DK2201120T3 - GCSF-fusionsproteinsystemer, der er egnet til høj ekspression af peptider - Google Patents
GCSF-fusionsproteinsystemer, der er egnet til høj ekspression af peptider Download PDFInfo
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- DK2201120T3 DK2201120T3 DK08852235.4T DK08852235T DK2201120T3 DK 2201120 T3 DK2201120 T3 DK 2201120T3 DK 08852235 T DK08852235 T DK 08852235T DK 2201120 T3 DK2201120 T3 DK 2201120T3
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- DK
- Denmark
- Prior art keywords
- peptide
- fusion
- use according
- gly
- gcsf
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
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- A61P3/00—Drugs for disorders of the metabolism
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/53—Colony-stimulating factor [CSF]
- C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
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- C07K14/575—Hormones
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- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/635—Parathyroid hormone, i.e. parathormone; Parathyroid hormone-related peptides
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- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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Claims (28)
1. Anvendelse af granulocytkolonistimulerende faktor (GCSF) til forøget ekspression af et peptid af interesse, hvor GCSF’en fusioneres med peptidet af interesse til at bevirke ekspression af dette i en E. coli-værtscelle.
2. Anvendelse ifølge krav 1, hvor GCSF’en danner et fusionsprodukt med peptidet.
3. Anvendelse ifølge krav 1 eller 2, hvor fusionsproduktkonstruktionen er repræsenteret af formlen fusionspartner-CS-fusionspeptid, hvor fusionspeptidet er et peptid af interesse, CS er et egnet spaltningssted, og fusionspartneren er GCSF.
4. Anvendelse ifølge krav 3, hvor CS enten findes i fusionspartneren eller fu-sionspeptidaminosyresekvenserne eller er en egnet linker.
5. Anvendelse ifølge et hvilket som helst af krav 2 til 4, som efter ekspressionen endvidere omfatter at isolere fusionsproduktet og at fraspalte GCSF-delen af fusionsproduktet til at give det ønskede peptid af interesse.
6. Anvendelse ifølge et hvilket som helst af de foregående krav, hvor fusionspartneren ved sin C-terminale ende er koblet med et peptid ved dettes N-termi-nale ende via et spaltningssted.
7. Anvendelse ifølge et hvilket som helst af de foregående krav, hvor peptidet er valgt blandt de peptider, der har en længde på fra 10 aminosyrer til 90 aminosyrer.
8. Anvendelse ifølge et hvilket som helst af de foregående krav, hvor peptidet er valgt blandt gruppen bestående af caltrin, calcitonin, insulin, angiotensin, vævsplasminogenaktivator, væksthormon, vækstfaktorer, væksthormonfrigi- vende faktorer, cytokiner, erythropoietin, interferoner, interleukiner, oxytocin, vasopressin, ACTH, kollagenbindende protein, parathyreoideahormon, glucagon-lignende peptid, glucagon, proinsulin, tumornekrosefaktor, stof P, hjernenatriure-tisk peptid, individuelle tunge og lette antistofkæder, peptidantibiotika, fuzeon, octreotid, somatostatin og egnede varianter af disse peptider.
9. Anvendelse ifølge et hvilket som helst af de foregående krav, hvor fusionsproduktkonstruktionen er klonet i en egnet ekspressionsvektor.
10. Anvendelse ifølge krav 9, hvor fusionsproduktkonstruktionen kodes i en egnet ekspressionsvektor, som er valgt blandt dem, der kan udtrykke fusionsproduktet i E. coli.
11. Anvendelse ifølge et hvilket som helst af de foregående krav, hvor peptidet er valgt blandt parathyreoideahormon (PTH) (1-34), angiotensin og proinsulin.
12. Anvendelse ifølge et hvilket som helst af de foregående krav, hvor peptidet er PTH (1 -34) af sekvens ID No 6 og sekvens ID No 7.
13. Anvendelse ifølge krav 11 eller 12, hvor peptidet PTH (1-34) udtrykkes af ekspressionsvektor pET27B-GCSF-PTH, som er deponeret under MTCC tilgangsnummer 5425.
14. Anvendelse ifølge et hvilket som helst af krav 1 til 11, hvor peptidet er proinsulin af sekvens ID No 12 og 13.
15. Anvendelse ifølge krav 14, hvor peptidet proinsulin udtrykkes af ekspressionsvektor pET27B-GCSF-proinsulin, som er deponeret under MTCC tilgangsnummer 5424.
16. Anvendelse ifølge et hvilket som helst af krav 1 til 11, hvor peptidet er an- giotensin af sekvens ID No 16 og 17.
17. Anvendelse ifølge krav 16, hvor peptidet angiotensin udtrykkes af ekspressionsvektor pET27B-GCSF-angiotensin, som er ekspressionsvektoren pET27B, i hvilken der er indført den GCSF-angiotensin-fusionsproduktkodende sekvens, som er angivet i sekvens ID No 16 og 17.
