DK2205662T3 - Partikler, som stammer fra en emulsion - Google Patents
Partikler, som stammer fra en emulsion Download PDFInfo
- Publication number
- DK2205662T3 DK2205662T3 DK08845633.0T DK08845633T DK2205662T3 DK 2205662 T3 DK2205662 T3 DK 2205662T3 DK 08845633 T DK08845633 T DK 08845633T DK 2205662 T3 DK2205662 T3 DK 2205662T3
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- DK
- Denmark
- Prior art keywords
- particles
- emulsion
- protein
- particle
- proteins
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- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 1
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 1
- FEMOMIGRRWSMCU-UHFFFAOYSA-N ninhydrin Chemical compound C1=CC=C2C(=O)C(O)(O)C(=O)C2=C1 FEMOMIGRRWSMCU-UHFFFAOYSA-N 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
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- 125000000636 p-nitrophenyl group Chemical group [H]C1=C([H])C(=C([H])C([H])=C1*)[N+]([O-])=O 0.000 description 1
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- 150000003141 primary amines Chemical class 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 230000006916 protein interaction Effects 0.000 description 1
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- 239000000741 silica gel Substances 0.000 description 1
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- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- HLBBKKJFGFRGMU-UHFFFAOYSA-M sodium formate Chemical compound [Na+].[O-]C=O HLBBKKJFGFRGMU-UHFFFAOYSA-M 0.000 description 1
- 235000019254 sodium formate Nutrition 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
- C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/12—Powdering or granulating
- C08J3/16—Powdering or granulating by coagulating dispersions
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K17/00—Carrier-bound or immobilised peptides; Preparation thereof
- C07K17/02—Peptides being immobilised on, or in, an organic carrier
- C07K17/08—Peptides being immobilised on, or in, an organic carrier the carrier being a synthetic polymer
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/005—Processes for mixing polymers
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/24—Crosslinking, e.g. vulcanising, of macromolecules
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/06—Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2379/00—Characterised by the use of macromolecular compounds obtained by reactions forming in the main chain of the macromolecule a linkage containing nitrogen with or without oxygen, or carbon only, not provided for in groups C08J2361/00 - C08J2377/00
- C08J2379/02—Polyamines
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Polymers & Plastics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Dispersion Chemistry (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicinal Preparation (AREA)
Claims (13)
1
1. Partikel, som stammer fra en emulsion og omfatter et gitter af polyethyle-neimin-strenge, som er tværbundet ved hjælp af et tværbindingsmiddel, in-terstitielle åbninger, som grænser op til strengene og befinder sig omkring dem, og funktionelle grupper på gitteret, og med hvilke proteiner og/eller modificerede proteiner kan reagere, således at de kan bindes til gitteret og derved immobiliseret.
2. Partikel ifølge krav 1, hvor de funktionelle grupper er til stede på polyethy-leneiminen, og er udvalgt, således at binding af proteinerne og/eller de modificerede proteiner til polyethyleneiminen kan udføres gennem en eller flere af kovalent binding, ionisk binding, hydrofobisk binding og affinitetsbinding, som kan udføres gennem modifikation af gitteret for at tilvejebringe denne funktio-nalitet.
3. Partikel ifølge krav 1 eller krav 2, som indeholder mindst ét protein og/eller modificeret protein, der er bundet til gitteret ved hjælp af de funktionelle grupper, hvorved de immobiliseres.
4. Partikel ifølge krav 3, hvor en flerhed af forskellige proteiner og/eller en flerhed af forskellige modificerede proteiner er immobiliseret deri.
5. Partikel ifølge et hvilket som helst af kravene 1 til 4 inklusive, som indeholder et tilsætningsmiddel, som er indfanget i gitteret.
6. Partikel ifølge krav 5, hvor tilsætningsmidlet er udvalgt blandt en co-faktor, en modificeret co-faktor, en kemisk mediator, magnetit og en magnetisk substans.
