala Val Glu Val Asn Pro Asn Asn Arg Asn 115 120 125 Gin Glu Val Ser Gly Glu Tyr Thr He Glu Ala Trp Thr Lys Phe Asp 130 135 140 Phe Pro Gly Arg Gly Asn Thr His Ser Asn Phe Lys Trp Arg Trp Tyr 145 150 155 160 His Phe Asp Gly Val Asp Trp Asp Gin Ser Arg Lys Leu Asn Asn Arg 105 170 175 He Tyr Lys Phe Arg Gly Asp Gly Lys Gly Trp Asp Trp Glu Val Asp 180 185 190 Thr Glu Asn Gly Asn Tyr Asp Tyr Leu Met Tyr Ala Asp He Asp Met 195 200 205 Asp His Pro Glu Val Val Asn Glu Leu Arg Asn Trp Gly Val Trp Tyr 210 215 220 Thr Asn Thr Leu Gly Leu Asp Gly Phe Arg He Asp Ala Val Lys His 225 230 235 240 He Lys Tyr Ser Phe Thr Arg Asp Trp He Asn His Val Arg Ser Ala 245 250 255 Thr Gly Lys Asn Met Phe Ala Val Ala Glu Phe Trp Lys Asn Asp Leu 260 265 270 Gly Ala He Glu Asn Tyr Leu Asn Lys Thr Asn Trp Asn His Ser Val 275 280 285 Phe Asp Val Pro Leu His Tyr Asn Leu Tyr Asn Ala Ser Lys Ser Gly 290 295 300 Gly Asn Tyr Asp Met Arg Gin Ile Phe Asn Gly Thr Val Val Gin Arg 305 310 315 320 His Pro Met His Ala Val Thr Phe Val Asp Asn His Asp Ser Gin Pro 325 330 335 Glu Glu Ala Leu Glu Ser Phe Val Glu Glu Trp Phe Lys Pro Leu Ala 340 345 350 Tyr Ala Leu Thr Leu Thr Arg Glu Gin Gly Tyr Pro Ser Val Phe Tyr 355 360 365 Gly Asp Tyr Tyr Gly He Pro Thr His Gly Val Pro Ala Met Lys Ser 370 375 380 Lys lie Asp Pro lie Leu Glu Ala Arg Gin Lys Tyr Ala Tyr Gly Arg 335 390 395 400 Gin Asn Asp Tyr Leu Asp His His Asn He He Gly Trp Thr Arg Glu 405 410 415 Gly Asn Thr Ala His Pro Asn Ser Gly Leu Ala Thr He Met Ser Asp 420 425 430 Gly Ala Gly Gly Asn Lys Trp Met Phe Val Gly Arg Asn Lys Ala Gly 435 440 445 Gin Val Trp Thr Asp He Thr Gly Asn Arg Ala Gly Thr Val Thr He 450 455 460 Asn Ala Asp Gly Trp Gly Asn Phe Ser Val Asn Gly Gly Ser Val Ser 465 47° 475 480 He Trp Val Asn Lys 485 <210> 79 <211> 485 <212> PRT <213> Bacillus sp. NCIB 12513 <400> 79 His His Asn Gly Thr Asn Gly Thr Met Met Gin Tyr Phe Glu Trp His 1 5 10 15 Leu Pro Asn Asp Gly Asn His Trp Asn Arg Leu Arg Asp Asp Ala Ser 20 25 30 Asn Leu Arg Asn Arg Gly Ile Thr Ala Ile Trp Ile Pro Pro Ala Trp 35 40 45 Lys Gly Thr Ser Gin Asn Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr 50 55 60 Asp Leu Gly Glu Phe Asn Gin Lys Gly Thr Val Arg Thr Lys Tyr Gly 65 70 75 80 Thr Arg Ser Gin Leu Glu Ser Ala Ile His Ala Leu Lys Asn Asn Gly 85 90 95 Val Gin Val Tyr Gly Asp Val Val Met Asn His Lys Gly Gly Ala Asp 100 105 110 Ala Thr Glu Asn Val Leu Ala Val Glu Val Asn Pro Asn Asn Arg Asn H5 120 125 Gin Glu Ile Ser Gly Asp Tyr Thr Ile Glu Ala Trp Thr Lys Phe Asp 130 135 140 Phe Pro Gly Arg Gly Asn Thr Tyr Ser Asp Phe Lys Trp Arg Trp Tyr I45 150 155 160 His Phe Asp Gly Val Asp Trp Asp Gin Ser Arg Gin Phe Gin Asn Arg 165 170 175 Ile Tyr Lys Phe Arg Gly Asp Gly Lys Ala Trp Asp Trp Glu Val Asp 180 185 190 Ser Glu Asn Gly Asn Tyr Asp Tyr Leu Met Tyr Ala Asp Val Asp Met 195 200 205 Asp His Pro Glu Val Val Asn Glu Leu Arg Arg Trp Gly Glu Trp Tyr 210 215 220 Thr Asn Thr Leu Asn Leu Asp Gly Phe Arg Ile Asp Ala Val Lys His 225 230 235 240 Ile Lys Tyr Ser Phe Thr Arg Asp Trp Leu Thr His Val Arg Asn Ala 245 250 255 Thr Gly Lys Glu Met Phe Ala Val Ala Glu Phe Trp Lys Asn Asp Leu 260 265 270 Gly Ala Leu Glu Asn Tyr Leu Asn Lys Thr Asn Trp Asn His Ser Val 275 280 285 Phe Asp Val Pro Leu His Tyr Asn Leu Tyr Asn Ala Ser Asn Ser Gly 290 295 300 Gly Asn Tyr Asp Met Ala Lys Leu Leu Asn Gly Thr Val Val Gin Lys 305 310 315 320 His Pro Met His Ala Val Thr Phe Val Asp Asn His Asp Ser Gin Pro 325 330 335 Gly Glu Ser Leu Glu Ser Phe Val Gin Glu Trp Phe Lys Pro Leu Ala 340 345 350 Tyr Ala Leu Ile Leu Thr Arg Glu Gin Gly Tyr Pro Ser Val Phe Tyr 355 360 365 Gly Asp Tyr Tyr Gly Ile Pro Thr His Ser Val Pro Ala Met Lys Ala 370 375 380 Lys Ile Asp Pro Ile Leu Glu Ala Arg Gin Asn Phe Ala Tyr Gly Thr 385 390 395 400 Gin His Asp Tyr Phe Asp His His Asn Ile Ile Gly Trp Thr Arg Glu 405 410 415 Gly Asn Thr Thr His Pro Asn Ser Gly Leu Ala Thr Ile Met Ser Asp 420 425 430 Gly Pro Gly Gly Glu Lys Trp Met Tyr Val Gly Gin Asn Lys Ala Gly 435 440 445 Gin Val Trp His Asp Ile Thr Gly Asn Lys Pro Gly Thr Val Thr Ile 450 455 460 Asn Ala Asp Gly Trp Ala Asn Phe Ser Val Asn Gly Gly Ser Val Ser 465 470 475 480 Ile Trp Val Lys Arg 485 <210> 80 <211> 485 <212> PRT <213> Bacillus sp. no. 707 <4 00> 80 His His Asn Gly Thr Asn Gly Thr Met Met Gin Tyr Phe Glu Trp Tyr 1 5 10 15 Leu Pro Asn Asp Gly Asn His Trp Asn Arg Leu Asn Ser Asp Ala Ser 20 25 30 Asn Leu Lys Ser Lys Gly Ile Thr Ala Val Trp Ile Pro Pro Ala Trp 35 40 45 Lys Gly Ala Ser Gin Asn Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr 50 55 60 Asp Leu Gly Glu Phe Asn Gin Lys Gly Thr Val Arg Thr Lys Tyr Gly 65 70 75 80 Thr Arg Ser Gin Leu Gin Ala Ala Val Thr Ser Leu Lys Asn Asn Gly 85 90 95 Ile Gin Val Tyr Gly Asp Val Val Met Asn His Lys Gly Gly Ala Asp !00 105 110 Ala Thr Glu Met Val Arg Ala Val Glu Val Asn Pro Asn Asn Arg Asn H5 120 125 Gin Glu Val Thr Gly Glu Tyr Thr Ile Glu Ala Trp Thr Arg Phe Asp 130 135 140 Phe Pro Gly Arg Gly Asn Thr His Ser Ser Phe Lys Trp Arg Trp Tyr 145 150 155 160 His Phe Asp Gly Val Asp Trp Asp Gin Ser Arg Arg Leu Asn Asn Arg 165 170 175 Ile Tyr Lys Phe Arg Gly His Gly Lys Ala Trp Asp Trp Glu Val Asp 180 185 190 Thr Glu Asn Gly Asn Tyr Asp Tyr Leu Met Tyr Ala Asp Ile Asp Met 195 200 205 Asp His Pro Glu Val Val Asn Glu Leu Arg Asn Trp Gly Val Trp Tyr 210 215 220 Thr Asn Thr Leu Gly Leu Asp Gly Phe Arg Ile Asp Ala Val Lys His 225 230 235 240 Ile Lys Tyr Ser Phe Thr Arg Asp Trp Ile Asn His Val Arg Ser Ala 245 250 255 Thr Gly Lys Asn Met Phe Ala Val Ala Glu Phe Trp Lys Asn Asp Leu 260 265 270 Gly Ala Ile Glu Asn Tyr Leu Gin Lys Thr Asn Trp Asn His Ser Val 275 280 285 Phe Asp Val Pro Leu His Tyr Asn Leu Tyr Asn Ala Ser Lys Ser Gly 290 295 300 Gly Asn Tyr Asp Met Arg Asn Ile Phe Asn Gly Thr Val Val Gin Arg 305 310 315 320 His Pro Ser His Ala Val Thr Phe Val Asp Asn His Asp Ser Gin Pro 325 330 335 Glu Glu Ala Leu Glu Ser Phe Val Glu Glu Trp Phe Lys Pro Leu Ala 340 345 350 Tyr Ala Leu Thr Leu Thr Arg Glu Gin Gly Tyr Pro Ser Val Phe Tyr 355 360 365 Gly Asp Tyr Tyr Gly He Pro Thr His Gly Val Pro Ala Met Arg Ser 370 375 3go Lys lie Asp Pro He Leu Glu Ala Arg Gin Lys Tyr Ala Tyr Gly Lys 385 390 395 400 Gin Asn Asp Tyr Leu Asp His His Asn He Leu Gly Trp Thr Arg Glu 405 410 415 Gly Asn Thr Ala His Pro Asn Ser Gly Leu Ala Thr He Met Ser Asp 420 425 430 Gly Ala Gly Gly Ser Lys Trp Met Phe Val Gly Arg Asn Lys Ala Gly 435 440 445 Gin Val Trp Ser Asp He Thr Gly Asn Arg Thr Gly Thr Val Thr He 450 455 460 Asn Ala Asp Gly Trp Gly Asn Phe Ser Val Asn Gly Gly Ser Val Ser 465 470 475 480 He Trp Val Asn Lys 485 <210> 81 <211> 485 <212> PRT <213> artificial <220> <223> Protein <400> 81 His His Asp Gly Thr Asn Gly Thr He Met Gin Tyr Phe Glu Trp Asn 1 5 io 15 Val Pro Asn Asp Gly Gin His Trp Asn Arg Leu His Asn Asn Ala Gin 20 25 30 Asn Leu Lys Asn Ala Gly He Thr Ala He Trp He Pro Pro Ala Trp 35 40 45 Lys Gly Thr Ser Gin Asn Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr 50 55 gø Asp Leu Gly Glu Phe Asn Gin Lys Gly Thr Val Arg Thr Lys Tyr Gly 65 70 75 80 Thr Lys Ala Glu Leu Glu Arg Ala Ile Arg Ser Leu Lys Ala Asn Gly 85 90 95 Ile Gin Val Tyr Gly Asp Val Val Met Asn His Lys Gly Gly Ala Asp 100 105 110 Phe Thr Glu Arg Val Gin Ala Val Glu Val Asn Pro Gin Asn Arg Asn H5 120 125 Gin Glu Val Ser Gly Thr Tyr Gin Ile Glu Ala Trp Thr Gly Phe Asn 130 135 140 Phe Pro Gly Arg Gly Asn Gin His Ser Ser Phe Lys Trp Arg Trp Tyr 145 150 155 160 His Phe Asp Gly Thr Asp Trp Asp Gin Ser Arg Gin Leu Ala Asn Arg 165 170 175 Ile Tyr Lys Phe Arg Gly Asp Gly Lys Ala Trp Asp Trp Glu Val Asp 180 185 190 Thr Glu Asn Gly Asn Tyr Asp Tyr Leu Met Tyr Ala Asp Val Asp Met 195 200 205 Asp His Pro Glu Val Ile Asn Glu Leu Asn Arg Trp Gly Val Trp Tyr 210 215 220 Ala Asn Thr Leu Asn Leu Asp Gly Phe Arg Leu Asp Ala Val Lys His 225 230 235 240 Ile Lys Phe Ser Phe Met Arg Asp Trp Leu Gly His Val Arg Gly Gin 245 250 255 Thr Gly Lys Asn Leu Phe Ala Val Ala Glu Tyr Trp Lys Asn Asp Leu 260 265 270 Gly Ala Leu Glu Asn Tyr Leu Ser Lys Thr Asn Trp Thr Met Ser Ala 275 280 285 Phe Asp Val Pro Leu His Tyr Asn Leu Tyr Gin Ala Ser Asn Ser Ser 290 295 300 Gly Asn Tyr Asp Met Arg Asn Leu Leu Asn Gly Thr Leu Val Gin Arg 305 310 315 320 His Pro Ser His Ala Val Thr Phe Val Asp Asn His Asp Thr Gin Pro 325 330 335 Gly Glu Ala Leu Glu Ser Phe Val Gin Gly Trp Phe Lys Pro Leu Ala 340 345 350 Tyr Ala Thr Ile Leu Thr Arg Glu Gin Gly Tyr Pro Gin Val Phe Tyr 355 360 365 Gly Asp Tyr Tyr Gly Ile Pro Ser Asp Gly Val Pro Ser Tyr Arg Gin 370 375 380 Gin Ile Asp Pro Leu Leu Lys Ala Arg Gin Gin Tyr Ala Tyr Gly Thr 385 390 395 400 Gin His Asp Tyr Leu Asp Asn Gin Asp Val Ile Gly Trp Thr Arg Glu 405 410 415 Gly Asp Ser Ala His Ala Gly Ser Gly Leu Ala Thr Val Met Ser Asp 420 425 430 Gly Pro Gly Gly Ser Lys Thr Met Tyr Val Gly Thr Ala His Ala Gly 435 440 445 Gin Val Phe Lys Asp Ile Thr Gly Asn Arg Thr Asp Thr Val Thr Ile 450 455 460 Asn Ser Ala Gly Asn Gly Thr Phe Pro Cys Asn Gly Gly Ser Val Ser 465 470 475 430 Ile Trp Val Lys Gin 485 <210> 82 <211> 600 <212> PRT <213> Curvularia verruculosa <4 00> 82 Met Gly Ser Val Thr Pro Ile Pro Leu Pro Thr Ile Asp Glu Pro Glu 15 10 15 Glu Tyr