18. Anvendelse ifølge et hvilket som helst af krav 1 til 17, hvor spaltningsstedet er et enzymatisk eller kemisk spaltningssted.
19. Anvendelse ifølge krav 18, hvor spaltningsstedet er et kemisk spaltningssted.
20. Anvendelse ifølge krav 18, hvor spaltningsstedet er et enzymatisk spaltningssted.
21. Anvendelse ifølge krav 18 eller 19, hvor spaltningsstederne er de, som kan spaltes af kemikalier, der er valgt blandt cynogenbromid, 2-(2-nitrophenyl-sulphenyl)-methyl-3’-bromindolenin, BNPS-skatol, N-bromsuccinamid, O-iodos-benzoesyre, HBr/DMSO, NTCB, natriummetal i flydende ammoniak, hydroxyl-amin eller fortyndede syrer.
22. Anvendelse ifølge krav 18 eller 21, hvor kemikaliet er cynogenbromid.
23. Anvendelse ifølge krav 18 eller 20, hvor de enzymatiske spaltningssteder er de, der genkendes af enterokinase, trypsin, chymotrypsin, elastase, pepsin, papain, subtilisin, thermolysin, V8 protease, endoproteinase Arg C (submaxillaris protease), clostripain, thrombin, collagenase, lysobacter enzymogenes (Lys C), mysobacter AI-1 protease eller faktor Xa.
24. Anvendelse ifølge krav 23, hvor enzymet er enterokinase.
25. Anvendelse ifølge et hvilket som helst af de foregående krav, som endvidere omfatter trinene: a) at klone genet, der koder fusionsproduktkonstruktionen, i en egnet ekspressionsvektor; b) at omdanne egnede værtsceller med den ovennævnte ekspressionsvektor; c) at udtrykke det ønskede peptid som et fusionsprotein i E. coli-værtsceller; d) at bryde værtscellerne og indsamle fusionsproteinet som inklusionslegemer; e) at separere fusionsproteinet, der indeholder inklusionslegemer, fra andre værtskomponenter; f) at solubilisere fusionsproteinet med et egnet denatureringsmiddel; g) at spalte spaltningsstedet med et egnet enzym eller kemikalie til frigivelse af fusionspeptidet; og h) at oprense fusionspeptidet fra reaktionsblandingen.
26. Anvendelse ifølge krav 1, hvor GCSF-fusionspartneren kodes af nucleotid-sekvensen, der er angivet i sekvens ID No 1 og 2.
27. Fusionsprodukt, der udtrykkes ved processen ifølge et hvilket som helst af de foregående krav, hvor fusionsproduktet har formlen fusionspartner-CS-fusionspeptid hvor fusionspeptidet er et peptid af interesse, CS er et egnet spaltningssted, og fusionspartneren er GCSF, og hvor peptidet er valgt blandt parathyreoideahor-mon (PTH) (1-34), angiotensin og proinsulin.
28. Ekspressionsvektor, der er valgt blandt pET27B-GCSF-PTH, som er deponeret under MTCC tilgangsnummer 5425, pET27B-GCSF-proinsuin, som er deponeret under MTCC tilgangsnummer 5424, og pET27B-GCSF-angiotensin, der er en ekspressionsvektor pET27B, i hvilket der er indført en GCSF-angioten- sionfusionsproteinkodende sekvens, som er angivet i sekvens ID No 16 og 17.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IN1848MU2007 | 2007-09-21 | ||
| PCT/IN2008/000599 WO2009066320A2 (en) | 2007-09-21 | 2008-09-22 | Fusion protein systems suitable for high expression of peptides |
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| DK2201120T3 true DK2201120T3 (da) | 2014-03-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| DK08852235.4T DK2201120T3 (da) | 2007-09-21 | 2008-09-22 | GCSF-fusionsproteinsystemer, der er egnet til høj ekspression af peptider |
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| EP (2) | EP2201120B1 (da) |
| JP (1) | JP5368450B2 (da) |
| AU (1) | AU2008327513B2 (da) |
| DK (1) | DK2201120T3 (da) |
| PL (1) | PL2201120T3 (da) |
| WO (1) | WO2009066320A2 (da) |
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| EP2499255A4 (en) * | 2009-11-09 | 2013-06-19 | Univ Colorado Regents | EFFICIENT PRODUCTION OF PEPTIDES |
