DK2257155T3 - Kryokonservering af biologiske celler og væv - Google Patents

Kryokonservering af biologiske celler og væv Download PDF

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Publication number
DK2257155T3
DK2257155T3 DK09714696.3T DK09714696T DK2257155T3 DK 2257155 T3 DK2257155 T3 DK 2257155T3 DK 09714696 T DK09714696 T DK 09714696T DK 2257155 T3 DK2257155 T3 DK 2257155T3
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DK
Denmark
Prior art keywords
cell
holder
cell holder
station
tray
Prior art date
Application number
DK09714696.3T
Other languages
English (en)
Inventor
Tomas Stojanov
Original Assignee
Genea Ip Holdings Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2008900890A external-priority patent/AU2008900890A0/en
Application filed by Genea Ip Holdings Pty Ltd filed Critical Genea Ip Holdings Pty Ltd
Application granted granted Critical
Publication of DK2257155T3 publication Critical patent/DK2257155T3/da

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/14—Mechanical aspects of preservation; Apparatus or containers therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/14—Mechanical aspects of preservation; Apparatus or containers therefor
    • A01N1/146—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving
    • A01N1/147—Carriers for immersion in cryogenic fluid for slow freezing or vitrification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Rigid containers without fluid transport within
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/50—Cryostats
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1838—Means for temperature control using fluid heat transfer medium
    • B01L2300/1844—Means for temperature control using fluid heat transfer medium using fans
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/42—Low-temperature sample treatment, e.g. cryofixation

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Claims (14)

1. Fremgangsmåde til kryokonservering afen organisk celle (7) som anvendt i forbindelse med in-vitro fertiliseringsprocedurer, hvilken fremgangsmåde indbefatter følgende trin: tilvejebringelse af en organisk celle til kryokonservering; opbevaring af cellen i en celleholder (1); fastgørelse af celleholderen (1), der indeholder cellen, ved en behandlingsstation (30, 40), hvor celleholderen (1) er fastgjort i en celleholderramme (20) og rammen (20) er fastgjort til behandlingsstationen (30, 40), hvilken ramme (20) indbefatter en indgrebsformation (21) til at bringe celleholderen (1) i indgreb, hvorved celleholderen (1) fastgøres til rammen (20), hvilken behandlingsstation (30, 40) indbefatter koblingsformationer udformet med tilsvarende koblingsformationer på rammen (20) til at fastgøre rammen (20) til behandlingsstationen (30, 40) i en forudbestemt orientering; påføring afen første behandlingsopløsning til cellen; afkøling af celleholderen (1) og cellen til en forudbestemt kryokonserveringstemperatur; og opbevaring af cellen ved eller under en opbevaringstemperatur, hvor cellen afkøles ved en afkølingshastighed, der er tilstrækkelig til at fremme vitrificering af cellen og den omgivende behandlingsopløsning, og hvor trinnet med påføring afen behandlingsopløsning indbefatter tilbageholdelse af cellen i celleholderen (1) og at tillade, at opløsningen strømmer hen over cellen.
2. Fremgangsmåde ifølge krav 1, hvor celleholderen (1) indbefatter et filtreringsarrangement (2), der gør det muligt for fluid at passere gennem filtreringsarrangementet, mens cellen tilbageholdes
3. Fremgangsmåde ifølge krav 2, hvor celleholderen (1) indbefatter mindst én sidevæg (3), hvorved der dannes en kopformet opbevaringsbeholder (4) og defineres en åben indgang (5).
4. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor trinnet med påføring afen behandlingsopløsning indbefatter sekventiel påføring af første og anden behandlingsopløsning til cellen.
5. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 3, hvor trinnet med påføring afen behandlingsopløsning indbefatter sekventiel påføring afen flerhed af behandlingsopløsninger til cellen.
6. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor behandlingsopløsningen er en vitrificeringsopløsning.
7. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor en flerhed af celleholderne (1) fastgøres samtidigt i et system ved behandlingsstationen (30, 40).
8. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor den organiske celle indbefatter én af en oocyt, en stemcelle og et embryo.
9. Apparat til kryokonservering af organisk celle til anvendelse i vitrificeringen afen organisk celle (7), hvilket apparat omfatter: en celleholder (1) med et filtreringsarrangement (2) til at tilbageholde den organiske celle, mens det tillades, at fluid passerer fra celleholderen (1); en celleholderramme (20), der omfatter en indgrebsformation til at bringe celleholderen (1) i indgreb og fastholde den i en forudbestemt orientering i forhold til celleholderrammen (20); en behandlingsstation (30) til vask af den organiske celle, hvilken behandlingsstation indbefatter: et temperaturstyringsarrangement (32); og et koblingsarrangement til at bringe et tilsvarende koblingsarrangement i indgreb på celleholderrammen (20), hvorved celleholderrammen (20) flugter i en forudbestemt orientering med stationen (30), hvor apparatet endvidere indbefatter en kølestation (40) til afkøling af cellerne til en vitrificeringstemperatur; og hvor kølestationen indbefatter en flydende nitrogenafkølet blok.
10. Apparat ifølge krav 9, hvor celleholderen (1) indbefatter et filtreringsarrangement (2) til at tilbageholde cellen, mens det tillades, at fluid passerer gennem filtreringsarrangementet (2) og fra holderen.
11. Apparat ifølge krav 9 eller 10, hvor celleholderen (1) indbefatter mindst én sidevæg (3), hvorved der dannes en kopformet opbevaringsbeholder (4) og defineres en åben indgang (5).
12. Apparat ifølge krav 11, hvor den mindst ene sidevæg (3) indbefatter filtreringsarrangementet (2).
13. Apparat ifølge et hvilket som helst af kravene 10 til 12, hvor filtreringsarrangementet (2) indbefatter mindst én af et net; en membran; et fibermateriale og en perforeret sektion.
14. Apparat ifølge et hvilket som helst af kravene 9 til 13, der endvidere indbefatter et aflangt greb (8) til håndtering af celleholderen (1).
DK09714696.3T 2008-02-25 2009-02-25 Kryokonservering af biologiske celler og væv DK2257155T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
AU2008900890A AU2008900890A0 (en) 2008-02-25 Cryopreservation of biological cells and tissues
PCT/AU2009/000212 WO2009105813A1 (en) 2008-02-25 2009-02-25 Cryopreservation of biological cells and tissues