Asn Asn Asn Tyr Ile Leu Phe Trp Asn Asn Val Gly Leu Glu 20 25 30 Leu Asn Arg Leu Thr His Thr Val Gly Gly Pro Leu Thr Gly Pro Pro 35 40 45 Leu Ser Ala Arg Ala Leu Gly Met Leu His Leu Ala Ile His Asp Ala 50 55 60 Tyr Phe Ser Ile Cys Pro Pro Thr Glu Phe Thr Thr Phe Leu Ser Pro 65 70 75 80 Asp Ala Glu Asn Pro Ala Tyr Arg Leu Pro Ser Pro Asn Gly Ala Asp 85 90 95 Asp Ala Arg Gin Ala Val Ala Gly Ala Ala Leu Lys Met Leu Ser Ser 100 105 110 Leu Tyr Met Lys Pro Ala Asp Pro Asn Thr Gly Thr Asn Ile Ser Asp 115 120 125 Asn Ala Tyr Ala Gin Leu Ala Leu Val Leu Glu Arg Ala Val Val Lys 130 135 140 Val Pro Gly Gly Val Asp Arg Glu Ser Val Ser Phe Met Phe Gly Glu 145 150 155 160 Ala Val Ala Asp Val Phe Phe Ala Leu Leu Asn Asp Pro Arg Gly Ala 165 170 175 Ser Gin Glu Gly Tyr Gin Pro Thr Pro Gly Arg Tyr Lys Phe Asp Asp 180 185 190 Glu Pro Thr His Pro Val Val Leu Val Pro Val Asp Pro Asn Asn Pro 1.95 200 205 Asn Gly Pro Lys Met Pro Phe Arg Gin Tyr His Ala Pro Phe Tyr Gly 210 215 220 Met Thr Thr Lys Arg Phe Ala Thr Gin Ser Glu His Ile Leu Ala Asp 225 230 235 240 Pro Pro Gly Leu Arg Ser Asn Ala Asp Glu Thr Ala Glu Tyr Asp Asp 245 250 255 Ser Ile Arg Val Ala Ile Ala Met Gly Gly Ala Gin Asp Leu Asn Ser 260 265 270 Thr Lys Arg Ser Pro Trp Gin Thr Ala Gin Gly Leu Tyr Trp Ala Tyr 275 280 285 Asp Gly Ser Asn Leu Val Gly Thr Pro Pro Arg Phe Tyr Asn Gin Ile 290 295 300 Val Arg Arg Ile Ala Val Thr Tyr Lys Lys Glu Asp Asp Leu Ala Asn 305 310 315 320 Ser Glu Val Asn Asn Ala Asp Phe Ala Arg Leu Phe Ala Leu Val Asn 325 330 335 Val Ala Cys Thr Asp Ala Gly Ile Phe Ser Trp Lys Glu Lys Trp Glu 340 345 350 Phe Glu Phe Trp Arg Pro Leu Ser Gly Val Arg Asp Asp Gly Arg Pro 355 360 365 Asp His Gly Asp Pro Phe Trp Leu Thr Leu Gly Ala Pro Ala Thr Asn 370 375 380 Thr Asn Asp Ile Pro Phe Lys Pro Pro Phe Pro Ala Tyr Pro Ser Gly 385 390 395 400 His Ala Thr Phe Gly Gly Ala Val Phe Gin Met Val Arg Arg Tyr Tyr 405 410 415 Asn Gly Arg Val Gly Thr Trp Lys Asp Asp Glu Pro Asp Asn Ile Ala 420 425 430 Ile Asp Met Met Ile Ser Glu Glu Leu Asn Gly Val Asn Arg Asp Leu 435 440 445 Arg Gin Pro Tyr Asp Pro Thr Ala Pro Ile Glu Asp Gin Pro Gly Ile 450 455 460 Val Arg Thr Arg Ile Val Arg His Phe Asp Ser Ala Trp Glu Met Met 465 470 475 480 Phe Glu Asn Ala Ile Ser Arg Ile Phe Leu Gly Val His Trp Arg Phe 485 490 495 Asp Ala Ala Ala Ala Arg Asp Ile Leu Ile Pro Thr Asn Thr Lys Asp 500 505 510 Val Tyr Ala Val Asp Ser Asn Gly Ala Thr Val Phe Gin Asn Val Glu 515 520 525 Asp Val Arg Tyr Ser Thr Lys Gly Thr Arg Glu Gly Arg Glu Gly Leu 530 535 540 Phe Pro Ile Gly Gly Val Pro Leu Gly Ile Glu Ile Ala Asp Glu Ile 545 550 555 560 Phe Asn Asn Gly Leu Arg Pro Thr Pro Pro Glu Leu Gin Pro Met Pro 565 570 575 Gin Asp Thr Pro Val Gin Lys Pro Val Gin Gly Met Trp Asp Glu Gin 580 585 590 Val Pro Leu Val Lys Glu Ala Pro 595 600 <210> 83 <211> 485 <212> PRT <213> Artificial <220> <223> Amylase enzyme < 4 O O > 83 His His Asp Gly Thr Asn Gly Thr Ile Met Gin Tyr Phe Glu Trp Asn 15 10 15 Val Pro Asn Asp Gly Gin His Trp Asn Arg Leu His Asn Asn Ala Gin 20 25 30 Asn Leu Lys Asn Ala Gly Ile Thr Ala Ile Trp Ile Pro Pro Ala Trp 35 40 45 Lys Gly Thr Ser Gin Asn Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr 50 55 60 Asp Leu Gly Glu Phe Asn Gin Lys Gly Thr Val Arg Thr Lys Tyr Gly 65 70 75 80 Thr Lys Ala Glu Leu Glu Arg Ala Ile Arg Ser Leu Lys Ala Asn Gly 85 90 95 Ile Gin Val Tyr Gly Asp Val Val Met Asn His Lys Gly Gly Ala Asp 105 110 Phe Thr Glu Arg Val Gin Ala Val Glu Val Asn Pro Gin Asn Arg Asn H5 120 125 Gin Glu Val Ser Gly Thr Tyr Gin Ile Glu Ala Trp Thr Gly Phe Asn 130 135 140 Phe Pro Gly Arg Gly Asn Gin His Ser Ser Phe Lys Trp Arg Trp Tyr 145 150 155 160 His Phe Asp Gly Thr Asp Trp Asp Gin Ser Arg Gin Leu Ala Asn Arg 165 170 175 Ile Tyr Lys Phe Arg Gly Asp Gly Lys Ala Trp Asp Trp Glu Val Asp 180 185 190 Thr Glu Asn Gly Asn Tyr Asp Tyr Leu Met Tyr Ala Asp Val Asp Met 195 200 205 Asp His Pro Glu Val Ile Asn Glu Leu Asn Arg Trp Gly Val Trp Tyr 210 215 220 Ala Asn Thr Leu Asn Leu Asp Gly Phe Arg Leu Asp Ala Val Lys His 225 230 235 240 Ile Lys Phe Ser Phe Met Arg Asp Trp Leu Gly His Val Arg Gly Gin 245 250 255 Thr Gly Lys Asn Leu Phe Ala Val Ala Glu Tyr Trp Lys Asn Asp Leu 260 265 270 Gly Ala Leu Glu Asn Tyr Leu Ser Lys Thr Asn Trp Thr Met Ser Ala 275 280 285 Phe Asp Val Pro Leu His Tyr Asn Leu Tyr Gin Ala Ser Asn Ser Ser 290 295 300 Gly Asn Tyr Asp Met Arg Asn Leu Leu Asn Gly Thr Leu Val Gin Arg 305 310 315 320 His Pro Ser His Ala Val Thr Phe Val Asp Asn His Asp Thr Gin Pro 325 330 335 Gly Glu Ala Leu Glu Ser Phe Val Gin Gly Trp Phe Lys Pro Leu Ala 340 345 350 Tyr Ala Thr lie Leu Thr Arg Glu Gin Gly Tyr Pro Gin Val Phe Tyr 355 360 365 Gly Asp Tyr Tyr Gly lie Pro Ser Asp Gly Val Pro Ser Tyr Arg Gin 370 375 380 Gin lie Asp Pro Leu Leu Lys Ala Arg Gin Gin Tyr Ala Tyr Gly Arg 385 390 395 400 Gin His Asp Tyr Phe Asp His Trp Asp Val He Gly Trp Thr Arg Glu 405 410 415 Gly Asn Ala Ser His Pro Asn Ser Gly Leu Ala Thr lie Met Ser Asp 420 425 430 Gly Pro Gly Gly Ser Lys Trp Met Tyr Val Gly Arg Gin Lys Ala Gly 435 440 445 Glu Val Trp His Asp Met Thr Gly Asn Arg Ser Gly Thr Val Thr Ile 450 455 460 Asn Gin Asp Gly Trp Gly His Phe Phe Val Asn Gly Gly Ser Val Ser 465 470 475 480 Val Trp Val Lys Arg 485 <210> 84 <211> 485 <212> PRT <213> Artificial <220> <223> Amylase protein <400> 84 His His Asp Gly Thr Asn Gly Thr Ile Met Gin Tyr Phe Glu Trp Asn 1 5 10 15 Val Pro Asn Asp Gly Gin His Trp Asn Arg Leu His Asn Asn Ala Gin 20 25 30 Asn Leu Lys Asn Ala Gly Ile Thr Ala Ile Trp Ile Pro Pro Ala Trp 35 40 45 Lys Gly Thr Ser Gin Asn Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr 50 55 60 Asp Leu Gly Glu Phe Asn Gin Lys Gly Thr Val Arg Thr Lys Tyr Gly 65 70 75 80 Thr Lys Ala Glu Leu Glu Arg Ala Ile Arg Ser Leu Lys Ala Asn Gly 85 90 95 Ile Gin Val Tyr Gly Asp Val Val Met Asn His Lys Gly Gly Ala Asp 100 105 110 Phe Thr Glu Arg Val Gin Ala Val Glu Val Asn Pro Gin Asn Arg Asn 115 120 125 Gin Glu Val Ser Gly Thr Tyr Gin Ile Glu Ala Trp Thr Gly Phe Asn 130 135 140 Phe Pro Gly Arg Gly Asn Gin His Ser Ser Phe Lys Trp Arg Trp Tyr 145 150 155 160 His Phe Asp Gly Thr Asp Trp Asp Gin Ser Arg Gin Leu Ala Asn Arg 165 170 175 Ile Tyr Lys Phe Arg Gly Asp Gly Lys Ala Trp Asp Trp Glu Val Asp 180 185 190 Thr Glu Asn Gly Asn Tyr Asp Tyr Leu Met Tyr Ala Asp Val Asp Met 195 200 205 Asp His Pro Glu Val Ile Asn Glu Leu Asn Arg Trp Gly Val Trp Tyr 210 215 220 Ala Asn Thr Leu Asn Leu Asp Gly Phe Arg Leu Asp Ala Val Lys His 225 230 235 240 Ile Lys Phe Ser Phe Met Arg Asp Trp Leu Gly His Val Arg Gly Gin 245 250 255 Thr Gly Lys Asn Leu Phe Ala Val Ala Glu Tyr Trp Lys Asn Asp Leu 260 265 270 Gly Ala Leu Glu Asn Tyr Leu Ser Lys Thr Asn Trp Thr Met Ser Ala 275 280 285 Phe Asp Val Pro Leu His Tyr Asn Leu Tyr Gin Ala Ser Asn Ser Ser 290 295 300 Gly Asn Tyr Asp Met Arg Asn Leu Leu Asn Gly Thr Leu Val Gin Arg 305 310 315 320 His Pro Ser His Ala Val Thr Phe Val Asp Asn His Asp Thr Gin Pro 325 330 335 Gly Glu Ala Leu Glu Ser Phe Val Gin Gly Trp Phe Lys Pro Leu Ala 340 345 350 Tyr Ala Thr Ile Leu Thr Arg Glu Gin Gly Tyr Pro Gin Val Phe Tyr 355 360 365 Gly Asp Tyr Tyr Gly Ile Pro Ser Asp Gly Val Pro Ser Tyr Arg Gin 370 375 380 Gin Ile Asp Pro Leu Leu Lys Ala Arg Gin Gin Tyr Ala Tyr Gly Thr 385 390 395 400 Gin His Asp Tyr Leu Asp Asn Gin Asp Val Ile Gly Trp Thr Arg Glu 405 410 415 Gly Asp Ser Ala His Ala Gly Ser Gly Leu Ala Thr Val Met Ser Asp 420 425 430 Gly Pro Gly Gly Ser Lys Thr Met Tyr Val Gly Thr Ala His Ala Gly 435 440 445 Gin Val Phe Lys Asp Ile Thr Gly Asn Arg Thr Asp Thr Val Thr Ile 450 455 460 Asn Ser Ala Gly Asn Gly Thr Phe Pro Cys Asn Gly Gly Ser Val Ser 465 470 475 480 Ile Trp Val Lys Gin 485HNA15-2 <4 00> 47 Val Pro Val Pro Ala Pro Pro Gly He Pro Thr Ala Ser Thr Ala Arg 1 5 10 15 Thr Leu Leu Ala Gly Leu Lys Val Ala Thr Pro Leu Ser Gly Asp Gly 20 25 30 Tyr Ser Arg Thr Leu Phe Pro Thr Trp Glu Thr lie Glu Gly Thr Cys 35 40 45 Asn Ala Arg Glu Phe Val Leu Lys Arg Asp Gly Thr Asp Val Gin Thr 50 55 60 Asn Thr Ala Cys Val Ala Gin Ser Gly Asn Trp Val Ser Pro Tyr Asp 65 70 75 80 Gly Val Ala Phe Thr Ala Ser Asp Leu Asp He Asp His With Val 85 90 95 Pro Leu Lys Asn Ala Trp Ile Ser Gly Ala Ser Gin Trp Thr Thr Asp 100 105 110 Lys Arg List Gly Leu Ala Asn Asp Ile Thr Arg Pro Gin Leu Trp Ala 115 120 125 Val Ser Ala His Ala Asn Arg Ala Lys Gly Asp Ser Ser Pro Asp Glu 130 135 140 Trp Lys Pro Pro Leu Lys Thr Phe Trp Cys Thr Tyr Ala Arg Ser Trp 145 150 155 160 Val Gin Val Lys Ser Tyr Tyr Ala Leu Thr lie Thr Asp Ala Glu Lys 165 170 175 Gly Ala Leu Ser Gly With Leu Asp Ser Cys 180 185 <210> 48 <211> 188 <212> PRT <213> Acremonium sp. XZ2414 <4 00> 48 Ala Pro lie Ala Val Arg Asp Pro Gly lie Pro Ser Ala Ser Thr 15 10 15 Ala Asn Thr Leu Leu Ala Gly Leu Thr Val Arg Ala Ser Ser Asn Glu 20 25 30 Asp Ser Tyr Asp Arg Asn Leu Phe Pro His Trp Ser Ala He Ser Gly 35 40 45 Asn Cys Asn Ala Arg Glu Phe Val Leu Glu Arg Asp Gly Thr Asn Val 50 55 60 Val Val Asn Asn Ala Cys Val Ala Gin Ser Gly Thr Trp Arg Ser Pro 65 70 75 80 Tyr Asp Gly Glu Thr Thr Gly Asn Ala Ser Asp Leu Asp lie Asp His 85 90 95 With Val Pro Leu Lys Asn Ala Trp Ile Ser Gly Ala Ser Ser Trp Ser 100 105 no Thr Thr Arg