7. Fremgangsmåde til fremstilling af partikler, som omfatter at tilvejebringe en emulsion af små dråber af en første flydende fase, som dispergeres i en anden flydende fase, hvor den ene flydende fase er en vandig fase, og den anden er en oliefase, og hvor den vandige fase indeholder polyethyleneimin, der er opløst deri, samt et tværbindingsmiddel, som er opløst deri; 2 at gøre det muligt for tværbindingsmidlet at tværbinde strenge af polyethyle-neimin, for derved at danne partikler, som hver især omfatter et gitter af strenge af polyethyleneimin, som er tværbundet ved hjælp af tværbindingsmidlet, interstitielle åbninger, som grænser op til strengene og befinder sig omkring dem, og funktionelle grupper på gitteret, og med hvilke proteiner og/eller modificerede proteiner kan reagere, således at de kan bindes til gitteret og derved immobiliseret.
8. Fremgangsmåde ifølge krav 7, hvor emulsionen dannes ved at blande en første emulsion, som omfatter vandige små dråber, der indeholder deri opløst polyethyleneimin, og som dispergeres i en oliefase, med en anden emulsion, som omfatter vandige små dråber, der indeholder tværbindingsmidlet, der er opløst deri, og som er dispergeret i en oliefase.
9. Fremgangsmåde ifølge krav 7 eller krav 8, som omfatter at tilsætte et tilsætningsmiddel til mindst én af faserne, således at tilsætningsmidlet indfanges i partiklernes gitre.
10. Fremgangsmåde ifølge krav 9, hvor tilsætningsmidlet er udvalgt blandt en co-faktor, en modificeret co-faktor, en kemisk mediator, magnetit og en magnetisk substans.
11. Fremgangsmåde ifølge et hvilket som helst af kravene 7 til 10 inklusive, som omfatter genvinding af partiklerne og tørring af de genvundne partikler.
12. Fremgangsmåde ifølge krav 11, som omfatter at tilsætte et tilsætningsmiddel til de genvundne partikler inden tørringen af partiklerne, således at tilsætningsmidlet indfanges i partiklernes gitre.
13. Fremgangsmåde ifølge krav 12, hvor tilsætningsmidlet er udvalgt blandt en co-faktor, en modificeret co-faktor, en kemisk mediator, magnetit og en magnetisk substans.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ZA200709300 | 2007-10-29 | ||
| PCT/IB2008/054458 WO2009057049A2 (en) | 2007-10-29 | 2008-10-29 | Emulsion-derived particles |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2205662T3 true DK2205662T3 (da) | 2016-10-10 |
Family
ID=40591572
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK08845633.0T DK2205662T3 (da) | 2007-10-29 | 2008-10-29 | Partikler, som stammer fra en emulsion |
Country Status (9)
| Country | Link |
|---|---|
| US (3) | US20100267108A1 (da) |
| EP (1) | EP2205662B1 (da) |
| JP (1) | JP5442624B2 (da) |
| CN (1) | CN101874061B (da) |
| BR (1) | BRPI0819130B1 (da) |
| CA (1) | CA2703478C (da) |
| DK (1) | DK2205662T3 (da) |
| ES (1) | ES2595104T3 (da) |
| WO (1) | WO2009057049A2 (da) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9567426B2 (en) * | 2009-05-29 | 2017-02-14 | Cytec Technology Corp. | Engineered crosslinked thermoplastic particles for interlaminar toughening |
| CN103111247A (zh) * | 2013-02-01 | 2013-05-22 | 江南大学 | 一种聚胺基微球的制备方法 |
| AU2016252393A1 (en) * | 2015-04-24 | 2017-11-09 | Colgate-Palmolive Company | Porous protein particles as carriers for actives |
| EP3323166B1 (en) * | 2015-07-15 | 2024-03-13 | ZYMtronix, Inc. | Automated bionanocatalyst production |
