DK2195457T3 - Enkeltcellebaseret reporterassay til overvågning afgenekspressionsmønstre med høj spatiotemporal opløsning - Google Patents

Enkeltcellebaseret reporterassay til overvågning afgenekspressionsmønstre med høj spatiotemporal opløsning

Info

Publication number
DK2195457T3
DK2195457T3 DK08804165.2T DK08804165T DK2195457T3 DK 2195457 T3 DK2195457 T3 DK 2195457T3 DK 08804165 T DK08804165 T DK 08804165T DK 2195457 T3 DK2195457 T3 DK 2195457T3
Authority
DK
Denmark
Prior art keywords
gene expression
expression patterns
reporter assay
monitoring high
cell reporter
Prior art date
Application number
DK08804165.2T
Other languages
English (en)
Inventor
Jost Enninga
Philippe Sansonetti
Musa Mhlanga
Ulf Nehrbass
Original Assignee
Pasteur Institut
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pasteur Institut filed Critical Pasteur Institut
Application granted granted Critical
Publication of DK2195457T3 publication Critical patent/DK2195457T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK08804165.2T 2007-09-12 2008-09-12 Enkeltcellebaseret reporterassay til overvågning afgenekspressionsmønstre med høj spatiotemporal opløsning DK2195457T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP07291093A EP2036989B1 (en) 2007-09-12 2007-09-12 Polynucleotide suitable for single cell based reporter assay to monitor gene expression patterns with high spatio-temporal resolution
PCT/EP2008/062204 WO2009065635A1 (en) 2007-09-12 2008-09-12 Single cell based reporter assay to monitor gene expression patterns with high spatio-temporal resolution

Publications (1)

Publication Number Publication Date
DK2195457T3 true DK2195457T3 (da) 2012-10-29

Family

ID=38962162

Family Applications (2)

Application Number Title Priority Date Filing Date
DK07291093.8T DK2036989T3 (da) 2007-09-12 2007-09-12 Polynukleotid, der er egnet til enkeltcellebaseret reporterassay tilovervågning af genekspressionsmønstre med høj spatiotemporalopløsning
DK08804165.2T DK2195457T3 (da) 2007-09-12 2008-09-12 Enkeltcellebaseret reporterassay til overvågning afgenekspressionsmønstre med høj spatiotemporal opløsning

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK07291093.8T DK2036989T3 (da) 2007-09-12 2007-09-12 Polynukleotid, der er egnet til enkeltcellebaseret reporterassay tilovervågning af genekspressionsmønstre med høj spatiotemporalopløsning

Country Status (9)

Country Link
US (1) US9663833B2 (da)
EP (2) EP2036989B1 (da)
JP (1) JP2010538628A (da)
AU (1) AU2008328177B2 (da)
CA (1) CA2699358C (da)
DK (2) DK2036989T3 (da)
ES (2) ES2391906T3 (da)
PL (2) PL2036989T3 (da)
WO (1) WO2009065635A1 (da)