Arg Gin Glu Phe Ala Asn Asp Val Ser Gly Pro Gin Leu 115 120 125 Trp Ala Val Thr Ala Gly Val Asn Arg Ser Lys Gly Asp Arg Ser Pro 130 135 140 Asp Ser Trp Val Pro Pro Leu Ala Ser Phe His Cys Thr Tyr Ala Lys 145 150 155 160 Ser Trp Val Gin Val Lys Ser Ser Trp Ser Leu Ser Val Thr Ser Ala 165 170 175 Glu Lys Ala Ala Leu Ser Asp Leu Leu Gly Thr Cys 180 185 <210> 49 <211> 186 <212> PRT <213> Isaria tenuipes <4 00> 49 Ala Pro Val Pro Glu Pro Pro Gly lie Pro Ser Thr Ser Thr Ala Gin 15 10 15 Ser Asp Leu Asn Ser Leu Gin Val Ala Ala Ser Gly Ser Gly Asp Gly 20 25 30 Tyr Ser Arg Ala Glu Phe Pro His Trp Val Ser Val Glu Gly Ser Cys 35 40 45 Asp Ser Arg Glu Tyr Val Leu Lys Arg Asp Gly Gin Asp Val Gin Ala 50 55 60 Asp Ser Ser Cys Lys He Thr Ser Gly Thr Trp Val Ser Pro Tyr Asp 65 70 75 80 Ala Thr Thr Trp Thr Asn Ser Ser Lys Val Asp Asp His Leu Val 85 90 95 Pro Leu Lys Asn Ala Trp lie Ser Gly Ala Ser Ser Trp Thr Lys Ala 100 105 110 Gin Arg Gin Asp Phe Ala Asn Asp Ile Lys Arg Pro Gin Leu Tyr Ala 115 120 125 Val Ser Glu Asn Ala Asn Arg Ser Lys Gly Asp Arg Ser Pro Asp Gly 130 135 140 Trp List Pro Pro Leu Lys Ser Phe Tyr Cys Thr Tyr Ala Lys Ser Trp 145 150 155 160 Val Ala Val Lys Ser Tyr Tyr Lys Leu Thr He Thr Ser Ala Glu Lys 165 170 175 Ser Ala Leu Gly Asp With Leu Asp Thr Cys 180 185 <210> 50 <211> 184 <212> PRT <213> Scytalidium circinatum <4 00> 50 Ala Pro Pro Gly lie Pro Ser Ala Ser Thr Ala Ser Ser Leu Leu Gly 1 5 10 15 Glu Leu Ala Val Ala Glu Pro Val Asp Asp Gly Ser Tyr Asp Arg Aso 20 25 30 Leu Phe Pro His Trp Glu Pro He Pro Gly Glu Thr Ala Cys Ser Ala 35 40 45 Arg Glu Tyr Val Leu Arg Arg Asp Gly Thr Gly Val Glu Thr Gly Ser 50 55 60 Asp Cys Tyr Pro Thr Ser Gly Thr Trp Ser Ser Pro Tyr Asp Gly Gly 65 70 75 80 Ser Trp Thr Ala Pro Ser Asp Val Asp Lie Asp His With Val Pro Leu 85 90 95 List Asn Ala Trp He Ser Gly Ala Ser Glu Trp Thr Thr Ala Glu Arq 100 105 440 Glu Ala Phe Ala Asn Asp He Asp Gly Pro Gin Leu Trp Ala Val Thr 115 120 125 Asp Glu Val Asn Gin Ser Lys Ser Asp Gin Ser Pro Asp Glu Trp Lys 130 135 140 Pro Pro Leu Ser Ser Phe Tyr Cys Thr Tyr Ala Cys Ala Trp lie Gin 145 150 155 160 Val Lys Ser Thr Tyr Ser Leu Ser He Ser Ser Ala Glu Gin Ala Ala 165 170 175 Leu Glu Asp With Leu Gly Ser Cys 180 <210> 51 <211> 186 <212> PRT <213> Metarhizium lepidiotae <4 00> 51 Val Pro Val Pro Ala Pro Pro Gly lie Pro Thr Ala Ser Thr Ala Arg 1 5 10 15 Thr Leu Leu Ala Gly Leu Lys Val Ala Thr Pro Leu Ser Gly Asp Gly 20 25 30 Tyr Ser Arg Thr Leu Phe Pro Thr Trp Glu Thr He Glu Gly Thr Cys 35 40 45 Asn Ala Arg Glu Phe Val Leu Lys Arg Asp Gly Thr Asp Val Gin Thr 50 55 60 Asn Thr Ala Cys Val Ala Glu Ser Gly Asn Trp Val Ser Pro Tyr Asp 65 70 75 80 Gly Val Ser Phe Thr Ala Ala Ser Asp Leu Asp He Asp His With Val 85 90 95 Pro Leu Lys Asn Ala Trp He Ser Gly Ala Ser Gin Trp Thr Thr Asp 100 105 110 Lys Arg List Asp Leu Ala Asn Asp Ile Thr Arg Pro Gin Leu Trp Ala 115 120 125 Val Ser Ala His Ala Asn Arg Ser Lys Gly Asp Ser Ser Pro Asp Glu 130 135 140 Trp List Pro Pro Leu Gin Thr Phe Trp Cys Thr Tyr Ser Lys Ser Trp 145 150 155 160 Ile Gin Val Lys Ser His Tyr Ser Leu Thr Ile Thr Asp Ala Glu Lys 165 170 175 Gly Ala Leu Ser Gly With Leu Asp Ser Cys 180 185 <210> 52 <211> 226 <212> PRT <213> Thermobispora bispora <4 00> 52 Leu Asp Ile Ala Asp Gly Arg Pro Ala Gly Gly List Ala Ala Glu Ala 1 5 10 15 Ala Thr Gly Thr Ser Pro Leu Ala Asn Pro Asp Gly Thr Arg Pro Gly 20 25 30 Leu Ala Ala Ile Thr Ser Ala Asp Glu Arg Ala Glu Ala Arg Ala Leu 35 40 45 Ile Glu Arg Leu Arg Thr Lys Gly Arg Gly Pro Lys Thr Gly Tyr Glu 50 55 60 Arg Glu Lys Phe Gly Tyr Ala Trp Ala Asp Ser Val Asp Gly Ile Pro 70 75 80 Phe Gly Arg Asn Gly Cys Asp Thr Arg Asn Asp Val Leu List Arg Asp 85 90 95 Gly Gin Arg Leu Gin Phe Arg Ser Gly Ser Asp Cys Val Val Ile Ser 100 105 110 With Thr Leu Phe Asp Pro Tyr Thr Gly List Thr Ile Glu Trp Thr Lys 115 120 125 Gin Asn Ala Ala Glu Val Gin Ile Asp His Val Val Pro Leu Ser Tyr 130 135 140 Ser Trp Gin With Gly Ala Ser Arg Trp Ser Asp Glu List Arg Arg Gin 145 150 155 160 Leu Ala Asn Asp Pro Leu Asn Leu With Pro Val Asp Gly Ala Thr Asn 165 170 175 Ser Arg List Gly Asp Ser Gly Pro Ala Ser Trp Leu Pro Pro Arg Arg 180 185 190 Glu He Arg Cys Ala Tyr Val Val Arg Phe Ala Gin Val Ala Leu List 195 200 205 Tyr Asp Leu Pro Val Thr Thr Ala Asp List Glu Thr Met Leu Gin Gin 210 215 220 Cys Ser 225 <210> 53 <211> 191 <212> PRT <213> Sporormia fimetaria <4 00> 53 Leu Pro Ala Pro Val Leu Glu Lys Arg Thr Pro Pro Asn He Pro Ser 15 10 15 Thr Ser Thr Ala Gin Ser Leu Leu Ser Gly Leu Thr Val Ala Pro Gin 20 25 30 Gly Ser Gin Thr Gly Tyr Ser Arg Asp Leu Phe Pro His Trp He Thr 35 40 45 Val Ser Gly Thr Cys Asn Thr Arg Glu Thr Val Leu Lys Arg Asp Gly 50 55 60 Ser Asn Val Val Thr Asp Ser Ala Cys Ala Ser Val Ser Gly Ser Trp 65 70 75 80 Tyr Ser Thr Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val Asp 85 90 95 Ile Asp His Val Pro Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala Ala 100 105 110 Ser Trp Thr Thr Ala Arg Arg Gin Ala Phe Ala Asn Asp Leu Thr Asn 115 120 125 Pro Gin Leu Ile Ala Val Thr Asp Asn Val Asn Gin Ala Lys Gly Asp 130 135 140 Gin Gly Pro Glu Ser Trp Lys Pro Pro Leu Thr Ser Tyr Cys Thr 145 150 155 160 Tyr Ala Lys Met Trp Val Lys Val Lys Ser Val Tyr Ser Leu Thr Val 165 170 175 Thr Ser Ala Glu Lys Ser Ala Leu Ser Ser With Leu Gly Thr Cys 180 185 190 <210> 54 <211> 193 <212> PRT <213> Pycnidiophora cf.dispera <4 00> 54 Leu Pro Ala Pro Ala Pro Val Leu Val Ala Arg Glu Pro Pro Asn Ile 1 5 10 15 Pro Ser Thr Ser Ser Ala Gin Ser With Leu Ser Gly Leu Thr Val List 20 25 30 Ala Gin Gly Pro Gin Asp Gly Tyr Ser Arg Asp Leu Phe Pro His Trp 35 40 45 Ile Thr Ile Ser Gly Thr Cys Asn Thr Arg Glu Thr Val Leu Lys Arg 50 55 60 Asp Gly Thr Asn Val Val Thr Asn Ser Ala Cys Ala Ser Thr Ser Gly 65 70 75 80 Ser Trp Tyr Ser Pro Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp 85 90 95 Val Asp Ile Asp His Ile Val Pro Leu Ser Asn Ala Trp Lys Ser Gly 100 105 110 Ala Ala Ser Trp Thr Thr Ser Arg Arg Gin Gin Phe Ala Asn Asp Leu 115 120 125 Thr Asn Pro Gin Leu Ile Ala Val Thr Asp Ser Val Asn Gin Ala Lys 130 135 140 Gly Asp Lys Gly Pro Glu Asp Trp Lys Pro Ser Arg Thr Ser Tyr His 145 150 155 160 Cys Thr Tyr Ala Lys With Trp Ile Lys Val Lys Ser Val Tyr Ser Leu 165 170 175 Thr Val Thr Ser Ala Glu Lys Ser Ala Leu Thr Thr With Leu Asn Thr 180 185 190 Cys <210> 55 <211> 199 <212> PRT <213> Enviromental sample D <4 00> 55 Asp Thr Asp Pro Glu Pro Val Ala Gly Ser Ala Leu Glu Ala Leu Ala 15 10 15 Gly Leu Glu Val List Gly Pro Gly Pro Asp Thr Gly Tyr Glu Arg Ala 20 25 30 Leu Phe Gly Pro Pro Trp Ala Asp Val Asp Gly Asn Gly Cys Asp Thr 35 40 45 Arg Asn Asp Ile Leu Ala Arg Asp Leu Thr Asp Leu Thr Phe Ser Thr 50 55 60 Arg Gly Asp Val Cys Glu Val Arg Thr Gly Thr Phe Asp Pro Tyr 65 70 75 80 Thr Gly Glu Thr Ile Asp Phe Arg Arg Gly Asn Ala Thr Ser Ala Ala 85 90 95 Val Gin Ile Asp His Val Val Pro Leu Leu Asp Ala Trp Arg List Gly 100 105 110 Ala Arg Ala Trp Asp Asp Glu Thr Arg Arg Gin Phe Ala Asn Asp Pro 115 120 125 Leu Asn Leu Leu Ala Ser Asp Gly Pro Ala Asn Gin Ser List Gly Ala 130 135 140 Arg Asp Ala Ser Ala Trp Leu Pro Pro Asn His Ala Phe Arg Cys Pro 145 150 155 160 Tyr Val Ala Arg Gin Ile Ala Val List Ala Ala Tyr Glu Leu Ser Val 165 170 175 Thr Pro Ser Glu Ser Glu Ala With Ala Arg Val Leu Ala Asp Cys Pro 180 185 190 Ala Glu Pro Leu Pro Ala Gly 195 <210> 56 <211> 199 <212> PRT <213> Enviromental sample O <4 00> 56 Asp Asp Glu Pro Glu Pro Ala Arg Gly Ser Ala Leu Glu Ala Leu Ala 1 5 10 15 Arg Leu Glu Val Val Gly Pro Gly Pro Asp Thr Gly Tyr Glu Arg Glu 20 25 30 Leu Phe Gly Pro Ala Trp Ala Asp Val Asp Gly Asn Gly Cys Asp Thr 35 40 45 Arg Asn Asp Ile Leu Ala Asp Asp Leu Thr Asp Leu Thr Phe Ser Thr 50 55 60 Arg Gly Glu Val Cys Glu Val Arg Thr Gly Thr Phe Gin Asp Pro Tyr 65 70 75 80 Thr Gly Glu Thr Ile Asp Phe Arg Arg Gly Asn Ala Thr Ser With Ala 85 90 95 Val Gin Ile Asp His Val Val Pro Leu With Asp Ala Trp Arg List Gly 100 105 110 Ala Arg Ala Trp Asp Asp Glu Thr Arg Arg Gin Phe Ala Asn Asp Pro 115 120 125 Leu Asn Leu Leu Ala Ser Asp Gly Pro Ala Asn Gin Ser List Gly Ala 130 135 140 Arg Asp Ala Ser Ala Trp Leu Pro Pro Asn His Ala Phe Arg Cys Pro 145 150 155 160 Tyr Val Ala Arg Gin Ile Ala Val List Thr Ala Tyr Glu Leu Ser Val 165 170 175 Thr Pro Ser Glu Ser Glu Ala With Ala Arg Val Leu Glu Asp Cys Pro 180 185 190 Ala Glu Pro Val Pro Ala Gly 195 <210> 57 <211> 186 <212> PRT <213> Clavicipitaceae sp-70249 <4 00> 57 Val Pro Val Pro Ala Pro Pro Gly ile Pro Ser Thr Ser Thr Ala Lys 1 5 10 15 Thr Leu Leu Ala Gly Leu Lys Val Ala Thr Pro Leu Ser Gly Asp Gly 20 25 30 Tyr Ser Arg Asp Lys Phe Pro Thr Trp Glu Thr Ile Gin Gly Thr Cys 35 40 45 Asn Ala Arg Glu Phe Val Ile Lys Arg Asp Gly Thr Asp Val Lys Thr 50 55 60 Asn Ser Ala Cys Val Ala Glu Ser Gly Asn Trp Val Ser Pro Tyr Asp 65 70 75 80 Gly Fall List Phe Thr Ala Ala Arg Asp Leu Asp Ile Asp His With Val 85 90 95 Pro Leu Lys Asn Ala Trp Ile Ser Gly Ala Ser Gin Trp Thr Thr Glu 100 105 110 Gin Arg List Ala Leu Ala Asn Asp Ile Thr Arg Pro Gin Leu Trp Ala 115 120 125 Val Ser Ala His Ala Asn Arg Gly Lys Ser Asp Asp Ser Pro Asp Glu 130 135 140 Trp List Pro Pro Leu Lys Thr Phe Trp Cys Thr Tyr Ala