| KR101863289B1 (ko) * | 2016-06-01 | 2018-06-01 | 한국생산기술연구원 | 이산화탄소 흡착제용 글루타르알데히드 가교된 pei 입자의 제조방법 및 이에 따라 제조한 글루타르알데히드 가교된 pei 입자 |
| US20210072255A1 (en) | 2016-12-16 | 2021-03-11 | The Brigham And Women's Hospital, Inc. | System and method for protein corona sensor array for early detection of diseases |
| US20180346648A1 (en) * | 2017-05-30 | 2018-12-06 | International Flavors & Fragrances Inc. | Branched polyethyleneimine microcapsules |
| CN109320972A (zh) * | 2018-09-27 | 2019-02-12 | 江南大学 | 一种多孔聚乙烯亚胺微球的制备方法 |
| KR20230155589A (ko) | 2018-11-07 | 2023-11-10 | 시어 인코퍼레이티드 | 단백질 코로나 분석을 위한 조성물, 방법 및 시스템 및 그것들의 용도 |
| CN109355277A (zh) * | 2018-11-12 | 2019-02-19 | 南京启佑生物科技有限公司 | 一种交联固定化脂肪酶-高分子材料复合物的制备方法及其应用 |
| JP7518852B2 (ja) | 2019-03-26 | 2024-07-18 | シアー， インコーポレイテッド | 生物流体からのタンパク質コロナ分析のための組成物、方法およびシステム、ならびにそれらの使用 |
| CN117169534A (zh) | 2019-08-05 | 2023-12-05 | 禧尔公司 | 用于样品制备、数据生成和蛋白质冠分析的系统和方法 |
| JP2023540904A (ja) | 2020-08-25 | 2023-09-27 | シアー， インコーポレイテッド | タンパク質および核酸をアッセイするための組成物および方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4141857A (en) * | 1976-04-30 | 1979-02-27 | Uop Inc. | Support matrices for immobilized enzymes |
| US4415663A (en) * | 1982-08-25 | 1983-11-15 | Uop Inc. | Support matrix for immobilized enzymes |
| US4713333A (en) * | 1985-09-23 | 1987-12-15 | Miles Laboratories, Inc. | Immobilization of biocatalysts on granular diatomaceous earth |
| DE3728772A1 (de) * | 1987-08-28 | 1989-03-09 | Hoechst Ag | Enzymreaktor mit polymerfixiertem cofaktor |
| US5216096A (en) * | 1991-09-24 | 1993-06-01 | Japan Synthetic Rubber Co., Ltd. | Process for the preparation of cross-linked polymer particles |
| US6689615B1 (en) * | 2000-10-04 | 2004-02-10 | James Murto | Methods and devices for processing blood samples |
| EP1647559B1 (en) * | 2004-10-14 | 2012-04-04 | Tosoh Corporation | Crosslinked (meth) acrylamide particles, process for their production and their use |
-
2008
- 2008-10-29 US US12/740,123 patent/US20100267108A1/en not_active Abandoned
- 2008-10-29 WO PCT/IB2008/054458 patent/WO2009057049A2/en not_active Ceased
- 2008-10-29 ES ES08845633.0T patent/ES2595104T3/es active Active
- 2008-10-29 BR BRPI0819130-1A patent/BRPI0819130B1/pt active IP Right Grant
- 2008-10-29 DK DK08845633.0T patent/DK2205662T3/da active
- 2008-10-29 CA CA2703478A patent/CA2703478C/en active Active
- 2008-10-29 JP JP2010531623A patent/JP5442624B2/ja active Active
- 2008-10-29 CN CN200880114052.4A patent/CN101874061B/zh active Active
- 2008-10-29 EP EP08845633.0A patent/EP2205662B1/en active Active
-
2013
- 2013-02-11 US US13/763,919 patent/US20130149730A1/en not_active Abandoned
-
2015
- 2015-11-18 US US14/945,002 patent/US9574054B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN101874061B (zh) | 2014-09-17 |
| US20130149730A1 (en) | 2013-06-13 |
| EP2205662A2 (en) | 2010-07-14 |
| US20160068641A1 (en) | 2016-03-10 |
| JP5442624B2 (ja) | 2014-03-12 |
| WO2009057049A2 (en) | 2009-05-07 |
| CA2703478C (en) | 2015-07-07 |
| BRPI0819130B1 (pt) | 2019-09-17 |
| CA2703478A1 (en) | 2009-05-07 |
| US9574054B2 (en) | 2017-02-21 |
| JP2011500099A (ja) | 2011-01-06 |
| CN101874061A (zh) | 2010-10-27 |
| ES2595104T3 (es) | 2016-12-27 |
| US20100267108A1 (en) | 2010-10-21 |
| WO2009057049A3 (en) | 2009-11-05 |
| EP2205662B1 (en) | 2016-07-13 |
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