Publications (1)

Publication Number Publication Date
DK2257155T3 true DK2257155T3 (da) 2019-01-21

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
DK09714696.3T DK2257155T3 (da) 2008-02-25 2009-02-25 Kryokonservering af biologiske celler og væv

Country Status (10)

Country Link
US (1) US8859283B2 (da)
EP (2) EP2257155B1 (da)
AU (1) AU2009219096A1 (da)
DK (1) DK2257155T3 (da)
ES (1) ES2706623T3 (da)
HU (1) HUE041510T2 (da)
PL (1) PL2257155T3 (da)
PT (1) PT2257155T (da)
TR (1) TR201820845T4 (da)
WO (1) WO2009105813A1 (da)

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US9228925B2 (en) 2011-08-04 2016-01-05 University Of Kansas Automated vitrification device
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JP5798633B2 (ja) 2011-10-04 2015-10-21 株式会社北里バイオファルマ 細胞凍結保存用具
JP5798634B2 (ja) 2011-10-05 2015-10-21 株式会社北里バイオファルマ 生体細胞凍結保存用具
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Also Published As

Publication number Publication date
US8859283B2 (en) 2014-10-14
HUE041510T2 (hu) 2019-05-28
PT2257155T (pt) 2019-02-08
TR201820845T4 (tr) 2019-01-21
EP3510865A2 (en) 2019-07-17
PL2257155T3 (pl) 2019-05-31
WO2009105813A1 (en) 2009-09-03
EP2257155A4 (en) 2015-11-11
ES2706623T3 (es) 2019-03-29
EP3510865A3 (en) 2020-01-15
EP2257155A1 (en) 2010-12-08
AU2009219096A1 (en) 2009-09-03
US20100317108A1 (en) 2010-12-16
EP2257155B1 (en) 2018-11-21

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