Lys Ser Trp 145 150 155 160 Val Gin Val Lys Ser Phe Tyr Lys Leu Thr Ile Thr Asp Thr Glu Lys 165 170 175 Gly Ala Leu Ala Gly With Leu Asp Thr Cys 180 185 <210> 58 <211> 187 <212> PRT <213> Westerdykella sp. AS85-2 <400> 58 Phe Pro Ala Pro Ala Ser Val Leu Glu Ala Arg Ala Pro Pro Asn Ile 15 10 15 Pro Ser Ala Ser Thr Ala Gin Ser Leu Val Gly Leu Thr Val Gin 20 25 30 Pro Gin Gly Pro Gin Asp Gly Tyr Ser Arg Asp Leu Phe Pro His Trp 35 40 45 Ile Thr Ile Ser Gly Thr Cys Asn Thr Arg Glu Thr Val Leu Lys Arg 50 55 60 Asp Gly Ser Asn Val Val Thr Asn Ser Ala Cys Ala Ala Thr Ser Gly 65 70 75 80 Thr Trp Tyr Ser Pro Tyr Asp Gly Ala Thr Trp Thr Ser Ala Ser Asp 85 90 95 Val Asp Ile Asp His Leu Val Pro Leu Ser Asn Ala Trp Lys Ser Gly 100 105 110 Ala Ala Ser Trp Thr Thr Ala Lys Arg Gin Gin Phe Ala Asn Asp Leu 115 120 125 Thr Asn Pro Gin Leu Leu Ala Val Thr Asp Arg Val Asn Gin Ala Lys 130 135 140 Gly Asp Lys Gly Pro Glu Ala Trp Lys Pro Ser Leu Ala Ser Tyr His l45 150 155 160 Cys Thr Tyr Ala List With Trp Val List Val List Ser List Asp Val Arg 165 170 175 Leu Thr Gly Asn Trp Thr List Asp Gly Trp 180 185 <210> 59 <211> 194 <212> PRT <213> Humicolopsis cephalosporioides <4 00> 59 Ala Pro Thr Pro Ala Pro Val Glu Leu Glu Arg Arg Thr Pro Pro Asn 15 10 15 Ile Pro Thr Thr Ala Ser Ala Lys Ser Leu Leu Ala Gly Leu Thr Val 20 25 30 Ala Ala Gin Gly Pro Gin Thr Gly Tyr Ser Arg Asp Leu Phe Pro His 35 40 45 Trp Ile Thr Ile Ser Gly Ser Cys Asn Thr Arg Glu Thr Val Leu Lys 50 55 60 Arg Asp Gly Thr Gly Val Val Thr Asp Ser Ala Cys Ala Ser Thr Ala 65 70 75 80 Gly Ser Trp Tyr Ser Pro Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser 85 90 95 Asp Val Asp Ile Asp His Met Val Pro Leu Ser Asn Ala Trp List Ser 100 105 110 Gly Ala Ala Gin Trp Thr Thr Ala Arg Arg Gin Asp Phe Ala Asn Asp 115 120 125 Leu Thr Asn Pro Gin Leu Phe Ala Val Thr Asp Asn Val Asn Gin Glu 130 135 140 Lys Gly Asp Lys Gly Pro Glu Asp Trp Lys Pro Ser Leu Thr Ser Tyr 145 150 155 160 Tyr Cys Thr Tyr Ala Lys Ala Trp Val Lys Val Lys Ser Val Trp Ala 165 170 175 Leu Thr Ile Thr Ser Ala Glu Lys Ser Ala Leu Thr Thr With Leu Asn 180 185 190 Thr Cys <210> 60 <211> 190 <212> PRT <213> Neosartorya mass <4 0 0> 6 0 Ile Pro Ala Pro Val Ala Leu Pro Thr Pro Pro Gly Ile Pro Ser Ala 15 10 15 Ala Thr Ala Glu Ser Glu Leu Ala Ala Leu Thr Val Ala Ala Gin Gly 20 25 30 Ser Ser Ser Gly Tyr Ser Arg Asp Leu Phe Pro His Trp Ile Ser Gin 35 40 45 Gly Gly Ser Cys Asn Thr Arg Glu Val Val Leu Ala Arg Asp Gly Ser 50 55 60 Gly Val Val Lys Asp Ser Asn Cys Tyr Pro Thr Ser Gly Ser Trp Tyr 65 70 75 80 Ser Pro Tyr Asp Gly Ala Thr Trp Thr Gin Ala Ser Asp Val Asp Ile 85 90 95 Asp His Val Pro Leu Ala Asn Ala Trp Arg Ser Gly Ala Ser Lys 100 105 110 Trp Thr Thr Ser Gin Arg Gin Ala Phe Ala Asn Asp Leu Thr Asn Pro 115 120 125 Gin Leu With Ala Val Thr Asp Asn Val Asn Gin Ala Lys Gly Asp Asp 130 135 140 Gly Pro Glu Ala Trp Lys Pro Pro Leu Thr Ser Tyr Tyr Cys Thr Tyr 1Ί 5 150 155 160 Ala Lys With Trp Val Arg Val Lys Tyr Val Tyr Asp Ile Thr 165 170 175 Ser Ala Glu Lys Ser Ala Leu Val Ser With Leu Asp Thr Cys 180 185 190 <210> 61 <211> 191 <212> PRT <213> Roussoella intermedia <4 00> 61 Ala Pro Thr Pro Ala Leu Pro Arg Ala Pro Pro Asn Ile Pro Ser 15 10 15 Thr Ala Thr Ala Lys Ser Gin Leu Ala Alu Leu Thr Val Ala Ala Gin 20 25 30 Gly Pro Gin Asp Gly Tyr Ser Arg Asp Leu Phe Pro His Trp Ile Thr 35 40 45 Gin Ser Gly Ser Cys Asn Thr Arg Glu Val Val Leu Lys Arg Asp Gly 50 55 60 Thr Asn Val Val Gin Asp Ser Ser Cys Ala Ala Thr Ser Gly Thr Trp 65 70 75 80 Val Ser Pro Phe Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val Asp 85 90 95 Ile Asp His Leu Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala Ala 100 105 110 Ser Trp Thr Thr Ala Arg Arg Gin Ser Phe Ala Asn Asp Leu Thr Asn 115 120 125 Pro Gin Leu Ala Val Thr Asp Glu Val Asn Gin Ala Lys Gly Asp 130 135 140 Lys Gly Pro Glu Ala Trp Lys Pro Pro Leu Ala Ser Tyr His Cys Thr 145 150 155 160 Tyr Ala Lys Met Trp Val Lys Val Lys Ser Thr Tyr Ser Leu Thr Ile 165 170 175 Thr Ser Ala Glu Lys Ser Ala Leu Thr Thr With Leu Asn Thr Cys 180 185 190 <210> 62 <211> 191 <212> PRT <213> Pleosporales <4 00> 62 Leu Pro Thr Pro Ser Leu Val Lys Arg Thr Pro Asn Ile Pro Ser 15 10 15 Thr Thr Ser Ala Lys Ser Leu Leu Ala Gly Leu Thr Val Ala Ala Gin 20 25 30 Gly Pro Gin Asp Gly Tyr Ser Arg Asp Leu Phe Pro His Trp Ile Thr 35 40 45 Ile Ser Gly Thr Cys Asn Thr Arg Glu Thr Val Leu Lys Arg Asp Gly 50 55 60 Thr Asn Val Val Thr Asp Ser Ala Cys Ala Ser Thr Ser Gly Ser Trp 65 70 75 80 Tyr Ser Thr Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val Asp 85 90 95 Ile Asp His Val Pro Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala Ala 100 105 110 Ser Trp Thr Thr Ala Arg Arg Gin Ser Phe Ala Asn Asp Leu Thr Asn H5 120 125 Pro Gin Leu Ile Ala Val Thr Asp Ser Val Asn Gin Ser Lys Gly Asp 130 135 140 Lys Gly Pro Glu Ser Trp Lys Pro Pro Leu Thr Ser Tyr His Cys Thr 145 150 155 160 Tyr Ala Lys Met Trp Val Lys Val Lys Asp Val Tyr Ser Leu Thr Val 165 170 175 Thr Ser Ala Glu Lys Ser Ala Leu Thr Thr With Leu Asn Thr Cys 180 185 190 <210> 63 <211> 192 <212> PRT <213> Phaeosphaeria sp. <4 00> 63 Leu Pro Ala Pro Ile His Leu Thr Ala Arg Ala Pro Pro Asn Ile Pro 1 5 10 15 Ser Ala Ser Glu Ala Arg Thr Gin Leu Ala Gly Leu Thr Val Ala Ala 20 25 30 Gin Gly Pro Gin Asp Gly Tyr Ser Arg Asp Leu Phe Pro His Trp Ile 35 40 45 Thr Gin Ser Gly Thr Cys Asn Thr Arg Glu Thr Val Leu Lys Arg Asp 50 55 60 Gly Thr Asn Val Val Thr Asn Ser Ala Cys Ala Ser Thr Ser Gly Ser 65 70 75 80 Trp Phe Ser Pro Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val 85 90 95 Asp Ile Asp His With Val Pro Leu Ser Asn Ala Trp List Ser Gly Ala 100 105 110 Ala Ser Trp Thr Thr Ala Arg Arg Gin Ala Phe Ala Asn Asp Leu Thr 115 120 125 Asn Pro Gin Leu Leu Ala Val Thr Asp Asn Val Asn Gin Ala Lys Gly 130 135 140 Asp Lys Gly Pro Glu Asp Trp Lys Pro Pro Leu Thr Ser Tyr Tyr Cys 145 150 155 160 Thr Tyr Ala Arg Met Trp Val Lys Val Lys Ser Val Tyr Ala Leu Thr 165 170 175 Val Thr Ser Ala Glu Lys Ser Ala Leu Thr Ser With Leu Gly Thr Cys 180 185 190 <210> 64 <211> 189 <212> PRT <213> Didymosphaeria futilis <4 0 0> 6 4 Leu Pro Thr Pro Asn Thr Leu Glu Ala Arg Ala Pro Pro Asn Ile Pro 15 10 15 Ser Thr Ser Ala Ala Gin Ser Gin Leu Ser Ala Leu Thr Val Ala Ala 20 25 30 Gin Gly Pro Gin Thr Gly Tyr Ser Arg Asp Leu Phe Pro His Trp Ile 35 40 45 Thr Gin Ser Gly Thr Cys Asn Thr Arg Glu Thr Val Leu Lys Arg Asp 50 55 60 Gly Thr Asn Val Leu Thr Asp Ser Ala Cys Ala Ser Thr Ser Gly Ser 65 70 75 80 Trp Lys Ser Pro Tyr Asp Gly Ala Thr Trp Thr Ala Ala Ser Asp Val 85 90 95 Asp Ile Asp His Val Val Pro Leu Ser Asn Ala Trp Lys Ser Gly Ala 100 105 110 Ala Ser Trp Thr Thr Ala Arg Arg Gin Ser Phe Ala Asn Asp Leu Thr 115 120 125 Asn Pro Gin Leu Ile Ala Val Thr Asp Asn Val Asn Gin Ala Lys Gly 130 135 140 Asp Lys Gly Pro Glu Asp Trp Lys Pro Pro Leu Thr Ser Tyr Tyr Cys 145 150 155 160 Thr Tyr Ala Lys With Trp Val Lys Val Lys Ser Val Tyr Ser Leu Thr 165 170 175 Ile Thr Ser Ala Glu Lys Ser Ala Leu Thr Met Leu Ala 180 185 <210> 65 <211> 109 <212> PRT <213> Bacillus licheniformis <4 00> 65 Ala Arg Tyr Asp Asp Leu Tyr Phe Pro Ala Ser Arg Tyr Pro Glu 15 10 15 Thr Gly Ala His Ile Ser Asp Ala Ile Lys Ala Gly His Ser Asp Val 20 25 30 Cys Thr Ile Glu Arg Ser Gly Ala Asp List Arg Arg Gin Glu Ser Leu 35 40 45 Lys Gly Ile Pro Thr Lys Pro Gly Phe Asp Arg Asp Glu Trp Pro Met 50 55 60 Ala Met Cys Glu Glu Gly Gly List Gly Ala Ser Val Arg Tyr Val Ser 65 70 75 80 Ser Ser Asp Asn Arg Gly Ala Gly Ser Trp Val Gly Asn Arg Leu Ser 85 90 95 Gly Phe Ala Asp Gly Thr Arg He Leu Phe Ile Val Gin 100 105 <210> 66 <211> 110 <212> PRT <213> Bacillus subtilis <4 00> 66 Ala Ser Ser Tyr Asp Lys Val Leu Tyr Phe Pro Leu Ser Arg Tyr Pro 1 5 10 15 Glu Thr Gly Ser His lie Arg Asp Ala lie Ala Glu Gly His Pro Asp 20 25 30 He Cys Thr He Asp Arg Asp Gly Ala Asp List Arg Arg Glu Glu Ser 35 40 45 Leu List Gly He Pro Thr List Pro Gly Tyr Asp Arg Asp Glu Trp Pro 50 55 60 With Ala Val Cys Glu Glu Gly Gly Ala Gly Ala Asp Val Arg Tyr Val 65 70 75 80 Thr Pro Ser Asp Asn Arg Gly Ala Gly Ser Trp Val Gly Asn Gin Met 85 90 95 Ser Ser Tyr Pro Asp Gly Thr Arg Val Leu Phe He Val Gin 100 105 no <210> 67 <211> 221 <212> PRT <213> Aspergillus oryzae <4 00> 67 Val Pro Val Asn Pro Glu Pro Asp Ala Thr Ser Val Glu Asn Val Ala 1 5 10 15 Leu Lys Thr Gly Ser Gly Asp Ser Gin Ser Asp Pro Ile Lys Ala Asp 20 25 30 Leu Glu Val Lys Gly Gin Ser Ala Leu Pro Phe Asp Val Asp Cys Trp 35 40 45 Ala Ile Leu Cys Lys Gly Ala Pro Asn Val Leu Gin Arg Val Asn Glu 50 55 60 Lys Thr Lys Asn Ser Asn Arg Asp Arg Ser Gly Ala Asn Lys Gly Pro 65 70 75 80 Phe Lys Asp Pro Gin Lys Trp Gly Ile Lys Ala Leu Pro Pro Lys Asn 85 90 95 Pro Ser Trp Ser Ala Gin Asp Phe Lys Ser Pro Glu Glu Tyr Ala Phe 100 105 110 Ala Ser Ser Leu Gin Gly Gly Thr Asn Ala Ile Leu Ala Pro Val Asn 115 120 125 Leu Ala Ser Gin Asn Ser Gin Gly Gly Val Leu Asn Gly Phe Tyr Ser 130 135 140 Ala Asn Lys Val Ala Gin Phe Asp Pro Ser Lys Pro Gin Gin Thr Lys 145 150 155 igg Gly Thr Trp Phe Gin Ile Thr Lys Phe Thr Gly Ala Ala Gly Pro Tyr 165 170 175 Cys Lys Ala Leu Gly Ser Asn Asp Lys Ser Val Cys Asp Lys Asn Lys 180 105 290 Asn Ile Ala Gly Asp Trp Gly