DK2201025T3 - Funktionelle lipidkonstruktionsprodukter - Google Patents
Funktionelle lipidkonstruktionsprodukter Download PDFInfo
- Publication number
- DK2201025T3 DK2201025T3 DK08836952.5T DK08836952T DK2201025T3 DK 2201025 T3 DK2201025 T3 DK 2201025T3 DK 08836952 T DK08836952 T DK 08836952T DK 2201025 T3 DK2201025 T3 DK 2201025T3
- Authority
- DK
- Denmark
- Prior art keywords
- cells
- peptide
- modified
- construct
- lipid
- Prior art date
Links
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Classifications
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- C07K7/02—Linear peptides containing at least one abnormal peptide link
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- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
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- C07F9/08—Esters of oxyacids of phosphorus
- C07F9/09—Esters of phosphoric acids
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- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
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- C07F9/553—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having one nitrogen atom as the only ring hetero atom
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
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- C07F9/6561—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing systems of two or more relevant hetero rings condensed among themselves or condensed with a common carbocyclic ring or ring system, with or without other non-condensed hetero rings
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
- C07K5/02—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing at least one abnormal peptide link
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
Landscapes
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Claims (17)
1. Funktionelt lipidkonstruktionsprodukt med strukturen F-S-L, hvor F er en funktionel del; L er phosphatidylethanolamin; S er en afstandsdannende gruppe, som kovalent forbinder F til L; og strukturen af det funktionelle lipidkonstruktionsprodukt omfatter den partielle struktur:
hvor M er Fl eller CH3; g er heltallet 1, 2 eller 3; h er heltallet 1, 2, 3 eller 4; v er heltallet 3, 4 eller 5; M' er en monovalent kation; * er forskellig fra Fl; og Ri og R2 er uafhængigt udvalgt fra gruppen bestående af alkyl- eller alkenylsubstituenter af fedtsyrerne trans-3-hexadecensyre, cis-5-hexadecensyre, cis-7-hexadecensyre, cis-9-hexadecensyre, cis-6-oktadecensyre, cis-9-oktadecensyre, frans-9-oktadecensyre, trans-ll-oktadecensyre, c/'s-11-oktadecensyre, cis- 11-eicosensyre eller c/s-13-docosensyre.
2. Funktionelt lipidkonstruktionsprodukt ifølge krav 1 med strukturen:
hvor F er et kulhydrat; x er heltallet 2, 3 eller 4; y er heltallet 1, 2 eller 3; og R3 er O i en substitueret hydroxylgruppe i kulhydratet.
3. Funktionelt lipidkonstruktionsprodukt ifølge krav 1 med strukturen: i
hvor F er den kemisk reaktive gruppe maleimid, og w er heltallet 1 eller 2.
4. Funktionelt lipidkonstruktionsprodukt ifølge krav 1 med strukturen:
hvor F er konjugatoren biotin, og k er heltallet 2, 3 eller 4.
5. Funktionelt lipidkonstruktionsprodukt ifølge krav 1 med strukturen:
hvor F er fluorophoren i fluorescein (eller et af dets derivater), z er heltallet 3, 4 eller 5, og R3 er C i thiocyanatsubstituenten i isothiocyanatderivatet i fluorescein (eller et af dets derivater).
6. Funktionelt lipidkonstruktionsprodukt ifølge krav 1 med strukturen:
hvor F er et peptid; w er heltallet 1 eller 2; og R3 er S i en substitueret sulfhydrylgruppe i en Cys-rest i peptidet.
7. Funktionelt lipidkonstruktionsprodukt ifølge et hvilket som helst af kravene 1 til 6, hvor L er l,2-0-dioleoyl-sn-glycero-3-phosphatidylethanolamin (DOPE) eller 1,2-0-distearyl-sn-glycero-3-phosphatidylethanolamin (DSPE).
8. Fremgangsmåde til påvisning af reaktivt antistof i serummet hos et individ, hvilken fremgangsmåde omfatter følgende trin: • at bringe en prøve af serummet i kontakt med en opslæmning af røde blodlegemer, der er modificeret til at inkorporere et funktionelt lipidkonstruktionsprodukt ifølge et af kravene 2 eller 6, til tilvejebringelse af en blanding; • at inkubere blandingen i et tidsrum og ved en temperatur, der er tilstrækkelig til at tillade agglutinering; og • at bestemme graden af agglutinering af cellerne i blandingen; hvor F er et kulhydrat eller et peptid omfattende en epitop til det reaktive antistof.