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
WO2012135730A2 (en) * 2011-03-30 2012-10-04 Verinata Health, Inc. Method for verifying bioassay samples
WO2012162260A2 (en) 2011-05-21 2012-11-29 Kdt, Llc. Transgenic biosensors
CA2865575C (en) 2012-02-27 2024-01-16 Cellular Research, Inc. Compositions and kits for molecular counting
WO2014144495A1 (en) 2013-03-15 2014-09-18 Abvitro, Inc. Single cell bar-coding for antibody discovery
US9567645B2 (en) 2013-08-28 2017-02-14 Cellular Research, Inc. Massively parallel single cell analysis
TWI493036B (zh) * 2013-12-24 2015-07-21 Ind Tech Res Inst 含一cd54表現之報導系統之免疫細胞的應用以及一經選殖之新穎細胞
EP3089822B1 (en) * 2013-12-30 2022-04-06 Atreca, Inc. Analysis of nucleic acids associated with single cells using nucleic acid barcodes
KR102541849B1 (ko) 2014-09-15 2023-06-09 에이비비트로, 엘엘씨 고-처리량 뉴클레오티드 라이브러리 서열결정
US10830639B2 (en) 2014-09-25 2020-11-10 Northwestern University Devices, methods, and systems relating to super resolution imaging
US11574368B1 (en) 2014-10-06 2023-02-07 State Farm Mutual Automobile Insurance Company Risk mitigation for affinity groupings
US20210166320A1 (en) 2014-10-06 2021-06-03 State Farm Mutual Automobile Insurance Company System and method for obtaining and/or maintaining insurance coverage
US10817949B1 (en) 2014-10-06 2020-10-27 State Farm Mutual Automobile Insurance Company Medical diagnostic-initiated insurance offering
US10713728B1 (en) 2014-10-06 2020-07-14 State Farm Mutual Automobile Insurance Company Risk mitigation for affinity groupings
US10664920B1 (en) 2014-10-06 2020-05-26 State Farm Mutual Automobile Insurance Company Blockchain systems and methods for providing insurance coverage to affinity groups
CN107208158B (zh) 2015-02-27 2022-01-28 贝克顿迪金森公司 空间上可寻址的分子条形编码
WO2016149422A1 (en) * 2015-03-16 2016-09-22 The Broad Institute, Inc. Encoding of dna vector identity via iterative hybridization detection of a barcode transcript
EP3835431B1 (en) 2015-03-30 2022-11-02 Becton, Dickinson and Company Methods for combinatorial barcoding
US11390914B2 (en) 2015-04-23 2022-07-19 Becton, Dickinson And Company Methods and compositions for whole transcriptome amplification
JP2016208851A (ja) * 2015-04-30 2016-12-15 国立大学法人東北大学 Tlr刺激物質の検出方法
JP6940484B2 (ja) 2015-09-11 2021-09-29 セルラー リサーチ, インコーポレイテッド ライブラリー正規化のための方法および組成物
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
AU2017331459B2 (en) * 2016-09-26 2023-04-13 Becton, Dickinson And Company Measurement of protein expression using reagents with barcoded oligonucleotide sequences
KR102476709B1 (ko) 2016-11-21 2022-12-09 나노스트링 테크놀로지스, 인크. 화학적 조성물 및 이것을 사용하는 방법
CN110382708A (zh) 2017-02-01 2019-10-25 赛卢拉研究公司 使用阻断性寡核苷酸进行选择性扩增
WO2018226293A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
EP3759117A1 (en) * 2018-03-02 2021-01-06 Life Technologies Corporation Novel quencher and reporter dye combinations
EP3788170B1 (en) 2018-05-03 2025-01-01 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
EP3788171B1 (en) 2018-05-03 2023-04-05 Becton, Dickinson and Company High throughput multiomics sample analysis
WO2019222284A1 (en) * 2018-05-14 2019-11-21 The Broad Institute, Inc. In situ cell screening methods and systems
JP7525402B2 (ja) 2018-05-14 2024-07-30 ナノストリング テクノロジーズ,インコーポレイティド 化学的組成物とそれを利用する方法
WO2020046809A1 (en) * 2018-08-27 2020-03-05 The Regents Of The University Of California Reporter nucleic acids for type v crispr-mediated detection
WO2020063632A1 (en) * 2018-09-25 2020-04-02 Yichang Humanwell Pharmaceutical Co., Ltd Regulator of TGR5 Signaling as Immunomodulatory Agent
WO2020072380A1 (en) 2018-10-01 2020-04-09 Cellular Research, Inc. Determining 5' transcript sequences
EP3877520B1 (en) 2018-11-08 2025-03-19 Becton, Dickson And Company Whole transcriptome analysis of single cells using random priming
EP4745247A2 (en) 2018-12-13 2026-05-20 Becton Dickinson Co SELECTIVE EXTENSION IN A SINGLE-CELL WHOLE-TRANSCRIPTOME ANALYSIS
CN113574178B (zh) 2019-01-23 2024-10-29 贝克顿迪金森公司 与抗体关联的寡核苷酸
CN120099139A (zh) 2019-02-14 2025-06-06 贝克顿迪金森公司 杂合体靶向和全转录物组扩增
US11965208B2 (en) 2019-04-19 2024-04-23 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
CN120099137A (zh) 2019-07-22 2025-06-06 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
EP4055160B1 (en) 2019-11-08 2024-04-10 Becton Dickinson and Company Using random priming to obtain full-length v(d)j information for immune repertoire sequencing
EP4090763B1 (en) 2020-01-13 2024-12-04 Becton Dickinson and Company Methods and compositions for quantitation of proteins and rna
WO2021146219A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Cell capture using du-containing oligonucleotides
ES2993319T3 (en) 2020-01-29 2024-12-27 Becton Dickinson Co Barcoded wells for spatial mapping of single cells through sequencing
CN115151810A (zh) 2020-02-25 2022-10-04 贝克顿迪金森公司 实现使用单细胞样品作为单色补偿对照的双特异性探针
US11661625B2 (en) 2020-05-14 2023-05-30 Becton, Dickinson And Company Primers for immune repertoire profiling
CN115803445A (zh) 2020-06-02 2023-03-14 贝克顿迪金森公司 用于5撇基因表达测定的寡核苷酸和珠
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
AU2021401791A1 (en) * 2020-12-15 2023-07-06 Quantum-Si Incorporated Molecular barcode analysis by single-molecule kinetics
CN115479923B (zh) * 2021-05-31 2024-06-18 北京大学 一种基于荧光共振能量转移的活细胞单分子rna标记方法
CA3255881A1 (en) 2022-06-17 2023-12-21 Insitro, Inc. In situ sequencing of RNA transcripts with non-uniform 5' ends