Phe Asp Pro Ala Lys Trp Ala Tyr Gin 195 200 205 Tyr Asp Gl u List Asn Asn List Phe Asn Tyr Val Gly List 210 215 220 <210> 68 <211> 188 <212> PRT <213> Trichoderma harzianum <400> 68 Ala Pro Ala Pro With Pro Thr Pro Pro Gly Ile Pro Thr Glu Ser Ser 15 10 15 Ala Arg Thr Gin Leu Ala Gly Leu Thr Val Ala Val Ala Gly Ser Gly 20 25 30 Thr Gly Tyr Ser Arg Asp Leu Phe Pro Thr Trp Asp Ala Ile Ser Gly 35 40 45 Asn Cys Asn Ala Arg Glu Tyr Val Leu Lys Arg Asp Gly Glu Gly Val 50 55 60 Gin Val Asn Asn Ala Cys Glu Ser Gin Ser Gly Thr Trp Ile Ser Pro 65 70 75 80 Tyr Asp Asn Ala Ser Phe Thr Asn Ala Ser Ser Leu Asp Ile Asp His 85 90 95 With Val Pro Leu List Asn Ala Trp Ile Ser Gly Ala Ser Ser Trp Thr 100 105 110 Thr Ala Gin Arg Glu Ala Leu Ala Asn Asp Val Ser Arg Pro Gin Leu 115 120 125 Trp Ala Val Ser Ala Ser Ala Asn Arg Ser Lys Gly Asp Arg Ser Pro 130 135 140 Asp Gin Trp List Pro Pro Leu Thr Ser Phe Tyr Cys Thr Tyr Ala Lys 145 150 155 160 Ser Trp Ile Asp Val Lys Ser Phe Tyr Lys Leu Thr Ile Thr Ser Ala 165 170 175 Glu Lys Thr Ala Leu Ser Ser With Leu Asp Thr Cys 180 185 <210> 69 <211> 269 <212> PRT <213> bacillus lentus <4 00> 69 Ala Gin Ser Val Pro Trp Gly Ile Ser Arg Val Gin Ala Pro Ala Ala 15 10 15 His Asn Arg Gly Leu Thr Gly Ser Gly Val Lys Val Ala Val Leu Asp 20 25 30 Thr Gly Ile Ser Thr His Pro Asp Leu Asn Ile Arg Gly Gly Ala Ser 35 40 45 Phe Val Pro Gly Glu Pro Ser Thr Gin Asp Gly Asn Gly His Gly Thr 50 55 60 His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu 65 70 75 so Gly Val Ala Pro Ser Ala Glu Leu Tyr Ala Val List Val Leu Gly Ala 85 90 95 Ser Gly Ser Gly Ser Val Ser Ser Ile Ala Gin Gly Leu Glu Trp Ala 100 105 110 Gly Asn Asn Gly With His Val Ala Asn Leu Ser Leu Gly Ser Pro Ser 115 120 125 Pro Ser Ala Thr Leu Glu Gin Ala Val Asn Ser Ala Thr Ser Arg Give 130 135 140 Val Leu Val Val Ala Ala Ser Gly Asn Ser Gly Ala Gly Ser Ile Ser 145 150 155 i6o Tyr Pro Ala Arg Tyr Ala Asn Ala With Ala Val Gly Ala Thr Asp Gin 165 170 175 Asn Asn Asn Arg Ala Ser Phe Ser Gin Tyr Gly Ala Gly Leu Asp Ile 180 185 190 Val Ala Pro Gly Val Asn Val Gin Ser Thr Tyr Pro Gly Ser Thr Tyr 195 200 205 Ala Ser Leu A sn Gly Thr Ser With Ala Thr Pro His Val Ala Gly Ala 210 215 220 Ala Ala Leu Val Lys Gin Lys Asn Pro Ser Trp Ser Asn Val Gin lie 225 230 235 240 Arg Asn His Leu Lys Asn Thr Ala Thr Ser Leu Gly Ser Thr Asn Leu 245 250 255 Tyr Gly Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 260 265 <210> 70 <211> 275 <212> PRT <213> Bacillus amyloliquefaciens <4 00> 70 Ala Gin Ser Val Pro Tyr Gly Val Ser Gin He Lys Ala Pro Ala Leu 1 5 10 15 His Ser Gin Gly Tyr Thr Gly Ser Asn Val Lys Val Ala Val lie Asp 20 25 30 Ser Gly He Asp Ser Ser His Pro Asp Leu List Val Ala Gly Gly Ala 35 40 45 Ser With Val Pro Ser Glu Thr Asn Pro Phe Gin Asp Asn Asn Ser His 50 55 60 Gly Thr His Val Ala Gly Thr Val Ala Ala Leu Asn Asn Ser lie Gly 65 70 75 80 Val Leu Gly Val Ala Pro Ser Ala Ser Leu Tyr Ala Val List Val Leu 85 90 95 Gly Ala Asp Gly Ser Gly Gin Tyr Ser Trp He He Asn Gly lie Glu 100 105 110 Trp Ala lie Ala Asn Asn With Asp Val He Asn Met Ser Leu Gly Gly 115 120 125 Pro Ser Gly Ser Ala Ala Leu List Ala Ala Val Asp List Ala Val Ala 130 135 140 Ser Gly Val Val Val Ala Ala Ala Gly Asn Glu Gly Thr Ser Gly 145 150 155 160 Ser Ser Ser Thr Val Gly Tyr Pro Gly List Tyr Pro Ser Val lie Ala 165 170 175 Val Gly Ala Val Asp Ser Ser Asn Gin Arg Ala Ser Phe Ser Ser Val 180 185 190 Gly Pro Glu Leu Asp Val With Ala Pro Gly Val Ser lie Gin Ser Thr 195 200 205 Leu Pro Gly Asn L ice Tyr Gly Ala Tyr Asn Gly Thr Ser With Ala Ser 210 215 220 Pro His Val Ala Gly Ala Ala Ala Leu lie Leu Ser Lys His Pro Asn 225 230 235 240 Trp Thr Asn Thr Gin Val Arg Ser Ser Leu Glu Asn Thr Thr Thr Lys 245 250 255 Leu Gly Asp Ser Phe Tyr Tyr Gly Lys Gly Leu lie Asn Val Gin Ala 260 265 270 Ala Ala Gin 275 <210> 71 <211> 311 <212> PRT <213> Bacillus subtilis <4 00> 71 Ala Val Pro Ser Thr Gin Thr Pro Gly lie Lys Ser lie Tyr Asn 15 10 15 Asp Gin Ser lie Thr Lys Thr Thr Gly Ser Gly lie Lys Val Ala 20 25 30 Val Leu Asp Thr Gly Val Tyr Thr Ser His Leu Asp Leu Ala Gly Ser 35 40 45 Ala Glu Gin Cys Lys Asp Phe Thr Gin Ser Asn Pro Leu Val Asp Give 50 55 60 Ser Cys Thr Asp Arg Gin His Gly Thr His Val Ala Gly Thr Val 65 70 75 80 Leu Ala His Gly Gly Ser Asn Gly Gin Gly Val Tyr Gly Val Ala Pro 90 95 Gin Ala Lys Leu Trp Ala Tyr Lys Val Leu Gly Asp Asn Gly Ser Gly 100 105 110 Tyr Ser Asp Asp Ile Ala Ala Ile Arg His Val Ala Asp Glu Ala 115 120 125 Ser Arg Thr Gly Ser Lys Val Val Ile Asn Met Ser Leu Gly Ser Ser 130 135 i4o Ala Lys Asp Ser Leu Ile Ala Ser Ala Val Asp Tyr Ala Tyr Gly Lys 145 150 155 160 Gly Val Leu Ile Val Ala Ala Ala Gly Asn Ser Gly Ser Gly Ser Asn 165 170 175 Thr Ile Gly Phe Pro Gly Gly Leu Val Asn Ala Val Ala Val Ala Ala 180 185 190 Leu Glu Asn Val Gin Gin Asn Gly Thr Tyr Arg Val Ala Asp Phe Ser 195 200 205 Ser Arg Gly Asn Pro Ala Thr Ala Gly Asp Tyr Ile Gin Glu Arg 210 215 220 Asp Ile Glu Val Ser Ala Pro Gly Ala Ser Val Glu Ser Thr Trp Tyr 225 230 235 240 Thr Gly Gly Tyr Asn Thr Ile Ser Gly Thr Ser With Ala Thr Pro His 245 250 255 Val Ala Gly Leu Ala Ala Lys Ile Trp Ser Ala Asn Thr Ser Leu Ser 260 265 270 His Ser Gin Leu Arg Thr Glu Leu Gin Asn Arg Ala Lys Val Tyr Asp 275 280 285 Ile Lys Gly Gly Ile Gly Ala Gly Thr Gly Asp Asp Tyr Ala Ser Gly 290 295 300 Phe Gly Tyr Pro Arg Val List 305 310 <210> 72 <211> 298 <212> PRT <213> Bacillus bogoriensis <4 00> 72 Ala Asn Ser Gly Phe Tyr Val Ser Gly Thr Thr Leu Tyr Asp Ala Asn 15 10 15 Gly Asn Pro Phe Val With Arg Gly Ile Asn His Gly His Ala Trp Tyr 20 25 30 Lys Asp Gin Ala Thr Thr Ala Ile Glu Gly Ile Ala Asn Thr Gly Ala 35 40 45 Asn Thr Val Arg Ile Val Leu Ser Asp Gly Gly Gin Trp Thr Lys Asp 50 55 60 Asp Ile His Thr Val Arg Asn Leu Ile Ser Leu Ala Glu Asp Asn His 65 70 75 80 Leu Val Ala Val Leu Glu Val His Asp Ala Thr Gly Tyr Asp Ser Ile 85 90 95 Ala Ser Leu Asn Arg Ala Val Asp Tyr Trp Ile Glu With Arg Ser Ala 100 105 110 Leu Ile Gly List Glu Asp Thr Val Ile Asn Ile Ala Asn Glu Trp 115 120 125 Phe Gly Ser Trp Glu Gly Asp Ala Trp Ala Asp Gly Tyr Lys Gin Ala 130 135 140 lie Pro Arg Leu Arg Asn Ala Gly Leu Asn His Thr Leu With Val Asp 145 150 155 160 Ala Ala Gly Trp Gly Gin Phe Pro Gin Ser Ile His Asp Tyr Gly Arg 165 170 175 Glu Val Phe Asn Ala Asp Pro Gin Arg Asn Thr Met Phe Ser He His 180 185 190 Met Tyr Glu Tyr Ala Gly Gly Asn Ala Ser Gin Val Arg Thr Asn lie 195 200 205 Asp Arg Val Le u Asn Gin Asp Leu Ala Leu Val He Gly Glu Phe Gly 210 215 220 His Arg His Thr Asn Gly Asp Val Asp Glu Ala Thr He With Ser Tyr 225 230 235 240 Ser Glu Gin Arg Gly Val Gly Trp Leu Ala Trp Ser Trp List Gly Asn 245 250 255 Gly Pro Glu Trp Glu Tyr Leu Asp Leu Ser Asn Asp Trp Ala Gly Asn 260 265 270 Asn Leu Thr Ala Trp Gly Asn Thr He Val Asn Gly Pro Tyr Gly Leu 275 280 285 Arg Glu Thr Ser Arg Leu Ser Thr Val Phe 290 295 <210> 73 <211> 278 <212> PRT <213> thielavia terrestris <4 00> 73 Ala Ser Gly Ser Gly Gin Ser Thr Arg Tyr Trp Asp Cys Cys List Pro 1 5 10 15 Ser Cys Ala Trp Pro Gly Lys Ala Ala Val Ser Gin Pro Val Tyr Ala 20 25 30 Cys Asp Ala Asn Phe Gin Arg Leu Ser Asp Phe Asn Val Gin Ser Gly 35 40 45 Cys Asn Gly Gly Ser Ala Tyr Ser Cys Ala Asp Gin Thr Pro Trp Ala 50 55 60 Val Asn Asp Asn Leu Ala Tyr Gly Phe Ala Ala Thr Ser Ile Ala Gly 65 70 75 80 Gly Ser Glu Ser Ser Trp Cys Cys Ala Cys Tyr Ala Leu Thr Phe Thr 85 90 95 Ser Gly Pro Val Ala Gly List Thr With Val Val Gin Ser Thr Ser Thr 100 105 110 Gly Gly Asp Leu Gly Ser Asn Gin Phe Asp Ile Ala With Pro Gly Gly 120 125 Gly Val Gly Ile Phe Asn Gly Cys Ser Ser Gin Phe Gly Gly Leu Pro 130 135 140 Gly Ala Gin Tyr Gly Gly Ile Ser Ser Arg Asp Gin Cys Asp Ser Phe 145 150 155 160 Pro Ala Pro Leu Lys Pro Gly Cys Gin Trp Arg Phe Asp Trp Phe Gin 165 170 175 Asn Ala Asp Asn Pro Thr Phe Thr Phe Gin Val Gin Cys Pro Ala 180 185 190 Glu Ile Val Ala Arg Ser Gly Cys Lys Arg Asn Asp Asp Ser Ser Phe 195 200 205 Pro Val Phe Thr Pro Pro Ser Gly Gly Asn Gly Gly Thr Gly Thr Pro 21 ° 215 220 Thr Ser Thr Ala Pro Gly Ser Gly Gin Thr Ser Pro Gly Gly Gly Ser ^ 230 235 240 Gly Cys Thr Ser Gin Lys Trp Ala Gin Cys Gly Gly Ile Gly Phe Ser 245 250 255 Gyy Cys Thr Thr Cys Val Ser Gly Thr Thr Cys Gin Lys Leu Asn Asp 260 265 270 Tyr Phe Ser Gin Cys Leu 275 <210> 74 <211> 470 <212> PRT <213> humicola insolence <4 00> 74 With Arg Ser Ser Pro Leu Arg Ser Ala Val Val Ala Ala Leu Pro 1 5 10 15 Val Leu Ala Leu Ala Ala Asp Gly Arg Ser Thr Arg Tyr Trp Asp Cys 20 25 30 Cys List Pro Ser Cys Gly Trp Ala Lys List Ala Pro Val Asn Gin Pro 35 40 45 Val Phe Ser Cys Asn Ala Asn Phe Gin Arg Ile Thr Asp Phe Asp Ala 50 55 60 Lys Ser Gly Cys Glu Pro Gly Gly Val Ala Tyr Ser Cys Ala Asp Gin 65 70 75 g0 Thr Pro Trp Ala Val Asn Asp Asp Phe Ala Leu Gly Phe Ala Ala Thr 85 90 95 Ser Ile Ala Gly Ser Asn Glu Ala Gly Trp Cys Cys Ala Cys Tyr Glu 100 105 110 Leu Thr Phe Thr Ser Gly Pro Val Ala Gly List List With Val Val Gin 115 120 125 Ser Thr Ser Thr Gly Gly Asp Leu Gly Ser Asn His Phe Asp Leu Asn 150 135 240 Ile Pro Gly Gly Gly Val Gly Ile Phe Asp Gly Cys Thr Pro Gin Phe 145 150 155 160 Gly Gly Leu Pro Gly Gin Arg Tyr Gly Gly Ile Ser Ser Arg Asn Glu! 65 170 175 Cys Asp Arg Phe Pro Asp Ala Leu