9. Fremgangsmåde ifølge krav 8, hvor fremgangsmåden omfatter det mellemliggende trin at tilsætte et antiindividglubolinantistof til blandingen forud for bestemmelse af graden af agglutinering af cellerne i blandingen.
10. Fremgangsmåde ifølge krav 9, hvor antiindividglobulinantistoffet er antihuman globulin(AFIG)antistof.
11. Fremgangsmåde ifølge et hvilket som helst af kravene 8 til 10, hvor F er et peptid, og det reaktive antistof er reaktivt over for et antistof udvalgt fra gruppen bestående af: Glycophorin A, Glycophorin B eller mutationer deraf (herunder MNS-blodgruppesystemet).
12. Fremgangsmåde til fremstilling af et konstruktionsprodukt ifølge krav 6, indbefattende trinnet at omsætte et peptid indbefattende en Cys-rest med et konstruktionsprodukt ifølge krav 3.
13. In v/'tro-fremgangsmåde til afstedkommelse af kvalitative og kvantitative ændringer i de funktionelle dele udtrykt ved overfladen af en celle eller en multicellulær struktur, hvilken fremgangsmåde indbefatter trinnet at bringe cellen eller den multicellulære struktur i kontakt med en opløsning af et funktionelt lipidkonstruktionsprodukt ifølge et hvilket som helst af kravene 1 til 7 i et tidsrum og ved en temperatur, der er tilstrækkelig til at tillade konstruktionsproduktet at inkorporeres i cellen eller den multicellulære struktur, hvor den multicellulære struktur ikke er et humant embryon.
14. In vitro-fremgangsmåde til immobilisering af en eller flere celler eller multicellulære strukturer, hvilken fremgangsmåde indbefatter trinnene: • at bringe cellerne eller de multicellulære strukturer i kontakt med en opløsning af konstruktionsprodukter ifølge krav 4 i et tidsrum og ved en temperatur, der er tilstrækkelig til at tillade en virksom mængde konstruktionsprodukter at inkorporeres i cellerne eller de multicellulære strukturer til tilvejebringelse af modificerede celler eller multicellulære strukturer; og • at bringe de modificerede celler eller multicellulære strukturer i kontakt med et avi-din-beklædt substrat, som er i stand til reversibelt at blive lokaliseret til en overflade, hvor den multicellulære struktur ikke indbefatter et humant embryon.
15. Fremgangsmåde ifølge krav 14, hvor det avidin-beklædte substrat er avidin-beklædte magnetiske perler.
16. Fremgangsmåde ifølge krav 15, hvor de avidin-beklædte magnetiske perler er i stand til reversibelt at blive lokaliseret til en overflade ved påføring af et magnetfelt.
17. In v/fro-fremgangsmåde til at fremme aggregeringen af en første og en anden population af celler, hvilken fremgangsmåde indbefatter følgende trin: • at bringe den første population af celler i kontakt med en opløsning af konstruktionsprodukter ifølge krav 4 i et tidsrum og ved en temperatur, der er tilstrækkelig til at tillade en virksom mængde af konstruktionsprodukterne at inkorporeres i cellerne til tilvejebringelse af modificerede celler af den første population; • at bringe den anden population af celler i kontakt med en opløsning af konstruktionsprodukter ifølge krav 4 i et tidsrum og ved en temperatur, der er tilstrækkelig til at tillade en virksom mængde af konstruktionsprodukterne at inkorporeres i cellerne til tilvejebringelse af modificerede celler af den anden population; • at bringe de modificerede celler af en af populationerne i kontakt med et overskud af avidin; og • at bringe de modificerede celler af den første og den anden population i kontakt med hinanden.