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999055886A1 (en) * 1998-04-24 1999-11-04 Genova Pharmaceuticals Corporation Function-based gene discovery
US6203986B1 (en) * 1998-10-22 2001-03-20 Robert H. Singer Visualization of RNA in living cells
US6692965B1 (en) * 1999-11-23 2004-02-17 Chromocell Corporation Isolation of living cells and preparation of cell lines based on detection and quantification of preselected cellular ribonucleic acid sequences
AU2001245945A1 (en) * 2000-03-23 2001-10-03 Pe Corporation (Ny) Detection kits, such as nucleic acid arrays, for detecting the expression of 10,000 or more drosophila genes and uses thereof
WO2004027028A2 (en) * 2002-09-18 2004-04-01 The Trustees Of The University Of Pennsylvania Compositions, methods and kits for detection of an antigen on a cell and in a biological mixture
US7829278B2 (en) * 2004-10-28 2010-11-09 Board Of Trustees Of The University Of Illinois Polynucleotide barcoding

Also Published As

Publication number Publication date
US9663833B2 (en) 2017-05-30
PL2195457T3 (pl) 2012-12-31
PL2036989T3 (pl) 2012-12-31
ES2391906T3 (es) 2012-11-30
ES2392005T3 (es) 2012-12-03
EP2036989A1 (en) 2009-03-18
EP2036989B1 (en) 2012-07-25
AU2008328177A1 (en) 2009-05-28
EP2195457B1 (en) 2012-07-25
CA2699358C (en) 2017-11-21
EP2195457A1 (en) 2010-06-16
WO2009065635A1 (en) 2009-05-28
US20110021369A1 (en) 2011-01-27
DK2036989T3 (da) 2012-10-22
AU2008328177B2 (en) 2015-01-22
JP2010538628A (ja) 2010-12-16
CA2699358A1 (en) 2009-05-28

Similar Documents

Publication Publication Date Title
DK2036989T3 (da) Polynukleotid, der er egnet til enkeltcellebaseret reporterassay tilovervågning af genekspressionsmønstre med høj spatiotemporalopløsning
DK2497834T4 (da) Fremgangsmåde til påvisning af methyleringen af colon-cancer-specifikke methyleringsmarkørgener til colon-cancer-diagnose
DK2129797T3 (da) Nukleinsyrepåvisning
EP2139616A4 (en) NEW ELECTRODE
BRPI0811503A2 (pt) Diagnótico de melanoma por análise de ácido nucleico
DE602007003881D1 (de) Intraperikardiale Elektrode
DE602008005237D1 (de) Tintenstrahlaufzeichnungsverfahren
DK2344682T3 (da) Elektrode for elektrolysecelle
DK2061766T3 (da) Nye forbindelser
SMT201600131B (it) Procedimento per la produzione di piastrine
EP2344029A4 (en) Electrode system
BRPI0817781A2 (pt) piperazino-diidroteinopirimidinas substituídas com heterociclo
EP2519522A4 (en) SUBSTITUTED IMIDAZOPYRIDINYL-AMINOPYRIDINE COMPOUNDS
DK2250161T3 (da) Forbindelser med CRTH2-antagonistaktivitet
EP2114634A4 (en) TESTING HEAD MANIPULATOR
BRPI0921710A2 (pt) relatório de medição de células vizinhas
DK3564240T3 (da) Piperidinmellemprodukter
DK2120936T3 (da) Nye fremgangsmåder
DK2234976T3 (da) Nye pyrazol-substituerede arylamider
DK1991541T3 (da) Nye pyron-indolderivater samt fremgangsmåde til deres fremstilling
DK2074123T3 (da) Nye anxiolytiske forbindelser
EP2117306A4 (en) NEUROGENIC COMPOUNDS
EP2266301A4 (en) ACTIVITY MESSAGE FOR CUTTING APPLICATIONS
EP2514426A4 (en) CONNECTION WITH ANTI-INFLAMMATORY EFFECT
EP2192402A4 (en) Enzyme electrode