List Pro Gly Cys Tyr Trp Arg Phe 180 185 190 Asp Trp Phe List Asn Ala Asp Asn Pro Ser Phe Ser Phe Arg Gin Val 195 200 205 Gin Cys Pro Ala Glu Leu Val Ala Arg Thr Gly Cys Arg Arg Asn Asp 210 215 220 Asp Gly Asn Phe Pro Ala Val Gin Ile Pro Ser Ser Ser Thr Ser Ser 225 230 235 240 Pro Val Asn Gin Pro Thr Ser Thr Thr Thr Ser Thr Ser Thr Thr 245 250 255 Ser Ser Pro Pro Val Gin Pro Thr Thr Pro Ser Gly Cys Ala Asp Gly 260 265 270 Arg Ser Thr Arg Tyr Trp Asp Cys Cys List Pro Ser Cys Gly Trp Ala 275 280 285 Lys List Ala Pro Val Asn Gin Pro Val Phe Ser Cys Asn Ala Asn Phe 290 295 300 Gin Arg Ile Thr Asp Phe Asp Ala Lys Ser Gly Cys Glu Pro Gly Gly 305 310 315 320 Val Ala Tyr Ser Cys Ala Asp Gin Thr Pro Trp Ala Val Asn Asp Asp 325 330 335 Phe Ala Leu Gly Phe Ala Ala Thr Ser Ile Ala Gly Ser Asn Glu Ala 340 345 350 Gly Trp Cys Cys Ala Cys Tyr Glu Leu Thr Phe Thr Ser Gly Pro Val 355 360 365 Ala Gly List List With Val Val Gin Ser Thr Ser Thr Gly Gly Asp Leu 37 ° 375 380 Gly Ser Asn His Phe Asp Leu Asn Ile Pro Gly Gly Gly Val Gly Ile 385 390 395 400 Phe Asp Gly Cys Thr Pro Gin Phe Gly Gly Leu Pro Gly Gin Arg Tyr 405 410 415 Gly Gly Ile Ser Ser Arg Asn Glu Cys Asp Arg Phe Pro Asp Ala Leu 420 425 430 Lys Pro Gly Cys Tyr Trp Arg Phe Asp Trp Phe Lys Asn Ala Asp Asn 435 440 445 Pro Ser Phe Ser Phe Arg Gin Val Gin Cys Pro Ala Glu Leu Val Ala 450 455 460 Arg Thr Gly Cys Arg Arg 465 470 <210> 75 <211> 425 <212> PRT <213> Escherichia coli <4 00> 75 Thr Ala Leu Leu Gly Leu Val Asn Gly Gin Lys Pro Gly Glu Thr 15 10 15 Lys Glu Val His Pro Gin Leu Thr Thr Phe Arg Cys Thr Lys Arg Gly 20 25 30 Gly Cys Lys Pro Ala Thr Asn Phe Ile Val Leu Asp Ser Leu Ser His 35 40 45 Pro Ile His Arg Ala Glu Gly Leu Gly Pro Gly Gly Cys Gly Asp Trp 50 55 60 Gly Asn Pro Pro List Asp Val Cys Pro Asp Val Glu Ser Cys Ala 65 70 75 80 List Asn Cys Ile With Glu Gly Ile Pro Asp Tyr Ser Gin Tyr Gly Val 85 90 95 Thr Thr Asn Gly Thr Ser Leu Arg Leu Gin His Ile Leu Pro Asp Gly 100 105 110 Arg Val Pro Ser Pro Arg Val Tyr Leu Asp Lys Thr Lys Arg Arg 115 120 125 Tyr Glu With Leu His Leu Thr Gly Phe Glu Phe Thr Phe Asp Val Asp 130 135 140 Ala Thr Lys Leu Pro Cys Gly With Asn Ser Ala Leu Tyr Leu Ser Glu 145 150 155 160 With His Pro Thr Gly Ala Lys Ser Lys Tyr Asn Pro Gly Gly Ala Tyr 165 170 175 Tyr Gly Thr Gly Tyr Cys Asp Ala Gin Cys Phe Val Thr Pro Phe Ile 180 185 190 Asn Gly Leu Gly Asn Ile Glu Gly Lys Gly Ser Cys Cys Asn Glu Met! 95 200 205 Asp Ile Trp G lu Ala Asn Ser Arg Ala Ser His Val Ala Pro His Thr 210 215 220 Cys Asn Light List Gly Leu Tyr Leu Cys Glu Gly Glu Glu Cys Ala Phe 225 230 235 240 Glu Gly Val Cys Asp Lys Asn Gly Cys Gly Trp Asn Asn Tyr Arg Val 245 250 255 Asn Val Thr Asp Tyr Tyr Gly Arg Gly Glu Glu Phe Lys Val Asn Thr 2 ^ 0 265 270 Leu Lys Pro Phe Thr Val Val Thr Thr Gin Phe Leu Ala Asn Arg Arg Gly 275 280 285 Lys Leu Glu Lys Ile His Arg Phe Tyr Val Gin Asp Gly List Val Ile 290 295 300 Glu Ser Phe Tyr Thr Asn List Glu Gly Val Pro Tyr Thr Asn Met Ile 305 Ήη 315 320 Asp Asp Glu Phe Cys Glu Ala Thr Gly Ser Arg List Tyr With Glu Leu 325 330 335 Gly Ala Thr Gin Gly With Gly Glu Ala Leu Thr Arg Gly With Val Leu 340 345 350 Ala With Ser He Trp Trp Asp Gin Gly Gly Asn With Glu Trp Leu Asp 355 360 365 His Gly Glu Ala Gly Pro Cys Ala List Gly Glu Gly Ala Pro Ser Asn 370 375 380 lie Val Gin Val Glu Pro Phe Pro Glu Val Thr Tyr Thr Asn Leu Arg 385 390 395 400 Trp Gly Glu He Gly Ser Thr Tyr Gin Glu Val Gin Lys Pro Lys Pro 405 410 415 Lys Pro Gly Hi s Gly Pro Arg Ser Asp 420 425 <210> 76 <211> 773 <212> PRT <213> bacillus subtilis <4 00> 76 Ala Glu Gly Asn Thr Arg Glu Asp Asn Phe List His Leu Leu Gly Asn 1 5 10 15 Asp Asn Val List Arg Pro Ser Glu Ala Gly Ala Leu Gin Leu Gin Glu 20 25 30 Val Asp Gly Gin With Thr Leu Val Asp Gin His Gly Glu List He Gin 35 40 45 Leu Arg Gly With Ser Thr His Gly Leu Gin Trp Phe Pro Glu He Leu 50 55 60 Asn Asp Asn Ala Tyr List Ala Leu Ala Asn Asp Trp Glu Ser Asn Met 65 70 75 80 Ile Arg Leu Ala With Tyr Val Gly Glu Asn Gly Tyr Ala Ser Asn Pro 85 90 95 Glu Leu Ile List Ser Arg Val Ile List Gly Ile Asp Leu Ala Ile Glu 100 105 110 Asn Asp With Tyr Val Ile Val Asp Trp His Val His Ala Pro Gly Asp 115 120 125 Pro Arg Asp Pro Val Tyr Ala Gly Ala Glu Asp Phe Arg Asp Ile 130 135 140 Ala Ala Leu Tyr Pro Asn Asn Pro His Ile Ile Tyr Glu Leu Ala Asn 145 150 155 160 Glu Pro Ser Ser Asn Asn Gly Gly Ally Gly Ile Pro Asn Asn Glu 165 170 175 Glu Gly Trp Asn Ala Val List Glu Tyr Ala Asp Pro Ile Val Glu Met 180 185 190 Leu Arg Asp Ser Gly Asn Ala Asp Asp Asn Ile Ile Val Gly Ser 195 200 205 Pro Asn Trp Se r Gin Arg Pro Asp Leu Ala Ala Asp Asn Pro Ile Asn 210 215 220 Asp His His Thr With Tyr Thr Val His Phe Tyr Thr Gly Ser His Ala 225 230 235 240 Ala Ser Thr Glu Ser Tyr Pro Pro Glu Thr Pro Asn Ser Glu Arg Gly 245 250 255 Asn Val With Ser Asn Thr Arg Tyr Ala Leu Glu Asn Gly Val Ala Val 260 265 270 Phe Ala Thr Glu Trp Gly Thr Ser Gin Ala Asn Gly Asp Gly Gly Pro 275 280 285 Tyr Phe Asp Glu Ala Asp Val Trp Ile Glu Phe Leu Asn Glu Asn Asn 290 295 300 Ile Ser Trp Ala Asn Trp Ser Leu Thr Asn List Asn Glu Val Ser Gly 305 310 315 320 Ala Phe Thr Pro Phe Glu Leu Gly List Ser Asn Ala Thr Asn Leu Asp 325 330 335 Pro Gly Pro Asp His Val Trp Ala Pro Glu Glu Leu Ser Leu Ser Gly 340 345 350 Glu Tyr Val Arg Ala Arg Ile Lys Gly Val Asn Tyr Glu Pro Ile Asp 355 360 365 Arg Thr Lys Tyr Thr Lys Val Leu Trp Asp Phe Asn Asp Gly Thr List 37 ° 375 380 Gin Gly Phe Gly Val Asn Ser Asp Ser Pro Asn List Glu Leu Ile Ala 385 390 395 400 Val Asp Asn Glu Asn Asn Thr Leu Lys Val Ser Gly Leu Asp Val Ser 405 410 415 Asn Asp Val S is Asp Gly Asn Phe Trp Ala Asn Ala Arg Leu Ser Ala 420 425 430 Asp Gly Trp Gly List Ser Val Ile Leu Gly Ala Glu List Leu Thr 435 440 445 With Asp Val Ile Val Asp Glu Pro Thr Thr Val Ala Ile Ala Ala Ile 450 455 460 Pro Gin Ser Ser Lys Ser Gly Trp Ala Asn Pro Glu Arg Ala Val Arg 465 470 475 480 Val Asn Ala Glu Asp Phe Val Gin Gin Thr Asp Gly List Tyr Lys Ala 485 490 495 Gly Leu Thr Ile Thr Gly Glu Asp Ala Pro Asn Leu List Asn Ile Ala 500 505 510 Phe His Glu Glu Asp Asn Asn With Asn Asn Ile Leu Phe Val Gly 515 520 525 Thr Asp Ala Asp Asp Val Ile Tyr Leu Asp Asn Ile Lys Val Ile Gly 530 535 540 Thr Glu Val Glu Ile Pro Val Val His Asp Pro List Gly Glu Ala Val 545 550 555 560 Leu Pro Ser Val Phe Glu Asp Gly Thr Arg Gin Gly Trp Asp Trp Ala 565 570 575 Gly Glu Ser Gly Val Lys Thr Ala Leu Thr Ile Glu Glu Ala Asn Gly 580 585 590 Ser Asn Ala Leu Ser Trp Glu Phe Gly Tyr Pro Glu Val Light Pro Ser 595 600 605 Asp Asn Trp Ala Thr Ala Pro Arg Leu Asp Phe Trp Light Ser Asp Leu 610 615 620 Soil Arg Gly GluAsn Asp Tyr Val Ala Phe Asp Phe Tyr Leu Asp Pro 625 630 635 640 Val Arg Ala Thr Glu Gly Ala With Asn Ile Asn Leu Val Phe Gin Pro 645 650 655 Pro Thr Asn Gly Tyr Trp Val Gin Ala Pro List Thr Tyr Thr Ile Asn 660 665 670 Phe Asp Glu Leu Glu Glu Ala Asn Gin Val Asn Gly Leu Tyr His Tyr 675 680 685 Glu Val Lys Ile Asn Val Arg Asp Ile Thr Asn Ile Gin Asp Asp Thr 690 695 700 Leu Leu Arg Asn With Ile Ile Phe Ala Asp Val Glu Ser Asp Phe 705 710 715 720 Ala Gly Arg Val Phe Val Asp Asn Val Arg Phe Glu Gly Ala Thr 725 730 735 Thr Glu Pro Val Glu Pro Glu Pro Val Asp Pro Gly Glu Glu Thr Pro 740 745 750 Pro Val Asp Glu List Glu Ala List List Glu Gin List Glu Ala Glu List 755 760 765 Glu Glu List Glu Glu 770 <210> 77 <211> 524 <212> PRT <213> Paenibacillus polymyxa <400> 77 Val Val His Gly Gin Thr Ala Lys Thr Ile Thr Ile Lys Val Asp Thr 1 5 10 15 Phe Lys Asp Arg Arg Pro Ile Ser Pro Tyr Ile Tyr Gly Thr Asn Gin 20 25 30 Asp Leu Ala Gly Asp Glu Asn With Ala Ala Arg Arg Leu Gly Gly Asn 35 40 45 Arg With Thr Gly Tyr Asn Trp Glu Asn Asn With Ser Asn Ala Gly Ser 50 55 60 Asp Trp Gin His Ser Ser Asp Asn Tyr Leu Cys Ser Asn Gly Leu 65 70 75 80 Thr Gin Ala Glu Cys Glu Lys Pro Gly Ala Val Val Thr Ser Phe His 85 90 95 Asp Gin Ser Leu Lys Leu Gly Thr Tyr Ser Leu Val Thr Leu Pro Met 100 105 110 Ala Gly Tyr Val Ala Ala Asp Gly Asn Gly Ser Val Gin Glu Ser Glu 115 120 125 Ala Ala Pro Ser Ala Arg Trp Asn Gin Val Val Asn Ala Lys Asn Ala 130 135 140 Pro Phe Gin Leu Gin Pro Asp Leu Asn Asp Asn Tyr Val Tyr Val Asp I45 150 155 160 Glu Phe Val His Phe Leu Val Asn Lys Tyr Gly Thr Ala Ser Thr Lys 165 170 175 Ala Gly Val Lys Gly Tyr Ala Leu Asp Asn Glu Pro Ala Leu Trp Ser 180 185 190 His Thr His Pro Arg He His Pro Glu Lys Val Gly Ala Lys Glu Leu 195 200 205 Val Asp Arg Se r Val Ser Leu Ser Lys Ala Val Lys Ala Asp Asp Ala 210 215 220 Gly Ala Glu Val Phe Gly Pro Val Leu Tyr Gly Phe Gly Ala Tyr Lys 225 230 235 240 Asp Leu Gin Thr Ala Pro Asp Trp Asp Ser Val Lys Gly Asn Tyr Ser 245 250 255 Trp Phe Val Asp Tyr Tyr Leu Asp Gin With Arg Leu Ser Ser Gin Val 260 265 270 Glu Gly List Arg Leu Leu Asp Val Phe Asp Val His Trp Tyr Pro Glu 275 280 285 Ala With Gly Gly Gly He Arg He Thr Asn Glu Val Gly Asn Asp Glu 290 295 300 Thr List List Ala Arg With Gin Ala Pro Arg Thr Leu Trp Asp Pro Thr 305 310 315 320 Tyr List Glu Asp Ser Trp lie Ala Gin Trp Phe Ser Glu Phe Leu Pro 325 330 335 He Leu Pro Arg Leu Lys Gin Ser Val Asp Tyr Tyr Pro Gly Thr 340 345 350 Lys Leu Ala Met Thr Glu Tyr Ser Tyr Gly Gly Glu Asn Asp He Ser 355 360 365 Gly Gly He Ala