DK2210085T3 - Apparat og fremgangsmåde til elektrokemisk detektering - Google Patents
Apparat og fremgangsmåde til elektrokemisk detektering Download PDFInfo
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- DK2210085T3 DK2210085T3 DK08842920.4T DK08842920T DK2210085T3 DK 2210085 T3 DK2210085 T3 DK 2210085T3 DK 08842920 T DK08842920 T DK 08842920T DK 2210085 T3 DK2210085 T3 DK 2210085T3
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- Prior art keywords
- electrode
- sensor
- electrochemical
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- electrodes
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- 238000000835 electrochemical detection Methods 0.000 title description 2
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3273—Devices therefor, e.g. test element readers, circuitry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
- G01N27/021—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance before and after chemical transformation of the material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
- G01N33/4905—Determining clotting time of blood
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/4738—Diffuse reflection, e.g. also for testing fluids, fibrous materials
- G01N2021/4776—Miscellaneous in diffuse reflection devices
- G01N2021/478—Application in testing analytical test strips
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/974—Thrombin
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- Chemical & Material Sciences (AREA)
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- Immunology (AREA)
- Molecular Biology (AREA)
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- Biomedical Technology (AREA)
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- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
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- Food Science & Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
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- Wood Science & Technology (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
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- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Claims (14)
1. Sensor med modstående elektroder, hvilken sensor omfatter en arbejdselektrode, en modelektrode, thromboplastin og et elektrokemisk thrombinsubstrat, hvor thromboplastinet er overtrukket på den ene af elektroderne, og det elektrokemiske thrombinsubstrat er overtrukket på den anden elektrode, hvor sensoren detekterer en spaltning af det elektrokemiske thrombinsubstrat.
2. Sensor ifølge krav 1, hvor det elektrokemiske thrombinsubstrat ikke er immobiliseret på arbejdselektroden.
3. Sensor ifølge krav 1 eller krav 2, hvilken sensor er kendetegnet ved, at det elektrokemiske thrombinsubstrat omfatter et peptid, der er bundet til en fraspaltelig enhed, hvor den fraspaltelige enhed bliver elektroaktiv efter spaltningen med thrombin.
4. Sensor ifølge et hvilket som helst af kravene 1-3, hvilken sensor yderligere omfatter en måler, der er forbundet med sensoren, hvor måleren bestemmer en koaguleringstid for en prøve.
5. Sensor ifølge krav 4, hvor måleren viser eller udlæser et resultat, der omfatter koaguleringstiden, eller et resultat eller en værdi, som er afhængig af koaguleringstiden.
6. Sensor ifølge et hvilket som helst af de foregående krav, hvilken sensor yderligere omfatter en måler, der er forbundet med sensoren, hvor måleren viser eller udlæser et resultat, som omfatter en parameter for thrombindannelse i prøven.
7. Teststrimmel til måling af blod- eller plasmakoaguleringstiden i en prøve, hvilken teststrimmel omfatter en sensor ifølge et hvilket som helst af kravene 1-6.
8. Teststrimmel ifølge krav 7, hvilken teststrimmel yderligere omfatter et middel til bekræftelse af strimmelintegriteten.
9. Fremgangsmåde til måling af blod- eller plasmakoagulering, hvilken fremgangsmåde omfatter: tilføring af en blodprøve fra en patient til en sensor med modstående elektroder, hvilken sensor omfatter en arbejdselektrode, en modelektrode, thromboplastin og et elektrokemisk thrombinsubstrat, hvilken fremgangsmåde er kendetegnet ved, at thromboplastinet er overtrukket på den ene af elektroderne, og det elektrokemiske thrombinsubstrat er overtrukket på den anden elektrode, og at sensoren detekterer en spaltning af det elektrokemiske thrombinsubstrat; og analyse af prøven ved hjælp af sensoren til bestemmelse af niveauet af spaltning for mindst at bestemme én blod- eller plasmakoaguleringsegenskab i prøven.
10. Fremgangsmåde ifølge krav 9, hvor fremgangsmåden gennemføres på behandlingsstedet.
11. Fremgangsmåde ifølge krav 9 eller krav 10, hvor fremgangsmåden yderligere omfatter en måler, der er forbundet med sensoren, hvor måleren viser eller udlæser et resultat, der omfatter en parameter for thrombindannelse i prøven.
12. Fremgangsmåde ifølge et hvilket som helst af kravene 9-11, hvor patienten tager et antikoaguleringsmedikament.
13. Fremgangsmåde ifølge krav 12, hvor antikoaguleringsmedikamentet er en vitamin K-antagonist.
14. Fremgangsmåde ifølge et hvilket som helst af kravene 9-13, hvor prøven fra patienten tages ved hjælp af et prik i patientens finger.
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| CA2704126C (en) | 2017-07-25 |
| IL205315A0 (en) | 2010-12-30 |
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| AU2008315677B2 (en) | 2014-04-17 |
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| SG171582A1 (en) | 2011-06-29 |
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| CN102232185A (zh) | 2011-11-02 |
| WO2009053834A1 (en) | 2009-04-30 |
| TW200942619A (en) | 2009-10-16 |
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| PL2210085T3 (pl) | 2016-05-31 |
| ES2557933T3 (es) | 2016-01-29 |
| JP6147463B2 (ja) | 2017-06-14 |
| MX359616B (es) | 2018-10-03 |
| JP2016048248A (ja) | 2016-04-07 |
| TWI516601B (zh) | 2016-01-11 |
| CN102232185B (zh) | 2016-08-03 |
| NZ585031A (en) | 2012-12-21 |
| PT2210085E (pt) | 2016-01-29 |
| MX2010004465A (es) | 2010-10-25 |
| KR101854883B1 (ko) | 2018-05-04 |
| HUE026083T2 (en) | 2016-05-30 |
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| JP2011501181A (ja) | 2011-01-06 |
| ZA201002856B (en) | 2011-03-30 |
| KR20100087181A (ko) | 2010-08-03 |
| US20100252452A1 (en) | 2010-10-07 |
| EP2210085A1 (en) | 2010-07-28 |
| SI2210085T1 (sl) | 2016-04-29 |
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