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| PCT/NZ2008/000266 WO2009048343A1 (en) | 2007-10-12 | 2008-10-13 | Functional lipid constructs |
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| US10919941B2 (en) | 2007-10-12 | 2021-02-16 | Kode Biotech Limited | Functional lipid constructs |
| WO2010143983A1 (en) * | 2009-06-12 | 2010-12-16 | Kode Biotech Limited | Assays for serological detection of syphilis |
| US10408717B2 (en) | 2009-06-29 | 2019-09-10 | Nicolai Vladimirovich Bovin | Printing of FSL constructs |
| WO2011002310A1 (en) | 2009-06-29 | 2011-01-06 | Nicolai Vladimirovich Bovin | Printing of fsl constructs |
| NZ591047A (en) | 2011-02-09 | 2013-11-29 | Vladimirovich Bovin Nicolai | In vivo methods of monitoring biodistribution |
| NZ591514A (en) * | 2011-03-03 | 2013-11-29 | Kode Biotech Ltd | Assay method |
| WO2013081471A2 (en) * | 2011-08-31 | 2013-06-06 | Nikolai Vladimirovich Bovin | Facile laboratory method for localising biomolecules to the surface of cells and viruses |
| US11073451B2 (en) | 2011-12-19 | 2021-07-27 | Kode Biotech Limited | Biocompatible method of functionalising substrates with inert surfaces |
| WO2015084187A1 (en) | 2013-12-02 | 2015-06-11 | Nicolai Bovin | Functionalizing nanofibres |
| US10457706B2 (en) * | 2014-11-21 | 2019-10-29 | Stephen Micheal Henry | Multivalent ligand-lipid constructs |
| EP3374367A1 (en) * | 2015-11-11 | 2018-09-19 | Agalimmune Limited | Glycolipid compounds and their uses in the treatment of tumours |
| EP3559666A1 (en) * | 2016-12-21 | 2019-10-30 | H. Hoffnabb-La Roche Ag | Assay for determining antibody or ligand binding and function |
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| CN108546677A (zh) * | 2018-04-25 | 2018-09-18 | 段莉 | 一种靶向孕妇血液中胎儿脱落细胞的功能红细胞 |
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| US5149794A (en) * | 1990-11-01 | 1992-09-22 | State Of Oregon | Covalent lipid-drug conjugates for drug targeting |
| US6676946B2 (en) | 1997-03-27 | 2004-01-13 | Institut Pasteur | Multiple antigen glycopeptide carbohydrate vaccine comprising the same and use thereof |
| EP1007541B1 (en) | 1997-08-28 | 2006-08-23 | Biomira, Inc. | Randomly generated glycopeptide combinatorial libraries |
| US20030229013A1 (en) * | 2001-12-07 | 2003-12-11 | Shih-Kwang Wu | Solid phase method for synthesis peptide-spacer-lipid conjugates, conjugates synthesized thereby and targeted liposomes containing the same |
| AU2005223715A1 (en) | 2004-03-22 | 2005-09-29 | Kode Biotech Limited | Synthetic membrane anchors |
| JP5580202B2 (ja) * | 2007-10-12 | 2014-08-27 | コード バイオテック リミテッド | 機能性脂質構築物 |
| WO2011002310A1 (en) * | 2009-06-29 | 2011-01-06 | Nicolai Vladimirovich Bovin | Printing of fsl constructs |
| NZ591047A (en) * | 2011-02-09 | 2013-11-29 | Vladimirovich Bovin Nicolai | In vivo methods of monitoring biodistribution |
| WO2013081471A2 (en) * | 2011-08-31 | 2013-06-06 | Nikolai Vladimirovich Bovin | Facile laboratory method for localising biomolecules to the surface of cells and viruses |
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| US20120021430A1 (en) | 2012-01-26 |
| CA2702470C (en) | 2013-06-04 |
| CN101970460B (zh) | 2014-09-24 |
| WO2009048343A1 (en) | 2009-04-16 |
| US8669084B2 (en) | 2014-03-11 |
| JP2011501747A (ja) | 2011-01-13 |
| US9802981B2 (en) | 2017-10-31 |
| EP2201025A1 (en) | 2010-06-30 |
| CN101970460A (zh) | 2011-02-09 |
| EP2201025B1 (en) | 2016-04-20 |
| NZ584559A (en) | 2012-06-29 |
| JP5580202B2 (ja) | 2014-08-27 |
| EP2201025A4 (en) | 2013-04-24 |
| CA2702470A1 (en) | 2009-04-16 |
| AU2008311480A1 (en) | 2009-04-16 |
| US20140350217A1 (en) | 2014-11-27 |
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