Met Thr Asp Val Leu Gly lie Leu Gly List Asn Asp 370 375 380 Val Tyr With Ala Asn Tyr Trp List Leu List Asp Gly Val Asn Asn Tyr 385 390 395 400 Val Ser Ala Ala Tyr List Leu Tyr Arg Asn Tyr Asp Gly List Asn Ser 405 410 415 Thr Phe Gly Asp Thr Ser Val Ser Ala Gin Thr Ser Asp Ile Val Asn 420 425 430 Ser Ser Val His Ala Ser Val Thr Asn Ala Ser Asp List Glu Leu His 435 440 445 Leu Val Val With Asn List Ser With Asp Ser Ala Phe Asp Ala Gin Phe 450 455 460 Asp Leu Ser Gly Ala Lys Thr Tyr Ile Ser Gly Lys Val Trp Gly Phe 465 470 475 480 Asp Lys Asn Ser Ser Gin Ile Lys Glu Ala Ala Pro Ile Thr Gin Ile 485 490 495 Ser Gly Asn Arg Phe Thr Tyr Thr Val Pro Pro Leu Thr Ala Tyr His 500 505 510 Ile Val Leu Thr Thr Gly Asn Asp Thr Ser Pro Val 515 520 <210> 78 <211> 485 <212> PRT <213> Bacillus sp. <4 00> 78 His Asn Gly Thr Asn Gly Thr Met Gin Tyr Phe Glu Trp Tyr 1 5 10 15 Leu Pro Asn Asp Gly Asn His Trp Asn Arg Leu Arg Ser Asp Ala Ser 20 25 30 Asn Leu Lys Asp Lys Gly Ile Ser Ala Val Trp Ile Pro Pro Ala Trp 35 40 45 Lys Gly Ala Ser Gin Asn Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr 50 55 60 Asp Leu Gly Glu Phe Asn Gin Lys Gly Thr Ile Arg Thr Lys Tyr Gly 65 70 75 80 Thr Arg Asn Gin Leu Gin Ala Ala Val Asn Ala Leu Ser Ser Asn Gly 85 90 95 lie Gin Val Tyr Gly Asp Val Val With Asn His List Gly Gly Ala Asp 100 105 HO Ala Thr Glu With Val Arg Ala Val Glu Val Asn Pro Asn Asn Arg Asn 115 120 125 Gin Glu Val Ser Gly Glu Tyr Thr He Glu Ala Trp Thr Lys Phe Asp 130 135 140 Phe Pro Gly Arg Gly Asn Thr His Ser Asn Phe Lys Trp Arg Trp Tyr 145 150 155 160 His Phe Asp Gly Val Asp Trp Asp Gin Ser Arg List Leu Asn Asn Arg 105 170 175 He Tyr List Phe Arg Gly Asp Gly List Gly Trp Asp Trp Glu Val Asp 180 185 190 Thr Glu Asn Gly Asn Tyr Asp Tyr Leu With Tyr Ala Asp He Asp Met 195 200 205 Asp His Pro Glu Val Val Asn Glu Leu Arg Asn Trp Gly Val Trp Tyr 210 215 220 Thr Asn Thr Leu Gly Leu Asp Gly Phe Arg He Asp Ala Val Lys His 225 230 235 240 He Lys Tyr Ser Phe Thr Arg Asp Trp He Asn His Val Arg Ser Ala 245 250 255 Thr Gly List Asn With Phe Ala Val Ala Glu Phe Trp List Asn Asp Leu 260 265 270 Gly Ala He Glu Asn Tyr Leu Asn List Thr Asn Trp Asn His Ser Val 275 280 285 Phe Asp Val Pro Leu His Tyr Asn Leu Tyr Asn Ala Ser List Ser Gly 290 295 300 Gly Asn Tyr Asp With Arg Gin Ile Phe Asn Gly Thr Val Val Gin Arg 305 310 315 320 His Pro With His Ala Val Thr Phe Val Asp Asn His Asp Ser Gin Pro 325 330 335 Glu Glu Ala Leu Glu Ser Phe Val Glu Glu Trp Phe List Pro Leu Ala 340 345 350 Tyr Ala Leu Thr Leu Thr Arg Glu Gin Gly Tyr Pro Ser Val Phe Tyr 355 360 365 Gly Asp Tyr Tyr Gly He Pro Thr His Gly Val Pro Ala Met Lys Ser 370 375 380 Lys lie Asp Pro lie Leu Glu Ala Arg Gin Lys Tyr Ala Tyr Gly Arg 335 390 395 400 Gin Asn Asp Tyr Leu Asp His His Asn He He Gly Trp Thr Arg Glu 405 410 415 Gly Asn Thr Ala His Pro Asn Ser Gly Leu Ala Thr He With Ser Asp 420 425 430 Gly Ala Gly Gly Asn Lys Trp With Phe Val Gly Arg Asn Lys Ala Gly 435 440 445 Gin Val Trp Thr Asp He Thr Gly Asn Arg Ala Gly Thr Val Thr He 450 455 460 Asn Ala Asp Gly Trp Gly Asn Phe Ser Val Asn Gly Gly Ser Val Ser 465 47 ° 475 480 He Trp Val Asn List 485 <210> 79 <211> 485 <212> PRT <213> Bacillus sp. NCIB 12513 <400> 79 His His Asn Gly Thr Asn Gly Thr Met Gin Tyr Phe Glu Trp His 1 5 10 15 Leu Pro Asn Asp Gly Asn His Trp Asn Arg Leu Arg Asp Asp Ala Ser 20 25 30 Asn Leu Arg Asn Arg Gly Ile Thr Ala Ile Trp Ile Pro Pro Ala Trp 35 40 45 Lys Gly Thr Ser Gin Asn Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr 50 55 60 Asp Leu Gly Glu Phe Asn Gin Lys Gly Thr Val Arg Thr Lys Tyr Gly 65 70 75 80 Thr Arg Ser Gin Leu Glu Ser Ala Ile His Ala Leu Lys Asn Asn Gly 85 90 95 Val Gin Val Tyr Gly Asp Val Val With Asn His List Gly Gly Ala Asp 100 105 110 Ala Thr Glu Asn Val Leu Ala Val Glu Val Asn Pro Asn Asn Arg Asn H5 120 125 Gin Glu Ile Ser Gly Asp Tyr Thr Ile Glu Ala Trp Thr Lys Phe Asp 130 135 140 Phe Pro Gly Arg Gly Asn Thr Tyr Ser Asp Phe Lys Trp Arg Trp Tyr I45 150 155 160 His Phe Asp Gly Val Asp Trp Asp Gin Ser Arg Gin Phe Gin Asn Arg 165 170 175 Ile Tyr List Phe Arg Gly Asp Gly List Ala Trp Asp Trp Glu Val Asp 180 185 190 Ser Glu Asn Gly Asn Tyr Asp Tyr Leu With Tyr Ala Asp Val Asp With 195 200 205 Asp His Pro Gl u Val Val Asn Glu Leu Arg Arg Trp Gly Glu Trp Tyr 210 215 220 Thr Asn Thr Leu Asn Leu Asp Gly Phe Arg Ile Asp Ala Val Lys His 225 230 235 240 Ile Lys Tyr Ser Phe Thr Arg Asp Trp Leu Thr His Val Arg Asn Ala 245 250 255 Thr Gly List Glu With Phe Ala Val Ala Glu Phe Trp List Asn Asp Leu 260 265 270 Gly Ala Leu Glu Asn Tyr Leu Asn List Thr Asn Trp Asn His Ser Val 275 280 285 Phe Asp Val Pro Leu His Tyr Asn Leu Tyr Asn Ala Ser Asn Ser Gly 290 295 300 Gly Asn Tyr Asp With Ala Lys Leu Leu Asn Gly Thr Val Val Gin Lys 305 310 315 320 His Pro With His Ala Val Thr Phe Val Asp Asn His Asp Ser Gin Pro 325 330 335 Gly Glu Ser Leu Glu Ser Phe Val Gin Glu Trp Phe Lys Pro Leu Ala 340 345 350 Tyr Ala Leu Ile Leu Thr Arg Glu Gin Gly Tyr Pro Ser Val Phe Tyr 355 360 365 Gly Asp Tyr Tyr Gly Ile Pro Thr His Ser Val Pro Ala Met Lys Ala 370 375 380 Lys Ile Asp Pro Ile Leu Glu Ala Arg Gin Asn Phe Ala Tyr Gly Thr 385 390 395 400 Gin His Asp Tyr Phe Asp His His Asn Ile Ile Gly Trp Thr Arg Glu 405 410 415 Gly Asn Thr th r His Pro Asn Ser Gly Leu Ala Thr Ile With Ser Asp 420 425 430 Gly Pro Gly Gly Glu Lys Trp With Tyr Val Gly Gin Asn Lys Ala Gly 435 440 445 Gin Val Trp His Asp Ile Thr Gly Asn Lys Pro Gly Thr Val Thr Ile 450 455 460 Asn Ala Asp Gly Trp Ala Asn Phe Ser Val Asn Gly Gly Ser Val Ser 465 470 475 480 Ile Trp Val Lys Arg 485 <210> 80 <211> 485 <212> PRT <213> Bacillus sp. no. 707 <4 00> 80 His His Asn Gly Thr Asn Gly Thr Met Gin Tyr Phe Glu Trp Tyr 1 5 10 15 Leu Pro Asn Asp Gly Asn His Trp Asn Arg Leu Asn Ser Asp Ala Ser 20 25 30 Asn Leu Lys Ser Lys Gly Ile Thr Ala Val Trp Ile Pro Pro Ala Trp 35 40 45 Lys Gly Ala Ser Gin Asn Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr 50 55 60 Asp Leu Gly Glu Phe Asn Gin Lys Gly Thr Val Arg Thr Lys Tyr Gly 65 70 75 80 Thr Arg Ser Gin Le Gin Ala Ala Val Thr Ser Leu Lys Asn Asn Gly 85 90 95 Ile Gin Val Tyr Gly Asp Val Val With Asn His List Gly Gly Ala Asp! 00 105 110 Ala Thr Glu With Val Arg Ala Val Glu Val Asn Pro Asn Asn Arg Asn H5 120 125 Gin Glu Val Thr Gly Glu Tyr Thr Ile Glu Ala Trp Thr Arg Phe Asp 130 135 140 Phe Pro Gly Arg Gly Asn Thr His Ser Ser Phe Lys Trp Arg Trp Tyr 145 150 155 160 His Phe Asp Gly Val Asp Trp Asp Gin Ser Arg Arg Leu Asn Asn Arg 165 170 175 Ile Tyr List Phe Arg Gly His Gly List Ala Trp Asp Trp Glu Val Asp 180 185 190 Thr Glu Asn Gly Asn Tyr Asp Tyr Leu With Tyr Ala Asp Ile Asp Met 195 200 205 Asp His Pro G lu Val Val Asn Glu Leu Arg Asn Trp Gly Val Trp Tyr 210 215 220 Thr Asn Thr Leu Gly Leu Asp Gly Phe Arg Ile Asp Ala Val Lys His 225 230 235 240 Ile Lys Tyr Ser Phe Thr Arg Asp Trp Ile Asn His Val Arg Ser Ala 245 250 255 Thr Gly Lys Asn With Phe Ala Val Ala Glu Phe Trp Lys Asn Asp Leu 260 265 270 Gly Ala Ile Glu Asn Tyr Leu Gin Lys Thr Asn Trp Asn His Ser Val 275 280 285 Phe Asp Val Pro Leu His Tyr Asn Leu Tyr Asn Ala Ser List Ser Gly 290 295 300 Gly Asn Tyr Asp With Arg Asn Ile Phe Asn Gly Thr Val Val Gin Arg 305 310 315 320 His Pro Ser His Ala Val Thr Phe Val Asp Asn His Asp Ser Gin Pro 325 330 335 Glu Glu Ala Leu Glu Ser Phe Val Glu Glu Trp Phe List Pro Leu Ala 340 345 350 Tyr Ala Leu Thr LeG Thr Arg Glu Gin Gly Tyr Pro Ser Val Phe Tyr 355 360 365 Gly Asp Tyr Tyr Gly He Pro Thr His Gly Val Pro Ala With Arg Ser 370 375 3go Lys lie Asp Pro He Leu Glu Ala Arg Gin List Tyr Ala Tyr Gly List 385 390 395 400 Gin Asn Asp Tyr Leu Asp His His Asn He Leu Gly Trp Thr Arg Glu 405 410 415 Gly Asn Thr Ala His Pro Asn Ser Gly Leu Ala Thr He With Ser Asp 420 425 430 Gly Ala Gly Gly Ser Lys Trp With Phe Val Gly Arg Asn Lys Ala Gly 435 440 445 Gin Val Trp Ser Asp He Thr Gly Asn Arg Thr Gly Thr Val Thr He 450 455 460 Asn Ala Asp Gly Trp Gly Asn Phe Ser Val Asn Gly Gly Ser Val Ser 465 470 475 480 He Trp Val Asn List 485 <210> 81 <211> 485 <212> PRT <213> artificial <220> <223> Protein <400> 81 His His Asp Gly Thr Asn Gly Thr He Met Gin Tyr Phe Glu Trp Asn 1 5 io 15 Val Pro Asn Asp Gly Gin His Trp Asn Arg Leu His Asn Asn Ala Gin 20 25 30 Asn Leu Lys Asn Ala Gly He Thr Ala He Trp He Pro Pro Ala Trp 35 40 45 Lys Gly Thr Ser Gin Asn Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr 50 55 bark Asp Leu Gly Glu Phe Asn Gin Lys Gly Thr Val Arg Thr Lys Tyr Gly 65 70 75 80 Thr Lys Ala Glu Leu Glu Arg Ala Ile Arg Ser Leu Lys Ala Asn Gly 85 90 95 Ile Gin Val Tyr Gly Asp Val Val With Asn His List Gly Gly Ala Asp 100 105 110 Phe Thr Glu Arg Val Gin Ala Val Glu Val Asn Pro Gin Asn Arg Asn H5 120 125 Gin Glu Val Ser Gly Thr Tyr Gin Ile Glu Ala Trp Thr Gly Phe Asn 130 135 140 Phe Pro Gly Arg Gly Asn Gin His Ser Ser Phe Lys Trp Arg Trp Tyr 145 150 155 160 His Phe Asp Gly Thr Asp Trp Asp Gin Ser Arg Gin Leu Asa Arg 165 170 175 Ile Tyr List Phe Arg Gly Asp Gly List Ala Trp Asp Trp Glu Val Asp 180 185 190 Thr Glu Asn Gly Asn Tyr Asp Tyr Leu With Tyr Ala Asp Val Asp With 195 200 205 Asp His Pro Glu V al Ile Asn Glu Leu Asn Arg Trp Gly Val Trp Tyr 210 215 220 Ala Asn Thr