| WO2018152371A1 (en) * | 2017-02-15 | 2018-08-23 | Fred Hutchinson Cancer Research Center | In vivo gene therapy for immune deficiencies |
| CN106967663B (zh) * | 2017-05-06 | 2020-08-14 | 中国海洋大学 | 一种用于农作物病害防治的重组菌株 |
| EP3630807B1 (en) | 2017-05-25 | 2025-08-13 | Leidos, Inc. | Pd-1 and ctla-4 dual inhibitor peptides |
| WO2019130344A1 (en) * | 2017-12-27 | 2019-07-04 | Council Of Scientific And Industrial Research | A polypeptide exhibiting granulocyte-colony stimulating factor activity |
| WO2019143193A1 (ko) | 2018-01-19 | 2019-07-25 | 주식회사 펩진 | 재조합 폴리펩타이드 생산용 n-말단 융합 파트너 및 이를 이용하여 재조합 폴리 펩타이드를 생산하는방법 |
| JP7606471B2 (ja) | 2019-05-22 | 2024-12-25 | レイドス， インコーポレイテッド | Ｌａｇ３結合ペプチド |
| RU2728611C1 (ru) * | 2019-05-24 | 2020-07-30 | Закрытое акционерное общество "ФАРМ-ХОЛДИНГ" | РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК pF265, КОДИРУЮЩАЯ ГИБРИДНЫЙ ПОЛИПЕПТИД, СОДЕРЖАЩИЙ ПРОИНСУЛИН ЧЕЛОВЕКА, И ШТАММ БАКТЕРИЙ Escherichia coli - ПРОДУЦЕНТ ГИБРИДНОГО ПОЛИПЕПТИДА, СОДЕРЖАЩЕГО ПРОИНСУЛИН ЧЕЛОВЕКА |
| US11578102B2 (en) | 2020-07-31 | 2023-02-14 | Leidos, Inc. | LAG3 binding peptides |
| CN116731126B (zh) * | 2023-01-30 | 2024-02-23 | 态创生物科技(广州)有限公司 | 内含肽ChiATP、内含肽ChiATP-二肽-2融合蛋白及二肽-2的表达方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ199391A (en) | 1981-01-02 | 1985-12-13 | Genentech Inc | Chimeric polypeptides comprising a proinsulin sequence,and preparation by recombinant dna technique;production of human insulin |
| DE3526995A1 (de) | 1985-07-27 | 1987-02-05 | Hoechst Ag | Fusionsproteine, verfahren zu ihrer herstellung und ihre verwendung |
| US5223404A (en) | 1988-01-20 | 1993-06-29 | Sunstar Kabushiki Kaisha | Composition for testing periodontal diseases |
| JP3531947B2 (ja) | 1991-08-19 | 2004-05-31 | 第一サントリーファーマ株式会社 | ペプチドの製造方法 |
| US5738849A (en) * | 1992-11-24 | 1998-04-14 | G. D. Searle & Co. | Interleukin-3 (IL-3) variant fusion proteins, their recombinant production, and therapeutic compositions comprising them |
| KR100230578B1 (ko) | 1997-07-25 | 1999-12-01 | 허영섭 | 포스포리불로키나제를 융합파트너로 이용하는 재조합 인간 부갑상선호르몬의 발현벡터 |
| WO2007091250A2 (en) | 2006-02-06 | 2007-08-16 | The Medical Research And Infrastructure Fund Of The Tel-Aviv Sourasky Medical Center | Enzyme replacement therapy for treating lysosomal storage diseases |
| AU2007224331B2 (en) | 2006-03-06 | 2011-05-26 | Cadila Healthcare Limited | Process for preparing human G-CSF |
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2008
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- 2008-09-22 AU AU2008327513A patent/AU2008327513B2/en not_active Ceased
- 2008-09-22 EP EP08852235.4A patent/EP2201120B1/en active Active
- 2008-09-22 EP EP12179359.0A patent/EP2522728A1/en not_active Withdrawn
- 2008-09-22 US US12/679,346 patent/US8507221B2/en not_active Expired - Fee Related
- 2008-09-22 JP JP2010525497A patent/JP5368450B2/ja not_active Expired - Fee Related
- 2008-09-22 PL PL08852235T patent/PL2201120T3/pl unknown
- 2008-09-22 WO PCT/IN2008/000599 patent/WO2009066320A2/en not_active Ceased
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| JP2010539898A (ja) | 2010-12-24 |
| EP2201120A2 (en) | 2010-06-30 |
| WO2009066320A2 (en) | 2009-05-28 |
| US20120142891A1 (en) | 2012-06-07 |
| WO2009066320A3 (en) | 2009-09-24 |
| AU2008327513B2 (en) | 2012-05-03 |
| US8507221B2 (en) | 2013-08-13 |
| AU2008327513A2 (en) | 2010-06-17 |
| PL2201120T3 (pl) | 2014-05-30 |
| JP5368450B2 (ja) | 2013-12-18 |
| EP2201120B1 (en) | 2013-12-25 |
| EP2522728A1 (en) | 2012-11-14 |
| AU2008327513A1 (en) | 2009-05-28 |
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