DK2106447T3 - Fremgangsmåde til methanoluafhængig induktion fra methanolinducerbare promotorer i Pichia - Google Patents

Fremgangsmåde til methanoluafhængig induktion fra methanolinducerbare promotorer i Pichia

Info

Publication number
DK2106447T3
DK2106447T3 DK08701669.7T DK08701669T DK2106447T3 DK 2106447 T3 DK2106447 T3 DK 2106447T3 DK 08701669 T DK08701669 T DK 08701669T DK 2106447 T3 DK2106447 T3 DK 2106447T3
Authority
DK
Denmark
Prior art keywords
methanol
pichia
inducible promoters
independent induction
induction
Prior art date
Application number
DK08701669.7T
Other languages
English (en)
Inventor
Noriko Tsutsumi
Yuji Terui
Shinobu Takagi
Xiangyu Kong
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of DK2106447T3 publication Critical patent/DK2106447T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK08701669.7T 2007-01-26 2008-01-25 Fremgangsmåde til methanoluafhængig induktion fra methanolinducerbare promotorer i Pichia DK2106447T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200700122 2007-01-26
PCT/EP2008/050870 WO2008090211A1 (en) 2007-01-26 2008-01-25 Method for methanol independent induction from methanol inducible promoters in pichia

Publications (1)

Publication Number Publication Date
DK2106447T3 true DK2106447T3 (da) 2013-06-24

Family

ID=38157821

Family Applications (1)

Application Number Title Priority Date Filing Date
DK08701669.7T DK2106447T3 (da) 2007-01-26 2008-01-25 Fremgangsmåde til methanoluafhængig induktion fra methanolinducerbare promotorer i Pichia

Country Status (6)

Country Link
US (2) US8143023B2 (da)
EP (1) EP2106447B1 (da)
JP (1) JP5290997B2 (da)
CN (1) CN101589146A (da)
DK (1) DK2106447T3 (da)
WO (1) WO2008090211A1 (da)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2060632A1 (en) * 2007-10-29 2009-05-20 Technische Universität Berlin Method of modifying a yeast cell for the production of ethanol
WO2010053636A1 (en) * 2008-11-06 2010-05-14 Tate & Lyle Technology Ltd Enhanced production and purification of a natural high intensity sweetener
CN102757976B (zh) 2012-07-12 2014-10-08 华东理工大学 消除甲醇诱导型启动子单一甲醇碳源依赖性的方法
PL2964765T3 (pl) 2013-03-08 2019-10-31 Keck Graduate Inst Of Applied Life Sciences Promotory drożdżowe z pichia pastoris
ES2961268T3 (es) * 2013-03-08 2024-03-11 Biogrammatics Inc Promotores de levadura para la expresión de proteínas
KR101464437B1 (ko) 2013-06-19 2014-11-27 한국생명공학연구원 재조합 단백질의 효율적인 분비를 위한 코돈 최적화
LT3294762T (lt) 2015-05-11 2022-04-11 Impossible Foods Inc. Metilotrofinių mielių genų inžinerijos ekspresijos konstruktai ir būdai
ES2740449T3 (es) * 2015-12-22 2020-02-05 Bisy E U Célula de levadura
CN108949869B (zh) * 2017-05-18 2022-12-02 华东理工大学 无碳源阻遏毕赤酵母表达系统、其建立方法及应用
WO2019236452A1 (en) * 2018-06-07 2019-12-12 Basf Se Methods for identifying promoters for protein production in yeast
CN109749948A (zh) * 2019-03-05 2019-05-14 天津科技大学 一种利用tef1启动子调控黏玉米谷氨酰胺转氨酶表达的重组毕赤酵母菌株及构建方法
CN110029120B (zh) * 2019-03-19 2022-03-01 青岛蔚蓝生物集团有限公司 一种植酸酶高产菌株及其应用
CN110029122B (zh) * 2019-03-19 2022-03-01 青岛蔚蓝生物集团有限公司 一种甘露聚糖酶高产菌株及其应用
CN114555812A (zh) 2019-04-17 2022-05-27 非凡食品有限公司 用于蛋白质产生的材料和方法
CA3137581A1 (en) 2019-04-25 2020-10-29 Impossible Foods Inc. Strains and methods for production of heme-containing proteins
EP3922726A1 (en) * 2020-06-10 2021-12-15 Erber Aktiengesellschaft Truncated promoter for recombinant gene expression
WO2022130163A1 (en) * 2020-12-14 2022-06-23 Indian Institute Of Science Nucleotide sequence, expression vectors, recombinant yeast cells and applications thereof
CN115851809A (zh) * 2022-12-01 2023-03-28 江苏亢钧生物科技有限公司 一种腐生子囊菌抗菌肽Plectasin的基因工程制备方法
CN118308360B (zh) * 2024-06-07 2024-08-16 中国人民解放军军事科学院军事医学研究院 驱动基因在酵母中表达人血清白蛋白的启动子与应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3789866T2 (de) 1987-07-17 1994-09-22 Rhein Biotech Gesellschaft Fuer Neue Biotechnologische Prozesse Und Produkte Mbh, 40595 Duesseldorf DNA-Moleküle, die für FMDH-Kontrollabschnitte und Strukturgene für ein Protein mit FMDH-Aktivität kodieren, sowie deren Anwendung.
CA1324969C (en) * 1988-05-06 1993-12-07 Jeffrey R. Shuster High level expression of proteins in yeast
AU4332589A (en) * 1988-09-26 1990-04-18 Salk Institute Biotechnology/Industrial Associates, Inc., The Mixed feed recombinant yeast fermentation
EP0911416B1 (en) 1997-10-01 2006-05-17 DSM IP Assets B.V. Protein production process
WO2000056903A2 (en) 1999-03-22 2000-09-28 Zymogenetics, Inc. IMPROVED METHODS FOR PRODUCING PROTEINS IN TRANSFORMED $i(PICHIA)
ITMI20011728A1 (it) 2001-08-06 2003-02-06 Consorzio Per Le Ricerche E Lo Vettore per l'integrazione sito-specifica di sequenze di dna eterologhe in lieviti metilotrofi
DE10220894A1 (de) * 2002-05-10 2003-11-27 Rhein Biotech Proz & Prod Gmbh Promotoren mit veränderter Transkriptionseffizienz

Also Published As

Publication number Publication date
JP5290997B2 (ja) 2013-09-18
EP2106447A1 (en) 2009-10-07
US8236528B2 (en) 2012-08-07
CN101589146A (zh) 2009-11-25
EP2106447B1 (en) 2013-04-17
WO2008090211A1 (en) 2008-07-31
US8143023B2 (en) 2012-03-27
JP2010516268A (ja) 2010-05-20
US20120142053A1 (en) 2012-06-07
US20090311749A1 (en) 2009-12-17

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