Leu Asn Leu Asp Gly Phe Arg Leu Asp Ala Val Lys His 225 230 235 240 Ile Lys Phe Ser Phe With Arg Asp Trp Leu Gly His Val Arg Gly Gin 245 250 255 Thr Gly Lys Asn Leu Phe Ala Val Ala Glu Tyr Trp Lys Asn Asp Leu 260 265 270 Gly Ala Leu Glu Asn Tyr Leu Ser Lys Thr Asn Trp Thr With Ser Ala 275 280 285 Phe Asp Val Pro Leu His Tyr Asn Leu Tyr Gin Ala Ser Asn Ser Ser 290 295 300 Gly Asn Tyr Asp With Arg Asn Leu Leu Asn Gly Thr Leu Val Gin Arg 305 310 315 320 His Pro Ser His Ala Val Thr Phe Val Asp Asn His Asp Thr Gin Pro 325 330 335 Gly Glu Ala Leu Glu Ser Phe Val Gin Gly Trp Phe List Pro Leu Ala 340 345 350 Tyr Ala Thr Ile Leu Thr Arg Glu Gin Gly Tyr Pro Gin Val Phe Tyr 355 360 365 Gly Asp Tyr Tyr Gly Ile Pro Ser Asp Gly Val Pro Ser Tyr Arg Gin 370 375 380 Gin Ile Asp Pro Leu Leu Ala Arg Gin Gin Tyr Ala Tyr Gly Thr 385 390 395 400 Gin His Asp Tyr Leu Asp Asn Gin Asp Val Ile Gly Trp Thr Arg Glu 405 410 415 Gly Asp Ser Ala H is Ala Gly Ser Gly Leu Ala Thr Val With Ser Asp 420 425 430 Gly Pro Gly Gly Ser Lys Thr With Tyr Val Gly Thr Ala His Ala Gly 435 440 445 Gin Val Phe Lys Asp Ile Thr Gly Asn Arg Thr Asp Thr Val Thr Ile 450 455 460 Asn Ser Ala Gly Asn Gly Thr Phe Pro Cys Asn Gly Gly Ser Val Ser 465 470 475 430 Ile Trp Val Lys Gin 485 <210> 82 <211> 600 <212> PRT <213> Curvularia verruculosa <4 00> 82 With Gly Ser Val Thr Pro Ile Pro Leu Pro Thr Ile Asp Glu Pro Glu 15 10 15 Glu Tyr Asn Asn Asn Tyr Ile Leu Phe Trp Asn Asn Val Gly Leu Glu 20 25 30 Leu Asn Arg Leu Thr His Thr Val Gly Gly Pro Leu Thr Gly Pro Pro 35 40 45 Leu Ser Ala Arg Ala Leu Gly With Leu His Leu Ala Ile His Asp Ala 50 55 60 Tyr Phe Ser Ile Cys Pro Pro Thr Glu Phe Thr Thr Phe Leu Ser Pro 65 70 75 80 Asp Ala Glu Asn Pro Ala Tyr Arg Leu Pro Ser Pro Asn Gly Ala Asp 85 90 95 Asp Ala Arg Gin Ala Val Ala Gly Ala Ala Leu List With Leu Ser Ser 100 105 110 Leu Tyr With List Pro Ala Asp Pro Asn Thr Gly Thr Asn Ile Ser Asp 115 120 125 Asn Ala Tyr Ala Gin Leu Ala Leu Val Leu Glu Arg Ala Val Val List 130 135 140 Val Pro Gly Gly Val Asp Arg Arg Glu Ser Val Ser Phe With Phe Gly Glu 145 150 155 160 Ala Val Ala Asp Val Phe Phe Ala Leu Leu Asn Asp Pro Arg Gly Ala 165 170 175 Ser Gin Glu Gly Tyr Gin Pro Thr Pro Gly Arg Tyr Lys Phe Asp Asp 180 185 190 Glu Pro Thr His Pro Val Val Leu Val Pro Val Asp Pro Asn Asn Pro 1.95 200 205 Asn Gly Pro List With Pro Phe Arg Gin Tyr His Ala Pro Phe Tyr Gly 210 215 220 With Thr Thr List Arg Phe Ala Thr Gin Ser Glu His Ile Leu Ala Asp 225 230 235 240 Pro Pro Gly Leu Arg Ser Asn Ala Asp Glu Thr Ala Glu Tyr Asp Asp 245 250 255 Ser Ile Arg Val Ala Ile Ala With Gly Gly Ala Gin Asp Leu Asn Ser 260 265 270 Thr List Arg Ser Pro Trp Gin Thr Ala Gin Gly Leu Tyr Trp Ala Tyr 275 280 285 Asp Gly Ser Asn Leu Val Gly Thr Pro Pro Arg Phe Tyr Asn Gin Ile 290 295 300 Val Arg Arg Ile Ala Val Thr Tyr List List Glu Asp Asp Leu Ala Asn 305 310 315 320 Ser Glu Val Asn Asn Ala Asp Phe Ala Arg Leu Phe Ala Leu Val Asn 325 330 335 Val Ala Cys Thr Asp Ala Gly Ile Phe Ser Trp Lys Glu Lys Trp Glu 340 345 350 Phe Glu Phe Trp Arg Pro Leu Ser Gly Val Arg Asp Asp Gly Arg Pro 355 360 365 Asp His Gly Asp Pro Phe Trp Leu Thr Leu Gly Ala Pro Ala Thr Asn 370 375 380 Thr Asn Asp Ile Pro Phe List Pro Pro Phe Pro Ala Tyr Pro Ser Gly 385 390 395 400 His Ala Thr Phe Gly Gly Ala Val Phe Gin With Val Arg Arg Tyr Tyr 405 410 415 Asn Gly ArgAsp Gly Thr Trp List Asp Asp Glu Pro Asp Asn Ile Ala 420 425 430 Ile Asp With Ile Ser Glu Glu Leu Asn Gly Val Asn Arg Asp Leu 435 440 445 Arg Gin Pro Tyr Asp Pro Thr Ala Pro Ile Glu Asp Gin Pro Gly Ile 450 455 460 Val Arg Thr Arg Ile Val Arg His Phe Asp Ser Ala Trp Glu Met With 465 470 475 480 Phe Glu Asn Ala Ile Ser Arg Ile Phe Leu Gly Val His Trp Arg Phe 485 490 495 Asp Ala Ala Ala Ala Arg Asp Ile Leu Ile Pro Thr Asn Thr Lys Asp 500 505 510 Val Tyr Ala Val Asp Ser Asn Gly Ala Thr Val Phe Gin Asn Val Glu 515 520 525 Asp Val Arg Tyr Ser Thr Lys Gly Thr Arg Glu Gly Arg Glu Gly Leu 530 535 540 Phe Pro Ile Gly Gly Val Pro Leu Gly Ile Glu Ile Ala Asp Glu Ile 545 550 555 560 Phe Asn Asn Gly Leu Arg Pro Thr Pro Pro Glu Leu Gin Pro With Pro 565 570 575 Gin Asp Thr Pro Val Gin List Pro Val Gin Gly With Trp Asp Glu Gin 580 585 590 Val Pro Leu Val List Glu Ala Pro 595 600 <210> 83 <211> 485 <212> PRT <213> Artificial <220> <223> Amylase enzymes <4 OO> 83 His His Asp Gly Thr Asn Gly Thr Ile With Gin Tyr Phe Glu Trp Asn 15 10 15 Val Pro Asn Asp Gly Gin His Trp Asn Arg Leu His Asn Asn Ala Gin 20 25 30 Asn Leu Lys Asn Ala Gly Ile Thr Ala Ile Trp Ile Pro Pro Ala Trp 35 40 45 Lys Gly Thr Ser Gin Asn Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr 50 55 60 Asp Leu Gly Glu Phe Asn Gin Lys Gly Thr Val Arg Thr Lys Tyr Gly 65 70 75 80 Thr Lys Ala Glu Leu Glu Arg Ala Ile Arg Ser Leu Lys Ala Asn Gly 85 90 95 Ile Gin Val Tyr Gly Asp Val Val With Asn His List Gly Gly Ala Asp 105 110 Phe Thr Glu Arg Val Gin Ala Val Glu Val Asn Pro Gin Asn Arg Asn H5 120 125 Gin Glu Val Ser Gly Thr Tyr Gin Ile Glu Ala Trp Thr Gly Phe Asn 130 135 140 Phe Pro Gly Arg Gly Asn Gin His Ser Ser Phe Lys Trp Arg Trp Tyr 145 150 155 160 His Phe Asp Gly Thr Asp Trp Asp Gin Ser Arg Gin Leu Asa Arg 165 170 175 Ile Tyr List Phe Arg Gly Asp Gly List Ala Trp Asp Trp Glu Val Asp 180 185 190 Thr Glu Asn Gly Asn Tyr Asp Tyr Leu With Tyr Ala Asp Val Asp Asp Met 195 200 205 Asp His Pro Glu Val Ile Asn Glu Leu Asn Arg Trp Gly Val Trp Tyr 210 215 220 Ala Asn Thr Leu Asn Leu Asp Gly Phe Arg Leu Asp Ala Val Lys His 225 230 235 240 Ile Lys Phe Ser Phe With Arg Asp Trp Leu Gly His Val Arg Gly Gin 245 250 255 Thr Gly Lys Asn Leu Phe Ala Val Ala Glu Tyr Trp Lys Asn Asp Leu 260 265 270 Gly Ala Leu Glu Asn Tyr Leu Ser Lys Thr Asn Trp Thr With Ser Ala 275 280 285 Phe Asp Val Pro Leu His Tyr Asn Leu Tyr Gin Ala Ser Asn Ser Ser 290 295 300 Gly Asn Tyr Asp With Arg Asn Leu Leu Asn Gly Thr Leu Val Gin Arg 305 310 315 320 His Pro Ser His Ala Val Thr Phe Val Asp Asn His Asp Thr Gin Pro 325 330 335 Gly Glu Ala Leu Glu Ser Phe Val Gin Gly Trp Phe List Pro Leu Ala 340 345 350 Tyr Ala Thr lie Leu Thr Arg Glu Gin Gly Tyr Pro Gin Val Phe Tyr 355 360 365 Gly Asp Tyr Tyr Gly lie Pro Ser Asp Gly Val Pro Ser Tyr Arg Gin 370 375 380 Gin lie Asp Pro Leu Leu Lys Ala Arg Gin Gin Tyr Ala Tyr Gly Arg 385 390 395 400 Gin His Asp Tyr Phe Asp His Trp Asp Val He Gly Trp Thr Arg Glu 405 410 415 Gly Asn Ala SerHis Pro Asn Ser Gly Leu Ala Thr lie With Ser Asp 420 425 430 Gly Pro Gly Gly Ser Lys Trp With Tyr Val Gly Arg Gin Lys Ala Gly 435 440 445 Glu Val Trp His Asp With Thr Gly Asn Arg Ser Gly Thr Val Thr Ile 450 455 460 Asn Gin Asp Gly Trp Gly His Phe Phe Val Asn Gly Gly Ser Val Ser 465 470 475 480 Val Trp Val Lys Arg 485 <210> 84 <211> 485 <212> PRT <213> Artificial <220> <223> Amylase protein <400> 84 His Asp Asp Gly Thr Asn Gly Thr Ile Met Gin Tyr Phe Glu Trp Asn 1 5 10 15 Val Pro Asn Asp Gly Gin His Trp Asn Arg Leu His Asn Asn Ala Gin 20 25 30 Asn Leu Lys Asn Ala Gly Ile Thr Ala Ile Trp Ile Pro Pro Ala Trp 35 40 45 Lys Gly Thr Ser Gin Asn Asp Val Gly Tyr Gly Ala Tyr Asp Leu Tyr 50 55 60 Asp Leu Gly Glu Phe Asn Gin Lys Gly Thr Val Arg Thr Lys Tyr Gly 65 70 75 80 Thr Lys Ala Glu Leu Glu Arg Ala Ile Arg Ser Leu Lys Ala Asn Gly 85 90 95 Ile Gin Val Tyr Gly Asp Val Val With Asn His List Gly Gly Ala Asp 100 105 110 Phe Thr Glu Arg Val Gin Ala Val Glu Val Asn Pro Gin Asn Arg Asn 115 120 125 Gin Glu Val Ser Gly Thr Tyr Gin Ile Glu Ala Trp Thr Gly Phe Asn 130 135 140 Phe Pro Gly Arg Gly Asn Gin His Ser Ser Phe Lys Trp Arg Trp Tyr 145 150 155 160 His Phe Asp Gly Thr Asp Trp Asp Gin Ser Arg Gin Leu Asa Arg 165 170 175 Ile Tyr List Phe Arg Gly Asp Gly List Ala Trp Asp Trp Glu Val Asp 180 185 190 Thr Glu Asn Gly Asn Tyr Asp Tyr Leu With Tyr Ala Asp Val Asp With 195 200 205 Asp His Pro G lu Val Ile Asn Glu Leu Asn Arg Trp Gly Val Trp Tyr 210 215 220 Ala Asn Thr Leu Asn Leu Asp Gly Phe Arg Leu Asp Ala Val Lys His 225 230 235 240 Ile Lys Phe Ser Phe With Arg Asp Trp Leu Gly His Val Arg Gly Gin 245 250 255 Thr Gly Lys Asn Leu Phe Ala Val Ala Glu Tyr Trp Lys Asn Asp Leu 260 265 270 Gly Ala Leu Glu Asn Tyr Leu Ser Lys Thr Asn Trp Thr With Ser Ala 275 280 285 Phe Asp Val Pro Leu His Tyr Asn Leu Tyr Gin Ala Ser Asn Ser Ser 290 295 300 Gly Asn Tyr Asp With Arg Asn Leu Leu Asn Gly Thr Leu Val Gin Arg 305 310 315 320 His Pro Ser His Ala Val Thr Phe Val Asp Asn His Asp Thr Gin Pro 325 330 335 Gly Glu Ala Leu Glu Ser Phe Val Gin Gly Trp Phe Lys Pro Leu Ala 340 345 350 Tyr Ala Thr Ile Leu Thr Arg Glu Gin Gly Tyr Pro Gin Val Phe Tyr 355 360 365 Gly Asp Tyr Tyr Gly Ile Pro Ser Asp Gly Val Pro Ser Tyr Arg Gin 370 375 380 Gin Ile Asp Pro Leu Leu Lys Ala Arg Gin Gin Tyr Ala Tyr Gly Thr 385 390 395 400 Gin His Asp Tyr Leu Asp Asn Gin Asp Val Ile Gly Trp Thr Arg Glu 405 410 415 Gly Asp Ser A la His Ala Gly Ser Gly Leu Ala Thr Val With Ser Asp 420 425 430 Gly Pro Gly Gly Ser Lys Thr With Tyr Val Gly Thr Ala His Ala Gly 435 440 445 Gin Val Phe Lys Asp Ile Thr Gly Asn Arg Thr Asp Thr Val Thr Ile 450 455 460 Asn Ser Ala Gly Asn Gly Thr Phe Pro Cys Asn Gly Gly Ser Val Ser 465 470 475 480 Ile Trp Val Lys Gin 485
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